A foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables.

Abstract

This invention relates to the field of foliar fertilizer technology, and more particularly to a foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables. The foliar fertilizer additive provided by this invention, containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables, comprises the following raw materials in parts by weight: 20-30 parts sulfur-rich organic matter, 5-10 parts tea polyphenols, 4-7 parts plant growth regulators, 10-14 parts slow-release agents, 15-18 parts bentonite, and 4-8 parts seaweed extract. With sulfur-rich organic matter as the core component, this additive aims to provide plants with essential sulfur-containing amino acids, which play important roles in various physiological processes within plants, participating in protein and vitamin synthesis. Combined with multiple functional components such as tea polyphenols, plant growth regulators, and slow-release agents, it can comprehensively optimize plant health, increase the amino acid and vitamin content in fruits and vegetables, and ultimately achieve the goal of enhancing the nutritional value of fruits and vegetables.

Description

Technical Field

[0001] This invention relates to the field of foliar fertilizer technology, and in particular to a foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables. Background Technology

[0002] With rising economic levels and increased health awareness, consumer demand for fruits and vegetables has shifted from simply pursuing high yields to a dual focus on nutritional quality and flavor characteristics. Researchers are continuously dedicated to developing various foliar fertilizers and additives. However, traditional foliar fertilizers mainly focus on providing the basic nutrients (such as nitrogen, phosphorus, and potassium) and some micronutrients needed for plant growth. While these fertilizers help with basic crop growth, their effectiveness in improving the nutritional value of crops is limited.

[0003] Traditional foliar fertilizers are difficult to effectively regulate the accumulation of bioactive substances such as amino acids, vitamins, and polyphenols in fruits, neglect the effective regulation of key metabolic pathways in plants, and can lead to an imbalance in the plant's foliar microbial community due to excessive application, indirectly affecting the synthesis of secondary metabolites.

[0004] Most foliar fertilizers on the market are not specifically designed to enhance the synthesis and accumulation of specific beneficial compounds in fruits and vegetables, such as increasing amino acid and vitamin content. This poses a challenge to meeting consumers' demand for high-nutritional-value foods. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a foliar fertilizer additive containing sulfur-rich organic matter for improving the nutritional value of fruits and vegetables, which solves the technical problem that it is difficult to effectively improve the nutritional value and taste of fruits and vegetables in the prior art.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the main technical solutions adopted in this invention include: foliar fertilizer additives containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables, preparation methods, and applications.

[0009] Foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables includes the following raw materials in parts by weight: sulfur-rich organic matter: 20-30 parts, tea polyphenols: 5-10 parts, growth regulators: 4-7 parts, slow-release agents: 10-14 parts, bentonite: 15-18 parts, and seaweed extract: 4-8 parts.

[0010] Sulfur-rich organic matter is a core component of additives, and amino acids are the basic building blocks of proteins. Sulfur-rich amino acids, such as cysteine, methionine, and taurine, contain sulfur. These amino acids participate in various physiological processes in plants, including protein synthesis, and help promote the accumulation of soluble sugars and organic acids in fruits and vegetables, thereby improving fruit texture, nutritional value, and overall yield. These key amino acids help plants synthesize proteins more efficiently, thus increasing the protein content and quality of fruits. Providing essential amino acids helps strengthen the structure and texture of fruits and vegetables, making them firmer and more elastic. Furthermore, sulfur-containing compounds can also affect the flavor of fruits and vegetables, adding unique aromas and tastes, and enhancing the overall eating experience.

[0011] Carbohydrates not only provide energy for plants, but also improve soil structure, increase soil water retention and aeration, which is beneficial to root growth and nutrient absorption, and thus affects the quality and nutritional value of fruits and vegetables.

[0012] Lipids are a major component of biological membranes and are essential for maintaining cell membrane integrity and function. Healthy cell membranes help plants better cope with environmental changes and improve the efficiency of water and nutrient transport, thereby supporting the overall health and productivity of plants. Good plant health typically leads to higher yields and better quality, including increased levels of vitamins, minerals, and other beneficial compounds.

[0013] This invention provides a foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables. It is formulated from sulfur-rich organic matter, tea polyphenols, growth regulators, slow-release agents, bentonite, and seaweed extracts in specific proportions. This additive promotes the synthesis of proteins and vitamins in plants by providing sulfur-rich amino acids, effectively improving the nutritional value and taste of fruits and vegetables. Simultaneously, combined with growth regulators and slow-release technology, it enhances plant resistance to stress and nutrient utilization efficiency, prolongs the fertilizer's effect, and reduces the frequency of fertilization, resulting in a comprehensive effect of safety, environmental friendliness, and increased yield and quality.

[0014] Tea polyphenols possess significant antioxidant properties, capable of scavenging free radicals within plants. This helps protect the active ingredients in sulfur-rich organic matter from oxidative degradation, ensuring their better participation in plant metabolic processes, including protein synthesis and the construction of antioxidant defense systems.

[0015] Plant growth regulators can regulate plant growth and development, including promoting root growth, enhancing photosynthetic efficiency, and improving nutrient absorption. They optimize the overall growth status of plants, enabling them to more effectively utilize nutrients such as amino acids provided by sulfur-rich organic matter, thereby increasing nutritional value and yield.

[0016] As a carrier material, bentonite possesses excellent adsorption and permeability, helping to evenly distribute and slowly release various components in additives, including sulfur-rich organic matter. Bentonite also improves soil structure, increases soil aeration and water retention capacity, and facilitates root absorption of water and nutrients, indirectly supporting the effectiveness of sulfur-rich organic matter.

[0017] Seaweed extract is rich in various minerals, vitamins, plant hormones, and other bioactive substances, which can stimulate the plant's immune response, enhance its resistance to diseases and pests, and promote plant growth and development. These properties help create a healthier growing environment, enabling plants to fully utilize the nutrients provided by sulfur-rich organic matter, further improving the nutritional value and quality of fruits and vegetables.

[0018] In some embodiments, sulfur-rich organic matter includes sulfur-rich proteins, which include sulfur-rich amino acids and other amino acids in a weight ratio of 35%-60%:40-65%; the other amino acids include cysteine, methionine, and taurine; the remaining amino acids include histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, valine, alanine, arginine, aspartic acid, glycine, glutamic acid, and serine.

[0019] By combining sulfur-rich amino acids with other essential amino acids, this additive significantly enhances the nutritional value and taste of fruits and vegetables. Sulfur-rich amino acids help promote the synthesis of various bioactive substances such as glutathione and vitamins in plants, while also improving their flavor and texture. Furthermore, the rationally proportioned amino acid mixture effectively promotes the efficiency of nutrient absorption and utilization by plants, increasing the content of vitamins, minerals, and other beneficial components in fruits and vegetables, thus providing richer nutritional value. This not only improves the overall quality of fruits and vegetables but also makes them more delicious, meeting consumers' demand for high-quality agricultural products.

[0020] In some embodiments, the weight percentages of cysteine, methionine, and taurine in the sulfur-rich amino acids are: 30%-40%: 40%-45%: 15%-30%.

[0021] By combining cysteine, methionine, and taurine in a specific ratio to form sulfur-rich organic matter, the synthesis and accumulation of nutrients in plants can be effectively promoted, significantly enhancing the nutritional value and taste of fruits and vegetables. This sulfur-rich amino acid system helps optimize the formation of flavor compounds in fruits, increasing sugar content, vitamin content, and total amino acid content, making fruits and vegetables more delicious and palatable.

[0022] In some embodiments, sulfur-rich organic matter also includes carbohydrates, lipids, and other substances, with the weight ratio of sulfur-rich protein, carbohydrates, lipids, and other substances being 65-80%: 5-10%: 10-20%: 5-10%.

[0023] In some embodiments, the growth regulator includes choline chloride and brassinolide, wherein choline chloride is 2-4 parts and brassinolide is 2-3 parts; the sustained-release agent includes polyvinyl alcohol and polymalic acid, wherein polyvinyl alcohol is 6-8 parts and polymalic acid is 4-6 parts.

[0024] A mixture of 2-4 parts choline chloride and 2-3 parts brassinolide in this ratio can promote plant cell metabolism and enhance stress resistance. It can stimulate the growth potential of crops without causing physiological disorders due to excessive stimulation. This ratio helps to improve the transport and absorption efficiency of sulfur-rich amino acids and other nutrients in the body, achieving an organic unity of nutrition and regulation.

[0025] In the slow-release system, 6-8 parts of polyvinyl alcohol serve as a hydrophobic coating material, effectively delaying nutrient release, while 4-6 parts of polymalic acid, through its excellent water retention and complexing properties, further stabilize the release curve and improve the soil microenvironment. At this ratio, the slow-release performance of the fertilizer is optimized, enhancing the stability and utilization rate of bioactive components, and forming a highly efficient, long-lasting, and safe comprehensive regulatory system with sulfur-rich organic matter and other functional components.

[0026] In some embodiments, tea polyphenols are preferably 6-9 parts, and the growth regulator is preferably 5-6 parts. The ratio of 6-9 parts tea polyphenols, 5-6 parts growth regulator (choline chloride and brassinolide, in total), and sulfur-rich organic matter in this invention is designed based on their effects on crop nutrition and taste improvement. At a ratio of 6-9 parts, tea polyphenols can fully exert their antioxidant properties, protecting sulfur-containing amino acids in sulfur-rich organic matter from oxidative degradation and enhancing the activity of antioxidant enzyme systems in plants. This ratio ensures sufficient antioxidant capacity to support the overall health of the plant. When the growth regulator is added at a ratio of 5-6 parts, it can optimally regulate plant metabolism, promote root development, and improve nutrient absorption. This enhances absorption efficiency and activates the synthesis and transport of sulfur-containing amino acids and other key substances, thereby further improving the plant's utilization efficiency of nutrients. Sulfur-rich organic matter, as a core functional component, provides abundant sulfur-based amino acids, which are crucial for protein synthesis and plant growth. The combination of these three components in the aforementioned proportions strengthens the plant's antioxidant defense system, optimizes nutrient absorption and utilization efficiency, promotes the accumulation of sugars, vitamins, and amino acids, and ultimately significantly improves the nutritional value and taste quality of fruits and vegetables. This precise ratio ensures complementary functions and synergistic effects among the components.

[0027] In some embodiments, the sustained-release agent is 11-13 parts and the bentonite is 16-17 parts.

[0028] A stable, controllable, and long-lasting functional foliar fertilizer system is formed by combining 11-13 parts of sulfur-rich organic matter, a slow-release agent, and 16-17 parts of bentonite. Sulfur-rich organic matter, as the core nutrient source, is rich in sulfur-containing amino acids such as cysteine, methionine, and taurine, as well as various essential amino acids. It can significantly enhance the protein synthesis capacity and antioxidant levels in plants, thereby increasing the nutritional value of fruits and vegetables. The slow-release agent, composed of polyvinyl alcohol and polymalic acid, effectively regulates the release rate of active ingredients at a ratio of 11-13 parts, preventing rapid nutrient loss, extending the fertilizer's effective period, and improving utilization. Bentonite, at an addition of 16-17 parts, plays a good role in adsorption and structural support, not only enhancing the product's physical stability and dispersibility but also promoting the uniform binding and adhesion of the slow-release agent and sulfur-rich organic matter. Through the combined action of these three components, a stable and slow-release supply of nutrients is achieved, along with efficient absorption. This significantly increases the accumulation of key nutrients such as amino acids, vitamins, and soluble sugars in fruits and vegetables, while also improving fruit flavor and taste.

[0029] In some embodiments, seaweed extract: 5-7 parts, tea polyphenols: 6-9 parts.

[0030] At this ratio, the interaction between seaweed extract and tea polyphenols activates the enzyme system in the plant, promotes the absorption and conversion of nutrients such as nitrogen and sulfur, and improves the synthesis efficiency of sulfur-rich amino acids, thereby enhancing protein content, amino acid balance, and vitamin synthesis. Tea polyphenols effectively protect the active substances in seaweed extract from oxidative degradation, prolong their action time in the plant, and improve bioavailability.

[0031] This combination can also complement the controlled-release system formed by slow-release agents and bentonite: slow-release agents regulate the nutrient release rhythm, and bentonite enhances adsorption and dispersibility, so that the functional components of seaweed extract and tea polyphenols can act more evenly and for a longer period of time on the crop surface, thereby achieving optimization of the entire process from nutrient supply to metabolic regulation.

[0032] The synergistic effect of seaweed extract and tea polyphenols in the range of 5-7 parts and 6-9 parts not only enhances the plant's resistance to stress, but also promotes the accumulation of key nutrients such as soluble sugars, vitamins, and flavor amino acids in fruits and vegetables, significantly improving the color, sweetness, and taste of the fruit.

[0033] In some embodiments, the preparation process of sulfur-rich organic matter is as follows:

[0034] Step 1, use 10 m 3 The microbial protein reactor was maintained at a temperature of 30 °C; mineral salt culture medium was continuously supplied via a peristaltic pump, and the hydraulic retention time was set to 2.5 days.

[0035] A mixture of gases with volume percentages of 15% CO2, 25% O2, 59% H2 and 1% H2S is introduced into the reactor, with a total gas flow rate of 8000 L / d. The gas is thoroughly mixed with the culture medium by an air pump and then injected into the microbial protein reactor through a nozzle. The microbial protein reactor is a spray type.

[0036] Step 2: Add HOB-SOB mixed microbial community and other auxiliary microbial species to the microbial protein reactor to form a complex microbial system. The amount of the complex microbial system added is 10% of the volume of the microbial protein reactor.

[0037] Step 3: Collect bacterial cells every 14 days during the cultivation process, for a total of 7 collections. Centrifuge the collected bacterial cells each time and freeze-dry them to obtain protein-rich microbial biomass products.

[0038] In some embodiments, a 0.2M phosphate buffer solution is used to maintain the pH of the culture medium at 7.0; the culture medium formulation includes the following components: 0.7-1.0 parts of MgCl2·6H2O, 1.0-1.3 parts of NH4Cl, 1.1-1.5 parts of KH2PO4, 1.6-1.8 parts of Na2HPO4, and 0.1-0.5 parts of CaCl2·2H2O.

[0039] This culture medium, through a scientifically formulated blend of various mineral elements and a 0.2 M phosphate buffer system to maintain a stable pH of 7.0, provides a suitable nutritional environment and physiological conditions for the efficient growth and metabolism of the HOB-SOB mixed microbial community. The rational combination of key elements such as magnesium, phosphorus, nitrogen, and calcium not only meets the basic mineral nutritional needs of microorganisms but also promotes the effective utilization of H2S, enhancing the yield and quality of single-cell proteins. Furthermore, this culture medium exhibits good adaptability and stability, capable of adapting to different nitrogen and sulfur supply modes at different operational stages. It plays a crucial supporting role in achieving the simultaneous recovery and conversion of carbon, nitrogen, and sulfur resources, demonstrating promising engineering application prospects and industrialization potential.

[0040] In some embodiments, the composite microbial system includes HOB, SOB, and other auxiliary strains. A 10% volume fraction of a HOB-SOB mixed microbial community is added to the microbial protein reactor, wherein the HOB is selected from... Pseudmonas, Hydrogenophaga, Xanthobacter, Plesiomonas, Prothomonas, Leminobacter, Nocardia, Corynebacter, Rarobacter, Spirilla, Terrabacte, Geobacillus, Flavobacterium, Fattybacilli, Paracoccus, Bacillus, Aeromonas, Micrococcus, Variovorax, Mycobacterium and Alcaligenes One or more of the genus SOB. SOB is selected from Thiobacillus, Acidithiobacillus, Sulfurovum, Sulfurimonas, Halothiobacillus, Thiomonas and Thiovirga One or more of the genera. Other auxiliary strains are selected from... Nocardia, Burkholdefia, Sulfuriferula and ThioalkalivibrioOne or more of the genera. The relative abundance of HOB is 30%-40%, the relative abundance of SOB is 40%-50%, and the relative abundance of other auxiliary species is 10%-15%.

[0041] This invention constructs by Hydrogenophaga and Thiobacillus A complex microbial system with the main component and supplemented by a variety of functionally complementary strains. (Xanthobacter, Plesiomonas, Acidithiobacillus, Sulfurovum, Halothiobacillus) This study achieves highly efficient conversion of CO2 and H2S. HOB, as an autotrophic microorganism, can synthesize microbial proteins using H2, CO2, and O2, while SOB utilizes H2S as an energy source. This not only reduces sulfide toxicity but also provides the essential sulfur element for microbial protein synthesis. Therefore, the composite microbial system composed of HOB and SOB is more stable and conducive to the stable production of microbial proteins. Other auxiliary strains exhibit good ecological adaptability and potential metabolic interactions, contributing to enhanced system stability and improved nutrient utilization. This composite microbial community structure balances functionality and ecological diversity, maintaining a stable metabolic state under complex operating conditions, significantly improving the yield and nutritional value of single-cell microbial proteins, and possessing good engineering application potential and industrialization prospects. In the preparation of sulfur-rich organic matter, HOB and SOB exhibit significant synergistic effects through complementary metabolic functions and ecological interactions. HOB mainly utilizes H2 and CO2 for autotrophic growth, fixing carbon sources to synthesize microbial proteins; SOB uses H2S as an energy source for oxidative metabolism, converting it into sulfate culture medium components. This division of labor not only effectively reduced the toxicity of H2S in the system and ensured the stability of the microbial community, but also achieved the simultaneous recovery and conversion of carbon and sulfur resources. Hydrogenophaga and Thiobacillus HOBs and SOBs, represented by these microorganisms, dominate the complex microbial community and form a synergistic metabolic network with other helper microorganisms, further enhancing nutrient utilization and system resilience. This synergistic mechanism provides a solid foundation for the efficient production of protein-rich microbial biomass.

[0042] Secondly, this application provides a method for preparing a foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables, comprising the following steps:

[0043] Step S1: Prepare a 10% aqueous solution of 2-4 parts choline chloride and a 5% ethanol solution of 2-3 parts brassinolide. Mix the choline chloride solution and the brassinolide solution evenly.

[0044] Step S2

[0045] Mix 20–30 parts of sulfur-rich organic matter, 5–10 parts of tea polyphenols, 15–18 parts of bentonite and 4–8 parts of seaweed extract, and pulverize with an air jet mill for 15–20 minutes to make the particle size of the mixed powder less than 200 mesh.

[0046] Step S3

[0047] Add 30-50 parts of deionized water and 5-10 parts of ethanol to the above-mentioned pulverized mixture, then transfer it to a high-speed homogenizer and shear and stir at 5000-7000 rpm for 10-15 minutes to fully wet and initially disperse the components.

[0048] Step S4

[0049] Slowly add the mixed solution of choline chloride and brassinolide prepared in step S1 to the dispersion system in step S3, and keep the stirring speed at 300-500 rpm for 5-10 minutes to make the growth regulator evenly distributed in the system; then let the mixed system stand for 2 hours to allow the active ingredients to fully bind to the sulfur-rich organic carrier.

[0050] Step S5

[0051] Add 6–8 parts of polyvinyl alcohol and 4–6 parts of polymalic acid to the suspension in step S4 in sequence, and continue stirring at 300–500 rpm for 5–10 minutes to ensure uniform dispersion of the slow-release agent; then add 0.5–1 parts of 10% hydroxypropyl methylcellulose aqueous solution to prevent particle sedimentation and enhance system stability.

[0052] Step S6

[0053] According to the system's flowability requirements, add an appropriate amount of deionized water or 10% hydroxypropyl methylcellulose aqueous solution to adjust the viscosity to between 40-80 mPa·s, and at the same time adjust the pH value of the system to between 6 and 7; after filtering the final uniform suspension through a filter screen to remove impurities, fill it into a light-proof container and seal it for storage.

[0054] In step S1, choline chloride is prepared into a 10% aqueous solution, and brassinolide is prepared into a 5% ethanol solution. These are then mixed thoroughly and allowed to stand for 30 minutes. The interaction of these two solutions activates the plant's growth and metabolic system. This step not only enhances the plant's efficiency in nutrient absorption and transport but also improves the stability and slow-release properties of the active ingredients, extending their duration of action on the leaf surface.

[0055] In step S2, the uniformity and specific surface area of ​​each component are significantly improved, enhancing their dispersibility and the release efficiency of active ingredients in the subsequent system. Ultrafine processing helps improve the adhesion and penetration of additives on leaf surfaces, promoting the effective absorption of amino acids and bioactive components by plants.

[0056] In step S3, high shear force breaks down the agglomeration structure between materials, improving the uniformity and stability of the system. Simultaneously, it promotes the release and dissolution of active ingredients, laying a good foundation for the subsequent addition of growth regulators and sustained-release agents. Furthermore, the addition of an appropriate amount of ethanol helps enhance the solubility of components such as tea polyphenols and provides a certain degree of antibacterial effect, further ensuring product quality and storage stability.

[0057] In step S4, the effective integration of growth regulators and functional components is ensured, improving their stability and bioavailability in the system, while creating favorable conditions for the subsequent introduction of sustained-release agents, thereby enhancing the overall efficacy and durability of the additive.

[0058] In step S5, polyvinyl alcohol, as a hydrophobic coating material, synergistically works with polymalic acid, which has water-retaining and complexing capabilities, to construct a stable slow-release structure, prolonging the release time of the active ingredient and improving fertilizer utilization efficiency. Subsequently, 0.5–1 part of a 10% hydroxypropyl methylcellulose aqueous solution is added to further enhance the rheological and physical stability of the system, prevent particle sedimentation, and ensure the uniformity and consistency of the product during storage and use.

[0059] In step S6, the physical stability and rheological properties of the additives are optimized to ensure that they remain uniform during storage and application; suitable viscosity helps to improve the adhesion and permeability of the product on the leaf surface, and enhances the absorption efficiency of the active ingredients by plants; precise control of pH value helps to maintain the stability and functionality of bioactive substances.

[0060] The foliar fertilizer additive of this application is ultimately in the form of a suspension. The suspension can effectively accommodate poorly soluble but highly functional solid components such as sulfur-rich organic matter, bentonite, and tea polyphenols, ensuring their uniform dispersion and slow-release effect within the system. Simultaneously, plant growth regulators such as choline chloride and brassinolide can be stably loaded onto the surface or interior of the carrier, preventing premature inactivation and enhancing their bioavailability. The added adjuvants, such as polyvinyl alcohol, polymalic acid, and hydroxypropyl methylcellulose, not only improve the rheological and physical stability of the system and prevent particle sedimentation and agglomeration, but also ensure the uniformity and ease of use of the product during storage and application. Furthermore, the suspension system facilitates the absorption of active ingredients through contact with the leaf surface, improving the photosynthetic efficiency and nutrient accumulation levels of fruits and vegetables.

[0061] The foliar fertilizer additive preparation method provided in this application significantly improves the stability, bioactivity, and application effect on fruits and vegetables by scientifically combining sulfur-rich organic matter, growth regulators, and various functional components, and employing a stepwise mixing and slow-release molding process. Choline chloride and brassinolide, activated and compounded at specific concentrations, synergistically regulate plant growth metabolism, enhance photosynthetic efficiency, and promote nutrient accumulation. The sulfur-rich organic matter is rich in functional amino acids such as cysteine, methionine, and taurine, which, combined with tea polyphenols and seaweed extracts, effectively enhance the antioxidant capacity and nutritional value of fruits and vegetables. Bentonite, as an excellent carrier, enhances the product's structural stability and dispersibility. The slow-release system composed of polyvinyl alcohol and polymalic acid ensures the sustained release of active ingredients on the leaves, improving utilization and reducing loss. The entire preparation process is mild and controllable, avoiding high-temperature damage to active ingredients. The granular additive prepared at temperatures not exceeding 40℃ exhibits good solubility, adhesion, and biocompatibility, facilitating storage and application. The foliar fertilizer additive prepared by this method can not only significantly improve the quality and flavor of fruits and vegetables, but also has the advantages of being green, safe, and environmentally friendly, making it suitable for the green production needs of high-quality agricultural products.

[0062] Thirdly, the aforementioned foliar fertilizer additives containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables are applied in foliar fertilizers.

[0063] (III) Beneficial Effects

[0064] This invention provides a foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables. The additive comprises the following raw materials in parts by weight: sulfur-rich organic matter: 20-30 parts; tea polyphenols: 5-10 parts; growth regulators: 4-7 parts; slow-release agents: 10-14 parts; bentonite: 15-18 parts; and seaweed extract: 4-8 parts. By introducing sulfur-rich organic matter as a core component, this additive aims to provide plants with essential sulfur-containing amino acids, which play important roles in various physiological processes within plants, such as protein synthesis and vitamin synthesis. Furthermore, by combining multiple functional components such as tea polyphenols, growth regulators, slow-release agents, bentonite, and seaweed extract, it can comprehensively optimize plant health, enhance disease resistance and environmental adaptability, increase the vitamin and amino acid content in fruits and vegetables, and ultimately achieve the goal of improving the nutritional value of fruits and vegetables. Detailed Implementation

[0065] To facilitate understanding of the present invention, the technical solutions described below are further illustrated with specific embodiments, but the present invention is not limited thereto. All technologies implemented based on the above-described content of the present invention are covered within the scope of protection intended by the present invention. Unless otherwise stated, the raw materials, reagents, and bacterial cultures used in the embodiments are commercially available products. Reagents, instruments, or operating procedures not described herein are all matters that can be conventionally determined by those skilled in the art.

[0066] The number or percentage of portions in the examples are all weight percentages.

[0067] Example 1

[0068] The preparation method of sulfur-rich organic matter includes the following steps:

[0069] Step 1

[0070] Use 10 m 3 The microbial protein reactor was maintained at a temperature of 30 °C.

[0071] The mineral salt culture medium was continuously supplied via a peristaltic pump, with a hydraulic retention time set at 2.5 days. The types and concentrations of the culture medium are as follows:

[0072] The pH of the culture medium was maintained at 7.0 using 0.2M phosphate buffer solution. The AMS culture medium formulation consisted of the following components: 0.7 parts MgCl2·6H2O, 1.1 parts KH2PO4, 1.0 parts NH4Cl, 1.6 parts Na2HPO4, and 0.1 parts CaCl2·2H2O.

[0073] A mixture of gases with volume percentages of 15% CO2, 25% O2, 59% H2 and 1% H2S was introduced into the reactor, with a total gas flow rate of 8000 L / d.

[0074] The gas is thoroughly mixed with the culture medium by an air pump and then injected into the spray-type microbial protein reactor through a nozzle.

[0075] Step 2

[0076] A 10% (v / v) HOB-SOB mixed microbial community was added to the microbial protein reactor, wherein HOB was selected from... Hydrogenophaga Genus; SOB is selected from Thiobacillus Belongs to.

[0077] Build with Hydrogenophaga and Thiobacillus A complex microbial system, primarily composed of microbial strains supplemented with various functionally complementary strains, was developed to achieve efficient conversion of H2S.

[0078] The relative abundance of the bacterial community is shown in Table 1.

[0079] Table 1: Distribution of species and relative abundance of HOB, SOB and other microbial species in the microbial protein reactor in Example 1.

[0080]

[0081] Step 3

[0082] Centrifuge at 10,000 rpm for 10 minutes, and collect the cultured cells every 14 days as an experimental phase, for a total of 10 times. Then freeze-dry the cells to obtain sulfur-rich organic matter.

[0083] The amount of biomass collected is shown in Table 2.

[0084] Table 2: Biomass yield (mgVSS / L) at each experimental stage in the microbial protein reactor in Example 1.

[0085]

[0086] The trend of biomass yield in the microbial protein reactor shows an initial increase followed by a gradual decline. Specifically, it peaks between stages 1 and 3, and although it generally declines thereafter, it exhibits a steep drop in stage 8.

[0087] This is because in the early stages of fermentation, the initial nutrient concentration in the culture medium is high, providing abundant growth conditions and promoting rapid microbial proliferation, leading to an increase in biomass yield. As time progresses, even with continuous replenishment of new nutrients, competition within the microbial community intensifies, potentially preventing some microorganisms from obtaining sufficient resources for effective proliferation, thus affecting overall biomass yield. Microorganisms produce byproducts during metabolism, which inhibit the microorganisms themselves. The inability to promptly remove or transform metabolic products leads to a sharp decline in the eighth stage. However, the first seven stages exhibit relative stability and high biomass yield; therefore, the subsequent synthesis of biomass as a raw material additive is timed to the first through seventh stages.

[0088] The content distribution of each component in biomass at each stage is shown in Table 3.

[0089] Table 3: Content distribution (%) of major components in sulfur-rich organic matter at each experimental stage in Example 1.

[0090]

[0091] Sulfur-rich proteins are the sum of all amino acids.

[0092] In stages 1 through 7, the proportion of sulfur-rich proteins gradually decreases from 80% to 65%. As fermentation progresses, the accumulation of metabolites inhibits the microorganisms' ability to synthesize specific proteins. Furthermore, the interactions between different strains within the complex microbial system change as fermentation progresses. (Main functional strains...) Hydrogenophaga and ThiobacillusThe proportion of microorganisms and their relationship with other auxiliary microorganisms changes, affecting the stability and efficiency of the entire system. Further analysis of abundance changes among each microorganism was conducted. Additionally, as fermentation progresses, the accumulation of metabolites may inhibit microorganisms, affecting their ability to synthesize specific proteins.

[0093] As the proportion of sulfur-rich proteins decreases, the proportion of carbohydrates and lipids increases accordingly. This is because when the synthesis of sulfur-rich amino acids is limited, microorganisms will turn to other metabolic pathways to maintain growth and survival, such as making greater use of carbon sources to generate carbohydrates or store energy as lipids.

[0094] In stages 8-10, the proportion of sulfur-rich proteins decreased significantly, while the proportions of carbohydrates and lipids increased markedly. This change indicates system aging or an imbalance in the microbial community structure. This further demonstrates that the synthesis of biomass as a subsequent feedstock additive should be carried out during stages one through seven.

[0095] The amino acid content (mg / gVSS) at each stage is shown in Table 4.

[0096] Table 4: Distribution of amino acid content in sulfur-rich organic matter at each experimental stage in Example 1 (mg / gVSS).

[0097]

[0098] Analysis of experimental data shows that microbial protein biomass from stages 1 to 7 exhibits the best composition and functionality, making it an ideal raw material for foliar fertilizer additives. During these stages, the proportion of microbial protein remains stable, with sulfur-rich complex amino acids (cysteine, methionine, and taurine) also maintaining a stable proportion in the total amino acid profile, and the proportions across stages are well-coordinated. Furthermore, the amino acid profiles at this stage are complete and evenly distributed, which is beneficial for synergistic nutritional effects. In contrast, the content of microbial protein and sulfur-rich amino acids in biomass from stages 8 to 10 decreases significantly, while the proportions of carbohydrates and lipids increase, resulting in a marked decrease in functionality. Considering amino acid composition, functionality, and process stability, stages 1 to 7 of the fermentation process are recommended as the biomass collection window to ensure the high efficiency and consistency of the final product. This further demonstrates that the synthesis time for biomass as a raw material for subsequent foliar fertilizer additives should be selected from stages 1 to 7.

[0099] Example 2

[0100] The difference between Example 2 and Example 1 is that 10% (v / v) of a mixed HOB-SOB microbial community was added to the microbial protein reactor. The microbial communities were the same, but their relative abundance differed. Specific relative abundance values ​​are shown in Table 5.

[0101] Table 5: Distribution of species and relative abundance of HOB, SOB and other microbial species in the microbial protein reactor in Example 2.

[0102]

[0103] The obtained data are shown in Tables 6, 7 and 8.

[0104] Table 6: Biomass yield (mgVSS / L) at each experimental stage in the microbial protein reactor in Example 2.

[0105]

[0106] Table 7: Content distribution (%) of major components in sulfur-rich organic matter at each experimental stage in Example 2.

[0107]

[0108] Table 8: Distribution of amino acid content in sulfur-rich organic matter at each experimental stage in Example 2 (mg / gVSS).

[0109]

[0110] Even if the intergeneric abundance within HOB and SOB is changed, the total abundance of HOB and SOB still meets the requirements, enabling efficient synthesis of microbial proteins. This ensures a stable proportion of sulfur-rich complex amino acids, meeting the functional amino acid composition requirements of this invention, and exhibiting versatility and stability.

[0111] Comparative Example 1

[0112] The difference between Comparative Example 1 and Example 1 is that the bacterial communities are the same, but the relative abundance of HOB and SOB varies significantly, while the relative abundance of other species is within the required range. Specific relative abundances are shown in Table 9.

[0113] Table 9: Distribution of species and relative abundance of HOB, SOB and other microbial species in the microbial protein reactor of Comparative Example 1.

[0114]

[0115] See Tables 10, 11 and 12 for data details.

[0116] Table 10: Biomass yield (mgVSS / L) at each experimental stage in the microbial protein reactor of Comparative Example 1.

[0117]

[0118] Table 11: Content distribution (%) of major components in sulfur-rich organic matter at each experimental stage in Comparative Example 1.

[0119]

[0120] Table 12: Distribution of amino acid content in sulfur-rich organic matter at each experimental stage in Comparative Example 1 (mg / gVSS).

[0121]

[0122] The main difference between Comparative Example 1 and Example 1 lies in the imbalance of the relative abundance of the microbial community, specifically, an excessively high proportion of HOB (hydrogen-oxidizing bacteria) and a low proportion of SOB (sulfur-oxidizing bacteria), disrupting the original balance. This change severely inhibited the synthesis of sulfur-containing amino acids such as cysteine, methionine, and taurine. As fermentation progressed, the proportion of sulfur-rich proteins continued to decrease, and the content of key amino acids also significantly declined, resulting in a substantial reduction in the overall nutritional value of the biomass. The results indicate that once the microbial community ratio deviates from the protection range, it directly causes a series of chain reactions, including the obstruction of functional metabolic pathways, the accumulation of byproducts, and a decrease in system stability, ultimately leading to a decrease in the synthesis efficiency of the target product and a deterioration in the effectiveness of the prepared foliar fertilizer additive. Therefore, maintaining a reasonable ratio between HOB and SOB is a crucial prerequisite for ensuring the efficient synthesis of sulfur-rich organic matter.

[0123] Comparative Example 2

[0124] The difference between Comparative Example 1 and Example 1 is that the bacterial communities are the same, but the relative abundance of HOB, SOB, and other bacterial communities is different. The specific relative abundances are shown in Table 13.

[0125] Table 13: Distribution of species and relative abundance of HOB, SOB and other microbial species in the microbial protein reactor of Comparative Example 2.

[0126]

[0127] See Tables 14, 15 and 16 for data details.

[0128] Table 14: Biomass yield (mgVSS / L) at each experimental stage in the microbial protein reactor in Comparative Example 2.

[0129]

[0130] Table 15: Content distribution (%) of major components in sulfur-rich organic matter at each experimental stage in Comparative Example 2.

[0131]

[0132] Table 16: Distribution of amino acid content in sulfur-rich organic matter at each experimental stage in Comparative Example 2 (mg / gVSS).

[0133]

[0134] In Comparative Example 2, when the abundance of other bacterial genera was relatively high, the complexity of the microbial ecosystem increased, competition among different functional species intensified, leading to decreased system stability, manifested as a generally low and fluctuating biomass yield. The proportion of sulfur-rich proteins was unstable at each stage, while the proportions of carbohydrates and lipids increased, reflecting a shift in metabolic pathways. An excessive proportion of other bacterial species disrupted the original ecological balance, which was detrimental to the stable generation of high-protein, sulfur-rich microbial biomass.

[0135] Example 3

[0136] The sulfur-rich organic matter from Example 1 was used to prepare a foliar fertilizer. The specific steps included:

[0137] Step S1: Prepare a 10% aqueous solution of 2 parts choline chloride and a 5% ethanol solution of 2 parts brassinolide. Mix the choline chloride solution and the brassinolide solution evenly.

[0138] Step S2

[0139] Mix 20 parts of sulfur-rich organic matter, 5 parts of tea polyphenols, 15 parts of bentonite and 4 parts of seaweed extract, and pulverize with an air jet mill for 15 min to make the particle size of the mixed powder about 180 mesh.

[0140] Step S3

[0141] Add 30 parts of deionized water and 5 parts of ethanol to the above-mentioned pulverized mixture, then transfer it to a high-speed homogenizer and shear and stir at 5000 rpm for 5 minutes to fully wet and initially disperse the components.

[0142] Step S4

[0143] The mixed solution of choline chloride and brassinolide prepared in step S1 is slowly added to the dispersion system in step S3. The stirring speed is maintained at 300 rpm and the stirring is continued for 5 minutes to ensure that the growth regulator is evenly distributed in the system. Then the mixed system is allowed to stand for 2 hours to allow the active ingredients to fully bind to the sulfur-rich organic carrier.

[0144] Step S5

[0145] Add 6 parts of polyvinyl alcohol and 4 parts of polymalic acid to the suspension in step S4, and continue stirring at 300 rpm for 5 minutes to ensure uniform dispersion of the slow-release agent; then add 0.5 parts of 10% hydroxypropyl methylcellulose aqueous solution to prevent particle sedimentation and enhance system stability.

[0146] Step S6

[0147] According to the system's flowability requirements, add an appropriate amount of deionized water or 10% hydroxypropyl methylcellulose aqueous solution to adjust the viscosity to between 50 mPa·s, and at the same time adjust the pH value of the system to between 6.5; after filtering the final uniform suspension through a filter screen to remove impurities, fill it into a light-proof container and seal it for storage.

[0148] Example 4

[0149] Sulfur-rich organic matter was selected from Example 2 for the preparation of foliar fertilizer.

[0150] Includes the following steps:

[0151] Step S1: Prepare a 10% aqueous solution of 4 parts choline chloride and a 5% ethanol solution of 3 parts brassinolide. Mix the choline chloride solution and the brassinolide solution evenly.

[0152] Step S2

[0153] Mix 30 parts of sulfur-rich organic matter, 10 parts of tea polyphenols, 18 parts of bentonite and 8 parts of seaweed extract, and pulverize in an air jet mill for 20 minutes to make the particle size of the mixed powder 180 mesh.

[0154] Step S3

[0155] Add 50 parts of deionized water and 10 parts of ethanol to the above-mentioned pulverized mixture, then transfer it to a high-speed homogenizer and shear and stir at 7000 rpm for 10 minutes to fully wet and initially disperse the components.

[0156] Step S4

[0157] The mixed solution of choline chloride and brassinolide prepared in step S1 is slowly added to the dispersion system in step S3. The stirring speed is kept at 500 rpm and stirred for 10 minutes to ensure that the growth regulator is evenly distributed in the system. Then the mixed system is allowed to stand for 2 hours to allow the active ingredients to fully bind to the sulfur-rich organic carrier.

[0158] Step S5

[0159] Add 6–8 parts of polyvinyl alcohol and 4–6 parts of polymalic acid to the suspension in step S4, and continue stirring at 500 rpm for 10 minutes to ensure uniform dispersion of the slow-release agent; then add 1 part of 10% hydroxypropyl methylcellulose aqueous solution to prevent particle sedimentation and enhance system stability.

[0160] Step S6

[0161] According to the system's flowability requirements, add an appropriate amount of deionized water or 10% hydroxypropyl methylcellulose aqueous solution to adjust the viscosity to between 60 mPa·s, and at the same time adjust the pH value of the system to between 6.5; after filtering the final uniform suspension through a filter screen to remove impurities, fill it into a light-proof container and seal it for storage.

[0162] Comparative Example 3

[0163] This comparative example is the same as Example 3, except that the amount of sulfur-rich organic matter added is 35 parts.

[0164] Example 5

[0165] The experiment was conducted in a greenhouse on an organic farm. The soil was alluvial soil, and the physicochemical properties of the topsoil were as follows: organic matter 35.62 g / kg, total nitrogen 0.49 g / kg, available phosphorus 15.6 mg / kg, water content 15.23%, Ec 384.52 μs / cm, and pH 7.51.

[0166] In this embodiment, control group 1 was treated with only conventional foliar fertilizer, while experimental group 1 was treated with the additive prepared in Example 3 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same. Experimental group 2 was treated with the additive prepared in Example 4 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same. Experimental group 3 was treated with the additive prepared in Comparative Example 3 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same.

[0167] During the peak fruiting period of strawberries, 10 strawberry fruits of uniform maturity were selected from each treatment for fruit quality determination. The results of amino acid determination are shown in Table 17.

[0168] Table 17: Results of amino acid content determination in strawberry fruits of control and experimental groups (g / 100g).

[0169]

[0170] As shown in the table above, the content of various amino acids in strawberry fruits increased after using foliar fertilizer additives containing specific amino acids. This indicates that foliar fertilizer additives have a positive effect on increasing the amino acid content in strawberry fruits. It demonstrates that plant leaves can absorb amino acids from sprayed fertilizers and transport them to other parts, including the fruit. Cysteine ​​and methionine are sulfur-containing amino acids, which are not only basic building blocks of protein synthesis but also play an important role in the antioxidant defense system. The sulfur-rich amino acids in foliar fertilizers can provide strawberries with more precursor substances, promoting the synthesis of amino acids within the plant. The unsatisfactory data in experimental group 3 may be due to the excessive sulfur-rich organic matter disrupting the interactions between components in the original formula system, interfering with the normal nutrient absorption and synthesis pathways within the plant.

[0171] The effects of soil parameters on strawberry growth are shown in Table 18.

[0172] Table 18: Comparison of soil physicochemical properties between the control group and the experimental group for strawberry growth.

[0173]

[0174] As shown in the table above, a small amount of fertilizer falls into the soil during the spraying process, thus altering the soil properties. The soil's organic matter, available phosphorus, and total nitrogen content increased, electrical conductivity rose slightly, pH remained stable, and water content increased, indicating that the additives improved soil fertility and water retention capacity to some extent.

[0175] The additives contain sulfur-rich organic matter, tea polyphenols, and growth regulators, providing abundant nutrients and promoting the effective conversion and release of nutrients in the soil. In particular, the presence of sulfur-containing amino acids and microbial active substances enhances the supply of essential nutrients such as phosphorus and nitrogen in the soil. The addition of bentonite and seaweed extract improves the soil's physical structure, increases its permeability and water retention capacity, and is beneficial for root growth and water management. The slow-release system composed of polyvinyl alcohol and polymalic acid ensures the slow release of nutrients, reduces nutrient loss, and improves fertilizer utilization.

[0176] The types of vitamins in the fruit were determined, and the results are shown in Table 19.

[0177] Table 19: Results of vitamin content determination in strawberry fruits of control and experimental groups (mg / 100g).

[0178]

[0179] Sulfur-rich organic matter, as a core functional component, is rich in sulfur-containing amino acids such as cysteine, methionine, and taurine. These substances are key precursors for the synthesis of various antioxidant metabolites in plants, including glutathione and vitamin C precursors. Cysteine ​​is one of the most critical sulfur-containing amino acids in plants, directly participating in the synthesis of glutathione (GSH). GSH is not only an important component of the plant's endogenous antioxidant system but also regulates the redox state of ascorbic acid (vitamin C), promoting its accumulation in cells. Choline chloride and brassinolide, growth regulators, can enhance photosynthetic efficiency and nutrient transport capacity by regulating plant hormone balance, thereby increasing the supply of carbohydrates and precursor substances, providing sufficient carbon skeletons and energy support for vitamin biosynthesis. Brassinolide is beneficial for the synthesis and accumulation of antioxidants such as vitamins. Seaweed extracts are rich in natural plant hormones and trace active substances, which can activate secondary metabolic pathways in plants, especially the vitamin E synthesis pathway. This additive promotes the accumulation of vitamins in fruits and vegetables on multiple levels by providing key precursors needed for vitamin synthesis, enhancing the plant's antioxidant defense system, and optimizing metabolic regulatory pathways, thus significantly improving their nutritional value and quality. Therefore, the application of the additive significantly increased the vitamin C and E content in strawberry fruits.

[0180] Example 6

[0181] The soil in the experimental field had a pH of 6.3, an organic matter content of 20.8 g / kg, a total nitrogen content of 1.2 g / kg, an available phosphorus content of 48.4 mg / kg, and a available potassium content of 360.0 mg / kg.

[0182] In this embodiment, control group 2 was treated with only conventional foliar fertilizer, while experimental group 4 was treated with the additive prepared in Example 3 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same. Experimental group 5 was treated with the additive prepared in Example 4 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same. Experimental group 6 was treated with the additive prepared in Comparative Example 3 at 10% of the total mass of diluted foliar fertilizer, which was then thoroughly mixed with conventional foliar fertilizer and applied together. All other experimental conditions were the same.

[0183] Ten cabbages of uniform maturity were selected from each treatment at the cabbage maturity stage for quality determination. The results of amino acid determination are shown in Table 20.

[0184] Table 20: Results of amino acid content determination in cabbage of control and experimental groups (g / kg).

[0185]

[0186] As can be seen from the table above, the content of various amino acids in Chinese cabbage increased after using foliar fertilizer additives containing specific amino acids.

[0187] The types of vitamins in Chinese cabbage were determined, and the results are shown in Table 21.

[0188] Table 21: Results of vitamin content determination in cabbage of the control group and the experimental group.

[0189]

[0190] It can be seen that the vitamin content in Chinese cabbage increased after using a foliar fertilizer additive containing specific amino acids. This foliar fertilizer additive contains various functional components such as sulfur-rich proteins and tea polyphenols, which can directly or indirectly provide plants with essential nutrients and promote their healthy growth. Sulfur-rich organic matter, as a core functional component, is rich in sulfur-containing amino acids such as cysteine, methionine, and taurine. These substances are key precursors for the synthesis of various antioxidant metabolites in plants (including glutathione and vitamin C precursors). For example, cysteine ​​is one of the most critical sulfur-containing amino acids in plants, directly participating in the synthesis of glutathione (GSH). GSH is not only an important component of the plant's endogenous antioxidant system but also regulates the redox state of ascorbic acid (vitamin C), promoting its accumulation in cells. Tea polyphenols have significant antioxidant properties, scavenging free radicals in plants and reducing the damage of oxidative stress to plant cells. This helps protect the stability of the plant's internal environment, thus promoting vitamin synthesis and accumulation. Plant growth regulators such as choline chloride and brassinolide can regulate plant growth and development processes, including promoting root growth, enhancing photosynthetic efficiency, and improving nutrient absorption. These factors work together to enable plants to utilize nutrients more effectively for metabolic activities, thereby promoting vitamin biosynthesis. Due to the combined effects of these factors, cabbage treated with this specific foliar fertilizer additive showed higher levels of vitamin K and vitamin C at maturity compared to the control group. This indicates that the foliar fertilizer additive used does indeed help improve the nutritional value of the crop.

[0191] The embodiments of this application have now been described in detail. To avoid obscuring the concept of this application, some details known in the art have not been described. Those skilled in the art can fully understand how to implement the technical solutions disclosed herein based on the above description.

[0192] While specific embodiments of this application have been described in detail by way of examples, those skilled in the art should understand that the above examples are for illustrative purposes only and are not intended to limit the scope of this application. Those skilled in the art should understand that modifications can be made to the above embodiments or equivalent substitutions can be made to some technical features without departing from the scope and spirit of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the embodiments can be combined in any manner.

Claims

1. A foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables, characterized in that, The raw materials include the following parts by weight: sulfur-rich organic matter: 20-30 parts, tea polyphenols: 5-10 parts, growth regulators: 4-7 parts, slow-release agents: 10-14 parts, bentonite: 15-18 parts, and seaweed extract: 4-8 parts; the sulfur-rich organic matter includes sulfur-rich proteins, which include sulfur-rich amino acids and other amino acids, with a weight percentage of 35%-60%: 40-65% for sulfur-rich amino acids and other amino acids; the sulfur-rich amino acids include cysteine, methionine, and taurine; among the sulfur-rich amino acids, the weight percentage of cysteine, methionine, and taurine is 30%-40%: 40%-45%: 15%-30%; The growth regulators include choline chloride and brassinolide, with choline chloride at 2-4 parts and brassinolide at 2-3 parts; the slow-release agents include polyvinyl alcohol and polymalic acid, with polyvinyl alcohol at 6-8 parts and polymalic acid at 4-6 parts. The preparation process is as follows: Step 1, use 10 m 3 The microbial protein reactor was maintained at a temperature of 30 °C; mineral salt culture medium was continuously supplied via a peristaltic pump, and the hydraulic retention time was set to 2.5 days. A mixture of gases with volume percentages of 15% CO2, 25% O2, 59% H2 and 1% H2S is introduced into the reactor, with a total gas flow rate of 8000 L / d. The gas is thoroughly mixed with the culture medium by an air pump and then injected into the microbial protein reactor through a nozzle. The microbial protein reactor is a spray type. Step 2: Add HOB-SOB mixed microbial community and other auxiliary microbial species to the microbial protein reactor to form a complex microbial system. The amount of the complex microbial system added is 10% of the volume of the microbial protein reactor. Step 3: Collect the bacterial cells every 14 days during the cultivation process, for a total of 7 times. Centrifuge the collected bacterial cells each time and freeze-dry them to obtain protein-rich biomass products. The complex microbial system includes HOBs, SOBs, and other auxiliary bacteria genera, with HOBs being... Hydrogenophaga Xanthobacter and Plesiomonas SOB is Thiobacillus,Acidithiobacillus,Sulfurovum and Halothiobacillus Other auxiliary bacteria genera are Nocardia,Burkholdefia,Sulfuriferula and Thioalkalivibrio ; The relative abundance of HOB was 30-40%, the relative abundance of SOB was 40-50%, and the relative abundance of other helper bacteria was 10-15%. The prepared foliar fertilizer improved soil fertility and water retention capacity.

2. The foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, The remaining amino acids include histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tyrosine, valine, alanine, arginine, aspartic acid, glycine, glutamic acid, and serine.

3. The foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, Sulfur-rich organic matter also includes carbohydrates, lipids and other substances. The weight ratio of sulfur-rich protein, carbohydrates, lipids and other substances is 65~80%: 5~10%: 10~20%: 5~10%.

4. The foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables according to claim 1, characterized in that, The pH of the culture medium was maintained at 7.0 using 0.2M phosphate buffer solution. The mineral salt culture medium formulation consisted of the following components: 0.7-1.0 parts of MgCl2·6H2O, 1.0-1.3 parts of NH4Cl, 1.1-1.5 parts of KH2PO4, 1.6-1.8 parts of Na2HPO4, and 0.1-0.5 parts of CaCl2·2H2O.

5. A method for preparing a foliar fertilizer additive containing sulfur-rich organic matter to enhance the nutritional value of fruits and vegetables, based on claim 1, characterized in that, Includes the following steps: Step S1: Prepare a 10% aqueous solution of 2-4 parts choline chloride and a 5% ethanol solution of 2-3 parts brassinolide. Mix the choline chloride solution and the brassinolide solution evenly. Step S2 Mix 20–30 parts of sulfur-rich organic matter, 5–10 parts of tea polyphenols, 15–18 parts of bentonite and 4–8 parts of seaweed extract, and pulverize with an air jet mill for 15–20 minutes to make the particle size of the mixed powder less than 200 mesh. Step S3 Add 30-50 parts of deionized water and 5-10 parts of ethanol to the above-mentioned pulverized mixture, then transfer it to a high-speed homogenizer and shear and stir at 5000-7000 rpm for 5-10 minutes to fully wet and initially disperse the components. Step S4 Slowly add the mixed solution of choline chloride and brassinolide prepared in step S1 to the dispersion system in step S3, and keep the stirring speed at 300-500 rpm for 5-10 minutes to make the growth regulator evenly distributed in the system; then let the mixed system stand for 2 hours to allow the active ingredients to fully bind to the sulfur-rich organic carrier. Step S5 Add 6–8 parts of polyvinyl alcohol and 4–6 parts of polymalic acid to the suspension in step S4, and continue stirring at 300–500 rpm for 5–10 minutes to ensure uniform dispersion of the slow-release agent; then add 0.5–1 parts of 10% hydroxypropyl methylcellulose aqueous solution to prevent particle sedimentation and enhance system stability. Step S6 According to the system's flowability requirements, add an appropriate amount of deionized water or 10% hydroxypropyl methylcellulose aqueous solution to adjust the viscosity to between 40-80 mPa·s, and at the same time adjust the pH value of the system to between 6 and 7; after filtering the final uniform suspension through a filter screen to remove impurities, fill it into a light-proof container and seal it for storage.

6. The application of the foliar fertilizer additive containing sulfur-rich organic matter as described in any one of claims 1-4 for enhancing the nutritional value of fruits and vegetables in foliar fertilizers.

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