Botanical repellent for dendroctonus valerieus as well as preparation method and application of botanical repellent
By preparing a plant-derived repellent for the red turpentine beetle, and utilizing slow-release pouches containing components such as 1-octen-3-ol, cis-3-hexenol, and trans-2-hexenal, the problems of environmental pollution and insufficient preventative measures in the control of the red turpentine beetle were solved, achieving long-lasting repellency and ecological protection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-13
- Publication Date
- 2026-04-07
AI Technical Summary
Existing methods for controlling the red turpentine beetle have problems such as environmental pollution, accidental killing of non-target insects, and insufficient prevention. Chemical control results in pesticide residues, while biological control traps have limited coverage and are difficult to achieve comprehensive protection.
A plant-derived repellent for the red turpentine bark beetle, containing 1-octen-3-ol, cis-3-hexenol, and trans-2-hexenal, is prepared into slow-release bags for use in forest areas. It works by interfering with the pest's behavior, causing it to actively avoid the pest and preventing direct damage.
It achieves a long-lasting repellent effect, covering the main infestation period of the red turpentine beetle, reducing pesticide residues and labor costs, maintaining the ecological balance of the forest, and meeting the requirements of green forestry.
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Figure CN121795421A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of biological control, and particularly relates to a Dendroctonus valens plant-derived repellent and a preparation method and application thereof. BACKGROUND
[0002] Dendroctonus valens LeConte belongs to the family Scolytidae of the order Coleoptera, and is a major forestry quarantine pest in China. The pest mainly feeds on the host trees such as Pinus tabulaeformis at the base and root phloem of the host trees, and cuts off the nutrient transport of the host trees through circular boring, which leads to the rapid weakening and even death of the host. Dendroctonus valens has the characteristics of strong reproductive capacity, rapid spread, hidden harm and high mortality rate. Since it has entered China, it has posed a serious threat to the pine forest ecosystem in North China and Northwest China, and has become a key factor restricting the sustainable development of forestry ecological safety and health.
[0003] At present, the prevention and control of Dendroctonus valens mainly relies on two technical systems of chemical control and biological control. Chemical control can quickly reduce the pest population density in a short period of time through spraying or trunk injection of chemical pesticides, but long-term and large-scale use of chemical pesticides may lead to pesticide residues, environmental pollution, killing of natural enemies and destruction of forest ecological balance, which is contrary to the current green and sustainable forestry pest control philosophy.
[0004] In the biological control technology, the trapping and killing technology using information chemicals (such as aggregation pheromone) is widely used. This technology can simulate the release of pest pheromones to attract a large number of pests to a specific device for physical removal, and has a certain targeting and environmental friendliness. However, this technology has obvious limitations in practice. Its action mode is passive attraction followed by killing, and the coverage of the control is limited by the layout density and diffusion distance of the trap, making it difficult to achieve global protection in the forest area. In addition, in the early stage of prevention or monitoring stage when the pest population density is low, the control efficiency of the trap is relatively weak, and it is difficult to play an effective prevention and blocking role.
[0005] Repellents are a class of compounds that can interfere with the directional behavior of pests and make them actively avoid the protected object. The mechanism of action is mild, and it does not directly kill pests, has good environmental compatibility, is safe to non-target organisms, and is conducive to maintaining ecological balance. However, the research on high-efficiency and stable plant-derived repellents for Dendroctonus valens is still relatively scarce. Therefore, the existing prevention and control methods for Dendroctonus valens have problems such as environmental pollution, non-target killing, and insufficient prevention. SUMMARY
[0006] The present application aims to provide a plant-derived repellent for Dendroctonus valens, a preparation method and application thereof, which has a long pesticide residue persistence period and can cover the main damage period of Dendroctonus valens, thereby effectively preventing and treating Dendroctonus valens.
[0007] To solve the above technical problems, the technical scheme adopted by the present application is as follows: A plant-derived repellent for Dendroctonus valens comprises a solvent and an effective component, wherein the effective component is selected from one or more of 1-octen-3-ol, cis-3-hexenol and trans-2-hexenal.
[0008] Preferably, the solvent is selected from one of isopropyl alcohol or n-hexane.
[0009] Preferably, the effective component consists of cis-3-hexenol.
[0010] Preferably, the effective component consists of 1-octen-3-ol and trans-2-hexenal at a volume ratio of 1:1.
[0011] Preferably, the effective component consists of 1-octen-3-ol, cis-3-hexenol and trans-2-hexenal at a volume ratio of 1:1:1.
[0012] The present application also provides a preparation method of the plant-derived repellent for Dendroctonus valens, comprising the following steps: dissolving the effective component in the solvent, mixing uniformly, and obtaining the plant-derived repellent for Dendroctonus valens.
[0013] The present application also provides a Dendroctonus valens repellent slow-release bag, comprising: a breathable slow-release outer bag; an adsorption carrier arranged in the outer bag; and the plant-derived repellent for Dendroctonus valens as described, wherein the repellent is loaded on the adsorption carrier.
[0014] Preferably, the breathable slow-release outer bag is a black polyethylene bag with a size of 5cm*4cm; and the adsorption carrier is a polypropylene liquid-absorbing cotton with a size of 1.5cm*1.5cm*0.003cm.
[0015] The present application also provides an application of the plant-derived repellent for Dendroctonus valens in preventing and treating Dendroctonus valens.
[0016] The present application also provides a method for preventing and treating Dendroctonus valens, wherein any one of the repellent slow-release bags is arranged in the same trap together with a Dendroctonus valens attractant, and the trap is arranged in a Dendroctonus valens damage area; the trap is hung at a height of 30-50cm from the ground, and the distance between adjacent traps is 50-100m.
[0017] Compared with the prior art, the present application has the following advantages and technical effects: This invention discloses a plant-derived repellent for the red turpentine beetle, its preparation method, and its application. The core component of this repellent is a plant-derived volatile substance, and its mechanism of action is behavioral interference, causing the pest to actively avoid it, rather than directly killing it. This repellent does not produce pesticide residues and is safe for the natural enemies of the red turpentine beetle and other beneficial organisms in the forest area, thus maximizing the maintenance of forest ecological balance and fully meeting the requirements of green forestry and sustainable development.
[0018] This invention achieves slow and stable release of the active ingredient by loading the repellent into a slow-release bag. Outdoor trials show that the repellent has an effective release period of 2-2.5 months in field conditions, overcoming the short-lasting effect of traditional volatile compounds. It can cover the main infestation period of the red turpentine beetle, reducing the cost and manpower required for frequent repellent replacements.
[0019] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description
[0020] Figure 1 Figure showing the outdoor release rate of plant-derived repellents for the red turpentine beetle; Figure 2 The figure shows the normalized results of the average total number of red turpentine beetles trapped in Wenshui County in 2024. In the figure, ns represents P > 0.05. This means P ≤ 0.05. This means P ≤ 0.01; Figure 3 The graph shows the normalized results of the average total number of red grebested beetles trapped in Wenshui County in 2025. Figure 4 The figure shows the normalized results of the average total number of red turpentine beetles trapped in Xixian County in 2025. Figure 5 This is a summary chart of the total number of red turpentine beetles captured in three trapping experiments. Detailed Implementation
[0021] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.
[0022] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.
[0023] Source of experimental materials: In this invention, unless otherwise specified, all other test materials and instruments are conventional test materials in the field and can be purchased through commercial channels.
[0024] Example 1 Determination of the release effect of plant-derived repellents from the red turpentine beetle: Accurately measure 150 μL each of 1-octen-3-ol, cis-3-hexenol, and trans-2-hexenal. Dissolve each reagent separately in 850 μL of isopropanol. After vortexing to mix, transfer the solutions to polypropylene absorbent cotton (1.5 cm × 1.5 cm × 0.003 cm) inside a black polyethylene slow-release bag (5 cm × 4 cm). After the reagents are completely absorbed, seal each slow-release bag to prevent reagent evaporation from interfering with the experimental results.
[0025] The experiment consisted of three treatment groups, corresponding to the three repellents mentioned above. Each group had five replicates, with replicate samples numbered A1-A5 (1-octen-3-ol group), B1-B5 (cis-3-hexenol group), and C1-C5 (trans-2-hexenal group). The initial weight of each sealed sustained-release bag was weighed and accurately recorded. Subsequently, all sustained-release bags were placed in a pre-designated field experimental site, and the repellent release effect was dynamically measured for 7 days.
[0026] During the testing period, all sustained-release bags were collected daily at set times, and their weight changes were accurately measured and recorded. The average outdoor temperature during the experimental period was 20.1℃ ± 1.3℃. The results are shown in Table 1 and... Figure 1 As shown.
[0027] Table 1. Release rates of the three repellents during the testing period.
[0028] From Table 1 and Figure 1 The data shows that all three repellents maintained a stable release state throughout the testing period. Based on the 7-day release data, it is estimated that the effective release period of these three repellents can reach 2-2.5 months. Among them, the daily average release of cis-3-hexenol is higher than that of the other two reagents, which gives it a potential advantage in forest pest repellency applications.
[0029] Example 2 Field repellency effect determination of plant-derived repellent against red turpentine beetle: Field repellency experiment of red turpentine beetle: The repellent was prepared according to the method described in Example 1. The experiment consisted of three treatment groups, each with five replicates. The control group (CK) contained 15 mL of *Leptochloa crus-galli* attractant. Treatment group 1 contained 15 mL of *Leptochloa crus-galli* attractant, 150 μL of 1-octen-3-ol (dissolved in 850 μL isopropanol), 150 μL of cis-3-hexenol (dissolved in 850 μL isopropanol), and 150 μL of trans-2-hexenal (dissolved in 850 μL isopropanol). Treatment group 2 contained 15 mL of *Leptochloa crus-galli* attractant and 150 μL of cis-3-hexenol (dissolved in 850 μL isopropanol). A funnel-shaped *Leptochloa crus-galli* trap (Pheromone, Hangzhou) was used. After assembly strictly following the instructions, the reagent components for each treatment group were placed into slow-release bags and placed in a suitable container. The traps were placed in the second funnel from top to bottom. All traps were then suspended in a Pinus tabuliformis forest in Wenshui County, Lüliang City, Shanxi Province, where the Red Turtle Beetle was severely infested. The traps were suspended at a height of 50 cm above the ground, and the distance between adjacent traps was set at 50 m to avoid interference between treatment groups. Based on relevant data regarding the flight period of adult Red Turtle Beetles, the experiment was scheduled to run from May 13, 2024 to August 11, 2024. During the experiment, the slow-release bags in each trap were replaced on July 17, 2024, to ensure the stability of the experimental conditions.
[0030] Data processing and analysis: The data from the field trapping experiment were normalized using Microsoft Excel software. The normalization calculation was performed using the following formula: ; in, and These are the maximum and minimum values in the data, respectively. The trapping amount is normalized.
[0031] The processed data were analyzed using IBM SPSS Statistics 26 with one-way ANOVA and Tukey's method for multiple comparisons. Bar charts were then generated using GraphPad Prism 8.0 (GraphPad, USA). The results are shown below. Figure 2 As shown.
[0032] Depend on Figure 2 It can be seen that there is a highly significant difference in the number of traps between the control group (CK) and treatment group 1 (P<0.01), and a significant difference in the number of traps between the control group (CK) and treatment group 2 (P<0.05), proving that the repellent component has significant repellent activity against red turpentine beetles, and that the repellent effect of treatment group 1 is better than that of treatment group 2.
[0033] Example 3 Optimized field repellency effect of plant-derived repellent against red turpentine beetle: To further verify the actual repellency effect of the aforementioned repellent in the field, the field repellency experiment was repeated in 2025, with some experimental operation details optimized. A new treatment group (Treat3) was added, containing 15 mL of *Pinus tabuliformis* attractant, 150 μL of 1-octen-3-ol (dissolved in 850 μL of isopropanol), and 150 μL of trans-2-hexenal (dissolved in 850 μL of isopropanol). The experimental sites were located in severely infested *Pinus tabuliformis* forests in Wenshui County, Lüliang City, Shanxi Province, and Xi County, Linfen City, Shanxi Province. The experimental periods for the two locations were set from April 23, 2025 to June 6, 2025, and from April 30, 2025 to June 10, 2025, respectively. The trap hanging method, field survey procedure, and experimental data processing method were strictly performed according to the conditions described in Example 2 of this application. The results are as follows... Figures 3-4 As shown.
[0034] Depend on Figures 3-4 It can be seen that there is a significant difference in the number of traps between the control group (CK) and treatment group 1 (P<0.05); although the number of traps in the other treatment groups showed a downward trend compared with the control group, the experimental error was large due to the interference of complex environmental factors in the wild and did not reach the level of statistical significance.
[0035] To clarify the actual application effect of the repellent, a summary analysis of three field trapping experiments over two years was conducted. The total number of adult red turpentine beetles trapped in the control group and each treatment group was compared. The results are as follows: Figure 5 As shown.
[0036] Depend on Figure 5 It can be seen that in the field trapping experiments in Wenshui County in 2024, 2025, and Xi County in 2025, the total number of *Lysimachia rubiginosa* trapped in the control group was 748, 174, and 322, respectively, which was much larger than the total number trapped in the treatment group. This proves that the repellent component has good repellent activity against *Lysimachia rubiginosa*.
[0037] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.
Claims
1. A plant-derived repellent for the red turpentine beetle, characterized in that, It contains a solvent and an active ingredient, said active ingredient being selected from one or more of 1-octen-3-ol, cis-3-hexenol, and trans-2-hexenal.
2. The repellent according to claim 1, characterized in that, The solvent is selected from isopropanol or n-hexane.
3. The repellent according to claim 1 or 2, characterized in that, The active ingredient consists of cis-3-hexenol.
4. The repellent according to claim 1 or 2, characterized in that, The active ingredient is composed of 1-octen-3-ol and trans-2-hexenal in a volume ratio of 1:
1.
5. The repellent according to claim 1 or 2, characterized in that, The active ingredient is composed of 1-octen-3-ol, cis-3-hexenol and trans-2-hexenal in a volume ratio of 1:1:
1.
6. A method for preparing a plant-derived repellent for *Ceratophyllum demersum* as described in claim 1, characterized in that, Includes the following steps: The active ingredient was dissolved in a solvent and mixed evenly to obtain a plant-derived repellent for the red turpentine beetle.
7. A slow-release bag for repelling red liptinous beetles, characterized in that, include: Breathable, slow-release outer bag; An adsorption carrier disposed inside the outer bag; And a plant-derived repellent for *Ceratophyllum demersum* as described in any one of claims 1-5, wherein the repellent is loaded onto the adsorbent carrier.
8. The repellent and slow-release bag according to claim 7, characterized in that, The breathable slow-release outer bag is a black polyethylene bag with dimensions of 5cm × 4cm; the adsorption carrier is polypropylene absorbent cotton with dimensions of 1.5cm × 1.5cm × 0.003cm.
9. The application of the plant-derived repellent for red turpentine beetle as described in claim 1 in the control of red turpentine beetle.
10. A method for controlling the red turpentine beetle, characterized in that, The repellent slow-release bag according to any one of claims 7-8 is placed together with the red turpentine beetle attractant in the same trap, and the trap is placed in the area infested by the red turpentine beetle; the trap is suspended at a height of 30-50cm above the ground, and the distance between adjacent traps is 50-100m.