Traditional Chinese medicine composition for preventing and treating gonitis and preparation method thereof

Abstract

The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 to 25 parts of radix salviae miltiorrhizae, 8 to 15 parts of rhizoma chuanxiong, 12 to 20 parts of radix angelicae sinensis, 7 to 15 parts of herba lycopi, 15 to 30 parts of poria cocos, 8 to 15 parts of human placenta, 18 to 30 parts of herba speranskiae tuberculatae, 15 to 25 parts of caulis spatholobi, 25 to 40 parts of radix paeoniae alba, 12 to 20 parts of radix rehmanniae and 8 to 15 parts of liquorice root. The traditional Chinese medicine composition takes salvia miltiorrhiza, ligusticum wallichii, angelica sinensis, herba lycopi, poria cocos, human placenta, garden balsam stem, caulis spatholobi, radix paeoniae alba, radix rehmanniae and liquorice as raw materials, follows the medication principle of monarch, minister, assistant and guide of traditional Chinese medicines, can tonify the liver and kidney and promote blood circulation to remove blood stasis by combining the medicines, and has a good treatment effect on liver and kidney deficiency and qi stagnation and blood stasis type knee arthralgia.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, and more particularly to a traditional Chinese medicine composition for preventing and treating knee arthritis and a preparation method thereof. BACKGROUND

[0002] Knee arthritis is a disabling disease with increasing incidence and prevalence in the general population. As one of the most common orthopedic diseases, knee arthritis not only brings pain to patients, but also causes great pressure on the health care system and the social economy.

[0003] The pathogenesis of knee arthritis is complex, and its specific pathogenesis has not been clearly defined. Currently, there is still no effective method to block the disease development process of knee arthritis. With the aging of the population, the various pressures brought about by knee arthritis will increase, and the intervention of traditional Chinese medicine compound in knee arthritis has the advantages of multiple targets, rich action mechanism and low cost and high efficiency. Therefore, exploring an effective method to intervene in knee arthritis is an important problem to be solved at present. SUMMARY

[0004] Based on the above technical problems existing in the prior art, the purpose of the present application is to provide a traditional Chinese medicine composition for preventing and treating knee arthritis. The traditional Chinese medicine composition can tonify liver and kidney, activate blood and resolve stasis, and has good therapeutic effect on knee arthritis of liver and kidney deficiency and blood stasis type.

[0005] In order to achieve the above purpose, the technical scheme of the present application is as follows:

[0006] A traditional Chinese medicine composition for preventing and treating knee arthritis, the raw materials of the active ingredients of which comprise, by weight fraction:

[0007] 15-25 parts of Danshen, 8-15 parts of Chuanxiong, 12-20 parts of Danggui, 7-15 parts of Zelan, 15-30 parts of Fuling, 8-15 parts of Zihewan, 18-30 parts of Tougucao, 15-25 parts of Jixueteng, 25-40 parts of Baishao, 12-20 parts of Shengdi and 8-15 parts of Gancao.

[0008] In some embodiments, the raw materials of the active ingredients of the traditional Chinese medicine composition comprise, by weight fraction:

[0009] 15-25 parts of Danshen, 8-15 parts of Chuanxiong, 12-20 parts of Danggui, 7-15 parts of Zelan, 15-30 parts of Fuling, 8-15 parts of Zihewan, 18-30 parts of Tougucao, 15-25 parts of Jixueteng, 25-40 parts of Baishao, 12-20 parts of Shengdi and 8-15 parts of Gancao.

[0010] In some embodiments, the raw materials of the active ingredients of the traditional Chinese medicine composition comprise, by weight fraction:

[0011] Salvia miltiorrhiza 20 parts, Chuanxiong 10 parts, Angelica 15 parts, Lonicera 10 parts, Poria 20 parts, Shudihuang 10 parts, Radix Sophorae Tonkinensis 20 parts, Caulis Spatholobi 20 parts, Radix Paeoniae Alba 30 parts, Radix Rehmanniae 15 parts and Radix Glycyrrhizae 10 parts.

[0012] In some embodiments, the raw materials of the active ingredients of the traditional Chinese medicine composition consist of the following raw materials by weight fraction:

[0013] Salvia miltiorrhiza 20 parts, Chuanxiong 10 parts, Angelica 15 parts, Lonicera 10 parts, Poria 20 parts, Shudihuang 10 parts, Radix Sophorae Tonkinensis 20 parts, Caulis Spatholobi 20 parts, Radix Paeoniae Alba 30 parts, Radix Rehmanniae 15 parts and Radix Glycyrrhizae 10 parts.

[0014] In some embodiments, the traditional Chinese medicine composition is a preparation prepared from the powder of the raw drug, the water or organic solvent extract of the raw drug as the active ingredient, and the pharmaceutically acceptable adjuvant.

[0015] In some embodiments, the traditional Chinese medicine composition is a granule, a pill, a tablet, a decoction or a hard capsule.

[0016] The application also provides a preparation method of the traditional Chinese medicine composition for preventing and treating knee arthritis according to any one of the above embodiments, which comprises the following steps:

[0017] Salvia miltiorrhiza, Chuanxiong, Angelica, Lonicera, Poria, Shudihuang, Radix Sophorae Tonkinensis, Caulis Spatholobi, Radix Paeoniae Alba, Radix Rehmanniae and Radix Glycyrrhizae are weighed and mixed, water is added and decocted twice, the decoction is concentrated, and the temperature is cooled to room temperature to obtain the traditional Chinese medicine composition.

[0018] In some embodiments, Salvia miltiorrhiza, Chuanxiong, Angelica, Lonicera, Poria, Shudihuang, Radix Sophorae Tonkinensis, Caulis Spatholobi, Radix Paeoniae Alba, Radix Rehmanniae and Radix Glycyrrhizae are weighed and mixed, 10-15 times the amount of water is added and decocted for 1-2 hours, 8-10 times the amount of water is added and decocted for 1-1.5 hours.

[0019] In some embodiments, the decoction is concentrated to a relative density of 1.25-1.30 at 50-60℃ to obtain an extract, and the pharmaceutically acceptable adjuvant is added to the extract, mixed and prepared into the required preparation.

[0020] The pharmacological effects of the application are as follows:

[0021] Salvia miltiorrhiza: bitter, slightly cold; heart and liver meridians. Activating blood and removing blood stasis, dredging collaterals and relieving pain, clearing heart and relieving restlessness. Used for chest pain, irregular menstruation, accumulation of masses, sore and swollen sores, etc.

[0022] Chuanxiong: pungent, warm; liver, gallbladder and pericardium meridians. Activating blood and promoting qi, dispelling wind and relieving pain. Used for blood stasis and qi stagnation, headache, rheumatic arthralgia.

[0023] Angelica sinensis: Sweet, pungent, and warm in nature; it enters the Liver, Heart, and Spleen meridians. It nourishes and invigorates blood circulation, regulates menstruation and relieves pain, and moistens the intestines and promotes bowel movements. It is used for sallow complexion due to blood deficiency, irregular menstruation, abdominal pain due to deficiency-cold, and constipation caused by dry intestines.

[0024] Ze Lan: Bitter, pungent, and slightly warm; enters the Liver and Spleen meridians. It promotes blood circulation and regulates menstruation, dispels blood stasis and resolves carbuncles, and promotes diuresis and reduces swelling. It is used to treat irregular menstruation, traumatic injuries, sores, carbuncles, swelling, and edema.

[0025] Poria: Sweet, bland, and neutral in nature; enters the Heart, Lung, Spleen, and Kidney meridians. It promotes diuresis and eliminates dampness, strengthens the spleen, and calms the mind. It is used for edema, oliguria, phlegm and fluid retention, dizziness and palpitations, spleen deficiency, poor appetite, and restlessness.

[0026] Placenta: Sweet and salty in flavor, warm in nature; enters the Lung, Liver, and Kidney meridians. It replenishes Qi and blood, strengthens essence and tonifies the kidneys. It is used for consumptive emaciation, infertility, low milk production, impotence and spermatorrhea, and asthma due to lung and kidney deficiency.

[0027] Herba Poriae Radix: Pungent, warm in nature; enters the Liver Meridian. It dispels wind and dampness, relaxes tendons and activates collaterals, disperses blood stasis, and relieves pain. It is used for rheumatic pain, muscle and bone cramps, and swelling and pain from falls.

[0028] Millettia reticulata: Bitter and sweet in flavor, warm in nature; enters the Liver and Kidney meridians. It nourishes and invigorates blood circulation, relaxes tendons and activates collaterals. It is used for sallow complexion due to blood deficiency, irregular menstruation, numbness of limbs, and rheumatic pain.

[0029] White Peony Root: Bitter, sour, and slightly cold; enters the Liver and Spleen meridians. It nourishes blood and regulates menstruation, softens the liver and relieves pain, and astringes yin and stops sweating. It is used for menstrual irregularities due to blood deficiency, flank pain, abdominal pain, and spontaneous sweating.

[0030] Shengdi (Shengdi): Sweet and bitter in flavor, cold in nature; enters the Heart, Liver, and Kidney meridians. Clears heat and cools blood, nourishes Yin and promotes fluid production. Used for heat invading the blood, yin deficiency and internal heat, bone steaming and fatigue fever, and thirst due to loss of fluid.

[0031] Licorice: Sweet and neutral in nature, it enters the Heart, Lung, Spleen, and Stomach meridians. It tonifies the spleen and replenishes Qi, clears heat and detoxifies, eliminates phlegm and relieves coughs, and harmonizes various other herbs. It is used for spleen and stomach deficiency, cough with excessive phlegm, and carbuncles, sores, and ulcers.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The traditional Chinese medicine composition provided in the application takes Danshen, Chuanqiong, Danggui, Zelan, Fuling, Zihewan, Tougucao, Jixueteng, Baishao, Shengdi and Gancao as raw materials, and follows the medication principle of traditional Chinese medicine "jun, chen and zhe". Danshen is used as the monarch in the prescription, which can dispel blood stasis, stop pain, clear heat and remove blood stasis, dredge the joints, treat arthralgia, dredge the collaterals and reduce the swelling of knees. Chuanqiong and Zelan can promote blood circulation and relieve pain, and activate blood and promote diuresis, which can help the monarch to enhance the functions of removing blood stasis and promoting diuresis. Danggui can tonify blood and activate blood, and also can remove blood stasis and tonify deficiency, which is consistent with the theory of "long-term arthralgia is caused by deficiency". Fuling can tonify the spleen and remove dampness, which can remove arthralgia caused by dampness. Tougucao can dispel wind and remove dampness, which is good at treating arthralgia of muscles and bones. Jixueteng can nourish blood and dredge collaterals, which is as important as tonifying blood and dredging arthralgia. Baishao can soothe the liver and relieve urgency. Shengdi and Zihewan can tonify the liver and kidney and strengthen the tendons and bones, which can treat the degeneration of knee bones. Gancao can harmonize all the medicines, relieve pain, and also can relieve convulsions together with Baishao. The combination of all the medicines can dredge collaterals without damaging normal body, tonify deficiency without causing blood stasis, strengthen the tendons and bones, and remove arthralgia. DETAILED DESCRIPTION

[0034] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, it will be apparent to one skilled in the art that the present application can be practiced without the specific details presented, that in some instances, well-known methodologies have not been described in detail in order to avoid obscuring the present application. Therefore, the present application is not intended to be limited by the specific embodiments disclosed below, which can be considered as examples of the present application.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for describing particular embodiments only and is not intended to be limiting of the application.

[0036] Example 1

[0037] A traditional Chinese medicine composition for treating osteoarthritis, the active ingredients of which are made of the following raw materials:

[0038] Danshen 20g, Chuanqiong 10g, Danggui 15g, Zelan 10g, Fuling 20g, Zihewan 10g, Tougucao 20g, Jixueteng 20g, Baishao 30g, Shengdi 15g, Gancao 10g;

[0039] The preparation method of the traditional Chinese medicine composition of the present embodiment comprises the following steps:

[0040] S1, Danshen, Chuanqiong, Danggui, Zelan, Fuling, Zihewan, Tougucao, Jixueteng, Baishao, Shengdi and Gancao are weighed according to the raw material ratio, and then 10 times the weight of distilled water is added for decoction twice, each time for 1 hour, and the filtrate is filtered and combined;

[0041] S2, the filtrate is concentrated to 50-60℃ relative density of 1.25-1.30, and then cooled to room temperature to obtain the extract;

[0042] S3, the extract is further dried to obtain the dry extract.

[0043] S4, the extract dry extract powder is crushed to fine powder, and is passed through a 100 mesh sieve to obtain dry extract powder; the dry extract powder and lactose are placed in a fluidized chamber of a fluidized bed, and the air volume is adjusted to make the material boil;

[0044] S5, after preheating the material, the spray is started, and when the temperature of the material reaches 40°C, the atomizer is turned on, the atomizer pressure is adjusted to 0.1 MPa, and then the binder is sprayed into the fluidized chamber to perform granulation; wherein the binder is HPMC with a concentration of 5%;

[0045] S6, the fan speed is adjusted to 1700 rpm, the inlet air temperature is 65°C, the outlet air temperature is 40°C, and the frequency of the peristaltic pump feeding is 8 Hz;

[0046] S8, after the granules are formed, hot air at 50°C is used to continue drying, after 0.5 h, the granules are taken out and weighed, the granules are passed through a No. 1 sieve and a No. 5 sieve respectively, and finally the granules that can pass through the No. 1 sieve but cannot pass through the No. 5 sieve are collected and packaged to obtain granules.

[0047] Example 2

[0048] A traditional Chinese medicine composition for treating osteoarthritis, the active ingredients of which are made of the following raw medicinal materials by weight:

[0049] Salvia miltiorrhiza 20g, Chuanxiong 8g, Angelica 15g, Lamiophlomis 8g, Poria cocos 20g, Purple river car 10g, 20g, 20g, 30g, 15g, 10g;

[0050] The preparation method of the traditional Chinese medicine composition of this example is the same as that of Example 1.

[0051] Example 3

[0052] A traditional Chinese medicine composition for treating osteoarthritis, the active ingredients of which are made of the following raw medicinal materials by weight:

[0053] Salvia miltiorrhiza 20g, Chuanxiong 10g, Angelica 13g, Lamiophlomis 10g, Poria cocos 18g, Purple river car 10g, 20g, 20g, 30g, 15g, 10g;

[0054] The preparation method of the traditional Chinese medicine composition of this example is the same as that of Example 1.

[0055] Example 4

[0056] A traditional Chinese medicine composition for treating osteoarthritis, the active ingredients of which are made of the following raw medicinal materials by weight:

[0057] Radix Salviae Miltiorrhizae 20 g, Ligusticum Chuanxiong 10 g, Angelica 15 g, Lycopi 10 g, Poria 18 g, Purple Cow 8 g, Radix Rubi 18 g, Caulis Spatholobi 20 g, Radix Paeoniae Alba 28 g, Radix Rehmanniae 15 g, Radix Glycyrrhizae 10 g;

[0058] The preparation method of the traditional Chinese medicine composition of the present embodiment is the same as that of Embodiment 1.

[0059] Pharmacological experiment

[0060] In order to further evaluate the curative effect of the traditional Chinese medicine composition of the present application, the pharmacological experiment was carried out on the granules of Embodiment 1, as follows:

[0061] I. Animal experiment

[0062] 1. Experimental animals: 50 healthy male SD rats, SPF level, body weight 200-300 g, purchased from Hunan Academy of Chinese Medicine Animal Experiment Center (Animal Qualification Certificate No.: SCXK(Xiang)2019-0004), free drinking water and food.

[0063] 2. Animal grouping and establishment of knee osteoarthritis rat model

[0064] The rats were randomly divided into 5 groups, 10 rats in each group, namely normal group, model group, sample group, traditional Chinese medicine control group and positive drug group. After 1 week of adaptive feeding, except for the normal group, the rest of the experimental rats were prepared into knee osteoarthritis model rats by modified Hulth method, specifically:

[0065] Anesthetized by intraperitoneal injection of sodium pentobarbital, after anesthesia, the rats were fixed; the right knee joint was taken, the upper end was close to the hip joint, and the lower end was close to the ankle joint, the local hair was removed, the skin was prepared, disinfected, and sterile gauze was laid; the medial meniscus was exposed and removed by medial approach, the anterior cruciate ligament was cut, the anterior drawer test was performed, and the positive result indicated that the anterior cruciate ligament was cut; after washing the joint cavity with normal saline, it was sutured layer by layer, and the wound was dressed with sterile dressing without fixation. Pay attention to not damage the articular cartilage surface during operation, stop bleeding completely, and operate strictly aseptically.

[0066] After the operation, all the rats were intramuscularly injected with penicillin 8000 U per rat for 3 consecutive days.

[0067] 3. Administration method

[0068] After modeling for 4 weeks, the administration was started, and the specific administration method was as follows:

[0069] Normal group: intragastrically administered with the same volume of distilled water, once a day, for 4 consecutive weeks;

[0070] Model group: intragastrically administered with the same volume of distilled water, once a day, for 4 consecutive weeks;

[0071] Sample group: Granules (i.e. granules prepared in Example 1) were administered at a dose of 18.75 g / kg of crude drug concentration by gavage once a day for 4 consecutive weeks.

[0072] Traditional Chinese medicine control group: Jiannibi Decoction (traditional Chinese medicine decoction ingredients: Notopterygium 9 g, turmeric 9 g, angelica 9 g, astragalus 9 g, red peony root 9 g, siler 9 g, licorice 3 g) in Yang's Family Prescriptions was administered by gavage at a dose of 4.06 g / kg of crude drug concentration decoction once a day for 4 consecutive weeks.

[0073] Positive drug group: Celecoxib was administered at a dose of 20 mg / kg by gavage once a day for 4 consecutive weeks.

[0074] 4. Observation index

[0075] The effects of each group on the body weight, serum inflammatory factors, and articular cartilage of the osteoarthritis model rats were observed.

[0076] After 4 weeks of gavage, about 5 mL of blood was collected from the heart using a blood collection tube containing no additives, and allowed to stand at room temperature for 2 h. After the blood coagulated, it was centrifuged (4000 r / min, 5 min) to obtain the supernatant, which was then aspirated and aliquoted into EP tubes for storage at -80°C. The expression of inflammatory factors was monitored by ELISA. After anesthesia and sacrifice, the articular cartilage tissue was collected, and the knee joint of the rat was fixed in 4% paraformaldehyde solution. After decalcification, paraffin embedding, and sectioning, the cartilage and synovial membrane tissue was routinely stained using HE and safranin-fast green staining. After dehydration and mounting, the pathological changes of the cartilage and synovial membrane tissue were observed under an optical microscope. Based on the histological characteristics of the cartilage such as wear, cracking, and peeling, the OARSI scoring system was used to score the cartilage.

[0077] 5. Experimental results

[0078] (1) Effects of each group on the body weight of the osteoarthritis model rats

[0079] After 4 weeks of administration, the body weight of the sample group was significantly higher than that of the traditional Chinese medicine control group and the positive drug control group (p<0.05), as shown in Table 1.

[0080] Table 1 Comparison of body weight of rats in each group before and after administration g)

[0081]

[0082] Note: Compared with the normal group, * p<0.05; compared with the model group, # p<0.05; compared with the traditional Chinese medicine control group, △ p<0.05; compared with the positive drug group, & p<0.05.

[0083] (2) The effect of the sample on serum inflammatory factors in the rat model

[0084] The expression levels of IL-1β and TNF-α in the blood of each group were detected by ELISA, and the results showed that, compared with the normal group, the expression levels of IL-1β and TNF-α in the serum of the remaining four groups were significantly increased (p<0.05); compared with the model group, the contents of IL-6 and TNF-α in the serum of the sample group, the traditional Chinese medicine control group and the positive drug group were significantly reduced (p<0.05); compared with the traditional Chinese medicine control group and the positive drug group, the expression levels of IL-1β and TNF-α in the serum of the sample group were reduced (p<0.05), as shown in Table 2.

[0085] Table 2 Comparison of the contents of IL-1β and TNF-α in the serum of rats in each group ng / L)

[0086]

[0087] Note: compared with the normal group, * p<0.05; compared with the model group, # p<0.05; compared with the traditional Chinese medicine control group, △ p<0.05; compared with the positive drug group, & p<0.05.

[0088] (3) HE, picro-sirius red staining OARSI score results,

[0089] Compared with the normal group, the OARSI scores of the remaining four groups were significantly increased (p<0.05); compared with the model group, the OARSI scores of the sample group, the traditional Chinese medicine control group and the positive drug group were significantly reduced (p<0.05); compared with the traditional Chinese medicine control group, the OARSI score of the sample group was significantly reduced (p<0.05); as shown in Table 3.

[0090]

[0091] Table 3 Comparison of the OARSI scores of the knee joints of rats in each group points

[0092]

[0093] Note: compared with the normal group, * p<0.05; compared with the model group, # p<0.05; compared with the traditional Chinese medicine control group, △ p<0.05.

[0094] II. Clinical research

[0095] 1. Grouping

[0096] ​Treatment group: granules prepared in Example 1, taken orally, 10 g each time, 3 times a day;

[0097] Traditional Chinese medicine control group: Xu Bi Decoction (composition: Notopterygium 9 g, turmeric 9 g, Angelica 9 g, Astragalus 9 g, Red Peony 9 g, Saposhnikovia 9 g, Licorice 3 g) in Yang's Family Prescriptions was given; one dose per day, taken in the morning and evening;

[0098] Positive drug group: etoricoxib tablets 60 mg, once a day.

[0099] 2. Randomized grouping of patients

[0100] From 500 patients with osteoarthritis in the orthopedic clinic, 90 patients were randomly selected, divided into treatment group, traditional Chinese medicine control group and positive drug group at a ratio of 1:1:1, 30 cases in each group; Among them: 10 males and 20 females in the treatment group, aged 60.4±3.6; 10 males and 20 females in the traditional Chinese medicine control group, aged 62.2±3.8; 10 males and 20 females in the positive drug group, aged 61.3±3.7.

[0101] There was no statistically significant difference in the comparison of general data of patients in the three groups before treatment (P>0.05), which was comparable.

[0102] 3. Observation index and efficacy evaluation

[0103] After 2 weeks of medication, the efficacy and adverse reaction rates of the three groups were observed.

[0104] 4. Results

[0105] (1) Comparison of VAS pain scores: There was no statistically significant difference in VAS scores among the three groups before treatment (P>0.05); VAS scores of the three groups were significantly reduced after treatment (P<0.05); Compared with the traditional Chinese medicine control group, the VAS score of the treatment group was significantly reduced (P<0.05); as shown in Table 4.

[0106] Table 4 Comparison of VAS scores of the three groups

[0107]

[0108] Note: Compared with before treatment, * P<0.05; compared with the traditional Chinese medicine control group, # P<0.05.

[0109] (2) Comparison of WOMAC scores: Compared with before treatment, WOMAC scores of the three groups were significantly reduced after treatment (P<0.05); Compared among the three groups after treatment, the WOMAC score of the treatment group was lower than that of the traditional Chinese medicine control group and the positive drug group, and the difference was statistically significant (P<0.05); as shown in Table 5.​

[0110] Table 5 Comparison of three groups of WOMAC scores Points

[0111]

[0112] Note: Compared with the traditional Chinese medicine control group, * P < 0.05; compared with the positive drug group, # P < 0.05.

[0113] (3) Comparison of curative effects: the effective rate of disease treatment in the treatment group was better than that in the traditional Chinese medicine control group and the positive drug group, and the difference was statistically significant (P < 0.05); as shown in Table 6.

[0114] Table 6 Comparison of disease curative effects of three groups

[0115]

[0116] Note: Compared with the traditional Chinese medicine control group, * P < 0.05; compared with the positive drug group, # P < 0.05

[0117] (4) Safety comparison: one patient in the positive drug group had mild stomach discomfort after taking the medicine, which improved after rest, and no adverse reaction events occurred in the other two groups, indicating that the treatment has good safety.

[0118] The technical features of the above-described embodiments can be combined arbitrarily, and to make the description concise, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combinations of the technical features do not exist contradictory, it should be considered as the scope of the present application.

[0119] The above-described embodiments only express several embodiments of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the patent. It should be noted that for ordinary skilled in the art, without departing from the concept of the present application, a number of variations and improvements can be made, which are within the scope of the present application. Therefore, the scope of protection of the present application should be subject to the appended claims.

Claims

1. A Chinese medicine composition for preventing and treating knee arthritis, characterized in that: The raw materials of the active ingredients thereof are calculated by weight and include: 15-25 parts of Salvia miltiorrhiza, 8-15 parts of Chuanxiong, 12-20 parts of Angelica sinensis, 7-15 parts of Zedoariae, 15-30 parts of Poria, 8-15 parts of Placenta, 18-30 parts of Radix Spatholobi, 15-25 parts of Millettia reticulata, 25-40 parts of White Peony Root, 12-20 parts of Rehmannia glutinosa, and 8-15 parts of Licorice.

2. The Chinese medicine composition for preventing and treating knee arthritis according to claim 1, characterized in that: The raw materials of the active ingredients are composed of the following raw materials in parts by weight: 15-25 parts of Salvia miltiorrhiza, 8-15 parts of Chuanxiong, 12-20 parts of Angelica sinensis, 7-15 parts of Zedoariae, 15-30 parts of Poria, 8-15 parts of Placenta, 18-30 parts of Radix Spatholobi, 15-25 parts of Millettia reticulata, 25-40 parts of White Peony Root, 12-20 parts of Rehmannia glutinosa, and 8-15 parts of Licorice.

3. The Chinese medicine composition for preventing and treating knee arthritis according to claim 1, characterized in that: The raw materials of the active ingredients thereof are calculated by weight and include: 20 parts of Salvia miltiorrhiza, 10 parts of Chuanxiong, 15 parts of Chinese Angelica, 10 parts of Herba Eupatorii, 20 parts of Poria, 10 parts of Placenta, 20 parts of Herba Spatholobi, 20 parts of Millettia reticulata, 30 parts of White Peony Root, 15 parts of Radix Rehmanniae and 10 parts of Radix Glycyrrhizae.

4. The Chinese medicine composition for preventing knee arthritis according to claim 3, characterized in that: The raw materials of the active ingredients are composed of the following raw materials in parts by weight: 20 parts of Salvia miltiorrhiza, 10 parts of Chuanxiong, 15 parts of Chinese Angelica, 10 parts of Herba Eupatorii, 20 parts of Poria, 10 parts of Placenta, 20 parts of Herba Spatholobi, 20 parts of Millettia reticulata, 30 parts of White Peony Root, 15 parts of Radix Rehmanniae and 10 parts of Radix Glycyrrhizae.

5. The Chinese medicine composition for preventing and treating knee arthritis according to any one of claims 1 to 4, characterized in that: The traditional Chinese medicine composition is a preparation prepared by adding pharmaceutically acceptable excipients into the powder of the raw material medicine and the water or organic solvent extract of the raw material medicine in the above weight ratio as active ingredients.

6. The Chinese medicine composition for preventing and treating knee arthritis according to any one of claims 1 to 4, characterized in that: The Chinese medicine composition is in the form of granules, pills, tablets, decoctions or hard capsules.

7. The method for preparing the traditional Chinese medicine composition for preventing and treating knee arthritis according to any one of claims 1 to 6, characterized in that: The following steps are involved: Salvia miltiorrhiza, Chuanxiong, Chinese angelica, Zedoariae rhizome, Poria cocos, Placenta centifolia, Herba spatholobi, Millettia reticulatae, Paeonia lactiflora, Rehmannia glutinosa and Licorice are weighed separately, combined, decocted twice with water, the two decoctions are combined, concentrated and cooled to room temperature to obtain the traditional Chinese medicine composition.

8. The method for preparing the Chinese medicine composition for preventing and treating knee arthritis according to claim 7, characterized in that: Weigh salvia miltiorrhiza, chuanxiong, angelica, zedoaria, poria, placenta, scutellaria baicalensis, millettia reticulata, white peony root, raw rehmannia and liquorice respectively, add water and boil twice, add 10-15 times the amount of water for the first time and boil for 1-2 hours; add 8-10 times the amount of water for the second time and boil for 1-1.5 hours.

9. The method for preparing the Chinese medicine composition for preventing and treating knee arthritis according to claim 7, characterized in that: The decoction is concentrated to a relative density of 1.25-1.30 at 50-60° C. to obtain an extract; and pharmaceutically acceptable excipients are added to the extract, mixed evenly, to prepare the desired preparation.

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