Oil-soluble camellia extract, its extraction method and application

Abstract

The present application relates to an oil-soluble camellia extract, its extraction method and application, and belongs to the technical field of natural extracts. The present application provides an extraction method of an oil-soluble camellia extract, which comprises mixing camellia with an oily solvent and heating to extract the oil-soluble camellia extract. The oil-soluble camellia extract extracted by using the oily solvent as an extraction reagent has high solubility in various oils and other oily reagents, can be mutually soluble at any ratio, and does not have the phenomena of stratification and precipitation, and has excellent solubility. The present application also provides an anti-wrinkle composition, which comprises the oil-soluble camellia extract, ginseng flower oil and saussurea involucrata essence oil. The present application finds through free radical scavenging experiments and cell experiments that the oil-soluble camellia extract, ginseng flower oil and saussurea involucrata essence oil have the synergistic effects of anti-oxidation and anti-wrinkle.

Description

Technical Field

[0001] This invention relates to the field of natural extract technology, and in particular to an oil-soluble camellia extract, its extraction method, and its application. Background Technology

[0002] In today's skincare industry, as consumers' demand for anti-wrinkle and skin rejuvenation continues to rise, highly effective anti-wrinkle products have become the focus of the market. Due to aging, long-term UV exposure, poor lifestyle habits, and environmental pollutants, skin is prone to collagen loss and elastin fiber breakage, leading to deepening wrinkles, sagging skin, and other aging problems.

[0003] Currently, mainstream anti-wrinkle ingredients on the market include peptides, retinol, and vitamin C. In addition, plant-derived ingredients also demonstrate unique advantages in anti-wrinkle effects, such as centella asiatica extract, ginseng extract, and ginkgo biloba extract.

[0004] However, the aforementioned mainstream anti-wrinkle ingredients (such as peptides and retinol) are generally water-soluble or require water-based carriers, facing challenges in solubility and dispersibility in pure oil systems (such as skincare oils and ointments). Due to the low polarity and lack of a water-molecule environment in the oil phase, water-soluble active ingredients are prone to precipitation, aggregation, or inactivation, resulting in low penetration efficiency and limited efficacy release in pure oil formulations. This leads to the poor performance of existing pure oil products in anti-wrinkle applications, failing to meet consumers' demands for highly effective anti-wrinkle and deep nourishing effects from oil-based formulations.

[0005] Furthermore, existing technologies have several limitations in the application of commercially available camellia extract, a potentially anti-wrinkle ingredient. Camellia extract performs poorly in oil-phase systems, such as facial oils and conditioners. Its inherent solubility makes it difficult to disperse effectively and stably in these oily products, directly impacting their anti-wrinkle effects.

[0006] Therefore, there is an urgent need to develop a composition that can be used in pure oil formulations and has anti-wrinkle effects. At the same time, it is necessary to combine it with camellia extraction methods to improve the solubility and stability of camellia extract in the oil phase system, thereby enhancing the anti-wrinkle efficacy of the product and the user experience, and expanding the application scope of camellia extract in the cosmetics industry. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of the prior art and provide an oil-soluble camellia extract that can be used in a variety of oil-phase systems, as well as its extraction method and application.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] In a first aspect, the present invention provides a method for extracting an oil-soluble camellia extract by mixing camellia flowers with an oily solvent and heating to extract the oil-soluble camellia extract.

[0010] The oil-soluble camellia extract obtained by this invention using an oily solvent as the extraction reagent has high solubility in various oily reagents such as oils and fats. It is miscible in any ratio and will not cause layering or precipitation, thus exhibiting excellent solubility.

[0011] In a preferred embodiment of the extraction method described in this invention, the oily solvent includes at least one selected from almond oil, sunflower oil, caprylic / capric triglycerides, olive oil, grapeseed oil, low-erucic acid rapeseed oil, and sedge oil. This invention experimentally demonstrates that using the above-mentioned oily solvents as extraction reagents can yield an oil-soluble camellia extract with excellent antioxidant properties, while also exhibiting good solubility in the oily solvent.

[0012] In a preferred embodiment of the extraction method of the present invention, the mass ratio of camellia flowers to oily solvent is 1:(7.5-40). The present invention optimizes the material-liquid ratio (camellia flowers: oily solvent) in the extraction method using a controlled variable method, and the results show that a camellia flower extract with excellent antioxidant properties can be extracted within a mass ratio range of 1:7.5 to 1:40.

[0013] In a preferred embodiment of the extraction method of the present invention, the mass ratio of camellia flowers to oily solvent is camellia flowers: oily solvent = 1:(15-35).

[0014] In a preferred embodiment of the extraction method of the present invention, the mass ratio of camellia flowers to oily solvent is camellia flowers: oily solvent = 1:(20-30).

[0015] In a preferred embodiment of the extraction method of the present invention, the mass ratio of camellia flowers to oily solvent is camellia flowers: oily solvent = 1:25.

[0016] In a preferred embodiment of the extraction method described in this invention, the heating extraction is performed at 75-115℃ for 1.5-4 hours. This invention optimizes the extraction temperature and extraction time using a controlled variable method, and the results show that the camellia extract exhibits better antioxidant properties when the extraction temperature is 75-115℃ and the extraction time is 1.5-4 hours.

[0017] In a preferred embodiment of the extraction method of the present invention, the temperature of the heating extraction is 80-115℃.

[0018] In a preferred embodiment of the extraction method described in this invention, the temperature for the heating extraction is 85-115°C.

[0019] In a preferred embodiment of the extraction method described in this invention, the heating extraction temperature is 90-115℃. Experiments have shown that when the extraction temperature is 90-115℃, the camellia extract diluted by half still exhibits a DPPH inhibition rate of over 50%, indicating that the camellia extract obtained by heating at 90-115℃ has superior antioxidant properties. Furthermore, experiments have confirmed that the camellia extract obtained by heating at 115℃ exhibits the best antioxidant properties.

[0020] In a preferred embodiment of the extraction method of the present invention, the temperature of the heating extraction is 95-110℃.

[0021] In a preferred embodiment of the extraction method of the present invention, the temperature of the heating extraction is 100-105℃.

[0022] In a preferred embodiment of the extraction method described in this invention, the heating extraction time is 2-3.5 hours. Experiments conducted by this invention have shown that when the heating extraction time is 2-3.5 hours, the DPPH inhibition rate of the camellia extract reaches over 83%.

[0023] In a preferred embodiment of the extraction method described in this invention, the heating extraction time is 2.5 hours. The camellia extract exhibits the best antioxidant properties when the heating extraction time is 2.5 hours.

[0024] As a preferred embodiment of the extraction method of the present invention, the oil-soluble camellia extract obtained by heating extraction is filtered to obtain an oil-soluble camellia extract filtrate.

[0025] As a preferred embodiment of the extraction method of the present invention, the oil-soluble camellia extract obtained by heating extraction is centrifuged and filtered to obtain an oil-soluble camellia extract filtrate.

[0026] As a preferred embodiment of the extraction method of the present invention, the filtration process includes, but is not limited to, at least one of gauze filtration, passing through a 200-350 mesh sieve, and passing through a filter membrane with a pore size of 0.2-45 μm.

[0027] In a preferred embodiment of the extraction method described in this invention, a filter aid may be added during the filtration process.

[0028] In a preferred embodiment of the extraction method described in this invention, the filter aid includes, but is not limited to, at least one of diatomaceous earth, perlite, cellulose, asbestos, graphite powder, sawdust, magnesium oxide, gypsum, activated carbon, and acidic clay. During filtration, the filter aid can be spread evenly on a filter membrane before filtration. The filter aid can first adsorb impurities in the oil-soluble camellia extract, and then remove remaining impurities and microorganisms through filtration via the filter membrane.

[0029] Secondly, the present invention provides an oil-soluble camellia extract, which is mainly prepared by the above-mentioned extraction method.

[0030] Thirdly, this invention provides the application of the above-mentioned oil-soluble camellia extract in the preparation of cosmetics. The oil-soluble camellia extract of this invention can be stably dispersed in oil-phase cosmetics, maximizing the activity of the camellia extract while avoiding phenomena such as low stability and easy stratification in the oil phase system, further broadening the applications of camellia extract.

[0031] As a preferred embodiment of the application described in this invention, the dosage form of the cosmetic includes at least one of creams, lotions, aqueous solutions, gels, oils, powders, block powders or solids, patches, films, and freeze-dried products.

[0032] In a preferred embodiment of the application described in this invention, the oil-soluble camellia extract in the cosmetic contains 0.1-15% by weight.

[0033] In a preferred embodiment of the application described in this invention, the oil-soluble camellia extract in the cosmetic contains 0.5-10% by weight.

[0034] In a preferred embodiment of the application described in this invention, the oil-soluble camellia extract in the cosmetic is 1-5% by weight.

[0035] As a preferred embodiment of the application described in this invention, the oil-soluble camellia extract can be used in cosmetics with an oil phase system.

[0036] As a preferred embodiment of the application described in this invention, the oily agent includes, but is not limited to, at least one of almond oil, sunflower oil, caprylic / capric triglycerides, olive oil, grapeseed oil, low-erucic acid rapeseed oil, and sedge oil.

[0037] Fourthly, this invention provides an anti-wrinkle composition comprising the aforementioned oil-soluble camellia extract, ginseng flower oil, and snow lotus extract oil, wherein the weight ratio of the oil-soluble camellia extract: ginseng flower oil: snow lotus extract oil is (0.6-8): (0.6-8): 1. This invention, through free radical scavenging experiments and cell experiments, has found that the oil-soluble camellia extract, ginseng flower oil, and snow lotus extract oil have synergistic antioxidant and anti-wrinkle effects. The excellent antioxidant capacity of the oil-soluble camellia extract and ginseng flower oil, combined with the collagen-promoting effect of snow lotus extract oil, can synergistically combat wrinkles from both free radical scavenging and skin structure repair aspects, thus endowing the anti-wrinkle composition with both antioxidant and anti-wrinkle effects.

[0038] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (0.6-8): (0.6-8): 1.

[0039] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (0.66-6): (0.66-6): 1.

[0040] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (1.33-5): (1.33-5): 1.

[0041] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (2-4): (2-4): 1.

[0042] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (2.5-3): (2.5-3): 1.

[0043] As a preferred embodiment of the anti-wrinkle composition of the present invention, the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract in the anti-wrinkle composition is 6:4:1.

[0044] Fifthly, the present invention provides the use of the above-described anti-wrinkle composition in the preparation of antioxidant and / or anti-wrinkle articles.

[0045] As a preferred embodiment of the application described in this invention, the article includes at least one of pharmaceuticals, food, and cosmetics.

[0046] As a preferred embodiment of the application described in this invention, the application includes at least one of the following (1) to (6):

[0047] (1) The application of the anti-wrinkle composition of the oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in the weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = (0.6-8): (0.6-8): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0048] (2) The application of the anti-wrinkle composition wherein the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (0.66-6): (0.66-6): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0049] (3) The application of the anti-wrinkle composition of the oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in the preparation of antioxidant and / or anti-wrinkle products with the weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = (1.33-5): (1.33-5): 1;

[0050] (4) The application of the anti-wrinkle composition of oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in the weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = (2-4): (2-4): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0051] (5) The application of the anti-wrinkle composition of the oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in the weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = (2.5-3): (2.5-3): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0052] (6) The application of the anti-wrinkle composition wherein the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract is oil-soluble camellia extract: ginseng flower oil: snow lotus extract = (0.6-5.9): (0.6-5.9): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0053] (7) The application of the anti-wrinkle composition of the oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in the weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = (6.1-8): (6.1-8): 1 in the preparation of antioxidant and / or anti-wrinkle products;

[0054] (8) The application of the anti-wrinkle composition of oil-soluble camellia extract, ginseng flower oil and snow lotus essence oil in a weight ratio of oil-soluble camellia extract: ginseng flower oil: snow lotus essence oil = 6:4:1 in the preparation of antioxidant and / or anti-wrinkle products.

[0055] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0056] (1) The oil-soluble camellia extract obtained by the present invention using oily solvent as extraction reagent has high solubility in various oily reagents such as oils and fats. It is miscible in any ratio and will not cause layering or precipitation, thus exhibiting excellent solubility.

[0057] (2) This invention, through free radical scavenging experiments, discovered that oil-soluble camellia extract, ginseng flower oil, and snow lotus extract have synergistic antioxidant effects. The excellent antioxidant capacity of oil-soluble camellia extract and ginseng flower oil, combined with the collagen-promoting effect of snow lotus extract, can synergistically reduce wrinkles from both free radical scavenging and skin structure repair aspects, thus endowing the anti-wrinkle composition with both antioxidant and anti-wrinkle effects. Attached Figure Description

[0058] Figure 1 This illustrates the effect of different treatment groups on cell COL1 content in Example 2 of the present invention.

[0059] Figure 2 This illustrates the effect of different treatment groups on ELN content in cells in Example 2 of the present invention.

[0060] Figure 3 The effect of different treatment groups on the MMP1 content of cells in Example 2 of the present invention. Detailed Implementation

[0061] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0062] Unless otherwise specified, all other materials and reagents used in the examples are commercially available.

[0063] The techniques not described in detail in the following examples, comparative examples and effect examples are all commonly used techniques in the field. For reference, please refer to "Molecular Biology Experiment Manual" (Ma Wenli, People's Military Medical Publishing House), "Molecular Biology Experiment (Second Edition)" (Zhejiang University Press), and "Cell Biology Experiment" (Yang Hongbing, Hou Lixia, Zhang Yuxi, Higher Education Press).

[0064] The camellia pollen used in the following examples, comparative examples, and effect examples can be obtained from commercially available sources, and its source is not specifically limited.

[0065] In the following examples, comparative examples, and effect examples, the free radical scavenging rate (DPPH inhibition rate) test method is as follows:

[0066] Prepare a 0.2 mmol / L DPPH solution using anhydrous ethanol as the solvent. Mix 2 mL of the DPPH anhydrous ethanol solution with 2 mL of sample solutions of different concentrations, incubate at room temperature in the dark for 30 min, and measure the absorbance at 517 nm (A1). Replace the DPPH anhydrous ethanol solution with 2 mL of anhydrous ethanol and measure the absorbance at 517 nm (A2). Replace the sample solution with 2 mL of substrate solvent and measure the absorbance at 517 nm (A0). Perform three parallel experiments. Use a pipette to transfer 200 μL of the solution to a 96-well plate and measure the absorbance at 517 nm using a microplate reader.

[0067] The formula for calculating DPPH free radical scavenging rate (R1,%) is as follows:

[0068]

[0069] The ginseng flower oil described in the following examples, comparative examples, and effect examples consists of 0.5 wt% ginseng flower extract and 99.5 wt% triglyceride (ethylhexanoate). The ginseng flower extract can be purchased from commercial sources or extracted by oneself (through supercritical extraction). In addition, the ginseng flower oil can be prepared by replacing camellia with ginseng flower and GTCC with triglyceride (ethylhexanoate) according to the method in Example 3 below. The mass ratio of ginseng flower to triglyceride (ethylhexanoate) is ginseng flower: triglyceride (ethylhexanoate) = 1:200.

[0070] The snow lotus extract oil was purchased from Guangzhou Runhua Life Science Co., Ltd., with product number R55-035 and batch number 2411055503502.

[0071] Example 1

[0072] To screen suitable solvents for preparing oil-soluble camellia extracts, different types of plants were used as solvents to extract oil-soluble camellia extracts. The specific methods are as follows:

[0073] 1g of camellia pollen was mixed with 7.5mL of extraction solvent and extracted at 75℃ for 3h to obtain an oil-soluble camellia extract.

[0074] The oil-soluble camellia extract was centrifuged after passing through a 325-mesh sieve. The supernatant was then passed through a filter membrane with a pore size of 0.22 μm to obtain the camellia extract filtrate. The DPPH inhibition rate of the obtained camellia extract filtrate was measured. At the same time, 1 mL of the camellia extract filtrate was mixed with 9 mL of glycerol tri(ethylhexanoate) ester or squalane to determine the oil solubility of the obtained camellia extract filtrate. The types of solvents and test results are shown in Table 1.

[0075] Table 1. DPPH inhibition rate and solubility of camellia extract filtrates obtained by different solvent extraction methods.

[0076]

[0077] As shown in Table 1, camellia extracts soluble in oily reagents can be obtained using almond oil, coconut oil, sunflower oil, caprylic / capric triglyceride, olive oil, grapeseed oil, low-erucic acid rapeseed oil, and sedge oil as extraction solvents. Except for coconut oil, the DPPH inhibition rate of all camellia extracts is above 80%, with caprylic / capric triglyceride (GTCC) showing the highest DPPH inhibition rate. This indicates that using oily reagents as extraction solvents does not affect the antioxidant activity of camellia extracts. In summary, using oily reagents as extraction solvents to prepare oil-soluble camellia extracts is highly feasible while preserving the activity of the camellia extract. Furthermore, no layering or other phenomena occur when camellia extracts are mixed with triglycerides (ethylhexanoate) or squalane in any proportion, indicating that camellia extracts have excellent solubility in oily reagents such as fats. Considering both antioxidant activity and solubility, the following examples, comparative examples, and effect examples all use GTCC as the extraction solvent to prepare oil-soluble camellia extracts and oil-soluble camellia extract filtrates.

[0078] Example 2

[0079] To explore the optimal parameter adjustment for the extraction method of oil-soluble camellia extract, the extraction temperature, time, and ratio of camellia to oily solvent (hereinafter referred to as the solid-liquid ratio) were screened. Simultaneously, orthogonal experiments were conducted using temperature, time, and solid-liquid ratio to screen for the optimal parameter combination. The specific scheme is as follows:

[0080] 1. Heating extraction temperature.

[0081] 1g of camellia flowers were mixed with 7.5g of GTCC and then heated at different temperatures for 3 hours to obtain an oil-soluble camellia flower extract.

[0082] The oil-soluble camellia extract was centrifuged after passing through a 325-mesh sieve. The supernatant was then passed through a filter membrane with a pore size of 0.22 μm to obtain the camellia extract filtrate. The obtained camellia extract filtrate was mixed with GTCC and diluted to a 50 v / v% solution. The DPPH inhibition rate of the diluted camellia extract filtrate was then measured. The results are shown in Table 2.

[0083] Table 2. Results of DPPH inhibition rate determination of oil-soluble camellia extract at different heating temperatures.

[0084] Serial Number Extraction temperature (°C) DPPH inhibition rate (%) 1 75 44.73 2 80 34.20 3 85 45.79 4 90 51.41 5 95 55.05 6 100 55.40 7 105 56.45 8 110 61.02 9 115 54.17

[0085] As shown in Table 2, the DPPH inhibition rate of the diluted camellia extract filtrate increased with increasing heating temperature, until the DPPH inhibition rate was highest at a heating temperature of 110℃. Taking all factors into consideration, 90-115℃ was selected as the preferred heating extraction temperature.

[0086] 2. Heating extraction time.

[0087] 1g of camellia flowers were mixed with 7.5g of GTCC and extracted at 115℃ for 1.5-4h to obtain an oil-soluble camellia flower extract.

[0088] The oil-soluble camellia extract was centrifuged after passing through a 325-mesh sieve. The supernatant was then passed through a filter membrane with a pore size of 0.22 μm to obtain the camellia extract filtrate. The obtained camellia extract filtrate was mixed with GTCC and diluted to a 50 v / v% solution. The DPPH inhibition rate of the diluted camellia extract filtrate was then measured. The results are shown in Table 3.

[0089] Table 3. Results of DPPH inhibition rate determination of oil-soluble camellia extract at different heating times.

[0090] Serial Number Time (h) DPPH inhibition rate (%) 1 1.5 50.33 2 2 54.24 3 2.5 58.56 4 3 58.20 5 3.5 57.79 6 4 56.25

[0091] As shown in Table 3, the DPPH inhibition rate of the diluted camellia extract filtrate increased with increasing heating time, until the highest DPPH inhibition rate was achieved at a heating time of 2.5 h. Therefore, 1.5-4 h can be used as the heating extraction time.

[0092] 3. Material-to-liquid ratio.

[0093] Camellia flowers were mixed with GTCC and extracted at 115℃ for 3 hours to obtain an oil-soluble camellia flower extract.

[0094] The oil-soluble camellia extract was centrifuged after passing through a 325-mesh sieve. The supernatant was then passed through a filter membrane with a pore size of 0.22 μm to obtain the camellia extract filtrate. The obtained camellia extract filtrate was diluted with GTCC at a weight ratio of 1:40 (camellia extract filtrate: GTCC). The DPPH inhibition rate of the diluted camellia extract filtrate was measured, and the results are shown in Table 4.

[0095] Table 4. Results of DPPH inhibition rate determination of oil-soluble camellia extract at different material-to-liquid ratios.

[0096] Serial Number Material-to-liquid ratio (camellia:GTCC, g:g) DPPH inhibition rate (%) 1 1:5 26.92 2 1:10 31.82 3 1:15 39.25 4 1:20 59.38 5 1:25 58.73 6 1:30 58.74 7 1:35 46.08 8 1:40 53.08

[0097] As shown in Table 4, the DPPH inhibition rate of the diluted camellia extract filtrate obtained with different material-to-liquid ratios is different. The DPPH inhibition rate of 1:15 to 1:40 is higher than that of 1:5 and 1:10. Therefore, a material-to-liquid ratio of 1:(15-40) is the preferred parameter condition for the extraction method.

[0098] In summary, the parameters for the extraction method of oil-soluble camellia extract include a material-to-liquid ratio of 1:(7.5-40) (preferably 1:(15-40)), an extraction temperature of 75-115℃, and an extraction time of 1.5-4h.

[0099] 4. Orthogonal experiment.

[0100] Based on the single-factor experimental design, a 3-factor, 3-level L9(3) experiment... 3 An orthogonal experiment was conducted, and the final optimized extraction method was obtained based on the orthogonal experiment results. The extraction method was carried out according to the methods mentioned in 1-3 above. The DPPH inhibition rate was measured using a solution diluted to 50 v / v%. The orthogonal experiment is shown in Table 5. The temperatures were 100℃ (A1), 105℃ (A2), and 110℃ (A3), the times were 2.5h (B1), 3h (B2), and 3.5h (B3), and the material-to-liquid ratios were 15g / g (C1), 20g / g (C2), and 25g / g (C3).

[0101] Table 5. Results of orthogonal experiments on various parameters of oil-soluble camellia extract.

[0102]

[0103] As shown in Table 5, R j A higher value indicates a greater influence of this factor on the antioxidant capacity of the camellia extract filtrate. Therefore, the order of influence of the three factors on the antioxidant capacity of camellia extract is: temperature > solid-liquid ratio > time; according to T... 1j T 2j and T 3j The optimal solution is A3B3C3.

[0104] Repeatability tests were conducted according to the optimal scheme (A3B3C3). Camellia flowers and GTCC were mixed at a material-liquid ratio of 1g:25g and extracted at 110℃ for 3.5h to obtain an oil-soluble camellia flower extract. After centrifugation and filtration, the extract was diluted to 50v / v% and the DPPH inhibition rates were measured to be 76.52%, 75.78%, and 76.33%, respectively. This demonstrates that the optimal process is repeatable and the results are reliable.

[0105] Example 3

[0106] Example 3 provides an oil-soluble camellia extract and its extraction method. The extraction method mainly involves mixing camellia flowers and GTCC at a ratio of 1g camellia flowers to 25g GTCC and then heating and extracting at 115°C for 3.5 hours to obtain the oil-soluble camellia extract.

[0107] The oil-soluble camellia extract obtained above can be post-processed as follows: the oil-soluble camellia extract is passed through a 325-mesh sieve and centrifuged. The supernatant is then passed through a filter membrane with a pore size of 0.22 μm to obtain the camellia extract filtrate.

[0108] Examples 4-10 and Comparative Examples 1-9

[0109] Examples 4-10 and Comparative Examples 1-9 respectively provide an anti-wrinkle composition and its preparation method. The components and their amounts in the compositions are shown in Table 6. The preparation method mainly involves mixing oil-soluble camellia extract, ginseng flower oil, and snow lotus extract to obtain the anti-wrinkle composition. The oil-soluble camellia extract used is the camellia extract filtrate obtained in Example 3, and the mass percentage of camellia extract to GTCC in the camellia extract filtrate is approximately camellia extract:GTCC = 4:96. The ginseng flower oil used is prepared according to the method of Example 3, and the mass percentage of ginseng flower extract to triglyceride is approximately ginseng flower extract:triglyceride = 0.5:99.5.

[0110] Table 6. Components and dosages of different anti-wrinkle compositions (mass ratio of the three components per 100 parts by weight of the anti-wrinkle composition)

[0111]

[0112]

[0113] Example 11

[0114] Example 11 provides an oil-soluble camellia extract and its extraction method. The extraction method mainly involves mixing camellia flowers and GTCC at a ratio of 1g camellia flowers to 25g GTCC and then heating and extracting at 115°C for 3.5 hours to obtain the oil-soluble camellia extract.

[0115] The oil-soluble camellia extract obtained above can be post-processed as follows: after the oil-soluble camellia extract is allowed to stand and separate into layers, the supernatant is taken and filtered through a filter membrane with a pore size of 0.2 μm and pre-coated with diatomaceous earth filter aid to obtain camellia extract filtrate.

[0116] Comparative Example 10

[0117] Comparative Example 10 provides an anti-wrinkle composition and its preparation method. The components and their amounts in the composition are similar to those in Example 4, except that the oil-soluble camellia extract is replaced with aloe vera flower oil, while the remaining components and their amounts remain unchanged. The preparation method is the same as in Example 4, and the aloe vera flower oil is extracted according to the parameters and conditions of the extraction method described in Example 3.

[0118] Comparative Example 11

[0119] Comparative Example 11 provides an anti-wrinkle composition and its preparation method. The components and their amounts in the composition are similar to those in Example 4, except that the oil-soluble camellia extract is replaced with rose oil, while the remaining components and their amounts remain unchanged. The preparation method is the same as in Example 4, and the rose oil is extracted according to the parameters and conditions of the extraction method described in Example 3.

[0120] Example 1

[0121] To verify the antioxidant properties of the anti-wrinkle compositions obtained in Examples 4-10 and Comparative Examples 1-11, the DPPH inhibition rate of the above anti-wrinkle compositions was tested, and the results are shown in Table 7.

[0122] Table 7. Results of antioxidant performance tests for different anti-wrinkle compositions

[0123]

[0124] As shown in Table 7, the DPPH scavenging rates of the anti-wrinkle compositions obtained in the examples were all above 88%, significantly higher than those in the comparative examples, indicating that the anti-wrinkle compositions of the present invention have superior antioxidant effects. Compared with the examples, the DPPH scavenging rates of comparative examples 1-9 decreased significantly, indicating that only when oil-soluble camellia extract, ginseng flower oil, and snow lotus extract are combined in a specific ratio range to form an anti-wrinkle composition can excellent antioxidant effects be achieved, and the three have a synergistic effect. Compared with the examples, the DPPH scavenging rates of comparative examples 10-11 decreased significantly, indicating that even using raw materials similar to oil-soluble camellia extract to form an anti-wrinkle composition cannot give it excellent antioxidant properties.

[0125] Example 2

[0126] The anti-wrinkle efficacy of the anti-wrinkle compositions obtained in Example 4 and Comparative Examples 8 and 9 was tested. Specifically, cells in the logarithmic growth phase were selected and analyzed at a concentration of 2.5 × 10⁻⁶ cells / year. 5 Seed cells at a density of 800 μL / mL into 12-well plates and incubate overnight in an incubator (37°C, 5% CO2). When the cell deposition rate reaches 50-60%, aspirate the culture medium from the wells and replace it with pre-cooled PBS (800 μL per well). Place the plates in a UV curing chamber for UVA irradiation (60 min, dose pre-set to 2.78 mW / cm²). 2 After irradiation, PBS was aspirated and the cells were administered to groups of 500 μL each, with three replicates per group. After 48 h of culture, the cell culture supernatant was collected into 200 μL EP tubes (Note: the amount of sample collected was determined according to the detection indicators). After collection, the samples used for COLI, MMP1 and ELN content detection were frozen and stored at -80℃. The specific test methods were performed in accordance with the ELISA kit instructions.

[0127] The experimental design was as follows: blank control group (no UV irradiation); model group (UV irradiation only); positive control group (UV irradiation, administration of 50 μg / mL vitamin C); sample group (UV irradiation, administration of 5 v / v% of Example 4, Comparative Example 8 or Comparative Example 9, solvent: GTCC). Test results are shown below. Figure 1-3 .

[0128] Type I collagen is one of the main components of the dermal extracellular matrix. It is synthesized intracellularly by dermal fibroblasts, secreted extracellularly, and polymerized to form collagen fibers after the telomeres separate under the influence of terminal procollagen peptides. Elastin plays a crucial role in maintaining skin elasticity, giving tissue elasticity and expansion capacity, providing resilience to the extracellular matrix. Elastin acts as a scaffold, helping to repair skin elasticity, allowing the skin to stretch and fold, and is responsible for maintaining and supporting skin elasticity. Matrix metalloproteinases (MMPs) belong to the zinc-dependent endopeptidase family, one of the most important protease families, capable of degrading almost all components of the extracellular matrix, as well as myelin, growth factors, cytokines, and cell adhesion molecules.

[0129] like Figure 1 As shown, compared with the model group, the COL1 content of the anti-wrinkle compositions obtained in Example 4 and Comparative Examples 8-9 was significantly increased, with Example 4 being superior to the positive control group; Figure 2 As shown, compared with the model group, the ELN content of the anti-wrinkle compositions obtained in Example 4 and Comparative Example 9 was significantly higher than that in the model group, with Example 4 being superior to the positive control group; as Figure 3 As shown, compared with the model group, the MMP1 content of the anti-wrinkle compositions obtained in Examples 4 and Comparative Examples 8-9 was significantly lower than that in the model group, with the content in Example 4 being closest to the control group. These results indicate that the anti-wrinkle compositions of the present invention have the effects of increasing type I collagen and elastin, and reducing the expression level of matrix metalloproteinases, thus exhibiting superior anti-wrinkle efficacy.

[0130] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A method for extracting an oil-soluble camellia flower extract, characterized in that, Camellia flowers were mixed with an oily solvent and heated to extract the oil-soluble camellia flower extract. The oily solvent is caprylic / capric triglyceride; The heating extraction was performed at 110°C for 3.5 hours. The mass ratio of camellia flowers to oily solvent is 1:

25.

2. The extraction method as described in claim 1, characterized in that, The oil-soluble camellia extract obtained by heating and extraction is filtered to obtain an oil-soluble camellia extract filtrate.

3. An oil-soluble camellia flower extract, characterized in that, It is prepared by the extraction method described in any one of claims 1-2.

4. The application of the oil-soluble camellia extract as described in claim 3 in the preparation of cosmetics.

5. An anti-wrinkle composition, characterized in that, It includes oil-soluble camellia extract, ginseng flower oil and snow lotus extract oil, wherein the weight ratio of the oil-soluble camellia extract, ginseng flower oil and snow lotus extract oil is oil-soluble camellia extract: ginseng flower oil: snow lotus extract oil = (0.6-8): (0.6-8): 1; The extraction method of the oil-soluble camellia extract is as follows: Camellia flowers are mixed with caprylic / capric triglycerides and extracted at 115°C for 3.5 h to obtain the oil-soluble camellia extract. The mass ratio of camellia flowers to caprylic / capric triglycerides is camellia flowers: caprylic / capric triglycerides = 1:

25. The method for extracting ginseng flower oil is as follows: ginseng flowers are mixed with triglycerides (ethylhexanoate) and extracted at 115°C for 3.5 hours to obtain ginseng flower oil. The mass ratio of ginseng flowers to triglycerides (ethylhexanoate) is ginseng flowers:triglycerides (ethylhexanoate) = 1:

200. The snow lotus extract oil is sourced from Guangzhou Runhua Life Science Co., Ltd.

6. The anti-wrinkle composition according to claim 5, characterized in that, The weight ratio of the oil-soluble camellia extract, ginseng flower oil, and snow lotus extract in the anti-wrinkle composition is 6:4:

1.

7. The use of the anti-wrinkle composition as described in claim 5 or 6 in the preparation of antioxidant and / or anti-wrinkle cosmetics.

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