Preparation method and use of cell-derived nanovesicles

By improving the cell-derived nanovesicle preparation method, and combining low-speed centrifugation and protective extraction solution, the problems of easy damage and poor stability of vesicles in the existing technology have been solved, and efficient and stable nanovesicle preparation has been achieved for promoting tissue repair and skin care.

CN121896162BActive Publication Date: 2026-06-16江苏赛亿生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
江苏赛亿生物技术有限公司
Filing Date
2026-03-16
Publication Date
2026-06-16

AI Technical Summary

Technical Problem

Existing technologies for isolating and purifying extracellular vesicles (EVs) from complex biological systems suffer from low efficiency, limited scale, vesicle susceptibility to damage, and difficulty in balancing high yield and high stability. In particular, when obtaining specific subpopulations (such as 30-150nm EVs), traditional methods such as ultracentrifugation can lead to vesicle rupture, fusion, or leakage of contents.

Method used

A method for preparing cell-derived nanovesicles was adopted, which included physically disrupting umbilical cord mesenchymal stem cells and mixing them with PEG-6000, sodium chloride and protective additives. After standing, the mixture was centrifuged at low speed and combined with a PEG-sodium chloride precipitation system to avoid ultra-high speed centrifugation. Squalane and ceramide lipids were used to protect the vesicle membrane and construct a stable extraction system.

Benefits of technology

The obtained nanovesicles have uniform particle size, good membrane integrity, and high bioactivity. They can promote the proliferation and migration of human skin fibroblasts, significantly improve the yield and stability of vesicles, and are suitable for pharmaceutical compositions and skin care products. They have good skin biocompatibility and safety, and promote wound repair.

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Abstract

The application relates to the technical field of biology, in particular to a preparation method and application of cell-derived nanovesicles. The preparation method comprises the following steps: physically crushing umbilical cord mesenchymal stem cells to obtain a cell crushing solution; mixing the cell crushing solution with an extracting solution at a volume ratio of 3:1-6:1, and standing for 10-30 hours; centrifuging and collecting the precipitate to obtain the cell-derived nanovesicles. The extracting solution comprises PEG-6000, sodium chloride and a protective additive, and the solvent is a PBS buffer. The method can efficiently prepare nanovesicles with complete membrane structure and high biological activity under mild conditions by physical crushing and purification with the extracting solution containing the specific protective additive, and effectively overcomes the defects of traditional ultracentrifugation, such as small scale, low efficiency and easy damage to the vesicles. The application further provides the nanovesicles obtained by the method and the application of the nanovesicles in preparing a skin care product, a freeze-dried preparation and a pharmaceutical composition for promoting skin wound healing.
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