A method for screening rhododendron anthora annual type varieties

Abstract

The application relates to a method for screening rhododendron annum varieties, which utilizes the cutting branch refrigeration technology to catalyze rhododendron to bloom, constructs an analytic hierarchy process according to refrigeration quality indexes such as the refrigeration freeze injury rate, the time required from the refrigeration warehouse to blooming, the branch blooming rate and blooming quality indexes such as the inflorescence opening degree and the inflorescence size, compares the blooming quality of the tested varieties with the pot flower of rhododendron as a contrast, the varieties with significant differences in the comprehensive scores and the pot flower are determined as unsuitable for blooming, and the varieties without significant differences are determined as suitable for blooming, that is, the rhododendron annum varieties. The application provides a method for screening rhododendron annum varieties, which is easy to implement and less harmful to the pot flower, can not only reduce the screening difficulty of the rhododendron annum varieties, but also effectively promote the large-scale production of self-bred rhododendron annum new varieties.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, specifically to a method for screening alpine rhododendron varieties suitable for the Lunar New Year. Background Technology

[0002] New Year's flowers refer to flowers that are concentrated on the market from around the Spring Festival to the Lantern Festival. They are mainly used for festive decoration and to add to the festive atmosphere. The name comes from the sales period "New Year's Day," which is from the end of the year to the Spring Festival. Some documents also refer to them as "New Year's sales flowers." In the past decade, alpine rhododendrons have become an important high-end New Year's flower and have been highly favored in the New Year's flower market in my country and Southeast Asian countries.

[0003] Currently, domestic research institutions have cultivated more than 10 new varieties of alpine rhododendron through germplasm innovation, effectively solving the problem of limited germplasm resources in the localization of alpine rhododendrons (Jie Weijia, Li Shifeng, Peng Luchun, et al. 12 new varieties of alpine rhododendron authorized [J]. China Flowers & Horticulture, 2023, (04): 44-45). However, the natural flowering period of alpine rhododendrons is concentrated in March to May, and in high-altitude areas it can be delayed to July to August. If you want it to bloom during the Spring Festival, you must first select varieties with an early flowering period, a sufficient number of well-developed flower buds and a good ornamental plant shape, and then carry out a 2 to 3 month low-temperature dormancy treatment to catalyze the flowering of alpine rhododendrons. However, the characteristics of the newly bred alpine rhododendron varieties for the production of Spring Festival flowers are still unclear, which is precisely the issue that domestic alpine rhododendron growers prioritize when selecting alpine rhododendron varieties. To promote the industrial application of newly bred alpine rhododendron varieties in my country, it is of great practical significance to understand the flowering characteristics of these varieties from a technological perspective and to develop a method for screening New Year's rhododendron varieties. This will help address growers' concerns about selection and promote the industrial application of newly bred varieties.

[0004] The existing method for screening alpine rhododendron varieties suitable for the Lunar New Year usually involves using potted plants with flower buds as experimental materials. Flowering time regulation experiments are then conducted to observe their flowering performance, thereby determining their suitability as Lunar New Year varieties (Liu Xiaoqing, Su Jiale, Li Chang, et al. Comparative study on the flowering-inducing effects of alpine rhododendron varieties during the Spring Festival [J]. Jiangsu Agricultural Sciences, 2013, 41(09): 167-168). However, this screening method is not only time-consuming and complex, but it can also damage the plants of varieties unsuitable for Lunar New Year flowering regulation, causing the potted plants to fail to grow normally the following year.

[0005] To overcome the above problems, we need to provide a method for screening alpine rhododendron varieties suitable for the Lunar New Year, reducing damage to potted plants and lowering the difficulty of screening alpine rhododendron varieties suitable for the Lunar New Year. Summary of the Invention

[0006] To overcome the problems existing in the background technology, this application provides a method for screening alpine rhododendron varieties suitable for the Lunar New Year. The method uses branch cutting and cold storage for flower induction and hierarchical analysis to screen suitable alpine rhododendron varieties for the Lunar New Year. The operation is simple and easy to implement, and it does not harm potted flowers, thus reducing the difficulty of screening alpine rhododendron varieties suitable for the Lunar New Year.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solution:

[0008] The method for screening alpine rhododendron varieties for the Lunar New Year using cold-stored flowering of cut branches includes the following specific steps:

[0009] (1) Collection: Select plants, cut flower branches and disinfect them;

[0010] (2) Cultivation: The disinfected flower branches were inserted into clean water and refrigerated in the dark with the potted plants of the positive control and negative control. The flower branches and potted plants were taken out and their refrigeration quality index, namely C1: refrigeration frost damage rate (%), was observed. The flower branches were then inserted into a container with preservative. The flower branches and potted plants were cultivated under light until flowering. The positive control and negative control were 3-year-old alpine rhododendrons with no less than 3 flower buds, namely 'Huayan' and 'Fenjingling'.

[0011] (3) Observation of traits: After cultivation, observe the flowering quality indicators of flower branches and potted plants, namely C2: time required from leaving the warehouse to flowering (d); C3: flowering rate of branches (%); C4: inflorescence opening degree (%); C5: inflorescence size (cm);

[0012] (4) Scoring: Based on the observed indicators, the weight (W) of each indicator was determined by the hierarchical analysis method. The weights of indicators C1, C2, C3, C4 and C5 are W1=0.3660, W2=0.2645, W3=0.1631, W4=0.1331 and W5=0.0733, respectively. Then, each treatment was scored (P) according to the trait grade scoring standard, i.e., Table 1. The score (C) of each indicator was multiplied by the corresponding weight (W) to calculate the comprehensive trait score (P') of each variety.

[0013] Table 1 Grading Criteria for Cutting Characteristics of Alpine Rhododendron

[0014]

[0015] (5) Screening: Varieties whose comprehensive trait score (P') of the flower branches is not significantly different from that of the negative control are determined to be unsuitable for flower induction, while varieties whose comprehensive trait score is not significantly different from that of the positive control are determined to be suitable for flower induction, which are the New Year's Eve type varieties.

[0016] Further optimization is made by the following step (1): the plant is a 2-gallon potted alpine rhododendron that is growing well without pests or diseases, has a growth period of more than 3 years, and has reached the flowering period.

[0017] Further preferred, in step (1), the thickness of the flower branch is 0.60-1.20 cm; the diameter of the flower bud on the flower branch is 1.80-2.10 cm.

[0018] Further preferred, in step (1), the cutting method is as follows: cut the flower branches in October-November, retain only 3-5 leaves below the top of the flower branch, and remove the axillary buds under the petioles of the flower branches and the dormant buds under the flower buds.

[0019] Further preferred, in step (1), the disinfection method is to soak the flower branches in a methyl thiophanate aqueous solution for 30 minutes, wherein the mass concentration of the methyl thiophanate aqueous solution is 1.25-2 g / L.

[0020] Further preferred, in step (2), the temperature of the cold culture is 6-8℃ and the cold culture time is 25-45 days.

[0021] Further preferred, in step (2), the illumination conditions are: illumination intensity of 1500 lx and illumination duration of 12-14 h.

[0022] Further preferred, in step (2), the temperature for cultivation under light conditions is 23-25℃ and the humidity is 70%-90%.

[0023] Further preferred, in step (2), the preservative is 4.50 g / L sucrose + 150 mg / L 8-hydroxyquinoline citrate + 100 mg / L citric acid; the amount of the preservative is such that it submerges the flower stem cut 5 cm above the cut surface.

[0024] Further preferred, in step (2), the amount of clean water used is 100-400 mL.

[0025] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application.

[0026] The beneficial effects of this invention are:

[0027] 1. This application provides a method for screening alpine rhododendron varieties suitable for the Lunar New Year. The method is simple to operate, easy to implement, and does not harm potted plants, thus reducing the difficulty of screening alpine rhododendron varieties suitable for the Lunar New Year. This is because this application uses a branch-cutting and cold-freezing method to induce flowering in alpine rhododendrons. On the one hand, this avoids overall low-temperature cold-freezing of potted alpine rhododendrons, greatly reducing damage to the plants and not affecting their normal growth and development cycle. On the other hand, the experiment can be completed using conventional equipment such as freezers and artificial climate chambers, saving space, reducing operational difficulty, and facilitating large-scale screening and identification of alpine rhododendron varieties suitable for the Lunar New Year.

[0028] 2. This application utilizes the Analytic Hierarchy Process (AHP) to accurately identify and screen alpine rhododendron varieties suitable for the Lunar New Year. For new varieties whose floral characteristics are not yet fully understood, this significantly reduces the management difficulty of flower cultivation, helps address growers' selection concerns, and promotes the industrial application of self-bred new varieties. This is because the AHP, as a systematic and hierarchical multi-criteria analysis method, can decompose complex problems into multiple levels such as objectives and criteria, facilitating systematic analysis. It also comprehensively considers both quantitative and qualitative factors, avoiding the omission of important indicators. The production of alpine rhododendrons for the Lunar New Year requires consideration of multiple factors, including breaking dormancy with low temperatures, inducing flowering with heating, and the cold resistance of the varieties. Traditional methods require step-by-step testing of various potted alpine rhododendron varieties at multiple stages to determine their suitability for inducing flowering. This method is complex, time-consuming, and requires a large area. This application, by combining cold-pressed cuttings for inducing flowering with the AHP, can quickly and accurately screen alpine rhododendron varieties suitable for the Lunar New Year. Attached Figure Description

[0029] Figure 1 This is an illustration of the effect of cold storage and flower induction of alpine rhododendron cuttings in Embodiment 1 of this application;

[0030] Figure 2 This is a diagram illustrating the effect of cold storage and flower-inducing process on potted alpine rhododendrons in Embodiment 1 of this application.

[0031] Figure 3 This is a diagram illustrating the effect of cold storage and flower-inducing of cut rhododendron branches in Embodiment 2 of this application;

[0032] Figure 4 This is a diagram illustrating the effect of cold storage forcing flowering of potted alpine rhododendrons in Embodiment 2 of this application;

[0033] Figure 5 This is a comparative example 1 of the application, showing the effect of cold-frozen flower-inducing process on potted alpine rhododendrons. Detailed Implementation

[0034] To make the objectives, technical solutions, and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below to facilitate understanding by those skilled in the art.

[0035] This invention provides a method for screening alpine rhododendron varieties suitable for the Lunar New Year, which can reduce the difficulty of screening alpine rhododendron varieties suitable for the Lunar New Year and effectively promote the large-scale production of self-bred new varieties suitable for the Lunar New Year.

[0036] Unless otherwise specified, all reagents and materials used in the following examples were purchased from the market.

[0037] Example 1: (A method for screening alpine rhododendron varieties suitable for the Lunar New Year)

[0038] (1) Collection: On October 20, 2023, healthy, disease-free, and flowering rhododendrons with a growth period of more than 3 years were selected in 2-gallon pots. The selected varieties were self-bred varieties 'Huayan', 'Fenjingling', 'Yunzhifeiyu', 'Yunzhirancai', 'Tianxin', and 'Yangchunxue' (the above 6 rhododendron varieties can be obtained by the public from the Flower Research Institute of Yunnan Academy of Agricultural Sciences). 48 flower branches of each of these 6 varieties were cut. The thickness of the cut branches was 0.60 cm and the diameter of the flower buds was 1.80 cm. Excess leaves on the flower branches of each variety were removed, leaving only 3-5 leaves below the top of the flower branch. The axillary buds under the petioles of the flower branches and the dormant buds under the flower buds were removed. The flower branches were soaked in a 2 g / L aqueous solution of methyl thiophanate (produced by Japan Soda Co., Ltd., with an active ingredient of 70%) for 30 minutes. Then the flower branches were taken out and dried in the shade to complete the disinfection treatment.

[0039] (2) Cold storage: Insert the sterilized flower branches into a triangular conical bottle containing 150 ml of clean water and place it in a refrigerator (produced by Zhejiang Xingxing Cold Chain Integration Co., Ltd., model LGC-1500FS3) and cultivate it at 6-8℃ in the dark (i.e., without light) for 40 days. At the same time, 9 pots of 'Huayan' and 'Fenjingling' with no less than 3 flower buds each, which are 3 years old, are used as positive control and negative control respectively and are cold stored under the same conditions.

[0040] (3) Cultivation: On November 28, 2023, the flower branches and potted plants after cold storage cultivation were taken out and their cold storage quality index, namely C1: cold storage frost damage rate (%), was observed. The calculation method is: cold storage frost damage rate (%) = frozen flower branches / total number of treated flower branches × 100%. Each variety of flower branches was inserted into a triangular conical bottle containing preservative, ensuring that the cut of the flower branch was immersed in the preservative for 5 cm. The preservative was: 4.50 g / L sucrose + 150 mg / L 8-hydroxyquinoline citrate + 100 mg / L citric acid. The flower branches and potted plants were cultivated in an artificial climate incubator (manufactured by BINDER, Germany, model KBWF720) with a temperature of 23℃, a light intensity of 1500 lx, a light duration of 14 h, and a humidity of 70%-90% until flowering.

[0041] (4) Observation of traits: After cultivation, observe the flowering quality indicators of cut branches and potted plants: namely C2: time required from leaving storage to flowering (d), C3: flowering rate of branches (%), C4: inflorescence opening degree (%), C5: inflorescence size (cm). The calculation methods of the above indicators are as follows:

[0042] ,

[0043] ,

[0044] ,

[0045] ;

[0046] (5) Scoring: Based on the observed indicators, the weight (W) of each indicator was determined by the analytic hierarchy process (AHP), as detailed in Table 2. Then, each treatment was scored (P) according to the trait grade scoring criteria in Table 1. The score (C) of each indicator was multiplied by the corresponding weight (W) to calculate the comprehensive trait score (P') of each variety.

[0047] Table 2. Weight values ​​of various traits for flower induction by cutting branches of Rhododendron simsii.

[0048]

[0049] (6) Determine the New Year's Day type varieties: Varieties whose comprehensive trait score (P') of cut flower branches is not significantly different from that of the negative control ('Pink Elf' potted plant) are determined to be unsuitable for forced flowering, while varieties whose comprehensive trait score (P') is not significantly different from that of the positive control ('Flower Beauty' potted plant) are determined to be suitable for forced flowering, which are the New Year's Day type varieties.

[0050] Data Analysis:

[0051] From Table 3 and Figure 1 , 2 It can be seen that the comprehensive evaluation scores of the tested varieties are as follows: Huayan bonsai > Huayan > Yunzhifeiyu > Yangchunxue > Yunzhirancai > bonsai pink elf > pink elf = sweetheart. There is no significant difference between the comprehensive evaluation scores of cut branches of varieties such as pink elf and sweetheart and the negative control (i.e., 'pink elf' bonsai). Therefore, 'pink elf' and 'sweetheart' are not suitable for flower-forcing treatment. There is no significant difference between the comprehensive evaluation scores of cut branches of varieties such as Huayan, Yunzhifeiyu, Yangchunxue, and Yunzhirancai and the positive control (i.e., 'huayan' bonsai). Therefore, varieties such as 'huayan', 'Yunzhifeiyu', 'Yangchunxue', and 'Yunzhirancai' are suitable as New Year's varieties.

[0052] Table 3. Comparison of flowering of cut branches from different alpine rhododendron varieties after cold storage.

[0053]

[0054] Note: Data with the same letter for the same trait indicate no significant difference (P>0.05), while data with different letters indicate significant differences (P<0.05).

[0055] Example 2: (A method for screening alpine rhododendron varieties suitable for the Lunar New Year)

[0056] (1) Collection: On October 20, 2023, healthy, disease-free, and flowering rhododendrons with a growth period of more than 3 years were selected. The selected varieties were the self-bred varieties 'Guifei Zuijiu', 'Yihong', 'Baochai', and 'Cuihu Lianxiang' (the above 4 rhododendron varieties can be obtained by the public from the Flower Research Institute of Yunnan Academy of Agricultural Sciences). 48 flower branches of each of these 4 varieties were cut. The thickness of the cut branches was 1.20 cm and the diameter of the flower buds was 1.80 cm. Excess leaves on the flower branches of each variety were removed, leaving only 3-5 leaves below the top of the flower branch. The axillary buds under the petioles of the flower branches and the dormant buds under the flower buds were removed. The flower branches were soaked in a 1.25 g / L aqueous solution of methyl thiophanate (produced by Japan Soda Co., Ltd., with an active ingredient of 70%) for 30 minutes. Then the flower branches were taken out and dried in the shade to complete the disinfection treatment.

[0057] (2) Cold storage: After disinfection, insert a single flower branch into a triangular conical bottle containing 180 ml of clean water and place it in a refrigerator (produced by Zhejiang Xingxing Cold Chain Integration Co., Ltd., model LGC-1500FS3). Refrigerate at 6-8℃ in the dark (i.e., without light) for 35 days. At the same time, 9 pots of 'Huayan' and 'Fenjingling' with no less than 3 flower buds each, which are 3 years old, are used as positive and negative controls and refrigerated under the same conditions.

[0058] (3) Cultivation: On November 23, 2023, the flower branches and potted plants after cold storage cultivation were taken out and their cold storage quality index, namely C1: cold storage frost damage rate (%), was observed. The calculation method is: cold storage frost damage rate (%) = frozen flower branches / total number of treated flower branches × 100%. Each variety of flower branches was inserted into a triangular conical bottle containing preservative, ensuring that the cut of the flower branch was immersed in the preservative for 5 cm. The preservative was: 4.50 g / L sucrose + 150 mg / L 8-hydroxyquinoline citrate + 100 mg / L citric acid. The flower branches and potted plants were cultivated in an artificial climate incubator (manufactured by BINDER, Germany, model KBWF720) with a temperature of 23℃, a light intensity of 1500 lx, a light duration of 14 h, and a humidity of 70%-90% until flowering.

[0059] (4) Observation of traits: After cultivation, observe the flowering quality indicators of cut branches and potted plants: namely C2: time required from leaving the warehouse to flowering (d), C3: flowering rate of branches (%), C4: inflorescence opening degree (%), C5: inflorescence size (cm);

[0060] (5) Trait grade scoring: Based on the observed indicators, the weight (W) of each indicator was determined by the analytic hierarchy process (AHP), as detailed in Table 2. Then, each treatment was scored (P) according to the trait grade scoring criteria in Table 1. The score (C) of each indicator was multiplied by the corresponding weight (W) to calculate the comprehensive trait score (P') of each variety.

[0061] (6) Determine the New Year's Day type varieties: Varieties whose comprehensive trait score (P') of cut flower branches is not significantly different from that of the negative control ('Pink Elf' potted plant) are determined to be unsuitable for forced flowering, while varieties whose comprehensive trait score (P') is not significantly different from that of the positive control ('Flower Beauty' potted plant) are determined to be suitable for forced flowering, which are the New Year's Day type varieties.

[0062] From Table 4 and Figure 3 , 4 It can be seen that the comprehensive evaluation scores of the tested varieties are as follows: potted Huayan > Guifei Zuijiu = Cuihu Lianxiang > Baochai > Yihong > potted Fenjingling. There is no significant difference between the comprehensive evaluation scores of cut branches of varieties such as 'Baochai' and 'Yihong' and the negative control (i.e., 'Fenjingling' potted plants). Therefore, 'Baochai' and 'Yihong' are not suitable for flower-forcing treatment. There is no significant difference between the comprehensive evaluation scores of cut branches of varieties such as 'Guifei Zuijiu' and 'Cuihu Lianxiang' and the positive control (i.e., 'Huayan' potted plants). Therefore, 'Guifei Zuijiu' and 'Cuihu Lianxiang' are suitable as New Year's Eve varieties.

[0063] Table 4. Comparison of flowering of cut branches from different alpine rhododendron varieties after cold storage

[0064]

[0065] Note: Data with the same letter for the same trait indicate no significant difference (P>0.05), while data with different letters indicate significant differences (P<0.05).

[0066] Comparative Example 1: (An example of cold-induced flowering of potted rhododendrons of other tested varieties)

[0067] (1) Except for replacing the plant material with alpine rhododendron bonsai of 3-year-old varieties with no less than 3 flower buds such as 'Yangchunxue', 'Baochai', 'Yunzhifeiyu', 'Tianxin', and 'Cuihulianxiang', 9 pots of each variety are selected and no branch cutting is performed, all other steps are the same as in Example 1.

[0068] According to Table 5 and Figure 5It can be seen that the potted varieties *Yangchunxue* and *Yunzhifeiyu* showed the best effect in flower induction through cold storage, with zero frost damage and leaf drop rates. The time required from removal from storage to flowering was 38.21 days and 33.06 days, respectively, and all flower buds on the branches were able to bloom, thus proving that they are suitable for flower induction. Although the potted variety *Cuihulianxiang* had an 8.21% frost damage rate and a 6.45% leaf drop rate, it also achieved 100% flowering, thus proving that it is also suitable for flower induction. However, the potted varieties *Baochai* and *Tianxin* suffered severe leaf drop during cold storage, with leaf drop rates reaching 78.28% and 53.41%, respectively, and frost damage rates of 91.45% and 76.19%, respectively, seriously affecting their ornamental value. Furthermore, the flower buds of these two alpine rhododendron varieties became stiff and failed to open after cold storage, resulting in only 39.71% of the branches of *Tianxin* flowering, and its inflorescence opening rate being only 29.52%. The buds of Baochai failed to open at all, and some potted plants with severe leaf drop even withered and died. These data indicate that Baochai and Tianxin are not suitable for cold-frozen forcing to bloom and are not suitable as alpine rhododendron varieties for the Lunar New Year.

[0069] The results of Comparative Example 1 show that potted varieties such as Yangchunxue, Yunzhifeiyu, and Cuihulianxiang can be induced to bloom and are suitable as New Year's varieties, while potted varieties such as Baochai and Tianxin are not suitable for induced blooming. This is consistent with the final results of Examples 1 and 2. These data further demonstrate that the method provided in this application for screening alpine rhododendron New Year's varieties using cold-stored flowering of cut branches is relatively accurate.

[0070] Table 5. Flowering of potted rhododendron varieties after cold storage

[0071]

[0072] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A method for screening alpine rhododendron varieties suitable for the Lunar New Year, characterized in that, The method includes the following steps: (1) Collection: Select healthy, disease-free, and flowering rhododendron plants in 2-gallon pots that have been growing for more than 3 years. Cut the flower branches in October-November, keeping only 3-5 leaves below the top of the flower branch. Remove the axillary buds below the petioles of the flower branches and the dormant buds below the flower buds and disinfect them. The thickness of the flower branches is 0.60-1.20 cm and the diameter of the flower buds on the flower branches is 1.80-2.10 cm. (2) Cultivation: After disinfection, the flower branches were inserted into clean water and refrigerated at 6-8℃ in the dark for 25-45 days, along with the potted plants of the positive control and negative control. The flower branches and potted plants were taken out and their refrigeration quality index, namely C1: refrigeration frost damage rate (%), was observed. The flower branches were then inserted into a container with preservative. The flower branches and potted plants were cultivated under light conditions until flowering. The positive control and negative control were 3-year-old alpine rhododendrons with no less than 3 flower buds, namely 'Huayan' and 'Fenjingling'. (3) Observation of traits: After cultivation, observe the flowering quality indicators of flower branches and potted plants, namely C2: time required from leaving the warehouse to flowering (d); C3: flowering rate of branches (%); C4: inflorescence opening degree (%); C5: inflorescence size (cm); (4) Scoring: Based on the observed indicators, the weight (W) of each indicator was determined by the hierarchical analysis method. The weights of indicators C1, C2, C3, C4 and C5 are W1=0.3660, W2=0.2645, W3=0.1631, W4=0.1331 and W5=0.0733, respectively. Then, each treatment was scored (P) according to the trait grade scoring standard, i.e., Table 1. The score (C) of each indicator was multiplied by the corresponding weight (W) to calculate the comprehensive trait score (P') of each variety. Table 1 Grading Criteria for Cutting Characteristics of Alpine Rhododendron (5) Screening: Varieties whose comprehensive trait score (P') of the flower branches is not significantly different from that of the negative control are determined to be unsuitable for flower induction, while varieties whose comprehensive trait score is not significantly different from that of the positive control are determined to be suitable for flower induction, which are the New Year's Eve type varieties.

2. The method according to claim 1, characterized in that, In step (1), the disinfection method is to soak the flower branches in a methyl thiophanate aqueous solution for 30 minutes, wherein the mass concentration of the methyl thiophanate aqueous solution is 1.25-2 g / L.

3. The method according to claim 1, characterized in that, In step (2), the illumination conditions are: illumination intensity of 1500 lx and illumination duration of 12-14 h.

4. The method according to claim 1, characterized in that, In step (2), the temperature for cultivation under light conditions is 23-25℃ and the humidity is 70%-90%.

5. The method according to claim 1, characterized in that, In step (2), the preservative is 4.50 g / L sucrose + 150 mg / L 8-hydroxyquinoline citrate + 100 mg / L citric acid; the amount of the preservative is such that it submerges the cut of the flower branch by 5 cm.

6. The method according to claim 1, characterized in that, In step (2), the amount of clean water used is 100-400 mL.

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