Traditional Chinese medicine composition and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine composition preparation, and particularly discloses a traditional Chinese medicine composition and a preparation method thereof.The traditional Chinese medicine composition is prepared from 25-35 parts of radix astragali, 25-35 parts of dried dendrobe, 25-35 parts of radix puerariae, 1-5 parts of pseudo-ginseng powder, 25-35 parts of radix salviae miltiorrhizae, 4-8 parts of rhizoma coptidis tablets and 10-20 parts of lophatherum gracile. The astragalus membranaceus in the traditional Chinese medicine composition can tonify qi and raise yang, consolidate superficies and stop sweating, and is mainly used for tonifying qi and strengthening the body resistance; the dried dendrobium nobile has the effects of nourishing yin, clearing heat, tonifying stomach and promoting fluid, and is matched with the astragalus membranaceus to tonify both qi and yin and harmonize yin and yang; the radix puerariae is cooperated with the radix astragali and the herba dendrobii, so that the effects of tonifying qi and nourishing yin are enhanced; pseudo-ginseng powder has the characteristic of activating blood without hurting healthy qi, and helps astragalus membranaceus and dendrobe to dredge collaterals and remove blood stasis and improve qi and blood circulation. The radix salviae miltiorrhizae and the radix notoginseng powder are matched to enhance the effects of promoting blood circulation to remove meridian obstruction and clear away stagnant heat; due to the bitter and cold nature, the rhizoma coptidis tablets can clear internal heat and prevent heat generation due to excessive nourishing; the lophatherum gracile is used, so that the traditional Chinese medicine can clear heat without hurting yin and tonify without evil stagnation, and all the components are mutually cooperated and are scientifically proportioned.

Description

Technical Field

[0001] The present invention relates to the technical field of preparation of traditional Chinese medicine compositions, and in particular to a traditional Chinese medicine composition and a preparation method thereof. Background Art

[0002] Type 2 diabetes mellitus (T2DM) is a highly prevalent metabolic disease worldwide, with its core pathological characteristics being insulin resistance and decreased pancreatic β-cell function. Coronary heart disease (CHD) is the most common macrovascular complication of T2DM. When the two coexist, a vicious cycle of "metabolic abnormalities-vascular damage" is formed. Hyperglycemia accelerates coronary atherosclerosis through mechanisms such as the deposition of advanced glycation end products and enhanced oxidative stress, while insufficient coronary blood supply further aggravates pancreatic cell ischemia and hypoxia, leading to a deterioration in blood sugar regulation.

[0003] Existing traditional Chinese medicine combinations for T2DM or CHD do not fully consider the complex syndromes of the two diseases, resulting in problems of "single efficacy and unbalanced compatibility", which are specifically manifested as: 1. Incomplete coverage of syndrome types and limited efficacy: Traditional Chinese medicines that focus on lowering blood sugar (such as combinations containing astragalus and kudzu root) primarily aim to "replenish qi and promote fluid production." While these can improve dry mouth and fatigue, they lack blood-activating and blood-stasis-removing ingredients (such as salvia miltiorrhiza and Panax notoginseng) and are unable to inhibit coronary thrombosis, resulting in a low rate of relief for CHD symptoms (such as angina pectoris). Traditional Chinese medicine that focuses on protecting the heart (such as a combination containing Salvia miltiorrhiza and Chuanxiong) mostly focuses on "promoting blood circulation and removing blood stasis". Although it can improve myocardial blood supply, the dosage of bitter and cold heat-clearing drugs (such as Coptis chinensis) is too high, and it is not combined with mild medicinal ingredients such as Aralia dasyphylla, which can easily damage the already weak spleen and lung qi of T2DM patients, leading to abdominal distension and diarrhea. 2. Low dissolution rate of key active ingredients: Existing Chinese medicine decoction methods mostly use the process of "simple soaking for 30 minutes and single-fire decoction for 40-50 minutes", which does not optimize the solubility characteristics of core ingredients (such as astragaloside IV, tanshinone IIA, and dendrobium polysaccharides) required for patients with T2DM and CHD: Astragaloside IV is a water-soluble component and requires sufficient soaking time (≥45 minutes) to be fully dissolved; tanshinone IIA is a fat-soluble component and is easily degraded by long-term high-temperature decoction, and its retention rate is too low under the existing process; Dendrobium polysaccharide requires gradient temperature decoction (first boiling over high heat, then simmering over low heat) to destroy the cell wall. The existing single-temperature process results in its dissolution rate below 50%, which directly affects its protective effect on pancreatic beta cells.

[0004] Therefore, it is necessary to design a Chinese medicine composition to solve the problems of incomplete syndrome type coverage, limited efficacy, and low dissolution rate of key active ingredients of traditional Chinese medicine compositions. Summary of the Invention

[0005] In view of this, the present invention proposes a Chinese medicine composition to solve the problems of incomplete syndrome type coverage, limited efficacy, and low dissolution rate of key active ingredients in traditional Chinese medicine compositions.

[0006] In one aspect, the present invention provides a traditional Chinese medicine composition comprising the following components in parts by weight: 25-35 parts of Astragalus, 25-35 parts of dried Dendrobium, 25-35 parts of Pueraria, 1-5 parts of Panax notoginseng powder, 25-35 parts of Salvia miltiorrhiza, 4-8 parts of Coptis chinensis slices and 10-20 parts of Bambusoideae.

[0007] On the other hand, the present invention also provides a method for preparing a Chinese medicine composition, comprising the following steps: The astragalus root, dried dendrobium, kudzu root, notoginseng powder, salvia miltiorrhiza, coptis root slices and bamboo leaves are pre-treated and set aside; adding the pretreated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves into water for soaking, then directly boiling the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein for a first time, and filtering to obtain a first decoction and medicinal residues; Adding water to the medicinal residue for a second decoction, adding Panax notoginseng powder during the decoction, and filtering to obtain a second decoction; The first decoction and the second decoction are mixed and concentrated to obtain the traditional Chinese medicine composition.

[0008] Furthermore, the pretreatment is as follows: rinsing the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis chinensis slices and bamboo leaves with water twice, each time for 10 seconds, then cutting the salvia miltiorrhiza into 2-3 mm thick slices, and cutting the bamboo leaves into 5-8 cm long segments; The Panax notoginseng powder was passed through an 80-mesh sieve.

[0009] Furthermore, the soaking is specifically as follows: adding 10 times the weight of water to the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices and bamboo leaves, and soaking them at room temperature of 20-25°C for 45 minutes.

[0010] Furthermore, the first decoction is specifically as follows: heating the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein until boiling, then turning to low heat, maintaining a slight boil, and decocting for 60 minutes.

[0011] Furthermore, the second decoction is specifically as follows: adding 8 times the weight of water to the medicinal residue, heating over high heat until boiling, then turning to low heat, and maintaining a slight boil and decocting for 40 minutes.

[0012] Furthermore, the Panax notoginseng powder is added 10 minutes before the end of the second decoction and stirred for 5 minutes.

[0013] Furthermore, the concentration is specifically carried out by heating the mixed decoction over low heat to concentrate it.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The Chinese medicine composition of the present invention is sweet and slightly warm in nature, can replenish qi and raise yang, consolidate the exterior and stop sweating, and is mainly used to replenish qi and strengthen the body, which is the core of the composition; dried dendrobium nourishes yin and clears heat, benefits the stomach and produces fluid, and is matched with astragalus to replenish qi and yin and harmonize yin and yang; kudzu root relieves muscles and reduces fever, produces fluid and quenches thirst, and promotes menstruation and activates collaterals, and cooperates with astragalus and dendrobium to enhance the function of replenishing qi and nourishing yin; Panax notoginseng powder can dissipate blood stasis and stop bleeding, promote blood circulation and relieve pain, and with its characteristics of promoting blood circulation without damaging the body, it helps astragalus and dendrobium to dredge the collaterals and remove blood stasis, and improve the circulation of qi and blood; Ginseng promotes blood circulation and removes blood stasis, clears the heart and relieves restlessness, and is combined with Panax notoginseng powder to enhance the effect of promoting blood circulation and unblocking meridians, and also clears blood stasis and heat; Coptis chinensis tablets are bitter and cold in nature, clear away heat and dampness, purge fire and detoxify, and with their bitter and cold nature, they can clear away internal heat and prevent excessive nourishment from generating heat; Aralia dasyphylla leaves clear heat and relieve restlessness, promote fluid production and promote urination, and lead heat downward, so that the whole formula clears heat without damaging yin, and nourishes without stagnating pathogens. The various components work together, so that the Chinese medicine composition of the present invention has the effects of both tonifying and purging, and together play the effects of invigorating qi and nourishing yin, promoting blood circulation and unblocking meridians, and clearing heat and promoting fluid production.

[0015] 2. The present invention significantly improves the dissolution rate of water-soluble core components such as astragaloside IV and dendrobium polysaccharide through the soaking process, and avoids the continuous high-temperature degradation of fat-soluble components such as tanshinone IIA through the gradient decoction process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. The same reference symbols are used throughout the drawings to represent the same components. In the drawings: Figure 1 A flow chart of a method for preparing a Chinese medicine composition provided in an embodiment of the present invention; Figure 2 The weight trend graph of each group of rats provided in the embodiment of the present invention; Figure 3 A trend chart of food intake and water intake of rats in each group provided in the embodiment of the present invention; Figure 4 The glucose metabolism and pancreatic islet function diagrams of each group of rats provided in the embodiments of the present invention; Figure 5 A graph showing blood lipid levels in rats in each group provided in the embodiments of the present invention; Figure 6 Cardiac function diagrams of rats in each group provided in the embodiments of the present invention; Figure 7 This is the HE staining result of the hearts of the rats in each group provided in the embodiment of the present invention; Figure 8 This is a diagram showing the pancreas HE staining results of each group of rats provided in the embodiments of the present invention; Figure 9 This is a graph showing the levels of ATP, NO, and ET-1 in the serum of rats in each group provided in the embodiments of the present invention; Figure 10 This is a diagram showing the expression of AMPK / SIRT1 / PGC-1α and p-AKT / GLUT4 signaling pathway proteins in the myocardial tissue of each group of rats provided in the examples of the present invention. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0018] In the description of this application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0019] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout this application, unless otherwise specified, "plurality" means two or more.

[0020] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed, detachable, or integral connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0021] Existing traditional Chinese medicine compositions for T2DM or CHD do not fully consider the complex syndromes of the two diseases, resulting in problems of "single efficacy and unbalanced compatibility", which are specifically manifested as: 1. Incomplete coverage of syndrome types and limited efficacy: Traditional Chinese medicines that focus on lowering blood sugar (such as combinations containing astragalus and kudzu root) primarily aim to "replenish qi and promote fluid production." While these can improve dry mouth and fatigue, they lack blood-activating and blood-stasis-removing ingredients (such as salvia miltiorrhiza and Panax notoginseng) and are unable to inhibit coronary thrombosis, resulting in a low rate of relief for CHD symptoms (such as angina pectoris). Traditional Chinese medicine that focuses on protecting the heart (such as a combination containing Salvia miltiorrhiza and Chuanxiong) mostly focuses on "promoting blood circulation and removing blood stasis". Although it can improve myocardial blood supply, the dosage of bitter and cold heat-clearing drugs (such as Coptis chinensis) is too high, and it is not combined with mild medicinal ingredients such as Aralia dasyphylla, which can easily damage the already weak spleen and lung qi of T2DM patients, leading to abdominal distension and diarrhea. 2. Low dissolution rate of key active ingredients: Existing Chinese medicine decoction methods mostly use the process of "simple soaking for 30 minutes and single-fire decoction for 40-50 minutes", which does not optimize the solubility characteristics of core ingredients (such as astragaloside IV, tanshinone IIA, and dendrobium polysaccharides) required for patients with T2DM and CHD: Astragaloside IV is a water-soluble component and requires sufficient soaking time (≥45 minutes) to be fully dissolved; tanshinone IIA is a fat-soluble component and is easily degraded by long-term high-temperature decoction, and its retention rate is too low under the existing process; Dendrobium polysaccharide requires gradient temperature decoction (first boiling over high heat, then simmering over low heat) to destroy the cell wall. The existing single-temperature process results in its dissolution rate below 50%, which directly affects its protective effect on pancreatic beta cells.

[0022] Therefore, it is necessary to design a Chinese medicine composition to solve the problems of incomplete syndrome type coverage, limited efficacy, and low dissolution rate of key active ingredients of traditional Chinese medicine compositions.

[0023] On the one hand, in some embodiments of the present application, a traditional Chinese medicine composition comprises the following components in parts by weight: 25-35 parts of Astragalus, 25-35 parts of dried Dendrobium, 25-35 parts of Pueraria, 1-5 parts of Panax notoginseng powder, 25-35 parts of Salvia miltiorrhiza, 4-8 parts of Coptis chinensis slices and 10-20 parts of Bambusoideae.

[0024] Preferably, 30 parts of Astragalus, 30 parts of dried Dendrobium, 30 parts of Pueraria, 3 parts of Panax notoginseng powder, 30 parts of Salvia miltiorrhiza, 6 parts of Coptis chinensis slices and 15 parts of Bambusoideae.

[0025] Specifically, the Coptis chinensis slices should exclude those that are moldy or worm-eaten.

[0026] It can be understood that astragalus is sweet and slightly warm in nature, replenishes qi and raises yang, consolidates the exterior and stops sweating, and is used to replenish qi and strengthen the body, which is the core of the composition; dried dendrobium nourishes yin and clears heat, benefits the stomach and produces fluid, and is matched with astragalus to replenish qi and yin and harmonize yin and yang; kudzu root relieves muscles and reduces fever, produces fluid and quenches thirst, and activates meridians, and works together with astragalus and dendrobium to enhance the effect of replenishing qi and nourishing yin; Panax notoginseng powder removes blood stasis and stops bleeding, activates blood circulation and relieves pain, and with its characteristics of activating blood circulation without damaging the body, it helps astragalus and dendrobium to dredge meridians and remove blood stasis, and improve the circulation of qi and blood; salvia miltiorrhiza activates blood circulation It can dispel blood stasis, clear the heart and relieve restlessness, and is matched with Panax notoginseng powder to enhance the effect of promoting blood circulation and unblocking meridians, and also clear away blood stasis and heat; Coptis chinensis tablets are bitter and cold in nature, clear away heat and dampness, purge fire and detoxify, and with their bitter and cold nature, they can clear away internal heat and prevent excessive nourishment from generating heat; Light bamboo leaves clear heat and relieve restlessness, promote fluid production and promote urination, and lead heat downward, so that the whole formula clears heat without damaging yin, and nourishes without stagnating pathogens. The various components work together, so that the traditional Chinese medicine composition of the present invention has the effects of both tonifying and purging, and together play the effects of invigorating qi and nourishing yin, promoting blood circulation and unblocking meridians, clearing heat and promoting fluid production.

[0027] On the other hand, Figure 1 As shown, in some embodiments of the present application, a method for preparing a Chinese medicine composition comprises the following preparation steps: The astragalus root, dried dendrobium, kudzu root, notoginseng powder, salvia miltiorrhiza, coptis root slices and bamboo leaves are pre-treated and set aside; adding the pretreated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves into water for soaking, then directly boiling the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein for a first time, and filtering to obtain a first decoction and medicinal residues; Adding water to the medicinal residue for a second decoction, adding Panax notoginseng powder during the decoction, and filtering to obtain a second decoction; The first decoction and the second decoction are mixed and concentrated to obtain the traditional Chinese medicine composition.

[0028] Specifically, the water used in the preparation method is preferably pure water.

[0029] Specifically, the filtration is performed using a 120-mesh filter cloth.

[0030] It can be understood that the present invention significantly improves the dissolution rate of water-soluble core components such as astragaloside IV and dendrobium polysaccharide through the soaking process, and avoids the continuous high-temperature degradation of fat-soluble components such as tanshinone IIA through the gradient decoction process.

[0031] In some embodiments of the present application, the pretreatment is as follows: rinsing the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis chinensis slices and bamboo leaves with water twice, each time for 10 seconds, then cutting the salvia miltiorrhiza into 2-3 mm thick slices, and cutting the bamboo leaves into 5-8 cm long segments; The Panax notoginseng powder was passed through an 80-mesh sieve.

[0032] Specifically, when rinsing with water, the water is preferably pure water.

[0033] It is understandable that rinsing Astragalus, dried Dendrobium, Pueraria, Salvia miltiorrhiza, Coptis chinensis slices and Bambusa chinensis leaves with water twice can effectively remove surface dust; cutting Salvia miltiorrhiza into 2-3mm thick slices can increase the contact area and improve the dissolution rate of Tanshinone ⅡA; cutting Bambusa chinensis leaves into 5-8cm long segments can avoid floating during decoction and causing uneven heating; passing Panax notoginseng powder through an 80-mesh sieve can ensure fineness, facilitate subsequent dissolution, and avoid clumping.

[0034] In some embodiments of the present application, the soaking is specifically as follows: adding 10 times the mass of water to the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices and bamboo leaves, and soaking them at room temperature of 20-25°C for 45 minutes.

[0035] In some embodiments of the present application, the first decoction is specifically: heating the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein to boiling, then reducing the heat to maintain a slight boil, and decocting for 60 minutes.

[0036] Specifically, the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices and bamboo leaves are placed in a ceramic decoction pot, and pure water 10 times the total mass of the medicinal materials is added. The mixture is soaked at room temperature (20-25°C) for 45 minutes, and then the ceramic decoction pot is directly heated to boiling, and then the heat is reduced to a low boil and maintained. At this time, the temperature of the medicinal liquid is 95-98°C, and the mixture is decocted for 60 minutes.

[0037] It is understandable that the amount of 10 times the amount of pure water can fully soak all the medicinal materials. Combined with the increased contact area of ​​​​thin slices of Salvia miltiorrhiza and cut sections of light bamboo leaves, it can avoid local drying of the medicinal materials and uneven dissolution of the effective ingredients. At the same time, the ceramic pot material is stable and will not chemically react with the medicinal ingredients, thus ensuring the purity of the ingredients; at the same time, the room temperature environment of 20-25℃ can not only provide suitable conditions for the dissolution of water-soluble core ingredients such as astragalus saponins and dendrobium polysaccharides, avoiding high-temperature soaking to destroy the activity of the ingredients, but also can ensure the full dissolution of water-soluble ingredients through the scientific duration of 45 minutes. While ensuring the full dissolution of water-soluble ingredients, it avoids insufficient dissolution due to too short soaking or too long soaking causing time waste, laying a good foundation for the subsequent gradient decoction link, and effectively reducing the need to extend the high-temperature decoction time due to insufficient water absorption of the medicinal materials in the subsequent decoction process, thereby avoiding the degradation of fat-soluble ingredients such as tanshinone ⅡA, and ultimately helping to improve the overall dissolution rate of effective ingredients.

[0038] In some embodiments of the present application, the second decoction is specifically as follows: adding 8 times the weight of water to the medicinal residue, heating it to boiling over high heat, then turning to low heat, and maintaining a slight boil for 40 minutes.

[0039] In some embodiments of the present application, the Panax notoginseng powder is added 10 minutes before the end of the second decoction and stirred for 5 minutes.

[0040] Specifically, 8 times the weight of pure water was added to the medicinal residue, and the mixture was boiled over high heat, then reduced to low heat and kept slightly boiling. When the mixture was boiled for 30 minutes, the notoginseng powder was evenly sprinkled into the medicinal liquid, stirred for 5 minutes, and continued to boil slightly for 5 minutes. It can be understood that the present invention adopts a gradient decoction process, which can be specifically adapted to the solubility and stability characteristics of different active ingredients. The first long-term low-heat boiling can fully extract water-soluble components such as astragalus saponins and dendrobium polysaccharides, avoiding the problem of insufficient dissolution caused by insufficient time in conventional decoction; the second shorter-time decoction can not only further explore the active ingredients remaining in the medicinal residue, but also avoid the degradation of fat-soluble components such as tanshinone ⅡA and berberine due to excessive high-temperature decoction, thereby ensuring its core effect of improving microcirculation and clearing heat and dampness; at the same time, adding Panax notoginseng powder at the end of the second decoction and only boiling for 5 minutes can effectively avoid the ineffectiveness of Panax notoginseng saponins due to long-term high-temperature hydrolysis, thereby retaining its blood-activating and hemostatic activity to the maximum extent. Overall, this gradient decoction process not only solves the pain points of conventional single-heat decoction, such as "insufficient dissolution of water-soluble components, easy degradation of fat-soluble components, and easy ineffectiveness of heat-sensitive components", thereby improving the total dissolution rate of effective ingredients, but also provides process guarantees for the efficacy of Chinese medicine compositions by scientifically controlling decoction time and temperature.

[0041] In some embodiments of the present application, the concentration is specifically: heating the mixed decoction over low heat to concentrate it.

[0042] Specifically, place the crude decoction in a ceramic pot, keep the temperature of the medicine liquid at 90-95℃ over low heat, and concentrate it to 1 / 5 of the volume. Stir it once every 10 minutes to avoid sticking to the bottom.

[0043] It is understandable that maintaining a temperature range of 90-95°C over low heat can not only provide sufficient heat for concentration to efficiently remove excess water, but also avoid the degradation threshold of fat-soluble components such as tanshinone IIA and berberine, effectively reducing the loss of active ingredients compared to the conventional technical solution of boiling and concentrating at 100°C; concentrating to 1 / 5 of the volume can increase the concentration of active ingredients by 5 times, ensuring that the active ingredients in the dosage reach the required therapeutic threshold and meet the needs of diseases such as type 2 diabetes combined with coronary heart disease for the synergistic therapeutic effect of "tonifying qi and nourishing yin, promoting blood circulation and removing blood stasis", while avoiding excessive concentration leading to bitter taste or gastrointestinal irritation; stirring once every 10 minutes during the process can effectively prevent the liquid from becoming sticky due to uneven local heating, while promoting vertical convection of the liquid, ensuring uniform concentration of ingredients in each region, and avoiding the precipitation of impurities due to excessive local concentration; Example 1 S1. Take 25g of Astragalus, 25g of dried Dendrobium, 25g of Pueraria root, 1g of Panax notoginseng powder, 25g of Salvia miltiorrhiza, 4g of Coptis chinensis slices, and 10g of Bambusa longifolia leaves; S2. Rinse the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices, and bamboo leaves with water twice for 10 seconds each time, then cut the salvia miltiorrhiza into 2-3 mm thick slices and cut the bamboo leaves into 5-8 cm long segments; pass the Panax notoginseng powder through an 80-mesh sieve; S3, placing the treated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices and light bamboo leaf in a ceramic decoction pot, adding pure water 10 times the total mass of the medicinal materials, soaking at room temperature (20-25°C) for 45 minutes, then directly heating the ceramic decoction pot to boiling, turning to low heat and maintaining a slight boil, at which time the medicinal liquid temperature is 95-98°C, decocting for 60 minutes, and filtering with a 120-mesh filter cloth after the end to obtain a first decoction and medicinal residues; S4, add 8 times the mass of pure water to the medicinal residue, boil over high heat and then turn to low heat, keep boiling slightly, and evenly sprinkle the treated Panax notoginseng powder into the medicinal liquid when it is decocted for 30 minutes, stir for 5 minutes, continue to boil slightly for 5 minutes, and filter with a 120-mesh filter cloth to obtain a second filtrate; S5. The first filtrate and the second filtrate are mixed and placed in a ceramic pot. The temperature of the medicinal liquid is maintained at 90-95° C. over low heat and concentrated to 1 / 5 of the volume. The mixture is stirred every 10 minutes to obtain the Chinese medicine composition.

[0044] Example 2 S1. Take 30g of Astragalus, 30g of dried Dendrobium, 30g of Pueraria root, 3g of Panax notoginseng powder, 30g of Salvia miltiorrhiza, 6g of Coptis chinensis slices and 15g of Bambusa longifolia leaves; S2. Rinse the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices, and bamboo leaves with water twice for 10 seconds each time, then cut the salvia miltiorrhiza into 2-3 mm thick slices and cut the bamboo leaves into 5-8 cm long segments; pass the Panax notoginseng powder through an 80-mesh sieve; S3, placing the treated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices and light bamboo leaf in a ceramic decoction pot, adding pure water 10 times the total mass of the medicinal materials, soaking at room temperature (20-25°C) for 45 minutes, then directly heating the ceramic decoction pot to boiling, turning to low heat and maintaining a slight boil, at which time the medicinal liquid temperature is 95-98°C, decocting for 60 minutes, and filtering with a 120-mesh filter cloth after the end to obtain a first decoction and medicinal residues; S4, add 8 times the mass of pure water to the medicinal residue, boil over high heat and then turn to low heat, keep boiling slightly, and evenly sprinkle the treated Panax notoginseng powder into the medicinal liquid when it is decocted for 30 minutes, stir for 5 minutes, continue to boil slightly for 5 minutes, and filter with a 120-mesh filter cloth to obtain a second filtrate; S5. The first filtrate and the second filtrate are mixed and placed in a ceramic pot. The temperature of the medicinal liquid is maintained at 90-95° C. over low heat and concentrated to 1 / 5 of the volume. The mixture is stirred every 10 minutes to obtain the Chinese medicine composition.

[0045] Example 3 S1. Take 35g of Astragalus, 35g of dried Dendrobium, 35g of Pueraria root, 5g of Panax notoginseng powder, 35g of Salvia miltiorrhiza, 8g of Coptis chinensis slices and 20g of Bambusa longifolia leaves; S2. Rinse the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices, and bamboo leaves with water twice for 10 seconds each time, then cut the salvia miltiorrhiza into 2-3 mm thick slices and cut the bamboo leaves into 5-8 cm long segments; pass the Panax notoginseng powder through an 80-mesh sieve; S3, placing the treated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis root slices and light bamboo leaf in a ceramic decoction pot, adding pure water 10 times the total mass of the medicinal materials, soaking at room temperature (20-25°C) for 45 minutes, then directly heating the ceramic decoction pot to boiling, turning to low heat and maintaining a slight boil, at which time the medicinal liquid temperature is 95-98°C, decocting for 60 minutes, and filtering with a 120-mesh filter cloth after the end to obtain a first decoction and medicinal residues; S4, add 8 times the mass of pure water to the medicinal residue, boil over high heat and then turn to low heat, keep boiling slightly, and evenly sprinkle the treated Panax notoginseng powder into the medicinal liquid when it is decocted for 30 minutes, stir for 5 minutes, continue to boil slightly for 5 minutes, and filter with a 120-mesh filter cloth to obtain a second filtrate; S5. The first filtrate and the second filtrate are mixed and placed in a ceramic pot. The temperature of the medicinal liquid is maintained at 90-95° C. over low heat and concentrated to 1 / 5 of the volume. The mixture is stirred every 10 minutes to obtain the Chinese medicine composition.

[0046] Effect test Eight of 56 SD rats were randomly selected as the normal control group (Con group). The remaining 48 rats were fed a high-sugar, high-fat diet combined with streptozotocin (STZ) to induce a T2DM model. The rats with successful modeling were randomly divided into a model group (Mod group), Example 1 group (Qhgq-L group), Example 2 group (Qhgq-H group), and a dapagliflozin group (Dglj group), each with 12 rats. Each group received the corresponding intervention method for 4 weeks. Myocardial injury was induced by injection of isoproterenol (ISO) at the third week of intervention. The general condition of the rats was observed, and glucose and lipid metabolism indicators, including fasting blood glucose (FBG), fasting insulin (FINS), total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL), were measured. The insulin resistance index (HOMA-IR) and insulin secretion index (HOMA-β) were calculated. Echocardiography was used to evaluate the cardiac function of the rats. The serum levels of adenosine triphosphate (ATP), nitric oxide (NO), and endothelin-1 (ET-1) were measured by ELISA. The pathological damage of myocardial and pancreatic tissues was observed by HE staining. The expressions of AMPK, PGC-1α, SIRT1, p-AKT, and GLUT4 proteins in myocardial and pancreatic tissues were detected by Western blot and immunohistochemistry.

[0047] The body weight of mice in each group is as follows Figure 2 And as shown in Table 1: Table 1 Body weight of rats in each group before and after intervention at different time points ( ±s,g)

[0048] Compared with the Con group, *P<0.05, **P<0.01; compared with the Mod group, #P<0.05, ##P<0.01; compared with before intervention, &P<0.05, &&P<0.01.

[0049] The food intake and water intake of mice in each group are shown in Table 2 and Figure 3 As shown: Table 2 24h water intake of rats in each group before and after intervention ( ±s, mL)

[0050] Compared with the Con group, *P<0.05, **P<0.01; compared with the Mod group, #P<0.05, ##P<0.01.

[0051] It can be seen that compared with the model group, the Example 1 and 2 groups significantly improved the abnormal weight gain trend and effectively alleviated the polydipsia symptoms (P<0.01).

[0052] The glucose metabolism and pancreatic islet function of mice in each group are shown in Table 3, Table 4 and Figure 4 As shown: Table 3 FBG of rats in each group at different time points before and after intervention ( ±s, mmol / L)

[0053] Compared with the Con group, *P<0.05, **P<0.01; compared with the Mod group, #P<0.05, ##P<0.01; compared with before intervention, &P<0.05, &&P<0.01.

[0054] Table 4. FINS, HOMA-IR, and HOMA-β levels in rats of each group ( ±s, n=6)

[0055] Compared with the Con group, *P<0.05, **P<0.01; compared with the Mod group, #P<0.05, ##P<0.01.

[0056] Figure 4(a) FBG change trends of rats in each group; (b) comparison of FINS, HOMA-IR, and HOMA-β levels of rats in each group; * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001.

[0057] It can be seen that, as for the glucose metabolism index, FBG of Example 1 and 2 groups was lower than that of the model group after 4 weeks of intervention (P<0.01), but there was no difference compared with before intervention, while FINS level and HOMA-IR were significantly improved (P<0.01).

[0058] The blood lipid levels of mice in each group are shown in Table 5 and Figure 5 shown Table 5. TC, TG, and LDL levels in rats in each group ( ±s, n=6, mmol / L)

[0059] Figure 5 In the table, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001.

[0060] It can be seen that in terms of blood lipids, compared with the model group, the Example 1 and 2 groups had lower TC, TG, and LDL levels.

[0061] The cardiac function of mice in each group is shown in Table 6 and Figure 6 As shown:

[0062] Figure 6 (a) M-mode ultrasound images of the hearts of rats in each group; (b) comparison of LVIDs, LVIDd, CO, LV MassCor, LVAWd, and LVPWd of rats in each group; * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001.

[0063] It can be seen that the echocardiographic results showed that the intervention of the Chinese medicine composition in Examples 1 and 2 significantly increased the left ventricular end-systolic and end-diastolic internal diameters and cardiac output, and reduced the left ventricular mass and ventricular wall thickness.

[0064] HE staining results of mice in each group are shown in Figure 7-8 As shown, the intervention of the Chinese medicine composition in Examples 1 and 2 significantly reduced myocardial fibrosis and necrosis, significantly alleviated interstitial inflammatory cell infiltration and fibrosis, increased islet volume, reduced pancreatic albuterol β cell degeneration / necrosis, and increased their number.

[0065] The Elisa results of each group of mice are shown in Table 7 and Figure 9 As shown:

[0066] Figure 9 In the table, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001.

[0067] It can be seen that the ELISA results show that the intervention of the Chinese medicine composition in Examples 1 and 2 can reduce the levels of NO and ET-1, and the high-dose group significantly increased the ATP level (P<0.01).

[0068] Western blot and immunohistochemistry results of mice in each group are shown in Figure 2. Figure 10 As shown, Figure 10 (a) Electrophoresis and relative expression levels of AMPK and PGC-1α in myocardial tissue of rats in each group; (b) Electrophoresis and relative expression levels of p-AKT, SIRT1, and GLUT4 in myocardial tissue of rats in each group; * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and **** indicates P < 0.0001.

[0069] It can be seen that the Western blot and immunohistochemistry results showed that the expression of AMPK, SIRT1, PGC-1α, p-AKT and GLUT4 proteins in Groups 1 and 2 were significantly upregulated (P<0.01).

[0070] In summary, the Chinese herbal composition of the present invention significantly improved abnormal body weight, polydipsia, glucose and lipid metabolism disorders, insulin resistance, myocardial and pancreatic pathological damage, and cardiac function in T2DM rats. It also upregulated the expression of AMPK, SIRT1, PGC-1α, p-AKT, and GLUT4 proteins, improving energy metabolism and vascular function. By regulating the AMPK / SIRT1 / PGC-1α pathway and improving insulin resistance, the Chinese herbal composition of the present invention may play a role in preventing and treating T2DM combined with CHD.

[0071] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition, characterized in that The composition includes the following parts by weight: 25-35 parts of Astragalus, 25-35 parts of dried Dendrobium, 25-35 parts of Pueraria, 1-5 parts of Panax notoginseng powder, 25-35 parts of Salvia miltiorrhiza, 4-8 parts of Coptis chinensis slices and 10-20 parts of Bambusoideae.

2. A method for preparing the Chinese medicine composition according to claim 1, characterized in that: The method comprises the following preparation steps: The astragalus root, dried dendrobium, kudzu root, notoginseng powder, salvia miltiorrhiza, coptis root slices and bamboo leaves are pre-treated and set aside; adding the pretreated astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves into water for soaking, then directly boiling the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein for a first time, and filtering to obtain a first decoction and medicinal residues; Adding water to the medicinal residue for a second decoction, adding Panax notoginseng powder during the decoction, and filtering to obtain a second decoction; The first decoction and the second decoction are mixed and concentrated to obtain the traditional Chinese medicine composition.

3. The method for preparing the Chinese medicine composition according to claim 2, wherein The pretreatment is as follows: Astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices and bamboo leaves are rinsed with water twice, each time for 10 seconds, then the salvia miltiorrhiza is cut into 2-3 mm thick slices, and the bamboo leaves are cut into 5-8 cm long segments; The Panax notoginseng powder was passed through an 80-mesh sieve.

4. The method for preparing the Chinese medicine composition according to claim 3, wherein The soaking is specifically as follows: adding 10 times the mass of water to the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices and bamboo leaves, and soaking them at room temperature of 20-25° C. for 45 minutes.

5. The method for preparing the Chinese medicine composition according to claim 4, wherein The first decoction is specifically as follows: heating the soaking liquid and the astragalus, dried dendrobium, kudzu root, salvia miltiorrhiza, coptis slices, and bamboo leaves therein until boiling, then turning to low heat, maintaining a slight boil, and decocting for 60 minutes.

6. The method for preparing the Chinese medicine composition according to claim 5, wherein: The second decoction is specifically as follows: adding 8 times the weight of water to the medicinal residue, heating over high heat until boiling, then turning to low heat, and keeping it at a slight boil for 40 minutes.

7. The method for preparing the Chinese medicine composition according to claim 6, wherein: Add the Panax notoginseng powder 10 minutes before the end of the second decoction and stir for 5 minutes.

8. The method for preparing the Chinese medicine composition according to claim 7, wherein: The concentration is specifically as follows: heating the mixed decoction over low heat to concentrate it.

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