Composition for enhancing intestinal tolerance and application thereof

Abstract

The invention belongs to the technical field of clinical nutrition, and particularly relates to a composition for enhancing intestinal tolerance and application thereof. The composition is composed of 20%-85% of rice flour and 12%-30% of yeast protein, and the osmotic pressure is smaller than or equal to 300 mOsm / kg. The rice flour is gelatinized rice flour with more than or equal to 70% of amylopectin, and has a slow-release energy supply characteristic; the yeast protein is slowly digestible protein and is rich in beta-glucan. According to the composition, through a'low osmotic pressure-low antigen-barrier repair 'three-axis synergistic mechanism, the diarrhea occurrence rate is remarkably reduced, and the composition is suitable for people with short bowel syndrome, senile diarrhea, postoperative intestinal hypofunction and the like.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of clinical nutrition, and particularly relates to a composition for enhancing intestinal tolerance and application thereof. BACKGROUND

[0002] At present, there are about 15 million cases of patients requiring long-term bed rest or enteral nutrition support per year. The reasons for long-term bed rest or enteral nutrition support of these patients include sequelae after cerebral apoplexy, postoperative ICU, senile debilitation, short bowel syndrome, etc. Due to weakened intestinal peristalsis, decreased secretion of digestive juice, and imbalance of intestinal flora, the incidence of diarrhea of this group of people is as high as 42% to 68%. Diarrhea not only endangers patients but also brings a chain of panic to family members and medical staff. Once severe diarrhea occurs, it can lead to water-electrolyte disturbance, hypovolemic shock, and increase the mortality rate of ICU by 2.3 times; chronic persistent watery stool is prone to cause protein-energy malnutrition in patients, and the incidence of complications such as pressure sores and pneumonia also increases simultaneously; in addition, frequent replacement of nursing pads and nutritional preparations brings psychological and economic double burdens to patients and family members, and also makes nursing staff "fear diarrhea".

[0003] The mainstream enteral nutritional preparation adopts whey protein, casein protein or soybean protein as the main nitrogen source. The osmotic pressure is generally 350-450 mOsm·kg -1 , which is much higher than the intestinal tolerance threshold (≤ 300 mOsm·kg -1 ), and is easy to trigger osmotic diarrhea; in addition, the mainstream nitrogen source often carries strong antigens such as beta-lactoglobulin, alpha-casein and soybean protein, and the residual lactose in milk-derived nitrogen can also bring double damage to patients with secondary lactose intolerance.

[0004] The existing enteral nutritional preparation is oriented to "nitrogen supplementation", however, the intestinal epithelium is not a simple absorption pipeline, but a highly dynamic and continuously "maintained" living barrier. Only the content of high-quality protein such as whey protein or soybean protein is concerned, and the repair of intestinal epithelial tight junction is ignored, which is easy to form a negative cycle of "nitrogen supplementation-nitrogen leakage". SUMMARY

[0005] In view of the defects of high osmotic pressure of the existing enteral nutritional preparation, large antigen load of the mainstream nitrogen source and inability to simultaneously repair the intestinal barrier, the present application provides a diarrhea-friendly nutritional composition, which can reduce the incidence of diarrhea, improve the stool character and repair the intestinal barrier. The composition is composed of rice powder and yeast protein.

[0006] In order to achieve the above purpose, the present application provides a composition for enhancing intestinal tolerance, which is composed of the following components in weight percentage: 20% to 85% of rice powder and 12% to 30% of yeast protein; The rice powder is a gelatinized rice powder with a content of amylopectin ≥ 70%, and the content of β-glucan in the yeast protein is ≥ 2%. The osmotic pressure of the composition is ≤ 300 mOsm / kg.

[0007] In the present application, the composition further comprises one or more of the following auxiliary materials: carbohydrates 0-55%, oils and fats 0-30%, complex vitamins 0-2%, and complex minerals 0-10%.

[0008] In the present application, the carbohydrates include one or more of malt dextrin, cassava dextrin, high-branching cyclic dextrin, isomaltulose, and starch. The oils and fats include one or more of medium-chain triglycerides, DHA oil, ARA oil, soybean oil, rapeseed oil, olive oil, corn oil, sunflower oil, coconut oil, and flaxseed oil. The complex vitamins include one or more of vitamins A, D, E, K, B1, B2, B6, B 12 , C, nicotinamide, folic acid, pantothenic acid, biotin, and taurine. The complex minerals include one or more of sodium, potassium, calcium, magnesium, iron, zinc, copper, manganese, and selenium.

[0009] In the present application, the composition further comprises one or more of the following functional ingredients: glutamine, arginine, probiotics, yam, gordon euryale, and lotus seed.

[0010] In the present application, the dosage form of the composition includes powder, suspension, or ready-to-drink emulsion.

[0011] In the present application, the method for using the composition is as follows: The composition is reconstituted in water at 40-100°C, and the osmotic pressure of the reconstituted composition is maintained at 150-300 mOsm / kg.

[0012] The composition for enhancing intestinal tolerance provided in the present application does not contain milk-derived or bean-derived antigenic proteins.

[0013] The present application also provides the use of the composition described in any one of the above in the preparation of a nutritional agent for improving intestinal tolerance, reducing the incidence of diarrhea, and repairing intestinal barrier function.

[0014] In the present application, the population for which the nutritional agent is suitable includes: patients with short bowel syndrome, inflammatory bowel disease, radiation enteritis, low digestive capacity in the elderly, and postoperative intestinal hypofunction.

[0015] Compared with existing technologies, this invention is the first to utilize a simple low-antigen composition—rice flour and yeast protein containing β-glucan. The composition provided by this invention promotes intestinal barrier repair while eliminating milk / soybean-derived antigen stimulation at the source, effectively reducing the risk of allergies; its osmotic pressure is ≤300 mOsm / kg, below the intestinal tolerance threshold; and it can effectively improve diarrhea in patients. In terms of usage, this composition can be prepared with water at temperatures ranging from 40 to 100°C, fully meeting the various operational conditions of clinical nutrition departments. Detailed Implementation

[0016] For reagents or instruments used in the following text where specific technical or conditional specifications are not given, standard experimental conditions shall apply. If no reagent company instructions are explicitly provided, the conditions recommended in those instructions shall be followed. Reagents or instruments whose manufacturers are not specified are all commercially available standard products.

[0017] All participants in this study were fully informed of the study's purpose, procedures, potential benefits, and risks at the time of recruitment, and their right to full informed consent was ensured. All data from this study were anonymized and used solely for scientific statistical analysis.

[0018] Example 1 The total weight is 100 parts by mass, including 15 parts yeast protein, 45 parts rice flour, and 0.6 parts of a complex vitamin (by mass: Vitamin A 1.1 parts, Vitamin D 0.1 parts, Vitamin E 7.1 parts, Vitamin B1 0.3 parts, Vitamin B2 2.6 parts, Vitamin B6 2.8 ... 12 The mixture contains 0.9 parts of sodium chloride, 33.3 parts of vitamin C, 3.2 parts of nicotinamide, 0.5 parts of folic acid, 1.2 parts of pantothenic acid, and 10 parts of taurine; 6.0 parts of complex minerals (by weight: 3 parts of sodium chloride, 10 parts of potassium citrate, 10 parts of magnesium carbonate, 14 parts of calcium carbonate, 1.8 parts of sodium iron EDTA, and 1.2 parts of zinc gluconate); and 33.4 parts of maltodextrin. All the above substances are mixed thoroughly. Take 50g and add approximately 170ml of 50℃ warm water to prepare an isocaloric solution. The measured osmotic pressure is approximately 186 mOsm / kg.

[0019] Example 2 The total weight is 100 parts, including 20 parts yeast protein, 40 parts rice flour, 20 parts fat (added in the form of microencapsulated powdered oil), and 0.6 parts of compound vitamins (by weight, vitamin A 1.1 parts, vitamin D 0.1 parts, vitamin E 7.1 parts, vitamin B1 0.3 parts, vitamin B2 2.6 parts, vitamin B6 2.8 parts, and vitamin B...). 120.9 parts of vitamin C, 33.3 parts of nicotinamide, 3.2 parts of folic acid, 0.5 parts of pantothenic acid, 1.2 parts of taurine, 6.0 parts of complex minerals (by weight: 3 parts of sodium chloride, 10 parts of potassium citrate, 10 parts of magnesium carbonate, 14 parts of calcium carbonate, 1.8 parts of sodium iron EDTA, 1.2 parts of zinc gluconate), and 13.4 parts of maltodextrin. Mix all the above substances evenly, take 50g, add about 180ml of 50℃ warm water to make an isocaloric solution, and the osmotic pressure was measured to be about 225 mOsm / kg.

[0020] Example 3 The total weight is 100 parts, including 25 parts yeast protein, 30 parts rice flour, 20 parts fat powder (in microencapsulated form), and 0.6 parts of compound vitamins (based on weight: Vitamin A 1.1 parts, Vitamin D 0.1 parts, Vitamin E 7.1 parts, Vitamin B1 0.3 parts, Vitamin B2 2.6 parts, Vitamin B6 2.8 parts, Vitamin B...). 12 The following ingredients were mixed evenly: 0.9 parts of Vitamin C, 33.3 parts of Vitamin C, 3.2 parts of Nicotinamide, 0.5 parts of Folic Acid, 1.2 parts of Pantothenic Acid, and 10 parts of Taurine; 6.0 parts of Complex Minerals (by weight: 3 parts of Sodium Chloride, 10 parts of Potassium Citrate, 10 parts of Magnesium Carbonate, 14 parts of Calcium Carbonate, 1.8 parts of Sodium Iron EDTA, and 1.2 parts of Zinc Gluconate); 4.8 parts of Dietary Fiber (1.6 parts of Fructooligosaccharides, 1.6 parts of Inulin, and 1.6 parts of Galactomannan); 2.0 parts of Yam; 1.0 part of Euryale Ferox; 0.6 parts of Lotus Seed; and 10 parts of Maltodextrin. 50g of the mixture was added to approximately 180ml of 50℃ warm water to prepare an isocaloric solution. The measured osmotic pressure was approximately 266 mOsm / kg.

[0021] Example 1. Subjects A total of 24 elderly people (aged 65-85) with poor intestinal function were recruited from nursing homes and randomly divided into two groups. 2. Test Sample The two groups of samples had the same energy ratio of carbohydrates, protein, and fat, and the same source and content of fat and vitamins and minerals, but different sources of carbohydrates and protein. The specific sample composition and grouping are as follows.

[0022] Control group (n=12): took sample A (protein 20 parts (from soy and whey concentrate), rice flour 15 parts, maltodextrin 40.4 parts, fat powder 20 parts, multivitamins 0.6 parts, multimineral complex 6.0 parts). Test group (n=12): Take sample B (20 parts yeast protein, 40 parts rice flour, 15.4 parts maltodextrin, 20 parts fat powder, 0.6 parts compound vitamins, and 6.0 parts compound minerals, i.e. the product provided in Example 2).

[0023] 3. Experimental design Adaptation period (7 days): record baseline data of volunteers (defecation frequency, stool characteristics); Intervention period (28 days): measure and record the basic vital signs, defecation frequency and stool characteristics of volunteers every day; 4. Detection index and data collection Diarrhea incidence and stool characteristics; diarrhea event record uses FDA standard: ≥3 times of Bristol 6-7 type within 24 hours and each time interval ≥1 hour, count 1 diarrhea number, the calculation method of diarrhea incidence is total number of diarrhea / intervention days * number of intervention persons. Bristol stool form scale is shown in Table 1.

[0024] Table 1 Bristol stool form scale

[0025] 5. The results are as follows: (1) Diarrhea incidence: after 4 weeks of intervention, the diarrhea incidence of the comparison group is 15.8%, and the diarrhea incidence of the test group is 0.9%; it is shown that eating the composition containing rice flour and yeast protein is more conducive to improving intestinal tolerance than eating the conventional milk source / bean source rice flour combination.

[0026] (2) Stool characteristics: after 28 days of intervention, the proportion of Bristol stool type 4 (ideal normal stool) is counted, and the results are that the proportion of type 4 stool in the test group increases from 33% to 58%, while the proportion of type 4 stool in the comparison group does not change significantly before and after intervention; no adverse events such as abdominal distension, nausea, vomiting, etc. are observed in the comparison group and the test group during the experiment.

[0027] The above is only the preferred embodiment of the present application, it should be noted that for the inventors of the present application, on the premise of not departing from the principles of the present application, a number of improvements and refinements can also be made, which should be considered as the protection scope of the present application.

Claims

1. A composition for enhancing intestinal tolerance, characterized in that, The composition is composed of the following components by weight percentage: Rice powder 20%-85%, yeast protein 12%-30%; The rice powder is gelatinized rice powder with amylopectin content ≥70%, and the yeast protein has β-glucan content ≥2%; The osmotic pressure of the composition is ≤300 mOsm / kg.

2. The composition of claim 1, wherein, The composition further comprises one or more of the following auxiliary materials: Carbohydrate 0-55%, oil 0-30%, complex vitamin 0-2%, and complex mineral 0-10%.

3. The composition of claim 1, wherein, The carbohydrate includes one or more of malt dextrin, cassava dextrin, high-amylopectin cyclic dextrin, isomaltulose, and starch; The oil includes one or more of medium-chain triglyceride, DHA oil, ARA oil, soybean oil, rapeseed oil, olive oil, corn oil, sunflower oil, coconut oil, and flaxseed oil; The complex vitamin includes one or more of vitamin A, D, E, K, Bl, B2, B6, B 12 , C, nicotinamide, folic acid, pantothenic acid, biotin, taurine The complex mineral includes one or more of sodium, potassium, calcium, magnesium, iron, zinc, copper, manganese, and selenium.

4. The composition of claim 1, wherein, The composition further comprises one or more of the following functional ingredients: glutamine, arginine, probiotics, yam, gualou, and lotus seed.

5. The composition of claim 1, wherein The dosage form of the composition includes powder, suspension, or ready-to-drink emulsion.

6. The composition of claim 1, wherein, The use method of the composition is: Reconstitute in water at 40-100°C, and keep the osmotic pressure of the reconstituted product at 150-300 mOsm / kg.

7. Use of the composition according to any one of claims 1-6 in the preparation of a nutritional agent for improving intestinal tolerance, reducing the incidence of diarrhea, and repairing intestinal barrier function.

8. Use according to claim 7, characterized in that, The population for which the nutritional agent is suitable includes: Patients with short bowel syndrome, inflammatory bowel disease, radiation enteritis, low digestive power in the elderly, and postoperative intestinal hypofunction.

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