A large granule dog and cat oral health functional food coated with instant freeze-dried granules and a preparation method thereof

Abstract

This application relates to a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules and its preparation method, belonging to the field of pet food technology. This formulation contains grain-based granules, an external spraying system consisting of 0.1-0.15 parts lecithin, 0.002-0.003 parts monoglycerides, 0.001-0.003 parts vitamin E, and 0.8-1.2 parts chicken fat, and an instant-dissolving freeze-dried microparticle layer consisting of 0.1-0.2 parts brown algae polysaccharide, 0.02-0.05 parts lysozyme, 0.04-0.08 parts tea polyphenols, 0.02-0.04 parts zinc gluconate, 0.05-0.1 parts gum arabic, 0.03-0.1 parts glutamine, 0.01-0.02 parts vitamin B2, 0.01-0.04 parts vitamin B12, and 0.02-0.07 parts glycine. Combined with large particles, it can stimulate saliva secretion, effectively inhibit the formation of dental plaque and tartar, and eliminate bad breath.

Description

Technical Field

[0001] This application relates to a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules and its preparation method, belonging to the field of pet food technology. Background Technology

[0002] Oral health problems are common in dogs and cats, primarily caused by the accumulation of plaque (a biofilm containing bacteria) on the tooth surface, which then mineralizes to form tartar, leading to gingivitis, halitosis, and even tooth loss. The root cause is that commercially available dog and cat food, produced through industrial design, is generally small and crumbly. Dogs and cats are not adept at chewing, so they swallow it directly without much biting, resulting in insufficient saliva production. Reduced saliva production leads to decreased oral self-cleaning ability, weakened pH regulation, and reduced antibacterial and bacteriostatic effects (saliva contains lysozyme, lactoperoxidase, etc.), preventing saliva from fully utilizing its natural defense functions.

[0003] In the prior art, the following solutions are commonly used to address this problem: 1) Mechanically cleanable food, which removes plaque by rubbing teeth with fibers or special structures during chewing. For example, US patent application US5431927A describes a pet food with an expanded stripe structure that breaks during chewing to provide a mechanical cleaning effect. However, the cleaning effect of such products is limited to the molars or tooth tips, lacking a comprehensive mechanism of action for the entire oral cavity; 2) Food with added oral care agents, which directly mixes antibacterial and anti-tartar ingredients into the feed. For example, US patent application US2017 / 0339978A1 improves the tooth cleaning effect by covalently binding hydrolyzed protein with high-branched starch. However, most active ingredients are prone to denaturation during high-temperature expansion, and the internal mixing method makes it difficult to ensure the effective release of ingredients in the oral cavity, failing to form an effective concentration locally; 3) Locally released products, such as sprays and gels, require manual operation, have poor compliance with dogs and cats, and since oral problems are closely related to intestinal microecological imbalance, these products are limited to local oral treatment and lack systemic regulation.

[0004] In response to the problems of small pellet size, short chewing time, insufficient saliva secretion, and easy deactivation of active ingredients during processing and difficulty in effective release in the oral cavity in existing pet food technologies, the market urgently needs a food solution that can provide effective mechanical cleaning, fully stimulate saliva secretion, and accurately release active ingredients into the oral cavity. Summary of the Invention

[0005] To address the aforementioned issues, a large-particle functional oral health food for dogs and cats, coated with quick-dissolving freeze-dried granules, and its preparation method are provided. The large-particle design, combined with chewing, effectively stimulates saliva secretion. The coating technology attaches quick-dissolving freeze-dried active ingredients to the surface of the large particles, effectively inhibiting plaque and tartar formation, eliminating bad breath, stopping bleeding, and relieving gingivitis. Furthermore, through synergistic regulation of the oral-intestinal microecology, it can significantly improve the overall health of dogs and cats. Compared to existing technologies, it has the advantages of comprehensive oral coverage, good release of active ingredients, and comprehensive regulation of the oral-intestinal system, giving it strong market competitiveness.

[0006] This application provides a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules, comprising:

[0007] a) The internal food-based pellets, wherein when used for dogs, the pellets have a size ≥20mm and a hardness of 15~25kgf / cm. 2 When used for cat food, the particle size should be ≥12mm and the hardness should be 12~20kgf / cm. 2 ;

[0008] b) An external spraying system on the surface of the grain base particles, the external spraying system comprising the following components in parts by weight: 0.1-0.15 parts lecithin, 0.002-0.003 parts monoglycerides, 0.001-0.003 parts vitamin E and 0.8-1.2 parts chicken fat;

[0009] c) The outermost layer of quick-dissolving freeze-dried microparticles, wherein the quick-dissolving freeze-dried microparticles comprises the following components in parts by weight: 0.1-0.2 parts of brown algae polysaccharide, 0.02-0.05 parts of lysozyme, 0.04-0.08 parts of tea polyphenols, 0.02-0.04 parts of zinc gluconate, 0.05-0.1 parts of gum arabic, 0.03-0.1 parts of glutamine, 0.01-0.02 parts of vitamin B2, 0.01-0.04 parts of vitamin B12, and 0.02-0.07 parts of glycine.

[0010] Optionally, the total weight of the quick-dissolving freeze-dried microparticle layer is 0.3% to 0.7% of the total weight of the grain base particles;

[0011] Optionally, the total weight of the quick-dissolving freeze-dried microparticle layer is 0.35 to 0.6% of the total weight of the grain base particles.

[0012] Optionally, the total weight of the external spraying system is 0.5% to 2.5% of the total weight of the grain base particles;

[0013] Optionally, the total weight of the external spraying system is 0.8 to 1.5% of the total weight of the grain base particles.

[0014] Optionally, the mesh size of the rapidly lyophilized microparticles in the rapidly lyophilized microparticle layer is not higher than 80.

[0015] Optionally, the mesh size of the quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer is not higher than 60.

[0016] Optionally, the external spraying system further includes 0.1-0.3 parts of octenyl succinate starch ester and 0.02-0.08 parts of chitosan oligosaccharide;

[0017] Optionally, the external spraying system further includes 0.15-0.25 parts of octenyl succinate starch ester and 0.03-0.06 parts of chitosan oligosaccharide.

[0018] Optionally, the rapidly soluble lyophilized microparticle layer further includes 0.05-0.2 parts pullulan and 0.05-0.2 parts β-cyclodextrin;

[0019] Optionally, the quick-dissolving lyophilized microparticle layer may further include 0.08-0.15 parts pullulan and 0.08-0.15 parts β-cyclodextrin.

[0020] This application provides a method for preparing the above-mentioned large-particle canine and feline oral health functional food with an outer coating of quick-dissolving freeze-dried granules, the preparation method comprising the following steps:

[0021] Prepare grain-based feed pellets;

[0022] The components of the quick-dissolving freeze-dried microparticle layer are dissolved and mixed in water, and then freeze-dried to produce quick-dissolving freeze-dried microparticles.

[0023] Prepare an external spraying system and spray it onto the surface of grain base particles;

[0024] The product is obtained by coating the surface of grain base particles that have been sprayed with an external coating system with quick-dissolving freeze-dried microparticles.

[0025] Optionally, before coating the quick-dissolving freeze-dried microparticles, a step of moisture control treatment is included for the grain base particles coated with the external spraying system. The conditions for moisture control treatment include: treatment for 3 to 10 minutes in an environment with a relative humidity of 75 to 90% and a temperature of 20 to 30°C.

[0026] Optionally, the step of coating the surface of the grain base particles coated with the external spraying system with the quick-dissolving freeze-dried microparticles specifically includes: under a temperature condition of ≤20℃, using a pulsed airflow with an airflow speed of 5~25 m / s and 0.5~10 Hz to directionally apply the quick-dissolving freeze-dried microparticles to the surface of the grain base particles coated with the external spraying system, and processing for 10~60s to allow the quick-dissolving freeze-dried microparticles to be embedded in the outermost layer.

[0027] Optionally, the step of preparing the external spraying system and externally spraying it onto the surface of the grain base particles specifically includes: mixing the raw materials of the external spraying system and then uniformly coating them onto the surface of the grain base particles preheated to 35~45℃ using a spraying device.

[0028] Optionally, the step of mixing the components of the quick-dissolving freeze-dried microparticle layer and freeze-drying it to obtain quick-dissolving freeze-dried microparticles specifically includes: mixing the components of the quick-dissolving freeze-dried microparticle layer and then homogenizing them, followed by pre-freezing, and finally freeze-drying to obtain quick-dissolving freeze-dried microparticles.

[0029] Optionally, the homogenization conditions include: a rotation speed of 3000~8000 rpm and a time of 2~5 min.

[0030] Optionally, the pre-freezing treatment includes the steps of first treating at -10~-20℃ for 30~60 min, and then treating at -35~-45℃ for 2~4 h.

[0031] Optionally, the freeze-drying process includes primary drying and secondary drying, wherein primary drying is carried out at -30 to -20°C and a vacuum of 10 to 50 Pa for 12 to 24 hours, and secondary drying is carried out at 10 to 25°C for 4 to 8 hours.

[0032] Optionally, the moisture content of the material obtained after freeze-drying is not higher than 5%.

[0033] Optionally, the freeze-drying process further includes the steps of crushing and sieving the resulting material;

[0034] Optionally, sieve to obtain quick-dissolving freeze-dried microparticles with a particle size not exceeding 80 mesh.

[0035] Optionally, sieving yields quick-dissolving freeze-dried microparticles with a particle size not exceeding 60 mesh.

[0036] Optionally, the crushing and screening steps are carried out at a temperature not exceeding 25°C.

[0037] Optionally, the grain-based feed pellets include chicken meal, fish meal, pea protein, brown rice, fish oil, and vitamin and mineral premix.

[0038] The beneficial effects of this application include, but are not limited to:

[0039] 1. The large-particle canine and feline oral health functional food with an outer coating of quick-dissolving freeze-dried particles and its preparation method, based on the physical design of the large-particle food, can promote pet chewing and thus stimulate saliva secretion. The secreted saliva can act on the quick-dissolving freeze-dried microparticles in the outermost layer of quick-dissolving freeze-dried microparticles. The quick-dissolving freeze-dried microparticles are combined with the quick-dissolving freeze-dried active ingredients of the outermost layer through an external spraying system. This design scheme can achieve a high release rate of effective active ingredients during pet feeding based on the physiological needs of dogs and cats to chew food normally and to stimulate effective saliva secretion in this process. The quick-dissolving freeze-dried microparticles can be released instantly upon contact with saliva when the pet eats, covering the entire oral environment and producing a highly efficient synergistic effect with saliva components, thereby inhibiting dental plaque, reducing tartar, eliminating bad breath, and relieving gingivitis. In addition, the effective active ingredients can further enter the intestine to exert a synergistic regulatory effect on the oral-intestinal microecology.

[0040] 2. The large-particle canine and cat oral health functional food with an outer coating of quick-dissolving freeze-dried granules and its preparation method, as described in this application, generally suffers from problems such as small pet food particles, short chewing time, insufficient saliva secretion, and easy inactivation of active ingredients during processing, or difficulty in effective and comprehensive release in the oral cavity, or insufficient release amount. The structural design of the present application ensures that the effective active ingredients can be stably fixed on the outermost layer of the food base particles, with very little loss under collision or friction, and can be quickly released after encountering saliva in the oral cavity, fully covering the entire oral cavity with good release effect. Compared with the existing technical solutions, which work synergistically with saliva, it shows obvious technical advantages and has good market competitiveness.

[0041] 3. The large-particle canine and cat oral health functional food with outer coating of quick-dissolving freeze-dried particles and its preparation method, based on the effective active ingredients in the quick-dissolving freeze-dried microparticle layer, adds pullulan polysaccharide and β-cyclodextrin, which not only facilitates the preparation and shaping of freeze-dried particles, but also allows them to interact and adhere better with the components in the external spraying system, thereby improving the fixation effect of quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer on the grain base particles, and significantly reducing the amount of particles falling off under conditions such as collision or friction.

[0042] 4. According to the large-particle canine and cat oral health functional food with quick-dissolving freeze-dried granules coated by this application and its preparation method, the grain base particles are preheated during the external spraying system to ensure the adhesion and smoothness of the external spraying system. Furthermore, the preparation process also includes a humidity control process and a treatment method that uses pulsed airflow to directionally apply quick-dissolving freeze-dried microparticles to the surface of the grain base particles, which can significantly improve the fixation effect of quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer. Detailed Implementation

[0043] The present application is described in detail below with reference to the embodiments, but the present application is not limited to these embodiments. Unless otherwise specified, the raw materials and reagents in the embodiments of the present application are all purchased through commercial channels.

[0044] The present application solution will be described below through specific embodiments.

[0045] Example 1

[0046] 1) Prepare grain base pellets

[0047] As a dog-grade kibble, it has a size of 25 mm and a hardness of 21 kgf / cm². 2 The grain-based feed pellets are prepared from chicken meal, fish meal, pea protein, brown rice, fish oil and vitamin and mineral premix. The preparation of the grain-based feed pellets is an existing technology, and other conventional dog and cat food pellet raw materials that meet the requirements of pellet size and hardness can also be used as substitutes.

[0048] 2) Prepare the quick-dissolving freeze-dried microparticle layer

[0049] It includes the following components in parts by weight: 0.15 parts of brown algae polysaccharide, 0.04 parts of lysozyme, 0.06 parts of tea polyphenols, 0.03 parts of zinc gluconate, 0.08 parts of gum arabic, 0.07 parts of glutamine, 0.015 parts of vitamin B2, 0.03 parts of vitamin B12, 0.05 parts of glycine, 0.12 parts of pullulan polysaccharide, and 0.12 parts of β-cyclodextrin.

[0050] The preparation of the fast-dissolving freeze-dried microparticles includes: adding brown algae polysaccharide, gum arabic and pullulan polysaccharide to water, stirring and dissolving at 50°C to form a homogeneous film-forming phase solution; then lowering the system temperature to no higher than 40°C, and sequentially adding lysozyme, tea polyphenols, β-cyclodextrin, zinc gluconate, glutamine, vitamin B2, vitamin B12 and glycine, stirring evenly to form a mixed solution. When adding β-cyclodextrin, it is necessary to add it slowly while maintaining stirring, and continue stirring for 3 minutes after the addition is completed.

[0051] The resulting mixture was homogenized under the following conditions: a rotation speed of 6000 rpm for 2 minutes to improve the homogeneity of the system. After homogenization, the mixture was pre-frozen by first treating it at -15°C for 45 minutes and then at -40°C for 3 hours to form a frozen system with a porous structure.

[0052] The material was then subjected to freeze-drying, which included primary and secondary drying. The primary drying was carried out at -25°C and a vacuum of 30 Pa for 18 hours, and the secondary drying was carried out at 15°C for 6 hours, so that the moisture content of the resulting material was not higher than 5%. After freeze-drying, the resulting material was crushed and sieved at a temperature not higher than 25°C to obtain fast-dissolving freeze-dried microparticles with a particle size not higher than 80 mesh.

[0053] 3) Add an external spraying system

[0054] It includes the following ingredients in parts by weight: 0.12 parts lecithin, 0.002 parts monoglyceride, 0.002 parts natural vitamin E, 0.2 parts octenyl succinate starch ester, 0.04 parts chitosan oligosaccharide, and 0.9 parts chicken fat;

[0055] Preparation and application of the external spray coating system: Chicken oil was heated to 60°C to make it flow. Lecithin and monoglyceride were added sequentially under stirring to fully disperse them and form an oil phase system. Octenyl succinate starch ester was then added and stirring was continued to form a stable interfacial dispersion structure. When the system temperature dropped below 40°C, chitosan oligosaccharide and natural vitamin E were added and stirring was continued for 5 min to obtain a uniform and stable external spray coating system.

[0056] The obtained external spraying system is evenly sprayed onto the surface of grain base particles preheated to 40°C using a spraying equipment. At the same time, the particles are turned or rolled to ensure that the external spraying system is evenly covered on the particle surface. The spraying amount is controlled within the following range: the total weight of the external spraying system is 1.5% of the total weight of the grain base particles. After spraying, the mixture is left to stand for 20 minutes to remove surface air bubbles and ensure that the coating is uniform and continuous.

[0057] 4) Final product particle preparation

[0058] The grain base particles after spraying are subjected to moisture control treatment. The moisture control treatment conditions include: treatment for 5 minutes in an environment with a relative humidity of 80% and a temperature of 25℃, so that the hydrophilic components in the external spraying system undergo interfacial migration, thereby forming a semi-wet layer with a certain degree of adhesion on the particle surface, so as to improve the adhesion of the quick-dissolving freeze-dried microparticles.

[0059] Subsequently, at a temperature not exceeding 20°C, a pulsed airflow with an airflow speed of 15 m / s and a pulse frequency of 3 Hz was used to directionally apply the quick-dissolving freeze-dried microparticles to the surface of the sprayed grain base particles. The treatment time was 40 s, during which the grain base particles were kept in a rolling or turning state, so that the quick-dissolving freeze-dried microparticles were embedded in the surface of the outer spraying system, forming the outermost quick-dissolving freeze-dried microparticle layer. The total weight of the quick-dissolving freeze-dried microparticle layer was 0.4% of the total weight of the grain base particles. After the outer coating treatment, the mixture was left to stand for 3 hours until it was completely cooled before packaging, thus obtaining the large-particle canine and cat oral health functional food coated with quick-dissolving freeze-dried microparticles as described in this application.

[0060] Example 2

[0061] This embodiment is basically the same as Embodiment 1, except that it is a cat-specific pellet with a size of 15mm and a hardness of 15kgf / cm. 2 .

[0062] Example 3

[0063] This embodiment is basically the same as Embodiment 1, except that the external spraying system does not include octenyl succinate starch ester and chitosan oligosaccharide.

[0064] Example 4

[0065] This embodiment is basically the same as Example 1, except that pullulan and β-cyclodextrin are not included in the quick-dissolving freeze-dried microparticle layer.

[0066] Example 5

[0067] This embodiment is basically the same as Embodiment 1, except that the mesh size of the quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer is not higher than 120.

[0068] Example 6

[0069] This embodiment is basically the same as Embodiment 1, except that it does not include a humidity control treatment step.

[0070] Example 7

[0071] This embodiment is basically the same as Embodiment 1, except that in the step of coating the grain base particles with the external spraying system, the quick-dissolving freeze-dried microparticles are directly mixed with the grain base particles coated with the external spraying system at room temperature. The quick-dissolving freeze-dried microparticles are then brought into contact with and adhered to the particle surface by slight turning and vibration. The processing time is 15 minutes, and there is no pulse airflow directional effect.

[0072] Example 8

[0073] This embodiment is basically the same as embodiment 1, except that when the raw materials of the external spraying system are mixed and uniformly coated on the surface of the grain base particles that are preheated to 35~45℃ by the spraying equipment, the grain base particles are at room temperature and have not been preheated to 40℃.

[0074] Comparative Example 1

[0075] This comparative example is basically the same as Example 1, except that it is also made of dog pellets but the size is 10 mm.

[0076] Comparative Example 2

[0077] This comparative example is basically the same as Example 1, except that it does not contain the quick-dissolving freeze-dried microparticle layer.

[0078] Comparative Example 3

[0079] This comparative example is basically the same as Example 1, except that the external spraying system is not sprayed.

[0080] Comparative Example 4

[0081] This comparative example is basically the same as Example 1, except that the quick-dissolving freeze-dried microparticle layer is not coated externally, but is mixed in the grain base particles that have been sprayed with an external coating system.

[0082] Test Example 1

[0083] Researchers conducted canine animal experiments on the large-particle canine and cat oral health functional food product with outer coating of quick-dissolving freeze-dried granules prepared in Example 1. The specific tests included the following.

[0084] In the in vitro antibacterial experiment, the bacterial species included Porphyromonas gingivalis, Streptococcus mutans, and Actinomycetes. The method used was the agar diffusion method, and the diameter of the inhibition zone was measured.

[0085] In the saliva secretion stimulation experiment, the cotton ball weighing method was used to measure the amount of saliva secreted within 5 minutes before and after feeding.

[0086] In the plaque inhibition test, the Logan & Boyce index method (0-5 points) was used for weekly evaluation.

[0087] In the halitosis improvement experiment, a volatile sulfur compound detector (VSC detection, unit ppb) was used in combination with sensory evaluation.

[0088] In the gut microbiota regulation experiment, 16S rRNA sequencing was used to analyze the relative abundance of Lactobacillus, Bifidobacterium, and Escherichia coli in feces.

[0089] In the palatability test, the two-basin method was used to record the pets' initial choice of different foods and their continued food intake.

[0090] The results are shown in Table 1 below.

[0091] Table 1. Test results of large grains in Example 1

[0092]

[0093] In Table 1, "-" indicates that the experiment was not conducted, so the result is empty. Untested items are not included in the comparative analysis of the corresponding indicators.

[0094] As shown in Table 1, the large-particle canine and cat oral health functional food with quick-dissolving freeze-dried granules provided in this application significantly improves oral health effects and demonstrates excellent antibacterial, saliva secretion promotion, plaque removal, halitosis reduction, and intestinal flora regulation functions in the experiment.

[0095] Researchers found that in Comparative Example 1, because the size of the canine pellets was 10 mm, the dogs chewed the pellets significantly less during the experiment, resulting in insufficient saliva secretion. As a result, the quick-dissolving freeze-dried microparticle layer was difficult to dissolve in the oral cavity and exert its effect, which was not effective in inhibiting the formation of dental plaque and tartar and eliminating bad breath.

[0096] In Comparative Example 2, the use of large-particle food did increase saliva production in the mouths of the experimental dogs; however, its effect on inhibiting plaque and tartar formation and eliminating halitosis was very limited.

[0097] Comparative Example 3 is the rejected solution during the product development process of the experimenters. It was found that the quick-dissolving freeze-dried microparticle layer of this solution could not be coated on the surface of the grain base particles. Even if some quick-dissolving freeze-dried microparticles were coated on the surface, they could easily detach after packaging, such as during transportation and movement, and the structural stability was too poor.

[0098] In Comparative Example 4, when the quick-dissolving freeze-dried microparticles were mixed into the grain base particles, the quick-dissolving freeze-dried microparticle layer, when combined with large grain particles for pets to chew, could not exert the excellent effect of dissolving and acting on the whole oral cavity, and was also not conducive to pets' intake to better improve the intestinal environment.

[0099] Experimental Example 1

[0100] Researchers conducted product performance tests on the large-particle food obtained in Examples 1 and 3-8, mainly testing the changes in dental plaque index and the effect on halitosis improvement in dogs, to measure the dissolution effect of fast-dissolving freeze-dried microparticles in the oral cavity under the action of saliva during the process of dogs chewing large-particle food.

[0101] In addition, to evaluate the stability of the instant freeze-dried microparticles fixed on the surface of the obtained food pellets, the product used for testing plaque index changes and halitosis improvement in dogs was shaken before each feeding to simulate the impact of packaging, transportation, and movement on the instant freeze-dried microparticles on the surface. Specifically, large-particle canine and cat oral health functional food with packaged instant freeze-dried microparticles was taken and continuously shaken for 60 minutes at room temperature on a mechanical vibration table with a shaking frequency of 2 Hz and a shaking amplitude of 50 mm before being fed to the experimental dogs.

[0102] The changes in dental plaque index and the improvement in halitosis in dogs are shown in Table 2 below.

[0103] Table 2 Test results of large grain particles in Examples 1, 3-8

[0104]

[0105] Table 2 (Continued)

[0106]

[0107] According to the results in Table 2, the proposed embodiment 1 of this application can significantly improve the fixation effect of the quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer by optimizing the external spraying system and the composition of the quick-dissolving freeze-dried microparticle layer, as well as optimizing the moisture control treatment and the quick-dissolving freeze-dried microparticle coating process. It can also ensure that the saliva produced by the dog during the chewing process is released well in the oral cavity, thereby effectively inhibiting the formation of dental plaque and tartar and eliminating bad breath.

[0108] Furthermore, during the research, the researchers found that compared to Examples 6 and 7, adding a humidity control treatment and using an improved pulsed airflow spraying method significantly improved the fixation effect of the quick-dissolving freeze-dried microparticles. Experiments showed that this greatly reduced the amount of quick-dissolving freeze-dried microparticles detached due to collisions and friction. Additionally, compared to Example 8, adding a preheating process to 40°C during spraying also significantly improved the uniformity of the external spraying system, reducing unevenness and thus improving the fixation effect of the quick-dissolving freeze-dried microparticles. In Example 5, because the mesh size of the quick-dissolving freeze-dried microparticles used was too high, the microparticles were more easily detached due to collisions and friction, affecting the effectiveness of allowing the quick-dissolving freeze-dried microparticle layer to be fully released under the action of saliva when pets ingest the food product.

[0109] Experimental Example 2

[0110] Before finalizing the proposed solution, the researchers conducted extensive exploration and experiments on the composition of the quick-dissolving freeze-dried microparticle layer and the external spraying system.

[0111] The external spraying system mainly serves as a carrier platform for the rapidly soluble freeze-dried microparticles in the rapidly soluble freeze-dried microparticle layer. It must not only ensure a good fixation effect on the rapidly soluble freeze-dried microparticle layer, but also not affect the good dissolution effect of the rapidly soluble freeze-dried microparticles under the action of saliva in the oral cavity.

[0112] The main active ingredients and their functions in the quick-dissolving freeze-dried microparticle layer are shown in Table 3 below.

[0113] Table 3. Main active ingredients and their functions in the rapidly soluble lyophilized microparticle layer

[0114]

[0115] During the experiment, it was found that if only the main active ingredients in the quick-dissolving freeze-dried microparticle layer in Table 3 above are used to prepare quick-dissolving freeze-dried microparticles, the effect of fixing the quick-dissolving freeze-dried microparticles on the surface of the external spraying system is very poor. The quick-dissolving freeze-dried microparticles on the surface are easily detached due to collision and friction, which is not conducive to the quick-dissolving freeze-dried microparticles dissolving under the action of saliva when the pet chews in its mouth, and thus covering the entire oral cavity.

[0116] To this end, the researchers conducted in-depth research and experiments to ensure that the interface between the quick-dissolving freeze-dried microparticle layer and the external spraying system meets certain stability requirements while ensuring that the release under the action of saliva is not affected. The following tables 4 and 5 list some of the experimental exploration contents and corresponding results to illustrate the technical advantages of the final solution of this application. Table 4 only lists the differences between Example 1 and other test samples; all others are the same as Example 1.

[0117] Table 4 Optimization of the rapid-dissolving freeze-dried microparticle layer and external spraying system

[0118]

[0119] The test conditions were the same as in Experiment 1, in which the dogs were fed after being shaken. The results are shown in Table 5 below.

[0120] Table 5. Changes in dental plaque index and improvement in halitosis in dogs.

[0121]

[0122] Table 5 (Continued)

[0123]

[0124] Based on the above results, it can be seen that in the proposed solution, the optimized fast-dissolving lyophilized microparticle layer component and the optimized external spraying system component have a good synergistic effect. This ensures that the fast-dissolving lyophilized microparticles in the fast-dissolving lyophilized microparticle layer are well fixed on the surface of the external spraying system, and does not affect the effect of the fast-dissolving lyophilized microparticles rapidly dissolving and covering the entire oral cavity under the action of saliva.

[0125] The schemes in test sample 1, test sample 2, test sample 3 and test sample 4 performed poorly in the collision and friction experiments, and will not be used or tested in the future.

[0126] In particular, for test sample 7, the researchers found that adding gelatin and pectin to both the external spraying system and the quick-dissolving freeze-dried microparticle layer significantly improved the fixation effect of the quick-dissolving freeze-dried microparticles on the surface, demonstrating a clear advantage in collision and friction tests. However, the added gelatin and pectin made it difficult for the quick-dissolving freeze-dried microparticles to dissolve quickly under the action of saliva secreted during pet chewing. As a result, the quick-dissolving freeze-dried microparticles mainly functioned in the digestive tract, such as the intestines, after the mouth. Their effectiveness in inhibiting plaque and tartar formation and eliminating bad breath in the mouth was reduced. This was also verified in the relative abundance test results of the intestinal flora of experimental dogs. Compared with the proposed solution, the gelatin + pectin solution showed a higher relative abundance of lactobacilli and bifidobacteria and a lower abundance of Escherichia coli in the intestines of experimental dogs, demonstrating the significant effect of the quick-dissolving freeze-dried microparticles in the intestines.

[0127] The above description is merely an embodiment of this application, and the scope of protection of this application is not limited to these specific embodiments, but is determined by the claims of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the technical concept and principles of this application should be included within the scope of protection of this application.

Claims

1. A method for preparing a large-particle canine and cat oral health functional food coated with quick-dissolving freeze-dried granules, characterized in that, include: a) The internal food-based pellets, wherein when used for dogs, the pellets have a size ≥20mm and a hardness of 15~25kgf / cm. 2 When used for cat food, the particle size should be ≥12mm and the hardness should be 12~20kgf / cm. 2 b) An external spraying system on the surface of the grain base particles, the external spraying system comprising the following components in parts by weight: lecithin 0.1-0.15 parts, monoglyceride 0.002-0.003 parts, vitamin E 0.001-0.003 parts, octenyl succinate starch ester 0.1-0.3 parts, chitosan oligosaccharide 0.02-0.08 parts, and chicken fat 0.8-1.2 parts; c) The outermost quick-dissolving freeze-dried microparticle layer, the quick-dissolving freeze-dried microparticle layer comprising the following components in parts by weight: alginic acid 0.1-0.2 parts, lysozyme 0.02-0.05 parts, tea polyphenols 0.04-0.08 parts, zinc gluconate 0.02-0.04 parts, gum arabic 0.05-0.1 parts, glutamine 0.03-0.1 parts, vitamin B2 0.01-0.02 parts, and vitamin B12. 0.01~0.04 parts, pullulan 0.05~0.2 parts, β-cyclodextrin 0.05~0.2 parts, and glycine 0.02~0.07 parts; The preparation method includes the following steps: preparing grain base particles; dissolving and mixing the components of the quick-dissolving freeze-dried microparticle layer in water, and then freeze-drying to produce quick-dissolving freeze-dried microparticles; Prepare an external spraying system and spray it onto the surface of grain base particles; The grain base material particles coated with the external spraying system are subjected to moisture control treatment. The conditions of the moisture control treatment include: treating in an environment with a relative humidity of 75~90% and a temperature of 20~30℃ for 3~10 minutes, and coating the surface of the grain base material particles coated with the external spraying system with quick-dissolving freeze-dried microparticles. Specifically, under the temperature condition of ≤20℃, the quick-dissolving freeze-dried microparticles are directed onto the surface of the grain base material particles coated with the external spraying system using a pulsed airflow with an airflow speed of 5~25 m / s and a frequency of 0.5~10 Hz, and the treatment time is 10~60 seconds, so that the quick-dissolving freeze-dried microparticles are embedded in the outermost layer.

2. The large-particle canine and cat oral health functional food with an outer coating of quick-dissolving freeze-dried granules as described in claim 1, characterized in that, The total weight of the quick-dissolving freeze-dried microparticle layer is 0.3 to 0.7% of the total weight of the grain base particles.

3. The large-particle dog and cat oral health functional food with an outer coating of quick-dissolving freeze-dried granules as described in claim 1, characterized in that, The total weight of the external spraying system is 0.5 to 2.5% of the total weight of the grain base particles.

4. The large-particle dog and cat oral health functional food with an outer coating of quick-dissolving freeze-dried granules as described in claim 1, characterized in that, The mesh size of the quick-dissolving freeze-dried microparticles in the quick-dissolving freeze-dried microparticle layer is no higher than 80.

5. The large-particle canine and cat oral health functional food with an outer coating of quick-dissolving freeze-dried granules as described in claim 1, characterized in that, The steps of preparing the external spraying system and externally spraying it onto the surface of grain base particles specifically include: mixing the raw materials of the external spraying system and then uniformly coating them onto the surface of grain base particles preheated to 35~45℃ using a spraying device.

Images