Method for improving germination rate and seedling rate of sugarcane hybrid seeds

Abstract

The invention discloses a method for improving the germination rate and the seedling rate of sugarcane hybrid seeds. Comprising the following steps: priming: soaking sugarcane hybrid seeds in water for 6-24 hours, and then drying until the weight of the seeds is not reduced any more; and coating: carrying out coating treatment on the dried sugarcane hybrid seeds by adopting a seed coating agent to form coated seeds. According to the seed coating agent, the volume ratio of 4% metalaxyl-M fludioxonil to water is 1: 1, or the volume ratio of 11% metalaxyl-M fludioxonil to azoxystrobin is 1: 2, and the ratio of the seed coating agent to the sugarcane hybrid seeds is 0.5 mL: 1 g to 1.5 mL: 1 g. An ideal effective method for promoting germination and seedling formation of the sugarcane hybrid seeds is obtained by adopting a treatment technology of combining seed initiation with seed coating agent coating, the germination rate of the seeds is increased by more than 10%, the seedling formation rate is increased by more than 20%, seed mildew is reduced, and the germination and seedling culture period of the sugarcane seeds is greatly shortened.

Description

Technical Field

[0001] This invention belongs to the field of sugarcane seed treatment technology, specifically relating to a method for improving the germination rate and seedling rate of sugarcane hybrid seeds. Background Technology

[0002] Sugarcane (Saccharum officinarum L.) is an important economic crop, possessing the triple core value of an industrial raw material, an energy source, and a source of edible sugar. Therefore, the breeding of new sugarcane varieties with multiple excellent traits such as high yield, high sugar content, and stress resistance is urgently needed. Sexual hybridization breeding of sugarcane is the core method for breeding new sugarcane varieties by creating new variations through inter-varietal hybridization, and it is an important guarantee for promoting sugarcane variety iteration and improving the quality and efficiency of the sugarcane industry. However, the actual production and application of sugarcane hybrid seeds currently face multiple challenges: long breeding cycles, high input costs, cumbersome propagation processes, and generally low seed setting rates, incomplete seed development, and low seed vigor. Furthermore, sugarcane seeds are small in size with limited endosperm nutrient reserves, resulting in weak seedling growth and poor stress resistance after germination; the seed coat surface is covered with villi and residual anther tissue, making it easy to adsorb pollutants and pathogenic microorganisms, significantly increasing the risk of seed contamination during production, storage, transportation, and sowing. Under the combined influence of the aforementioned factors, sugarcane hybrid seeds typically exhibit low germination rates, susceptibility to infection, and low seedling survival rates. Particularly with prolonged seed storage, seed deterioration and viability decline become more pronounced, with aged seeds showing a sharp decrease in germination capacity, severely hindering the breeding process of high-yield, high-quality, and multi-resistant sugarcane genetic resources. However, the quantity of sugarcane hybrid seeds used is small, and their seed treatment requirements are far less than those of other major crops such as corn, soybeans, wheat, and peanuts. This has led to the neglect of the urgent need for pre-planting treatment of sugarcane hybrid seeds, resulting in a lack of efficient and systematic solutions. Therefore, researching and establishing an effective treatment technology to improve the germination and seedling survival rates of sugarcane hybrid seeds is of significant practical importance for the effective utilization of sugarcane hybrid seeds and ensuring the sustainable development of the sugarcane industry. Summary of the Invention

[0003] The purpose of this invention is to provide a method that promotes rapid germination of sugarcane hybrid seeds, reduces the infection rate during germination, and increases seedling survival rate. To achieve the above objectives, this invention adopts the following technical solution:

[0004] This invention provides a method for improving the germination rate and seedling rate of sugarcane hybrid seeds, comprising the following steps:

[0005] S1. Initiation: Soak sugarcane hybrid seeds in water for 6-24 hours, then dry them until the seed weight no longer decreases;

[0006] S2. Coating: The dried sugarcane hybrid seeds obtained in step S1 are coated with a seed coating agent to form coated seeds. The seed coating agent contains metalaxyl and fludioxonil.

[0007] Preferably, the seed coating agent contains metalaxyl, fludioxonil, and pyraclostrobin.

[0008] Preferably, the seed coating agent is 4% metalaxyl-mancozeb·fludioxonil or 11% metalaxyl-mancozeb·fludioxonil·pyraclostrobin.

[0009] Preferably, the volume ratio of the 4% metalaxyl-fludioxonil or 11% metalaxyl-fludioxonil-pyraclostrobin to water is 1:1 to 1:5.

[0010] Preferably, the seed dressing agent is 4% metalaxyl-fludioxonil to water in a volume ratio of 1:1 to 1:3, or 11% metalaxyl-fludioxonil to pyraclostrobin in a volume ratio of 1:1.5 to 1:5.

[0011] Preferably, the seed coating agent is 4% metalaxyl-fludioxonil to water in a volume ratio of 1:1 or 11% metalaxyl-fludioxonil to azoxystrobin in a volume ratio of 1:2.

[0012] Preferably, the ratio of the seed dressing agent to the sugarcane hybrid seed in step S2 is from 0.5 mL:1 g to 1.5 mL:1 g.

[0013] Preferably, the initiation temperature used in step S1 is 20-30°C.

[0014] Preferably, the drying process in step S1 involves spreading the seeds flat on a table to dry naturally or drying them quickly in a fume hood for 24-72 hours.

[0015] Preferably, the specific steps are as follows:

[0016] S1, sugarcane hybrid seeds were kept at room temperature;

[0017] S2. Completely immerse the selected sugarcane hybrid seeds in water, stir, and soak at 20-30℃ for 6-24 hours to obtain soaked sugarcane hybrid seeds.

[0018] S3. After absorbing the moisture from the sugarcane seeds treated in step S2 with absorbent paper, spread the seeds out separately on absorbent paper or a sieve to dry, or place them in a fume hood for ventilation and drying until the surface hairs of the seeds are fluffed up again and the seed weight no longer decreases, thus obtaining the fully induced sugarcane hybrid seeds.

[0019] S4. Place the sugarcane hybrid seeds treated in step S3 into a self-sealing bag and mix them evenly according to the ratio of seed coating agent volume to seed weight of 0.5 mL:1 g-1.5 mL:1 g; the seed coating agent is 4% metalaxyl-fludioxonil to water in a volume ratio of 1:1 or 11% metalaxyl-fludioxonil to pyraclostrobin in a volume ratio of 1:2.

[0020] S5. Spread the mixed seeds from step S4 evenly on paper or a table, and let the seed surface dry to obtain coated sugarcane hybrid seeds.

[0021] This invention provides a method for promoting the germination of sugarcane hybrid seeds and improving seedling survival rate. This method combines seed initiation with functional coating. Initiation treatment activates the physiological activity of seeds and improves the uniformity of germination. At the same time, coating treatment containing antibacterial components inhibits seed mold and reduces seedling diseases, thereby effectively solving the technical problems of low germination rate and difficulty in seedling survival of sugarcane hybrid seeds. In addition, the coating technology can effectively reduce villi, separate residual anthers, improve seed flowability, and enhance the convenience of sowing.

[0022] This invention fills the gap and deficiencies in existing pre-sowing treatment technologies for sugarcane hybrid seeds, providing an effective seed treatment scheme to promote germination and improve seedling survival rate. This provides core support for improving the efficiency of sugarcane hybrid seed breeding and promoting the sustainable development of the sugarcane industry. This invention innovatively combines seed priming technology with seed coating technology, enhancing the germination and seedling survival rates of sugarcane hybrid seeds through dual treatment. Addressing the problems of low vigor, slow germination, and weak stress resistance in sugarcane hybrid seeds, this invention first uses priming treatment technology during the seed priming process to allow the seeds to fully complete their pre-germination physiological preparations without breaking through the seed coat, keeping them in the germination preparation stage. This effectively activates internal seed metabolism, repairs cell membrane structure, and synchronizes the germination process, significantly improving germination rate, germination potential, and uniformity of germination. Meanwhile, addressing the physical issues of seed adhesion and susceptibility to pathogen infection caused by abundant hairs and residual anthers on the seed surface, this invention further introduces seed coating treatment to form a physical barrier on the seed surface. This effectively gathers the seed surface hairs and separates most of the residual anthers, significantly improving the seed's appearance and giving it good flowability and dispersibility, which is beneficial for precise control of sowing rate. Simultaneously, the fungicide in the seed coating agent effectively controls the infection and spread of pathogens carried by the seeds or in the environment, enhancing the resistance of seeds and seedlings to pathogen invasion, thereby reducing seedling mortality due to mold and disease. This synergistic treatment scheme, by combining the "internal activation" of initiation technology with the "external protection" of coating technology, systematically overcomes the physiological obstacles and disease threats that restrict the application of sugarcane hybrid seeds. The integrated initiation-coating process of this invention provides a systematic solution for the standardized treatment and efficient utilization of sugarcane hybrid seeds.

[0023] This invention, based on the physical properties and germination characteristics of sugarcane hybrid seeds, utilizes seed coating to reduce the fuzz on sugarcane hybrid seeds, making them easier to sow and breaking away from the traditional practice of direct sowing. The use of seed priming combined with seed coating technology provides an ideal and effective method for promoting the germination and seedling formation of sugarcane hybrid seeds, improving seed germination and seedling rates, reducing seed mold, and significantly shortening the germination and seedling cultivation cycle. This invention is highly operable, increasing seed germination rate by over 10%, seedling formation rate by over 20%, and significantly improving seed mobility, providing core support for ensuring the efficiency of sugarcane seedling breeding and the sustainable development of the sugarcane industry. Attached Figure Description

[0024] Figure 1 This shows the difference in growth between the 8691 hybrid sugarcane seed coating treatment group and the control group. The left image shows day 14 of the germination experiment, and the right image shows day 25 of the germination experiment. In each germination box, the left side represents the coated group (24 hours after initiation) and the right side represents the uncoated control group (CK).

[0025] Figure 2 This shows the difference in seed growth between the 8479 hybrid sugarcane seed coating treatment group and the control group. The left image shows day 14 of the germination experiment, and the right image shows day 25 of the germination experiment. In each germination box, the left side represents the coated group (24 hours after initiation) and the right side represents the uncoated control group (CK).

[0026] Figure 3 The image shows the fuzzy texture of sugarcane hybrid seeds after coating (left: uncoated seed, right: coated seed).

[0027] Figure 4 This shows the anther shedding of sugarcane hybrid seeds after coating (the left image shows the coated sugarcane hybrid seeds, and the right image shows the anthers that have fallen off after coating). Detailed Implementation

[0028] This method involves initiating and coating sugarcane hybrid seeds. Specifically, the seeds are first initiated with an initiation treatment, then coated with a fungicide. The resulting coated seeds can be used for germination or cultivation in ordinary substrates. Using the sugarcane hybrid seed treatment method provided by this invention, germination occurs earlier than untreated seeds, with a germination rate increased by more than 10% and a seedling survival rate increased by more than 40%. Germination is faster and more uniform, and the contamination rate of seedlings is controlled, significantly improving the seedling survival rate. When applied to the cultivation of sugarcane seedlings, this method can significantly improve the quantity and quality of sugarcane seedling populations of different hybrid combinations.

[0029] The following embodiments are further illustrations of the present invention, but not limitations thereof.

[0030] The 4% metalaxyl-mancozeb·fludioxonil (1.5% metalaxyl-mancozeb + 3.5% fludioxonil by mass) used in this invention was purchased from Shandong Zhaofengnian Biotechnology Co., Ltd., registration certificate: PD20190150; the 11% metalaxyl-mancozeb·fludioxonil·pyraclostrobin (3.3% fludioxonil + 1.1% pyraclostrobin + 6.6% pyraclostrobin by mass) was purchased from Shenzhen Noposion Crop Science Co., Ltd., registration certificate number: PD20161187.

[0031] Example 1: Treatment of 8691 sugarcane hybrid seeds

[0032] A method for improving the germination rate and seedling survival rate of 8691 sugarcane hybrid seeds, the steps of which are as follows:

[0033] 1) Seed collection: 8691 sugarcane hybrid seeds are stored in a low-temperature cold storage. Before processing, the seeds are taken out and placed at room temperature to dry for later use.

[0034] 2) Soaking seeds: Weigh 4 g of sugarcane hybrid seeds and divide them into two groups. Soak them in 100 mL of clean water and stir with a glass rod to ensure that all seeds are submerged in water. Soak them at room temperature for 6 hours and 24 hours respectively to obtain induced sugarcane hybrid seeds.

[0035] 3) Drying: After step 2), use absorbent paper to absorb excess moisture from the seeds, spread them out on paper or in a container, and place them in a fume hood to air dry until the seed surface is completely dry and the seeds are fluffy.

[0036] 4) Coating: Mix 2 mL of 4% metalaxyl-methyl·fludioxonil with 2 mL of water to make a diluted solution. Take 2 g of the sugarcane hybrid seeds treated in step 3) and place them in a resealable bag. Use a pipette to take 1.5 mL of the diluted solution and quickly and evenly sprinkle it on the seeds. Blow air into the resealable bag until it is full, then seal the bag. Shake vigorously to mix most of the seeds with the diluted solution evenly and until the fuzz on the seed surface shrinks. Take out the seeds and spread them out to dry.

[0037] 5) Germination Test: The seeds from step 4) were placed in germination boxes lined with three layers of moist germination paper. The germination boxes measured 12 cm × 12 cm × 9 cm. Each treatment was repeated three times, with 100 seeds per replicate. Germination was carried out at 25℃. Water was added as needed based on the dryness of the germination paper to ensure the paper was moist but without surface water. Germination potential and germination rate were recorded on days 7 and 14, respectively. Germination was defined as the embryo breaking through the seed coat and reaching half the seed length. Germination potential was recorded on day 7, and germination rate was recorded on day 14. Mold rate and seedling survival rate were recorded on day 25. Seedling survival was defined as seedlings with a root length and height of 1 cm or more without rot or mold.

[0038] In Table 1, the treatment for the CK group (control group) was as follows: after completing step 1) seed collection, proceed directly to step 5) germination test. The treatment for the 6-hour germination group and the 24-hour germination group was as follows: after completing step 3) re-drying, proceed directly to step 5) germination test. The treatment for the coated group was as follows: after completing step 1) seed collection, proceed directly to step 4) coating and subsequent step 5) germination test.

[0039] The results of this embodiment ( Figure 1 Table 1 shows that the method of initiation followed by coating treatment can synergistically enhance and significantly improve the germination speed (germination potential), final germination ability (germination rate), and seedling survival rate (seedling survival rate) of 8691 sugarcane hybrid seeds, while effectively inhibiting mold growth (mold rate) during germination. Overall, the promoting effect of initiation treatment is significantly better than that of no initiation treatment, and the effect of 24-hour initiation treatment is better than that of 6-hour initiation treatment. Under the same initiation time, the coating treatment is better than the uncoated treatment. In terms of germination indicators, the germination potential and germination rate of 24-hour initiation + coating are the highest, reaching 36.3% and 39.3% respectively, significantly higher than other treatments, indicating that the present invention can effectively accelerate the seed germination process and increase the proportion of germinated seeds. In terms of mold rate, all coated treatments were effectively controlled below 1%, significantly lower than the uncoated treatment group, indicating that coating has a significant effect on reducing mold infection of sugarcane hybrid seeds. Regarding the seedling survival rate, the 24-hour initiation + coating treatment achieved 38.7%, significantly better than the 24-hour initiation treatment, while the 6-hour initiation + coating treatment achieved 32.3%, also significantly better than the 6-hour initiation treatment. Overall, the seed germination rate of the coating treatment alone was not significantly different from the control (CK), but the seedling survival rate was significantly improved. This indicates that coating alone cannot significantly improve the seed germination rate, but it can play a good protective role in the later stages and promote seedling survival. Although the initiation treatment alone can significantly improve the germination potential and germination rate, the seedling survival rate will decrease due to pathogen infection in the later stages. The initiation + coating treatment can achieve a good comprehensive improvement in germination potential, germination rate, and seedling survival rate, achieving synergistic effects.

[0040] Example 2: Coating treatment initiated by 8479 sugarcane hybrid seeds

[0041] A method for improving the germination rate and seedling survival rate of 8479 sugarcane hybrid seeds, the steps of which are as follows:

[0042] 1) Seed collection: 8479 sugarcane hybrid seeds are stored in a low-temperature cold storage. Before processing, the seeds are taken out and placed at room temperature to dry for later use.

[0043] 2) Soaking seeds: Weigh 4 g of sugarcane hybrid seeds and divide them into two groups. Soak them in 100 mL of clean water and stir with a glass rod to ensure that all seeds are submerged in water. Soak them at 25℃ for 6 hours and 24 hours respectively to obtain the induced sugarcane hybrid seeds.

[0044] 3) Drying: After step 2), use absorbent paper to absorb excess moisture from the seeds, spread them out on paper or in a container, and place them in a fume hood to air dry until the seed surface is completely dry and the seeds are fluffy.

[0045] 4) Coating: Mix 2 mL of 11% metalaxyl-mancozeb-fludioxonil-pyraclostrobin with 4 mL of water to make a diluted solution. First, place 1 g of sugarcane hybrid seeds treated in step 3) in a resealable bag. Use a pipette to take 1 mL of the diluted solution and quickly and evenly sprinkle it on 1 g of seeds. Blow air into the resealable bag until it is full, then seal the bag. Shake vigorously to mix most of the seeds with the diluted solution evenly and until the fuzz on the seed surface shrinks. Take out the seeds and spread them out to dry.

[0046] 5) Germination Test: The seeds from step 4) were placed in germination boxes lined with three layers of moist germination paper. The germination boxes measured 12 cm × 12 cm × 9 cm. Each treatment was repeated three times, with 100 seeds per replicate. Germination was carried out at 25℃. Water was added as needed based on the dryness of the germination paper to ensure the paper was moist but without surface water. Germination potential and germination rate were recorded on days 7 and 14, respectively. Germination was defined as the embryo breaking through the seed coat and reaching half the seed length. Germination potential was recorded on day 7, and germination rate was recorded on day 14. Mold rate and seedling survival rate were recorded on day 25. Seedling survival was defined as seedlings with a root length and height of 1 cm or more without rot or mold.

[0047] In Table 2, the treatment for the CK group (control group) was as follows: after completing step 1) seed collection, proceed directly to step 5) germination test. The treatment for the 6-hour germination group and the 24-hour germination group was as follows: after completing step 3) re-drying, proceed directly to step 5) germination test. The treatment for the coated group was as follows: after completing step 1) seed collection, proceed directly to step 4) coating and subsequent step 5) germination test.

[0048] The results of this embodiment ( Figure 2Table 2 shows that seed coating played a decisive role in preventing mold and fungi and improving seedling survival rate. Regardless of the initiation time, the mold rate of all coated treatment groups (coated, 6 hours initiation + coating, 24 hours initiation + coating) was consistently controlled below 1.0%, which was significantly different from the 4.0%-5.7% mold rate of the uncoated groups. This indicates that seed coating provides a crucial healthy environment for seed germination and seedling growth, laying the foundation for the final seedling quantity. Initiation treatment is the key factor in improving seed germination rate, accelerating the initiation of internal physiological and biochemical activities of the seed, speeding up seed germination (increasing germination potential) and improving the final germination rate. Among them, the germination potential of the pre-treated seeds after 24 hours of initiation reached 16.0%, significantly higher than that of the seeds treated after 6 hours of initiation (11.3%) and the untreated control group (6.3%). Coating effectively resists pathogen infection, while initiation treatment fully activates the physiological potential of seeds from within. In this example, the combined treatment (24 hours of initiation + coating) resulted in a germination potential of 18.3%, a germination rate of 24.3%, and a seedling survival rate as high as 23.3%, all of which were the highest values ​​among all treatments. Compared to the control group's 12.0% seedling survival rate, this represents a significant improvement of 94%. These results demonstrate that the synergistic effect of initiation and coating can systematically solve industry problems such as uneven germination, low seedling survival rate, and susceptibility to infection in sugarcane hybrid seeds, providing a stable and reliable technical solution for achieving efficient seedling cultivation of sugarcane hybrid seeds.

[0049] Example 3: Sugarcane Hybrid Seed Coating Technology

[0050] A method for improving the germination rate and seedling survival rate of sugarcane hybrid seeds, the steps of which are as follows:

[0051] 1) Seed collection: Sugarcane hybrid seeds are stored in a low-temperature cold storage. Before processing, the seeds are taken out and placed at room temperature to dry for later use.

[0052] 2) Coating: Mix 2 mL of 4% metalaxyl-methyl·fludioxonil with 2 mL of water to make a diluted solution. Place 2 g of sugarcane hybrid seeds in a resealable bag. Use a pipette to quickly and evenly sprinkle 2 mL of the diluted solution onto the 2 g of seeds. Blow air into the resealable bag until it is full, then seal the bag. Shake vigorously to mix most of the seeds with the diluted solution evenly and until the fuzz on the seed surface shrinks. Remove the seeds and spread them out to dry to make coated seeds.

[0053] Sugarcane is hermaphroditic, and hybrid sugarcane seeds have feathery, forked residual stigmas and several residual purplish-red anthers (usually 3-4 if they haven't fallen off). The base of the seed also has many filamentous hairs. These characteristics make sugarcane seeds highly susceptible to carrying or harboring impurities and pathogens. In this invention, the seed coating process requires shaking and thoroughly mixing the seeds with a diluted seed coating agent. According to the mixing ratio of this patent, the water and film-forming agent in the seed coating agent allow the filaments of individual hybrid sugarcane seeds to adhere together, thus achieving a binding effect. Furthermore, the seeds themselves or the seeds and the self-sealing bag will not stick together, allowing the seeds to maintain their shape. Figure 3 The image on the right shows the seeds in a loose, separated state. Simultaneously, the contraction of the seed villi facilitates the exposure of the anthers. Through friction between the seeds or between the seeds and the resealable bag, most of the remaining anthers on the seeds can be detached. Observation of the remaining anthers on the seed surface shows that approximately 3-4 anthers on each seed can be completely detached, or only one remains. Based on this, the detachment rate can reach over 75%. Figure 3 , 4 This significantly reduces the probability of pathogenic microorganisms attaching to and infecting seeds. In summary, this patent innovatively applies coating technology to the treatment of sugarcane hybrid seeds, which can not only effectively inhibit pathogenic microorganisms carried by the seeds, but also effectively shrink the villi on the surface of sugarcane hybrid seeds and significantly remove attached anthers, significantly improving the seed's dispersibility and flowability, which is conducive to achieving precise and uniform sowing, and effectively improving sowing efficiency and seedling quality.

[0054] Table 1 Germination indices of seeds from the 8691 sugarcane hybrid combination under different treatments.

[0055] Table 2 Germination indices of seeds from the 8479 sugarcane hybrid combination under different treatments

Claims

1. A method for improving the germination rate and seedling rate of hybrid sugarcane seeds, characterized in that, Includes the following steps: S1. Initiation: Soak sugarcane hybrid seeds in water for 6-24 hours, then dry them until the seed weight no longer decreases; S2. Coating: The dried sugarcane hybrid seeds obtained in step S1 are coated with a seed coating agent to form coated seeds. The seed coating agent contains metalaxyl and fludioxonil.

2. The method according to claim 1, characterized in that, The seed coating agent contains metalaxyl, fludioxonil, and pyraclostrobin.

3. The method according to claim 1, characterized in that, The seed dressing agent is 4% metalaxyl-mancozeb·fludioxonil or 11% metalaxyl-mancozeb·fludioxonil·pyraclostrobin.

4. The method according to claim 3, characterized in that, The volume ratio of the 4% metalaxyl-fludioxonil or 11% metalaxyl-fludioxonil-pyraclostrobin to water is 1:1 to 1:

5.

5. The method according to claim 4, characterized in that, The seed dressing agent is 4% metalaxyl-mancozeb·fludioxonil and water in a volume ratio of 1:1-1:3, or 11% metalaxyl-mancozeb·fludioxonil·pyraclostrobin and water in a volume ratio of 1:1.5-1:

5.

6. The method according to claim 5, characterized in that, The seed coating agent is 4% metalaxyl-fludioxonil and water in a volume ratio of 1:1 or 11% metalaxyl-fludioxonil and pyraclostrobin in a volume ratio of 1:

2.

7. The method according to claim 1, characterized in that, The ratio of seed dressing agent to sugarcane hybrid seeds in step S2 is from 0.5 mL:1 g to 1.5 mL:1 g.

8. The method according to claim 1, characterized in that, The initiation temperature used in step S1 is 20-30℃.

9. The method according to claim 1, characterized in that, The drying process described in step S1 involves spreading the seeds flat on a work surface to dry naturally or quickly drying them in a fume hood for 24-72 hours.

10. The method according to claim 1, characterized in that, The specific steps are as follows: S1, sugarcane hybrid seeds were kept at room temperature; S2. Completely immerse the selected sugarcane hybrid seeds in water, stir, and soak at 20-30℃ for 6-24 hours to obtain soaked sugarcane hybrid seeds. S3. After absorbing the moisture from the sugarcane hybrid seeds treated in step S2 with absorbent paper, spread the seeds out separately on absorbent paper or a sieve to dry, or place them in a fume hood for ventilation and drying until the surface hairs of the seeds are fluffed up again and the seed weight no longer decreases, thus obtaining the fully induced sugarcane hybrid seeds. S4. Place the sugarcane hybrid seeds treated in step S3 into a self-sealing bag and mix them evenly according to the ratio of seed coating agent volume to seed weight of 0.5 mL:1 g-1.5 mL:1 g; the seed coating agent is 4% metalaxyl-fludioxonil to water in a volume ratio of 1:1 or 11% metalaxyl-fludioxonil to pyraclostrobin in a volume ratio of 1:

2. S5. Spread the mixed seeds from step S4 evenly on paper or a table, and let the seed surface dry to obtain coated sugarcane hybrid seeds.

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