Portable solanaceous fruit pollen transportation device and using method

By designing a portable Solanaceae pollen transportation device and using a liquid nitrogen tank for cooling and a sealing mechanism, the temperature control and contamination problems in pollen transportation were solved, and efficient pollen transportation and hybrid pollination were achieved.

CN120793379APending Publication Date: 2025-10-17HANGZHOU ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511159839.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-19
Publication Date
2025-10-17

AI Technical Summary

Technical Problem

During the transportation of Solanaceae pollen, existing technologies make it difficult to effectively maintain a storage temperature of 12°C, resulting in pollen inactivation. The pollen is also easily contaminated during transportation, affecting the success rate of hybrid pollination and genetic purity.

Method used

A portable Solanaceae pollen transport device was designed. It adopted a cooling mechanism and a sealing mechanism. A liquid nitrogen tank was used as a cooling source to maintain a temperature of 12°C. A sealing cover and a clamping mechanism were used to ensure the independent sealing of the pollen box to prevent contamination.

Benefits of technology

It effectively maintains the physiological activity of pollen cells, ensures the success rate of hybrid pollination, reduces pollen loss, lowers breeding costs, and simplifies the operating process. It is suitable for large-scale operations in large breeding bases.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a portable solanaceous fruit pollen transportation device and a using method, and relates to the technical field of transportation equipment, a clamping mechanism is arranged in a fixed shell, a plurality of bearing discs are arranged in an inner shell, solanaceous fruit male parent pollen is stored in each pollen box, and the solanaceous fruit male parent pollen is stored in each pollen box; a plurality of sealing protruding blocks used for sealing the open ends of the pollen boxes are arranged at the bottom of the sealing cover, driving mechanisms used for driving the sealing cover to be separated from the bearing discs are arranged on the two sides between the cabinet body and the inner shell, and a refrigeration space is formed between every two adjacent bearing discs. And a cooling mechanism for cooling each refrigeration space is arranged at the bottom of one side of the cabinet body. According to the invention, solanaceous male parent pollen is respectively put into the plurality of pollen boxes, the preservation temperature of 12 DEG C is accurately maintained through the cooling mechanism and the temperature sensor in the transportation process, pollen inactivation caused by high temperature is avoided, the physiological activity of pollen cells is maintained, and the hybridization pollination success rate of solanaceous male parent pollen and solanaceous female parent pollen is ensured.
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Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of transportation equipment, in particular to a portable solanaceous pollen transportation device and a use method thereof. BACKGROUND

[0002] In order to facilitate data recording, the male parent and the female parent are managed separately, which is convenient for tracing the variety source and avoiding the contamination of pollen of varieties other than the male parent and the female parent, so as to ensure the genetic purity of the hybrid offspring.

[0003] The solanaceous pollen is classified and planted, and the male parent pollen needs to be transported to the female parent pollen seed production base during hybrid pollination.

[0004] In the transportation process, the temperature of the solanaceous pollen needs to be ensured, so as to avoid the inactivation of the pollen caused by high temperature, and therefore a portable solanaceous pollen transportation device is needed. SUMMARY

[0005] The technical problem to be solved by the present application is to overcome the shortcomings of the prior art, and to provide a portable solanaceous pollen transportation device and a use method thereof, which can maintain a 12 DEG C storage temperature through a cooling mechanism and maintain the physiological activity of pollen cells.

[0006] The technical solution adopted to solve the above technical problem is that a top cover is arranged on the top of the cabinet, cabinet doors are arranged on the side of the cabinet, a fixed shell is arranged outside the cabinet, a clamping mechanism for clamping the cabinet doors and the cabinet is arranged inside the fixed shell, an inner shell is arranged inside the cabinet, a plurality of bearing discs are arranged inside the inner shell, a plurality of mounting holes for mounting pollen boxes are arranged on each bearing disc, solanaceous male parent pollen is respectively stored in each pollen box, sealing covers are slidably connected to the two side plates of the inner shell in the vertical direction, the sealing covers are located above the bearing discs, a plurality of sealing protrusions for sealing the open ends of the pollen boxes are arranged on the bottom of the sealing covers, drive mechanisms for driving the sealing covers and the bearing discs to separate are arranged between the two sides of the cabinet and the inner shell, each two adjacent bearing discs are respectively a refrigeration space, the space between the top cover and the uppermost bearing disc is a top refrigeration space, the space between the lowermost bearing disc and the inner bottom plate of the inner shell is a bottom refrigeration space, a temperature sensor is arranged in each refrigeration space, and a cooling mechanism for cooling each refrigeration space is arranged on the bottom of one side of the cabinet.

[0007] Further, the two side plates of the inner shell are respectively provided with a plurality of clamping protrusions, and the two sides of each bearing disc are respectively provided with clamping grooves clamped with the clamping protrusions.

[0008] Further, the driving mechanism is characterized in that: a plurality of fixed plates are arranged outside the two side plates of the inner shell respectively, each fixed plate is fixedly connected with the two sides of the sealing cover through a second spring respectively, each sealing cover is rotatably installed with a contact wheel at the two bottom sides respectively, a plurality of contact rods are slidably connected outside the two side plates of the inner shell along the horizontal direction, each contact rod is machined with an inclined surface at one end for contacting the contact wheel, a plurality of third connecting rods are rotatably installed outside the cabinet, each third connecting rod is provided with a second rotating wheel at one end, and each third connecting rod is provided with a cam at the other end for contacting the other end of the contact rod.

[0009] Further, the cooling mechanism is characterized in that: a plurality of support bases are arranged at one side of the bottom of the cabinet, each support base is installed with a liquid nitrogen tank respectively, a support plate is arranged at one side of the top of the cabinet, the support plate is provided with a positioning mechanism for positioning the top of the liquid nitrogen tank, each liquid nitrogen tank is stored with liquid nitrogen respectively, each liquid nitrogen tank is provided with a first communication sphere at the bottom outlet end for mutual communication, the outlet end of each first communication sphere is in communication with the inlet end of the pipeline, and the outlet end of each pipeline is in communication with the inside of the corresponding refrigeration space.

[0010] Further, the cooling mechanism is characterized in that: a plurality of support bases are arranged at one side of the bottom of the cabinet, each support base is installed with a liquid nitrogen tank respectively, a support plate is arranged at one side of the top of the cabinet, the support plate is provided with a positioning mechanism for positioning the top of the liquid nitrogen tank, each liquid nitrogen tank is stored with liquid nitrogen respectively, each liquid nitrogen tank is provided with a first communication sphere at the bottom outlet end for mutual communication, the outlet end of each first communication sphere is in communication with the inlet end of the pipeline, and the outlet end of each pipeline is in communication with the inside of the corresponding refrigeration space.

[0011] Further, the positioning mechanism is characterized in that: a plurality of pull rods are slidably connected to the support plate along the vertical direction, each pull rod is provided with a handle at the top end respectively, each pull rod is provided with a top positioning plate for positioning the top side of the liquid nitrogen tank at the bottom end respectively, each pull rod is provided with a first spring, one end of each first spring is fixedly connected with the top positioning plate, and the other end of each first spring is fixedly connected with the bottom of the support plate, and each top positioning plate is provided with a guide rod slidably connected with the support plate along the vertical direction.

[0012] Further, the clamping mechanism is that the first rotating shaft and the second rotating shaft are vertically installed on the fixed shell, the third rotating wheel is arranged at the top end of the first rotating shaft, the first gear is arranged at the bottom end of the first rotating shaft, the second gear is arranged in the middle of the second rotating shaft and is in meshing transmission with the first gear, the second rotating shaft is provided with a plurality of buckling assemblies, the buckling assembly is that the second connecting shaft and the first connecting shaft are horizontally installed on the fixed shell, the clamping cam disc for buckling the cabinet door and the cabinet is arranged at one end of the second connecting shaft, the inclined surface of the clamping cam disc is in abutment and clamping with the inclined surface of the cabinet door, the fourth gear is arranged at the other end of the second connecting shaft, the third gear in meshing transmission with the fourth gear is arranged at one end of the first connecting shaft, the second bevel gear is arranged at the other end of the first connecting shaft, the first bevel gear in meshing transmission with the second bevel gear is arranged on the second rotating shaft, and the rotating directions of the clamping cam discs in the adjacent two buckling assemblies are opposite.

[0013] Further, one side plate of each of the inner shells is processed with a plurality of first ventilation openings, one side plate of the cabinet body is processed with second ventilation openings in communication with the first ventilation openings, the side plate of the cabinet body is provided with a one-way valve mechanism for shielding the second ventilation openings, the one-way valve mechanism is that a plurality of slide rods are slidably connected to the side plate of the cabinet body in the horizontal direction, a third spring is arranged on each slide rod, one end of each third spring is fixedly connected with one end of the slide rod, the other end of each third spring is fixedly connected with the inner wall of the side plate of the cabinet body, the plurality of slide rods are fixedly connected with a connecting plate, and a sealing disc for shielding the second ventilation openings is arranged on the connecting plate.

[0014] A use method of a portable solanaceous pollen transportation device, comprising the following steps:

[0015] S1, pretreatment: respectively placing solanaceous paternal pollen in a plurality of pollen boxes and placing in a 4℃ environment for 2 hours of precooling;

[0016] S2, loading: respectively pushing a plurality of bearing discs into the inner shell, horizontally pushing the clamping grooves on both sides of each bearing disc along the clamping convex plates in the inner shell, clamping the clamping grooves on both sides of the bearing disc with the clamping convex plates, covering the bearing disc with a sealing cover, sealing the open end of the pollen box with the sealing protrusions at the bottom of the sealing cover, closing the cabinet door, clamping the cabinet door and the cabinet with the clamping mechanism, respectively positioning the cooling mechanism at the top with each positioning mechanism, separating the positioning mechanism from the top of the cooling mechanism when replacing the cooling mechanism, cooling each sealed space with each cooling mechanism, and monitoring the temperature in real time with the temperature sensor in each sealed space so that the storage temperature in the inner shell is 12℃, and balancing the internal and external air pressures in the cabinet body with the one-way valve mechanism on the cabinet body;

[0017] S3, the whole cabinet is transported to the solanaceous female pollen base, the cabinet door is opened, the driving mechanism drives the sealing cover to separate from the bearing disc, the bearing disc in the inner shell is pulled out, the solanaceous male pollen in the pollen box is taken out, and the solanaceous male pollen is cross-pollinated with the solanaceous female pollen.

[0018] Further, the method for clamping the cabinet door and the cabinet body by the clamping mechanism in S2 is that the third rotating wheel is manually rotated to drive the first gear on the first rotating shaft to rotate, the first gear is in meshing transmission with the second gear, the second gear drives the second rotating shaft to rotate, and the second rotating shaft drives the plurality of buckling assemblies to rotate to buckle the cabinet door and the cabinet body, the rotating directions of the clamping cam plates in the adjacent two buckling assemblies are opposite, and the cabinet door and the cabinet body are buckled from different directions, wherein the method for buckling the cabinet door and the cabinet body by the buckling assembly is that the second rotating shaft drives the first bevel gear to rotate, the first bevel gear is in meshing transmission with the second bevel gear, the second bevel gear drives the third gear of the first connecting shaft to rotate, the third gear is in meshing transmission with the fourth gear, the fourth gear drives the clamping cam plate to rotate through the second connecting shaft, and the clamping cam plate is rotated to be buckled with the cabinet door and the cabinet body.

[0019] The method for cooling each sealing space by each cooling mechanism in S2 is that the first rotating wheel is manually rotated to drive the first ball valve to rotate through the first connecting rod, the first communication hole on the first ball valve is rotated to be in communication with the outlet end of the liquid nitrogen tank and the inlet end of the pipeline, respectively, the output shaft of the first motor drives the second ball valve to rotate through the second connecting rod, the second communication hole on the second ball valve is rotated to be in communication with the pipeline, and the liquid nitrogen in the liquid nitrogen tank enters the sealing space in sequence through the first communication ball, the first communication hole of the first ball valve, the second communication hole of the second ball valve and the pipeline to cool.

[0020] The method for separating the positioning mechanism and the top of the cooling mechanism in S2 is that the handle is manually pulled to drive the pull rod to move upward along the vertical direction on the supporting plate, the pull rod drives the top positioning plate to move along the vertical direction, the guide rod on the top positioning plate moves along the vertical direction on the supporting plate, and the top positioning plate moves to be separated from the liquid nitrogen tank.

[0021] The method for balancing the internal and external air pressures of the cabinet body by the one-way valve mechanism on the cabinet body in S2 is that the slide rod moves along the horizontal direction on the cabinet body under the action of the third spring, and the gas in the inner shell passes through the first vent and the second vent to control the second vent of the cabinet body to be separated from the sealing disc or the second vent to be covered by the sealing disc, so that the internal and external air pressures of the cabinet body are balanced.

[0022] The method for driving the sealing cover to separate from the bearing disc by the driving mechanism in the S3 is that a second rotating wheel is manually rotated, the second rotating wheel drives the cams at the two ends of the third connecting rod to rotate, each cam moves to abut against the other end of the abutting rod, the inclined surface at one end of the abutting rod moves to abut against the abutting wheel, and the two abutting wheels drive the sealing cover to move in the vertical direction on the two side plates of the inner shell under the action of the second spring, so that the sealing cover is separated from the bearing disc.

[0023] The beneficial effects of the present application are as follows: (1) The present application adopts the method of placing multiple pollen boxes in solanaceous paternal pollen respectively, and accurately maintaining the storage temperature at 12 DEG C through the cooling mechanism and temperature sensor during transportation, thereby avoiding high temperature from causing pollen inactivation, maintaining the physiological activity of pollen cells, and ensuring the success rate of solanaceous paternal pollen and solanaceous maternal pollen cross pollination.

[0024] (2) The clamping mechanism in the present application firmly clamps the cabinet door and the cabinet body, avoids loosening of the cabinet door during transportation, and tightly fits the sealing protrusions at the bottom of the sealing cover with the opening end of the pollen box to form an independent sealed space, thereby preventing dust and moisture from polluting the pollen during transportation, avoiding deterioration of the pollen due to oxidation or moisture absorption, and allowing the temperature sensor to monitor and feedback data in real time, so that temperature control abnormalities can be found in time, pollen loss caused by equipment failure is reduced, and breeding cost is reduced.

[0025] (3) The multiple bearing discs in the present application can load different paternal pollen respectively, one transportation can meet the demand of multiple pollen cross combination, the transportation frequency is reduced, and the present application is suitable for large-scale operation of large-scale breeding bases.

[0026] (4) The present application adopts the method of pushing the bearing disc, clamping the cabinet door, and transporting the cabinet body as a whole to the solanaceous maternal pollen base, opening the cabinet door, driving the sealing cover to separate from the bearing disc by the driving mechanism, drawing out the bearing disc in the inner shell, taking out the solanaceous paternal pollen in the pollen box, and cross pollinating the solanaceous paternal pollen and the solanaceous maternal pollen, thereby reducing the operation complexity, allowing the breeding personnel to quickly take out the pollen for pollination, shortening the pollen exposure time, and ensuring the timeliness of pollination. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 is a structural schematic diagram of an embodiment of the portable solanaceous pollen transportation device.

[0028] Figure 2 is Figure 1 a structural schematic diagram of the opening of the cabinet door.

[0029] Figure 3 is Figure 2 a structural schematic diagram from another angle.

[0030] Figure 4 is a structural schematic diagram of parts on the inner shell.

[0031] Figure 5 is Figure 4 Structure diagram of another angle.

[0032] Figure 6 is

[0033] Figure 7 is

[0034] Figure 8 is

[0035] Figure 9 is

[0036] Figure 10 is

[0037] Figure 11 is Figure 10 Structure diagram of another angle.

[0038] Figure 12 is

[0039] Figure 13 is

[0040] Figure 14 is

[0041] Figure 15 is

[0042] Figure 16 is

[0043] Figure 17 is

[0044] 1. Cabinet door; 2. Top cover; 3. Support plate; 4. Positioning mechanism; 401. Handle; 402. First spring; 403. Guide rod; 404. Pull rod; 405. Top positioning plate; 5. Cooling mechanism; 501. Liquid nitrogen tank; 502. First connecting sphere; 503. First rotating wheel; 504. Support base; 505. Pipeline; 506. Second connecting sphere; 507. First connecting rod; 508. First connecting hole; 509. First ball valve; 510. First motor; 511. Second connecting rod; 512. Second ball valve; 513. Second connecting hole; 6. Driving mechanism; 601. Fixing plate; 602. Second spring; 603. Interference wheel; 604. Interference rod; 605. Second rotating wheel; 606. Cam; 607. Third connecting rod; 7. Fixing Casing; 8. Clamping mechanism; 801. Third rotating wheel; 802. First rotating shaft; 803. Second rotating shaft; 804. First gear; 805. Second gear; 806. First bevel gear; 807. Third gear; 808. First connecting shaft; 809. Clamping cam plate; 810. Second connecting shaft; 811. Fourth gear; 812. Second bevel gear; 9. One-way valve mechanism; 901. Sealing plate; 902. Connecting plate; 903. Sliding rod; 904. Third spring; 10. Sealing strip; 11. Pollen box; 12. Carrying plate; 13. Sealing cover; 14. Temperature sensor; 15. Cabinet; 16. Inner casing; 17. First vent; 18. Clamping protrusion; 19. Clamping groove; 20. Sealing protrusion; 21. Mounting hole; 22. Second vent; 23. Clamping plate. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0046] like Figures 1 to 9 As shown, the portable Solanaceae pollen transport device of this embodiment is composed of a cabinet door 1, a top cover 2, a support plate 3, a positioning mechanism 4, a cooling mechanism 5, a driving mechanism 6, a fixed shell 7, a clamping mechanism 8, a one-way valve mechanism 9, a sealing strip 10, a pollen box 11, a carrying plate 12, a sealing cover 13, a temperature sensor 14, a cabinet 15, an inner shell 16, a first vent 17, a snap-on protrusion 18, a snap-on groove 19, a sealing protrusion 20, a mounting hole 21, a second vent 22, and a clamping plate 23.

[0047] The top cover 2 is arranged on the top of the cabinet body 15, the cabinet door 1 is arranged on the side of the cabinet body 15, the sealing strip 10 is arranged on the inner side of the cabinet door 1 and is in contact with the cabinet body 15, the fixed shell 7 is arranged on the outer side of the cabinet body 15, the clamping mechanism 8 for clamping the cabinet door 1 and the cabinet body 15 is arranged in the fixed shell 7, the inner shell 16 is arranged in the cabinet body 15, the plurality of bearing plates 12 are arranged in the inner shell 16, the plurality of clamping convex plates 18 are arranged on the two side plates of the inner shell 16 respectively, and the clamping grooves 19 for clamping the clamping convex plates 18 are respectively machined on the two sides of each bearing plate 12. The plurality of mounting holes 21 for mounting the pollen boxes 11 are machined on each bearing plate 12, the pollen boxes 11 are respectively arranged in each bearing plate 12, the solanaceous paternal pollen is respectively stored in each pollen box 11, the sealing cover 13 is slidably connected to the two side plates of the inner shell 16 in the vertical direction and is located above the bearing plate 12, the sealing convex blocks 20 for sealing the opening ends of the pollen boxes 11 are arranged on the bottom of the sealing cover 13, the driving mechanism 6 for driving the sealing cover 13 and the bearing plate 12 to separate is arranged between the cabinet body 15 and the inner shell 16 on the two sides, the refrigeration spaces are respectively arranged between every two adjacent bearing plates 12, the space between the top cover 2 and the uppermost bearing plate 12 is the top refrigeration space, the space between the lowermost bearing plate 12 and the inner bottom plate of the inner shell 16 is the bottom refrigeration space, the temperature sensor 14 is arranged in each refrigeration space, and the cooling mechanism 5 for cooling each refrigeration space is arranged on the bottom of one side of the cabinet body 15.

[0048] As shown in Figure 10 , the positioning mechanism 4 is coupled by the handle 401, the first spring 402, the guide rod 403, the pull rod 404 and the top positioning plate 405.

[0049] The positioning mechanism 4 is that the plurality of pull rods 404 are slidably connected to the support plate 3 in the vertical direction, the handle 401 is arranged on the top end of each pull rod 404, the top positioning plate 405 for positioning the top side of the liquid nitrogen tank 501 is arranged on the bottom end of each pull rod 404, the clamping plate 23 for clamping and fixing the liquid nitrogen tank 501 is arranged on the outer side of the cabinet body 15, the first spring 402 is arranged on each pull rod 404, one end of each first spring 402 is fixedly connected with the top positioning plate 405, the other end of each first spring 402 is fixedly connected with the bottom of the support plate 3, and the guide rod 403 slidably connected with the support plate 3 in the vertical direction is arranged on the top of each top positioning plate 405.

[0050] As shown in Figures 10 to 13 , the cooling mechanism 5 is coupled by the liquid nitrogen tank 501, the first communication ball 502, the first rotating wheel 503, the support base 504, the pipeline 505, the second communication ball 506, the first connecting rod 507, the first communication hole 508, the first ball valve 509, the first motor 510, the second connecting rod 511, the second ball valve 512 and the second communication hole 513.

[0051] The cooling mechanism 5 is that a plurality of support bases 504 are arranged on one side of the bottom of the cabinet 15, and a liquid nitrogen tank 501 is arranged on each support base 504; a support plate 3 is arranged on one side of the top of the cabinet 15, and a positioning mechanism 4 for positioning the top of the liquid nitrogen tank 501 is arranged on the support plate 3; liquid nitrogen is stored in each liquid nitrogen tank 501; a first communication sphere 502 is arranged at the outlet end of the bottom of each liquid nitrogen tank 501, and the first communication sphere 502 is in communication with the liquid nitrogen tank 501; the outlet end of each first communication sphere 502 is in communication with the inlet end of a pipeline 505; and the outlet end of each pipeline 505 is in communication with the inside of a corresponding refrigeration space.

[0052] A first connecting rod 507 is rotatably arranged on each first communication sphere 502, one end of each first connecting rod 507 is provided with a first rotating wheel 503, and the other end of each first connecting rod 507 is provided with a first ball valve 509 arranged in the first communication sphere 502; the outer circumferential surface of the first ball valve 509 is in frictional rotation with the inner circumferential surface of the first communication sphere 502; a first communication hole 508 is formed in each first ball valve 509, and the first communication hole 508 is in communication with the outlet end of the liquid nitrogen tank 501 and the inside of the pipeline 505; a second communication sphere 506 is arranged on each pipeline 505 arranged at the bottom of the cabinet 15, and a second ball valve 512 is rotatably arranged in each second communication sphere 506; the outer circumferential surface of each second ball valve 512 is in frictional rotation with the inner circumferential surface of the second communication sphere 506; a second communication hole 513 is formed in each second ball valve 512, and the second communication hole 513 is in communication with the pipeline 505; a second connecting rod 511 is arranged on each second ball valve 512; a plurality of first motors 510 are arranged at the bottom of the cabinet 15; and the output shaft of each first motor 510 is fixedly connected with the second connecting rod 511.

[0053] As shown in Figure 14 The driving mechanism 6 is composed of a fixed plate 601, a second spring 602, an abutting wheel 603, an abutting rod 604, a second rotating wheel 605, a cam 606, and a third connecting rod 607.

[0054] The driving mechanism 6 is that a plurality of fixed plates 601 are arranged on the outer sides of the two side plates of the inner shell 16, each fixed plate 601 is fixedly connected with the two sides of a sealing cover 13 through a second spring 602, an abutting wheel 603 is rotatably arranged on the two bottom sides of each sealing cover 13, a plurality of abutting rods 604 are slidingly connected to the outer sides of the two side plates of the inner shell 16 along the horizontal direction, an inclined surface for abutting with the abutting wheel 603 is formed on one end of each abutting rod 604, a plurality of third connecting rods 607 are rotatably arranged on the outer side of the cabinet 15, a second rotating wheel 605 is arranged on one end of each third connecting rod 607, and a cam 606 is arranged on the other end of each third connecting rod 607 and abuts with the other end of the abutting rod 604.

[0055] As Figures 15 to 16 The clamping mechanism 8 is coupled by the third rotating wheel 801, the first rotating shaft 802, the second rotating shaft 803, the first gear 804, the second gear 805, the first bevel gear 806, the third gear 807, the first connecting shaft 808, the clamping cam disc 809, the second connecting shaft 810, the fourth gear 811 and the second bevel gear 812.

[0056] The clamping mechanism 8 is that the first rotating shaft 802 and the second rotating shaft 803 are vertically installed on the fixed shell 7, the top end of the first rotating shaft 802 is provided with the third rotating wheel 801, the bottom end of the first rotating shaft 802 is provided with the first gear 804, the middle of the second rotating shaft 803 is provided with the second gear 805 which is in meshing transmission with the first gear 804, the second rotating shaft 803 is provided with a plurality of buckling assemblies, the buckling assembly is that the second connecting shaft 810 and the first connecting shaft 808 are horizontally installed on the fixed shell 7, one end of the second connecting shaft 810 is provided with the clamping cam disc 809 which buckles the cabinet door 1 and the cabinet body 15, the inclined surface of the clamping cam disc 809 is in contact with the inclined surface of the cabinet door 1 to clamp, the other end of the second connecting shaft 810 is provided with the fourth gear 811, one end of the first connecting shaft 808 is provided with the third gear 807 which is in meshing transmission with the fourth gear 811, the other end of the first connecting shaft 808 is provided with the second bevel gear 812, the second rotating shaft 803 is provided with the first bevel gear 806 which is in meshing transmission with the second bevel gear 812, the rotating directions of the clamping cam discs 809 in the adjacent two buckling assemblies are opposite.

[0057] As Figure 17 The side plate of the inner shell 16 is processed with a plurality of first ventilation openings 17, the side plate of the cabinet body 15 is processed with the second ventilation openings 22 which are in communication with the first ventilation openings 17, the side plate of the cabinet body 15 is provided with the one-way valve mechanism 9 which is used to shield the second ventilation openings 22, the one-way valve mechanism 9 is coupled by the sealing disc 901, the connecting plate 902, the slide rod 903 and the third spring 904.

[0058] The one-way valve mechanism 9 is that a plurality of slide rods 903 are slidably connected to the side plate of the cabinet body 15 in the horizontal direction, each slide rod 903 is respectively provided with the third spring 904, one end of each third spring 904 is fixedly connected with one end of the slide rod 903, the other end of each third spring 904 is fixedly connected with the inner wall of the side plate of the cabinet body 15, the plurality of slide rods 903 are fixedly connected with the connecting plate 902, the connecting plate 902 is provided with the sealing disc 901 which is used to shield the second ventilation openings 22.

[0059] The use method of the portable solanaceous pollen transportation device of the embodiment includes the following steps:

[0060] S1, respectively put solanaceous paternal pollen in a plurality of pollen boxes 11, and precool in a 4℃ environment for 2 hours;

[0061] S2, respectively push multiple bearing discs 12 into the inside of the inner shell 16, the clamping grooves 19 on both sides of each bearing disc 12 are horizontally pushed into the clamping convex plates 18 inside the inner shell 16, and the clamping grooves 19 on both sides of the bearing disc 12 are clamped with the clamping convex plates 18, the sealing cover 13 is covered above the bearing disc 12, the sealing convex block 20 at the bottom of the sealing cover 13 seals the open end of the pollen box 11, the cabinet door 1 is closed, the clamping mechanism 8 clamps the cabinet door 1 and the cabinet body 15, each positioning mechanism 4 respectively positions the top of the cooling mechanism 5, when the cooling mechanism 5 is replaced, the positioning mechanism 4 is separated from the top of the cooling mechanism 5, each cooling mechanism 5 cools each sealed space, and the temperature sensor 14 in each sealed space is used to monitor the temperature in real time, so that the storage temperature in the inner shell 16 is 12℃, and the one-way valve mechanism 9 on the cabinet body 15 is used to balance the air pressure inside and outside the cabinet body 15, and the cabinet body 15 is transported as a whole to the solanaceous female parent pollen base;

[0062] The method that the clamping mechanism 8 clamps the cabinet door 1 and the cabinet body 15 is that: the third rotating wheel 801 is manually rotated, the first gear 804 on the first rotating shaft 802 is driven to rotate by the third rotating wheel 801, the second gear 805 is driven to rotate by the first gear 804, the second rotating shaft 803 is driven to rotate by the second gear 805, and a plurality of buckling assemblies are driven to rotate to buckle the cabinet door 1 and the cabinet body 15, the rotating directions of the clamping cam plates 809 in the adjacent two buckling assemblies are opposite, and the cabinet door 1 and the cabinet body 15 are buckled from different directions, wherein the method of the buckling assembly is that: the first bevel gear 806 is driven to rotate by the second rotating shaft 803, the second bevel gear 812 is driven to rotate by the first bevel gear 806, the third gear 807 of the first connecting shaft 808 is driven to rotate by the second bevel gear 812, the fourth gear 811 is driven to rotate by the third gear 807, the clamping cam plate 809 is driven to rotate by the fourth gear 811 through the second connecting shaft 810, and the clamping cam plate 809 is rotated to be buckled with the cabinet door 1 and the cabinet body 15;

[0063] The method that each cooling mechanism 5 cools each sealed space is that: the first rotating wheel 503 is manually rotated, the first ball valve 509 is driven to rotate by the first connecting rod 507, the first communication hole 508 on the first ball valve 509 is rotated to be communicated with the outlet end of the liquid nitrogen tank 501 and the inlet end of the pipeline 505 respectively, the output shaft of the first motor 510 is connected with the second ball valve 512 through the second connecting rod 511, the second communication hole 513 on the second ball valve 512 is rotated to be communicated with the pipeline 505, and the liquid nitrogen in the liquid nitrogen tank 501 enters the sealed space in sequence through the first communication ball 502, the first communication hole 508 of the first ball valve 509, the second communication hole 513 of the second ball valve 512 and the pipeline 505 to cool;

[0064] The method for separating the positioning mechanism 4 from the top of the cooling mechanism 5 is that the handle 401 is manually pulled to drive the pull rod 404 to move upward along the vertical direction on the support plate 3, the pull rod 404 drives the top positioning plate 405 to move along the vertical direction, the guide rod 403 on the top positioning plate 405 moves along the vertical direction on the support plate 3, and the top positioning plate 405 moves to be separated from the liquid nitrogen tank 501;

[0065] The method for balancing the air pressure inside and outside the cabinet 15 by the one-way valve mechanism 9 on the cabinet 15 is that the slide rod 903 moves along the horizontal direction on the cabinet 15 under the action of the third spring 904, the gas in the inner shell 16 passes through the first vent 17 and the second vent 22 to control the separation or sealing of the sealing disc 901 from or on the second vent 22 of the cabinet 15, thereby balancing the air pressure inside and outside the cabinet 15;

[0066] S3, open the cabinet door 1, drive the sealing cover 13 to separate from the bearing plate 12 by the driving mechanism 6, pull out the bearing plate 12 in the inner shell 16, take out the solanaceous paternal pollen in the pollen box 11, and cross the solanaceous paternal pollen with solanaceous maternal pollen.

[0067] The method for driving the sealing cover 13 to separate from the bearing plate 12 by the driving mechanism 6 is that the second rotating wheel 605 is manually rotated to drive the cams 606 at both ends of the third connecting rod 607 to rotate, each cam 606 moves to abut against the other end of the abutting rod 604, the inclined surface at one end of the abutting rod 604 moves to abut against the abutting wheel 603, under the action of the second spring 602, the two abutting wheels 603 drive the sealing cover 13 to move along the vertical direction on the two side plates of the inner shell 16, and the sealing cover 13 separates from the bearing plate 12.

[0068] The above only describes the preferred embodiments of the present application and is not used to limit the protection scope of the present application.

Claims

1. A portable Solanaceae pollen transport device, characterized by: A top cover (2) is provided on the top of the cabinet (15), a cabinet door (1) is provided on the side of the cabinet (15), a fixed shell (7) is provided on the outside of the cabinet (15), a clamping mechanism (8) for clamping the cabinet door (1) and the cabinet (15) is provided inside the fixed shell (7), an inner shell (16) is provided inside the cabinet (15), a plurality of supporting plates (12) are provided inside the inner shell (16), a plurality of mounting holes (21) for mounting pollen boxes (11) are processed on each supporting plate (12), and each pollen box (11) stores pollen of the male parent of Solanaceae, and a sealing cover (13) is connected to the two side plates of the inner shell (16) in a vertical sliding direction, and the sealing cover (13) is located above the supporting plate (12). The bottom of the sealing cover (13) is provided with a plurality of sealing protrusions (20) for sealing the open end of the pollen box (11); a driving mechanism (6) for driving the sealing cover (13) to separate from the carrier plate (12) is provided on both sides between the cabinet (15) and the inner shell (16); a refrigeration space is provided between each two adjacent carrier plates (12); the space between the top cover (2) and the uppermost carrier plate (12) is the top refrigeration space; the space between the lowermost carrier plate (12) and the inner bottom plate of the inner shell (16) is the bottom refrigeration space; each refrigeration space is provided with a temperature sensor (14); and a cooling mechanism (5) for cooling each refrigeration space is provided at the bottom of one side of the cabinet (15).

2. The portable Solanaceae pollen transport device according to claim 1, characterized in that: The two side plates of the inner shell (16) are respectively provided with a plurality of snap-fitting protrusions (18), and each carrier plate (12) is respectively processed with snap-fitting grooves (19) on both sides for snapping with the snap-fitting protrusions (18).

3. The portable Solanaceae pollen transport device according to claim 1, characterized in that: The driving mechanism (6) comprises: a plurality of fixed plates (601) are respectively provided on the outer sides of the two side plates of the inner shell (16); each fixed plate (601) is respectively fixedly connected to the two sides of the sealing cover (13) through a second spring (602); a conflict wheel (603) is respectively rotatably installed on the two bottom sides of each sealing cover (13); a plurality of conflict rods (604) are slidably connected to the outer sides of the two side plates of the inner shell (16) along the horizontal direction; one end of each conflict rod (604) is respectively processed with an inclined surface that conflicts with the conflict wheel (603); a plurality of third connecting rods (607) are rotatably installed on the outer side of the cabinet (15); one end of each third connecting rod (607) is provided with a second rotating wheel (605); and both ends of each third connecting rod (607) are respectively provided with a cam (606) that conflicts with the other end of the conflict rod (604).

4. The portable Solanaceae pollen transport device according to claim 1, characterized in that: The cooling mechanism (5) comprises: a plurality of support bases (504) are provided on one side of the bottom of the cabinet (15), a liquid nitrogen tank (501) is installed on each support base (504), a support plate (3) is provided on one side of the top of the cabinet (15), a positioning mechanism (4) for positioning the top of the liquid nitrogen tank (501) is provided on the support plate (3), liquid nitrogen is stored in each liquid nitrogen tank (501), a first communicating sphere (502) is provided at the bottom outlet end of each liquid nitrogen tank (501), the outlet end of each first communicating sphere (502) is communicated with the inlet end of the pipeline (505), and the outlet end of each pipeline (505) is communicated with the inside of the corresponding refrigerated space.

5. The portable Solanaceae pollen transport device according to claim 4, characterized in that: A first connecting rod (507) is rotatably mounted on each of the first connecting rods (502), and a first rotating wheel (503) is provided at one end of each of the first connecting rods (507). A first ball valve (509) located inside the first connecting rod (502) is provided at the other end of each of the first connecting rods (507). The outer circumferential surface of the first ball valve (509) rotates by friction with the inner circumferential surface of the first connecting rod (502). Each of the first ball valves (509) is processed with a first connecting hole (508) that is interconnected with the outlet end of the liquid nitrogen tank (501) and the inside of the pipeline (505). Each pipeline (505) located at the bottom of the cabinet (15) A second connecting sphere (506) is provided on each of the second connecting spheres (506) and is interconnected therewith. A second ball valve (512) is rotatably installed inside each of the second connecting spheres (506). The outer circumference of each second ball valve (512) rotates by friction with the inner circumference of the second connecting sphere (506). A second connecting hole (513) interconnected with the pipeline (505) is processed on each of the second ball valves (512). A second connecting rod (511) is provided on each of the second ball valves (512). A plurality of first motors (510) are provided at the bottom of the cabinet (15). The output shaft of each first motor (510) is fixedly connected to the second connecting rod (511).

6. The portable Solanaceae pollen transport device according to claim 4, characterized in that: The positioning mechanism (4) is as follows: a plurality of pull rods (404) are connected to the support plate (3) in a sliding manner in a vertical direction, a handle (401) is provided at the top of each pull rod (404), a top positioning plate (405) for positioning the top side of the liquid nitrogen tank (501) is provided at the bottom of each pull rod (404), a first spring (402) is provided on each pull rod (404), one end of each first spring (402) is fixedly connected to the top positioning plate (405), and the other end of each first spring (402) is fixedly connected to the bottom of the support plate (3), and a guide rod (403) is provided at the top of each top positioning plate (405) and is connected to the support plate (3) in a sliding manner in a vertical direction.

7. The portable Solanaceae pollen transport device according to claim 1, characterized in that: The clamping mechanism (8) comprises: a first rotating shaft (802) and a second rotating shaft (803) are mounted on the fixed housing (7) in a vertical direction for rotation; a third rotating wheel (801) is arranged on the top of the first rotating shaft (802); a first gear (804) is arranged on the bottom of the first rotating shaft (802); a second gear (805) meshing with the first gear (804) is arranged in the middle of the second rotating shaft (803); and a plurality of clamping components are arranged on the second rotating shaft (803); the clamping components comprise: a second connecting shaft (810) and a first connecting shaft (808) are mounted on the fixed housing (7) in a horizontal direction for rotation; and a third rotating wheel (801) is arranged on one end of the second connecting shaft (810). A clamping cam disc (809) is provided for clamping the cabinet door (1) and the cabinet body (15). The inclined surface of the clamping cam disc (809) contacts and clamps the inclined surface of the cabinet door (1). A fourth gear (811) is provided at the other end of the second connecting shaft (810). A third gear (807) meshing with the fourth gear (811) is provided at one end of the first connecting shaft (808). A second bevel gear (812) is provided at the other end of the first connecting shaft (808). A first bevel gear (806) meshing with the second bevel gear (812) is provided on the second rotating shaft (803). The clamping cam discs (809) in two adjacent clamping assemblies rotate in opposite directions.

8. The portable Solanaceae pollen transport device according to claim 1, characterized in that: One side panel of each inner shell (16) is processed with a plurality of first ventilation openings (17), and one side panel of the cabinet (15) is processed with a second ventilation opening (22) interconnected with the first ventilation opening (17). The side panel of the cabinet (15) is provided with a one-way valve mechanism (9) for blocking the second ventilation opening (22). The one-way valve mechanism (9) is as follows: the side panel of the cabinet (15) is connected to a plurality of sliding rods (903) in a horizontal sliding direction, and each sliding rod (903) is provided with a third spring (904), one end of each third spring (904) is fixedly connected to one end of the sliding rod (903), and the other end of each third spring (904) is fixedly connected to the inner wall of the side panel of the cabinet (15), and the plurality of sliding rods (903) are fixedly connected to the connecting plate (902), and the connecting plate (902) is provided with a sealing disk (901) for blocking the second ventilation opening (22).

9. A method for using a portable Solanaceae pollen transport device, characterized in that: Using the portable Solanaceae pollen transport device according to claim 2 comprises the following steps: S1. Pretreatment: Put pollen of Solanaceae male parent species into multiple pollen boxes (11) respectively, and precool them in a 4°C environment for 2 hours; S2. Loading: Push multiple carrier plates (12) into the inner shell (16) respectively, push the clamping grooves (19) on both sides of each carrier plate (12) horizontally along the clamping convex plate (18) inside the inner shell (16), and at the same time, the clamping grooves (19) on both sides of the carrier plate (12) are clamped with the clamping convex plate (18), the sealing cover (13) covers the upper part of the carrier plate (12), the sealing convex block (20) at the bottom of the sealing cover (13) seals the open end of the pollen box (11), close the cabinet door (1), and the clamping mechanism (8) The cabinet door (1) and the cabinet body (15) are clamped together, and each positioning mechanism (4) respectively positions the top of the cooling mechanism (5). When the cooling mechanism (5) is replaced, the positioning mechanism (4) is separated from the top of the cooling mechanism (5). Each cooling mechanism (5) cools each sealed space. The temperature sensor (14) in each sealed space is used to monitor the temperature in real time, so that the storage temperature in the inner shell (16) is 12° C. The one-way valve mechanism (9) on the cabinet body (15) is used to balance the internal and external air pressures of the cabinet body (15); S3. The cabinet (15) is transported as a whole to the Solanaceae female parent pollen base, the cabinet door (1) is opened, the driving mechanism (6) drives the sealing cover (13) to separate from the carrying plate (12), the carrying plate (12) in the inner shell (16) is pulled out, the Solanaceae male parent pollen in the pollen box (11) is taken out, and the Solanaceae male parent pollen is cross-pollinated with the Solanaceae female parent pollen.

10. The method for using the portable Solanaceae pollen transport device according to claim 9, characterized in that: The method for clamping the cabinet door (1) and the cabinet body (15) by the clamping mechanism (8) in S2 is as follows: manually rotating the third rotating wheel (801), the third rotating wheel (801) drives the first gear (804) on the first rotating shaft (802) to rotate, the first gear (804) and the second gear (805) are engaged and transmitted, the second gear (805) drives the second rotating shaft (803) to rotate, the second rotating shaft (803) drives the multiple fastening components to rotate until the cabinet door (1) and the cabinet body (15) are fastened, the fastening cam plates (809) in the two adjacent fastening components rotate in opposite directions, and the cabinet door (1) is clamped from different directions. The door (1) and the cabinet body (15) are fastened, wherein the method of fastening the assembly is as follows: the second rotating shaft (803) drives the first bevel gear (806) to rotate, the first bevel gear (806) and the second bevel gear (812) are meshed and transmitted, the second bevel gear (812) drives the third gear (807) of the first connecting shaft (808) to rotate, the third gear (807) and the fourth gear (811) are meshed and transmitted, the fourth gear (811) drives the clamping cam plate (809) to rotate through the second connecting shaft (810), and the clamping cam plate (809) rotates until it is fastened with the cabinet door (1) and the cabinet body (15); The method for cooling each sealed space by each cooling mechanism (5) in the S2 is as follows: manually rotating the first rotating wheel (503), the first rotating wheel (503) drives the first ball valve (509) to rotate through the first connecting rod (507), the first connecting hole (508) on the first ball valve (509) rotates to the outlet end of the liquid nitrogen tank (501) and the inlet end of the pipeline (505) to be connected with each other, the output shaft of the first motor (510) drives the second ball valve (512) to rotate through the second connecting rod (511), the second connecting hole (513) on the second ball valve (512) rotates to the pipeline (505) to be connected with each other, and the liquid nitrogen in the liquid nitrogen tank (501) enters the sealed space through the first connecting ball (502), the first connecting hole (508) of the first ball valve (509), the second connecting hole (513) of the second ball valve (512), and the pipeline (505) in sequence to cool down; The method for separating the positioning mechanism (4) and the top of the cooling mechanism (5) in S2 is as follows: manually pulling the handle (401), the handle (401) drives the pull rod (404) to move upward in the vertical direction on the support plate (3), the pull rod (404) drives the top positioning plate (405) to move in the vertical direction, the guide rod (403) on the top positioning plate (405) moves in the vertical direction on the support plate (3), and the top positioning plate (405) moves until it is separated from the liquid nitrogen tank (501); The one-way valve mechanism (9) on the cabinet (15) in S2 is used to balance the internal and external air pressure of the cabinet (15) in the following manner: under the action of the third spring (904), the slide bar (903) moves horizontally on the cabinet (15), and the gas in the inner shell (16) controls the sealing disk (901) to separate from the second vent (22) of the cabinet (15) or the sealing disk (901) covers the second vent (22) through the first vent (17) and the second vent (22), thereby balancing the internal and external air pressure of the cabinet (15); The method for driving the sealing cover (13) and the carrier plate (12) to be separated by the driving mechanism (6) in S3 is as follows: manually rotating the second rotating wheel (605), the second rotating wheel (605) drives the cams (606) at both ends of the third connecting rod (607) to rotate, each cam (606) moves to contact with the other end of the interference rod (604), and the inclined surface at one end of the interference rod (604) moves to contact with the interference wheel (603). Under the action of the second spring (602), the two interference wheels (603) drive the sealing cover (13) to move in the vertical direction on the two side plates of the inner shell (16), and the sealing cover (13) is separated from the carrier plate (12).