Barrier repair type anti-inflammatory hydrogel as well as preparation method and application thereof

Abstract

The invention discloses barrier repair type anti-inflammatory hydrogel as well as a preparation method and application thereof, and relates to the technical field of skin repair and inflammation regulation, and the method comprises the following steps: dissolving Cu < 2 + >-containing copper salt in sterile deionized water to obtain a Cu < 2 + > solution, heating, adding xanthan gum, and uniformly mixing to obtain initial hydrogel; sodium hyaluronate, butanediol, glycerol, ceramide, nicotinamide, panthenol and allantoin are added into the initial hydrogel, then the pH value of the obtained system is adjusted to be neutral or weakly acidic, the barrier repair type anti-inflammatory hydrogel is obtained, the ratio of the mass of the copper salt to the hydrogel is (0.01-0.1) g: 100 mL, the action time of active substances is effectively prolonged by stably releasing copper ions, and the barrier repair type anti-inflammatory hydrogel is obtained. The composition can regulate the expression level of local inflammatory factors, improve the problem that the skin is in a high inflammatory state for a long time, improve the local hydration of the skin, and provide a favorable repair environment for a damaged barrier so as to promote the recovery of the structure and function of the skin.

Description

Technical Field

[0001] This invention relates to the field of skin repair and inflammation regulation technology, specifically to a barrier repair type anti-inflammatory hydrogel, its preparation method, and its application. Background Technology

[0002] Seborrheic dermatitis is a common chronic relapsing inflammatory skin disease, typically manifesting as erythema, desquamation, abnormal sebum secretion, and persistent itching. Its pathogenesis involves multiple factors, including skin barrier damage, sebum metabolism disorder, microecological imbalance, and abnormal inflammatory responses. Among these, the abnormal expression of inflammatory factors plays a crucial role in the development and symptom maintenance of seborrheic dermatitis. When the skin barrier function is impaired, inflammatory-related cytokines (such as IL-1β, TNF-α, and IL-8) will be persistently elevated locally, further exacerbating barrier damage and creating a vicious cycle of inflammation leading to barrier injury, thus prolonging the course of the disease and causing recurrent symptoms.

[0003] Current treatments for seborrheic dermatitis primarily include anti-inflammatory drugs, antifungal medications, and keratinocyte-derived skin care products. While these products have some effect in controlling symptoms or inhibiting microbial proliferation, they still have significant limitations. For example, long-term use of anti-inflammatory drugs may lead to skin dependence or irritation; antifungal drugs, although able to inhibit Malassezia furfur, cannot effectively regulate persistent skin damage caused by inflammatory imbalance; and keratinocyte-derived skin care products are mostly routine moisturizing products, mainly focusing on providing surface hydration and failing to deeply improve barrier structure and microenvironment homeostasis. Therefore, in clinical and nursing settings, many patients with seborrheic dermatitis still face problems such as long recovery periods, difficulty in completely alleviating inflammation, and unsatisfactory restoration of barrier function.

[0004] In recent years, the potential of metal ions in regulating skin inflammatory responses has gradually attracted attention. Studies have shown that copper ions (Cu...) 2+ Copper ions not only possess antibacterial and antioxidant bioactivities, but can also regulate the expression of inflammatory factors by influencing inflammatory signaling pathways, thereby alleviating local skin inflammation. Furthermore, copper ions promote fibroblast activity and collagen production, contributing to the reconstruction of damaged skin barriers. However, direct topical application of copper ions presents challenges such as irritation, difficulty in maintaining effective duration, and uneven distribution in local tissues, limiting their application in inflammatory skin diseases.

[0005] Existing copper ion hydrogels directly mix copper ions into the hydrogel network, resulting in a burst release due to physical mixing. However, insufficient copper ion concentration in the later stages prevents stable release. Furthermore, their reliance on high-concentration copper ions acting directly on the skin surface easily triggers cellular oxidative stress. Simultaneously, the mechanical strength of existing hydrogels rapidly declines with ion release, leading to premature gel disintegration and an inability to provide a physical scaffold for epidermal cells. Therefore, current treatments suffer from unsustainable anti-inflammatory effects, difficulty in modulating deep inflammatory mechanisms, and insufficient support for barrier reconstruction. There is an urgent need to develop a topical carrier capable of stably loading and sustaining the release of copper ions, enabling gentle and continuous regulation of inflammatory factors on the skin surface while providing a favorable microenvironment for barrier repair. Summary of the Invention

[0006] To address the problems existing in the prior art, this invention provides a barrier-repairing anti-inflammatory hydrogel, its preparation method, and its application. This hydrogel, as a material with excellent moisturizing, film-forming, and biocompatibility, effectively prolongs the action time of active substances by stably releasing copper ions, regulates the expression level of local inflammatory factors, improves the problem of long-term high inflammation of the skin, improves local skin hydration, and provides a favorable repair environment for the damaged barrier to promote the recovery of skin structure and function. This provides a new technical path for achieving synergistic treatment of inflammation regulation and barrier repair.

[0007] This invention is achieved through the following technical solution: A barrier-repairing anti-inflammatory hydrogel, the raw materials of which include Cu 2+ The solution contains xanthan gum, sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol, and allantoin, among which Cu... 2+ In the solution, the ratio of the mass of the corresponding copper salt to the hydrogel is (0.01–0.1) g: 100 mL.

[0008] Preferably, the ratio of xanthan gum to hydrogel is (0.01-10) g: 100 mL, and the ratio of sodium hyaluronate to hydrogel is (0.01-1) g: 100 mL.

[0009] Preferably, the Cu 2+ One or more of copper sulfate, copper gluconate, copper citrate, copper carbonate, and copper lactate.

[0010] Preferably, the ratio of butanediol to hydrogel is (0.1-10) g: 100 mL, and the ratio of glycerol to hydrogel is (0.01-10) g: 100 mL.

[0011] Preferably, the ratio of ceramide to hydrogel is (0.01-1) g: 100 mL, the ratio of nicotinamide to hydrogel is (0.1-5) g: 100 mL, the ratio of panthenol to hydrogel is (0.1-10) g: 100 mL, and the ratio of allantoin to hydrogel is (0.01-1) g: 100 mL.

[0012] A method for preparing a barrier-repairing anti-inflammatory hydrogel includes the following steps: S1, containing Cu 2+ Copper salts are dissolved in sterile deionized water to obtain Cu. 2+ The solution was heated, and then xanthan gum was added and mixed well to obtain the initial hydrogel. S2, add sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol and allantoin to the initial hydrogel, and then adjust the pH of the resulting system to neutral or weakly acidic to obtain a barrier repair anti-inflammatory hydrogel.

[0013] Preferably, S1 will use Cu 2+ Heat the solution to 40-50℃, then add xanthan gum and mix well.

[0014] Preferably, S2 adjusts the pH of the resulting system to 5.8–7.2 to obtain a barrier-repairing anti-inflammatory hydrogel.

[0015] A barrier-repairing anti-inflammatory hydrogel is prepared by any of the above-described preparation methods.

[0016] Application of a barrier-repairing anti-inflammatory hydrogel in the preparation of topical formulations for seborrheic dermatitis and related skin barrier damage.

[0017] Compared with the prior art, the present invention has the following beneficial technical effects: This invention discloses a barrier-repairing anti-inflammatory hydrogel, using divalent copper ions as the core functional component. A stable hydrogel matrix is ​​constructed by combining xanthan gum and sodium hyaluronate, and synergistically introducing skin barrier repair auxiliary components such as butylene glycol, glycerin, ceramide, niacinamide, panthenol, and allantoin. This forms a composite system with both anti-inflammatory regulation and barrier reconstruction functions. Divalent copper ions initially exist as soluble copper salts, and then, by stably loading bioactive copper ions into a three-dimensional gel network, they can be continuously released on the skin surface. This constructs a multifunctional gel system with moisturizing film-forming, inflammation regulation, and barrier enhancement capabilities. This creates a stable microenvironment conducive to tissue regeneration during skin repair, thereby regulating the inflammatory response on the skin surface (balancing the expression of pro-inflammatory cytokines and immunomodulatory factors) and enhancing barrier repair capabilities, thus improving inflammation-related skin barrier function. On one hand, Cu... 2+The sustained-release effect of this hydrogel can inhibit the abnormal expression of pro-inflammatory factors, reducing erythema, desquamation, and itching caused by inflammation. Simultaneously, a mild concentration of copper ions can promote fibroblast activity and increase the production of skin barrier-related proteins and extracellular matrix. Furthermore, the polymer matrix in the gel can form a flexible protective film, reducing further damage to damaged skin from external stimuli and providing a suitable moist environment to accelerate barrier repair, exhibiting anti-Malassezia furfur activity. Experiments have shown that this type of hydrogel has excellent biocompatibility and good skin adhesion, significantly reducing inflammation levels and accelerating barrier structure recovery, making it suitable for skin problems such as seborrheic dermatitis, which are mainly characterized by inflammation and barrier damage. This hydrogel exhibits good skin compatibility under weakly acidic to neutral conditions, effectively improving the inflammatory microenvironment associated with seborrheic dermatitis by regulating the expression of the pro-inflammatory cytokine IL-6 and maintaining or promoting the physiological level of the immunomodulatory cytokine IL-2; simultaneously, it accelerates the repair process of the damaged skin barrier by promoting the stability of the stratum corneum lipid structure and the restoration of skin hydration.

[0018] This invention further provides a method for preparing a barrier-repairing anti-inflammatory hydrogel, comprising: using Cu-containing... 2+ The copper salt is dissolved in sterile water; then xanthan gum is added, and under constant temperature stirring conditions, it is allowed to fully swell, allowing copper ions to bind into the gel network structure. Sodium hyaluronate and skin barrier repair auxiliary ingredients butylene glycol, glycerin, ceramide, niacinamide, panthenol, and allantoin are then added, and stirring continues to homogenize the system, forming a gel structure in which copper ions remain stably distributed. By utilizing the electrostatic attraction between the anions in xanthan gum and sodium hyaluronate and the cations in the copper salt, a dense and uniform network structure is formed in the gel, thereby controlling the release rate of copper ions. This cross-linking process not only improves the mechanical strength and structural stability of the hydrogel, but also allows for adjustment of the gel's swelling capacity and adhesion as needed, ensuring that the hydrogel of this invention has good durability and a comfortable application experience on the skin surface, thus obtaining a functional hydrogel with purer ingredients and more stable performance. This preparation process is mild and safe, does not require high-energy reaction conditions, does not produce harmful byproducts to the skin, has controllable costs, is easy to scale up for industrial production, and is suitable for long-term promotion.

[0019] The hydrogel of this invention is suitable for daily care of seborrheic dermatitis and related skin barrier dysfunction in the form of a topical formulation. It has advantages such as safe ingredients, mild effects, and suitability for long-term use, and possesses good prospects for industrialization and commercial application. The hydrogel of this invention can improve persistent inflammatory states and promote the restoration of skin barrier structure and microenvironment by influencing the production and release of local inflammatory mediators. It is suitable for the adjunctive repair of skin lesions on the surface of human or animal skin characterized by inflammatory responses and barrier damage, primarily associated with seborrheic dermatitis. Attached Figure Description

[0020] Figure 1 This is a photograph of the copper ion hydrogel obtained in Example 1 of the present invention after it was injected onto the surface of a culture dish using a 5mL syringe. Figure 2a The rheological properties (strain) diagrams of the hydrogels obtained in Examples 1 and 2 of this invention are shown, where the lower left column is G' and the right column is G''. Figure 2b The rheological properties (stress) diagrams of the hydrogels obtained in Examples 1 and 2 of this invention are shown, where the lower left column is G' and the right column is G''. Figure 3 This is a diagram showing the adhesion and moisturizing effect of the hydrogel obtained in Example 1 of the present invention on the skin surface; Figure 4 The graph shows the changes in the treatment of Malassezia furfur seborrheic dermatitis by the hydrogels obtained in Examples 1 and 2 of this invention, the control group, and ketoconazole. Figure 5 This is a comparative graph showing the changes in the expression levels of inflammatory factors after applying the hydrogel of this invention. Detailed Implementation The present invention will be further described in detail below with reference to specific embodiments. These descriptions are for explanation purposes only and are not intended to limit the scope of the invention.

[0021] In a first aspect, the present invention discloses a copper ion-based barrier-repairing anti-inflammatory hydrogel, the raw material of which includes Cu 2+ Copper salts, xanthan gum, sodium hyaluronate, butylene glycol, glycerin, ceramides, nicotinamide, panthenol, and allantoin. Among them, Cu... 2 + Copper salts are derived from one or more of copper sulfate, copper gluconate, copper citrate, copper carbonate, and copper lactate.

[0022] By weight percentage, each 100 mL of copper ion hydrogel contains 0.01%–0.1% copper salt, 0.01%–1% sodium hyaluronate, 0.01%–10% xanthan gum, 0.1%–10% butylene glycol, 0.01%–10% glycerol, 0.01%–1% ceramide, 0.1%–5% nicotinamide, 0.1%–10% panthenol, and 0.01%–1% allantoin. The percentages are calculated by dividing the mass (g) of each ingredient by the total volume (mL) of the hydrogel.

[0023] In a second aspect, the present invention discloses a method for preparing the above-mentioned hydrogel, comprising the following steps: dissolving copper salt in sterile deionized water to obtain a copper salt solution; heating the solution in a water bath to 40-50°C and then adding xanthan gum to the copper salt solution and mixing to obtain an initial hydrogel; and sequentially adding sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol, and allantoin, and adjusting the pH of the system to neutral or weakly acidic (5.8-7.2) to suit the physiological environment of human or animal skin surface to obtain the hydrogel.

[0024] Example 1 A copper ion-based barrier-repairing anti-inflammatory hydrogel is composed of the following raw materials: 0.08% copper sulfate, 2% xanthan gum, 0.5% sodium hyaluronate, 3% butylene glycol, 6% glycerin, 1% ceramide, 5% nicotinamide, 3% panthenol, and 0.1% allantoin. A method for preparing a copper ion-based barrier-repairing anti-inflammatory hydrogel includes the following steps: S1. Dissolve copper sulfate in sterile deionized water to obtain a copper sulfate solution; S2. Heat the copper sulfate solution to 40 degrees Celsius in a water bath, then add xanthan gum to the solution and mix well to obtain the initial hydrogel. S3. Add sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol, and allantoin to the initial hydrogel in sequence, and adjust the pH value to 7 to obtain a copper ion-based barrier repair anti-inflammatory hydrogel. Example 2 A copper ion-based barrier-repairing anti-inflammatory hydrogel is composed of the following raw materials: 0.05% copper sulfate, 4% xanthan gum, 0.1% sodium hyaluronate, 5% butylene glycol, 10% glycerin, 0.5% ceramide, 2.5% nicotinamide, 1% panthenol, and 0.05% allantoin. A method for preparing a copper ion-based barrier-repairing anti-inflammatory hydrogel, the steps of which are as described in Example 1.

[0025] Example 3 A copper ion-based barrier-repairing anti-inflammatory hydrogel is composed of the following raw materials: 0.02% copper sulfate, 8% xanthan gum, 1% sodium hyaluronate, 0.5% butylene glycol, 0.1% glycerin, 0.2% ceramide, 0.5% nicotinamide, 5% panthenol, and 1% allantoin. A method for preparing a copper ion-based barrier-repairing anti-inflammatory hydrogel, the steps of which are as described in Example 1.

[0026] Example 4 A copper ion-based barrier-repairing anti-inflammatory hydrogel is composed of the following raw materials: 0.03% copper sulfate, 4% xanthan gum, 0.4% sodium hyaluronate, 9% butylene glycol, 0.5% glycerin, 0.8% ceramide, 0.2% nicotinamide, 9% panthenol, and 0.3% allantoin. A method for preparing a copper ion-based barrier-repairing anti-inflammatory hydrogel, the steps of which are as described in Example 1.

[0027] Example 5 A copper ion-based barrier-repairing anti-inflammatory hydrogel is composed of the following raw materials: 0.1% copper sulfate, 0.6% xanthan gum, 0.7% sodium hyaluronate, 3.5% butylene glycol, 10% glycerin, 0.4% ceramide, 3% nicotinamide, 0.8% panthenol, and 0.2% allantoin. A method for preparing a copper ion-based barrier-repairing anti-inflammatory hydrogel, the steps of which are as described in Example 1.

[0028] Performance testing 1. Morphological observation of copper ion hydrogels: Example 1 was extruded onto the surface of a culture dish using a 5 mL syringe with a needle. The morphological retention of the extruded structure under unconstrained conditions was observed and analyzed, including its shape fidelity, structural uniformity, continuity, and adhesion strength to the culture dish.

[0029] Result: As Figure 1 As shown, copper ion hydrogels exhibit significant shear-thinning properties, allowing for smooth injection through fine needles and immediate recovery of mechanical strength after extrusion, maintaining their pre-defined shape. This combination of injectability and rapid self-support makes them ideal for use as in-situ molded hydrogel dressings.

[0030] 2. Determination of the rheological properties of hydrogels: The rheological properties of hydrogel samples were determined using a rotational rheometer within the frequency range of 0–100 Hz. Data on the storage modulus (G') and loss modulus (G'') as a function of frequency were recorded to determine the linear apparent elastic range (LVR) of the samples. All subsequent rheological measurements were performed within the defined LVR range. Strain-stress scans were performed within the range of 0.1–100%.

[0031] Result: As Figure 2a and Figure 2bAs shown, the yield stress and yield strain results indicate that in Examples 1 and 2, the initial value of G′ is greater than that of G″, indicating that it mainly exhibits elastic behavior. With increasing stress and strain, G″ increases while G′ decreases, and the two tend to intersect, marking a shift from elastic to viscous dominance. This pseudoplastic characteristic suggests that under shear stress, the hydrogel transitions to a fluid state, thereby promoting diffusion. This indicates that the hydrogel has a greater energy storage capacity within a small deformation range, reflecting a higher elastic modulus. The copper ion hydrogel maintains continuity and support under larger strains, indicating better ductility and flexibility. These properties make copper ion hydrogels suitable for wounds requiring continuous coverage or frequent deformation. In summary, the rheological results are consistent with the hydrogel injection experiment results. The results show that the copper ion hydrogel forms an elastic-dominant structure, demonstrating good injectability and deep penetration capabilities, enabling seamless filling of irregular wound defects. The material's ability to maintain continuity under overall strain reflects its excellent ductility and flexibility. This mechanical compliance allows it to adapt to dynamic deformations such as joint bending or skin stretching, preventing dressing breakage or detachment. Copper ion hydrogels exhibit superior properties as wound dressings, cleverly balancing high elasticity (protection) under static conditions with fluid behavior (ease of use) under dynamic conditions, perfectly meeting the clinical need for wound dressings to balance ease of use and long-term structural integrity.

[0032] 3. Analyze the adhesion and moisturizing effects of hydrogels on the skin surface: Using a 5 mL sterile syringe, draw up the sample from Example 1. Then, hold the syringe needle perpendicular to the skin surface of the back of the hand about 1 cm away, and slowly push the plunger until a complete drop of hydrogel (about 5 mm in diameter) is dispensed, allowing it to settle and adhere to the skin. Once the droplet has stabilized, use the pad of your index finger to spread the droplet evenly in a uniform, unidirectional motion.

[0033] result: Figure 3 The fact that the copper ion hydrogel maintains its original shape when dropped onto the skin indicates that its cross-linked network is dense and possesses a certain structural strength. The polymer chains in the hydrogel can form intermolecular forces with the stratum corneum of the skin, demonstrating good affinity for the skin. It can be evenly spread to form a continuous, tightly covering film on the skin surface, which is the physical basis for moisturizing and promoting penetration. This proves that the copper ion hydrogel can form a skin-adhering film with good extensibility and adhesion.

[0034] 4. Evaluate the therapeutic effect of hydrogel on seborrheic dermatitis caused by Malassezia furfur: Model Establishment: Guinea pigs were anesthetized by intraperitoneal injection of a mixture of ketamine (50 mg / mL) and xylazine (8 mg / mL) in a 1:1 volume ratio, with a total volume of 0.1 mL. Each guinea pig was anesthetized, and the hair on its back was shaved with a pet-specific shaving tool. Any remaining hair was removed with depilatory cream. The back was then wiped with cotton balls soaked in 75% alcohol. After drying, a 4 cm × 4 cm area was marked with a ruler. Sandpaper was used to rub the guinea pig's back until it bled but did not bleed. 200 μL of Malassezia furfur suspension was evenly applied to the hairless area on the back once daily for 7 consecutive days, and the model establishment was monitored. After successful modeling, guinea pigs were treated once daily with Example 1 and Example 2, respectively, while the control group was treated with physiological saline, for 14 consecutive days.

[0035] Result: As Figure 4 The diagram shows the actual condition of the wound during treatment. In the wound healing diagram, the blue, yellow, and purple areas represent the wound area on days 1, 7, and 14, respectively. Significant differences were observed in wound healing after applying copper ion hydrogel. During the wound healing process, the hydrogel-treated group in Example 2 showed the fastest closure speed, significantly better than the control group and the ketoconazole group. Day 0: All three groups of guinea pigs were expected to show typical dermatitis manifestations such as erythema, scaling, and mild infiltration in the modeled areas. There was no statistically significant difference in skin lesion scores among the groups. Day 7: The skin lesions in the control group showed no significant improvement and even slightly worsened due to persistent infection. Erythema and scaling would persist. Copper ion hydrogel group: Showed preliminary treatment effect. The severity of skin lesions showed a decreasing trend compared to day 0. Erythema slightly subsided, and scaling significantly decreased. Day 14: The skin lesions in the control group continued to expand, with a significant increase in erythema. Due to the itching caused by seborrheic dermatitis, the guinea pigs scratched, causing skin damage and even bleeding, remaining the most severe among the three groups. Copper ion hydrogel group: Skin lesions continued to improve, achieving the best therapeutic effect. Most skin lesions were nearly completely healed, with only mild skin redness remaining. Erythema and scaling disappeared, and new hair grew at the model site, indicating that the dermatitis had almost completely recovered. These results demonstrate that copper ion hydrogel has good healing-promoting properties.

[0036] 5. Verify the anti-inflammatory ability of copper ion hydrogel: On day 14 of treatment, blood samples were collected from guinea pigs, and guinea pig serum was obtained by centrifugation. The levels of pro-inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-2 (IL-2), in the guinea pig serum were measured using an enzyme-linked immunosorbent assay (ELISA) kit.

[0037] The results are as follows Figure 5 : IL-6 (a pro-inflammatory cytokine) is a key cytokine that plays a pleiotropic role in skin wound healing, and the dynamic balance of its expression is a core factor determining the healing outcome.

[0038] In the infected model group (inoculated with Malassezia furfur, shown in the figure), the IL-6 level was significantly increased to 165.85 pg / mg, which was highly significant compared with the uninfected model group (not inoculated with Malassezia furfur, shown in the figure) (80.75 pg / mg), indicating that Malassezia furfur infection can induce a strong inflammatory response.

[0039] Copper ion hydrogel groups: In Example 1, the IL-6 level decreased to 49.88 pg / mg, and in Example 2, it decreased to 35.62 pg / mg, both significantly lower than the infection model group. Compared with the infection group, the hydrogel could downregulate IL-6 by 115.97–130.23 pg / mg. The results indicate that both copper ion hydrogels can effectively inhibit excessive IL-6 secretion.

[0040] IL-2 (immunomodulatory cytokine) plays a dual role in skin healing by differentially regulating the balance between effector and regulatory T lymphocytes: in the early stage, it mainly controls inflammation by maintaining the function of regulatory T cells, while in the middle and late stages, the immune response it drives profoundly affects the quality of tissue repair and scar outcome.

[0041] In the infection model group, the IL-2 level was significantly increased to 84.77 pg / mg, which was highly significant compared with the non-infection model group (26.28 pg / mg), indicating that infection can activate immune cells and promote IL-2 release.

[0042] Copper ion hydrogel group: The IL-2 content in the first case group decreased to 7.96 pg / mg, and in the second case group it decreased to 6.83 pg / mg, both significantly lower than that in the infection model group. Compared with the infection group, IL-2 was downregulated by 76.81 to 77.94 pg / mg, and there was no significant difference between the groups.

[0043] This indicates that copper ion hydrogels can effectively downregulate the abnormal increase in IL-2, thereby alleviating the state of excessive immune activation.

[0044] In summary, Malassezia furfur infection significantly upregulates the expression of IL-6 and IL-2, triggering inflammatory responses and immune activation. Both Example 1 and Example 2 effectively inhibited the excessive secretion of these two inflammatory factors. The levels of pro-inflammatory factors in the example groups were significantly different from those in the control group. These results indicate that Cu-loaded... 2+ The subsequent hydrogel exhibited good anti-inflammatory effects. Overall, the copper ion hydrogel, by downregulating pro-inflammatory cytokines and alleviating excessive inflammatory responses, promoted wound healing and has great potential for application in the inflammatory intervention of seborrheic dermatitis.

Claims

1. A barrier-repairing anti-inflammatory hydrogel, characterized in that, Raw materials include Cu 2+ The solution contains xanthan gum, sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol, and allantoin, among which Cu 2+ The ratio of the mass of the corresponding copper salt in the solution to the hydrogel is (0.01–0.1) g: 100 mL.

2. The barrier-repairing anti-inflammatory hydrogel according to claim 1, characterized in that, The ratio of xanthan gum to the hydrogel is (0.01-10) g: 100 mL, and the ratio of sodium hyaluronate to the hydrogel is (0.01-1) g: 100 mL.

3. The barrier-repairing anti-inflammatory hydrogel according to claim 1, characterized in that, The Cu 2+ One or more of copper sulfate, copper gluconate, copper citrate, copper carbonate, and copper lactate.

4. The barrier-repairing anti-inflammatory hydrogel according to claim 1, characterized in that, The ratio of butanediol to the hydrogel is (0.1-10) g: 100 mL, and the ratio of glycerol to the hydrogel is (0.01-10) g: 100 mL.

5. The barrier-repairing anti-inflammatory hydrogel according to claim 1, characterized in that, The ratio of ceramide to hydrogel is (0.01-1) g: 100 mL, the ratio of nicotinamide to hydrogel is (0.1-5) g: 100 mL, the ratio of panthenol to hydrogel is (0.1-10) g: 100 mL, and the ratio of allantoin to hydrogel is (0.01-1) g: 100 mL.

6. A method for preparing the barrier-repairing anti-inflammatory hydrogel according to any one of claims 1 to 5, characterized in that, Includes the following steps: S1, containing Cu 2+ Copper salts are dissolved in sterile deionized water to obtain Cu. 2+ The solution was heated, and then xanthan gum was added and mixed well to obtain the initial hydrogel. S2, add sodium hyaluronate, butylene glycol, glycerin, ceramide, nicotinamide, panthenol and allantoin to the initial hydrogel, and then adjust the pH of the resulting system to neutral or weakly acidic to obtain a barrier repair anti-inflammatory hydrogel.

7. The method for preparing the barrier-repairing anti-inflammatory hydrogel according to claim 6, characterized in that, S1 will Cu 2+ Heat the solution to 40-50℃, then add xanthan gum and mix well.

8. The method for preparing the barrier-repairing anti-inflammatory hydrogel according to claim 6, characterized in that, S2 adjusted the pH of the resulting system to 5.8–7.2 to obtain a barrier-repairing anti-inflammatory hydrogel.

9. A barrier-repairing anti-inflammatory hydrogel, characterized in that, It is prepared by any one of claims 6 to 8.

10. The use of the hydrogel of claim 9 in the preparation of topical formulations for treating seborrheic dermatitis and related skin barrier impairment caused by Malassezia furfur.

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