Natural preservative composition containing cinnamon bark extract as well as preparation method and application of natural preservative composition

Abstract

The invention belongs to the technical field of natural anti-corrosion materials, and provides a natural anti-corrosion composition containing cinnamon bark extract and a preparation method and application of the natural anti-corrosion composition. The natural preservative composition provided by the invention comprises a cinnamon bark extract and a synergistic compound, the synergistic compound comprises a mangnolia officinalis bark extract, a perilla leaf extract, a rhizoma atractylodis root extract, a folium artemisiae argyi extract, a wrinkled gianthyssop herb extract, a radix ophiopogonis root extract, a pomegranate seed extract, a pomelo extract, a thyme leaf extract, a calendula extract, a hyperforin perforatum extract, a melaleuca alternifolia leaf extract, a ginger root extract and an eucalyptus globulus leaf extract; the mass content of cinnamyl aldehyde in the cinnamon bark extract is less than or equal to 20wt%, and the antibacterial composition has the advantages of broad-spectrum bacteriostasis, high stability, difficulty in discoloration and precipitation, acid and alkali resistance, high temperature resistance, safety and small irritation. The antibacterial composition is prepared from at least one of a ginseng root extract, a rosemary leaf extract, a papaya fruit extract, a chamomile flower extract, a soybean seed extract and a wheat bran extract, and the mass content of cinnamyl aldehyde in the cinnamon bark extract is less than or equal to 20wt%.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of natural preservative materials, and more particularly to a natural preservative composition containing cinnamon bark extract and a preparation method and application thereof. BACKGROUND

[0002] There are a large number of nutrients in cosmetics, which are prone to be contaminated by microorganisms during production and use, resulting in product deterioration. The existing technology usually avoids contamination by adding chemical preservatives. However, the conventional chemical preservatives commonly used in cosmetics have certain safety hazards (such as formaldehyde release, strong irritation, carcinogenicity, etc.), leading consumers to prefer products using natural preservatives instead of chemical preservatives.

[0003] At present, a single plant extract is commonly used as a preservative, but its preservative effect is not good (it cannot achieve broad-spectrum antibacterial effect, and the dosage is large), and it has poor stability (it is prone to discoloration and precipitation), and it is difficult to apply to products (it has a strong odor and a deep color). For example, known plant extracts with good antibacterial effect, such as cinnamon bark extract, usually contain a high concentration of cinnamaldehyde (the mass content of cinnamaldehyde in cinnamon bark extract is usually ≥80 wt%), which has strong skin irritation and a strong odor, and direct addition to cosmetics as an antibacterial agent can easily cause sensory defects.

[0004] Therefore, there is an urgent need to develop a natural preservative with strong antibacterial property, high efficiency, excellent stability, safety, and without affecting the sensory experience of cosmetics. SUMMARY

[0005] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a natural preservative composition containing cinnamon bark extract and a preparation method and application thereof. The natural preservative composition provided by the present application has high-efficiency antibacterial performance and excellent preservative effect, and has high stability under different conditions, such as acid (pH=3, pH=6), alkaline (pH=8, pH=10), high temperature (60℃), low temperature (4℃, -4℃), heating (90℃, 150℃), and has excellent antibacterial ability and maintains normal appearance. In addition, the raw material components are all extracts of natural plants, which are safe and can be further used in cosmetics and care products without affecting the sensory experience of the cosmetics themselves.

[0006] The first aspect of the present application provides a natural preservative composition.

[0007] Specifically, a natural preservative composition comprises the following components: cinnamon bark extract and synergistic compound. The synergistic compound comprises at least one of the following: Magnolia officinalis bark extract, Perilla frutescens leaf extract, Atractylodes lancea root extract, Artemisia argyi leaf extract, Pogostemon cablin extract, Ophiopogon japonicus root extract, Punica granatum seed extract, Pomegranate extract, Thyme leaf extract, Calendula officinalis extract, St. John's wort extract, Melaleuca alternifolia leaf extract, Zingiber officinale root extract, Eucalyptus globulus leaf extract, Panax ginseng root extract, Rosemary leaf extract, Papaya fruit extract, Matricaria champaca flower extract, Soybean seed extract, and Wheat bran extract; wherein the cinnamaldehyde content in the cinnamon bark extract is ≤20wt%.

[0008] The natural preservative composition provided by this invention uses cinnamon bark extract combined with other specific plant extracts as synergistic compounds. It not only has excellent antibacterial ability, but also has natural ingredients, high safety and high stability. When used in cosmetics, it can play a highly effective preservative role. Moreover, the mass content of cinnamaldehyde is low, so it will not affect the sensory experience of the cosmetic itself.

[0009] Preferably, the natural preservative composition comprises, by weight percentage, the following raw material components: 50-85% cinnamon bark extract and 15-50% synergistic compound.

[0010] More preferably, the natural preservative composition comprises, by weight percentage, the following raw material components: 55-80% cinnamon bark extract and 20-45% synergistic compound.

[0011] Preferably, the synergistic compound includes at least one of the following: Atractylodes lancea root extract, thyme leaf extract, perilla leaf extract, Magnolia officinalis bark extract, Ophiopogon japonicus root extract, Artemisia argyi leaf extract, and pomegranate seed extract.

[0012] More preferably, the synergistic compound includes at least one of Atractylodes lancea root extract, thyme leaf extract, perilla leaf extract, Magnolia officinalis bark extract, and Ophiopogon japonicus root extract.

[0013] More preferably, the components of the natural preservative composition include cinnamon bark extract, Atractylodes lancea root extract, thyme leaf extract, perilla leaf extract, Magnolia officinalis bark extract, and Ophiopogon japonicus root extract; or, The components of the natural preservative composition include cinnamon bark extract, thyme leaf extract, and magnolia bark extract.

[0014] More preferably, the natural preservative composition comprises, by weight, 55-70 parts of cinnamon bark extract, 5-10 parts of Atractylodes lancea root extract, 5-20 parts of thyme leaf extract, 5-10 parts of perilla leaf extract, 1-10 parts of Magnolia officinalis bark extract, and 1-5 parts of Ophiopogon japonicus root extract. or, The natural preservative composition comprises, by weight, 60-85 parts of cinnamon bark extract, 8-20 parts of thyme leaf extract, and 8-20 parts of magnolia bark extract.

[0015] More preferably, the natural preservative composition comprises, by weight, 60-70 parts of cinnamon bark extract, 8-10 parts of Atractylodes lancea root extract, 10-13 parts of thyme leaf extract, 8-10 parts of perilla leaf extract, 5-6 parts of Magnolia officinalis bark extract and 2-5 parts of Ophiopogon japonicus root extract. or, The natural preservative composition comprises, by weight, 70-80 parts of cinnamon bark extract, 10-20 parts of thyme leaf extract, and 10-20 parts of magnolia bark extract.

[0016] Preferably, the cinnamaldehyde content in the cinnamon bark extract is 5-15 wt%.

[0017] More preferably, the cinnamaldehyde content in the cinnamon bark extract is 5-8 wt%.

[0018] A second aspect of the present invention provides a method for preparing a natural preservative composition.

[0019] A method for preparing a natural preservative composition includes the following steps: The components are mixed to obtain the natural preservative composition.

[0020] Preferably, the method for preparing the cinnamon bark extract includes the following steps: Cinnamon bark was crushed and then steam distilled to obtain a primary extract. The extract was then eluted and the fraction was collected to obtain the cinnamon bark extract.

[0021] Preferably, silica gel is used as the adsorbent for the elution.

[0022] Preferably, the eluent is a mixed solvent of petroleum ether and ethyl acetate.

[0023] Preferably, the elution is gradient elution.

[0024] Preferably, the preparation method of the Atractylodes lancea root extract includes the following steps: The Atractylodes lancea root was pulverized and then steam distilled to obtain the Atractylodes lancea root extract.

[0025] Preferably, the method for preparing the thyme leaf extract includes the following steps: Thyme leaves are pulverized and then steam distilled to obtain the thyme leaf extract.

[0026] Preferably, the preparation method of the perilla leaf extract includes the following steps: The perilla leaves were pulverized and then steam distilled to obtain the perilla leaf extract.

[0027] Preferably, the preparation method of the Magnolia officinalis bark extract includes the following steps: Magnolia officinalis bark was pulverized and then steam distilled to obtain the Magnolia officinalis bark extract.

[0028] Preferably, the preparation method of the Ophiopogon japonicus root extract includes the following steps: The root of Ophiopogon japonicus was pulverized and then steam distilled to obtain the Ophiopogon japonicus root extract.

[0029] A third aspect of the present invention provides an application of a natural preservative composition.

[0030] The use of a natural preservative composition in the preparation of cosmetic or care products.

[0031] Preferably, the cosmetic is a toner, serum, mask, lotion, or cleanser.

[0032] Preferably, the care product is a shower gel, shampoo, toothpaste, or mouthwash.

[0033] Preferably, in the cosmetic, the added natural preservative composition accounts for 0.08-0.5 wt% of the total mass of the cosmetic.

[0034] More preferably, in the cosmetic, the added natural preservative composition accounts for 0.1-0.3 wt% of the total mass of the cosmetic.

[0035] Preferably, in the care product, the added natural preservative composition accounts for 0.08-0.5 wt% of the total mass of the care product.

[0036] More preferably, in the care product, the added natural preservative composition accounts for 0.1-0.3 wt% of the total mass of the care product.

[0037] Compared with the prior art, the beneficial effects of the present invention are as follows: The natural preservative composition provided by this invention uses cinnamon bark extract and synergistic compound as main components, wherein the synergistic compound includes at least one of the following: Magnolia officinalis bark extract, Perilla frutescens leaf extract, Atractylodes lancea root extract, Artemisia argyi leaf extract, Pogostemon cablin extract, Ophiopogon japonicus root extract, pomegranate seed extract, Pomelo truncata extract, Thyme leaf extract, Calendula officinalis extract, St. John's wort extract, Melaleuca alternifolia leaf extract, Zingiber officinale root extract, Eucalyptus globulus leaf extract, Ginseng root extract, Rosemary leaf extract, Papaya fruit extract, Matricaria champaca extract, Soybean seed extract, and wheat bran extract; the cinnamaldehyde content in the cinnamon bark extract is ≤20wt%. The natural preservative composition of this invention achieves broad-spectrum antibacterial effects, for example, inhibiting Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, Propionibacterium acnes, and Malassezia furfur. It also exhibits good stability, maintaining its antibacterial effect under acidic, alkaline, and high / low temperature conditions (e.g., acidic conditions at pH 3 and 6, alkaline conditions at pH 8 and 10, high temperatures at 60°C, low temperatures at 4°C and -4°C, and heating conditions at 90°C and 150°C). It is not prone to discoloration or precipitation, and is resistant to acids, alkalis, and high temperatures. All raw materials used in this invention are of natural origin, ensuring safety and minimizing irritation. The resulting cosmetics and other products possess excellent sensory properties and will not adversely affect the sensory performance of the products themselves. Therefore, the natural preservative composition of this invention has a wide range of applications (e.g., in pure water systems, emulsion systems, and surfactant systems). Attached Figure Description

[0038] Figure 1 The image shows the sensory evaluation results of the essence made from the self-made cinnamon bark extract of this invention and the essence made from commercially available cinnamon bark extract. Detailed Implementation

[0039] To enable those skilled in the art to more clearly understand the technical solutions described in this invention, the following embodiments are provided for illustration. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by this invention.

[0040] Unless otherwise specified, the raw materials, reagents or devices used in the following examples are available from conventional commercial sources or can be obtained by existing known methods.

[0041] The main raw materials used in this invention are sourced from the following sources: The preparation method for homemade cinnamon bark extract is as follows: (1) Crush the dried cinnamon bark, put it into a still, and distill it by steam to obtain cinnamon bark essential oil extract; (2) Taking advantage of the difference in adsorption capacity of cinnamaldehyde and other components on the stationary phase, most of the cinnamaldehyde is removed. Specifically, the cinnamon bark essential oil extract is dried with anhydrous sodium sulfate (to remove moisture and avoid cracking of the silica gel column), and then diluted with petroleum ether to a suitable concentration. (3) Use silica gel as an adsorbent. Select 200-300 mesh silica gel, mix it with petroleum ether, silica gel: petroleum ether = 1:1 (volume ratio), pack it into the chromatography column, and press it with double balls. The volume of the column bed is about nine-tenths of the volume of silica gel to avoid cracking of the column bed. (4) Slowly inject the pretreated sample into the top of the column to avoid dispersing the silica gel bed, and then rinse the top of the column with 50 mL of petroleum ether to remove unadsorbed impurities. (5) Using a gradient elution strategy, elute sequentially with the following eluents from weakly polar to strongly polar, collecting one fraction per 100 mL of eluent: ① Petroleum ether-ethyl acetate (95:5, volume ratio), weakly polar, used to elute cinnamaldehyde; ② Petroleum ether-ethyl acetate (85:15, volume ratio), medium to weak polarity, eluting a mixture of cinnamaldehyde and cinnamyl alcohol; ③ Petroleum ether-ethyl acetate (75:25, volume ratio), neutral polarity, eluting cinnamyl alcohol; ④ Methanol-chloroform (80:20, volume ratio), highly polar, washes away residual polar impurities; (6) Each fraction was analyzed by thin-layer chromatography (TLC); (7) Combine fractions ② and ③, and concentrate under reduced pressure using a rotary evaporator to remove the solvent, yielding a cinnamon bark extract (cinnamon bark essential oil extract) with low cinnamaldehyde content and high cinnamyl alcohol content. The cinnamaldehyde content is 5-8 wt%. The main components of the self-made cinnamon bark extract include: cinnamyl alcohol, cinnamaldehyde, eugenol, benzaldehyde, and p-isopropyltoluene.

[0042] Commercially available cinnamon bark extract: cinnamaldehyde content ≥80wt%, manufactured by Maclean, product number C689971.

[0043] Preparation method of Atractylodes lancea root extract: The dried Atractylodes lancea root is pulverized and placed in a distiller for steam distillation to obtain the Atractylodes lancea root essential oil extract. The main components of the product include: β-cineole, atractylol, and atractylone.

[0044] Preparation method of thyme leaf extract: Dried thyme leaves are pulverized and placed in a still, then distilled by steam to obtain thyme leaf essential oil extract. The main components of the product include: thymol, p-cymene, γ-terpinene, caryophyllene, and linalool.

[0045] Preparation method of perilla leaf extract: Dried perilla leaves are pulverized and placed in a distiller for steam distillation to obtain perilla leaf essential oil extract. The main components of the product include: perillaldehyde, perillyl alcohol, caryophyllene, and citral.

[0046] Preparation method of Magnolia officinalis bark extract: The dried Magnolia officinalis bark is pulverized and placed in a still, then distilled by steam to obtain Magnolia officinalis bark essential oil extract. The main components of the product include: magnolol and honokiol.

[0047] Preparation method of Ophiopogon japonicus root extract: The dried Ophiopogon japonicus root is pulverized and placed in a distiller for steam distillation to obtain Ophiopogon japonicus root essential oil extract. The main components of the product include: tarragon, linalool, pinene, caryophyllene, citronellol, and palmitic acid.

[0048] Commercially available preservative composition: The main ingredient is cinnamon bark extract, specifically composed of the following components by weight percentage: 80% cinnamon bark extract, 5% rosemary leaf extract, 5% citrus peel extract, 5% soybean seed extract, 3% cassia seed extract, and 2% peppermint leaf extract. The commercially available preservative composition is prepared by first extracting each plant essential oil separately using steam distillation, and then mixing the plant essential oils at room temperature.

[0049] Base oil: The specific type is sweet almond oil, manufactured by Maclean's, and model number S885962.

[0050] Example 1 A natural preservative composition, the components of which are shown in Table 1 below.

[0051] The preparation method of the above-mentioned natural preservative composition includes the following steps: The components are mixed evenly at room temperature to obtain a natural preservative composition.

[0052] Example 2 A natural preservative composition, the components of which are shown in Table 1 below. The preparation method is the same as in Example 1.

[0053] Example 3 A natural preservative composition, the components of which are shown in Table 1 below. The preparation method is the same as in Example 1.

[0054] Example 4 A natural preservative composition, the components of which are shown in Table 1 below. The preparation method is the same as in Example 1.

[0055] Comparative Examples 1-7 (single components) Comparative Examples 1-7 provide single-component natural preservatives, the specific components and dosages of which are shown in Table 1.

[0056] Comparative Example 8 Comparative Example 8 provides a natural preservative composition, the specific components and amounts of which are shown in Table 1. The preparation method is the same as in Example 1.

[0057] Comparative Example 9 (Commercially Available) Comparative Example 9 provides a commercially available anti-corrosion composition.

[0058] The components and their amounts in the above embodiments and comparative examples are shown in the table below.

[0059] Table 1. Components and their amounts (parts by weight) of each embodiment and comparative example.

[0060] In Table 1, the total weight of the components in each embodiment and comparative example is 100 parts.

[0061] Product effectiveness test 1. Antibacterial ability test Test method: "Disinfection Technical Specifications-2002 2.1.8.4 Minimum Inhibitory Concentration Determination Test - Nutrient Broth Dilution Method".

[0062] Test results: Table 2. Antibacterial activity test results of each example and comparative example (minimum inhibitory concentration MIC, unit mg / L)

[0063] The results in the table above show that: (1) The natural preservative compositions prepared in Examples 1 and 2 of the present invention have excellent antibacterial ability.

[0064] Examples 3 and 4 were slightly less effective than Examples 1 and 2, but significantly more effective than Comparative Examples 1-9.

[0065] (2) Compared with Example 1, the antibacterial ability of Comparative Examples 1 and 2, which contained only a single cinnamon bark extract (whether homemade or commercially available), was significantly reduced. Furthermore, comparing Comparative Examples 1 and 2, it can be seen that the antibacterial ability of the homemade cinnamon bark extract was lower than that of the commercially available cinnamon bark extract, because the homemade cinnamon bark extract contained a lower content of cinnamaldehyde.

[0066] (3) As can be seen from Example 1 and Comparative Examples 1-7, the antibacterial effect of using a single plant extract is weaker than the antibacterial effect of a combination of specific types of extracts in Example 1.

[0067] (4) As can be seen from the comparison between Example 1 and Comparative Example 8, the antibacterial effect will be greatly reduced without the addition of homemade cinnamon bark extract.

[0068] (5) As can be seen from the comparison of Example 1, Example 2 and Comparative Example 9, the antibacterial effect of the natural preservative composition obtained by the present invention using specific types of extracts is better than that of commercially available preservative compositions.

[0069] 2. Stability Test Test method: The natural preservative compositions of Examples 1 and 2 were placed under different conditions to test their preservative ability in order to evaluate their stability in different environments. The specific test conditions are shown in the table below, and the specific test methods refer to "Disinfection Technical Specifications-2002 2.1.8.4 Minimum Inhibitory Concentration Determination Test Nutrient Broth Dilution Method".

[0070] Test results: Table 3. Antibacterial activity test results of the natural preservative composition of Example 1 in different environments.

[0071] Table 4. Antibacterial activity test results of the natural preservative composition of Example 2 in different environments.

[0072] In this context, "normal appearance" indicates that there were no adverse phenomena such as precipitation, sedimentation, or stratification during the test. Otherwise, it is considered abnormal.

[0073] As shown in the table above, the natural preservative compositions of Examples 1 and 2 of the present invention exhibit excellent antibacterial ability under acidic (pH=3, pH=6), alkaline (pH=8, pH=10), high temperature (60℃), low temperature (4℃, -4℃), and heating (90℃, 150℃) conditions. They also maintain a normal appearance and are not prone to discoloration or precipitation. The natural preservative compositions are highly stable, resistant to acids and alkalis, and resistant to high temperatures, and can exert excellent antibacterial performance under different environmental conditions.

[0074] 3. Practical application performance: Cosmetics Test method: The natural preservative composition of Example 1 was used to prepare serum, lotion and cleanser respectively. Then the microbial growth of serum, lotion and cleanser was tested respectively. The antibacterial ability of natural preservative composition in practical application was evaluated with reference to "T / GDCDC 010-2019 Cosmetic Preservative Challenge Test Method".

[0075] Test Principle and Application: By artificially inoculating representative microorganisms (bacteria and fungi) into cosmetics, the number of surviving bacteria is continuously counted at specified time points, and the inhibition / killing dynamics of these microorganisms by the preservative system in the formulation are observed. This allows for the determination of the microbial safety and stability of the formulation throughout its entire lifecycle. This method can simulate primary / secondary contamination scenarios during production and use, and is one of the core pieces of evidence demonstrating the effectiveness of the preservative system in safety assessments.

[0076] Test results: (1) Essence The components and dosages of the essence are shown in the table below.

[0077] Table 5. Components and their content (wt%) in the essence

[0078] The results of the preservative challenge test for the essence are shown in the table below.

[0079] Table 6 Results of the 28-day Preservative Challenge Test of the Essence

[0080] In the table, "ND" indicates not detected.

[0081] As shown in the table above, the experimental group (containing the essence of the natural preservative composition of Example 1 of this invention) passed the preservation challenge test because the colony count at each testing time met the standard requirements (the colony count decreased to the standard requirement value), while the control group failed the preservation challenge test. This indicates that the natural preservative composition of Example 1 can effectively exert its preservative and antibacterial effects and has the ability to resist certain microbial contamination within its shelf life.

[0082] (2) Emulsion The components and amounts in the emulsion are shown in the table below.

[0083] Table 7. Components and dosage (wt%) in the emulsion

[0084] The results of the preservative challenge test for the emulsion are shown in the table below.

[0085] Table 8 Results of the 28-day preservative challenge test of the emulsion

[0086] In the table, "ND" indicates not detected.

[0087] As shown in the table above, the emulsion containing the natural preservative composition of Example 1 of this invention (experimental group) met the standard requirements for colony count at each testing time (colony count decreased to the standard requirement value) and passed the preservation challenge test, while the control group failed the preservation challenge test. This indicates that the natural preservative composition of Example 1 can effectively exert its preservative and antibacterial effects and has the ability to resist certain microbial contamination during the shelf life.

[0088] (3) Facial cleanser The components and dosages of the facial cleanser are shown in the table below.

[0089] Table 9. Components and dosage (wt%) in facial cleanser

[0090] The results of the preservative challenge test for the facial cleanser are shown in the table below.

[0091] Table 10 Results of the 28-day preservative challenge test for facial cleansers

[0092] In the table, "ND" indicates not detected.

[0093] As shown in the table above, the facial cleanser (experimental group) containing the natural preservative composition of Example 1 of this invention met the standard requirements for colony count at each testing time (colony count decreased to the standard requirement value) and passed the preservation challenge test, while the control group failed the preservation challenge test. This indicates that the natural preservative composition of Example 1 can effectively exert its preservative and antibacterial effects and has the ability to resist certain microbial contamination within its shelf life.

[0094] 4. Practical application performance: nursing products Test Method: Anti-dandruff shampoo and mouthwash were prepared using the natural preservative composition from Example 1. The tests were conducted according to the method outlined in WS / T650-2019, "Evaluation Method for Antibacterial and Bacteriostatic Effects 5.1.1 Quantitative Suspension Antibacterial Test". The antibacterial ability of the natural preservative composition in practical applications was evaluated by mixing the tested antibacterial product with a known concentration of microbial suspension. The antibacterial effect was assessed by culturing and determining the number of surviving bacteria and calculating the inhibition rate. An inhibition rate ≥50%-90% was considered to have an antibacterial effect; an inhibition rate ≥90% was considered to have a strong antibacterial effect.

[0095] Test results: (1) Anti-dandruff shampoo Dandruff production is closely related to the balance of the scalp microbiota, particularly the excessive proliferation of Malassezia furfur. Anti-dandruff shampoos, by adding antibacterial ingredients, effectively reduce the number of excessively proliferating Malassezia furfur on the scalp, thereby interrupting the chain of scalp inflammation and abnormal keratin metabolism caused by the fungus. Therefore, the antibacterial effect against Malassezia furfur is a commonly used method for evaluating the efficacy of anti-dandruff shampoos.

[0096] The components and dosages of anti-dandruff shampoo are shown in the table below.

[0097] Table 11. Components and their content (wt%) in anti-dandruff shampoo

[0098] The antibacterial rate test results of the antidandruff shampoo are shown in the table below.

[0099] Table 12 Results of antibacterial rate test for anti-dandruff shampoos

[0100] As shown in the table above, the anti-dandruff shampoo containing the natural preservative composition of Example 1 of the present invention has a strong antibacterial effect against Malassezia furfur.

[0101] (2) Mouthwash The components and dosage of mouthwash are shown in the table below.

[0102] Table 13. Components and their content (wt%) in mouthwash

[0103] The antibacterial rate test results of the mouthwash are shown in the table below.

[0104] Table 14. Results of antibacterial rate test for mouthwash

[0105] As can be seen from the results in the table above, the mouthwash (experimental group) containing the natural preservative composition of Example 1 of the present invention has a strong antibacterial effect against Escherichia coli, Staphylococcus aureus, and Candida albicans.

[0106] 5. Sensory testing Test Method: Homemade cinnamon bark extract and commercially available cinnamon bark extract were each formulated into an essence with a mass fraction of 0.1 wt% (the components of the essence are shown in Table 5, the difference being that the natural preservative composition of Example 1 was replaced with either the homemade cinnamon bark extract or the commercially available cinnamon bark extract, with the homemade cinnamon bark extract accounting for 0.1 wt% of the total mass of the essence, and the commercially available cinnamon bark extract accounting for 0.1 wt% of the total mass of the essence). Fifteen participants were selected for a sensory test using a questionnaire. The questionnaire included ratings on mildness and non-irritation, color, odor, skin feel, and liking, with scores ranging from 0 to 5. Higher scores indicated greater satisfaction, and 0 indicated the least satisfaction.

[0107] Test results: such as Figure 1 As shown, serums containing homemade cinnamon bark extract scored higher than those containing commercially available cinnamon bark extract in terms of gentleness, non-irritation, odor, skin feel, and overall user preference, indicating that homemade cinnamon bark extract is less irritating. Commercially available cinnamon bark extract typically contains high concentrations of cinnamaldehyde, which is highly irritating to the skin and has a strong odor; adding it directly to cosmetics as an antibacterial agent would result in sensory defects.

Claims

1. A natural preservative composition characterized in that, The natural preservative composition comprises the following components: Cinnamomum cassia bark extract and synergistic compound. The synergistic compound comprises at least one of Magnolia officinalis bark extract, Perilla frutescens leaf extract, Atractylodes lancea root extract, Artemisia argyi leaf extract, Agastache rugosa extract, Ophiopogon japonicus root extract, Punica granatum seed extract, Citrus grandis extract, Satureja montana leaf extract, Calendula officinalis extract, Hypericum perforatum extract, Melaleuca alternifolia leaf extract, Zingiber officinale root extract, Eucalyptus globulus leaf extract, Panax ginseng root extract, Rosmarinus officinalis leaf extract, Carica papaya fruit extract, Matricaria chamomilla flower extract, Glycine soja seed extract, and Triticum vulgare wheat bran extract; and the mass content of cinnamaldehyde in the Cinnamomum cassia bark extract is less than or equal to 20 wt%.

2. The natural preservative composition according to claim 1, characterized in that, The natural preservative composition comprises the following components: Cinnamomum cassia bark extract and synergistic compound.

3. The natural preservative composition according to claim 1, wherein The synergistic compound comprises at least one of Magnolia officinalis bark extract, Perilla frutescens leaf extract, Atractylodes lancea root extract, Artemisia argyi leaf extract, Agastache rugosa extract, Ophiopogon japonicus root extract, and Punica granatum seed extract.

4. The natural preservative composition according to claim 3, wherein The synergistic compound comprises at least one of Magnolia officinalis bark extract, Perilla frutescens leaf extract, Atractylodes lancea root extract, Artemisia argyi leaf extract, Agastache rugosa extract, Ophiopogon japonicus root extract, and Punica granatum seed extract.

5. The natural preservative composition according to claim 3, wherein The components of the natural preservative composition comprise Cinnamomum cassia bark extract, Atractylodes lancea root extract, Magnolia officinalis bark extract, Perilla frutescens leaf extract, and Ophiopogon japonicus root extract. Alternatively, The components of the natural preservative composition comprise Cinnamomum cassia bark extract, Magnolia officinalis bark extract, and Perilla frutescens leaf extract.

6. The natural preservative composition according to claim 5, wherein The components of the natural preservative composition comprise Cinnamomum cassia bark extract 55-70 parts, Atractylodes lancea root extract 5-10 parts, Magnolia officinalis bark extract 5-20 parts, Perilla frutescens leaf extract 5-10 parts, Magnolia officinalis bark extract 1-10 parts, and Ophiopogon japonicus root extract 1-5 parts by weight. Alternatively, The components of the natural preservative composition comprise Cinnamomum cassia bark extract 60-85 parts, Magnolia officinalis bark extract 8-20 parts, and Perilla frutescens leaf extract 8-20 parts by weight.

7. The natural preservative composition of claim 1, wherein The mass content of cinnamaldehyde in the Cinnamomum cassia bark extract is 5-15 wt%.

8. Process for the preparation of a natural preservative composition according to any one of claims 1 to 7, characterized in that, The method comprises the following steps: Mixing the components to obtain the natural preservative composition.

9. Use of the natural preservative composition according to any one of claims 1-7 in the preparation of a cosmetic or care product.

10. Use according to claim 9, characterized in that, The mass of the natural preservative composition added in the cosmetic is 0.08-0.5 wt% of the total mass of the cosmetic; or the mass of the natural preservative composition added in the care product is 0.08-0.5 wt% of the total mass of the care product.

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