Microbial domestication of saline soils with assisted dispersed irrigation devices and methods

Abstract

The present application relates to the field of microorganism for saline-alkali soil treatment, especially to a device and method for saline-alkali soil microbial domestication, dispersion and assisted irrigation. The bottom segment (8) is a cylindrical structure with a bottom cone (10), and an outer through hole (9) is arranged on the wall of the cylindrical structure; the domestication center (7) can be placed in the bottom segment (8); the domestication center (7) is a cylindrical structure, which contains a plurality of outer infiltration holes, and contains a porous or support structure; a microbial substrate and microorganisms are arranged in the porous or support structure; the outlet pipe connecting port (6) and the inlet pipe connecting port (11) are connected with the inlet pipe (1) and the outlet pipe (2); and the device further comprises an outer cover (4) which can be sleeved above the bottom segment (8); the inlet pipe (1) and the outlet pipe (2) can provide and / or extract substances for the domestication center (7).

Description

Technical Field

[0001] This invention relates to the field of microbial use in the treatment of saline-alkali land, and more particularly to a device and method for the domestication and dispersal of microorganisms for irrigation in saline-alkali land. Background Technology

[0002] Microbial technology has demonstrated enormous application potential in the field of saline-alkali land management, injecting new vitality into the sustainable development of this sector. However, the implementation of this technology cannot yield significant results quickly in the short term. When applying microbial technology to saline-alkali land management, local conditions must be comprehensively considered, including climate conditions, soil characteristics, and the types of crops grown. Climate conditions, such as sunshine duration, temperature fluctuations, rainfall frequency, and wind conditions, significantly impact the survival and reproduction of microorganisms. For example, in arid regions with large diurnal temperature variations, microorganisms may require stronger drought and temperature tolerance. Soil characteristics, including soil texture (sandy, loam, clay), pH, salinity, and fertility, are also essential factors to consider when developing management plans. Different soil conditions are suitable for different types of microorganisms to survive and function. Simultaneously, the types of crops grown locally are also crucial; different crops have different soil environmental requirements, and the application of microbial technology must meet these requirements, creating a favorable growing environment for the crops. The significant differences in climate, soil, and crops across different regions undoubtedly increase the complexity and difficulty of using microbial technology to manage saline-alkali land, requiring in-depth research and analysis by professionals to develop scientific, reasonable, and feasible management solutions.

[0003] Currently, the application of microbial technology in saline-alkali land management is still in its developmental stage, and the overall application level needs further improvement. Strengthening the research and development and promotion of microbial technology is the mainstream direction for future saline-alkali land management. Through continuous research and development, we can gain a deeper understanding of the mechanisms of action of microorganisms in saline-alkali environments and develop more targeted and efficient microbial products. Widespread promotion will allow more saline-alkali lands to benefit from microbial technology, fully leveraging its role in improving soil quality and increasing crop yields.

[0004] The compound microbial inoculant used for saline-alkali land remediation contains a variety of microorganisms, such as Aspergillus oryzae, yeast, Streptomyces flavus, Lactobacillus acidophilus, Lactobacillus plantarum, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, and Bacillus polymyxa. These microorganisms coexist in the microbial inoculum solution and work synergistically to remediate saline-alkali land. After entering the soil, beneficial microorganisms can form a symbiotic relationship with some existing soil microorganisms. In this process, beneficial microorganisms can inhibit the growth of harmful bacteria. Exogenous beneficial bacteria can exert their effects by inhibiting the growth of harmful bacteria among native microorganisms (through the secretion of antibacterial active substances or nutrient competition) or by increasing the abundance of beneficial bacteria.

[0005] Meanwhile, these microorganisms produce a large number of metabolic products, playing a crucial role in the decomposition and transformation of organic matter. The decomposed organic matter provides crops with abundant nutrients and stimulating substances, promoting crop growth and development. Furthermore, it increases soil porosity and permeability, improving soil aeration and water permeability, thereby increasing plant survival rates.

[0006] Although microorganisms exhibit excellent performance in laboratory environments, they often die rapidly when scaled up and applied to harsh natural saline-alkali land environments. This is because natural saline-alkali land environments differ significantly from laboratory simulation environments. Laboratory environments are relatively stable and controllable, while natural saline-alkali land environments are complex and variable, including high soil salinity, harsh climatic conditions, and other unknown biotic and abiotic factors. Therefore, further acclimatization of microorganisms, allowing them to gradually adapt to the harsh natural environment, could potentially help the strains achieve long-term survival and enhance their resilience to environmental stress. This will be an important future development direction for microbial technology in saline-alkali land management, and through continuous exploration and practice, it is hoped that the survival rate and management effectiveness of microorganisms in saline-alkali land environments can be improved.

[0007] On June 6, 2025, a search was conducted in the China Patent Publication Database using "microbial strains and domestication and treatment and saline-alkali land" as the abstract keywords and the option to allow synonym expansion was selected. No relevant literature was found.

[0008] On June 6, 2025, an abstract search was conducted on CNKI (China National Knowledge Infrastructure) using the keywords "microbial strains and domestication and treatment of saline-alkali land". The results included: "Study on interspecific convergent evolution and intraspecific variation effects of highly efficient phosphate-solubilizing bacteria in salt-tolerant phosphate-solubilizing bacteria"; "Creation and application of widely adaptable and efficient microbial agents for rapid treatment and disposal of waste biomass"; "Research and integrated demonstration of key technologies for the production of fuel ethanol from whole sugar beets"; and "Screening, identification and characteristic study of highly efficient salt-tolerant bacteria". While these technologies systematically addressed issues related to microorganisms in saline-alkali land, they failed to fill the gap between pilot-scale testing and practical application, and did not provide suitable methods to further deepen the understanding of problems in the microbial domestication process.

[0009] On June 6, 2025, a search was conducted on the U.S. Patent and Trademark Office website for "Strain with domestication with control with saline-alkal", but no relevant literature was found; the search URL is https: / / ppubs.uspto.gov / pubwebapp / .

[0010] On June 6, 2025, a search was conducted on WIPO's website https: / / patentscope2.wipo.int / for the title "Strain and domestication and control and saline-alkali", but no relevant literature was found.

[0011] On June 6, 2025, a search was conducted on the website of the Japan Patent Office (https: / / www.j-platpat.inpit.go.jp / ) for the search term "Strain and domestication and control and saline-alkali", but no relevant literature was found.

[0012] It is completely different from the concept of this patent.

[0013] The shortcomings of these technologies are that they cannot fill the gap between pilot-scale testing and practical application, nor do they provide a suitable way to further deepen the problems in the microbial domestication process. Summary of the Invention

[0014] Purpose of the invention: To provide a more effective device and method for dispersing and irrigating microorganisms in saline-alkali land, the specific purpose of which is described in the detailed implementation section for several substantial technical effects.

[0015] To achieve the above objectives, the present invention adopts the following technical solution:

[0016] A saline-alkali land microbial domestication and dispersion-assisted planting irrigation device, characterized in that...

[0017] The ground-insertion irrigation device includes a bottom segment 8, which is a cylindrical structure with a bottom cone 10, and an external opening 9 is arranged on the wall of the cylindrical structure.

[0018] The domestication center 7 can be placed in the bottom segment 8;

[0019] The domestication center 7 is a cylindrical structure, which includes a threaded outer cover 13, on which an outlet pipe connection port 6 and an inlet pipe connection port 11 are arranged; the cylindrical structure includes multiple external seepage holes, and contains a porous or support structure; the porous or support structure contains a microbial substrate and microorganisms;

[0020] The outlet pipe connection port 6 and the inlet pipe connection port 11 are connected to the inlet pipe 1 and the outlet pipe 2, respectively.

[0021] It also includes an outer cover 4 that can be fitted over the bottom segment 8;

[0022] Inlet pipe 1 and outlet pipe 2 can provide and / or extract substances to the domestication center 7.

[0023] A further technical solution of the present invention is that the outer cover 4 includes an outer cover 12 for sealing, and the outer cover 12 includes two holes for the inlet pipe 1 and the outlet pipe 2 to pass through.

[0024] A further technical solution of the present invention is that an embedded extrusion portion 5 is arranged below the outer cover 4 for extruding into the upper end of the bottom segment 8.

[0025] A further technical solution of the present invention is that the outer wall of the extrusion portion 5 includes a rubber layer.

[0026] A further technical solution of the present invention is that multiple ground-inserted irrigation devices are connected to each other through inlet pipe 1 and outlet pipe 2, and inlet pipe 1 and outlet pipe 2 are connected to a water tank.

[0027] The method for dispersing and assisting in the irrigation of saline-alkali land microorganisms is characterized by,

[0028] Using any of the above-mentioned saline-alkali land microbial domestication and dispersion-assisted planting irrigation devices,

[0029] Includes Mode 1:

[0030] Microorganisms cultivated in the laboratory for the treatment of saline-alkali land;

[0031] After being expanded for cultivation, they were placed inside the domestication center;

[0032] A microbial domestication and dispersion auxiliary irrigation device for saline-alkali land is placed in the saline-alkali land.

[0033] Direct external water supply helps microorganisms adapt to the local saline-alkali soil;

[0034] Gradually reduce the water supply to allow the microorganisms to integrate with the surrounding soil, while periodically collecting semi-domesticated microorganisms for storage and further research;

[0035] Gradually apply laboratory microorganisms to saline-alkali land so that they can survive.

[0036] A further technical solution of the present invention includes an optional mode two:

[0037] The medium that absorbs salt is placed inside the acclimatization center;

[0038] Because the domestication center has an external opening that connects to the outside world, salt will gradually diffuse into the interior of the domestication center;

[0039] Periodically extract the medium back to the center for elution and purification, and replace it with a new medium;

[0040] To achieve the precipitation and transfer of salts in the soil environment.

[0041] A further technical solution of the present invention includes an optional mode three:

[0042] Multiple sets of saline-alkali land microbial domestication and dispersion auxiliary irrigation devices are placed in semi-underground locations.

[0043] Regular supplemental irrigation is used to deliver water to a depth of 10-50 cm underground.

[0044] The present invention, which adopts the above technical solution, has the following beneficial effects compared with the prior art: it is used to deeply integrate laboratory microorganisms with specific saline-alkali soil environments and further domesticate them. At the same time, it allows microorganisms to be directly in the soil at a certain depth, avoiding direct killing by sunlight and some adverse natural factors on the surface, increasing the survival rate of microorganisms, constructing a certain semi-domesticated environment, and also providing new equipment and devices for the environmental domestication of microorganisms. Attached Figure Description

[0045] To further illustrate the present invention, the following description is provided in conjunction with the accompanying drawings:

[0046] Figure 1 This is a schematic diagram of the invention.

[0047] Figure 2 A schematic diagram illustrating the usage state of the invention;

[0048] Figure 3 A schematic diagram showing another perspective on the use of the invention;

[0049] Figures 4-6 The diagram shows the implementation of the three modes of the invention;

[0050] Figure 7 and Figure 8 This is a partial structural diagram of the invention;

[0051] The components are: 1. Inlet pipe; 2. Outlet pipe; 3. Inner extension pipe; 4. Outer cover; 5. Embedded extrusion part; 6. Outlet pipe connection port; 7. Domestication center; 8. Bottom segment; 9. Outer port; 10. Bottom cone; 11. Inlet pipe connection port; 12. Outer cover; 13. Threaded outer cover. Detailed Implementation

[0052] The present invention will be further illustrated below with reference to the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. In the description of the present invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," "top," and "bottom," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. In addition, unless otherwise expressly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication of two components. For those skilled in the art, the specific meaning of the above terms in the present invention can be understood according to the specific circumstances.

[0053] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0054] This patent provides multiple parallel solutions; the different descriptions represent improved solutions or parallel solutions based on the basic solution. Each solution has its own unique characteristics. Furthermore, the technical features involved in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other. Fixing methods not described herein can be any type of fixing, such as threaded fixing, bolt fixing, or adhesive bonding.

[0055] Example 1: Referring to all the attached drawings; A device for dispersing and assisting in the cultivation of microorganisms in saline-alkali land for irrigation, characterized in that...

[0056] The ground-insertion irrigation device includes a bottom segment 8, which is a cylindrical structure with a bottom cone 10, and an external opening 9 is arranged on the wall of the cylindrical structure.

[0057] The domestication center 7 can be placed in the bottom segment 8;

[0058] The domestication center 7 is a cylindrical structure, which includes a threaded outer cover 13, on which an outlet pipe connection port 6 and an inlet pipe connection port 11 are arranged; the cylindrical structure includes multiple external seepage holes, and contains a porous or support structure; the porous or support structure contains a microbial substrate and microorganisms;

[0059] The outlet pipe connection port 6 and the inlet pipe connection port 11 are connected to the inlet pipe 1 and the outlet pipe 2, respectively.

[0060] It also includes an outer cover 4 that can be fitted over the bottom segment 8;

[0061] Inlet pipe 1 and outlet pipe 2 can provide and / or extract substances to the domestication center 7.

[0062] The substantive technical effects and implementation process of the technical solution presented herein, i.e., its basic functions, are as follows:

[0063] The method for dispersing and assisting in the irrigation of saline-alkali land microorganisms is characterized by,

[0064] Using any of the above-mentioned saline-alkali land microbial domestication and dispersion-assisted planting irrigation devices,

[0065] Includes Mode 1:

[0066] Microorganisms cultivated in the laboratory for the treatment of saline-alkali land;

[0067] After being expanded for cultivation, they were placed inside the domestication center;

[0068] A microbial domestication and dispersion auxiliary irrigation device for saline-alkali land is placed in the saline-alkali land.

[0069] Direct external water supply helps microorganisms adapt to the local saline-alkali soil;

[0070] Gradually reduce the water supply to allow the microorganisms to integrate with the surrounding soil, while periodically collecting semi-domesticated microorganisms for storage and further research;

[0071] Gradually apply laboratory microorganisms to saline-alkali land so that they can survive.

[0072] An acclimatization center is a complex structure used to house culture media, a basic framework, and microbial mucus. This complex primarily achieves mixing, long-term sustained release, and placement of microorganisms. Its structure can be a perforated honeycomb plate filled with mixed culture media, a basic framework, and microbial mucus; it can also be a shelf-like structure with multiple layers of culture media, a basic framework, and microbial mucus; or it can be a combination of porous materials and culture media, a basic framework, and microbial mucus. Microbial mucus refers to a mixture of microorganisms and culture media.

[0073] It also includes an optional mode two:

[0074] The medium that absorbs salt is placed inside the acclimatization center;

[0075] Because the domestication center has an external opening that connects to the outside world, salt will gradually diffuse into the interior of the domestication center;

[0076] Periodically extract the medium back to the center for elution and purification, and replace it with a new medium;

[0077] To achieve the precipitation and transfer of salts in the soil environment.

[0078] It also includes an optional mode three:

[0079] Multiple sets of saline-alkali land microbial domestication and dispersion auxiliary irrigation devices are placed in semi-underground locations.

[0080] Regular supplemental irrigation is used to deliver water to a depth of 10-50 cm underground.

[0081] A further technical solution of the present invention is that the outer cover 4 includes an outer cover 12 for sealing, and the outer cover 12 includes two holes for the inlet pipe 1 and the outlet pipe 2 to pass through.

[0082] Example 2: As a further improvement, parallel solution, or optional independent solution, an embedded extrusion portion 5 is arranged below the outer cover 4 for extruding into the upper end of the bottom segment 8. The substantial technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: This embodiment provides a specific docking method and structure, and similar implementation structures are all within the protection scope of this patent.

[0083] Example 3: As a further improvement, parallel, or optional independent solution, the outer wall of the extrusion part 5 includes a rubber layer. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, are as follows: extrusion is more convenient, and the sealing effect is better.

[0084] Example 4: As a further improvement, parallel, or optional independent solution, multiple ground-inserted irrigation devices are connected to each other via inlet pipe 1 and outlet pipe 2, which are connected to a water tank. The substantive technical effect and implementation process of this technical solution, i.e., its basic function, is as follows: to periodically inject water into the microbial community, ensuring that microorganisms can survive in a dry underground environment.

[0085] Increasing the survival probability of microorganisms provides the basic conditions for the spread of microbial strains to the surrounding soil.

[0086] You can also irrigate directly into the soil, allowing microorganisms to permeate the surrounding area.

[0087] Water is injected and retained, and the amount of water supplied is gradually reduced to force microorganisms to gradually adapt to the surrounding environment.

[0088] Innovatively, each of the above effects exists independently, yet a single structure can be used to combine the results.

[0089] It should be noted that the multiple modules in this patent are an integration of existing technology modules and do not involve any new modules. Even if some modules use programs, those programs are undoubtedly known programs.

[0090] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims.

Claims

1. A device for dispersing and assisting in the cultivation of microorganisms in saline-alkali land for irrigation, characterized in that, The ground-insertion irrigation device includes a bottom segment (8), which is a cylindrical structure with a bottom cone (10) and an external opening (9) arranged on the wall of the cylindrical structure. The acclimatization center (7) can be placed in the bottom segment (8); The acclimatization center (7) is a cylindrical structure, which includes a threaded outer cover (13), on which an outlet pipe connection port (6) and an inlet pipe connection port (11) are arranged; the cylindrical structure includes multiple external leakage holes, and the cylindrical structure contains a porous or support structure; the porous or support structure contains microbial substrate and microorganisms; The outlet connection port (6) and the inlet connection port (11) are connected to the inlet pipe (1) and the outlet pipe (2); It also includes an outer cover (4) that can be fitted over the bottom segment (8); The inlet pipe (1) and outlet pipe (2) can provide and / or extract substances to the domestication center (7).

2. The saline-alkali land microbial domestication and dispersion-assisted planting irrigation device as described in claim 1, characterized in that, The outer cover (4) includes an outer cover (12) for sealing, and the outer cover (12) includes two holes for the inlet pipe (1) and the outlet pipe (2) to pass through.

3. The saline-alkali land microbial domestication and dispersion-assisted planting irrigation device as described in claim 1, characterized in that, The outer cover (4) has a pressing part (5) arranged below it for pressing into the upper end of the bottom segment (8).

4. The saline-alkali land microbial domestication and dispersion-assisted planting irrigation device as described in claim 3, characterized in that, A rubber layer is provided on the outer wall of the extrusion part (5).

5. The saline-alkali land microbial domestication and dispersion-assisted planting irrigation device as described in claim 1, characterized in that, Multiple ground-inserted irrigation devices are connected to each other via inlet pipe (1) and outlet pipe (2), which are connected to a water tank.

6. A method for dispersing and assisting in the irrigation of saline-alkali land microorganisms, characterized in that, Using the saline-alkali land microbial domestication and dispersion-assisted planting irrigation device according to any one of claims 1-5, Includes Mode 1: Microorganisms cultivated in the laboratory for the treatment of saline-alkali land; After being expanded for cultivation, they were placed inside the domestication center; Place the saline-alkali land microbial domestication and dispersion auxiliary irrigation device in the saline-alkali land; Direct external water supply helps microorganisms adapt to the local saline-alkali soil; Gradually reduce the water supply to allow the microorganisms to integrate with the surrounding soil, while periodically collecting semi-domesticated microorganisms for storage and further research; To enable laboratory microorganisms to survive in saline-alkali soil.

7. The method for dispersing and assisting in the irrigation of saline-alkali land microorganisms as described in claim 6, characterized in that, It also includes Mode 2: The medium that absorbs salt is placed inside the acclimatization center; Because the domestication center has an external opening that connects to the outside world, salt will gradually diffuse into the interior of the domestication center; Periodically extract the medium for elution and purification, and replace it with a new medium; To achieve the precipitation and transfer of salts in the soil environment.

8. The method for dispersing and assisting in the irrigation of saline-alkali land microorganisms as described in claim 6, characterized in that, It also includes Mode 3: Multiple groups of saline-alkali land microbial domestication and dispersion auxiliary irrigation devices are placed underground; Regular supplemental irrigation is used to deliver water to a depth of 10-50 cm underground.

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