Japanese cayratia herb skin antibacterial gel and preparation method thereof

Abstract

The invention discloses Japanese cayratia herb skin antibacterial gel and a preparation method thereof, and belongs to the field of traditional Chinese medicine preparations, and the Japanese cayratia herb skin antibacterial gel comprises carbomer, chitosan, glycerol, menthol, Japanese cayratia herb, rehmannia root, astragalus membranaceus, a bacteriostatic agent, a buffer solution and purified water. According to the Japanese cayratia herb skin antibacterial gel disclosed by the invention, the Japanese cayratia herb is matched with the radix rehmanniae and the radix astragali seu hedysari, so that the effects of clearing away heat and toxic materials, relieving swelling and pain, promoting blood circulation to remove blood stasis, relieving itching, resisting inflammation and inhibiting bacteria can be achieved, and the effects of local pain relieving and cooling can be achieved by adding the menthol; the active ingredients can be cross-linked, wrapped and fixed on the affected part, and in addition, the active ingredients have the antibacterial performance, so that the traditional Chinese medicine composition can better exert the antibacterial effect and promote the healing of the affected part. And the Japanese cayratia herb skin antibacterial gel is convenient and sanitary, and has higher comfort and stronger patient compliance compared with ointment.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine preparations, specifically relating to a skin antibacterial gel made from *Vaccaria segetalis* and its preparation method. Background Technology

[0002] Most existing antibacterial skin products are based on chemically synthesized drugs, which have problems such as strong irritation and poor transdermal absorption. Traditional Chinese medicine preparations have received widespread attention due to their natural properties and lower side effects. However, how to further optimize the formula to achieve higher antibacterial effects and a more comfortable user experience remains an urgent technical problem to be solved.

[0003] The medicinal use of *Rubus parvifolius* (also known as blackberry) has a long history. Tao Hongjing first recorded its medicinal effects: "Pound and apply to sores and swellings, snake and insect bites." It was first recorded in the *Tang Materia Medica*: "Blackberry, a creeping vine, with white leaves, sour and bitter in taste, cold in nature, and non-toxic. It is used to treat wind-heat swelling, erysipelas, and snake bites; pound and apply, and drink the juice." The *Compendium of Materia Medica* provides a detailed description: "Blackberry grows abundantly in ditches and trenches. Its vines are soft and angular, with one tendril per branch, green on the front and pale on the back; in July and August, it forms clusters of buds, with flowers as large as chestnuts, yellow; the fruit is as large as black nightshade seeds, green when unripe and purple when ripe, containing small seeds; its roots are white, the larger ones as large as a finger, one or two feet long, when pounded, they become very slippery and are used to cool the blood, detoxify, and promote urination; the root, pounded and taken with wine, can reduce boils and swellings." Additionally, the *Jiangsu Plant Medicinal Records* states that "soaking in hot water and fumigating the legs can relieve pain."

[0004] According to existing literature, *Rubus idaeus* possesses antibacterial, anti-inflammatory, analgesic, antiviral, antioxidant, heat-clearing, detoxifying, swelling-reducing, and nodule-dispersing effects. It can be used to treat surgical conditions such as carbuncles, boils, mumps, erysipelas, traumatic injuries, and snake and insect bites. Clinically, it is often formulated as an ointment to treat mastitis and perianal infections. *Rubus idaeus* ointment is a formulation of *Rubus idaeus* powder mixed with petroleum jelly, which can treat local redness, swelling, heat, and pain in sores at various stages, and can also treat lumps caused by certain genital diseases. However, as an ointment, it has a relatively thick and greasy texture.

[0005] Currently, there is a lack of skin antibacterial gel products and more diversified application scenarios for *Caulis Caulis Persicae*. *Caulis Caulis Persicae* is a natural Chinese medicinal herb, and the extract obtained contains a large number of active substances with low toxicity and unique pharmacological effects. Developing a modern topical gel formulation based on *Caulis Caulis Persicae* extract has become an important direction to enhance its clinical application value.

[0006] The conventional applications of chitosan and its derivatives in the pharmaceutical field are mostly concentrated on drug carriers, basic moisturizing in wound dressings, or simple antibacterial effects. Their functions are largely limited to physically blocking bacteria or slowly releasing loaded drugs, failing to fully realize their potential for synergistic effects with active ingredients in traditional Chinese medicine. Carbomer, as a traditional gel matrix, is conventionally used only to provide basic morphological support and drug dispersion carriers in gel formulations; its function is singular, and it has not formed functional synergies with other components. Menthol in conventional topical preparations is mostly used as a cooling agent to relieve immediate discomfort, and its synergistic antibacterial and analgesic effects with traditional Chinese medicine extracts and chitosan have not been found. In existing technologies, the combination of *Ulmus pumila*, *Rehmannia glutinosa*, and *Astragalus membranaceus* is mostly used for internal conditioning or simple efficacy superposition in traditional topical ointments, without deeply exploring the synergistic release and enhanced effects of the three active ingredients in gel formulations, nor achieving multi-dimensional functional synergy with components such as chitosan, carbomer, and menthol. Summary of the Invention

[0007] To address the aforementioned technical problems, this invention provides a skin antibacterial gel containing *Cymbidium goeringii*, its preparation method, and its application. Through innovative formulation and synergistic effects of its components, it overcomes the limitations of conventional single-component functions, achieving synergistic enhancement of multiple effects such as antibacterial, anti-inflammatory, and repair.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: The antibacterial skin gel made from *Corydalis yanhusuo* (a type of wild strawberry) in this invention is characterized in that the chitosan is one of carboxymethyl chitosan, chitosan lactate, hydroxypropyl chitosan, etc. Preferably, the chitosan is carboxymethyl chitosan. In conventional applications, chitosan is mainly used as a drug carrier or a basic antibacterial dressing, and its function is limited to physical barrier and slow drug release. In this invention, chitosan not only exerts its conventional effects of antibacterial activity, promoting wound healing, and inhibiting scarring, but also achieves two innovative functions: First, it forms a dual gel network with carbomer, which encapsulates and fixes the active ingredients of *Corydalis yanhusuo*, *Rehmannia glutinosa*, and *Astragalus membranaceus* through cross-linking, preventing ingredient loss and extending the retention time of effective ingredients at the affected area by 2-3 times; Second, its cationic properties form an electrostatic interaction with the active ingredients (such as flavonoids) in the extracts of traditional Chinese medicine, enhancing the skin affinity of the active ingredients, promoting transdermal absorption, and increasing the diameter of the antibacterial zone by 20%-30% compared with the control group without chitosan. This breaks through the conventional understanding that chitosan is only a "carrier" and becomes a dual-functional component of "synergist + carrier".

[0009] The antibacterial gel for skin of *Imperata cylindrica* in this invention is characterized in that the antibacterial agent is one or two of sodium methylparaben, sodium ethylparaben, and sodium propylparaben. Preferably, the antibacterial agent of this invention is sodium methylparaben and sodium propylparaben; more preferably, the mass ratio of sodium methylparaben to sodium propylparaben is 1.5~2.5. Conventional antibacterial agents only play a basic preservative role. In this invention, by using sodium methylparaben and sodium propylparaben in a specific mass ratio, not only is broad-spectrum antibacterial activity achieved (covering Gram-positive and Gram-negative bacteria), but it also forms a synergistic antibacterial system with traditional Chinese medicine extracts and chitosan: the traditional Chinese medicine extracts disrupt the bacterial cell membrane structure, the antibacterial agent inhibits bacterial metabolism, and chitosan blocks bacterial reproduction. The combination of these three increases the antibacterial efficiency by more than 40%, and reduces the concentration of a single antibacterial agent, reducing irritation. It achieves the dual effect of "preservative + synergistic antibacterial," breaking through the conventional understanding that antibacterial agents are only used for "pharmaceutical preservation."

[0010] The preparation method of the *Vaccaria segetalis* skin antibacterial gel of the present invention is characterized by comprising the following steps: (1) Dry and pulverize the medicinal materials *Ulva prostrata*, *Rehmannia glutinosa*, and *Astragalus membranaceus* mentioned in the formula to obtain powder; extract the powder with ethanol of a certain concentration to obtain extract; more preferably, the ratio of the powder to 70% ethanol is 1g:10-20g. Preferably, the extraction process is: reflux at 90-100℃ for 2-4 hours; concentrate the extract. (2) Dissolve the carbomer, chitosan and other ingredients in the specified amounts completely. (3) Add the prescribed amount of glycerin and extract solution and stir until well mixed; (4) Add an aqueous solution of sodium methylparaben and sodium propylparaben, an ethanolic solution of menthol, and finally add a buffer solution to adjust the pH. Add purified water to the total volume and stir until homogeneous.

[0011] According to literature reports, the whole plant of *Vaccaria segetalis* contains a variety of active ingredients, including flavonoids, volatile oils, and terpenoids. Extracting these active ingredients into organic solvents and dispersing them in a suitable gel matrix allows for direct skin contact, making them easier for the body to absorb and resulting in better effects.

[0012] The antibacterial skin gel of *Caulis Amaranthus* in this invention is characterized in that the antibacterial skin gel comprises carbomer 5-20 mg / g, *Caulis Amaranthus* 20-100 mg / g, Astragalus membranaceus 10-40 mg / g, Rehmannia glutinosa 10-20 mg / g, glycerin 30-60 mg / g, sodium methylparaben 0.5-1.5 mg / g, sodium propylparaben 0.25-0.10 mg / g, menthol 0.3-0.8 mg / g, and buffer solution.

[0013] The antibacterial gel for skin of *Vaccaria segetalis* in this invention is characterized in that the carbomer is a carbomer homopolymer type C.

[0014] The antibacterial gel for skin of *Cymbidium goeringii* in this invention is characterized in that the pH of the antibacterial gel for skin of *Cymbidium goeringii* is 4-8, preferably 6.5-7.5, and the buffer solution in this invention is a 10% citric acid solution and / or a triethanolamine solution, and the actual amount of buffer solution used is based on adjusting the pH of the antibacterial gel for skin of *Cymbidium goeringii* to 4-8.

[0015] The beneficial effects of this invention are as follows: (1) Novel extraction process for traditional Chinese medicine Supercritical fluid extraction technology is used to extract active ingredients from black clover, rehmannia root, and astragalus root, preserving their active ingredients while improving their utilization rate and reducing the damage to the active ingredients of traditional Chinese medicine caused by decoction methods.

[0016] (2) Diversified application scenarios The *Caulis Amaranthus* antibacterial skin gel of this invention, based on the heat-clearing, detoxifying, swelling-reducing, pain-relieving, and blood-activating effects of *Caulis Amaranthus* extract, is supplemented with two traditional Chinese medicinal herbs, *Rehmannia glutinosa* and *Astragalus membranaceus*, to enhance its swelling-reducing, pain-relieving, astringent, and hemostatic effects. Chitosan is added to achieve anti-inflammatory, antibacterial, wound-healing, and scar-inhibiting effects. Menthol is also added to provide local analgesia, cooling, and antipruritic effects. Its application scenarios are diversified, not only for postoperative repair but also for skin antibacterial care, daily bruises and sprains, and treatment of insect bites, meeting diverse market demands.

[0017] (3) Innovative gel matrix Currently, there is a lack of traditional Chinese medicine gel preparations made from herbal extracts and suitable matrices. This invention realizes the modern application of traditional Chinese medicine. Moreover, the antibacterial gel for skin of *Ulva scutellariae* described in this invention adopts a dual gel structure. The traditional carbomer gel matrix rapidly releases menthol, providing a cooling and soothing sensation, while the chitosan gel matrix can serve as a carrier for active ingredients, precisely releasing them to the affected area. The combination of the two is beneficial to improving the stability of active ingredients.

[0018] This invention is convenient and hygienic, more comfortable than ointments, non-greasy, has good spreadability, forms a film quickly, feels light and weightless on the skin, can promote wound healing, and achieves antibacterial effects on the skin. Detailed Implementation

[0019] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0020] Before further describing specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terminology used in the embodiments of the present invention is for describing specific embodiments and not for limiting the scope of protection of the present invention.

[0021] The present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all commercially available products in this technical field.

[0022] In this embodiment, the addition of *Vaccaria segetalis*, *Rehmannia glutinosa*, and *Astragalus membranaceus* is in the form of extract solutions, and the preparation method is as follows: (1) Crush the medicinal materials of *Vaccaria segetalis*, *Rehmannia glutinosa*, and *Astragalus membranaceus* to obtain powder; (2) Extract the powder with a certain concentration of ethanol to obtain the extract; (3) Concentrate the extract to obtain the final product.

[0023] More preferably, the ratio of the powder and 70% ethanol in step (2) is 1g:10-20g.

[0024] Preferably, the extraction in step (2) is performed by reflux at 90-100℃ for 2-4 hours.

[0025] It should be noted that although the raw materials are added in a certain order in this embodiment, the order does not have a substantial impact on the technical effect of the present invention. Therefore, the order of adding raw materials has no substantial limiting effect on the present invention.

[0026] Example 1: Blackberry Skin Antibacterial Gel The formula for the *Vaccaria segetalis* skin antibacterial gel is as follows: Carbomer 1.1%, purslane 6%, Astragalus 5%, Rehmannia glutinosa 4%, chitosan 0.5%, glycerol 5%, sodium methylparaben 0.04%, sodium propylparaben 0.04%, menthol 0.3% and buffer solution.

[0027] The preparation method is as follows: (1) First, the formula amount of *Caulis Myricae*, *Astragalus* and *Rehmannia* are pulverized and mixed with 70% ethanol solution at a ratio of 1:10. The mixture is refluxed at 90-100℃ for 2-4 hours. (2) First, the formula amount of carbomer is sprinkled into purified water. After it is completely dispersed and swollen, it is stirred and mixed until dissolved for 10-20 minutes. The formula amount of chitosan is added to purified water and stirred until completely dissolved. (3) Glycerin is added and stirred for 5-10 minutes. (4) *Caulis Myricae*, *Astragalus* and *Rehmannia* extracts are added. (5) Menthol is dissolved in an appropriate amount of purified water and added to it. (6) Antibacterial agent is added and mixed and stirred for 10-20 minutes. (7) Sodium citrate solution is added to adjust the pH. Finally, purified water is added to the required amount and stirred evenly to obtain the final product. The final pH is 4.3.

[0028] Example 2: Blackberry Skin Antibacterial Gel The formula for the *Vaccaria segetalis* skin antibacterial gel is as follows: Carbomer 1.3%, purslane 10%, Astragalus 8%, Rehmannia glutinosa 6%, chitosan 0.2%, glycerol 5%, sodium methylparaben 0.04%, sodium propylparaben 0.08%, menthol 0.6%, and buffer solution.

[0029] The preparation method is as follows: (1) First, the formula amount of *Caulis Myricae*, *Astragalus* and *Rehmannia* are pulverized and mixed with 70% ethanol solution at a ratio of 1:10. The mixture is refluxed at 90-100℃ for 2-4 hours. (2) The formula amount of chitosan is added to purified water and stirred until completely dissolved. The formula amount of carbomer is sprinkled into purified water and dispersed and swollen completely. The mixture is stirred until dissolved for 10-20 minutes. (3) Glycerin is added and stirred for 5-10 minutes. (4) Extracts of *Caulis Myricae*, *Astragalus* and *Rehmannia* are added. (5) Menthol is dissolved in an appropriate amount of purified water and added to the mixture. (6) Antibacterial agent is added and stirred for 10-20 minutes. (7) Sodium citrate solution is added to adjust the pH. Finally, purified water is added to the required amount and stirred evenly. The final pH is 4.8.

[0030] Example 3: Blackberry Skin Antibacterial Gel The formula for the *Vaccaria segetalis* skin antibacterial gel is as follows: Carbomer 0.8%, purslane 12%, Astragalus 10%, Rehmannia glutinosa 8%, chitosan 0.2%, glycerol 3%, sodium methylparaben 0.06%, sodium propylparaben 0.03%, menthol 0.4%, and buffer solution.

[0031] The preparation method is as follows: (1) First, the formula amount of *Caulis Myricae*, *Astragalus* and *Rehmannia* are pulverized and mixed with 70% ethanol solution at a ratio of 1:10. The mixture is refluxed at 90-100℃ for 2-4 hours. (2) The formula amount of chitosan is added to purified water and stirred until completely dissolved. The formula amount of carbomer is sprinkled into purified water and dispersed and swollen completely. The mixture is stirred until dissolved for 10-20 minutes. (3) Glycerin is added and stirred for 5-10 minutes. (4) Extracts of *Caulis Myricae*, *Astragalus* and *Rehmannia* are added. (5) Menthol is dissolved in an appropriate amount of purified water and added to the mixture. (6) Antibacterial agent is added and stirred for 10-20 minutes. (7) Sodium citrate solution is added to adjust the pH. Finally, purified water is added to the required amount and stirred evenly. The final pH is 6.6.

[0032] Example 4: Blackberry Skin Antibacterial Gel The formula for the *Vaccaria segetalis* skin antibacterial gel is as follows: Carbomer 0.6%, purslane 15%, Astragalus 12%, Rehmannia glutinosa 10%, chitosan 0.2%, glycerol 3%, sodium methylparaben 0.04%, sodium propylparaben 0.08%, menthol 0.6%, and buffer solution.

[0033] The preparation method is as follows: (1) First, the formula amount of *Caulis Myricae*, *Astragalus* and *Rehmannia* are pulverized and mixed with 70% ethanol solution at a ratio of 1:10. The mixture is refluxed at 90-100℃ for 2-4 hours. (2) The formula amount of chitosan is added to purified water and stirred until completely dissolved. The formula amount of carbomer is sprinkled into purified water and dispersed and swollen completely. The mixture is stirred until dissolved for 10-20 minutes. (3) Glycerin is added and stirred for 5-10 minutes. (4) *Caulis Myricae*, *Astragalus* and *Rehmannia* extracts are added. (5) Menthol is dissolved in an appropriate amount of purified water and added to the mixture. (6) Antibacterial agent is added and stirred for 10-20 minutes. (7) Triethanolamine is added to adjust the pH. Finally, purified water is added to the required amount and stirred evenly. The final pH is 6.6.

[0034] Experimental Example 1: Appearance Analysis of Traditional Chinese Medicine Gel As can be seen from the results in the table above, the various embodiments of the present invention exhibit good stability.

[0035] Test Example 2: Skin Irritation Test Three healthy volunteers applied the antibacterial skin gel prepared in Examples 3-5 directly to the skin on the back of their hands, and the contact sites were observed and recorded 12±2 hours after contact.

[0036] The results showed that no erythema or edema occurred at the contact sites of the three volunteers, indicating that the *Cymbidium goeringii* skin antibacterial gel of the present invention is mild and non-toxic, does not irritate the skin, and has good safety.

[0037] Experiment Example 3: Anti-swelling and Antipruritic Test Five volunteers who had been bitten by mosquitoes applied the antibacterial skin gel prepared in Examples 4-5 directly to the bite sites, and the contact sites were observed and recorded within 30-60 minutes.

[0038] The results showed that after applying the product to the mosquito bite sites of all five volunteers, the application site experienced a cooling sensation, and the itching was effectively suppressed, demonstrating a good anti-itch effect. In three of the volunteers, the bite marks essentially disappeared overnight after applying the product.

[0039] Test Example 4: In vitro antibacterial test (1) Test samples and experimental supplies The experimental drug was the gel from Examples 1-4 of this invention, prepared with distilled water to a 30% drug content solution, and then sterilized under high temperature and high pressure before use. Experimental supplies included: 90mm sterile plastic petri dishes, Oxford cups (8mm outer diameter), LB liquid medium, agar powder, TSA solid medium, and TSB liquid medium.

[0040] (2) Preparation of bacterial suspension The test bacteria were *Escherichia coli* (CMCC(B)44102) and *Staphylococcus aureus* (CMCC(B)26003). *E. coli* was resuspended in LB broth in a sterile laminar flow hood, and *Staphylococcus aureus* was resuspended in TSB broth. After resuscitation, the bacteria were streaked onto their respective solid media for isolation. Single colonies of the test bacteria were obtained after culturing on the corresponding solid media for 24 hours, and these single colonies were then cultured in broth. *E. coli* was incubated on LB broth at 37°C for 16-20 hours, and *Staphylococcus epidermidis* was incubated on TSB broth at 37°C for 16-20 hours. The bacterial cultures were then diluted to a bacterial suspension containing 10⁵ CFU / ml.

[0041] (3) Determination of inhibition zone This experiment used the Oxford cup method to measure the diameter of the inhibition zone of the tested bacteria and used it to determine the strength of the antibacterial activity of the test drug.

[0042] Procedure: All reagents and consumables required for the experiment were autoclaved at 121℃ for 20 minutes and set aside. Following aseptic technique, the pre-added bacterial suspension was poured onto plates, with three Oxford cups arranged in a triangle on each plate (three parallel controls). The sterilized solid culture medium was cooled to approximately 50℃, and 20 ml was dispensed. 1 ml (1×10⁵ CFU / ml) of bacterial suspension was added, mixed thoroughly, and poured into the plates containing the Oxford cups. After the plates solidified, the Oxford cups were removed with forceps, and the drug solution was dripped into the wells until they were 9 / 10 full. The plates were sealed with sealing film and incubated in a constant temperature and humidity incubator at 37℃ for 24–36 hours. The diameter of the inhibition zone was measured, and the average value was calculated.

[0043] (4) Results As shown in the table above, the herbal gel of the present invention has a moderately sensitive antibacterial effect against Staphylococcus aureus with an inhibition diameter between 10-15 mm; and a highly sensitive antibacterial effect against Escherichia coli with an inhibition diameter greater than 15 mm.

[0044] In summary, the antibacterial skin gel of the present invention, through the combination of extracts of *Vaccaria segetalis*, *Astragalus membranaceus*, and *Rehmannia glutinosa* with chitosan, menthol, etc., can achieve the effects of immediate cooling, antipruritic, antibacterial and anti-swelling.

[0045] The antibacterial gel for skin made from *Cymbidium goeringii* has good compatibility, good safety, and suitable viscosity. When applied to the target area, it can quickly form a film while moisturizing, and it is not prone to pilling, leaving the skin feeling light and comfortable.

[0046] It should be emphasized that the embodiments described in this invention are illustrative rather than limiting. Therefore, this invention includes, but is not limited to, the embodiments described in the specific implementation. Any other implementations derived by those skilled in the art based on the technical solutions of this invention are also within the scope of protection of this invention.

Claims

1. A skin bacteriostatic gel of Rubus corchorifolius, characterized by, Carbomer, chitosan, glycerol, menthol, extract of Smilax glabra, extract of Rehmannia glutinosa, extract of Astragalus membranaceus, bacteriostatic agent, buffer and purified water; the components synergize to break through the conventional function of single component and realize synergistic effect of bacteriostasis, anti-inflammation, analgesia and repair.

2. The Rubus corchorifolius skin bacteria-inhibiting gel of claim 1, wherein, The chitosan is one of carboxymethyl chitosan, chitosan lactate and hydroxypropyl chitosan, preferably carboxymethyl chitosan; the chitosan not only serves as a drug carrier and bacteriostatic component, but also forms a double gel network with carbomer to crosslink and wrap the active components of traditional Chinese medicine, thereby enhancing transdermal absorption and efficacy stability.

3. The Rubus corchorifolius skin bacteriostatic gel of claim 1, wherein, The bacteriostatic agent is one or two of sodium methylparaben, sodium ethylparaben and sodium propylparaben; preferably, the bacteriostatic agent is sodium methylparaben and sodium propylparaben, and further preferably, the mass ratio of sodium methylparaben to sodium propylparaben is 1.5-2.5; the bacteriostatic agent synergizes with the extract of traditional Chinese medicine and chitosan to reduce the use concentration of single bacteriostatic agent and enhance the broad-spectrum bacteriostasis and efficiency.

4. The Rubus corchorifolius skin bacteria-inhibiting gel of claim 1, wherein, The Smilax glabra skin bacteriostatic gel comprises 10-20 mg / g of carbomer, 20-100 mg / g of Smilax glabra extract, 10-40 mg / g of Astragalus membranaceus, 10-20 mg / g of Rehmannia glutinosa, 30-60 mg / g of glycerol, 0.5-1.5 mg / g of sodium methylparaben, 0.25-0.10 mg / g of sodium propylparaben, 0.3-0.8 mg / g of menthol and a buffer.

5. The Rubus corchorifolius skin bacteriostatic gel of claim 1, wherein, The carbomer is carbomer homopolymer type C which synergizes with chitosan to construct a double gel structure, thereby realizing the synergism of rapid release of menthol and slow release of the active components of traditional Chinese medicine.

6. The Rubus corchorifolius skin bacteria-inhibiting gel of claim 1, wherein, The pH of the Smilax glabra skin bacteriostatic gel is 4-8, preferably 6.0-7.5, and the buffer is 10% citric acid solution and / or triethanolamine solution.

7. The Rubus corchorifolius skin bacteriostatic gel of claim 1, wherein, The Smilax glabra, Rehmannia glutinosa and Astragalus membranaceus are alcohol extracts of whole plant of Smilax glabra, Rehmannia glutinosa and Astragalus membranaceus.

8. The method of making the Rubus corchorifolius skin bacteriostatic gel of any one of claims 1-7, characterized in that, The method comprises the following steps: (1) grinding Smilax glabra, Astragalus membranaceus and Rehmannia glutinosa to obtain powder; adding 10 times of alcohol of a certain concentration to the powder to obtain an extract; and concentrating the extract to obtain the extract of Smilax glabra, Astragalus membranaceus and Rehmannia glutinosa; (2) mixing the components of carbomer, chitosan, glycerol, sodium methylparaben, sodium propylparaben and menthol in purified water, adding the extract and finally adding a buffer to adjust the pH to obtain the Smilax glabra skin bacteriostatic gel.

9. Use of the Smilax glabra skin bacteriostatic gel of any one of claims 1-7 in the preparation of a skin bacteriostatic, anti-inflammatory, analgesic and wound repair product, which can be used for postoperative repair, daily skin bacteriostatic care, bruise and insect bite.

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