Animal fear behavior observation device and test method

Abstract

The invention provides an animal fear behavior observation device, and particularly relates to the technical field of experimental equipment.The animal fear behavior observation device comprises a box body and a partition plate, the partition plate is arranged in the box body and divides the box body into an upper observation chamber and a bottom electric shock chamber, and the partition plate is arranged in the box body; the bottom of the electric shock chamber is provided with an electric floor grid, the observation chamber can observe the electric shock chamber through the partition plate, the bystander effect can be effectively simulated when observable fear occurs, the behavior reaction of a mouse after an observation partner is subjected to electric shock can be successfully captured, the emotional reaction, behavior and strategy of the mouse can be recorded, analyzed and compared, and the mood response, behavior and strategy of the mouse can be recorded and analyzed. And the influence of social interaction and disgust states on mouse behaviors can be known more deeply.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment technology, and in particular to an animal fear behavior observation device and experimental method. Background Technology

[0002] Fear, as a basic emotional response to potential threats, is crucial for understanding animal survival strategies, social behavior, and evolutionary mechanisms. In the field of biomedical research, especially in neuroscience, behavioral science, and psychology, the study of animal behavioral responses and mechanisms under negative emotions is of great significance. These studies not only help us understand how animals adapt to complex and ever-changing environments, but also provide valuable references for the study of human emotional disorders, psychological problems, and social behaviors.

[0003] In neuroscience and behavioral research, mice are commonly used as experimental animal models. Understanding the impact of the contagion of negative emotions on the behavioral resilience and ability to cope with adversity in mice is crucial. By simulating the emotional stress and challenges faced by humans in mice, researchers can gain a deeper understanding of the pathophysiological processes of human emotional disorders.

[0004] Most existing devices can only simulate and observe the mouse's response to stimuli, but lack the ability to observe the mouse's response after watching other mice receive an electric shock. In view of this, the present invention provides an experimental device that can effectively simulate and observe the emotional contagion, behavioral resilience and ability to cope with adversity of mice after watching their companions receive an electric shock. Summary of the Invention

[0005] To address the aforementioned problems, this invention proposes an animal fear behavior observation device and testing method.

[0006] This invention is achieved through the following technical solution:

[0007] This invention proposes an animal fear behavior observation device comprising a housing and a partition, wherein:

[0008] The partition is installed inside the box and separates the box into an upper observation chamber and a lower electric shock chamber. The bottom of the electric shock chamber is provided with an electric floor grid. The observation chamber can observe the electric shock chamber through the partition.

[0009] Furthermore, the partition is made of a transparent material.

[0010] Furthermore, the partition is provided with 2*16 perforations.

[0011] Furthermore, the diameter of the perforation is 0.5cm-1cm.

[0012] Furthermore, the enclosure is made of a transparent insulating material.

[0013] Furthermore, the dimensions of the box are 38cm*10cm*12.7cm.

[0014] Furthermore, it also includes an electric shock stimulation system, which is electrically connected to the electric floor grid.

[0015] Furthermore, the testing method for the animal fear behavior observation device includes the following steps:

[0016] S1. Place the control mice in the observation room and allow them to adapt for 3 minutes;

[0017] S2. Apply an electric shock to the control mice in the observation room;

[0018] S3. Place the experimental mice in the electric shock chamber and allow them to adapt for 10 minutes.

[0019] S4. Apply electric shocks to the experimental mice, control the intervals and number of shocks, and record the data of the observed mice;

[0020] S5. Return the control mice and experimental mice to their respective cages and record the data for both.

[0021] The beneficial effects of this invention are:

[0022] The animal fear behavior observation device proposed in this invention can effectively simulate the bystander effect when observational fear occurs, and successfully capture the behavioral response of mice after observing their peers being subjected to electric shocks. It can also record and analyze the emotional response, behavior and strategies of control mice, and provide a deeper understanding of the impact of social interaction and aversion on mouse behavior. Attached Figure Description

[0023] Figure 1 This is a structural diagram of the animal fear behavior observation device of the present invention;

[0024] In the diagram: 1. Box body; 2. Partition; 21. Perforation; 3. Electric floor grid.

[0025] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0026] To more clearly and completely illustrate the technical solution of the present invention, the present invention will be further described below with reference to the accompanying drawings.

[0027] Please refer to Figure 1 The present invention proposes an animal fear behavior observation device, comprising a box 1 and a partition 2, wherein:

[0028] The partition 2 is installed inside the box 1, dividing the box 1 into an upper observation chamber and a lower electric shock chamber. The bottom of the electric shock chamber is provided with an electric floor grid 3, and the observation chamber can observe the electric shock chamber through the partition 2.

[0029] In a specific implementation, the four walls of the chamber 1 are made of transparent and insulating material, which is necessary to ensure that the behavioral details of the experimental mice can be clearly observed without disturbing them. The top of the chamber 1 is equipped with a ventilation opening to maintain air circulation. The interior of the chamber 1 is divided by a partition 2. The observation chamber is used to place control mice, the electric shock chamber is used to place experimental mice, and the electric shock grid 3 is used to apply electric shocks to the experimental mice. Control mice can observe the experimental mice through the partition 2. After applying electric shocks to the experimental mice, the bystander effect during observational fear can be effectively simulated, and the behavioral response of mice after observing their peers being shocked can be successfully captured. The emotional response, behavior, and strategies of the control mice can be recorded and analyzed, which can provide a deeper understanding of the impact of social interaction and aversion on mouse behavior.

[0030] Furthermore, partition 2 is made of transparent material.

[0031] Furthermore, the partition 2 is provided with 2*16 perforations 21.

[0032] Furthermore, the diameter of the perforation 21 is 0.5cm-1cm.

[0033] Furthermore, the dimensions of box 1 are 38cm*10cm*12.7cm.

[0034] Furthermore, the enclosure is made of a transparent insulating material.

[0035] In a specific implementation, the box 1 can be made of plastic or other materials depending on the actual situation, as long as insulation and transparency are guaranteed. The size can also be selected according to actual needs. The partition 2 is used to isolate the experimental mice and control mice. The material of the partition 2 can also be selected according to the actual situation. The partition can be replaced with transparent materials of different clarity to study the effect of different visual clarity on the bystander effect. The number and size of the perforations 21 on the partition 2 can also be selected according to the actual situation. The control mice can observe the experimental mice through the partition 2 and the perforations 21 on the partition 2. By separating the observation room and the electric shock room through the partition 2 and placing the experimental mice and control mice, the bystander effect can be effectively simulated.

[0036] Furthermore, it also includes an electric shock stimulation system, which is electrically connected to the electric floor grid 3.

[0037] In a specific implementation, the electric stimulation system is used to precisely control the electric shock parameters of the electric floor grid 3. The electric shock stimulation system can precisely control the current intensity, frequency, and duration of the electric floor grid 3, etc. By using the electric shock stimulation system for precise control, the electric shock can be applied to the experimental mice more effectively, thereby improving the accuracy and reliability of the entire experiment.

[0038] Furthermore, the experimental method for the animal fear behavior observation device includes the following steps:

[0039] S1. Place the control mice in the observation room and allow them to adapt for 3 minutes;

[0040] S2. Apply an electric shock to the control mice in the observation room;

[0041] S3. Place the experimental mice in the electric shock chamber and allow them to adapt for 10 minutes.

[0042] S4. Apply electric shocks to the experimental mice, control the intervals and number of shocks, and record the data of the observed mice;

[0043] S5. Return the control mice and experimental mice to their respective cages and record the data for both.

[0044] In a specific implementation, electric shocks are applied to control mice in the observation room to make them sensitive to aversion. When electric shocks are applied to experimental mice, control mice are more likely to show behavioral changes. When electric shocks are applied to experimental mice, the number of shocks is controlled to be 19, with shock intervals of 30, 60, or 90 seconds, and each shock is 1 mA for 0.5 seconds. After the shocks are completed, the experimental mice and control mice in shock need to be placed in different cages for 1 hour to prevent the experimental mice and control mice from transferring negative emotions. Finally, video recording software is used to record the video and human scoring is used to analyze the behavioral changes of the mice.

[0045] Of course, the present invention may have many other embodiments. Based on this embodiment, other embodiments obtained by those skilled in the art without any creative effort are all within the scope of protection of the present invention.

Claims

1. An animal fear behavior observation device, characterized in that, Includes the enclosure and partitions, of which: The partition is installed inside the box and separates the box into an upper observation chamber and a lower electric shock chamber. The bottom of the electric shock chamber is provided with an electric floor grid. The observation chamber can observe the electric shock chamber through the partition.

2. The animal fear and behavior observation device according to claim 1, characterized in that, The partition is made of transparent material.

3. The animal fear behavior observation device according to claim 2, characterized in that, The partition has 2*16 perforations.

4. The animal fear behavior observation device according to claim 3, characterized in that, The diameter of the perforation is 0.5cm-1cm.

5. The animal fear behavior observation device according to claim 1, characterized in that, The enclosure is made of a transparent insulating material.

6. The animal fear behavior observation device according to claim 5, characterized in that, The dimensions of the box are 38cm*10cm*12.7cm.

7. The animal fear behavior observation device according to claim 1, characterized in that, It also includes an electric shock stimulation system, which is electrically connected to the electric floor grid.

8. A test method for an animal fear behavior observation device according to any one of claims 1-7, characterized in that, It also includes the following steps: S1. Place the control mice in the observation room and allow them to adapt for 3 minutes; S2. Apply an electric shock to the control mice in the observation room; S3. Place the experimental mice in the electric shock chamber and allow them to adapt for 10 minutes. S4. Apply electric shocks to the experimental mice, control the intervals and number of shocks, and record the data of the observed mice; S5. Return the control mice and experimental mice to their respective cages and record the data for both.

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