Application of Brassica napus gene BnaDOF31 in breeding of Brassica napus resistant to sclerotinia stem rot

By identifying and utilizing the Brassica napus gene BnaDOF31, and editing rapeseed genes using the CRISPR/Cas9 system, the deficiency in regulating resistance to Sclerotinia stem rot in rapeseed was resolved, resulting in enhanced disease resistance for high and stable yields.

CN121344072BActive Publication Date: 2026-07-24OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511831307.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-12-07
Publication Date
2026-07-24
Estimated Expiration
2045-12-07

AI Technical Summary

Technical Problem

In the current technology, the regulatory network of rapeseed resistance to sclerotinia stem rot is not fully understood, and there is a lack of effective gene regulation methods to improve rapeseed resistance to sclerotinia stem rot.

Method used

By identifying and utilizing the Brassica napus gene BnaDOF31, the expression of the BnaDOF31 gene was knocked out or silenced using the CRISPR/Cas9 system to regulate the resistance of Brassica napus to sclerotinia stem rot. Gene editing was then achieved by introducing recombinant vectors or recombinant microorganisms into the Brassica napus gene.

Benefits of technology

This study enhanced rapeseed's resistance to sclerotinia stem rot, ensured high and stable yields, and enriched research on molecular networks of plant disease resistance.

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Abstract

The application belongs to the field of oilseed rape breeding, and specifically discloses application of a Brassica napus gene BnaDOF31 in breeding of Brassica napus resistant to sclerotinia stem rot, wherein the protein encoded by the BnaDOF31 gene is shown as SEQ ID NO. 2; the BnaDOF31 gene of Brassica napus is identified for the first time to be capable of regulating resistance of oilseed rape to sclerotinia stem rot, and the application enriches a molecular network for regulating resistance of plants to sclerotinia stem rot and plays an important role in research on a molecular mechanism for formation of disease resistance of plants. Overexpression of the BnaDOF31 gene disclosed by the application significantly reduces resistance of oilseed rape to sclerotinia stem rot, and a plaque area of a knock-out strain shows a downward trend; the application provides a new negative regulation site for resistance of oilseed rape to sclerotinia stem rot, provides a new target for genetic improvement of disease-resistant oilseed rape, and has important significance for guaranteeing high yield and stable yield of oilseed rape.
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