Anti-allergy repair composition containing lindera aggregata and andrographis paniculata, and preparation method and application thereof

Abstract

The invention provides an anti-allergy repairing composition containing lindera aggregata and andrographis paniculata as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The technical problem to be solved is to provide the cosmetic containing the lindera aggregata and the andrographis paniculata, which has repairing and anti-allergy effects and is simple and safe in components. The key points of the technical scheme are as follows: 1) a preparation method of a lindera aggregata extract and an andrographis paniculata extract; 2) a lindera aggregata extract and an andrographis paniculata extract in a specific ratio; and 3) application of the composition containing the lindera aggregata extract and the andrographis paniculata extract in anti-allergy repair cosmetics. The components are extracted from Chinese herbal medicines, and the components are simple, so that the traditional Chinese medicine composition is safer to use. Meanwhile, a good anti-allergy effect can be achieved by inhibiting TRPV1 receptor activation, HaCaT cells can be promoted to express FLG and LOR, and a remarkable repairing effect is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics. Specifically, the present application provides an anti-allergic repair composition containing magnolia bark and andrographis, a preparation method and application thereof. BACKGROUND

[0002] Technical content for understanding the present application: With the improvement of living standards and consumption concept, people pay more and more attention to the safety and efficacy performance of cosmetics, and the consciousness of pursuing natural, green, health and safety is increasing. This change is due to two realistic needs: on the one hand, with the increasing social pressure from work, life and other aspects and the increasingly serious environmental pollution, leading to sensitive problems such as skin redness, itching and so on; on the other hand, in the face of more and more types of cosmetics, consumers pay more and more attention to cosmetic products with safe ingredients. Therefore, people's demand for cosmetics with natural anti-allergic, anti-inflammatory, soothing and repairing efficacy is also increasing. Cosmetics containing natural Chinese herbal medicine active ingredients are safe to use, widely applicable, and meet the concepts of environmental protection, green, natural, safety and pursuit of efficacy.

[0003] Compared with traditional cosmetics, cosmetics prepared with plant extracts as active ingredients have many advantages, such as: overcoming the shortcomings of traditional cosmetics relying on chemical synthesis, making the safety performance of the product higher; natural components are more easily absorbed by the skin, making the effect of the product more significant; more prominent function, etc.

[0004] The common soothing and repairing anti-allergic cosmetics on the market mostly achieve the repair effect by enhancing the moisturizing effect. However, simply relying on moisturizing can only temporarily alleviate the irritation of the skin, and cannot solve the core problem of fragile and sensitive skin from the root.

[0005] Just like many products on the market add hyaluronic acid, ceramide and other active substances, which are good moisturizing ingredients, by supplementing water and lipids, promoting the repair process of keratinocyte cells, thereby restoring its barrier function, and reducing the sensitization reaction of irritants (such as sodium dodecyl sulfate).

[0006] As the literature: Clinical observation on the auxiliary treatment effect of skin care products containing spilanthes and licorice extract on sensitive skin, published on July 15, 2009, discloses that the skin care products containing spilanthes and licorice extract significantly increased the water content of the stratum corneum of dry and neutral sensitive skin (from 71.53% to 74.49%) within 30 days, and improved the problem of imbalance of sebum secretion. The study also found that the lactic acid test value decreased (from 4.39 to 2.58), indicating the recovery of skin barrier function. This result directly proves that the moisturizing ingredients indirectly promote barrier repair by increasing the water content of the skin.

[0007] Research findings: Lindera contains rich furan sesquiterpenes, lactones, volatile oils, flavonoids, isoquinoline alkaloids, etc., and has antibacterial and anti-inflammatory analgesic effects.

[0008] The patent discloses a lindera extract gel with skin regeneration and repair effect and a preparation method thereof, and specifies that lindera extract is the only active ingredient in the drug. As a natural and safe drug for promoting skin regeneration and repair, the drug can promote wound healing and skin regeneration by reducing the release of inflammatory factors. The lindera extract gel specifies a specific crude drug concentration, and claims that the active ingredient is safe and mild, and the local administration avoids the potential risk of adverse reactions caused by systemic administration, but the anti-allergic effect is not studied.

[0009] Andrographis contains lactones and flavonoids. Among them, andrographolide significantly restores skin barrier function and reduces inflammatory response by inhibiting nuclear transcription factor (NF-kB) and regulating the expression of skin barrier-related proteins such as FLG, IVL, and LOR, thereby improving skin health.

[0010] The patent discloses a natural plant extract composition with soothing, repairing and anti-allergic effects, which contains radix bupleuri extract, radix bletillae extract, radix sophorae flavescentis extract, citrus fruit extract, radix stephaniae tetrandrae extract and andrographis extract. The natural plant extract composition containing andrographis extract can cooperate with each other and has a synergistic effect, which can jointly control skin inflammation, improve skin allergy, itching and stinging, and make the skin in a more stable state. However, the invention uses multiple plant extracts, which may have a potential sensitization risk.

[0011] Research findings show that there are few reports on using lindera and andrographis as cosmetics for repair and anti-allergy. The patent discloses a cosmetic with moisturizing function prepared by using lindera extract and andrographis extract as a component for repairing circadian rhythm, but the anti-allergic repair effect is not studied.

[0012] Therefore, there is an urgent need for a composition with repair and anti-allergic effects and a preparation method thereof, which is simple and safe in composition. SUMMARY

[0013] One of the purposes of the present application is to provide: The application discloses an anti-allergic repair composition containing magnolia bark and andrographis, a preparation method and application thereof, and related technologies, and aims at solving the technical problems of complex composition and poor anti-allergic soothing effect in the prior art or a combination thereof.

[0014] In a first aspect, the application provides an anti-allergic repair composition containing magnolia bark and andrographis, wherein the anti-allergic repair composition comprises magnolia bark extract and andrographis extract.

[0015] The anti-allergic repair composition comprises the following technical features: a mass ratio of the magnolia bark extract and the andrographis extract, a preparation method of the magnolia bark extract, a preparation method of the andrographis extract, and the anti-allergic repair composition.

[0016] The mass ratio of the magnolia bark extract and the andrographis extract is 1:9-5:5.

[0017] The mass ratio of the magnolia bark extract and the andrographis extract is preferably 1:9, 1:5, 1:6, 1:7, 1:8, 2:5, 2:6, 2:7, 2:8, 2:9, 3:5, 3:6, 3:7, 3:8, 3:9, 4:5, 4:6, 4:7, 4:8, 4:9, 5:5, 5:6, 5:7, 5:8 or 5:9. The mass ratio of the magnolia bark extract and the andrographis extract is more preferably 1:9, 1:7, 1:8, 3:6, 3:7, 3:8, 5:5, 5:6 or 5:7. The mass ratio of the magnolia bark extract and the andrographis extract is further preferably 1:9, 3:7 or 5:5. The mass ratio of the magnolia bark extract and the andrographis extract is still further preferably 3:7.

[0018] The preparation method of the magnolia bark extract is as follows: The magnolia bark is extracted with water, filtered, and the filtrate is left to stand, filtered again, and the impurities in the filtrate are removed, concentrated and filtered again to obtain the magnolia bark extract.

[0019] As a preferred embodiment, the water is deionized water. Preferably, the water is added in a mass of 10-20 times the mass of the magnolia bark. More preferably, the water is added in a mass of 10 times, 12 times, 14 times, 15 times, 16 times, 18 times or 20 times the mass of the magnolia bark. Further preferably, the water is added in a mass of 10 times, 12 times, 15 times, 16 times or 20 times the mass of the magnolia bark. Still further preferably, the water is added in a mass of 10 times, 15 times or 20 times the mass of the magnolia bark. Still further preferably, the water is added in a mass of 15 times the mass of the magnolia bark.

[0020] As a preferred embodiment, the temperature of the extraction is 90-100°C; Preferably, the temperature of the extraction is 90°C, 91°C, 92°C, 93°C, 94°C, 95°C, 96°C, 97°C, 98°C, 99°C or 100°C; More preferably, the temperature of the extraction is 90°C, 91°C, 92°C, 93°C, 94°C, 95°C or 100°C; Further preferably, the temperature of the extraction is 90°C, 93°C, 95°C or 100°C; Still further preferably, the temperature of the extraction is 90°C, 95°C or 100°C; Yet further preferably, the temperature of the extraction is 95°C.

[0021] As a preferred embodiment, the time of the extraction temperature is 1-3h; Preferably, the time of the extraction temperature is 1h, 1.5h, 2h, 2.5h or 3h; More preferably, the time of the extraction temperature is 1h, 1.5h, 2h or 3h; Further preferably, the time of the extraction temperature is 1h, 2h or 3h; Still further preferably, the time of the extraction temperature is 2h or 3h; Yet further preferably, the time of the extraction temperature is 2h.

[0022] As a preferred embodiment, the temperature of the standing is 0°C-4°C; Preferably, the temperature of the standing is 0°C, 0.5°C, 1°C, 1.2°C, 1.5°C, 1.8°C, 2°C, 2.3°C, 2.5°C, 2.7°C, 3°C, 3.2°C, 3.5°C, 3.6°C, 3.8°C or 4°C; More preferably, the temperature of the standing is 0°C, 1°C, 1.8°C, 2°C, 2.7°C, 3°C, 3.4°C, 3.8°C or 4°C; Further preferably, the temperature of the standing is 0°C, 2°C, 3°C, 3.8°C or 4°C; Still further preferably, the temperature of the standing is 2°C or 4°C; Yet further preferably, the temperature of the standing is 2°C.

[0023] As a preferred embodiment, the time of the standing is >3 days; Preferably, the time of the standing is 3 days, 3.5 days, 4 days, 4.5 days, 5 days, 5.5 days, 6 days, 6.5 days or 7 days; More preferably, the standing time is 3 days, 4 days, 4.5 days, 5 days, 6 days, 6.5 days or 7 days. Further preferably, the standing time is 3 days, 4 days, 6.5 days or 7 days. Still further preferably, the standing time is 3 days or 4 days. Still further preferably, the standing time is 3 days.

[0024] The impurity removal operation is removal of macromolecular substances through a 10KD molecular cut-off membrane, and removal of small molecular substances and inorganic salts through a 500D molecular cut-off membrane.

[0025] The concentration quality is the same as that of the Magnoliae officinalis extract.

[0026] As a most preferred embodiment, the Magnoliae officinalis extract preparation method is: S1, 100g Magnoliae officinalis is added to 1500g deionized water, and extracted at 95℃ for 2h, and the residue is removed; S2, standing at 2℃ for 4 days, and filtering to be clear; S3, removal of macromolecular substances through a 10KD molecular cut-off membrane, and removal of small molecular substances and inorganic salts through a 500D molecular cut-off membrane; S4, concentration to 100g, filtering to be clear, and obtaining the Magnoliae officinalis extract for standby use.

[0027] The Andrographis extract preparation method is: Andrographis is extracted with ethanol, filtered, and the filtrate is reserved for decolorization with activated carbon. After concentration, the pH is adjusted, and the precipitate is collected after standing. After water washing and filtering, 1,3-propanediol is added for stirring, and cooling to obtain the product.

[0028] As a preferred embodiment, the ethanol addition quality is 10-15 times the quality of the Andrographis. Preferably, the ethanol addition quality is 10 times, 11 times, 12 times, 14 times or 15 times the quality of the Andrographis. More preferably, the ethanol addition quality is 10 times, 11 times, 12 times or 15 times the quality of the Andrographis. Further preferably, the ethanol addition quality is 10 times, 12 times or 15 times the quality of the Andrographis. Still further preferably, the ethanol addition quality is 12 times the quality of the Andrographis.

[0029] As a preferred embodiment, the ethanol pH is 10-11. Preferably, the ethanol pH is 10, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9 or 11. More preferably, the ethanol pH is 10, 10.1, 10.2, 10.5, 10.8, 10.9 or 11; Further preferably, the ethanol pH is 10, 10.2, 10.8 or 11; Still further preferably, the ethanol pH is 10 or 11; Yet further preferably, the ethanol pH is 10.

[0030] As a preferred embodiment, the ethanol degree is 30-70°; Preferably, the ethanol degree is 30°, 35°, 40°, 45°, 50°, 55°, 60°, 65° or 70°; More preferably, the ethanol degree is 30°, 45°, 50°, 65° or 70°; Further preferably, the ethanol degree is 30°, 50° or 70°; Still further preferably, the ethanol degree is 50°.

[0031] As a preferred embodiment, the extraction is reflux extraction; Preferably, the reflux extraction temperature is 60-90°C; More preferably, the reflux extraction temperature is 60°C, 65°C, 70°C, 75°C, 80°C, 85°C or 90°C; Further preferably, the reflux extraction temperature is 60°C, 70°C, 75°C, 80°C, 85°C or 90°C; Still further preferably, the reflux extraction temperature is 60°C, 75°C or 90°C; Yet further preferably, the reflux extraction temperature is 75°C.

[0032] As a preferred embodiment, the reflux extraction time is 1-2h; Preferably, the reflux extraction time is 1h, 1.25h, 1.5h, 1.75h or 2h; More preferably, the reflux extraction time is 1h or 2h; Further preferably, the reflux extraction time is 1h.

[0033] As a preferred embodiment, the activated carbon added is 1-5% of the mass of Andrographis; Preferably, the activated carbon added is 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5% of the mass of Andrographis; More preferably, the amount of activated carbon added is 1%, 2%, 3%, 4% or 5% by weight of the Andrographis; Further preferably, the amount of activated carbon added is 1%, 3% or 5% by weight of the Andrographis; Still further preferably, the amount of activated carbon added is 3% by weight of the Andrographis.

[0034] As a preferred embodiment, the decolourisation of activated carbon is performed under stirring conditions; Preferably, the temperature of the stirring is 30-70°C; More preferably, the temperature of the stirring is 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C or 70°C; Further preferably, the temperature of the stirring is 30°C, 45°C, 50°C, 65°C or 70°C; Still further preferably, the temperature of the stirring is 30°C, 50°C or 70°C; Yet further preferably, the temperature of the stirring is 50°C.

[0035] As a preferred embodiment, the time of stirring is 10-30 minutes; Preferably, the time of stirring is 10 minutes, 15 minutes, 20 minutes, 25 minutes or 30 minutes; More preferably, the time of stirring is 10 minutes, 15 minutes, 20 minutes or 30 minutes; Further preferably, the time of stirring is 10 minutes, 20 minutes or 30 minutes; Still further preferably, the time of stirring is 20 minutes.

[0036] As a preferred embodiment, the mass after concentration after removal of alcohol is 1-3 times the mass of the Andrographis; Preferably, the mass after concentration after removal of alcohol is 1 times, 1.5 times, 2 times, 2.5 times or 3 times the mass of the Andrographis; More preferably, the mass after concentration after removal of alcohol is 1 times, 2 times or 3 times the mass of the Andrographis; Still further preferably, the mass after concentration after removal of alcohol is 2 times the mass of the Andrographis.

[0037] As a preferred embodiment, the temperature after concentration is 25°C.

[0038] As a preferred embodiment, the pH after concentration is adjusted to a range of 5-7; Preferably, the pH after concentration is adjusted to 5, 5.2, 5.5, 5.8, 6, 6.2, 6.5, 6.7 or 7; More preferably, the pH is adjusted to 5, 5.5, 5.8, 6, 6.5 or 7 after concentration; Further preferably, the pH is adjusted to 5, 6 or 7 after concentration; Still further preferably, the pH is adjusted to 6 after concentration; As a preferred embodiment, the time for standing and precipitating is ≥ 1 h; Preferably, the time for standing and precipitating is 1 h, 1.5 h, 2 h or 2.5 h; More preferably, the time for standing and precipitating is 1 h or 2 h; Further preferably, the time for standing and precipitating is 2 h.

[0039] As a preferred embodiment, the water added for washing is deionized water; Preferably, the mass of water added is 1-2 times the mass of Andrographis; More preferably, the mass of water added is 1 times, 1.2 times, 1.5 times, 1.8 times or 2 times the mass of Andrographis; Further preferably, the mass of water added is 1 times, 1.5 times or 2 times the mass of Andrographis; Still further preferably, the mass of water added is 1 times or 2 times the mass of Andrographis; Still further preferably, the mass of water added is 1 times the mass of Andrographis; The specific operation of washing and filtering is repeated washing until the conductivity of the washing liquid is lower than 100 μs / ml, and the water in the precipitate is sufficiently filtered out.

[0040] The mass of 1,3-propanediol added is the same as the mass of Andrographis.

[0041] As a preferred embodiment, the stirring is heating stirring; Preferably, the temperature of stirring is ≥ 90℃; More preferably, the temperature of stirring is 90℃, 92℃, 93℃, 94℃, 95℃, 98℃ or 100℃; Further preferably, the temperature of stirring is 90℃, 93℃, 95℃, 96℃ or 100℃; Still further preferably, the temperature of stirring is 90℃ or 95℃; Still further preferably, the temperature of stirring is 95℃.

[0042] As a preferred embodiment, the time for stirring is ≥ 1 h; Preferably, the time for stirring is 1 h, 1.5 h, 2 h, 2.5 h or 3 h; More preferably, the time of stirring is 1 h, 1.5 h or 2 h; Further preferably, the time of stirring is 1 h or 1.5 h; Still further preferably, the time of stirring is 1.5 h.

[0043] As a preferred embodiment, the cooling temperature range is 50-60°C; Preferably, the cooling temperature is 50°C, 51°C, 52°C, 53°C, 54°C, 55°C, 56°C, 57°C, 58°C, 59°C or 60°C; More preferably, the cooling temperature is 50°C, 51°C, 52°C, 55°C, 56°C, 58°C or 60°C; Further preferably, the cooling temperature is 50°C, 52°C, 55°C, 58°C or 60°C; Still further preferably, the cooling temperature is 50°C, 55°C or 60°C; Still further preferably, the cooling temperature is 55°C.

[0044] As a most preferred embodiment, the preparation method of the Andrographis extract is as follows: S1, 100 g of Andrographis is added to 1200 g of 50° ethanol with pH of 10, and extracted at 75°C for 1 h, and the residue is removed; S2, 3 g of activated carbon is added, and stirred at 50°C for 20 min; S3, the carbon is removed by filtration to be clear, and concentrated to 200 g; S4, the temperature is lowered to 25°C, the pH is adjusted to 6, and left to stand for 2 h, and the precipitate is collected; S5, 100 g of deionized water is added to the precipitate, and repeatedly washed until the conductivity of the washing liquid is lower than 100 μs / ml, and the water in the precipitate is sufficiently filtered out; S6, 100 g of 1,3-propanediol is added, and stirred at 95°C for 1.5 h, and the temperature is lowered to 55°C, and the Andrographis extract is obtained, and stored for use.

[0045] As a preferred embodiment, the composition for anti-allergic repair further comprises the following components: lecithin, phytosterol, Tween-80 and 1,3-propanediol.

[0046] As a preferred embodiment, the composition for anti-allergic repair comprises the following components by weight percentage: Lindera extract 1-5%; Andrographis extract 5-9%; Lecithin 1-10%; Phytosterol 0.1-5%; Tween-80 0.1-5%; 1,3-propanediol 5-50%; and water q.s. 100%.

[0047] As a preferred embodiment, the lecithin is 1-10% by weight percent; Preferably, the lecithin is 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% by weight percent; More preferably, the lecithin is 2%, 3%, 4%, 5%, 6%, 8%, 9%, or 10% by weight percent; Further preferably, the lecithin is 1%, 4%, 5%, 8%, 9%, or 10% by weight percent; Still further preferably, the lecithin is 3%, 5%, or 9% by weight percent; Still further preferably, the lecithin is 5% by weight percent.

[0048] Preferably, the phytosterol is one or more of a soy phytosterol, a phytosterol ester, a glycosylated phytosterol, and a PEG phytosterol.

[0049] As a preferred embodiment, the phytosterol is 0.1-5% by weight percent; Preferably, the phytosterol is 0.1%, 0.5%, 1%, 1.5%, 3%, or 5% by weight percent; More preferably, the phytosterol is 0.5%, 1%, 1.5%, 3%, or 5% by weight percent; Further preferably, the phytosterol is 0.5%, 1.5%, 3%, or 5% by weight percent; Still further preferably, the phytosterol is 0.5%, 3%, or 5% by weight percent; Still further preferably, the phytosterol is 3% by weight percent.

[0050] As a preferred embodiment, the Tween-80 is 0.1-5% by weight percent; Preferably, the Tween-80 is 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, or 5% by weight percent; More preferably, the Tween-80 is 1%, 1.5%, 2%, 2.5%, 3%, or 5% by weight percent; Further preferably, the Tween-80 is 1%, 1.5%, 3%, or 5% by weight percent; Still further preferably, the Tween-80 is 1.5%, 3%, or 5% by weight percent; Still further preferably, the Tween-80 is 3% by weight percent.

[0051] As a preferred embodiment, the 1,3-propanediol percentage is 5-50%; Preferably, the 1,3-propanediol percentage is 5%, 6%, 7%, 8%, 10%, 19%, 20%, 21%, 22%, 27%, 28%, 35%, 38%, 39%, 40%, 45%, or 50%; More preferably, the 1,3-propanediol percentage is 5%, 6%, 20%, 21%, 27%, 28%, 39%, 40%, or 50%; Further preferably, the 1,3-propanediol percentage is 5%, 6%, 20%, 40%, or 50%; Still further preferably, the 1,3-propanediol percentage is 6%, 20%, or 40%; Still further preferably, the 1,3-propanediol percentage is 20%.

[0052] As a most preferred embodiment, the anti-sensitivity repair composition comprises the following components by weight percentage: Lindera extract 3%; Andrographis extract 7%; Lecithin 5%; Phytosterol 3%; Tween-80 3%; 1,3-propanediol 20%; and Water to 100%.

[0053] In a second aspect, the present application provides a method for preparing an anti-sensitivity repair composition.

[0054] The method for preparing the anti-sensitivity repair composition comprises the following steps: (1) preparing an oil phase: mixing the Andrographis extract with lecithin, phytosterol, Tween, and 1,3-propanediol, and heating and stirring to obtain the oil phase; (2) preparing an aqueous phase: mixing the Lindera extract with water, and heating and stirring to obtain the aqueous phase; (3) mixing the oil phase and the aqueous phase to obtain a crude emulsion, and homogenizing the crude emulsion to obtain the anti-sensitivity repair composition.

[0055] As a preferred embodiment, the temperature for stirring in step (1) is 70-80°C; Preferably, the temperature for stirring when preparing the oil phase is 70°C, 71°C, 72°C, 73°C, 74°C, 75°C, 76°C, 77°C, 78°C, 79°C, or 80°C; More preferably, the temperature for stirring when preparing the oil phase is 70°C, 73°C, 74°C, 75°C, 76°C, 77°C, or 80°C; Further preferably, the temperature of stirring during preparation of the oil phase is 70°C, 74°C, 75°C, 76°C or 80°C. Still further preferably, the temperature of stirring during preparation of the oil phase is 70°C, 75°C or 80°C. Yet further preferably, the temperature of stirring during preparation of the oil phase is 75°C.

[0056] As a preferred embodiment, the temperature of stirring during step (2) is 70-80°C. Preferably, the temperature of stirring during preparation of the water phase is 70°C, 71°C, 72°C, 73°C, 74°C, 75°C, 76°C, 77°C, 78°C, 79°C or 80°C. More preferably, the temperature of stirring during preparation of the water phase is 70°C, 73°C, 74°C, 75°C, 76°C, 77°C or 80°C. Further preferably, the temperature of stirring during preparation of the water phase is 70°C, 74°C, 75°C, 76°C or 80°C. Still further preferably, the temperature of stirring during preparation of the water phase is 70°C, 75°C or 80°C. Yet further preferably, the temperature of stirring during preparation of the water phase is 75°C.

[0057] As a preferred embodiment, the specific operation step of step (3) is: circulating and homogenizing the coarse emulsion through a high-pressure homogenizer for 3 times, to obtain the composition.

[0058] In a third aspect, the present application provides: use of the above-mentioned anti-sensitivity repair composition in the preparation of an anti-sensitivity repair cosmetic.

[0059] The cosmetic is a toner, emulsion, essence, cream, mask, foundation liquid, foundation cream, air cushion, sunscreen, body milk, hair conditioner or hair mask.

[0060] In a fourth aspect, the present application provides: an anti-sensitivity repair cosmetic, which comprises the above-mentioned anti-sensitivity repair composition and a cosmetically acceptable auxiliary.

[0061] The auxiliary is selected from one or more of the following: humectants, skin conditioning agents, thickening agents, emulsifiers, film-forming agents, ultraviolet absorbers, antioxidants, preservatives, pH adjusting agents, solvents, penetration enhancers and lubricants.

[0062] Explanation of terms: Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this subject matter belongs. Unless otherwise specified, all patents, applications, publications, and other documents referred to herein are hereby incorporated by reference in their entirety. In the event of a conflict between the definitions in this section and those in the remainder of the specification, the definitions in this section prevail. Terms of art have the meanings as commonly understood by those of ordinary skill in the art.

[0063] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the subject matter claimed. In this application, the use of the singular includes the plural, unless specifically stated otherwise. It must be noted that as used herein and in the appended claims, the singular forms "a", "an" and "the" include plural reference unless the context clearly dictates otherwise. In addition, the use of "or" as a conjunction is intended to mean "and / or," unless specifically stated otherwise. Furthermore, the use of the term "including" as well as other forms such as "contain," "comprise," "comprises," "has," "have," "including," and "includes" should be considered as specifically referring to the objects or elements "in the process of," or "comprising" but not limiting of the same.

[0064] Unless specifically indicated otherwise, the use of each of the types of commercially available products used herein is in accordance with standard techniques. For example, the use of kits can be in accordance with the manufacturer's instructions, or in accordance with well-known methods in the art or the instructions of the present application. In general, the techniques and methods described above can be performed in accordance with conventional methods well-known in the art, as described in a number of general and more specific texts referenced and discussed in the present specification.

[0065] As used herein, the term "Linderae Radix" refers to: Linderae Radix is the dried root of Lindera aggregata (Sims) Kosterm. It is acrid and warm in nature. It belongs to the lung, spleen, kidney and bladder meridians. Its functions are to move qi and relieve pain, and to warm the kidney and dispel cold. It is used to treat chest and abdominal distending pain, dyspnea, and kidney yang deficiency and bladder cold. The main chemical components include sesquiterpenes and their lactones, such as linderol ether lactone, pseudo-new linderol ether lactone, linderol, linderene, etc.; alkaloids, such as mukuroziol, boldine, norisoboldine, etc.; fatty acids, such as decanoic acid, dodecanoic acid, etc.; volatile oils, such as borneol, borneol acetate, etc.

[0066] As used herein, the term "Andrographis Herba" refers to: Andrographis Herba is the dried aerial part of Andrographis paniculata (Burm. f.) Nees (Chinese Pharmacopoeia 2010 edition). The main active components in Andrographis Herba are andrographolide, dehydroandrographolide, neoandrographolide, deoxyandrographolide, etc., which are traditional anti-inflammatory ingredients.

[0067] As used herein, the term "combination index" refers to: A method for evaluating the combined effect of drugs, proposed by Chou and Talalay in 1984. This method is based on the dose-effect curve of drugs, by comparing the actual effect and the theoretical effect of combined use, to judge whether the combined use has synergistic effect, additive effect or antagonistic effect.

[0068] The terms "FLG" and "LOR" used herein refer to: The skin barrier is the first line of defense against the invasion of external adverse factors, and its function mainly depends on the formation of the stratum corneum. The stratum corneum differentiation proteins include filaggrin (FLG), involucrin (IVL), loricrin (LOR), keratinocyte transglutaminase, keratin 1 (K1) and keratin 10 (K10). FLG and LOR are key factors for maintaining barrier function. LOR is equivalent to the armor of keratinocytes, making keratinocytes more solid, and FLG supports the internal structure of keratinocytes, and after degradation, it is the main source of natural moisturizing factor (NMF).

[0069] Based on the further solution or simultaneous solution of the technical problems of the present application, in the technical scheme provided in the first aspect of the present application, the preferred scheme comprises: The first preferred scheme: a preparation method of an anti-allergic repair composition containing Lindera and Andrographis. On the basis of solving the technical problems of complex components and poor anti-allergic effect, the technical scheme further solves the shallow repair performed by only moisturizing.

[0070] In the present application, examples 1-5 support the protection scope of claims 1-2, 13-16.

[0071] For claims 1-2, 13-16: The technical feature "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5", which is obtained by generalizing the technical features "containing Lindera extract and Andrographis extract, and the mass ratio is 1:9, 3:7 and 9:9" in the foregoing explanation and / or examples 1-5 of the present application. Therefore, the person skilled in the art can reasonably infer that the technical features "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5", the lower concept of "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5", the basic equivalent technical means of "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5", and the technical means of "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5" based on the existing technical level within the conventional technical means and common knowledge should all belong to the protection scope of claim 1, for example, under the condition that other technical features remain unchanged, replacing "containing Lindera extract and Andrographis extract, and the mass ratio is 1-9:5-5" with Lindera extract and Andrographis extract, and the mass ratio is 2:7, 3:6 and 4:8, etc., still belongs to the protection scope of claim 1 of the present application.

[0072] The examples 1-5 in the present application at least support the protection scope of claims 3-5.

[0073] For claims 3-5: The technical feature "preparation method of specific magnolia extract" is summarized by the common feature "water added is deionized water, mass ratio of deionized water to magnolia, and heating extraction" from the aforementioned explanations and / or the corresponding technical features "water added is deionized water, mass ratio of deionized water to magnolia is 10-20:1, and extraction at 90-100℃ for 1-3h" in examples 1-5. Therefore, the skilled person in the art can reasonably infer that the basic equivalent technical means of the technical feature "preparation method of specific magnolia extract", and the technical means that can replace the preparation method of specific magnolia extract based on the existing technical level within the conventional technical means and common knowledge, should all belong to the protection scope of claims 3-5, for example, under the condition that other technical features remain unchanged, replacing the preparation method of specific magnolia extract with the mass ratio of deionized water to magnolia being 12:1, 16:1 and 19:1, extraction at 90℃ for 2h, extraction at 92℃ for 2h, and extraction at 100℃ for 2h, etc., still belongs to the protection scope of claims 3-5 of the present application.

[0074] The examples 1-5 in the present application at least support the protection scope of claims 6-12.

[0075] For claims 6-12: The technical feature "preparation method of specific andrographis extract" is summarized by the common feature "mass ratio of ethanol and andrographis, pH and degree of ethanol, temperature and time of reflux extraction, mass of activated carbon, temperature and time of stirring of 10-30 minutes, cooling temperature, adjusted pH of 5-7, precipitation time" from the aforementioned explanation and / or the corresponding technical features "mass ratio of ethanol and andrographis of 10-15:1, pH of ethanol of 10-11 and degree of 30-70°, temperature of reflux extraction of 60-90°C and time of 1-2h, mass of activated carbon of 1-5% of andrographis, temperature of stirring of 30-70°C and time of 10-30 minutes, cooling temperature of 25°C, adjusted pH of 5-7, precipitation time of greater than or equal to 1h" in Examples 1-5. Therefore, the skilled person in the art can reasonably infer that the technical features "preparation method of specific andrographis extract", "lower concept of preparation method of specific andrographis extract", "basic equivalent technical means of preparation method of specific andrographis extract", and "technical means of preparation method of specific andrographis extract that can be replaced within conventional technical means and common knowledge based on the existing technical level" should all belong to the protection scope of claims 6-12, for example, the mass ratio of ethanol and andrographis is 13:1, the degree of ethanol is 60°, etc., which still belongs to the protection scope of claims 6-12 of the present application.

[0076] Examples 1-5 in the present application support at least the protection scope of claims 13-14.

[0077] For claims 13-14: The technical feature "composition of anti-allergic repair composition" is summarized by the common feature "content of lindera extract, andrographis extract, lecithin, phytosterol, Tween-80, 1,3-propanediol and water" from the aforementioned explanation and / or the corresponding technical features "lindera extract 1-5%, andrographis extract 5-9%, lecithin 1-10%, phytosterol 0.1-5%, Tween-80 0.1-5%, 1,3-propanediol 5-50% and water to 100%" in Examples 1-5. Therefore, the skilled person in the art can reasonably infer that the technical features "composition of anti-allergic repair composition", "lower concept of composition of anti-allergic repair composition", "basic equivalent technical means of composition of anti-allergic repair composition", and "technical means of composition of anti-allergic repair composition that can be replaced within conventional technical means and common knowledge based on the existing technical level" should all belong to the protection scope of claims 13-14, for example, lindera extract 2%, andrographis extract 8%, lecithin 6%, phytosterol 4%, Tween-80 0.3%, 1,3-propanediol 42% and water to 100%, etc., which still belongs to the protection scope of claims 13-14 of the present application.

[0078] The embodiments 1-5 in the present application support the protection scope of claim 16 at least.

[0079] For claim 16: The technical feature "preparation method of the anti-allergic repair composition" is summarized by the common feature "the temperature for stirring during preparation of the oil phase and the water phase is 70-80 DEG C, and the crude emulsion obtained by mixing the oil phase and the water phase is homogenized" from the aforementioned explanations and / or the corresponding technical features in the embodiments 1-5 such as "the temperature for stirring during preparation of the oil phase is 70-80 DEG C, the temperature for stirring during preparation of the water phase is 70-80 DEG C, and the crude emulsion obtained by mixing the oil phase and the water phase is homogenized". Therefore, the technical features anti-allergic repair composition preparation method, the subordinate concept of the anti-allergic repair composition preparation method, the basic equivalent technical means of the anti-allergic repair composition preparation method, and the technical means of the anti-allergic repair composition preparation method which can be replaced within the conventional technical means and common knowledge based on the existing technical level, can be determined by the person skilled in the art by reasonable inference, and should belong to the protection scope of claims 13-14, for example, the temperature for stirring during preparation of the oil phase is 72 DEG C, the temperature for stirring during preparation of the water phase is 76 DEG C, and the crude emulsion obtained by mixing the oil phase and the water phase is homogenized, etc., which still belongs to the protection scope of claim 16 of the present application.

[0080] Compared with the prior art, the present application has the following advantages: The present application provides a preparation method of an anti-allergic repair composition containing lindera and andrographis, which is used to produce a cosmetic with simple and safe ingredients, and has the effects of anti-allergy and repair. The present application has significant anti-allergic effect by inhibiting the activation of TRPV1 receptor, and has significant repair effect by promoting the expression of FLG and LOR in HaCaT cells.

[0081] In addition, based on the case of the present application: Based on the comparison of the embodiments 1-5 and the comparative examples 1-2, the anti-allergic repair composition of the present application uses the technical means of using lindera extract and andrographis extract in a specific ratio, which can significantly inhibit the activation of TRPV1 receptor, has significant anti-allergic effect, and can promote the expression of FLG and LOR in HaCaT cells, has significant repair effect. The combined technical effect is more superior than the sum of the effects of each technical means.

[0082] Based on the comparison of Examples 1-5 and Comparative Examples 3-6, the anti-allergic repair composition of the application prepared by using the technical means of specific preparation method of the extract of Lindera aggregata and the extract of Andrographis paniculata not only can better inhibit the activation of TRPV1 receptor, has better anti-allergic effect; but also can better promote the expression of FLG and LOR of HaCaT cells, has better repair effect.

[0083] Based on the comparison of Examples 1-5 and Comparative Example 7, the extract of Andrographis paniculata and the combination of the extract of Lindera aggregata and the extract of Andrographis paniculata prepared by using the technical means of specific preparation method of the application have significant anti-allergic and repair effects, and Comparative Example 7 shows no synergistic inhibition effect; compared with Examples 1-5, the effect of the expression of FLG and LOR of HaCaT cells in Comparative Example 7 is lower than that of Examples 1-5, and the repair effect is also obviously weakened. DETAILED DESCRIPTION

[0084] The following non-limiting examples can make those skilled in the art more fully understand the application, but do not limit the application in any way. The following content is only an exemplary description of the scope of the application claimed by the application, and those skilled in the art can make various changes and modifications to the application according to the disclosed content, and it should also belong to the scope of the application claimed by the application.

[0085] The application is further described below in the form of specific examples. The various instruments, devices, equipment, reagents, products, etc. used in the examples of the application are obtained through conventional commercial channels unless otherwise specified.

[0086] Example 1 An anti-allergic repair composition containing Lindera and Andrographis paniculata and a preparation method thereof The following components are in weight percentage: Lindera extract 1%, Andrographis paniculata extract 9%, lecithin 3%, phytosterol ester 0.5%, Tween-80 1.5%, 1,3-propanediol 6% and water 79%; The preparation method is: (1) Preparation method of Lindera extract: (1.1) 100 g of Lindera is added to 1000 g of deionized water, and extracted at 90°C for 1 h, and the residue is removed; (1.2) Place at 4°C for 3 days, filter to clarify; (1.3) Remove macromolecular substances through a 10KD molecular cut-off membrane, and then remove small molecular substances and inorganic salts through a 500D molecular cut-off membrane; (1.4) Concentrate to 100 g, filter to clarify, and obtain the Lindera extract, which is ready for use.

[0087] (2) Preparation method of Andrographis paniculata extract: (2.1) 100g Andrographis added to 1000g 30° ethanol with pH 10, reflux extraction at 60°C for 1h, remove residue; (2.2) Add 1g activated carbon, stir at 50°C for 10min; (2.3) Decarburization filtration to clear, concentrate to 100g; (2.4) Cool to 25°C, adjust pH to 5, stand for 1h, collect precipitate; (2.5) Add 100g deionized water to the precipitate, repeatedly wash until the wash liquid conductivity is less than 100μs / ml, filter the water from the precipitate; (2.6) Add 100g 1,3-propanediol, stir at 90°C for 1h, cool to 50°C, obtain Andrographis extract, keep warm for standby.

[0088] (3) Prepare oil phase: take the Andrographis extract prepared in step (2), add lecithin, phytosterol ester, Tween-80, 1,3-propanediol, heat to 75°C and stir to mix; (4) Prepare water phase: take the Lindera extract prepared in step (1) and mix with water, heat to 75°C and stir to mix; (5) Mix the two phases after stirring to obtain a crude emulsion, pass the crude emulsion through a high-pressure homogenizer for 3 cycles to obtain water-soluble liposomes, thus obtaining the composition.

[0089] Example 2: An anti-allergic repair composition containing Lindera and Andrographis and a preparation method thereof The components are as follows in terms of weight percentage: Lindera extract 3%, Andrographis extract 7%, lecithin 5%, phytosterol ester 3%, Tween-80 3%, 1,3-propanediol 20%, and water 59%; The preparation method is as follows: (1) Preparation method of Lindera extract: (1.1) 100g Lindera added to 1500g deionized water, extract at 95°C for 2h, remove residue; (1.2) Place at 2°C for 4 days, filter to clear; (1.3) Remove macromolecular substances through a 10KD molecular cut-off membrane, then remove small molecular substances and inorganic salts through a 500D molecular cut-off membrane; (1.4) Concentrate to 100g, filter to clear, obtain Lindera extract, keep for standby.

[0090] (2) Preparation method of Andrographis extract: (2.1) 100g Andrographis added to 1200g 50° ethanol with pH 10, reflux extraction at 75°C for 1h, remove residue; (2.2) Add 3 g activated carbon, stir at 50 °C for 20 min; (2.3) Decarburize to filter to clear, concentrate to 200 g; (2.4) Cool to 25 °C, adjust pH to 6, stand for 2 h, collect the precipitate; (2.5) Add 100 g deionized water to the precipitate, repeatedly wash until the wash liquid conductivity is less than 100 μs / ml, filter the water from the precipitate; (2.6) Add 100 g 1,3-propanediol, stir at 95 °C for 1.5 h, cool to 55 °C, obtain andsroside extract, and keep warm for standby.

[0091] (3) Prepare oil phase: take the androside extract prepared in step (2), add lecithin, phytosterol ester, Tween-80, 1,3-propanediol, heat to 75 °C, and stir to mix; (4) Prepare water phase: take the lindera extract prepared in step (1) and water, heat to 75 °C, and stir to mix; (5) Mix the two phases after stirring to obtain a crude emulsion, and pass the crude emulsion through a high-pressure homogenizer for 3 cycles to obtain water-soluble liposomes, thus obtaining the composition.

[0092] Example 3: An anti-allergic repair composition containing lindera andandroside and a preparation method thereof The components are as follows in terms of weight percentage: Lindera extract 5%, androside extract 5%, lecithin 9%, phytosterol ester 5%, Tween-80 5%, 1,3-propanediol 40%, and water 31%; The preparation method is as follows: (1) Preparation method of lindera extract: (1.1) Add 100 g lindera to 2000 g deionized water, extract at 100 °C for 3 h, and remove the residue; (1.2) Place at 0 °C for 3 days, filter to clear; (1.3) Remove macromolecular substances through a 10 KD molecular cut-off membrane, and then remove small molecular substances and inorganic salts through a 500 D molecular cut-off membrane; (1.4) Concentrate to 100 g, filter to clear, and obtain lindera extract for standby.

[0093] (2) Preparation method of androside extract: (2.1) Add 100 g androside to 1500 g 70 ° ethanol with pH of 11, extract at 90 °C for 2 h, and remove the residue; (2.2) Add 3 g activated carbon, stir at 50 °C for 20 min; (2.3) Decarburize to filter to clear, concentrate to 200 g; (2.4) cooling to 25 °C, adjusting pH to 7, standing for 1 h, collecting the precipitate; (2.5) adding 200 g of deionized water to the precipitate, repeatedly washing until the conductivity of the washing liquid is less than 100 μs / ml, and filtering the precipitate to remove water; (2.6) adding 100 g of 1,3-propanediol, stirring at 90 °C for 1 h, cooling to 60 °C, obtaining andresgraphis extract, and keeping warm for standby use.

[0094] (3) preparing an oil phase: taking the andresgraphis extract prepared in step (2), adding lecithin, phytosterol ester, Tween-80, 1,3-propanediol, heating to 75 °C, and stirring to mix; (4) preparing an aqueous phase: taking the lindera extract prepared in step (1) and mixing with water, heating to 75 °C, and stirring to mix; (5) mixing the two phases after stirring to obtain a crude emulsion, and subjecting the crude emulsion to a high-pressure homogenizer for 3 cycles of homogenization to obtain water-soluble liposomes, thus obtaining the composition.

[0095] Example 4: an anti-sensitivity repair composition containing lindera and andresgraphis and a preparation method thereof the following components by weight percentage: lindera extract 3%, andresgraphis extract 7%, lecithin 10%, phytosterol ester 3%, Tween-80 3%, 1,3-propanediol 5%, and water 69%; the preparation method is as follows: (1) a preparation method of lindera extract: (1.1) adding 100 g of lindera to 1500 g of deionized water, extracting at 95 °C for 2 h, and removing the residue; (1.2) standing at 2 °C for 4 days, and filtering to be clear; (1.3) removing macromolecular substances through a 10 KD molecular cut-off membrane, and removing small molecular substances and inorganic salts through a 500 D molecular cut-off membrane; (1.4) concentrating to 100 g, filtering to be clear, obtaining lindera extract, and keeping for standby use.

[0096] (2) a preparation method of andresgraphis extract: (2.1) adding 100 g of andresgraphis to 1200 g of 50 ° ethanol with pH of 10, refluxing and extracting at 75 °C for 2 h, and removing the residue; (2.2) adding 3 g of activated carbon, stirring at 30 °C for 20 min; (2.3) filtering to be clear after decarbonization, and concentrating to 200 g; (2.4) cooling to 25 °C, adjusting pH to 6, standing for 2 h, and collecting the precipitate; (2.5) The precipitate is added with 100 g of deionized water, and repeatedly washed until the conductivity of the washing solution is lower than 100 μs / ml, and the water in the precipitate is filtered out; (2.6) 100 g of 1,3-propanediol is added, stirred at 95 °C for 1.5 h, cooled to 55 °C, and the andrographis extract is obtained and stored for use.

[0097] (3) Preparation of oil phase: the andrographis extract prepared in step (2) is taken, lecithin, phytosterol ester, Tween-80, and 1,3-propanediol are added, heated to 75 °C, and stirred to mix; (4) Preparation of water phase: the lindera extract prepared in step (1) is taken, mixed with water, heated to 75 °C, and stirred to mix; (5) The obtained coarse emulsion is mixed by stirring, and the coarse emulsion is subjected to circulation homogenization for 3 times by a high-pressure homogenizer to obtain the water-soluble liposome, and the composition is obtained.

[0098] Example 5: An anti-allergic repair composition containing lindera and andrographis and a preparation method thereof The components are as follows in percentage by weight: Lindera extract 3%, andrographis extract 7%, lecithin 1%, phytosterol ester 3%, Tween-80 3%, 1,3-propanediol 50%, and water 33%; The preparation method is as follows: (1) Preparation method of lindera extract: (1.1) 100 g of lindera is added with 1500 g of deionized water, extracted at 95 °C for 2 h, and the residue is removed; (1.2) Stored at 2 °C for 4 days, and filtered to be clear; (1.3) Large molecular substances are removed through a 10 KD molecular cut-off membrane, and small molecular substances and inorganic salts are removed through a 500 D molecular cut-off membrane; (1.4) Concentrated to 100 g, filtered to be clear, and the lindera extract is obtained and stored for use.

[0099] (2) Preparation method of andrographis extract: (2.1) 100 g of andrographis is added with 1200 g of 50 ° ethanol with pH of 10, extracted at 75 °C for 2 h by reflux, and the residue is removed; (2.2) 3 g of activated carbon is added, and stirred at 70 °C for 20 minutes; (2.3) Filtered to be clear by decarbonization, and concentrated to 200 g; (2.4) Cooled to 25 °C, adjusted to pH of 6, and stored for 2 h, and the precipitate is collected; (2.5) The precipitate is added with 100 g of deionized water, and repeatedly washed until the conductivity of the washing solution is lower than 100 μs / ml, and the water in the precipitate is filtered out; (2.6) Add 100 g 1,3-propanediol, stir at 95°C for 1.5 h, cool to 55°C, obtain Andrographis extract, and keep warm for standby.

[0100] (3) Preparation of oil phase: take the Andrographis extract prepared in step (2), add lecithin, phytosterol ester, Tween-80, 1,3-propanediol, heat to 75°C and stir to mix; (4) Preparation of water phase: take the Lindera extract prepared in step (1) and mix with water, heat to 75°C and stir to mix; (5) Mix the two phases to obtain a crude emulsion, and then pass the crude emulsion through a high-pressure homogenizer for circulation homogenization for 3 times to obtain water-soluble liposomes, thereby obtaining the composition.

[0101] Comparative Example 1 The difference from Example 2 is that: The Lindera extract is 10% by weight, and no Andrographis extract is added; The preparation method is as follows: (1) Preparation method of Lindera extract: (1.1) 100 g of Lindera is added to 1000 g of deionized water, and extracted at 90°C for 1 h, and the residue is removed; (1.2) Place at 4°C for 3 days, and filter to clarify; The content of the remaining components is the same as in Example 2.

[0102] Comparative Example 2 The difference from Example 2 is that: The Andrographis extract is 10% by weight, and no Lindera extract is added; (2) Preparation method of Andrographis extract: (2.1) 100 g of Andrographis is added to 1000 g of 30° ethanol with pH of 10, and extracted at 60°C for 1 h, and the residue is removed; (2.2) Add 1 g of activated carbon, and stir at 50°C for 10 minutes; (2.3) Filter to clarify after decarburization, and concentrate to 100 g; (2.4) Cool to 25°C, adjust the pH to 5, and stand for 1 h, and collect the precipitate; (2.6) Add 100 g of 1,3-propanediol, stir at 90°C for 1 h, cool to 50°C, obtain Andrographis extract, and keep warm for standby.

[0103] The content of the remaining components is the same as in Example 2.

[0104] Comparative Example 3 The difference from Example 2 is that: The preparation method is as follows: (1) Preparation method of extract of L. latifolia: (1.1) 100 g of L. latifolia was added to 2500 g of deionized water, and extracted at 90°C for 2 h, and the residue was removed; (1.2) It was placed at 4°C for 4 days, and filtered to be clear; (2) Preparation method of extract of A. paniculata: (2.1) 100 g of A. paniculata was added to 2000 g of 50° ethanol with pH of 10; The component content and the remaining steps were the same as in Example 2.

[0105] Comparative Example 4 The difference from Example 2 is that: The preparation method is: (1) Preparation method of extract of L. latifolia: (1.1) 100 g of L. latifolia was added to 1500 g of deionized water, and extracted at 80°C for 4 h, and the residue was removed; (1.2) It was placed at 4°C for 4 days, and filtered to be clear; (2) Preparation method of extract of A. paniculata: (2.1) 100 g of A. paniculata was added to 1200 g of 50° ethanol, and extracted at 50°C for 3 h, and the residue was removed; (2.2) 1 g of activated carbon was added; The component content and the remaining steps were the same as in Example 2.

[0106] Comparative Example 5 The difference from Example 2 is that: The preparation method is: (1) Preparation method of extract of L. latifolia: (1.3) Without removing macromolecular substances, small molecular substances and inorganic salts through 10KD and 500D molecular cut-off membranes; (2) The difference between the preparation method of extract of A. paniculata and the basic Example 2-2 is that: (2.2) 10 g of activated carbon was added, and stirred at 80°C for 60 min; The component content and the remaining steps were the same as in Example 2.

[0107] Comparative Example 6 The difference from Example 2 is that: The preparation method is: (2) Preparation method of extract of A. paniculata: (2.2) 10 g of activated carbon was added; (2.4) The temperature was lowered to 25°C, the pH was adjusted to 4, and the precipitate was collected after standing for 0.5 h; The component content and the remaining steps were the same as in Example 2.

[0108] Comparative Example 7 The difference from Example 2 is that: The content of the magnolia extract is 9% and the content of the andrographis extract is 1% by weight percentage. The preparation method is as follows: (2) Preparation method of andrographis extract: (2.6) 100 g of 1,3-propanediol is added, stirred at 95°C for 1.5 h, cooled to 25°C, and the andrographis extract is obtained and stored for use.

[0109] The content of the remaining components and the steps are the same as those of Example 2.

[0110] Effect Example 1: Cytotoxicity test of the composition 1. Experimental method The compositions prepared in Examples 1-5 and Comparative Examples 1-7 are diluted to 500 ppm, 100 ppm, 20 ppm and 4 ppm respectively in DMEM medium under sterile conditions to obtain the test samples.

[0111] A suspension of human keratinocytes (HaCaT) in the logarithmic phase and growing well is inoculated in a 96-well plate, with 8×10 3 cells per well, and incubated for 24 h. Different concentrations of the test sample are added, and incubated for another 24 h. Then, the cells are washed twice with PBS, 100 μL of CCK-8 working solution (CCK-8 reagent: DMEM medium = 1:10) is added to each well, and incubated at 37°C in a 5% CO2 cell incubator for 2 h. The absorbance is measured at 450 nm by a microplate reader.

[0112] Cell relative survival rate (%) = (OD value of sample group / OD value of control group) × 100% 2. Experimental results Table 1

[0113] As can be seen from the results in Table 1, the samples have no cytotoxicity in the test range, and 500 ppm, 100 ppm and 20 ppm are selected for the following tests.

[0114] Effect Example 2: Anti-allergic effect of the composition 1. Experimental method The sample preparation method is the same as that of Effect Example 1.

[0115] A suspension of HaCaT cells in the logarithmic phase and growing well is inoculated in a black 96-well plate, with 8×10 3One, incubate 24 h, then incubate with 4 μM Fluo-4 AM at 37℃, 5% CO2 cell incubator for 30 min. After washing with D-hank's three times, add different concentrations of samples to be tested and 5 μM capsaicin, incubate at 37℃, 5% CO2 cell incubator for 30 min, then measure the fluorescence intensity at EX / EM=485 / 525 nm by fluorescence microplate reader to obtain F0; then add 10 μM capsaicin (CAP) for stimulation, measure the fluorescence intensity at EX / EM=485 / 525 nm by fluorescence microplate reader to obtain F1. The experimental groups are as follows: Table 2

[0116] Note: " / " indicates that no sample to be tested is added.

[0117] Inhibition rate (%) = ((negative control group F1-negative control group F0) - (each group F1-each group F0)) / (negative control group F1-negative control group F0) x 100%.

[0118] The inhibition rate calculated is analyzed by CompuSyn software to obtain the combination index CI (Combination Index). The combination index is a method for evaluating the combination of drugs, which was proposed by Chou and Talalay in 1984. Based on the dose-effect curve of drugs, by comparing the actual effect and the theoretical effect of combined drugs, it is judged whether the combined drugs have synergistic effect, additive effect or antagonistic effect. CI value less than 0.9 indicates synergistic effect, the smaller the value, the stronger the synergistic effect; CI value between 0.9-1.1 indicates additive effect; CI value greater than 1.1 indicates antagonistic effect, the larger the value, the stronger the antagonistic effect.

[0119] 2. Experimental results According to the results in Table 3 below, examples 1-5 and comparative examples 1-7 all have a significant inhibitory effect on the activation of TRPV1 receptors at a concentration of 500 ppm. Compared with comparative examples 1-7, examples 1-5 have a stronger inhibitory effect on TRPV1 receptors. Examples 1-5 prepared by combining magnolia extract and andrographis extract, and comparative examples 3-6 all have a synergistic inhibitory effect on the activation of TRPV1 receptors. Compared with comparative examples 3-6, examples 1-5 have a stronger synergistic effect, and the CI value is between 0.3-0.5, while comparative example 7 has no synergistic effect.

[0120] Table 3

[0121] Note: " / " indicates that no sample to be tested is added.

[0122] Effect Example 3 Repairing effect of the composition 1. Experimental method The sample preparation was the same as that of Effect Example 1.

[0123] A suspension of HaCaT cells in logarithmic phase and in good growth was taken and inoculated in a 6-well plate containing 10% fetal bovine serum, 1% double-antibody DMEM medium, with 2x10 5 After 24h of incubation, different concentrations of the sample to be tested were added, and after 24h of further incubation, total RNA was extracted, cDNA amplification was performed using a kit, and the expression of FLG and LOR was detected in a real-time fluorescent quantitative PCR detection system.

[0124] 2. Experimental results The effect of the sample on the expression of FLG by HaCaT cells is shown in Table 4 below: Table 4

[0125] According to the experimental results in Table 4, Examples 1-4 all have the effect of promoting the expression of FLG by HaCaT cells at medium and high concentrations, and Comparative Examples 1-7 also have the effect of promoting the expression of FLG by HaCaT cells at high concentrations, but the effect is lower than that of Examples 1-4.

[0126] The effect of the sample on the expression of LOR by HaCaT cells is shown in Table 5 below: Table 5

[0127] According to the experimental results in Table 5, Examples 1-5 and Comparative Examples 1-7 all have the effect of promoting the expression of LOR by HaCaT cells at high concentrations, and the effect of Examples 1-5 is better than that of Comparative Examples 1-7.

[0128] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. An anti-allergic repair composition containing magnolia and andrographis, characterized in that, The composition comprises magnolia extract and andrographis extract; the mass ratio of the magnolia extract and the andrographis extract is 1:9-5:5; preferably 3:

7.

2. The anti-sensitivity repair composition according to claim 1, wherein The preparation method of the magnolia extract comprises the following steps: 1) filtering magnolia after water extraction; 2) taking the filtrate to stand, and filtering again after standing; 3) removing impurities through 10KD molecular cut-off membrane and 500D molecular cut-off membrane respectively; 4) concentrating and filtering to be clear to obtain the magnolia extract.

3. The anti-sensitivity repair composition according to claim 2, wherein The water in step 1) is deionized water; the mass ratio of the deionized water to magnolia is 10-20:1; the extraction temperature is 90-100℃, and the time is 1-3h.

4. The anti-sensitivity repair composition of claim 1, wherein The preparation method of the andrographis extract comprises the following steps: S1, adding ethanol to andrographis, removing residue after reflux extraction; S2, adding activated carbon and stirring; S3, filtering to be clear after decarbonization, and concentrating to remove alcohol; S4, cooling, adjusting pH, and precipitating to collect the precipitate; S5, taking the precipitate to wash, washing until the conductivity of the washing liquid is lower than 100us / ml, and drying; S6, adding 1,3-propanediol, heating and stirring, and obtaining the andrographis extract after cooling.

5. The anti-sensitivity repair composition according to claim 4, wherein The mass ratio of the ethanol to andrographis in step S1 is 10-15:1; the pH of the ethanol is 10-11, and the degree is 30-70°; the reflux extraction temperature is 60-90℃, and the time is 1-2h; the mass of the activated carbon in step S2 is 1-5% of the mass of andrographis; the stirring temperature is 30-70℃, and the stirring time is 10-30 minutes; the cooling temperature in step S4 is 25℃; the adjusted pH is 5-7, and the precipitation time is greater than or equal to 1h.

6. The anti-sensitivity repair composition of claim 1, wherein The anti-allergy repair composition further comprises the following components: lecithin, phytosterol, Tween-80 and 1,3-propanediol.

7. The anti-sensitivity repair composition according to claim 6, wherein The anti-allergy repair composition comprises the following components by weight percentage: Magnolia extract 1-5%; Andrographis extract 5-9%; Lecithin 1-10%; Phytosterol 0.1-5%; Tween-80 0.1-5%; 1,3-propanediol 5-50%; and Water to 100%.

8. Process for the preparation of an anti-sensitivity repair composition according to any one of claims 1 to 7, characterized in that, The preparation method comprises the following steps: (1) preparing an oil phase: mixing andrographis extract with lecithin, phytosterol, Tween and 1,3-propanediol, and heating and stirring to obtain the oil phase; (2) preparing an aqueous phase: mixing magnolia extract with water, and heating and stirring to obtain the aqueous phase; (3) mixing the oil phase and the aqueous phase to obtain a crude emulsion, and homogenizing the crude emulsion to obtain the anti-allergy repair composition.

9. Use of an anti-sensitivity repair composition according to any one of claims 1 to 7 for the preparation of an anti-sensitivity care cosmetic product, characterized in that: The cosmetic is toner, emulsion, essence, cream, mask, foundation liquid, foundation cream, air cushion, sunscreen, body milk, hair conditioner or hair mask.

10. An anti-sensitivity repair cosmetic product, characterized in that, The anti-allergy repair composition comprises the following components by weight percentage: Magnolia extract 1-5%; Andrographis extract 5-9%; Lecithin 1-10%; Phytosterol 0.1-5%; Tween-80 0.1-5%; 1,3-propanediol 5-50%; and Water to 100%. The preparation method comprises the following steps: (1) preparing an oil phase: mixing andrographis extract with lecithin, phytosterol, Tween and 1,3-propanediol, and heating and stirring to obtain the oil phase; (2) preparing an aqueous phase: mixing magnolia extract with water, and heating and stirring to obtain the aqueous phase; (3) mixing the oil phase and the aqueous phase to obtain a crude emulsion, and homogenizing the crude emulsion to obtain the anti-allergy repair composition. The cosmetic is toner, emulsion, essence, cream, mask, foundation liquid, foundation cream, air cushion, sunscreen, body milk, hair conditioner or hair mask. The anti-allergy repair composition comprises the following components by weight percentage: Magnolia extract 1-5%; Andrographis extract 5-9%; Lecithin 1-10%; Phytosterol 0.1-5%; Tween-80 0.1-5%; 1,3-propanediol 5-50%; and Water to 100%. The preparation method comprises the following steps: (1) preparing an oil phase: mixing andrographis extract with lecithin, phytosterol, Tween and 1,3-propanediol, and heating and stirring to obtain the oil phase; (2) preparing an aqueous phase: mixing magnolia extract with water, and heating and stirring to obtain the aqueous phase; (3) mixing the oil phase and the aqueous phase to obtain a crude emulsion, and homogenizing the crude emulsion to obtain the anti-allergy repair composition. The cosmetic is toner, emulsion, essence, cream, mask, foundation liquid, foundation cream, air cushion, sunscreen, body milk, hair conditioner or hair mask. The anti-allergy repair composition comprises the following components by weight percentage: Magnolia extract 1-5%; Andrographis extract 5-9%; Lecithin 1-10%; Phytosterol 0.1-5%; Tween-80 0.1-5%; 1,3-propanediol 5-50%; and Water to 100%.

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