Method and system for preparing bone biological structure of two-way bionic of force biology special trabecular zone of tendon / ligament bone junction
By combining the morphological and mechanical parameters of the trabecular bone at the tendon/ligament-bone junction, a dopamine-modified biomimetic bone structure was prepared using 3D printing technology. This solved the mechanical load requirements of the tendon/ligament-bone junction area in existing technologies, achieving better biocompatibility and mechanical stability, and reducing the surgical failure rate.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- DALIAN MEDICAL UNIVERSITY
- Filing Date
- 2025-12-03
- Publication Date
- 2026-05-19
AI Technical Summary
Existing biomimetic material fabrication technologies cannot meet the long-term mechanical load requirements of the tendon/ligament-bone junction area, leading to surgical reconstruction failure and a high revision rate. Furthermore, existing surgical methods cannot accurately locate the bone tunnel, affecting the biocompatibility and mechanical stability of the implant and bone tissue.
By combining the morphological and mechanical parameters of the trabecular bone at the tendon/ligament bone junction, a biomimetic bone biostructure was prepared using 3D printing technology. The biocompatibility was improved by dopamine modification treatment, and a bone biostructure conforming to the tendon/ligament bone junction was prepared.
This approach achieves bidirectional biomechanical and biological biomimicry at the tendon/ligament-bone junction, improving the biocompatibility and mechanical stability of the implant with bone tissue and reducing the risk of surgical failure.
Smart Images
Figure CN121401512B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of bone biomaterial preparation technology, and more specifically to a method and system for preparing a bone biostructure with bidirectional biomechanical and biological mimicry in a special trabecular region at the tendon / ligament bone junction. Background Technology
[0002] The tendon / ligament-bone junction is a special transitional area connecting tendons, ligaments, and bone tissue. It is composed of various histological structures, including tendons, fibrocartilage, calcified fibrocartilage, and bone tissue. Its core function is to achieve efficient force transmission and cushioning, making it a key structure for maintaining the stability of the musculoskeletal system. With the development of biomimetic design concepts in biomaterials, biomimetic material design based on the histological features of this region has become an emerging technological direction. Existing technologies mainly combine organic solvent methods with casting methods, employing layer-by-layer casting or layer-by-layer co-pressing processes to composite organic biocompatible polymers with inorganic bioactive polymers to prepare biomimetic materials that simulate the histological structure of this region.
[0003] However, the tendon / ligament-bone junction area is subjected to the traction of tendons or ligaments over a long period, resulting in the formation of specific morphological and distributional characteristics of the underlying bone structure that adapt to mechanical loads. Existing biomimetic material fabrication techniques can only achieve morphological similarity to the anatomical structure of this area, but neglect the actual biomechanical performance requirements formed under long-term mechanical loads. This leads to a disconnect between the fabricated materials and the actual application requirements for mechanical transmission and load tolerance during surgery, making it difficult to meet the functional requirements of clinical repair.
[0004] Currently, surgical reconstruction remains the primary clinical treatment for tendon / ligament-bone junction injuries. However, existing surgical methods have significant drawbacks, leading to a high revision rate and often failing to meet patient expectations in the long term. Specifically, current procedures require creating a bone tunnel within the bone tissue before inserting fixation screws to secure the graft. During this process, the bone tunnel is completely filled with screws, causing the screws to replace the original biomechanical properties of the tunnel. Furthermore, current techniques lack effective methods for precisely locating the bone tunnel, making it impossible to ensure that the screw path matches the anatomical characteristics of the specific trabecular bone region at the tendon / ligament-bone junction. Additionally, the elastic modulus of the screws differs significantly from that of the bone tissue, drastically altering the original mechanical transmission path and load distribution patterns after implantation. This ultimately results in poor graft-bone integration, mechanical instability, and ultimately, surgical failure.
[0005] Therefore, how to provide a method and system for preparing bone biological structures that are similar in mechanical structure and material properties to those at the tendon / ligament-bone junction is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention
[0006] In view of this, the present invention provides a method and system for preparing a bone biostructure with bidirectional biomechanical and biological mimicry in the special trabecular region of the tendon / ligament bone junction. By combining the construction information of the trabecular bone at the tendon / ligament bone junction with 3D printing technology, the structure and materials of the implant are synergistically biomimetic during the reconstruction of the tendon / ligament bone junction, providing important technical support for the development of bone biostructures with better biocompatibility at the tendon / ligament bone junction.
[0007] To achieve the above objectives, the present invention adopts the following technical solution:
[0008] On one hand, this invention provides a method for preparing a bidirectional biomimetic bone structure in the special trabecular region at the tendon / ligament-bone junction, comprising:
[0009] Morphological information of trabeculae was extracted from human specimen sections of the target region.
[0010] Obtain biomechanical parameters of the tendon / ligament-bone junction through anatomical analysis of human specimens;
[0011] Biomimetic structures are determined based on the morphological information of trabeculae;
[0012] Biomaterials with biomimetic structures were prepared based on the mechanical parameters of the tendon / ligament-bone junction.
[0013] Biomimetic bone biostructures are prepared based on biomimetic structures and biomaterials;
[0014] The surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure.
[0015] Preferably, morphological information of bone trabeculae is extracted from human specimen slices of the target region, including:
[0016] Multiple tomographic sections of the target area of the human specimen were prepared using polymer materials and photographed.
[0017] Using programming software, slice images with the largest attachment area between soft tissue and bone were selected from the tomographic sections according to the course of the soft tissue at the tendon / ligament-bone junction and the way it is attached to the bone, and then digitally processed.
[0018] Quantitative analysis of trabecular bone in the digitized slice images was performed to obtain morphological information of trabecular bone in the target region.
[0019] Preferably, the morphological information includes the orientation, number, porosity, and anisotropy of the trabeculae.
[0020] Preferably, the mechanical parameters of the tendon / ligament-bone junction are obtained through anatomical analysis based on human specimens, including:
[0021] Only the bone tissue at the tendon / ligament attachment site and the area containing reactive trabeculae within the bone is preserved;
[0022] Micro-CT scans were performed on the acquired bone tissue to obtain imaging data of bone emboli;
[0023] A three-dimensional model is generated based on the imaging data of the bone thrombus. Mechanical tests are then performed on the three-dimensional model and the solid bone thrombus to obtain the mechanical parameters at the tendon / ligament-bone junction.
[0024] Preferably, the preparation of biomimetic bone biostructures based on biomimetic structures and biomaterials includes:
[0025] The biomimetic structure, determined by morphological data of trabeculae, is fabricated using biomaterials based on mechanical parameters at the tendon / ligament-bone junction. The biomimetic structure is then 3D printed at a preset temperature to generate a solid implant, i.e., a biomimetic bone biostructure.
[0026] Preferably, the surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure, including:
[0027] The solid implant was soaked in a dopamine aqueous solution for 12 hours with continuous stirring, so that the dopamine in the dopamine aqueous solution was converted into polydopamine and adsorbed onto the surface of the solid implant.
[0028] Hydroxyapatite was soaked in a dopamine aqueous solution for 12 hours with continuous stirring to generate polydopamine-modified hydroxyapatite.
[0029] The modified hydroxyapatite and the modified solid implant were soaked in a dopamine aqueous solution for 12 hours with continuous stirring and in a dark environment. This process allowed the hydroxyapatite to be adsorbed onto the surface of the solid implant, resulting in a biomimetic bone structure that is both mechanical and biologically similar at the tendon / ligament-bone junction.
[0030] On the other hand, the present invention provides a system for fabricating bone biostructures in a special trabecular region at the tendon / ligament-bone junction that is biomechanically and biologically incorporating both methods, comprising:
[0031] The morphological parameter extraction unit is used to extract morphological information of bone trabeculae from human specimen slices based on the target region.
[0032] The mechanical parameter extraction unit is used to obtain mechanical parameters of the tendon / ligament-bone junction through anatomical analysis based on human specimens.
[0033] A biomimetic structural design unit is used to determine the biomimetic structure of the implant based on the morphological information of the trabeculae;
[0034] A bone biomaterial determination unit is used to determine the biomaterials for preparing biomimetic bone biostructures based on the mechanical parameters of the tendon / ligament bone junction.
[0035] A biomimetic bone biostructure fabrication unit is used to fabricate biomimetic bone biostructures based on biomimetic structures and biomaterials.
[0036] Modification units are used to modify the surface of biomimetic bone structures to obtain the final biomimetic bone structure.
[0037] As can be seen from the above technical solution, compared with the prior art, this invention discloses a method and system for preparing a biomimetic bone structure in a special trabecular region at the tendon / ligament-bone junction using both mechanical and biological methods. Based on polymer material embedding technology, such as P45 tomographic plasticization, morphometric anatomical parameters of the trabecular bone at the tendon / ligament-bone junction are extracted. By locating the precise position of the bone tunnel using the morphological parameters of the trabecular bone, and simultaneously acquiring biomimetic screw data, an anatomical foundation is laid for the preparation of biomimetic bone materials at the tendon / ligament-bone junction. Furthermore, this invention combines the structural information of the trabecular bone at the tendon / ligament-bone junction with 3D printing technology to achieve synergistic biomimicry of the implant's structure and materials during tendon / ligament-bone junction reconstruction, providing important technical support for the development of biocompatible bone structures at the tendon / ligament-bone junction. Attached Figure Description
[0038] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.
[0039] Figure 1 This is a schematic diagram of the process provided by the present invention.
[0040] Figure 2 This is to extract morphological information of trabecular bone and bone tissue at the ligament-bone junction.
[0041] Figure 3 To obtain the biomechanical information of the bone tissue at the ligament-bone junction and to design the fixation screws.
[0042] Figure 4 A flowchart for constructing a cell growth microenvironment.
[0043] Figure 5 This is a physical image of a fixation screw that uses both mechanical and biological biomimicry in anterior cruciate ligament reconstruction.
[0044] Figure 6 The system structure diagram provided for this invention. Detailed Implementation
[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0046] This invention discloses a method for preparing a bidirectional biomimetic bone structure in the special trabecular region at the tendon / ligament-bone junction, which is based on both biomechanics and biology. Figure 1 As shown, it includes:
[0047] Morphological information of trabeculae was extracted from human specimen sections of the target region.
[0048] Obtain biomechanical parameters of the tendon / ligament-bone junction through anatomical analysis of human specimens;
[0049] Based on the morphological information of the trabeculae, the biomimetic structure, namely the pore structure of the implant, is determined so that the pore structure of the implanted screw fully conforms to the original structural characteristics of the bone tunnel.
[0050] Biomaterials for preparing biomimetic bone biostructures based on mechanical parameters at the tendon / ligament-bone junction;
[0051] Biomimetic bone biostructures are prepared based on the porous structure of implants and biomaterials.
[0052] The surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure.
[0053] Specifically, morphological information of bone trabeculae is extracted from human specimen slices of the target region, including:
[0054] Multiple tomographic sections of the target area of the human specimen were prepared using polymer materials and photographed.
[0055] Programming software was used to select slice images with the largest attachment area between soft tissue and bone at the tendon / ligament-bone junction based on the course of the soft tissue and its connection with bone. The slice images were then digitally processed, and the soft tissue in the images was removed in the software, leaving only the bone tissue, in order to reduce the interference of soft tissue information.
[0056] Quantitative analysis of trabecular bone in digitized slice images was performed to obtain morphological information of trabecular bone in the target region. Specifically, the adaptive trabecular bone region at the tendon / ligament-bone junction was selected as the target region, and morphological data of trabecular bone within this region were quantitatively analyzed.
[0057] Furthermore, the morphological information includes the orientation, number, porosity, and anisotropy of the trabeculae.
[0058] Furthermore, anatomical analysis based on human specimens is used to obtain biomechanical parameters at the tendon / ligament-bone junction, including:
[0059] Before dissection, the specimens are registered, measured, and photographed. A detailed dissection is performed, removing excess soft and bone tissue from the human specimen, preserving only the tendon / ligament attachments to the bone surface and the area containing reactive trabecular bone (i.e., the area from which trabecular morphology information is extracted).
[0060] Micro-CT scans were performed on the acquired bone tissue to obtain imaging data of bone emboli;
[0061] A three-dimensional model is generated based on the imaging data of the bone thrombus. Mechanical tests are then performed on the three-dimensional model and the solid bone thrombus to obtain the mechanical parameters at the tendon / ligament-bone junction.
[0062] Furthermore, biomimetic bone biostructures are fabricated based on the porous structure of implants and biomaterials, including:
[0063] The porous structure of the implant is imported into the 3D printing program. Biomaterials for preparing the biomimetic structure are determined based on the mechanical parameters of the tendon / ligament-bone junction. The biomimetic structure is then 3D printed at a preset temperature to generate a solid implant, i.e., a biomimetic bone biostructure.
[0064] Preferably, the surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure, including:
[0065] The solid screw was immersed in a 50 ml solution of tris(hydroxymethyl)aminomethane hydrochloride containing dopamine (2 mg / ml) for 12 hours, so that the dopamine in the solution would generate polydopamine and be adsorbed onto the surface of the solid screw.
[0066] Hydroxyapatite was soaked in a 50 ml solution of tris(hydroxymethyl)aminomethane hydrochloride containing dopamine (2 mg / ml) for 12 hours to generate polydopamine-modified hydroxyapatite.
[0067] The modified hydroxyapatite and the modified solid screw were immersed in a 50 ml solution of tris(hydroxymethyl)aminomethane hydrochloride for 12 hours with continuous stirring and in a dark environment. Finally, using polydopamine as a medium, the hydroxyapatite was adsorbed onto the implant surface, resulting in a biomimetic bone structure at the tendon / ligament-bone junction that is both mechano-biologically similar.
[0068] Furthermore, this embodiment will be illustrated using the preparation of a biomimetic bone biomaterial with both mechanical and biological characteristics at the anterior cruciate ligament attachment site as an example:
[0069] Anterior cruciate ligament (ACL) injury is one of the most common and destructive knee injuries. To date, anterior cruciate ligament reconstruction (ACLR) remains the gold standard for treating ACL injuries. Literature reports a failure rate of 10%–40%, primarily manifesting as short-term postoperative knee instability and rotational laxity, and long-term progression to meniscus and cartilage damage, as well as knee osteoarthritis. Simply put, ACLR involves reconstructing a graft on the femur and tibia to replace the ACL. The procedure involves two key steps: locating the bone tunnel and fixing the graft within it. To reproduce the function and stability of the original ACL and improve the success rate, researchers have conducted detailed anatomical studies of the ACL attachment site and discovered that the interface is a complex transitional structure between collagen fibers and bone tissue, composed of ligaments, fibrocartilage, and bone. This transitional structure can transfer and buffer stress, reducing the tensile load at the ligament-bone junction. Based on this, researchers have designed various biomimetic ACL reconstruction strategies, striving to reproduce this transitional structure after ACLR, restore the original biomechanical properties within the bone tunnel, and improve the success rate of ACLR. However, regardless of the biomimetic strategy employed, the surgery still faces a high risk of revision. The main reason for surgical failure is the inability to restore the original mechanical properties of the bone tunnel, primarily involving inaccurate bone tunnel positioning and poor biocompatibility of the fixation screws. To systematically address this problem, the present invention is as follows:
[0070] Step 1: Extract morphological information of the trabecular bone at the anterior cruciate ligament attachment site:
[0071] Human knee joint specimens treated with formalin were selected, and imaging studies confirmed the absence of developmental deformities, fractures, and other lesions. The selected human knee joint specimens underwent a series of polymer embedding steps, including freezing, sectioning, bleaching, dehydration, degreasing, vacuum impregnation, and curing, to finally obtain human knee joint specimen sections. The sections were then photographed. Figure 2 As shown, sagittal sections were taken at the tibial end of the anterior cruciate ligament (ACL) at its attachment to the tibia and femur, according to the ACL's course and its connection with the femur. For each specimen, one photograph was taken along the long axis of the ACL at the plane of the intercondylar fossa, showing the ACL's maximum attachment to the tibia. Coronal sections were taken at the femoral end of the ACL, also showing one photograph of the ACL's maximum attachment to the femur. The tissues in the sections were intact and without defects. The distribution range of adaptive trabeculae within the bone, with the ACL attachment cortex as the boundary, was the target area selected for this invention. Figure 2The black box shown in Figure A represents the target area in this embodiment of the invention. In programming software, the soft tissue in the image is first segmented to reduce interference with bone tissue information; then, the morphological data of the trabeculae at the ligament-bone junction are statistically analyzed, including the orientation, number, porosity, and anisotropy of the trabeculae. Finally, the morphological information of the trabeculae at the anterior cruciate ligament attachment is obtained. Figure 2 In Figure B, the morphological information of the trabecular bone at the center point of the anterior cruciate ligament attachment site was extracted using software.
[0072] Step 2: Extract the mechanical properties of the bone tissue at the anterior cruciate ligament attachment site, specifically including:
[0073] like Figure 2 As shown in Figure C, based on the target region from which the morphological information of the trabecular bone at the anterior cruciate ligament (ACL) bone junction was extracted in step one, a detailed dissection was performed on the formalin-treated human knee joint specimen, removing bone and soft tissue outside the target region. The obtained bone tissue at the ACL attachment site was scanned using a Micro-CT imaging device to analyze the parameters of the bone tissue in this area, including bone volume, bone volume fraction, bone surface area, bone surface area-to-volume ratio, and trabecular bone parameters. Then, as follows... Figure 3 As shown in Figures A and B, mechanical tests were performed on the bone tissue to extract the mechanical properties of the bone tissue at the anterior cruciate ligament attachment site.
[0074] Step 3: Restore the original structural features and mechanical properties of the bone tunnel:
[0075] like Figure 3 As shown in Figure C, based on the trabecular bone structure characteristics obtained in Step 1, a model of the fixation screw used in anterior cruciate ligament (ACL) reconstruction was constructed in nTopology software. This model ensured that the screw perfectly matched the original structural characteristics of the bone tunnel location during ACL reconstruction, including the tunnel's orientation and position. The fabricated fixation screw model was then imported into 3D printing software, and a material with similar or matching mechanical properties was selected for subsequent 3D printing of the screw. Based on the melting temperature and operating temperature of the selected material, the fixation screw used for ACL reconstruction was 3D printed. This step yielded a bone biostructure that conformed to the trabecular bone structure characteristics and mechanical properties at the ACL attachment site.
[0076] Step 4: Establishing a suitable microenvironment for tissue cell growth:
[0077] like Figure 4As shown, to modify the non-repulsive and non-affinity properties of some polymers to tissue cells, the obtained solid fixation screws were immersed in a solution containing a specific dose of dopamine for 12 hours with continuous stirring. A biochemical slow-release agent was added to the solution to ensure a suitable aggregation and adsorption environment, allowing dopamine to polymerize and adsorb onto the surface of the fixation screws. Hydroxyapatite was treated using the same steps to obtain polydopamine-modified hydroxyapatite crystals. The polydopamine-modified hydroxyapatite and the polydopamine-modified fixation screws were then immersed in the biochemical slow-release agent for 12 hours with continuous stirring and in a dark environment. Finally, using polydopamine as a medium, hydroxyapatite was adsorbed onto the surface of the fixation screws. The surface of the fixation screws was then modified to give the polymer surface better tissue compatibility. Finally, as shown... Figure 5 As shown, a bone biomaterial based on both mechanical and biological biomimicry was obtained.
[0078] On the other hand, this invention provides a system for preparing bone biostructures in a bidirectional biomechanical and biological manner for a special trabecular region at the tendon / ligament-bone junction, such as... Figure 6 As shown, it includes:
[0079] The morphological parameter extraction unit is used to extract morphological information of bone trabeculae from human specimen slices based on the target region.
[0080] The mechanical parameter extraction unit is used to obtain mechanical parameters of the tendon / ligament-bone junction through anatomical analysis based on human specimens.
[0081] Bionic structural design unit, used to determine bionic structures based on morphological information of trabeculae;
[0082] A bone biomaterial determination unit is used to determine the biomaterials for preparing biomimetic bone biostructures based on the mechanical parameters of the tendon / ligament bone junction.
[0083] A biomimetic bone biostructure fabrication unit is used to fabricate biomimetic bone biostructures based on biomimetic structures and biomaterials.
[0084] Modification units are used to modify the surface of biomimetic bone structures to obtain the final biomimetic bone structure.
[0085] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.
[0086] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for preparing a biomechanically and biologically biomimetic bone structure in a special trabecular region at the tendon / ligament-bone junction, characterized in that, include: Morphological information of trabeculae was extracted from human specimen sections of the target region. Obtain biomechanical parameters of the tendon / ligament-bone junction through anatomical analysis of human specimens; Biomimetic structures are determined based on the morphological information of trabeculae; Biomaterials with biomimetic structures were prepared based on the mechanical parameters of the tendon / ligament-bone junction. Biomimetic bone biostructures are prepared based on biomimetic structures and biomaterials; The surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure. Anatomical analysis of human specimens is used to obtain biomechanical parameters at the tendon / ligament-bone junction, including: Only the bone tissue at the tendon / ligament attachment site and the area containing reactive trabeculae within the bone is preserved; Micro-CT scans were performed on the acquired bone tissue to obtain imaging data of bone emboli; A three-dimensional model is generated based on the imaging data of the bone plug. Mechanical tests are then performed on the three-dimensional model and the solid bone plug to obtain the mechanical parameters at the tendon / ligament-bone junction. The fabrication of biomimetic bone biostructures based on biomimetic structures and biomaterials includes: The biomimetic structure, determined by morphological data of trabeculae, is fabricated using biomaterials based on mechanical parameters at the tendon / ligament-bone junction. The biomimetic structure is then 3D printed at a preset temperature to generate a solid implant, i.e., a biomimetic bone biostructure. The surface of the biomimetic bone structure is modified to obtain the final biomimetic bone structure, including: The solid implant was soaked in a dopamine aqueous solution for 12 hours with continuous stirring, so that the dopamine in the dopamine aqueous solution was converted into polydopamine and adsorbed onto the surface of the solid implant. Hydroxyapatite was soaked in a dopamine aqueous solution for 12 hours with continuous stirring to generate polydopamine-modified hydroxyapatite. The modified hydroxyapatite and the modified solid implant were soaked in a dopamine aqueous solution for 12 hours with continuous stirring and in a dark environment. This process allowed the hydroxyapatite to be adsorbed onto the surface of the solid implant, resulting in a biomimetic bone structure that is both mechanical and biologically similar at the tendon / ligament-bone junction.
2. The method for preparing a biomechanically and biologically biomimetic bone structure in a special trabecular region at the tendon / ligament-bone junction according to claim 1, characterized in that, Morphological information of bone trabeculae was extracted from human specimen slices of the target region, including: Multiple tomographic sections of the human specimen in the target area were prepared using polymer materials and photographed. Using programming software, slice images with the largest attachment area between soft tissue and bone were selected from the tomographic sections according to the course of the soft tissue at the tendon / ligament-bone junction and the way it is attached to the bone, and then digitally processed. Quantitative analysis of trabecular bone in the digitized slice images was performed to obtain morphological information of trabecular bone in the target region.
3. The method for preparing a biomechanically and biologically biomimetic bone structure in a special trabecular region at the tendon / ligament-bone junction according to claim 2, characterized in that, The morphological information includes the orientation, number, porosity, and anisotropy of the trabeculae.
4. A system for fabricating a bone biostructure based on a two-way biomechanical and biological approach in a special trabecular region at the tendon / ligament-bone junction, characterized in that... The method for preparing a bone biostructure with a two-way biomechanical and biological mimicry of a special trabecular bone region at the tendon / ligament-bone junction, as described in any one of claims 1-3, includes: The morphological parameter extraction unit is used to extract morphological information of bone trabeculae from human specimen slices based on the target region. The mechanical parameter extraction unit is used to obtain mechanical parameters of the tendon / ligament-bone junction through anatomical analysis based on human specimens. A biomimetic structural design unit is used to determine the biomimetic structure of the implant based on the morphological information of the trabeculae; The bone biomaterial determination unit is used to determine the biomaterials for preparing biomimetic structures based on the mechanical parameters of the tendon / ligament bone junction. A biomimetic bone biostructure fabrication unit is used to fabricate biomimetic bone biostructures based on biomimetic structures and biomaterials. Modification units are used to modify the surface of biomimetic bone structures to obtain the final biomimetic bone structure.