Hair growth composition containing natural ingredients and preparation method thereof

Abstract

The present invention provides a hair growth composition containing natural components, which does not have cytotoxicity, has excellent alkaline phosphatase activity, and has an excellent hair growth effect when applied to actual scalp, and a method for preparing the same. Therefore, hair loss is prevented, and the composition can be used as hair-growing products.

Description

Technical Field

[0001] This invention relates to a hair growth composition containing natural ingredients and a method for manufacturing the same. Background Technology

[0002] Hair loss refers to a condition in which the proportion of hair growing in telogen (resting) or catagen (transitional) phases of the hair follicles increases compared to the proportion of hair growing in anagen (growth) phases, leading to an abnormal increase in hair loss. In recent years, due to increased emphasis on appearance, people have shown growing interest in hair loss. Causes of hair loss include genetic factors, aging, and stress, but the exact cause remains unclear. Generally, the most typical type of hair loss is male pattern baldness, which occurs due to excessive action of androgens, with representative androgens including testosterone and dihydrotestosterone (DHT). DHT is also known. Testosterone is converted to DHT by 5-α-reductase, a conversion that results in type 1 and type 2. Of the two types of 5-α-reductase, type 2 has been reported to be caused by 5-α-reductase. The converted DHT leads to gradual atrophy of the dermal papilla and hair follicles.

[0003] In the treatment and prevention of [the disease], the most widely used drugs are those containing minoxidil and finasteride, which has type 2 5-α-reductase inhibitory activity.

[0004] However, these medications all have side effects such as decreased sexual function, and the severity of these side effects varies from person to person. Therefore, there is a need for hair regrowth agents based on natural products with fewer side effects. However, no hair regrowth agent has yet been developed that is as effective as existing medications, thus requiring further research.

[0005] Existing technical documents

[0006] Patent documents

[0007] (Patent Document 1) Korean Patent Publication No. 10-1998103 Summary of the Invention

[0008] The problem that the invention aims to solve

[0009] In researching hair growth agents based on natural products, the inventors of this invention used malt, pine needles, and the invention was achieved by confirming that a composition of a combination of concentrate, ginseng, kelp, mulberry bark concentrate, angelica root, nasturtium, perilla leaf and ethanol, prepared under specific conditions, has a significant hair growth effect.

[0010] Therefore, the object of the present invention is to provide a method for manufacturing a topical skin composition for hair growth.

[0011] Another object of the present invention is to provide a topical skin composition for hair growth prepared by the above method.

[0012] means for solving problems

[0013] A method is provided for producing a topical skin-applied composition for hair growth, comprising the following steps:

[0014] a) Prepare a composition by mixing 12% by weight of malt, 20% by weight of pine needle concentrate, 12.2% by weight of ginseng, 13% by weight of kelp, 0.6% by weight of mulberry bark concentrate, 12.2% by weight of angelica root, 10% by weight of nasturtium, 12% by weight of perilla leaf and 8% by weight of ethanol.

[0015] b) Place the composition from step a) into a container and seal it to prevent light from passing through;

[0016] c) The composition from step b) is aged in a sealed state at a temperature of 5–15°C for 9–12 months;

[0017] d) Filter aging composition; and

[0018] e) The composition after distillation and filtration.

[0019] In one embodiment of the present invention, in step c), the temperature is 10°C and the maturation period is 12 months.

[0020] Furthermore, the present invention is manufactured using the above-described manufacturing method and provides a composition for external use on the skin to promote hair growth.

[0021] Invention Effects

[0022] The hair growth composition of the present invention is non-cytotoxic, has excellent alkaline phosphatase activity, and exhibits excellent hair growth effect when applied to the actual scalp, thus it can be effectively used as a hair loss prevention and hair growth product. Attached Figure Description

[0023] Figure 1a , Figure 1b A photograph of the hair of the test subject in Test Example 3 is shown, depicting the hair before application of the hair growth composition of the present invention. Figure 1a ) and 9 months after application ( Figure 1b (The condition of the hair.)

[0024] Figure 2a , Figure 2b Another test example, photograph 3, shows the hair of the test subject before application of the hair growth composition of the present invention. Figure 2a ) and 9 months after application ( Figure 2b (The condition of the hair.) Detailed Implementation

[0025] The present invention will now be described in detail.

[0026] This invention provides a method for producing a topical skin composition for hair growth, comprising the following steps:

[0027] a) Prepare a composition by mixing 12% by weight of malt, 20% by weight of pine needle concentrate, 12.2% by weight of ginseng, 13% by weight of kelp, 0.6% by weight of mulberry bark concentrate, 12.2% by weight of angelica root, 10% by weight of nasturtium, 12% by weight of perilla leaf and 8% by weight of ethanol.

[0028] b) Place the composition from step a) into a container and seal it to prevent light from passing through;

[0029] c) The composition from step b) is aged in a sealed state at a temperature of 5–15°C for 9–12 months;

[0030] d) Filter aging composition; and

[0031] e) The composition after distillation and filtration.

[0032] In this invention, a topical skin agent refers to a pharmacological or cosmetic composition that is applied directly to the skin to exert its effects. These topical agents can be formulated into various dosage forms, such as creams, lotions, gels, sprays, powders, and oils, with each dosage form selected based on the specific skin condition or intended use. Topical skin preparations are typically developed for a variety of skin-related effects, such as moisturizing, anti-inflammatory, soothing, antibacterial, anti-aging, and protective effects, and may contain active ingredients for this purpose.

[0033] These active ingredients may include vitamins, minerals, plant extracts, peptides, and antioxidants, which can help the skin retain moisture, reduce inflammation, promote skin regeneration, and protect against UV rays. In addition, topical skin agents have been developed to address specific skin problems, with added special ingredients to alleviate skin conditions such as acne, psoriasis, and eczema.

[0034] In this invention, hair growth refers to the physiological process of new hair growing from hair follicles present on the scalp, and includes concepts such as increasing cell proliferation in the hair follicle, causing the hair follicle to enter the anagen phase, increasing hair density, and improving hair thickness.

[0035] In this invention, malt is the raw material made from the outer barley through germination and drying, and is referred to as barley. During the germination process, the starch in the grain is broken down by enzymes (especially α-amylase) and converted into monosaccharides. Through this process, malt provides soluble sugars that yeast can ferment.

[0036] In this invention, a concentrate refers to a substance produced by extracting raw materials such as pine needles or Sichuan pepper bark using various solvents, and then removing water from the extract to increase its concentration. This may mean reducing the moisture content to 20% or lower. Preferably, this may mean reducing the moisture content to 10%.

[0037] In this invention, the pepper bark is a material extracted from the outer bark of the pepper tree. The mulberry tree is a tree that grows particularly in East Asia, and its bark has traditionally been used as a spice and medicine. Mulberry bark has a rich aroma and spicy flavor, primarily due to terpenoid compounds such as limonene and pinene.

[0038] In this invention, the golden lotus is an aquatic plant belonging to the Nelumbo nucifera family. It is a perennial plant that mainly grows in ponds and lakes in Asia and the Middle East. All parts of the plant, including the flowers, leaves, seeds, and roots (lotus root), can be used for edible, medicinal, and ornamental purposes. In particular, its seeds and roots are widely used in traditional Asian cuisine and medicine.

[0039] In this invention, perilla leaves refer to the leaves of the perilla plant, which belongs to the Lamiaceae family and is widely used for edible and medicinal purposes, primarily in East Asia. Perilla leaves have a unique aroma and flavor and possess anti-inflammatory, antioxidant, and anti-allergic properties. The leaves of this plant can be used in various dishes and are consumed as a dietary supplement and traditional medicine, offering benefits such as cough relief, immune enhancement, digestive aid, and mood stabilization.

[0040] Step a) is the step of preparing the natural raw materials. These raw materials can be commercially available. Preparatory work is required before step a). The raw materials can be concentrated by thermal extraction from distilled water followed by reducing the moisture content.

[0041] The root of ginseng and dried kelp are preferred. The entire leaves of nasturtium and perilla can be used, with the leaves being the best choice.

[0042] Step b) is the step of placing the composition into a container and sealing it. A tight seal is important because the components contained in the composition may deteriorate when exposed to light. The material used for sealing can be applied without restriction, as long as it is opaque.

[0043] Step c) is the aging step, which can be carried out in a sealed state at a temperature of 5°C to 15°C for 9 to 12 months. Preferably, it can be aged at 10°C for 12 months. As can be seen from Test Example 2, the alkaline phosphatase activity is most significant under the above conditions.

[0044] Step d) is the step of removing foreign matter from the composition, and conventional methods, such as filtration through filter paper, can be applied.

[0045] Step e) is a distillation step, in which the composition from which foreign matter has been removed in step d) can be distilled using a distiller and a cooling tower to remove impurities.

[0046] Furthermore, this invention provides a composition for topical application to the skin to promote hair growth, manufactured by a manufacturing method. As described in the test examples below, this composition exhibits excellent alkaline phosphatase activity, is non-cytotoxic, and demonstrates excellent hair growth effects when applied to the actual scalp.

[0047] The following embodiments explain the invention in more detail. These embodiments are intended to illustrate the invention, and the scope of the invention should not be limited thereto.

[0048] Manufacturing Example 1: Material Preparation

[0049] Prepare 70% ethanol from 120g malt, 200g pine needle concentrate, 122g ginseng, 130g kelp, 6g mulberry bark concentrate, 122g angelica, 100g golden lotus, 120g perilla leaves, and 80g of other ingredients.

[0050] The raw materials are placed in distilled water at 10 times their weight and heated to 100°C to reduce the moisture content to 10%, thus preparing pine needle concentrate and mulberry bark concentrate. For ginseng, the root portion after removing impurities is used. Fully dried kelp is used. The leaf portions of nasturtium and perilla are used.

[0051] Preparation Example 2: Preparation of Hair Growth Composition

[0052] In Preparation Example 1, 120g of malt, 200g of pine needle concentrate, 122g of ginseng, 130g of kelp, 6g of mulberry bark concentrate, 122g of angelica, 100g of golden lotus, 120g of perilla leaves, and 80g of 70% ethanol were thoroughly mixed and then added to an opaque solution. The mixture was placed in a jar, sealed with black plastic to prevent light transmission, and then aged. Samples for different aging periods of 6 months, 9 months, 12 months, and 15 months were prepared at 20°C and 15°C, respectively, with aging temperatures of 1°C, 5°C, 10°C, and 15°C. After aging, foreign matter was removed by filtration through 20µm filter paper, and the composition was obtained by cooling in a tower while simultaneously heating to 100°C in a distiller to prepare a composition for hair growth.

[0053] Test Example 1: Viability Experiment of Full Flow Cell

[0054] The CCK-8 assay, reflecting cell number, is used to measure cell growth and proliferation. The CCK-8 assay was used to test the cytotoxicity of each sample from Preparation Example 2 against human dermal papillary cells. Specifically, 3 × 10⁻⁶ samples were... 3Dermal papillary cells were cultured in 96-well plates for 24 hours, then replaced with serum-free medium to remove growth factors. 1 ml of the sample prepared for Example 2 was processed. An equal volume of DMSO was used as the negative control. After 72 hours of culture, CCK8 cells were treated, and cell growth was analyzed by measuring absorbance at 450 nm. The absorbance value of the control group was calculated. The relative absorbance of the samples was converted to 100%, as shown in the table below.

[0055] Table 1

[0056]

[0057] As shown in Table 1, the hair growth composition of the present invention does not inhibit the growth of hair papilla cells and therefore is not cytotoxic.

[0058] Test Example 2: Alkaline Phosphatase (ALP) Activity Assay

[0059] ALP (alkaline phosphatase) is known as an indicator of angiogenesis in the hair growth cycle and is used as an indicator of hair growth because the enzyme activity increases when hair is induced to enter the growth phase.

[0060] The alkaline phosphatase (ALP) activity of the compositions of the present invention was measured using an alkaline phosphatase assay kit (SensoLyte, Anaspec). Dermal papillary cells were dispensed into 6-well plates (1 x 10⁻⁶). 5 The samples were then incubated at 37°C for 24 hours in a CO2 incubator, and then incubated for 48 hours and 120 hours respectively in wells. Next, using cell lysis buffer, the samples were evaluated using an ELISA reader (SoftMax Pro5, Molecular) according to the instructions of the alkaline phosphatase assay kit. The absorbance at 405 nm was measured using Devices (USA). Additionally, the same breast milk cells were treated only with DMSO (dimethyl sulfoxide) as a control group, and the absorbance value of the control group was set to 100%. The relative absorbance of the samples was then converted.

[0061] Table 2

[0062]

[0063] As shown in Table 2 above, excellent ALP activity was observed at temperatures between 5 and 15°C when the child was 9 to 12 months old. In particular, the ALP activity was significantly superior at 10°C for 12 months of aging, resulting in a significant hair regrowth effect.

[0064] Test Example 3: Confirming the hair regrowth effect of the composition of the present invention on hair loss symptoms.

[0065] The hair growth effect of the composition of the present invention was confirmed by a study on people in their 50s and 60s with hair loss symptoms. In test example 2, the sample aged at 10°C for 12 months was the most effective, using 15 men as subjects.

[0066] Specifically, test subjects were instructed to apply 10 ml of the sample to their scalp once daily. Nine months after the first use, hair density in the balding areas was measured and averaged. Effective hair thickness was set to be at least 20 μm during measurement.

[0067] Table 3

[0068] Initial application date Nine months later <![CDATA[Hair loss area: 1 cm 2 Hair density]]> 33.1 pieces 67.5 pieces

[0069] As shown in Table 3 above, when the composition of the present invention is applied to the scalp for a long period of time, the number of hair strands per unit area increases significantly, confirming that the composition of the present invention is excellent at improving hair loss and promoting hair growth.

Claims

1. A method for preparing a hair growth composition, wherein, The method for preparing the hair growth composition includes the following steps: a) Prepare a composition by mixing 12% by weight of malt, 20% by weight of pine needle concentrate, 12.2% by weight of ginseng, 13% by weight of kelp, 0.6% by weight of mulberry bark concentrate, 12.2% by weight of angelica root, 10% by weight of nasturtium, 12% by weight of perilla leaf and 8% by weight of ethanol. b) Place the composition from step a) into a container and seal it to prevent light from passing through; c) The composition from step b) is aged in a sealed state at a temperature of 5–15°C for 9–12 months; d) Filter aging composition; and e) The composition after distillation and filtration.

2. The method for preparing the hair growth composition according to claim 1, wherein, In step c), the temperature is 10°C and the maturation period is 12 months.

3. A hair growth composition, wherein, The hair growth composition is prepared by the method for preparing the hair growth composition according to claim 1 or 2.

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