Bacterial drug sensitivity detection assembly based on ATP luciferase method and use method

Abstract

The invention relates to the technical field of bacterial detection, in particular to a bacterial drug sensitivity detection assembly based on an ATP luciferase method and a using method.The bacterial drug sensitivity detection assembly comprises a supporting assembly, a sampling assembly, an ATP fluorescence detector and a 96-hole drug sensitivity plate, and a sealing block is arranged at the top of the inner wall of the ATP fluorescence detector; the supporting assembly comprises a base, a controller is arranged on one side of the base, and an electric push rod is arranged in the middle of the upper surface of the base. The device effectively realizes detection of bacterial drug sensitivity of a sample, is high in detection precision and good in detection effect, can also mix and stir the sample in the 96-hole drug sensitive plate, and avoids precipitation of bacteria in the 96-hole drug sensitive plate due to long-time standing; the sampling quantity of samples can be accurately detected, and the accuracy and the stability of subsequent detection are ensured; when the sample in the sampling tube is mixed with the reaction liquid, the flowing efficiency of the sample can be ensured, and the problems of high liquid feeding difficulty, poor mixing effect and the like caused by negative pressure are avoided.

Description

Technical Field

[0001] This invention relates to the field of bacterial detection technology, and in particular to a bacterial drug susceptibility detection component and its usage method based on the ATP luciferase method. Background Technology

[0002] ATP, short for adenosine triphosphate, is a substance widely found in the cells of bacteria and other microorganisms. ATP is the energy substance for microbial metabolism. The ATP bioluminescence method utilizes the reaction of several components in the ATP reagent, such as luciferin and luciferase, with the sample to generate photons. A specially developed ATP fluorescence detector is then used to capture and detect the luminescence value. Since the number of bacteria and other microorganisms in the sample is positively correlated with the ATP content and the ATP value, the content of bacteria and other microorganisms in the sample can be obtained by detecting the luminescence value. In the ATP detection process, the sample is cultured inside a 96-well susceptibility plate. Swabs are usually used, which are generally small clumps of absorbent material wrapped around one end of a stick to sample microorganisms and exfoliated cells, facilitating the study of ATP data. This allows for the measurement of overall information from a small portion of the ATP sample.

[0003] The method for detecting bacterial drug susceptibility using the TTC reduction reaction, published in CN111088318A, involves: preparing an antibiotic solution; preparing a 96-well plate with a gradient of antibiotic concentrations; preparing a bacterial suspension; incubating at a constant temperature; and interpreting the results. The medium turns red after a color reaction, indicating that the tested bacteria can tolerate that concentration of antibiotic. This allows for sensitive and accurate determination of the drug susceptibility test results, yielding the minimum inhibitory concentration (MIC). However, this method has low precision and poor detection effect.

[0004] Compared to using the TTC reduction reaction to detect bacterial drug susceptibility, the ATP luciferase method offers higher accuracy. However, the ATP luciferase method requires bacterial sample collection, which is traditionally done using ATP swabs. This requires breaking the swab during sampling to ensure the reaction solution mixes with the sample, a method that is inconvenient and makes it difficult to guarantee complete release of the reaction solution.

[0005] When sampling tubes are used to sample the inside of a 96-well antimicrobial susceptibility plate, the liquid level inside the plate varies due to continuous sampling, making continuous and accurate sampling impossible. This reduces the accuracy of subsequent sample lysis and reaction. Furthermore, prolonged standing of the samples inside the 96-well plate can cause precipitation, resulting in different sample concentrations at different heights within the plate, further reducing the accuracy of sample detection.

[0006] When the sampling tube is flipped over and the sample inside is mixed with the reaction solution, the negative pressure inside the sampling tube will cause the sample to flow down slowly, thereby reducing the mixing accuracy with the reaction solution. In addition, some sample will adhere to the internal structure of the sampling tube, thereby reducing the mixing reaction effect between the sample and the reaction solution, and ultimately reducing the accuracy of bacterial drug sensitivity detection of the sample.

[0007] Therefore, it is necessary to invent a bacterial drug susceptibility detection component based on the ATP luciferase method and its usage method to solve the above problems. Summary of the Invention

[0008] The purpose of this invention is to provide a bacterial drug susceptibility detection component and its usage method based on the ATP luciferase method, so as to solve the problems mentioned in the background art.

[0009] To achieve the above objectives, the present invention provides the following technical solution: a bacterial drug susceptibility detection component based on the ATP luciferase method, comprising a support component, a sampling component, an ATP fluorescence detector, and a 96-well drug susceptibility plate, wherein a sealing block is provided on the top of the inner wall of the ATP fluorescence detector;

[0010] The support assembly includes a base, a controller is provided on one side of the base, and an electric push rod is provided in the middle of the upper surface of the base;

[0011] The sampling assembly includes a sampling tube with an open slot extending through its outer bottom end. A fixing frame is located in the middle of the inner side of the sampling tube. A movable plug is located below the fixing frame. A transmission rod is located at the bottom end of the movable plug. A pressure block is located at the bottom end of the transmission rod. A distance sensor is located at the end of the pressure block. A top block is located on the outer side of the pressure block. A fixing plate is located at the bottom end of the top block, and the fixing plate is fixedly mounted on the top of the ATP fluorescence detector.

[0012] The sampling tube has multiple sets of sleeves on one side, and a sliding block is slidably connected inside the sleeve. The sampling tube has a middle channel inside, and one side of the multiple sets of sleeves is connected to the middle channel through an exhaust hole. A one-way exhaust valve is provided inside the exhaust hole. An impact hole is provided inside the movable plug. A floating plate is movably connected inside the sampling tube, and multiple sets of counterweight rods are provided at the bottom of the floating plate.

[0013] Before actually sampling the samples inside the 96-well antimicrobial susceptibility plate, the device mixes and stirs them to improve the mixing effect. As the sampling tube is flipped, the samples inside the sampling tube fall into the reaction liquid and undergo a mixing reaction. At the same time, when the sampling tube moves, the gas inside the sleeve can also exert a force on the floating plate, thereby realizing the up and down movement of the floating plate and the movable plug inside the sampling tube, improving the flow integrity of the liquid inside the sampling tube.

[0014] Preferably, the sampling component is provided in two sets, and the two sets of sampling components are respectively disposed above the ATP fluorescence detector and the 96-well drug sensitivity plate. The sampling component, the ATP fluorescence detector and the 96-well drug sensitivity plate are all disposed above the support component.

[0015] Preferably, the sleeve has a connecting spring on its side wall, the other side of the connecting spring is fixedly connected to the side wall of the sliding block, the floating plate has a misalignment groove on one side, the misalignment groove matches the transmission rod, the intermediate channel matches the impact hole, the top output end of the impact hole matches the bottom of the floating plate, the controller electrically controls each electrical component, and the distance sensor is used to detect the distance value between itself and the floating plate.

[0016] Preferably, the upper surface of the base is provided with fixed rods at both ends, and the top of the fixed rods is provided with support seats. The ATP fluorescence detector and the 96-well drug sensitivity plate are respectively set above the two support seats. The top of the electric push rod is movably provided with a movable frame through a bearing. Both ends of the movable frame are provided with connecting rods. The bottom of the connecting rod is provided with a "door" shaped bracket. Movable seats are provided through the bottom of the inner walls on both sides of the bracket. A bushing is provided on the inner side of the movable seat. A buffer spring is provided between the bushing and the movable seat.

[0017] Preferably, one end of the movable seat is provided with a transmission bevel gear, the outer side of the transmission bevel gear is provided with a drive bevel gear, the top end of the drive bevel gear is provided with a transmission shaft, and the transmission shaft is located on the outer side of the bracket. The top end of the transmission shaft is provided with a transmission gear, the outer side of the transmission gear is provided with an arc-shaped rack, there are two arc-shaped racks, the top end of the arc-shaped rack is provided with a top plate, and the top plate is fixedly located on the top end of the electric push rod.

[0018] Preferably, the top of the electric push rod is provided with a positioning post, the outer side of the positioning post is provided with a slot, the inside of the slot is provided with an elastic limiting block, a return spring is provided between the elastic limiting block and the slot, and one end of the elastic limiting block is provided with a hemispherical structure. The outer side of the positioning post is provided with a positioning ring, and the positioning ring is fixedly set above the movable frame. The inner side of the positioning ring is provided with two limiting grooves corresponding to the bracket, and the limiting grooves correspond to the elastic limiting block.

[0019] Preferably, the sampling tube is positioned above the base, and a hexagonal shaft is provided in the middle of both sides of the sampling tube, with the bushing adapted to the hexagonal shaft. The lower surface of the fixing frame is provided with multiple liquid guide tubes, and multiple liquid guide holes are provided around the bottom outer side of the liquid guide tubes. Multiple through grooves are provided through the middle of the movable plug, and the liquid guide tubes are located inside the through grooves, with the bottom end of the liquid guide tubes flush with the opening of the through grooves.

[0020] Preferably, the top center of the movable plug has a groove, the inside of the groove has a compression spring, and the top of the compression spring is located on the lower surface of the fixed frame. The outer side of the pressure block is set with an arc-shaped structure, and the pressure block is located inside the opening groove.

[0021] Preferably, the bottom of the sampling tube is provided with lysis fluid, and the liquid level of the lysis fluid is located below the opening groove. The top of the sampling tube is provided with reaction fluid, and the reaction fluid is located above the movable plug.

[0022] A method for using a bacterial drug susceptibility detection kit based on the ATP luciferase method, comprising the following steps:

[0023] Step 1: Sample pretreatment. Remove the sampling tube from the refrigeration equipment and let it stand until it returns to room temperature. Then install it on the support assembly. During installation, ensure that the opening groove of the sampling tube is at the bottom and that the lysis solution in the sampling tube does not leak. At the same time, place the 96-well antimicrobial susceptibility plate containing the bacteria to be tested on top of the support assembly.

[0024] Step 2: Sampling. The controller moves the electric push rod downwards, inserting the bottom of the sampling tube into the 96-well antimicrobial susceptibility plate. The top block and the sliding block press against each other and drive the movable frame to rotate around the electric push rod. The sampling tube then rotates back and forth inside the 96-well antimicrobial susceptibility plate to stir. After stirring is complete, the sampling tube continues to move downwards, and the sample enters the sampling tube through the opening groove and mixes with the lysis solution in the sampling tube to produce a lysis reaction.

[0025] Step 3: Catalytic reaction. By adjusting the position of the sampling tube, the sampling tube moves above the ATP fluorescence detector. The sampling tube flips over, and the position of the movable plug inside the sampling tube changes. The sampling tube continues to move upward, causing the sealing block on the inner wall of the ATP fluorescence detector to press against the sliding block. The sliding block moves along the sleeve, causing the internal gas to exert a force on the bottom of the floating plate along the exhaust port, the middle channel, and the impact hole. The floating plate moves up and down inside the sampling tube, driving the movable plug to move up and down. The lysed sample is completely mixed with the reaction solution. The luciferase in the reaction solution catalyzes the reaction of luciferin with oxygen, causing the sample to emit fluorescence.

[0026] Step 4: Detection. The ATP fluorescence detector continues to move upward, allowing the sampling tube to be inserted into the detection port of the ATP fluorescence detector. The fluorescence intensity of the sample is detected by the ATP fluorescence detector, thereby realizing the drug sensitivity detection of the sample. During this process, another set of the sampling components and the 96-well drug sensitivity plate are replaced and sampled to realize the simultaneous detection of multiple sets of samples.

[0027] The technical effects and advantages of this invention are as follows:

[0028] 1. By setting up a support component, this invention, compared to using a breakable ATP swab, allows the sample to enter the reaction solution storage area by flipping the sampling tube, thus ensuring that all the reaction solution can participate in the reaction and thereby guaranteeing detection accuracy.

[0029] 2. By setting up a sampling component, during the process of inserting the sampling tube into the detection port of the ATP fluorescence detector, the pressure block is squeezed by the top block, which drives the movable plug to move, thereby opening the channel inside the sampling tube and allowing the sample to automatically enter the reaction solution, thus facilitating the detection work.

[0030] 3. By setting up a support component, the present invention allows the two support seats to lift the ATP fluorescence detector and the 96-well drug sensitivity plate respectively, thereby facilitating the sampling and detection work. The support can support and position the sampling tube. At the same time, the support can rotate around the electric push rod to flip the sampling tube, thereby ensuring that the lysis solution and sample in the sampling tube can react fully, thus ensuring the accuracy of the reaction.

[0031] 4. By incorporating a floating plate and impact orifices, this invention effectively achieves bacterial drug sensitivity testing of samples with high accuracy and good results. It also mixes and stirs samples within the 96-well drug sensitivity plate, preventing bacterial sedimentation due to prolonged static conditions. Furthermore, it accurately detects the sample quantity, ensuring the accuracy and stability of subsequent tests. When mixing samples with the reaction solution inside the sampling tube, it ensures efficient sample flow, avoiding problems such as difficulty in dispensing and poor mixing due to negative pressure. The up-and-down vibration of the movable plug and floating plate within the sampling tube improves the thoroughness of sample dispensing and the cleanliness of the internal structure, further guaranteeing the mixing effect between the sample and the reaction solution and the accuracy of subsequent tests. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0033] Figure 2 This is a schematic diagram of the support component structure of the present invention.

[0034] Figure 3This is a side view of the support component structure of the present invention.

[0035] Figure 4 This is a cross-sectional view of the support component structure of the present invention.

[0036] Figure 5 This is a schematic diagram of the movable frame structure of the present invention.

[0037] Figure 6 This is a schematic diagram of the top block structure of the present invention.

[0038] Figure 7 This is a schematic diagram showing the positional relationship between the top block and the pressure block in this invention.

[0039] Figure 8 This is a schematic diagram of the sampling tube structure of the present invention.

[0040] Figure 9 This is a schematic diagram of the movable plug structure of the present invention.

[0041] Figure 10 This is a schematic diagram of the internal structure of the sampling tube of the present invention.

[0042] Figure 11 This is a schematic diagram of the support structure of the present invention.

[0043] Figure 12 This is a schematic cross-sectional view of the sampling tube structure of the present invention.

[0044] Figure 13 for Figure 12 Enlarged diagram of point A in the middle.

[0045] In the diagram: 1. Support assembly; 2. Sampling assembly; 3. ATP fluorescence detector; 4. 96-well drug sensitivity plate; 101. Base; 102. Fixing rod; 103. Support seat; 104. Electric push rod; 105. Movable frame; 106. Connecting rod; 107. Bracket; 108. Movable seat; 109. Bushing; 110. Transmission bevel gear; 111. Drive bevel gear; 112. Transmission shaft; 113. Transmission gear; 114. Arc rack; 115. Top plate; 116. Positioning post; 117. Slot; 118. Elastic limit block; 11 9. Positioning ring; 120. Limiting groove; 201. Sampling tube; 202. Hexagonal shaft; 203. Opening groove; 204. Fixing frame; 205. Liquid guide tube; 206. Liquid guide hole; 207. Movable plug; 208. Through groove; 209. Groove; 210. Transmission rod; 211. Pressure block; 212. Top block; 213. Fixing plate; 214. Floating plate; 215. Misalignment groove; 216. Counterweight rod; 217. Sleeve; 218. Sliding block; 219. Connecting spring; 220. Intermediate channel; 221. Vent hole; 222. Impact hole. Detailed Implementation

[0046] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0047] First Embodiment

[0048] This invention provides, for example Figures 1 to 12 The bacterial drug susceptibility detection component based on the ATP luciferase method shown includes a support component 1, a sampling component 2, an ATP fluorescence detector 3, and a 96-well drug susceptibility plate 4. Two sets of sampling components 2 are respectively positioned above the ATP fluorescence detector 3 and the 96-well drug susceptibility plate 4. The sampling component 2, ATP fluorescence detector 3, and 96-well drug susceptibility plate 4 are all positioned above the support component 1. The ATP fluorescence detector 3 detects the sample after the chemical reaction, the 96-well drug susceptibility plate 4 incubates the sample, and the support component 1 supports the ATP fluorescence detector 3. The 96-well drug sensitivity plate 4 is supported by the sampling component 2, which extracts samples from inside the 96-well drug sensitivity plate 4 and transfers them to the ATP fluorescence detector 3 for detection. The 96-well drug sensitivity plate 4 has multiple sampling wells arranged in a uniform array inside. A driving component is provided between the 96-well drug sensitivity plate 4 and the support component 1. The driving component can be two sets of electric telescopic rods. The electric telescopic rods push the 96-well drug sensitivity plate 4 so that the sampling component 2 can move stably to the top of the sampling wells at the top of the 96-well drug sensitivity plate 4, and ensure that the sampling component 2 can extract all the samples from the sampling wells at the top of the 96-well drug sensitivity plate 4.

[0049] The support assembly 1 includes a base 101. A controller is provided on one side of the base 101, which electrically controls various electrical components. Fixed rods 102 are provided at both ends of the upper surface of the base 101. A support seat 103 is provided at the top of each fixed rod 102. The ATP fluorescence detector 3 and the 96-well drug sensitivity plate 4 are respectively positioned above the two support seats 103. An electric push rod 104 is provided in the middle of the upper surface of the base 101. A movable frame 105 is movably mounted at the top of the electric push rod 104 via a bearing. Connecting rods 106 are provided at both ends of the movable frame 105. The bottom end is provided with a "door" shaped support 107. The bottom ends of the inner walls on both sides of the support 107 are provided with movable seats 108. The inner side of the movable seat 108 is provided with a bushing 109. A buffer spring is provided between the bushing 109 and the movable seat 108. The bushing 109 is adapted to the hexagonal shaft 202. A self-locking mechanism is provided between the movable seat 108 and the bushing 109. The self-locking mechanism allows the movable seat 108 to drive the bushing 109 to rotate, while the bushing 109 cannot drive the movable seat 108 to rotate, thus ensuring that the position of the sampling tube 201 can remain fixed after it is flipped.

[0050] One end of a movable seat 108 is provided with a transmission bevel gear 110, and the outer side of the transmission bevel gear 110 is provided with a drive bevel gear 111. The top end of the drive bevel gear 111 is provided with a transmission shaft 112, and the transmission shaft 112 is located on the outer side of the bracket 107. The top end of the transmission shaft 112 is provided with a transmission gear 113, and the outer side of the transmission gear 113 is provided with an arc-shaped rack 114. There are two arc-shaped racks 114. The top end of the arc-shaped rack 114 is provided with a top plate 115, and the top plate 115 is fixedly located on the top end of the electric push rod 104. When the transmission gear 113 passes through the arc-shaped rack 114, its rotation number can flip the sampling tube 201, and can ensure that the sampling tube 201 flips in the opposite direction to the opening groove 203, thereby preventing the sample from leaking through the opening groove 203.

[0051] Furthermore, the top of the electric push rod 104 is provided with a positioning post 116, and a slot 117 is provided on the outside of the positioning post 116. An elastic limiting block 118 is provided inside the slot 117. A spring is provided between the elastic limiting block 118 and the slot 117, and one end of the elastic limiting block 118 is set as a hemispherical structure. A positioning ring 119 is provided on the outside of the positioning post 116, and the positioning ring 119 is fixedly set above the movable frame 105. Two limiting grooves 120 corresponding to the bracket 107 are opened on the inside of the positioning ring 119, and the limiting grooves 120 correspond to the elastic limiting block 118. When the elastic limiting block 118 is inserted into the limiting groove 120, the sampling component 2 is located above the ATP fluorescence detector 3. The setting of the elastic limiting block 118 and the limiting groove 120 facilitates the rapid positioning of the sampling component 2.

[0052] The sampling assembly 2 includes a sampling tube 201, which is positioned above the base 101. A hexagonal shaft 202 is located at the center of both sides of the sampling tube 201. An opening groove 203 is provided through the bottom outer end of the sampling tube 201. A fixing frame 204 is located at the center of the inner side of the sampling tube 201. Multiple liquid guide tubes 205 are provided on the lower surface of the fixing frame 204. Multiple liquid guide holes 206 are arranged around the bottom outer end of the liquid guide tubes 205. A movable plug 207 is located below the fixing frame 204. Multiple through grooves 208 are provided through the center of the movable plug 207. The liquid guide tubes 205 are located inside the through grooves 208, and the bottom end of the liquid guide tubes 205 is flush with the opening of the through grooves 208. Therefore, when the movable plug 207 and the liquid guide tubes 205 are misaligned, the sample inside the sampling tube 201 can enter the other end of the sampling tube 201 along the liquid guide holes 206.

[0053] The movable plug 207 has a groove 209 at the top center, and a compression spring is provided inside the groove 209. The top of the compression spring is located on the lower surface of the fixed frame 204. The bottom end of the movable plug 207 is provided with a transmission rod 210, and the bottom end of the transmission rod 210 is provided with a pressure block 211. The outer side of the pressure block 211 is set with an arc-shaped structure, and the pressure block 211 is located inside the opening groove 203. The outer side of the pressure block 211 is provided with a top block 212, and the bottom end of the top block 212 is provided with a fixing plate 213. The fixing plate 213 is fixedly set at the top of the ATP fluorescence detector 3. The setting of the fixing plate 213 allows the top block 212 to twist when it is squeezed by the outer wall of the sampling tube 201, thereby ensuring that the top block 212 can move into the opening groove 203.

[0054] The sampling tube 201 has a lysis buffer at its bottom, with the liquid level below the opening groove 203. The sampling tube 201 has a reaction solution at its top, positioned above the movable stopper 207. After extraction, the sample is first mixed with the lysis buffer and then with the reaction solution. The luciferase in the reaction solution catalyzes the reaction of luciferin with oxygen, emitting fluorescence using the energy released from ATP. The fluorescence intensity is proportional to the amount of ATP. The instrument can read the fluorescence intensity and output the RLU value, reflecting the amount of ATP. This allows the range of bacterial cell content in the sample to be calculated, thus enabling the detection of bacterial drug susceptibility.

[0055] When using, remove the sampling tube 201 from the refrigeration equipment in the sampling assembly 2 and let it stand until it returns to room temperature. Then install it on the support assembly 1. During the installation process, ensure that the opening groove 203 of the sampling tube 201 is at the bottom and that the lysis solution in the sampling tube 201 does not leak. At the same time, place the 96-well antimicrobial susceptibility plate 4 containing the bacteria to be tested on top of the support assembly 1.

[0056] By adjusting the height of the sampling tube 201, the bottom end of the sampling tube 201 is inserted into the 96-well antimicrobial susceptibility plate 4. At this time, the sample can enter the sampling tube 201 through the opening groove 203 and mix with the lysis solution in the sampling tube 201 to produce a lysis reaction. The lysis solution will cause the bacteria in the sample to lyse and release the ATP in the bacteria in the sample.

[0057] The bottom end of the sampling tube 201 is inserted into the 96-well antimicrobial susceptibility plate 4. When the sample liquid level in the 96-well antimicrobial susceptibility plate 4 is higher than the opening groove 203, the sample above the opening groove 203 enters the sampling tube 201 and mixes with the lysis solution in the sampling tube 201 to react. At this time, the sample extraction can be completed.

[0058] By adjusting the position of the sampling tube 201, the sampling tube 201 is moved above the ATP fluorescence detector 3. During this process, the sampling tube 201 is flipped. At this time, the height of the ATP fluorescence detector 3 is adjusted, and the position of the movable plug 207 inside the sampling tube 201 changes. The lysed sample is mixed with the reaction solution. The luciferase in the reaction solution catalyzes the reaction of luciferin with oxygen, causing the sample to emit fluorescence.

[0059] When the position of the sampling tube 201 is adjusted, the movable frame 105 drives the support 107 to rotate around the electric push rod 104. The support 107 drives the sampling tube 201 and the drive shaft 112 to rotate. During the rotation, the transmission gear 113 at the top of the drive shaft 112 meshes with the arc rack 114. At this time, the transmission gear 113 drives the drive shaft 112 to rotate. The drive shaft 112 drives the movable seat 108 to rotate through the drive bevel gear 111 and the transmission bevel gear 110. The movable seat 108 drives the sampling tube 201 to rotate through the bushing 109 and the hexagonal shaft 202, causing the sampling tube 201 to flip. At this time, the sampling tube 201 flips in the opposite direction of the opening groove 203. The lysis solution and the sample are fully mixed during the flipping process.

[0060] When the sampling tube 201 moves above the ATP fluorescence detector 3, the ATP fluorescence detector 3 drives the top block 212 to move upward through the fixing plate 213. The sampling tube 201 rotates due to the pressure of the top block 212. When the top block 212 moves into the opening groove 203, the top block 212 squeezes the pressure block 211, causing the pressure block 211 to drive the transmission rod 210 to move. The transmission rod 210 can drive the movable plug 207 to move downward. At this time, the position between the movable plug 207 and the liquid guide tube 205 changes. The sample above the movable plug 207 can enter below the movable plug 207 through the liquid guide hole 206 and the liquid guide tube 205, and mix with the reaction liquid below the movable plug 207 to produce a fluorescence reaction.

[0061] The ATP fluorescence detector 3 continues to move upward, allowing the sampling tube 201 to be inserted into the detection port of the ATP fluorescence detector 3. At this time, the fluorescence intensity of the sample can be detected by the ATP fluorescence detector 3, thereby enabling the drug sensitivity detection of the sample. During this process, another set of sampling components 2 and the 96-well drug sensitivity plate 4 can be replaced and sampled to achieve simultaneous detection of multiple sets of samples.

[0062] Second Embodiment

[0063] like Figure 13As shown, when sampling is performed on the 96-well antimicrobial susceptibility plate 4 using sampling tube 201, the liquid level inside the 96-well antimicrobial susceptibility plate 4 varies due to continuous sampling and testing. Therefore, it is impossible to continuously and accurately detect the sample volume inside sampling tube 201. Furthermore, since sampling tube 201 can only sample the top of the 96-well antimicrobial susceptibility plate 4, and the concentration of the sample inside the 96-well antimicrobial susceptibility plate 4 varies due to long-term deposition, the sampling tube 201 will have errors in the sampling concentration of the sample inside the 96-well antimicrobial susceptibility plate 4, thus reducing the detection accuracy. When the sample inside sampling tube 201 flows into the liquid guide hole 206 and mixes with the reaction solution, the sample's own gravity alone will cause the reaction solution to flow slowly, resulting in low flow accuracy. Additionally, some sample will adhere to the surface of sampling tube 201 and movable plug 207, thus reducing the mixing effect between the sample and the reaction solution.

[0064] To address the aforementioned issues, the bacterial drug susceptibility detection component based on the ATP luciferase method further includes a distance sensor at the end of the pressure block 211, which is used to detect the distance between the block and the floating plate 214.

[0065] The sampling tube 201 has multiple sets of sleeves 217 on one side. Gas is contained inside the sleeves 217. A sliding block 218 is slidably connected inside the sleeves 217. A connecting spring 219 is provided on the side wall of the sleeves 217. The other side of the connecting spring 219 is fixedly connected to the side wall of the sliding block 218. The connecting spring 219 effectively improves the elastic reset performance of the sliding block 218.

[0066] The sampling tube 201 has an internal intermediate channel 220. One side of the multiple sets of sleeves 217 is connected to the intermediate channel 220 through an exhaust port 221. The exhaust port 221 has a one-way vent valve, and the direction of the one-way vent valve is to enter the intermediate channel 220 along the sleeve 217. When the sliding block 218 is compressed, the sliding block 218 compresses the connecting spring 219 inside the sleeve 217 and moves towards the end of the sampling tube 201. The gas inside the sleeve 217 enters the intermediate channel 220 unidirectionally along the exhaust port 221. The movable plug 207 has an impact hole 222 inside, and the intermediate channel 220 matches the impact hole 222. Since the movable plug 207 is not compressed and is in its initial position in the initial state, the movable plug 207 blocks the intermediate channel 220. At this time, the gas inside the sleeve 217 cannot enter the intermediate channel 220 along the exhaust hole 221. When the movable plug 207 is compressed and moves downward to the maximum distance, the intermediate channel 220 is connected to the impact hole 222. Then the gas inside the intermediate channel 220 can be discharged along the impact hole 222. The top output end of the impact hole 222 matches the bottom of the floating plate 214. Then the gas discharged along the impact hole 222 exerts a force on the floating plate 214 and drives the floating plate 214 to move upward.

[0067] A floating plate 214 is movably connected inside the sampling tube 201. Multiple sets of counterweight rods 216 are provided at the bottom of the floating plate 214. Therefore, when there is a sample inside the sampling tube 201, the floating plate 214 is positioned on top of the sample by the buoyancy of the sample. A misalignment groove 215 is provided on one side of the floating plate 214. The misalignment groove 215 matches the transmission rod 210. When the sampling tube 201 is rotated 180 degrees, the floating plate 214 is rotated accordingly, and the misalignment groove 215 matches the transmission rod 210. A sealing block is provided at the top of the inner wall of the ATP fluorescence detector 3. When the electric push rod 104 drives the sampling tube 201 to continue to move downward inside the ATP fluorescence detector 3, the sliding block 218 and the sealing block are interlocked and squeezed, thereby realizing the up-and-down vibration of the movable plug 207 and the floating plate 214 inside the sampling tube 201, which effectively improves the air pressure discharge of the residual sample inside the sampling tube 201 and the cleaning effect of the inner wall.

[0068] The inner wall end of the sleeve 217 is provided with a stop block, which matches the sliding block 218. Initially, the sliding block 218 is located at the end of the stop block. When the intermediate channel 220 is not connected to the impact hole 222, the outer end of the sliding block 218 cannot slide along the inner wall of the sleeve 217 when it is squeezed.

[0069] In use, as shown in the first embodiment, a sample is added inside the 96-well drug sensitivity plate 4, and the sampling tubes 201 are placed inside both sides of the support assembly 1. The two sets of hexagonal shafts 202 are fixed and limited by the bushings 109, so that the two sets of sampling tubes 201 are respectively located above the ATP fluorescence detector 3 and the 96-well drug sensitivity plate 4. Then, the controller controls the electric push rod 104 to start and drive the movable frame 105 downwards. The movable frame 105 drives the sampling tubes 201 on both sides downwards. When the sample inside the 96-well drug sensitivity plate 4 reaches the opening groove 203, the sample inside the 96-well drug sensitivity plate 4 continuously enters the sampling tube 201 along the opening groove 203. The sample comes into contact with the lysis buffer on one side of the sampling tube 201, causing lysis and effectively improving the accuracy and effectiveness of subsequent sample detection. As the sample volume on one side of the sampling tube 201 increases, the buoyancy of the sample and lysis buffer on one side of the sampling tube 201 drives the floating plate 214 to move upward, and the distance value detected by the distance detector continuously decreases. This indicates that the sample inside the 96-well drug sensitivity plate 4 is continuously entering the sampling tube 201. When the distance value detected by the distance sensor reaches the preset distance value, it indicates that the amount of sample inside the 96-well drug sensitivity plate 4 entering the sampling tube 201 meets the requirements, thus effectively ensuring the accuracy and requirements of subsequent sample detection.

[0070] Then, the elastic limiting block 118 is manually moved and disengaged from the limiting groove 120. The movable frame 105 is rotated so that the sampling tube 201 above the 96-well drug sensitivity plate 4 moves to the ATP fluorescence detector 3. At the same time, during the rotation, the transmission shaft 112 is driven to rotate by the meshing of the arc rack 114 and the transmission gear 113. The rotation of the transmission shaft 112 drives the drive bevel gear 111 to rotate. The rotation of the drive bevel gear 111 drives the movable seat 108 to rotate through the transmission bevel gear 110. The movable seat 108 drives the sampling tube 201 to rotate through the bushing 109, and the sampling tube 201 rotates 180 degrees. At this time, the sample inside the sampling tube 201 flows along the inner wall to the upper part of the movable plug 207. The floating plate 214 rotates synchronously and reaches the upper part of the movable plug 207. It is also misaligned and engaged with the transmission rod 210 by the misalignment groove 215, which effectively improves the mixing of the subsequent sample and reaction solution and the vibration cleaning of the inner wall of the sampling tube 201.

[0071] When the sampling tube 201 moves above the ATP fluorescence detector 3 and is blocked by the fixing plate 213, the elastic limiting block 118 is released and inserted into the limiting groove 120 for fixation. At the same time, a new sampling tube 201 is fixed inside the support 107 above the 96-well drug sensitivity plate 4 on the other side, thereby realizing continuous mixed detection, obtaining multiple sets of detection data, and effectively improving detection accuracy and precision.

[0072] When the sampling tube 201 located above the 96-well drug sensitivity plate 4 reaches the interior of the 96-well drug sensitivity plate 4 and partially inserts into the sample, the controller simultaneously activates the electric push rod 104, causing the sampling tubes 201 on both sides to move downwards. This downward movement of the sampling tubes 201 causes multiple sets of sliding blocks 218 to move downwards. When the sliding blocks 218 and the top block 212 press against each other, because the movable plug 207 is at its initial height, the intermediate channel 220 is not connected to the impact hole 222, and the multiple sets of sliding blocks 218 are at their initial distance and interlocked with the inner wall of the sleeve 217, the sliding blocks 218 cannot move along the sleeve 217 towards the end of the sampling tube 201. Therefore, the pressing force applied by the top block 212 to the sliding blocks 218 causes the movable frame 105 to press against the elastic limiting block 11. When the electric push rod 104 rotates, the movable frame 105 drives the sampling tube 201 on the other side to rotate laterally inside the 96-well drug sensitivity plate 4. When the sampling tube 201 above the ATP fluorescence detector 3 passes the sliding block 218, under the elastic force of the elastic limit block 118, it drives the movable frame 105 to rotate in the opposite direction around the electric push rod 104 to return to its original position. When the electric push rod 104 drives the movable frame 105 to move downward continuously, the movable frame 105 will continuously reciprocate and twist. With the help of this twisting, the sampling tube 201 will be stirred inside the 96-well drug sensitivity plate 4, thereby effectively improving the mixing effect inside the 96-well drug sensitivity plate 4 and avoiding precipitation caused by long-term standing of the mixture inside the 96-well drug sensitivity plate 4, which would reduce the subsequent detection accuracy and detection effect.

[0073] When the electric push rod 104 drives the movable frame 105 to move continuously and reach a certain value, the top block 212 disengages from the side wall of the sampling tube 201 and moves along the opening groove 203 into the sampling tube 201. At this time, the wedge-shaped squeezing contact between the top block 212 and the pressure block 211 causes the pressure block 211 to move downward. When the pressure block 211 moves downward, it drives the movable plug 207 to move downward through the transmission rod 210. The movable plug 207 moves downward along the liquid guide tube 205 and is misaligned with the liquid guide hole 206. The sample above the movable plug 207 enters the inner cavity of the sampling tube 201 along the liquid guide hole 206 and the liquid guide tube 205, and is fully mixed with the reaction liquid inside the inner cavity, thereby facilitating the subsequent detection accuracy of the sample's drug sensitivity using the ATP fluorescence detector 3.

[0074] As the sample flows downward, the buoyancy of the floating plate 214 gradually decreases, causing the floating plate 214 to move the counterweight rod 216 at the bottom downward. When the bottom of the counterweight rod 216 comes into contact with the top of the movable plug 207, the floating plate 214 stops moving downward. Therefore, by moving the floating plate 214 downward and applying pressure to the sample at the bottom, the effect of mixing the sample as it enters the sampling tube 201 through the liquid guide hole 206 and the liquid guide tube 205 is effectively improved, and some samples are prevented from entering the inner cavity of the sampling tube 201 through the liquid guide hole 206 due to air pressure.

[0075] At this time, the sampling tube 201 located above the 96-well susceptibility plate 4 needs to be sampled. The controller then controls the electric push rod 104 to move the movable frame 105 downwards. The movable frame 105 moves the sampling tubes 201 on both sides downwards. When the sampling tube 201 located above the ATP fluorescence detector 3 moves downwards, the pressure block 211 disengages from the top block 212 and moves downwards. At this time, because the top of the movable plug 207 is still at its lowest point due to the pressure from the counterweight rod 216 and the floating plate 214, and the middle channel 220 is connected to the impact hole 222, the sampling tube 201... When the sliding block 218 moves downward and is inserted into the ATP fluorescence detector 3, it presses against the sealing block at the top of the inner wall of the ATP fluorescence detector 3. Under this pressing force, the sliding block 218 moves along the sleeve 217 towards the side wall of the sampling tube 201. When the sliding block 218 moves, it unidirectionally compresses the gas inside the sleeve 217 into the middle channel 220 through the exhaust hole 221, and then enters the impact hole 222 through the middle channel 220. The gas is discharged upward through the impact hole 222 and exerts an upward force on the floating plate 214. The floating plate 214 is subjected to this force. As the movable counterweight 216 moves upward, the pressure on the movable plug 207 decreases. Under the force of the compression spring, the movable plug 207 moves upward a certain distance. Once the sealing block separates from the sliding block 218, the gas inside the sleeve 217 no longer escapes. Under the gravity of the floating plate 214 and the counterweight 216, the movable plug 207 is forced to move downward again and return to its maximum distance. The intermediate channel 220 reconnects with the impact hole 222. As the sampling tube 201 continues to move downward, the sealing block and multiple sets of sliding blocks 218 press against each other and come into contact. As the movable plug 207 moves inside the sampling tube 201, it continuously moves up and down. This movement not only vibrates and feeds the sample above the inner wall of the sampling tube 201 and the movable plug 207, improving the integrity and thoroughness of the sample entering the reaction solution along the liquid guide hole 206 and the liquid guide tube 205, but also, through the sliding blockage of the liquid guide hole 206 by the movable plug 207 and the effect of the air pressure inside the sampling tube 201, the reaction solution inside the sampling tube 201 continuously flows and mixes with the sample, improving the mixing degree of the reaction solution and the sample, and effectively improving the detection accuracy and detection stability.

[0076] When the distance sensor located above the 96-well antimicrobial susceptibility plate 4 detects that the distance between it and the floating plate 214 meets the set distance value, the controller controls the electric push rod 104 to start and drive the movable frame 105 to return to its original position. The movable frame 105 then drives the sampling tubes 201 on both sides to move upward and return to their original positions. The reaction solution inside the sampling tube 201 located above the ATP fluorescence detector 3 is mixed with the sample and is located in the optimal detection position. The ATP fluorescence detector 3 is turned on to perform fluorescence detection on the sample inside the sampling tube 201, thereby obtaining the required bacterial antimicrobial susceptibility detection data.

[0077] After the test is completed, the controller controls the electric push rod 104 to move the movable frame 105 upward to the initial height, and presses the elastic limit block 118 to disengage it from the limit groove 120. The movable frame 105 is rotated so that the sampling tube 201 originally located above the 96-well drug sensitivity plate 4 moves to above the ATP fluorescence detector 3, while another set of sampling tubes 201 moves to above the 96-well drug sensitivity plate 4. The sampling tube 201 is removed and replaced with a new sampling tube 201. The above process is repeated to perform bacterial drug sensitivity testing on multiple sets of sampling tubes 201, and multiple sets of test data are obtained. The required conclusion is obtained by comparing multiple sets of data.

[0078] This device effectively detects bacterial drug susceptibility in samples with high accuracy and good results. It also mixes and stirs the samples inside the 96-well drug susceptibility plate 4, preventing bacterial sedimentation due to prolonged static conditions. Furthermore, it accurately detects the sample quantity, ensuring the accuracy and stability of subsequent tests. When mixing the sample and reaction solution inside the sampling tube 201, it ensures efficient sample flow, avoiding problems such as difficulty in dispensing and poor mixing due to negative pressure. The up-and-down vibration of the movable plug 207 and floating plate 214 inside the sampling tube 201 further improves the thoroughness of sample dispensing and the cleanliness of the internal structure of the sampling tube 201, ensuring the mixing effect of the sample and reaction solution and the accuracy of subsequent tests.

[0079] Third Embodiment

[0080] A method for using a bacterial antimicrobial susceptibility testing kit based on the ATP luciferase method, comprising the following steps:

[0081] Step 1: Sample pretreatment. Take the sampling tube 201 in the sampling assembly 2 out of the refrigeration equipment and let it stand until it returns to room temperature. Then install it on the support assembly 1. During the installation process, ensure that the opening groove 203 of the sampling tube 201 is at the bottom and that the lysis solution in the sampling tube 201 does not leak. At the same time, place the 96-well antimicrobial susceptibility plate 4 containing the bacteria to be tested on top of the support assembly 1.

[0082] Step 2: Sampling. The controller moves the electric push rod 104 downward, inserting the bottom of the sampling tube 201 into the 96-well drug sensitivity plate 4. The top block 212 and the sliding block 218 press against each other and drive the movable frame 105 to rotate around the electric push rod 104. The sampling tube 201 then rotates back and forth inside the 96-well drug sensitivity plate 4 to stir. After stirring is completed, the sampling tube 201 continues to move downward, and the sample enters the sampling tube 201 through the opening groove 203 and mixes with the lysis solution in the sampling tube 201 to produce a lysis reaction.

[0083] Step 3: Catalytic reaction. By adjusting the position of sampling tube 201, sampling tube 201 moves above ATP fluorescence detector 3, and sampling tube 201 flips over. The position of movable plug 207 inside sampling tube 201 changes, and sampling tube 201 continues to move upward, causing the sealing block on the inner wall of ATP fluorescence detector 3 to press and contact with sliding block 218. Sliding block 218 moves along sleeve 217 and causes internal gas to exert force on the bottom of floating plate 214 through exhaust hole 221, intermediate channel 220 and impact hole 222. Floating plate 214 moves up and down inside sampling tube 201 and drives movable plug 207 to move up and down. The lysed sample is completely mixed with the reaction solution. Luciferase in the reaction solution catalyzes the reaction of luciferin with oxygen, causing the sample to emit fluorescence.

[0084] Step 4: Detection. The ATP fluorescence detector 3 continues to move upward, allowing the sampling tube 201 to be inserted into the detection port of the ATP fluorescence detector 3. The fluorescence intensity of the sample is detected by the ATP fluorescence detector 3, thereby realizing the drug sensitivity detection of the sample. During this process, another set of sampling components 2 and the 96-well drug sensitivity plate 4 are replaced and sampled to realize the simultaneous detection of multiple sets of samples.

[0085] By further defining the usage method of the bacterial drug susceptibility detection component, the sampling accuracy and detection efficiency of the sample are effectively improved, making it more adaptable and more stable.

[0086] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A bacterial drug susceptibility detection component based on the ATP luciferase method, characterized in that, It includes a support assembly, a sampling assembly, an ATP fluorescence detector, and a 96-well drug sensitivity plate, wherein the top of the inner wall of the ATP fluorescence detector is provided with a sealing block; The support assembly includes a base, a controller is provided on one side of the base, and an electric push rod is provided in the middle of the upper surface of the base; The sampling assembly includes a sampling tube with an open slot extending through its outer bottom end. A fixing frame is located in the middle of the inner side of the sampling tube. A movable plug is located below the fixing frame. A transmission rod is located at the bottom end of the movable plug. A pressure block is located at the bottom end of the transmission rod. A distance sensor is located at the end of the pressure block. A top block is located on the outer side of the pressure block. A fixing plate is located at the bottom end of the top block, and the fixing plate is fixedly mounted on the top of the ATP fluorescence detector. The sampling tube has multiple sets of sleeves on one side, and a sliding block is slidably connected inside the sleeve. The sampling tube has a middle channel inside, and one side of the multiple sets of sleeves is connected to the middle channel through an exhaust hole. A one-way exhaust valve is provided inside the exhaust hole. An impact hole is provided inside the movable plug. A floating plate is movably connected inside the sampling tube, and multiple sets of counterweight rods are provided at the bottom of the floating plate.

2. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 1, characterized in that: The sampling assembly is provided in two sets, which are respectively positioned above the ATP fluorescence detector and the 96-well drug sensitivity plate. The sampling assembly, the ATP fluorescence detector, and the 96-well drug sensitivity plate are all positioned above the support assembly.

3. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 1, characterized in that: The sleeve has a connecting spring on its side wall, and the other side of the connecting spring is fixedly connected to the side wall of the sliding block. The floating plate has a misalignment groove on one side, which matches the transmission rod. The middle channel matches the impact hole, and the top output end of the impact hole matches the bottom of the floating plate. The controller electrically controls each electrical component, and the distance sensor is used to detect the distance between itself and the floating plate.

4. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 1, characterized in that: The base has fixed rods at both ends on its upper surface, and a support seat at the top of each fixed rod. The ATP fluorescence detector and the 96-well drug sensitivity plate are respectively positioned above the two support seats. The top of the electric push rod is movably mounted on a movable frame via a bearing. Both ends of the movable frame are equipped with connecting rods. The bottom of each connecting rod is equipped with a "door"-shaped bracket. Movable seats are provided through the bottom of the inner walls on both sides of the bracket. A bushing is provided on the inner side of the movable seat, and a buffer spring is provided between the bushing and the movable seat.

5. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 4, characterized in that: One end of the movable seat is provided with a transmission bevel gear, the outer side of the transmission bevel gear is provided with a drive bevel gear, the top end of the drive bevel gear is provided with a transmission shaft, and the transmission shaft is located on the outer side of the bracket. The top end of the transmission shaft is provided with a transmission gear, the outer side of the transmission gear is provided with an arc-shaped rack, there are two arc-shaped racks, the top end of the arc-shaped rack is provided with a top plate, and the top plate is fixedly located on the top end of the electric push rod.

6. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 4, characterized in that: The electric push rod has a positioning post at its top, a slot on the outside of the positioning post, an elastic limiting block inside the slot, a return spring between the elastic limiting block and the slot, and one end of the elastic limiting block is a hemispherical structure. The positioning post has a positioning ring on its outside, and the positioning ring is fixedly set above the movable frame. The positioning ring has two limiting grooves on its inside that correspond to the bracket, and the limiting grooves correspond to the elastic limiting block.

7. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 4, characterized in that: The sampling tube is positioned above the base. Both sides of the sampling tube have a hexagonal shaft in the middle, and the bushing is adapted to the hexagonal shaft. The lower surface of the fixing frame has multiple liquid guide tubes. The outer bottom end of the liquid guide tube is surrounded by multiple liquid guide holes. The middle of the movable plug has multiple through grooves. The liquid guide tube is positioned inside the through groove, and the bottom end of the liquid guide tube is flush with the opening of the through groove.

8. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 1, characterized in that: The movable plug has a groove in the middle of its top end, and a compression spring is provided inside the groove. The top end of the compression spring is located on the lower surface of the fixed frame. The outer side of the pressure block is set as an arc-shaped structure, and the pressure block is located inside the opening groove.

9. The bacterial drug susceptibility detection component based on the ATP luciferase method according to claim 1, characterized in that: The sampling tube has a lysis solution at its bottom, with the lysis solution level below the opening groove. The sampling tube also has a reaction solution at its top, positioned above the movable plug.

10. A method of using a bacterial drug susceptibility detection kit based on the ATP luciferase method, wherein the method utilizes the bacterial drug susceptibility detection kit based on the ATP luciferase method as described in claim 9 to detect the drug susceptibility of bacteria, characterized in that, Includes the following steps: Step 1: Sample pretreatment. Remove the sampling tube from the refrigeration equipment and let it stand until it returns to room temperature. Then install it on the support assembly. During installation, ensure that the opening groove of the sampling tube is at the bottom and that the lysis solution in the sampling tube does not leak. At the same time, place the 96-well antimicrobial susceptibility plate containing the bacteria to be tested on top of the support assembly. Step 2: Sampling. The controller moves the electric push rod downwards, inserting the bottom of the sampling tube into the 96-well antimicrobial susceptibility plate. The top block and the sliding block press against each other and drive the movable frame to rotate around the electric push rod. The sampling tube then rotates back and forth inside the 96-well antimicrobial susceptibility plate to stir. After stirring is complete, the sampling tube continues to move downwards, and the sample enters the sampling tube through the opening groove and mixes with the lysis solution in the sampling tube to produce a lysis reaction. Step 3: Catalytic reaction. By adjusting the position of the sampling tube, the sampling tube moves above the ATP fluorescence detector. The sampling tube flips over, and the position of the movable plug inside the sampling tube changes. The sampling tube continues to move upward, causing the sealing block on the inner wall of the ATP fluorescence detector to press against the sliding block. The sliding block moves along the sleeve, causing the internal gas to exert a force on the bottom of the floating plate along the exhaust port, the middle channel, and the impact hole. The floating plate moves up and down inside the sampling tube, driving the movable plug to move up and down. The lysed sample is completely mixed with the reaction solution. The luciferase in the reaction solution catalyzes the reaction of luciferin with oxygen, causing the sample to emit fluorescence. Step 4: Detection. The ATP fluorescence detector continues to move upward, allowing the sampling tube to be inserted into the detection port of the ATP fluorescence detector. The fluorescence intensity of the sample is detected by the ATP fluorescence detector, thereby realizing the drug sensitivity detection of the sample. During this process, another set of the sampling components and the 96-well drug sensitivity plate are replaced and sampled to realize the simultaneous detection of multiple sets of samples.

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