Device and method for preparing frozen diluted boar semen

Abstract

The invention provides a preparation device and method of frozen diluted semen of pigs, and relates to the technical field of animal husbandry engineering.The preparation device of the frozen diluted semen of the pigs comprises a rack; the shaking mechanism comprises a shaking table, a swinging frame, a rotating arm and a shaking motor, the swinging frame is hinged to the rack, the shaking table is hinged to the swinging frame, a connecting rod extending downwards is arranged at the bottom of the shaking table, the shaking motor is fixedly arranged on the rack, the rotating arm is connected with an output shaft of the shaking motor, a ball head sleeve is arranged on the rotating arm, and a ball head is arranged on the connecting rod; the ball head is spherically hinged with the ball head sleeve; the first heater is arranged on the shaking table; and the dilution cup is detachably connected with the first heater. The shaking mechanism drives the diluent and the fresh semen in the diluting cup to be mildly mixed and diluted, the whole liquid flows mildly, mixing is milder and more uniform, the structure and activity of the semen are protected physiologically, and meanwhile the device has the practical operation advantages of being convenient to operate and free of equipment pollution risk.

Description

Technical Field

[0001] This application relates to the field of animal husbandry engineering technology, and in particular to an apparatus and method for preparing frozen diluted bovine semen. Background Technology

[0002] Frozen diluted boar semen is a product made from fresh semen of healthy boars. After standardized processes including quality testing, dilution protection, low-temperature equilibration, and programmed freezing, it is preserved in liquid nitrogen for long-term storage. It is a core technology product in the field of artificial insemination of pigs and an important way to preserve the superior genetic traits of boars. Frozen diluted boar semen can be stored for several years in liquid nitrogen, breaking the time limit of fresh semen which can only be stored at room temperature for a few days. It also solves the problems of short transportation distances and susceptibility to temperature changes in fresh semen, enabling the cross-regional and long-term dissemination of superior boar genes. Furthermore, semen from a single superior boar can be produced in large quantities through freezing, significantly improving boar breeding efficiency, reducing boar feeding costs on pig farms, and accurately selecting high-quality breeding boars to optimize the genetic performance of pig breeds.

[0003] The preparation method of frozen diluted boar semen includes steps such as preparing the diluent, receiving fresh semen, diluting the fresh semen, filling and sealing with capillary tubes, programmed freezing, thawing and testing the frozen semen, and storing in a liquid nitrogen tank. In the fresh semen dilution step, the prepared diluent is typically heated to a certain temperature first, and then the collected fresh semen is poured into the diluent and stirred. For example, utility model patent CN215080250U discloses a constant-temperature dilution device for boar semen. This device includes an insulated stainless steel tank, an automatic stirrer, a stirring rod, stirring blades, a heating rod mechanism, and a temperature controller. In use, the diluent powder is dissolved in the insulated stainless steel tank, heated by the heating rod mechanism, and the temperature is controlled by the temperature controller to achieve constant-temperature heating. When the fresh semen is poured into the insulated stainless steel tank, the stirring blades on the stirring rod are driven by the automatic stirrer to rapidly stir, thus diluting the fresh semen.

[0004] However, the aforementioned constant-temperature dilution device for boar semen uses stirring blades to agitate the diluent and fresh semen during dilution. Boar sperm cell membranes are thin and fragile, extremely sensitive to mechanical forces, shear forces, and solution disturbances. The stirring method can easily cause cell membrane rupture, resulting in physical damage to the sperm and loss of fertilization capacity, thus affecting the quality of the final frozen diluted boar semen. Although some dilution devices on the market use magnetic stirrers, improper speed control can still cause mechanical shear forces generated by the stirrer, easily damaging sperm structure and hindering the improvement of the quality of the final frozen diluted boar semen product. Summary of the Invention

[0005] The purpose of this application is to provide an apparatus and method for preparing frozen diluted boar semen, which solves the problem that in related technologies, constant temperature dilution devices for boar semen can easily cause physical damage to boar sperm when diluting fresh semen, thus affecting the quality of the final frozen diluted boar semen.

[0006] Firstly, the apparatus for preparing frozen diluted bovine semen provided in this application adopts the following technical solution:

[0007] An apparatus for preparing frozen diluted bovine semen, comprising:

[0008] frame;

[0009] A rocking mechanism includes a rocking table, a swing frame, a rotary arm, and a rocking motor. The swing frame is hinged to the frame via a first hinge shaft, and the rocking table is hinged to the swing frame via a second hinge shaft, with the axes of the first and second hinge shafts perpendicular to each other. A downwardly extending connecting rod is provided at the bottom of the rocking table. The rocking motor is fixed to the frame, and the rotary arm is connected to the output shaft of the rocking motor. A ball head sleeve is provided on the rotary arm, and a ball head is provided on the connecting rod. The ball head is spherically hinged to the ball head sleeve.

[0010] A first heater is disposed on the shaking table;

[0011] A dilution cup, which is detachably connected to a first heater for heating the dilution cup.

[0012] Optionally, it also includes a semen storage cylinder, a valve assembly, a lifting seat, and a second heater. The lifting seat is slidably mounted vertically on the frame, and the frame is provided with a lifting mechanism for driving the lifting seat to move vertically. The second heater is mounted on the lifting seat, and the semen storage cylinder is connected to the second heater and located above the dilution cup. The second heater is used to heat the semen storage cylinder. The bottom of the semen storage cylinder is provided with a semen discharge nozzle, and the valve assembly is used to control the opening and closing of the semen discharge nozzle.

[0013] Optionally, a clamping component is also included. The first heater includes a first housing, which is fixed to the shaking table. The first housing has a cavity for accommodating a dilution cup. The first housing is filled with a heat-conducting liquid. The first housing has a first temperature control unit for controlling the temperature of the heat-conducting liquid in the first housing. The dilution cup is placed in the cavity. The top of the dilution cup has a limiting plate that can abut against the upper surface of the first housing. The clamping component includes a pressure plate and a clamping bolt. The first housing has a vertical column and a screw hole. The pressure plate is slidably sleeved on the vertical column. The pressure plate has a through hole. The clamping bolt passes through the through hole and is screwed to the first housing through the screw hole. The pressure plate has a pad for abutting against the limiting plate.

[0014] Optionally, the second heater includes a second housing, which is fixedly mounted on the lifting seat. The semen storage cylinder is fixedly inserted through the second housing in a vertical direction. The second housing is filled with a heat-conducting liquid, and a second temperature control unit is provided on the second housing for controlling the temperature of the heat-conducting liquid inside the second housing.

[0015] Optionally, the valve assembly includes a valve core, a closing spring, and a linkage assembly. The semen storage cylinder is provided with a valve cavity communicating with the semen discharge nozzle. The semen storage cylinder is also provided with a channel communicating with the valve cavity and the interior of the semen storage cylinder. The valve core is located in the valve cavity and is used to open and close the connection between the valve cavity and the channel. The closing spring acts on the valve core and is used to push the valve core to close. The rocking motor is connected to the valve core through the linkage assembly and is used to drive the valve core to open intermittently.

[0016] Optionally, the semen storage cylinder is provided with a limiting frame, the valve core is provided with a valve stem, the valve stem slides through the semen storage cylinder and the limiting frame, the valve stem is provided with a baffle, and the valve closing spring is sleeved on the outside of the valve stem, with the two ends of the valve closing spring abutting against the limiting frame and the baffle respectively.

[0017] Optionally, the linkage assembly includes a lever, a roller, a rack, an incomplete gear, and a return spring. The lever is hinged to the semen storage cylinder and has a sliding groove. The valve stem has a pin that passes through the sliding groove. The roller is rotatably mounted on the end of the lever. The rack is slidably mounted on the semen storage cylinder. The incomplete gear is rotatably mounted on the semen storage cylinder. The shaking motor is connected to the incomplete gear through a transmission assembly and is used to drive the incomplete gear to rotate. The incomplete gear can intermittently mesh with the rack to intermittently push the rack to slide in the direction of contact with the roller. The return spring acts on the rack and is used to push the rack to slide in the direction of separation from the roller.

[0018] Optionally, the semen storage cylinder is provided with a stop block, the rack is provided with a column, the column is slidably inserted through the stop block, the end of the column away from the rack is provided with a flange, the return spring is sleeved on the outside of the column, and the two ends of the return spring abut against the stop block and the flange respectively.

[0019] Optionally, the transmission assembly includes a secondary transmission gear, a drive shaft, and a driven shaft. The secondary transmission gear is rotatably mounted on the frame. A rotating shaft is provided on the rotary arm. The rotating shaft is fixedly connected to the output shaft of the rocking motor. A primary transmission gear meshing with the secondary transmission gear is provided on the rotating shaft. The drive shaft is rotatably mounted on the frame in a vertical direction. The drive shaft is connected to the secondary transmission gear through a first transmission shaft. A square hole is provided on the drive shaft in an axial direction. The driven shaft is rotatably mounted on the lifting seat in a vertical direction. A square rod is provided on the driven shaft in an axial direction. The square rod is slidably inserted into the square hole of the drive shaft. The driven shaft is connected to the incomplete gear through a second transmission shaft.

[0020] Secondly, the method for preparing frozen diluted bovine semen provided in this application adopts the following technical solution:

[0021] A method for preparing frozen diluted boar semen, based on the aforementioned apparatus for preparing frozen diluted boar semen, includes the following steps:

[0022] S1. Pour the prepared diluent into the dilution cup, heat the diluent in the dilution cup with the first heater, then drive the rotary arm to rotate by the shaking motor, drive the shaking table to shake, and drive the first heater and the dilution cup to shake. At the same time, pour the collected fresh essence into the diluent for mixing and diluting the fresh essence.

[0023] S2. Remove the dilution cup from the first heater, seal the diluted semen, and then place it in a constant temperature incubator for equilibration.

[0024] S3. Fill the balanced semen into plastic capillary tubes using a capillary tube filling machine, and seal the tubes immediately after filling using a sealing machine;

[0025] S4. Place the sealed thin tube neatly into the programmable freezer, and set the freezing curve after placing it into the instrument;

[0026] S5. Randomly sample and thaw frozen semen for testing. If the test is qualified, transfer the frozen semen to a liquid nitrogen tank for storage.

[0027] In summary, this application includes at least the following beneficial technical effects: In the method for preparing frozen diluted bovine semen of this application, the prepared diluent is poured into a dilution cup, and the diluent in the dilution cup is heated by a first heater. Then, a rotating arm is driven by a shaking motor to rotate, which in turn drives the shaking table to shake, causing the first heater and the dilution cup to shake. At the same time, the collected fresh semen is poured into the diluent for mixing, thus diluting the fresh semen. Because the diluent and fresh semen in the dilution cup are gently mixed and diluted by the shaking mechanism during the dilution process, the gentle overall liquid flow replaces the mechanical damage to the sperm caused by the stirring blades in the prior art. This makes the mixing gentler and more uniform, adapting to the low-stress operation requirements of semen dilution. It not only protects the structure and motility of sperm from a physiological perspective, but also has the practical advantages of convenient operation and no risk of equipment contamination. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the apparatus for preparing frozen and diluted bovine semen in an embodiment of this application;

[0029] Figure 2 for Figure 1 A magnified view of part A2 in the middle;

[0030] Figure 3 for Figure 1 A magnified view of part A3 in the middle;

[0031] Figure 4 This is a first-view cross-sectional view of the apparatus for preparing frozen diluted bovine semen in an embodiment of this application;

[0032] Figure 5 for Figure 4 A magnified view of part B in the middle section;

[0033] Figure 6 for Figure 1 A magnified view of part A1 in the diagram;

[0034] Figure 7 for Figure 5 A magnified view of part B1 in the middle;

[0035] Figure 8 This is a cross-sectional view from a second perspective of the apparatus for preparing frozen and diluted bovine semen in an embodiment of this application.

[0036] Figure 9 for Figure 8 A magnified view of part C in the middle;

[0037] Figure 10 for Figure 9 Cross-sectional view of the valve assembly.

[0038] Explanation of reference numerals in the attached figures:

[0039] 10. Frame; 11. First hinge shaft; 12. Guide column; 20. Shaking mechanism; 21. Shaking table; 211. Second hinge shaft; 212. Connecting rod; 213. Ball head; 22. Swing frame; 23. Rotating arm; 231. Ball head sleeve; 232. Rotating shaft; 233. Main transmission gear; 24. Shaking motor;

[0040] 30. First heater; 31. First housing; 311. Cavity; 312. Vertical column; 313. Screw hole; 32. First heating element; 33. First temperature sensor; 34. First temperature controller; 40. Diluting cup; 41. Limiting plate;

[0041] 50. Semen storage cylinder; 51. Semen discharge nozzle; 52. Valve chamber; 53. Channel; 54. Limiting bracket; 55. Valve stem hole; 56. Sealing ring; 57. Bracket; 58. Guide sleeve; 59. Stop block;

[0042] 60. Valve assembly; 61. Valve core; 611. Sealing gasket; 612. Valve stem; 613. Baffle; 614. Pin; 62. Valve closing spring; 63. Lever; 631. Pivot; 632. Slide groove; 64. Roller; 65. Rack; 651. Column; 652. Flange; 66. Incomplete gear; 67. Return spring; 68. Transmission assembly; 681. Secondary transmission gear; 682. Drive shaft; 683. Driven shaft; 6831. Square rod; 684. First transmission shaft; 685. Second transmission shaft; 686. First bevel gear pair; 687. Second bevel gear pair; 688. Third bevel gear pair; 689. Fourth bevel gear pair;

[0043] 70. Lifting seat; 71. Screw sleeve; 80. Second heater; 81. Second housing; 82. Second heating element; 83. Second temperature sensor; 84. Second temperature controller; 90. Clamping element; 91. Pressure plate; 911. Through hole; 92. Clamping bolt; 93. Pad; 100. Screw. Detailed Implementation

[0044] The following is in conjunction with the appendix Figure 1 -Appendix Figure 10 This application will be described in further detail below.

[0045] This application discloses an apparatus for preparing frozen diluted bovine semen.

[0046] An apparatus and method for preparing frozen diluted bovine semen includes a frame 10, a shaking mechanism 20, a first heater 30, a dilution cup 40, a semen storage cylinder 50, a valve assembly 60, a lifting seat 70, a second heater 80, and a clamping component 90.

[0047] Reference Figures 1 to 5 The rocking mechanism 20 includes a rocking table 21, a swing frame 22, a rotating arm 23, and a rocking motor 24. The swing frame 22 is hinged to the frame 10 via a first hinge shaft 11, and the rocking table 21 is hinged to the swing frame 22 via a second hinge shaft 211, with the axes of the first hinge shaft 11 and the second hinge shaft 211 perpendicular to each other. A downwardly extending connecting rod 212 is provided at the bottom of the rocking table 21. The rocking motor 24 is fixedly mounted on the frame 10, and a rotating shaft 232 is provided on the rotating arm 23. The rotating shaft 232 is fixedly connected to the output shaft of the rocking motor 24, thereby connecting the rotating arm 23 to the output shaft of the rocking motor 24. The rocking motor 24 can drive the rotating arm 23 to rotate. A ball head sleeve 231 is provided on the rotating arm 23, and a ball head 213 is provided on the connecting rod 212. The ball head 213 is spherically hinged to the ball head sleeve 231.

[0048] The first heater 30 is mounted on the shaking table 21. The dilution cup 40 is detachably connected to the first heater 30, and the first heater 30 is used to heat the dilution cup 40. The specific structure of the first heater 30, and the specific connection relationship between the shaking table 21, the first heater 30, and the dilution cup 40 are as follows:

[0049] The first heater 30 includes a first housing 31, which is fixed on the shaking table 21. The first housing 31 is filled with a heat-conducting liquid, and a first temperature control unit is provided on the first housing 31 for controlling the temperature of the heat-conducting liquid inside the first housing 31. The first temperature control unit includes a first heating element 32, a first temperature sensor 33, and a first temperature controller 34. The first heating element 32 is fixed on the first housing 31 and is used to heat the heat-conducting liquid inside the first housing 31. The first heating element 32 can be an encapsulated electric heating tube. The first temperature sensor 33 is fixed on the first housing 31 and is used to monitor the temperature of the heat-conducting liquid inside the first housing 31. The first temperature controller 34 is fixed on the first housing 31. The first heating element 32 and the first temperature sensor 33 are electrically connected to the first temperature controller 34. The specific electrical connection relationship between the first heating element 32, the first temperature sensor 33, and the first temperature controller 34 is prior art in the art and can be implemented by those skilled in the art, and will not be described in detail here.

[0050] The first housing 31 has a cavity 311 for accommodating the dilution cup 40. The dilution cup 40 is placed in the cavity 311. The top of the dilution cup 40 is provided with a limiting plate 41 that can abut against the upper surface of the first housing 31. The clamping member 90 includes a pressure plate 91 and a clamping bolt 92. The first housing 31 has a vertical post 312 and a screw hole 313. The pressure plate 91 is slidably sleeved on the vertical post 312. The pressure plate 91 has a through hole 911. The clamping bolt 92 passes through the through hole 911 and is screwed to the first housing 31 through the screw hole 313. The pressure plate 91 has a pad 93 for abutting against the limiting plate 41. After tightening the clamping bolt 92, the pad 93 can be pressed tightly against the upper surface of the limiting plate 41, thereby fixing the dilution cup 40 on the first housing 31.

[0051] Reference Figure 1 , Figure 3 , Figures 6 to 10 The frame 10 is provided with guide column 12, and the lifting seat 70 is slidably sleeved on the guide column 12 of the frame 10. The frame 10 is provided with a lifting mechanism for driving the lifting seat 70 to move vertically. The lifting mechanism includes screw 100, and the lifting seat 70 is provided with screw sleeve 71. The screw 100 is rotatably mounted on the frame 10 and screwed to the screw sleeve 71.

[0052] The second heater 80 is mounted on the lifting base 70. The semen storage cylinder 50 is connected to the second heater 80 and located above the dilution cup 40. The second heater 80 is used to heat the semen storage cylinder 50. The specific structure of the second heater 80 and the specific connection relationship between the lifting base 70, the second heater 80 and the semen storage cylinder 50 are as follows: The second heater 80 includes a second housing 81, which is fixedly mounted on the lifting base 70. The semen storage cylinder 50 is fixedly inserted vertically through the second housing 81. The second housing 81 is filled with a heat-conducting liquid. A second temperature control unit is provided on the second housing 81 for controlling the temperature of the heat-conducting liquid inside the second housing 81. The second temperature control unit includes a second heating element 82, a second temperature sensor 83, and a second temperature controller 84. The second heating element 82 is fixed on the second housing 81 and is used to heat the heat-conducting liquid inside the second housing 81. The second heating element 82 may be an encapsulated electric heating tube. The second temperature sensor 83 is fixed on the second housing 81 and is used to monitor the temperature of the heat-conducting liquid inside the second housing 81. The second temperature controller 84 is fixed on the second housing 81. The second heating element 82 and the second temperature sensor 83 are electrically connected to the second temperature controller 84. The specific electrical connection relationship between the second heating element 82, the second temperature sensor 83, and the second temperature controller 84 is prior art in the art and can be implemented by those skilled in the art, and will not be described in detail here.

[0053] The semen storage cylinder 50 is provided with a semen discharge nozzle 51 at the bottom. The valve assembly 60 is used to control the opening and closing of the semen discharge nozzle 51. The valve assembly 60 includes a valve core 61, a valve closing spring 62 and a linkage assembly. The semen storage cylinder 50 is provided with a valve cavity 52 that communicates with the semen discharge nozzle 51. The semen storage cylinder 50 is also provided with a channel 53 that communicates with the valve cavity 52 and the interior of the semen storage cylinder 50. The valve core 61 is located in the valve cavity 52 and is used to open and close to seal the connection between the valve cavity 52 and the channel 53. The valve core 61 is provided with a sealing gasket 611 that can abut against the inner wall of the connection between the valve cavity 52 and the channel 53.

[0054] The semen storage cylinder 50 is equipped with a limiting frame 54, the valve core 61 is equipped with a valve stem 612, and the semen storage cylinder 50 is equipped with a valve stem hole 55. The valve stem 612 slides through the valve stem hole 55 and the limiting frame 54 of the semen storage cylinder 50. A sealing ring 56 is provided on the inner wall of the valve stem hole 55. The sealing ring 56 contacts the valve stem 612. A baffle 613 is provided on the valve stem 612. A closing valve spring 62 is sleeved on the outside of the valve stem 612. The two ends of the closing valve spring 62 abut against the limiting frame 54 and the baffle 613 respectively. The closing valve spring 62 acts on the valve core 61 through the baffle 613 and the valve stem 612 and is used to push the valve core 61 to close.

[0055] The shaking motor 24 is connected to the valve core 61 via a linkage assembly and is used to drive the valve core 61 to open intermittently. The specific structure of the linkage assembly and the specific connection relationship between the shaking motor 24, the linkage assembly, and the valve core 61 are as follows: The linkage assembly includes a lever 63, a roller 64, a rack 65, an incomplete gear 66, and a return spring 67. A bracket 57 is provided on the semen storage cylinder 50. The lever 63 is hinged to the bracket 57 on the semen storage cylinder 50 via a pivot 631. A sliding groove 632 is provided on the lever 63. A pin 614 is provided on the valve stem 612. The pin 614 passes through the sliding groove 632. The roller 64 is rotatably located at the end of the lever 63. A guide sleeve 58 is provided on the semen storage cylinder 50. The rack 65 is slidably located on the guide sleeve 58 of the semen storage cylinder 50. The incomplete gear 66 is rotatably located on the guide sleeve 58 of the semen storage cylinder 50.

[0056] The shaking motor 24 is connected to the incomplete gear 66 via the transmission assembly 68 and is used to drive the incomplete gear 66 to rotate. The incomplete gear 66 can intermittently mesh with the rack 65 to intermittently push the rack 65 to slide in the direction of contact with the roller 64. When the rack 65 contacts the roller 64, it pushes the lever 63 to rotate around the pivot 631. Then, it pushes the pin 614 through the inner wall of the sliding groove 632 of the lever 63, thereby driving the valve stem 612 and the valve core 61 to slide, opening the valve core 61 so that the fresh semen in the semen storage cylinder 50 is discharged through the channel 53, the valve chamber 52 and the semen discharge nozzle 51 and falls into the dilution cup 40. When the incomplete gear 66 separates from the rack 65, it pushes the valve core 61 to close under the elastic force of the valve closing spring 62. The transmission assembly 68 includes a secondary transmission gear 681, a drive shaft 682, and a driven shaft 683. The secondary transmission gear 681 is rotatably mounted on the frame 10. A main transmission gear 233, which meshes with the secondary transmission gear 681, is mounted on the rotating shaft 232. The drive shaft 682 is rotatably mounted on the frame 10 in a vertical direction. The drive shaft 682 is connected to the secondary transmission gear 681 via a first transmission shaft 684. More specifically, the first transmission shaft 684 is rotatably mounted on the frame 10, and its two ends are connected to the secondary transmission gear 681 and the drive shaft 682 via a first bevel gear pair 686 and a second bevel gear pair 687, respectively.

[0057] The drive shaft 682 has a square hole along its axial direction. The driven shaft 683 is rotatably mounted on the lifting seat 70 along its vertical direction. The driven shaft 683 has a square rod 6831 along its axial direction, which is slidably inserted into the square hole of the drive shaft 682. The driven shaft 683 is connected to the incomplete gear 66 through a second transmission shaft 685. More specifically, the second transmission shaft 685 is rotatably mounted on the lifting seat 70, and its two ends are connected to the driven shaft 683 and the incomplete gear 66 through a third bevel gear pair 688 and a fourth bevel gear pair 689, respectively.

[0058] The semen storage cylinder 50 is provided with a stop block 59, and the rack 65 is provided with a column 651. The column 651 slides through the stop block 59. The end of the column 651 away from the rack 65 is provided with a flange 652. The return spring 67 is sleeved on the outside of the column 651. The two ends of the return spring 67 abut against the stop block 59 and the flange 652 respectively. The return spring 67 acts on the rack 65 through the flange 652 and the column 651, and is used to push the rack 65 to slide in the direction of separation from the roller 64.

[0059] This application also discloses a method for preparing frozen diluted bovine semen.

[0060] A method for preparing frozen diluted boar semen, based on the aforementioned apparatus for preparing frozen diluted boar semen, includes the following steps:

[0061] S1. Pour the prepared diluent into the dilution cup 40, and heat the diluent in the dilution cup 40 through the first heater 30. At the same time, pour the collected fresh semen into the semen storage cylinder 50, and heat the fresh semen in the semen storage cylinder 50 through the second heater 80. Heat the diluent in the dilution cup 40 and the fresh semen in the semen storage cylinder 50 to the required temperature (this temperature is common knowledge in the field and can be set conventionally as needed). Then, drive the rotating shaft 232 and the rotary arm 23 to rotate through the shaking motor 24. The rotary arm 23 drives the shaking table 21 to shake, and drives the first heater 30 and the dilution cup 40 to shake.

[0062] While the rocking motor 24 drives the rotating shaft 232 to rotate, it drives the incomplete gear 66 to rotate through the main transmission gear 233, the secondary transmission gear 681, the first transmission shaft 684, the drive shaft 682, the driven shaft 683, and the second transmission shaft 685. This causes the incomplete gear 66 to intermittently mesh with the rack 65, intermittently pushing the rack 65 to slide in the direction of contact with the roller 64. When the rack 65 contacts the roller 64, it pushes the lever 63 to rotate around the pivot 631. Then, it pushes the pin 614 through the inner wall of the lever 63's slide groove 632, thereby causing the valve stem 612 and the valve core 61 to slide and open the valve core 61. When the incomplete gear 66 separates from the rack 65, it pushes the valve core 61 to close under the elastic force of the closing spring 62, thereby driving the valve core 61 to open intermittently.

[0063] When valve core 61 is opened, the fresh semen in the semen storage cylinder 50 is discharged through channel 53, valve chamber 52 and semen discharge nozzle 51. After being discharged through the semen discharge nozzle 51, the fresh semen in the semen storage cylinder 50 is poured into the diluent for mixing and dilution. Because valve core 61 is opened intermittently, the fresh semen in the semen storage cylinder 50 is poured into the diluent multiple times for dilution, thus giving the sperm time to adapt to the new environment. Through gradient concentration transition and uniform mixing, sperm stress damage is avoided from the aspects of osmotic pressure protection and component adaptation, ultimately ensuring the overall vitality and survival time of the diluted semen, providing a basis for subsequent preservation.

[0064] S2. After the fresh semen is diluted, rotate the screw 100 to drive the lifting seat 70, the second heater 80 and the semen storage cylinder 50 to rise. Then remove the clamping bolt 92, remove the pressure plate 91 from the vertical column 312, remove the dilution cup 40 from the first heater 30, seal the diluted semen, and then put it into a constant temperature chamber for equilibration.

[0065] S3. Fill the balanced semen into the plastic tube using a thin tube filling machine, and seal the tube immediately after filling.

[0066] S4. Place the sealed thin tube neatly into the programmable freezer, and set the freezing curve after placing it in the instrument.

[0067] S5. Randomly sample and thaw frozen semen for testing. If the test is qualified, transfer the frozen semen to a liquid nitrogen tank for storage.

[0068] Steps S2 to S5 in the above-mentioned method for preparing frozen diluted boar semen are existing technologies in the field, and their specific steps can be implemented by those skilled in the art, and will not be described in detail here.

[0069] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, all equivalent changes made to the structure, shape, and principle of this application should be covered within the scope of protection of this application.

Claims

1. An apparatus for preparing frozen and diluted bovine semen, characterized in that, include: Rack (10); The shaking mechanism (20) includes a shaking table (21), a swing frame (22), a rotary arm (23), and a shaking motor (24). The swing frame (22) is hinged to the frame (10) via a first hinge shaft (11). The shaking table (21) is hinged to the swing frame (22) via a second hinge shaft (211). The axis of the first hinge shaft (11) and the axis of the second hinge shaft (211) are perpendicular to each other. The bottom of the shaking table (21) is provided with a downwardly extending connecting rod (212). The shaking motor (24) is fixed on the frame (10). The rotary arm (23) is connected to the output shaft of the shaking motor (24). The rotary arm (23) is provided with a ball head sleeve (231). The connecting rod (212) is provided with a ball head (213). The ball head (213) is spherically hinged to the ball head sleeve (231). The first heater (30) is disposed on the shaking table (21); A dilution cup (40) is detachably connected to a first heater (30) for heating the dilution cup (40).

2. The apparatus for preparing frozen and diluted bovine semen according to claim 1, characterized in that, It also includes a semen storage cylinder (50), a valve assembly (60), a lifting seat (70), and a second heater (80). The lifting seat (70) is slidably mounted on the frame (10) in a vertical direction. The frame (10) is provided with a lifting mechanism for driving the lifting seat (70) to move vertically. The second heater (80) is mounted on the lifting seat (70). The semen storage cylinder (50) is connected to the second heater (80) and is located above the dilution cup (40). The second heater (80) is used to heat the semen storage cylinder (50). The bottom of the semen storage cylinder (50) is provided with a semen discharge nozzle (51). The valve assembly (60) is used to control the opening and closing of the semen discharge nozzle (51).

3. The apparatus for preparing frozen diluted boar semen according to claim 1, characterized in that, It also includes a clamping element (90). The first heater (30) includes a first housing (31), which is fixed on the shaking table (21). The first housing (31) has a cavity (311) for accommodating a dilution cup (40). The first housing (31) is filled with a heat-conducting liquid. The first housing (31) has a first temperature control unit for controlling the temperature of the heat-conducting liquid in the first housing (31). The dilution cup (40) is placed in the cavity (311). The top of the dilution cup (40) is provided with a structure that can connect with the first housing. The upper surface of the body (31) is abutted by a limiting plate (41). The clamping member (90) includes a pressure plate (91) and a clamping bolt (92). The first housing (31) is provided with a vertical column (312) and a screw hole (313). The pressure plate (91) is slidably sleeved on the vertical column (312). The pressure plate (91) is provided with a through hole (911). The clamping bolt (92) passes through the through hole (911) and is screwed to the first housing (31) through the screw hole (313). The pressure plate (91) is provided with a pad (93) for abutting against the limiting plate (41).

4. The apparatus for preparing frozen and diluted boar semen according to claim 2, characterized in that, The second heater (80) includes a second housing (81), which is fixed on the lifting seat (70). The semen storage cylinder (50) is fixedly inserted through the second housing (81) in a vertical direction. The second housing (81) is filled with heat-conducting liquid. A second temperature control unit is provided on the second housing (81) for temperature control of the heat-conducting liquid in the second housing (81).

5. The apparatus for preparing frozen and diluted bovine semen according to claim 2, characterized in that, The valve assembly (60) includes a valve core (61), a valve closing spring (62), and a linkage assembly. The semen storage cylinder (50) is provided with a valve cavity (52) communicating with the semen discharge nozzle (51). The semen storage cylinder (50) is also provided with a channel (53) communicating with the valve cavity (52) and the inside of the semen storage cylinder (50). The valve core (61) is located in the valve cavity (52) and is used to open and close the connection between the valve cavity (52) and the channel (53). The valve closing spring (62) acts on the valve core (61) and is used to push the valve core (61) to close. The rocking motor (24) is connected to the valve core (61) through the linkage assembly and is used to drive the valve core (61) to open intermittently.

6. The apparatus for preparing frozen and diluted bovine semen according to claim 5, characterized in that, The semen storage cylinder (50) is provided with a limiting frame (54), the valve core (61) is provided with a valve stem (612), the valve stem (612) slides through the semen storage cylinder (50) and the limiting frame (54), the valve stem (612) is provided with a baffle (613), the valve closing spring (62) is sleeved on the outside of the valve stem (612), and the two ends of the valve closing spring (62) abut against the limiting frame (54) and the baffle (613) respectively.

7. The apparatus for preparing frozen diluted bovine semen according to claim 6, characterized in that, The linkage assembly includes a lever (63), a roller (64), a rack (65), an incomplete gear (66), and a return spring (67). The lever (63) is hinged to the semen storage cylinder (50), and a groove (632) is provided on the lever (63). A pin (614) is provided on the valve stem (612), and the pin (614) passes through the groove (632). The roller (64) is rotatably mounted on the end of the lever (63), and the rack (65) is slidably mounted on the semen storage cylinder (50). The incomplete gear (66) is rotatably mounted on the semen storage cylinder (50). The shaking motor (24) is connected to the incomplete gear (66) through the transmission assembly (68) and is used to drive the incomplete gear (66) to rotate. The incomplete gear (66) can intermittently mesh with the rack (65) to intermittently push the rack (65) to slide in the direction of contact with the roller (64). The return spring (67) acts on the rack (65) and is used to push the rack (65) to slide in the direction of separation from the roller (64).

8. The apparatus for preparing frozen and diluted boar semen according to claim 7, characterized in that, The semen storage cylinder (50) is provided with a stop block (59), and the rack (65) is provided with a column (651). The column (651) slides through the stop block (59). The end of the column (651) away from the rack (65) is provided with a flange (652). The return spring (67) is sleeved on the outside of the column (651). The two ends of the return spring (67) abut against the stop block (59) and the flange (652) respectively.

9. The apparatus for preparing frozen and diluted boar semen according to claim 7, characterized in that, The transmission assembly (68) includes a secondary transmission gear (681), a drive shaft (682), and a driven shaft (683). The secondary transmission gear (681) is rotatably mounted on the frame (10). A rotating shaft (232) is provided on the rotary arm (23). The rotating shaft (232) is fixedly connected to the output shaft of the rocking motor (24). A primary transmission gear (233) meshing with the secondary transmission gear (681) is provided on the rotating shaft (232). The drive shaft (682) is rotatably mounted on the frame (10) in a vertical direction. On 10), the drive shaft (682) is connected to the secondary transmission gear (681) through the first transmission shaft (684). The drive shaft (682) has a square hole along the axial direction. The driven shaft (683) is rotatably mounted on the lifting seat (70) along the vertical direction. The driven shaft (683) has a square rod (6831) along the axial direction. The square rod (6831) is slidably inserted into the square hole of the drive shaft (682). The driven shaft (683) is connected to the incomplete gear (66) through the second transmission shaft (685).

10. A method for preparing frozen diluted bovine semen, characterized in that, The apparatus for preparing frozen diluted bovine semen according to claim 1 includes the following steps: S1. Pour the prepared diluent into the dilution cup (40), heat the diluent in the dilution cup (40) through the first heater (30), then drive the rotary arm (23) to rotate through the shaking motor (24), drive the shaking table (21) to shake through the rotary arm (23), and drive the first heater (30) and the dilution cup (40) to shake. At the same time, pour the collected fresh essence into the diluent for mixing and diluting the fresh essence. S2. Remove the dilution cup (40) from the first heater (30), seal the diluted semen, and then place it in a constant temperature chamber for equilibration. S3. Fill the balanced semen into plastic capillary tubes using a capillary tube filling machine, and seal the tubes immediately after filling using a sealing machine; S4. Place the sealed thin tube neatly into the programmable freezer, and set the freezing curve after placing it into the instrument; S5. Randomly sample and thaw frozen semen for testing. If the test is qualified, transfer the frozen semen to a liquid nitrogen tank for storage.

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