Botanical attractant for thrips hawaiiensis in banana field and application of botanical attractant

Abstract

The invention discloses a botanical attractant for thrips hawaiiensis in a banana field and application of the botanical attractant. According to the application, alpha-pinene, caryophyllene, DL-limonene, beta-pinene and D-limonene which have a remarkable attracting effect on thrips hawaiiensis are separated and identified from banana flower buds which are host plants of the thrips hawaiiensis, and effective concentrations of the compounds with the attracting effect on the thrips hawaiiensis are screened out; the five compounds are mixed according to a certain volume ratio to prepare the thrips hawaiiensis luring composition. Indoor and outdoor experiments prove that the trapping composition provided by the invention has remarkable trapping activity on thrips hawaiiensis in a banana field. The attractant provided by the invention is derived from plants, is simple to prepare, is environment-friendly, is non-toxic to people and livestock, and has a good popularization and application prospect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of green pest control, and particularly relates to a plant source attractant for yellow chested thrips in banana plantations and application thereof. BACKGROUND

[0002] Yellow chested thrips Thrips hawaiiensis Yellow chested thrips (Morgan) belongs to Thysanoptera, Thripidae, is widely distributed in tropical and subtropical regions. In China, it is mainly distributed in Hainan, Guangdong, Guangxi, Fujian, Yunnan and Taiwan and other tropical and subtropical regions, and is also found in the south of Huaihe River. There are more than 10 generations in a year, more than 20 generations in a year in tropical regions, and it can occur all year round in greenhouses. Adult thrips overwinter in dead branches and leaves, and start to cause damage in early March of the next year. Female adults insert their ovipositors into young tissues, petals or corolla epidermis when laying eggs, and lay eggs under the epidermis or semi-exposed. Adult and nymph thrips hide in flowers and fly away when startled. They mainly damage the surface of plants and fruits with a rasping stylet, and suck the sap of flowers, ovaries and young fruits. High temperature and drought are conducive to the occurrence of yellow chested thrips, and the most suitable conditions for its development are temperature 25-32℃ and relative humidity 30%-60%, and it occurs less in rainy seasons. Yellow chested thrips has many host crops, and after tropical fruits such as bananas and mangoes are damaged, the cells around the eggs grow abnormally and swell, the damaged fruit epidermis tissue proliferates and suberizes, forming small black spots, which affects the appearance and quality of the fruits; after flowers such as orchids, chrysanthemums, roses and lilies are damaged, grayish white dot-shaped feeding marks and oviposition marks appear on the petals, and the petals curl and cannot open smoothly; after vegetables such as various melons, cowpeas, green beans, peppers, eggplants and cruciferous vegetables are damaged, the flowers leave grayish white dot-shaped feeding marks, affecting fruit setting. Yellow chested thrips has strong adaptability and concealment, extremely strong reproductive capacity and short generation cycle, and is difficult to detect in the early stage of damage. Once it migrates to suitable crops, the thrips population rapidly increases in a short period of time, and it is difficult to effectively control in production.

[0003] Spraying chemical pesticides is the main method to control T. castaneum in production. The commonly used pesticides include spirotetramat, spinetoram, imidacloprid, thiamethoxam and lambda-cyhalothrin, etc. However, T. castaneum often hides in the flower bud, and the protection of bracts reduces the contact opportunity between the insect body and the pesticide, making it difficult to achieve effective killing effect. In addition, for banana crops, other researchers have tried to inject pesticides into flower buds and pseudostems, which have good control effect on T. castaneum, but there are problems such as time-consuming, difficult operation, easy to cause damage to the plant, etc. Frequent and large-scale use of chemical pesticides can easily lead to the development of pesticide resistance in T. castaneum. Studies have shown that T. castaneum has developed resistance to organophosphorus insecticides, imidacloprid, etc. Moreover, the large-scale use of pesticides also increases the risk of pesticide residues in banana products. The problems of pesticide resistance, food safety and environmental pollution caused by chemical pesticides have attracted more and more attention, and people are constantly seeking new and environmentally friendly control measures and technologies.

[0004] Using insect positioning signals of host plants as a control means is a current research hotspot. Previous studies have shown that the behavior of pests selecting host plants is influenced by a variety of factors, including host volatiles, color, shape, degree of tenderness, insect memory and learning experience, etc. Using the color and odor of host plants to develop corresponding control measures is an environmentally friendly method. A large number of studies have shown that thrips can locate hosts through plant color and odor. Researchers use the thrips' tendency to achieve the purpose of monitoring and killing thrips populations by using color plates and color plates + attractant modes. Beavers et al. found that thrips on citrus are more sensitive to white, and white sticky boards can effectively increase the number of thrips (Scirtothrips citri) captured on citrus. Bian et al. found that yellow sticky insect boards can effectively capture yellow tea thrips and striped tea thrips in tea gardens. Tang and Mao et al. found that blue sticky insect boards can effectively monitor the silk thrips and flower thrips of legume crops. The combination of color plates and thrips attractants can improve the attractant efficiency of color plates. Imai et al. added p-methane phenylamine and methyl p-aminobenzoate to the insect traps to significantly improve the attractant efficiency of T. castaneum. Abdullah et al. added terpineol to the sticky insect board to significantly increase the number of thrips captured. Studies on the occurrence of T. castaneum in banana plantations have shown that flower buds are the key to T. castaneum's selection of migration, and further studies have shown that flower bud volatiles are the key factors to attract T. castaneum to migrate.

[0005] There are usually 2-3 species of thrips on a host plant, and even more species can be present simultaneously. For banana crops, existing research shows that only one species of thrips, the yellow chest thrips, is present and causes harm in banana flower buds, and the proportion of female adult yellow chest thrips in the banana garden is high, and only female adults can cause harm to bananas. Previous monitoring results show that yellow chest thrips nymphs have a small range of activity and do not migrate, and the effect of attractants and blue sticky insect boards on them is small. Based on this specific harm characteristic, the inventors speculate that there is a specific component in banana flower bud volatiles that has strong attractant activity for yellow chest thrips, and this component may match its olfactory perception system (such as specific odor binding proteins) to become a key chemical signal driving yellow chest thrips to select banana flower buds as hosts. Based on this hypothesis, the inventors' team collected and used a gas chromatograph mass spectrometer to determine banana flower bud volatiles, selected single pure compounds of volatile components, determined the behavioral selection of yellow chest thrips adults to different compounds indoors, and further verified the attractant activity of the compounds in the field. A single compound with good attractant activity for yellow chest thrips was screened, and the single compound was combined in different concentrations and with different compounds. Through indoor bioassay tests and field attractant activity verification tests, the compound combination and concentration combination with strong attractant activity were determined, and a yellow chest thrips attractant and its application method were developed. The patent with the publication number CN107996541A discloses an attractant for banana yellow chest thrips, which is orange flower alcohol, ethyl nicotinate, rosewood essential oil, verbena essential oil, or mace essential oil.

[0006] According to the search, there is currently no research on using alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene, and combinations thereof as a yellow chest thrips attractant in banana plantations. SUMMARY

[0007] In view of the deficiencies of the prior art, the present application provides a plant-derived attractant for yellow chest thrips in banana plantations and its application.

[0008] To solve the above technical problems, the technical solution of the present application is as follows: A plant-derived attractant for yellow chest thrips in banana plantations, the attractant being any one of alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene, or a combination thereof, the concentration of alpha-pinene being 10.0 μg / mL, caryophyllene being 10.0 μg / mL, DL-limonene being 10.0 μg / mL, D-limonene being 1.0 μg / mL, and beta-pinene being 1.0 μg / mL.

[0009] Further, the composition is any combination of the following: alpha-pinene, caryophyllene and D-limonene, or alpha-pinene, caryophyllene and beta-pinene, or alpha-pinene, DL-limonene and D-limonene, or alpha-pinene, D-limonene, beta-pinene, or caryophyllene, DL-limonene, D-limonene, or alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene in any four or five combinations.

[0010] Preferably, the caryophyllene, DL-limonene, beta-pinene, D-limonene is in a volume ratio of 1:1:0.1:0.1, or the alpha-pinene, caryophyllene, DL-limonene, D-limonene is in a volume ratio of 1:1:1:0.1, or the alpha-pinene, caryophyllene, DL-limonene, beta-pinene is in a volume ratio of 1:1:1:0.1, or the alpha-pinene, DL-limonene, D-limonene, beta-pinene is in a volume ratio of 1:1:0.1:0.1, or the alpha-pinene, caryophyllene, D-limonene, beta-pinene is in a volume ratio of 1:1:0.1:0.1.

[0011] Preferably, the composition is alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene in a volume ratio of 1:1:1:0.1:0.1.

[0012] The application also protects the use of the plant-derived attractant of the banana yellow thorax thrips in the banana garden in the preparation of a product for monitoring the occurrence of the banana yellow thorax thrips in the banana garden and physical control measures.

[0013] The application also protects a method for monitoring or controlling the banana yellow thorax thrips, comprising the following steps: using the plant-derived attractant or the plant-derived attractant with any one of claims 1-4 in combination with a trapping device to trap the banana yellow thorax thrips.

[0014] Further, the trapping device is a blue sticky insect board.

[0015] Compared with the prior art, the application has the following beneficial effects: The application separates and identifies alpha-pinene, caryophyllene, DL-limonene, beta-pinene and D-limonene from the banana flower buds of the host plant of the banana yellow thorax thrips, which have a significant attraction effect on the banana yellow thorax thrips, and screens the effective concentration of each compound for attracting the banana yellow thorax thrips, and the five compounds are mixed according to a certain volume ratio to prepare an attraction composition of the banana yellow thorax thrips. Indoor and outdoor experiments prove that the attraction composition provided by the application has a significant attraction activity on the banana yellow thorax thrips. The application provides a plant-derived attractant and a method, which can be applied to monitoring the population dynamics of the banana yellow thorax thrips in the banana garden and controlling the banana yellow thorax thrips in the field by using an attraction control technology. The attractant provided by the application is derived from plants, is simple to prepare, is friendly to the environment, is non-toxic to humans and animals, and has a good application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 Selection rates of banana yellow thoracic thrips on young fruits and bracts in banana flower tissues, Note: CA represents clean air; asterisk represents significant level, *** means P<0.001, * means P<0.05, "ns" means P>0.05; the horizontal coordinate number represents selection rate; Figure 2 Behavioral selection of banana yellow thoracic thrips female adults on 7 kinds of volatile substances, Note: Figure 2 a selection rate of α-pinene, Figure 2 b selection rate of β-pinene, Figure 2 c selection rate of D-limonene, Figure 2 d selection rate of DL-limonene, Figure 2 e selection rate of β-caryophyllene, Figure 2 f selection rate of eucalyptus oil, Figure 2 g selection rate of roscinene. Asterisk represents significant level, *** means P<0.001, ** means P<0.01, * means P<0.05, "ns" means P>0.05; the horizontal coordinate number represents selection rate; Figure 3 Attracting effect of single compound combined with blue sticky board on banana yellow thoracic thrips adults in banana garden, Note: the data in the figure are mean ± standard deviation, and different lowercase letters on the column represent significant difference (P<0.05); Figure 4 Attracting effect of various different single agent combinations on banana yellow thoracic thrips adults in banana garden, Note: Figure 4 a selection rate of 5 kinds of single agent two-by-two combination, Figure 4 b selection rate of 5 kinds of single agent three-by-three combination, Figure 4 c selection rate of 5 kinds of single agent four-by-four combination, Figure 4 d selection rate of 5 kinds of single agent all combinations. Asterisk represents significant level, *** means P<0.001, ** means P<0.01, * means P<0.05, "ns" means P>0.05; the horizontal coordinate number represents selection rate; Figure 5 Attracting effect of different compound combinations combined with blue sticky board on banana yellow thoracic thrips adults in banana garden, Note: 10.0 μg / mL α-pinene (A), 10.0 μg / mL caryophyllene (B), 10.0 μg / mL DL-limonene (C), 1.0 μg / mL DL-limonene (D), 1.0 μg / mL β-pinene (E) are four-by-four combinations and five-by-five combinations according to their proportions. The data in the figure are mean ± standard deviation, and different lowercase letters on the column represent significant difference (P<0.05). DETAILED DESCRIPTION

[0017] The technical solutions of the present application will be further described in detail below in combination with the drawings and specific embodiments, but the present application is not limited to the following technical solutions.

[0018] Test site and insect source: The field test was carried out in a banana plantation in Ganzhuang Community, Yuanjiang County, Yuxi City, Yunnan Province (101°56'48"E, 23°41'30"N, 708.54 m above sea level). The yellow stern aphids in the test banana plantation were collected, and green bean seedlings were used as host plants for indoor rearing. The yellow stern aphids were fed with 10 times the solution of pure water diluted with pollen and honey (1:1) as the nutrient solution. The female aphids of the thrips that had been continuously reared for 3 generations and had consistent growth periods were selected for the test.

[0019] Test agents: n-hexane, 100 μg / mL limonene, 100 μg / mL D-limonene, 100 μg / mL α-pinene, 100 μg / mL β-pinene, 100 μg / mL 1,8-eucalyptol, 1000 μg / mL eucalyptol, 100 μg / mL β-caryophyllene purchased from Tianjin Alta Technology Co., Ltd.

[0020] Example 1: Compounds with significant attraction to yellow stern aphids were isolated and identified from different organs of banana flower buds

[0021] 1.1 Selectivity of yellow stern aphids to different organs of banana flower buds A Y-shaped olfactory instrument was used to test the selectivity of adult female yellow-breasted thrips for banana flower buds, stamens, bracts, and young fruit. The Y-shaped tube was made of transparent glass, with both arms and the base measuring 10 cm in length and 2 cm in inner diameter. The angle between the two arms was 45°. The arms were connected sequentially to an odor source bottle, a gas flow meter, a gas washing bottle (for purifying and humidifying the air), and a drying tower (containing activated carbon for air purification) via silicone tubing. The straight arm was connected to an air pump. The Y-shaped tube was placed in a dark box with a 40W incandescent lamp at the top to ensure uniform lighting and avoid the influence of inconsistent lighting on thrips selection behavior. The experimental environment was 25℃ and 70% humidity. The airflow rate was 40 ml / min. The test was divided into two groups: the first group used clean air as a control group to test the selectivity of yellow-breasted thrips for banana flower bud tissues; the second group used tissues as a control group to test the selectivity of yellow-breasted thrips for different tissues of banana flower buds. During the test, 5g of tissue sample was placed in a odorant bottle. A single female adult yellow-breasted thrips was introduced through the straight arm of a Y-shaped tube, and its selection behavior was observed and recorded. The selection criteria were as follows: if the adult thrips lingered for 15 seconds or more after crossing 2 / 3 of an arm, it was considered a selection; if no selection was made within 5 minutes, it was considered a non-selection. Ten groups were set up for each tissue experiment, with 6 thrips per group, for a total of 60 thrips. The tissue material was replaced after each group was completed. To avoid the influence of positional effects and residual odor, the Y-shaped tube was wiped with ethanol and the positions of the experimental and control groups were swapped after each thrips was tested. Selection rate (%) = (Number of yellow-breasted thrips selected from banana bud tissue or clean air / Total number of yellow-breasted thrips tested) * 100

[0022] Female adult yellow-breasted thrips exhibit a significant attraction-to-bract and attraction-to-yolk fruit appearance to banana flower buds and bracts. For example... Figure 1 As shown, with clean air as a control, both young fruits (χ² = 41.68, P < 0.001) and bracts (χ² = 18.29, P < 0.001) exhibited highly significant attraction to yellow-breasted thrips. Young fruits showed the strongest attraction, with a selection rate of 83.3%, followed by bracts at 73.3%. In experiments comparing different flower bud tissues as controls, there was no significant difference in the selection rate of female yellow-breasted thrips towards bracts and young fruits.

[0023] 1.2 Determination of volatiles in bracts and young fruit of banana flower buds According to the experimental results of the behavior response of yellow thorax thrips to different tissues of banana flower buds, the young fruits and bracts with good attraction effect were selected, and the volatile components were identified by gas chromatography-mass spectrometry. The young fruits and bracts were cut into pieces, 2.0 g of sample was placed in a 20 mL headspace bottle for solid phase microextraction, a 50 / 30 μm DVB / CAR-PDMS type extraction head was inserted, 40 ℃ equilibrium for 40 min, and the extraction head was immediately inserted into the GC-MS inlet for analysis after taking out. GC-MS is Agilent 7890 / 5975 mass spectrometer detector. Chromatographic column: 30 m DB-5MS (30 m × 0.25 mm × 0.25 μm) capillary column; temperature program: initial temperature 50 ℃, hold for 1 min, increase to 200 ℃ at 8 ℃ / min, hold for 2 min, then increase to 250 ℃ at 12 ℃ / min, hold for 4 min; the inlet temperature is kept constant at 250 ℃, the carrier gas is helium, and the constant flow rate is 1 mL / min; mass spectrometry conditions: transmission line setting is 290 ℃, mass spectrometry acquisition parameters include scanning from 50-600 m / z in electron impact (EI) mode, spectrum library NIST11.L is used for retrieval analysis, and area normalization method is used to calculate the relative content of each component.

[0024] Among the 34 substances detected in the bract volatiles, there are 16 substances with a matching degree of 90% and above and a relative content of more than 1%. Among the 31 substances detected in the young fruit volatiles, there are 15 substances with a matching degree of 90% and above and a relative content of more than 1%. There are 7 substances common to both tissues, which are α-pinene, β-pinene, caryophyllene, D-limonene, DL-limonene, ocimene and 1,8-cineole.

[0025] Table 1 Analysis results of bract and young fruit volatile substances

[0026] 1.3 Yellow thorax thrips female adults' selection behavior to common key volatiles in banana bracts and young fruits The 7 substances commonly selected by the adults of T. citrella and with relatively high content, i.e., α-pinene, β-pinene, limonene, eucalyptol, roclene, and caryophyllene, were selected for indoor tests. Standard solutions with concentrations of 0.1 μg / mL, 1.0 μg / mL, and 10.0 μg / mL were prepared. The selectivity of female adults of T. citrella to the 7 substances with different concentrations was determined. A "Y" type tube was made of transparent glass tube, with a length of 10 cm, an inner diameter of 2 cm, and an angle of 45° between the two arms. The two arms were connected with silica gel tubes in sequence, i.e., a smell source bottle, a gas flow meter, a gas washing bottle (for purifying and moistening air), a drying tower (containing activated carbon for purifying air), and a suction pump. The "Y" type tube was placed in a dark box, with a 40 W incandescent lamp installed at the top of the box to ensure uniform illumination in the box, so as to avoid the influence of uneven illumination on the selection behavior of the thrips. The temperature of the experimental environment was 25℃, and the humidity was 70%. The air flow rate was 40 ml / min. The test was divided into two groups. The first group was a control group with n-hexane, and the second group was tested with 2 μL of each sample (α-pinene, β-pinene, limonene, eucalyptol, roclene, and caryophyllene, prepared into standard solutions with concentrations of 0.1 μg / mL, 1.0 μg / mL, and 10.0 μg / mL) added to the filter paper in the sample arm of the "Y" type olfactometer. Sixty adults of T. citrella were tested for each concentration. During the test, the sample was placed in the smell source bottle, and a single female adult of T. citrella was introduced from the straight arm port of the "Y" type tube. The selection behavior was observed and recorded. The selection criteria were as follows: when the adult crossed 2 / 3 of the arm and stayed for 15 seconds or more, it was recorded as selection; if no selection was made within 5 min, it was recorded as no selection. Ten groups were set for each sample, with 6 adults in each group, i.e., a total of 60 adults. After the test of one group was completed, the test materials were replaced. In order to avoid the influence of position effect and residual odor, after the test of one adult was completed, the "Y" type tube was wiped with ethanol, and the positions of the test group and the control group were exchanged. The selection rate (%) = the number of adults of T. citrella that selected the test compound or clean air / the total number of adults of T. citrella tested × 100

[0027] The results are shown in Table 1. Figure 2As shown in Table 1, among the 7 tested substances, 10.0 μg / mL of α-pinene (χ2= 12.26, P < 0.001), 10.0 μg / mL of caryophyllene (χ2= 12.26, P < 0.001), 10.0 μg / mL of DL-limonene (χ2= 12.26, P < 0.001), 1.0 μg / mL of β-pinene (χ2= 12.26, P < 0.001), and 1.0 μg / mL of DL-limonene (χ2= 12.26, P < 0.001) had extremely significant attraction to the yellow thorax thrips. 1.0 μg / mL of 1,8-cineole had significant repellent effect, and other two concentrations of α-pinene, caryophyllene, D-limonene, DL-limonene, and 1,8-cineole had no significant effect on the yellow thorax thrips female adults (P > 0.05). 10.0 μg / mL, 1.0 μg / mL, and 0.1 μg / mL of rosmarine had no significant effect on the yellow thorax thrips female adults.

[0028] 1.4 Attraction of 5 active volatile substances to the yellow thorax thrips in banana plantations The 5 compounds with attraction to the yellow thorax thrips female adults were selected and used in combination with blue sticky boards to evaluate the attraction to the yellow thorax thrips in banana plantations. Standard solutions of corresponding concentrations were prepared using n-hexane as a solvent. Before the field test, 10 mL of the standard solution was taken into a 15 mL centrifuge tube, 10 holes were punched on the tube cap with a No. 4 insect needle to allow the volatiles to be emitted, and the centrifuge tube was attached to the bottom of the blue sticky board with the opening upward. In the banana plantation, plants with 2-3 comb-shaped opened bracts were selected for marking, and the sticky boards with volatiles were placed on the banana pseudostems at the same height as the flower buds. The control treatment was the sticky board without volatiles. Each treatment was repeated 3 times, and each repeat had 3 plants with buds. After 5 days, the number of thrips female and male adults on the sticky boards was counted using a hand magnifier.

[0029] The number of yellow thorax thrips female adults captured by 5 volatiles in combination with blue sticky boards is shown in Table 2. Figure 3 As shown in Table 2, n-hexane had no significant difference compared with CK, and the number of yellow thorax thrips female adults treated with the test compounds was significantly higher than that of CK, among which the number of yellow thorax thrips female adults captured by 10.0 μg / mL of α-pinene was the highest and significantly higher than that of other substances.

[0030] Example 2 Effect of plant-derived attractants of the yellow thorax thrips The odor played a key role in the process of Thrips flavus selecting and positioning banana flower buds. Further analysis of the volatiles from bracts and young fruits by GC-MS showed that 34 substances were detected in the bracts and 31 substances were detected in the young fruits. Seven substances with higher relative content were common to both, which were α-pinene, β-pinene, D-limonene, DL-limonene, ocimene, 1,8-cineole, and caryophyllene. The behavior selection of female adults of T. flavus to different concentrations of the seven substances was determined by Y-type olfactometer in the laboratory. The results showed that 10.0 μg / mL α-pinene, 10.0 μg / mL caryophyllene, 10.0 μg / mL DL-limonene, 1.0 μg / mL D-limonene, and 1.0 μg / mL β-pinene had significant attractive effect on female adults of T. flavus. Further, the active compounds were combined with blue sticky boards, and the results showed that the five substances significantly increased the trapping amount of T. flavus on the blue sticky boards.

[0031] According to the results of indoor and outdoor experiments, five single compounds (α-pinene, caryophyllene, DL-limonene, D-limonene, and β-pinene) were selected as the single active compounds with significant attractive effect on T. flavus. After determining the optimal concentration parameters of the five single compounds (10.0 μg / mL α-pinene, 10.0 μg / mL caryophyllene, 10.0 μg / mL DL-limonene, 1.0 μg / mL D-limonene, and 1.0 μg / mL β-pinene), the test system was constructed according to the principle of “free combination”, including two combinations, three combinations, four combinations, and five combinations. The single compounds were prepared according to the indoor and outdoor test results, and 10.0 μg / mL α-pinene (A), 10.0 μg / mL caryophyllene (B), 10.0 μg / mL DL-limonene (C), 1.0 μg / mL D-limonene (D), and 1.0 μg / mL β-pinene (E) were prepared according to different combinations and proportions.

[0032] The behavior selection of female adults of T. flavus to the above combinations was determined by Y-type olfactometer, and the determination method was the same as that in 1.3. The active combinations with higher indoor attractive activity were selected and statistically analyzed. The indoor behavior selection results of female adults of T. flavus were as follows: Figure 4As shown in Table 1, the highest selection rate of two-by-two combination of the five compounds was 55.0%, and the attraction effect did not reach a significant level; the three-by-three combination, five combinations were BCD (volume ratio 1:1:01) with a selection rate of 55.0%, ADE (volume ratio 1:0.1:0.1) with a selection rate of 51.7%, ACD (volume ratio 1:1:0.1) with a selection rate of 58.3%, ABE (volume ratio 1:1:0.1) with a selection rate of 56.7%, and ABD (volume ratio: 1:1:0.1) with a selection rate of 58.3%, and the attraction effect reached a significant level; the highest selection rate of four-by-four combination was 61.8%, and five combinations were BCDE (volume ratio 1:1:0.1:0.1) with a selection rate of 68.3%, ABCD (volume ratio 1:1:1:0.1) with a selection rate of 61.7%, ABCE (volume ratio 1:1:1:0.1) with a selection rate of 65.0%, ACDE (volume ratio 1:1:0.1:0.1) with a selection rate of 58.3%, and ABDE (volume ratio 1:1:0.1:0.1) with a selection rate of 61.7%, and the attraction effect reached a very significant level; the combination of all single agents (volume ratio of ABCDE was 1:1:1:0.1:0.1) had a selection rate of 70.0%, and the attraction effect reached a very significant level.

[0033] Further, the combinations with significant attraction activity to female adult yellow thorax thrips (four-by-four combination and all compound combination) were selected, and these combinations were combined with blue armyworm plates. The test group (highly active combination + blue armyworm plate) and the control group (blank blue armyworm plate) were set in the banana garden during the peak period of yellow thorax thrips occurrence. The actual trapping efficiency of the trapping system on field yellow thorax thrips and the application potential were systematically evaluated through continuous 5-day number statistics. The results are shown in Table 2. Figure 5 As shown in Table 2, the number of female adult yellow thorax thrips trapped by the five all-compound combinations (ABCDE) was 59.4, 65.8, 66.6, 60.6, 65.6, and 71.2 for BCDE (volume ratio 1:1:0.1:0.1), ABCD (volume ratio 1:1:1:0.1), ABCE (volume ratio 1:1:1:0.1), ACDE (volume ratio 1:1:0.1:0.1), ABDE (volume ratio 1:1:0.1:0.1), and ABCDE (volume ratio 1:1:1:0.1:0.1), respectively, and the number of blue plate treatment was 12.4, and the number of n-hexane and blue plate combination was 14.4. The trapping amount of the combination of attractants was significantly higher than that of the combination of solvent and blue armyworm plate and the blank control (only blue plate); among different attractant treatments, the trapping amount of the combination of all single agents was significantly higher than that of the four-by-four combination ACDE and BCDE, but there was no significant difference between the combination of ABCD, ABCE, and ABDE.

[0034] The indoor and outdoor experiments prove that the attracting composition has significant attracting activity on the yellow stern thrips. The plant source attractant and method provided by the application can be applied to monitoring the population dynamics of the yellow stern thrips in a banana garden and guiding the prevention and treatment of the yellow stern thrips by using the trapping control technology in the field. The attractant provided by the application is derived from plants, simple to prepare, friendly to the environment, non-toxic to humans and animals, and has a good application prospect.

Claims

1. A plant-derived attractant for thrips hawaiiensis in a banana plantation, characterized by: The attractant is any one of alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene or a combination thereof, the concentration of alpha-pinene is 10.0 μg / mL, caryophyllene is 10.0 μg / mL, DL-limonene is 10.0 μg / mL, D-limonene is 1.0 μg / mL, beta-pinene is 1.0 μg / mL.

2. The plant-derived attractant for T. flavotesticulata in a banana plantation according to claim 1, characterized by: The composition is any one of the following combinations: alpha-pinene, caryophyllene and D-limonene, or alpha-pinene, caryophyllene and beta-pinene, or alpha-pinene, DL-limonene and D-limonene, or alpha-pinene, D-limonene, beta-pinene, or caryophyllene, DL-limonene, D-limonene, or alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene in any four or five combinations.

3. The plant-derived attractant for yellow-breasted thrips in banana plantations as described in claim 1, characterized in that: The caryophyllene, DL-limonene, beta-pinene, D-limonene are in a volume ratio of 1:1:0.1:0.1, or the alpha-pinene, caryophyllene, DL-limonene, D-limonene are in a volume ratio of 1:1:1:0.1, or the alpha-pinene, caryophyllene, DL-limonene, beta-pinene are in a volume ratio of 1:1:1:0.1, or the alpha-pinene, DL-limonene, D-limonene, beta-pinene are in a volume ratio of 1:1:0.1:0.1, or the alpha-pinene, caryophyllene, D-limonene, beta-pinene are in a volume ratio of 1:1:0.1:0.

1.

4. The plant-derived attractant for thrips hawaiiensis in a banana plantation according to claim 3, characterized by: The composition is alpha-pinene, caryophyllene, DL-limonene, beta-pinene, D-limonene in a volume ratio of 1:1:1:0.1:0.

1.

5. Use of the plant-derived attractant of the yellow chested stink bug in a banana plantation according to any one of claims 1-4 in the preparation of a product for monitoring the occurrence of the yellow chested stink bug in a banana plantation or for physical control measures.

6. A method for monitoring or physically controlling the yellow chested stink bug in a banana plantation, comprising the following steps: using the plant-derived attractant according to any one of claims 1-4 or using the plant-derived attractant according to any one of claims 1-4 in combination with a trapping device to trap the yellow chested stink bug in a banana plantation.

7. The method of claim 6, wherein, The trapping device is a blue sticky board.

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