Mouse intestinal nerve plexus patch and preparation method thereof
By peeling muscle tissue along the direction of the circular muscle and fixing it with paraformaldehyde and treating it with Triton X-100 solution, the problems of time-consuming and technically demanding enteric plexus detachment were solved, enabling rapid acquisition of complete enteric plexus patches.
CN121896149APending Publication Date: 2026-04-21SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information
- Application Number
- CN202511840915.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-09
- Publication Date
- 2026-04-21
AI Technical Summary
Technical Problem
Existing methods for ripping the enteric nerve plexus are time-consuming and technically demanding, making it difficult to quickly obtain complete enteric nerve plexus tissue.
Method used
Muscle tissue was dissected along the direction of the circular muscle, and mouse enteric plexus patches were prepared by combining paraformaldehyde fixation and Triton X-100 solution treatment.
Benefits of technology
It enables rapid and convenient acquisition of more complete enteric nerve plexus tissue, reducing operation time and technical difficulty.
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Abstract
The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.
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