Anti-cheating pretreatment sampling bottle and sampling method

Abstract

The present application relates to the technical field of environmental monitoring, and discloses a kind of anti-cheating pretreatment sampling bottle and sampling method.The anti-cheating pretreatment sampling bottle includes bottle body, bottle cap, identity recognition module, reagent store, fluid driving mechanism, sensor module, optical detection module and control unit.The identity recognition module is used for operator authorization authentication;Sensor module real-time acquisition water quality parameter change data in mixing process;Optical detection module obtains the optical characteristic data of sample after mixing;Control unit records identity authentication data, water quality parameter change data and optical characteristic data, and generates the encrypted data block corresponding to the sample by encryption.This application builds an anti-cheating evidence chain of deep integration of electronic data and sample physical properties, gives the sample an unalterable digital identity at the moment of collection, solves the risk of fraud caused by the separation of electronic log and physical sample in traditional technology, and improves the anti-cheating ability of sampling link and the original representativeness of sample.

Description

Technical Field

[0001] This invention relates to the field of environmental monitoring technology, specifically to an anti-cheating pretreatment sampling bottle and sampling method. Background Technology

[0002] Water environment monitoring is the foundation of environmental protection and governance, and water sample collection is the starting point of the monitoring process. Its authenticity, representativeness and traceability directly determine the validity and value of subsequent analysis data.

[0003] Currently, widely used simple sampling bottles rely primarily on manual recording of sampling time and location, with handwritten labels affixed to the bottles as information carriers. This method has significant management loopholes; sampling information is easily tampered with or forged, labels are easily damaged and detached in humid environments, and it is difficult to effectively prevent cheating behaviors such as sample swapping and unauthorized addition of preservatives during sample transfer. This not only results in poor sample representativeness and weak anti-cheating capabilities but also makes information entry into the laboratory management system inefficient and prone to errors. To address this issue, existing technologies provide sampling bottles capable of recording real-time information such as water quality, pH, and conductivity, which improves the level of informatization in sampling to some extent. However, their anti-cheating function mainly relies on recording and verifying multiple water quality indicators one by one. The actual verification process is cumbersome and complex, involving purely electronic data recording and comparison. If encryption methods are breached or the electronic log is completely replaced, the risk of data falsification still exists. The encryption link between sample physical properties and electronic data is weak; even if the electronic log is accurate, it is still impossible to confirm that the sample in the sampling bottle is the original sample. Summary of the Invention

[0004] In view of this, the present invention provides an anti-cheating pretreatment sampling bottle and sampling method to solve the problems mentioned in the background art.

[0005] In a first aspect, the present invention provides an anti-cheating pretreatment sampling bottle, comprising:

[0006] The bottle body has a sample compartment suitable for holding water samples;

[0007] The bottle cap is detachably and sealingly attached to the bottle body;

[0008] An identity recognition module, installed on the bottle cap, is used to authorize and authenticate operators and generate identity authentication data;

[0009] At least one reagent compartment is disposed inside the bottle cap, the reagent compartment being used to store pretreatment reagents;

[0010] A fluid drive mechanism is disposed inside the bottle cap. The fluid drive mechanism includes a multi-way valve and a micro pump. The multi-way valve has multiple fluid ports for selective fluid connection to the reagent chamber, the inlet of the micro pump, and the water sample inlet, respectively. The outlet of the micro pump is fluidly connected to the sample chamber.

[0011] A sensor module is installed on the bottle body to collect real-time data on changes in water quality parameters during the injection of the pretreatment reagent.

[0012] An optical detection module is installed on the bottle body and is used to perform optical detection on the mixed sample in the bottle after the pretreatment reagent is injected to obtain optical feature data.

[0013] The control unit is disposed inside the bottle cap and is electrically connected to the identification module, the fluid drive mechanism, the sensor module and the optical detection module respectively;

[0014] The control unit is configured to record at least the identity authentication data, the water quality parameter change data, and the optical feature data, and to encrypt the recorded data using a preset encryption algorithm to generate an encrypted data block corresponding to the mixed sample in the bottle.

[0015] Beneficial Effects: By integrating an identity recognition module, a fluid drive mechanism, a sensor module, an optical detection module, and a control unit, a fraud-proof electronic evidence chain is formed, deeply integrated with the physical properties of the sample. The identity recognition module ensures the traceability of the sampling operation, preventing unauthorized personnel from operating it; the fluid drive mechanism enables automatic quantitative addition of pretreatment reagents, avoiding the dangers and inaccuracies of manual operation, and providing a standard basis for the subsequent generation of physical fingerprints; the sensor module captures real-time water quality parameter changes during the mixing process, recording the dynamic process of sample-reagent interaction; the optical detection module acquires the static optical characteristic data of the sample after mixing; the control unit encrypts the aforementioned identity authentication data, dynamic change data, and optical characteristic data to generate encrypted data blocks, achieving a strong binding between electronic data and the physical state of the sample. Through this design, each sample is given a unique digital identity corresponding to its own chemical composition, forming an immutable evidence chain from the moment of collection, thereby solving the fraud risk caused by the separation of electronic logs and physical samples in traditional technologies, improving the fraud prevention capabilities of the sampling process and the original representativeness of the samples.

[0016] In some embodiments, the control unit includes:

[0017] Main control unit;

[0018] The spatiotemporal information module is electrically connected to the main control unit and is used to acquire the location information of the sampling location and the time information of the sampling time.

[0019] The data encryption and storage module is electrically connected to the main control unit and is used to encrypt the identity authentication data, the water quality parameter change data, the optical feature data, and the sampling time and location information obtained by the spatiotemporal information module, and to store the generated encrypted data block.

[0020] Beneficial effects: By providing location information of the sampling site and time information of the sampling time through the spatiotemporal information module, and adding encrypted data blocks, each sample not only has a chemical fingerprint bound to its physical properties, but also possesses tamper-proof spatiotemporal coordinates, thus improving the integrity of the evidence chain. As objective data independent of the operator's subjective recording, the sampling time and location information can effectively prevent cheating behaviors such as falsifying sampling locations and tampering with times, providing crucial evidence for accurate traceability and effectively improving the credibility of sampling information.

[0021] In some embodiments, the control unit further includes a communication module electrically connected to the main control unit, which is used to wirelessly transmit the encrypted data block to an external receiving device.

[0022] Beneficial effects: The communication module enables real-time wireless transmission of encrypted data blocks; after sampling, key evidence data can be immediately uploaded to the cloud platform or laboratory management system, avoiding the risk of data tampering or loss during physical transfer. Users can monitor sampling progress and sample status in real time, enabling remote verification and early warning, improving the timeliness and transparency of sampling management. The wireless transmission function provides a convenient data retrieval channel for subsequent laboratory verification, simplifying the verification process and improving overall work efficiency.

[0023] In some embodiments, the bottle cap is provided with a main control circuit board compartment, and the control unit is installed in the main control circuit board compartment.

[0024] Beneficial effects: The main control circuit board compartment, as an independent reinforced chamber, effectively isolates external moisture, corrosive gases, and mechanical impacts, ensuring stable operation of the control unit at the water environment sampling site.

[0025] In some embodiments, there are two reagent chambers, and the multi-way valve is a four-way valve with four fluid ports, which are respectively connected to the water sample inlet, the two reagent chambers, and the inlet of the micro-pump.

[0026] Beneficial Effects: This solution, by setting up two reagent compartments and employing a four-way valve, enables flexible selection and automatic addition of various pretreatment reagents. Specifically, for samples with different water qualities, such as acidic or alkaline water, the control unit can determine the type of reagent to be added based on real-time feedback data from sensors, and complete the quantitative injection of the corresponding reagent through precise switching of the four-way valve. This design allows a single sampling bottle to adapt to complex and varied sampling scenarios, eliminating the need to prepare multiple dedicated bottles for different water qualities, improving the versatility and sampling efficiency of the equipment, and also avoiding the errors and safety risks caused by manual judgment and manual addition.

[0027] In some embodiments, the bottle cap is further provided with an exhaust port, which is connected to the sample chamber and is used to maintain the internal and external air pressure balance during the sampling process.

[0028] Beneficial effects: The exhaust port solves the problem of air pressure balance during sampling and reagent injection; when the fluid-driven mechanism injects samples or reagents into the bottle, the exhaust port can promptly discharge excess gas inside, preventing the seal failure or increased injection resistance caused by increased pressure inside the bottle; when the sample is transported or the ambient temperature changes, the exhaust port can maintain the balance of internal and external air pressure, avoiding bottle deformation or sample contamination.

[0029] In some embodiments, the bottle cap is further provided with an LED status indicator light, which is electrically connected to the control unit and is used to display at least one of the following: battery status, sampling status, or signal strength.

[0030] Beneficial effects: The LED status indicators provide intuitive feedback on equipment status to on-site operators. Operators do not need additional instruments or to open the bottle cap; they can quickly understand information such as equipment battery level, sampling progress, and signal strength through the indicator light color or flashing pattern, facilitating timely problem detection and appropriate measures.

[0031] In some embodiments, the control unit controls the fluid drive mechanism to inject the pretreatment reagent into the water sample in a non-steady-state dynamic mode, the non-steady-state dynamic mode including a pulsed flow mode, a stepped flow mode, or a combination of both.

[0032] Beneficial Effects: By employing an unsteady-state kinetic mode for injecting pretreatment reagents, a unique mixing fingerprint is generated for each sample. Pulsed flow, stepped flow, or combinations thereof make the mixing process between the reagent and the water sample highly specific and unreproducible. The fluid behavior at the moment of mixing is influenced by multiple factors, including the original sample composition, reagent properties, injection rate, and pulse frequency. The resulting dynamic process cannot be faked through post-process simulation or simple dilution. This design upgrades anti-cheating measures from static data comparison to dynamic process verification, effectively eliminating the possibility of falsification by replacing samples or adjusting reagent dosages, thus enhancing the sophistication and reliability of anti-cheating technology.

[0033] In some embodiments, the water quality parameter change data collected by the sensor module is: a dynamic change dataset formed during the mixing process of the unsteady dynamic mode; the control unit records the dynamic change dataset as a process fingerprint characterizing the current operation in the encrypted data block.

[0034] Beneficial Effects: By using the dynamic change dataset collected by the sensor module as a process fingerprint and recording it in an encrypted data block, accurate reproduction and verification of the entire sampling and mixing process are achieved. The dynamic change dataset can completely record the real-time changes in parameters such as pH and conductivity of the sample at the moment of reagent injection, containing reliable information such as mixing rate and reaction kinetics, and has extremely high uniqueness and information density. Although the laboratory does not need to reproduce this dynamic process during verification, it serves as an important component of the encrypted data block, forming a complete chain of evidence together with other data. Once the sample is switched or diluted, it will be impossible to compare subsequent physicochemical anchor values, thus ensuring multi-layered and multi-dimensional protection of the anti-cheating system.

[0035] Secondly, the present invention also provides a sampling method for anti-cheating pretreatment sampling bottles, comprising the following steps:

[0036] Authorized Startup: The operator's identity is authenticated through the identity recognition module. After successful authentication, the control unit is unlocked and enters the sampling state.

[0037] Sampling and pretreatment: Water samples are collected and placed into the bottle, and the pretreatment reagent is injected into the water sample in a preset mode through a fluid drive mechanism. The water quality parameter change data during the mixing process is collected in real time through the sensor module to generate a process fingerprint.

[0038] In-situ detection: After the pretreatment reagent is injected, the mixed sample in the bottle is optically detected by the optical detection module to obtain optical feature data as physicochemical anchor point values;

[0039] Data locking: The control unit encrypts the process fingerprint, the physicochemical anchor value, and the sampling information, generates an encrypted data block, and stores it; the sampling information includes identity authentication data generated by the identity recognition module, sampling time and location information, and volume and concentration data of the sample and pretreatment reagent.

[0040] Beneficial Effects: This sampling method constructs a complete anti-cheating process from sampling to data generation through four steps: authorized initiation, sampling and pretreatment, in-situ detection, and data locking. Authorized initiation ensures that only certified personnel can operate the equipment; the sampling and pretreatment step automatically adds a protective agent while generating an unreproducible process fingerprint; the in-situ detection step transforms the final physical state of the sample into verifiable physicochemical anchor values; and the data locking step encrypts and stores all key information, forming a unique digital identity corresponding to the sample. This sampling method deeply integrates physical samples with electronic evidence, giving each sample an immutable electronic certificate from the moment of collection. Laboratories only need to retest the physicochemical anchor values ​​to quickly verify the authenticity and integrity of the sample, effectively preventing cheating throughout the entire sampling process, while simplifying the verification process and improving the overall credibility and efficiency of water environment monitoring. Attached Figure Description

[0041] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0042] Figure 1 This is an external schematic diagram of the anti-cheating pretreatment sampling bottle according to an embodiment of the present invention;

[0043] Figure 2 This is a schematic diagram of the anti-cheating pretreatment sampling bottle according to an embodiment of the present invention;

[0044] Figure 3 This is a schematic diagram illustrating the working principle of the anti-cheating pretreatment sampling bottle according to an embodiment of the present invention.

[0045] Explanation of reference numerals in the attached figures:

[0046] 1. Identification module; 2. LED status indicator; 3. Exhaust port; 4. Bottle body; 5. Bottle cap; 6. Main control circuit board compartment; 7. Reagent compartment A; 8. Reagent compartment B; 9. Multi-way valve; 10. Micro pump; 11. Sensor module; 12. Optical detection module. Detailed Implementation

[0047] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0048] The following is combined with Figures 1 to 3 The following describes embodiments of the present invention.

[0049] According to an embodiment of the present invention, in one aspect, an anti-cheating pretreatment sampling bottle is provided, see [link to previous article]. Figures 1 to 3 The anti-cheating pretreatment sampling bottle includes a bottle body 4, a cap 5, an identification module 1, a reagent compartment, a fluid drive mechanism, a sensor module 11, an optical detection module 12, and a control unit. The bottle body 4 has a sample compartment suitable for holding water samples. The bottle body 4 and cap 5 can be modularly detachable, with the cap 5 detachably sealed to the bottle body 4, which is a single-use component. The reagent compartment is located inside the cap 5 and is used to store pretreatment reagents. The fluid drive mechanism and control unit are located inside the cap 5 and are isolated from each other. The control unit is electrically connected to the identification module 1, the fluid drive mechanism, the sensor module 11, and the optical detection module 12, respectively. The identification module 1 is located on the cap 5 and is used to authorize and authenticate operators and generate identification authentication data.

[0050] In one specific embodiment, the identity recognition module 1 includes a fingerprint reader or an RFID reader. The fingerprint reader is used to collect the operator's fingerprint and compare it with a pre-stored fingerprint template. The RFID reader is used to read the RFID identity card held by the operator, thereby ensuring at the physical level that only authorized personnel can initiate the sampling process.

[0051] like Figure 2 As shown, the fluid drive mechanism includes a multi-way valve 9 and a micro pump 10. The multi-way valve 9 has multiple fluid ports to selectively fluidly connect to the reagent chamber, the inlet of the micro pump 10, and the water sample inlet, respectively. The outlet of the micro pump 10 is fluidly connected to the sample chamber.

[0052] The sensor module 11 is installed on the bottle body 4. The sensor module 11 is used to collect data on the changes in water quality parameters during the injection of pretreatment reagents.

[0053] In one specific embodiment, the sensor module 11 includes a pH sensor, a conductivity sensor, and a temperature sensor, which can be integrated on the same probe or set separately in the sample chamber to continuously monitor water quality changes during the mixing process. The sampling frequency is set by the control unit.

[0054] An optical detection module 12 is mounted on the bottle body 4. This module is used to perform optical detection on the mixed sample inside the bottle after the pretreatment reagent is injected, acquiring optical characteristic data. The control unit records the absorbance ratio and other optical characteristic data as optical characteristic data within an encrypted data block.

[0055] In one specific embodiment, the optical detection module 12 is configured as a miniature dual-wavelength LED photometer, which has two built-in LED light sources and photodetectors of different wavelengths to quickly measure the absorbance of the sample at a specific wavelength and calculate the absorbance ratio.

[0056] In this embodiment, the control unit is configured to: record at least identity authentication data, water quality parameter change data, and optical feature data, and encrypt the recorded data using a preset encryption algorithm to generate an encrypted data block corresponding to the mixed sample inside the bottle 4.

[0057] In a specific embodiment, the control unit includes a main control unit, a spatiotemporal information module, and a data encryption and storage module; the spatiotemporal information module is electrically connected to the main control unit and is used to acquire the location information of the sampling location and the time information of the sampling time; the data encryption and storage module is electrically connected to the main control unit and is used to encrypt the identity authentication data, water quality parameter change data, optical feature data, and the sampling time and location information acquired by the spatiotemporal information module, and store the generated encrypted data block.

[0058] In this scheme, the spatiotemporal information module provides the location information of the sampling site and the time information of the sampling time. By incorporating encrypted data blocks, each sample not only possesses a chemical fingerprint bound to its physical properties but also has immutable spatiotemporal coordinates, thus enhancing the integrity of the evidence chain. As objective data independent of the operator's subjective recording, the sampling time and location information effectively prevents fraudulent activities such as falsifying sampling locations and tampering with times, providing crucial evidence for accurate traceability and significantly improving the credibility of the sampling information.

[0059] In a further embodiment, the control unit also includes a communication module electrically connected to the main control unit. The communication module is used to wirelessly transmit encrypted data blocks to an external receiving device. This enables real-time wireless transmission of encrypted data blocks. After sampling, key evidence data can be immediately uploaded to a cloud platform or laboratory management system, avoiding the risk of data tampering or loss during physical transfer. Users can monitor sampling progress and sample status in real time, enabling remote verification and early warning, thus improving the timeliness and transparency of sampling management. The wireless transmission function provides a convenient data retrieval channel for subsequent laboratory verification, simplifying the verification process and improving overall work efficiency.

[0060] In the specific implementation selection, the core of the control unit is the main control unit, which can be a low-power microcontroller or embedded processor with a built-in real-time operating system. It is responsible for coordinating the work of each module, executing decision-making logic, and data encryption. The identification module 1 can use an RFID chip or an NFC chip to store the unique ID of the sampling bottle. The spatiotemporal information module includes a GPS positioning unit or a BeiDou positioning unit for obtaining precise geographical location and a network clock unit for obtaining precise time. The communication module is at least one of a 4G communication unit, a 5G communication unit, an NB-IoT communication unit, or a Bluetooth communication unit.

[0061] In a specific embodiment, the micropump 10 is configured as a plunger pump or a peristaltic pump, which is used to accurately measure and record the flow rate and volume data of the water sample and pretreatment reagent flowing through the micropump 10.

[0062] In specific structural embodiments, such as Figure 1 As shown, the bottle cap 5 is also equipped with an exhaust port 3, which is connected to the sample chamber. The exhaust port 3 is used to maintain the internal and external air pressure balance during the sampling process. The exhaust port 3 solves the air pressure balance problem during sampling and reagent injection. When the fluid drive mechanism injects the sample or reagent into the bottle 4, the exhaust port 3 can promptly discharge excess gas inside, preventing the seal failure or increased injection resistance caused by the pressure rise inside the bottle. When the sample is transported or the ambient temperature changes, the exhaust port 3 can maintain the internal and external air pressure balance, avoiding deformation of the bottle 4 or sample contamination.

[0063] In specific embodiments, such as Figure 1 As shown, the bottle cap 5 is also equipped with an LED status indicator 2, which is electrically connected to the control unit and is used to display at least one of the following: battery level, sampling status, or signal strength. The LED status indicator 2 provides intuitive equipment status feedback to on-site operators. Operators do not need additional instruments or to open the bottle cap 5; they can quickly understand information such as battery level, sampling progress, and signal strength through the indicator light's color or flashing pattern, facilitating timely problem detection and appropriate action.

[0064] In a specific embodiment, the control unit may be configured with a human-machine interaction module, such as a fingerprint reader or an RFID reader, for authorizing the opening of the anti-fraud pretreatment sampling bottle.

[0065] In a specific embodiment, the bottle cap 5 contains a main control circuit board compartment 6, and the control unit is installed inside the main control circuit board compartment 6. The main control circuit board compartment 6 serves as an independent reinforced chamber, effectively isolating external moisture, corrosive gases, and mechanical impacts, ensuring the stable operation of the control unit at the water environment sampling site.

[0066] In a specific embodiment, two reagent compartments are provided, and the multi-way valve 9 is configured as a four-way valve, such as... Figure 2 As shown, the multi-port valve 9 has four fluid ports, which are connected to the water sample inlet, two reagent chambers, and the inlet of the micro-pump 10, respectively. In the specific implementation, the two reagent chambers are designated as reagent chamber A7 and reagent chamber B8, which are respectively loaded with acidic and alkaline protective agents.

[0067] This solution, by setting up two reagent compartments and employing a four-way valve, enables flexible selection and automatic addition of various pretreatment reagents. Specifically, for samples with different water qualities, such as acidic or alkaline water, the control unit can determine the type of reagent to be added based on real-time feedback data from sensors, and complete the quantitative injection of the corresponding reagent through precise switching of the four-way valve. This design allows a single sampling bottle to adapt to complex and varied sampling scenarios, eliminating the need to prepare multiple dedicated bottles for different water qualities, improving the versatility of the equipment and sampling efficiency, while also avoiding the errors and safety risks associated with manual judgment and manual addition.

[0068] For specific sampling and preprocessing operations, such as Figure 3 As shown:

[0069] 1. Quantitative Sample Aspiration Section: The control unit instructs multi-port valve 9 to connect the water sample inlet and the common outlet. The high-precision pump is activated to accurately extract a water sample of volume V1. During the water sample collection process, the control unit continuously reads the pH value and conductivity of the water sample solution.

[0070] 2. Quantitative Reagent Aspiration Section: The control unit instructs multi-port valve 9 to switch between reagent compartments A7 and B8. The control unit has pre-set decision logic:

[0071] If pH > 9, the water is considered alkaline and requires acidification and fixation. Select reagent compartment A7 (acidic).

[0072] If pH < 6, the water is considered acidic and requires alkalization to fix specific pollutants. Select reagent compartment B8 (alkaline).

[0073] If the conductivity value spikes sharply, it may indicate high-salinity wastewater or a specific pollution event, triggering a special warning and allowing the selection of specific reagents according to the contingency plan.

[0074] The control unit implements an adaptive decision-making process, which includes at least sensor readings, decision-making procedures, the final selected reagent, and the amount of reagent. The decision-making process can be recorded and encrypted.

[0075] 3. Propulsion and Mixing: The control unit instructs the multi-way valve 9 to switch again, connecting the reagent chamber and the sample chamber. The micro-pump 10 starts, propelling the reagent into the sample chamber at the set flow rate to achieve mixing.

[0076] Of course, the reagent chamber can be loaded with other reagents, such as fixatives; during operation, the control unit changes the flow path of the multi-way valve 9 and records the flow data of the reagent chamber and the water sample.

[0077] In a specific embodiment, the control unit controls the fluid drive mechanism to inject pretreatment reagents into the water sample in a non-steady-state dynamic mode. This non-steady-state dynamic mode includes pulsed flow, stepped flow, or a combination of both. By employing a non-steady-state dynamic mode to inject the pretreatment reagents, a unique mixing fingerprint is generated for each sample. The pulsed flow, stepped flow, or their combination makes the mixing process between the reagent and the water sample highly specific and unreproducible. The fluid behavior at the moment of mixing is influenced by multiple factors, including the original composition of the sample, the properties of the reagents, the injection rate, and the pulse frequency. The resulting dynamic process cannot be faked through post-processing simulation or simple dilution. This design upgrades anti-cheating design from static data comparison to dynamic process verification, effectively eliminating the possibility of falsification by replacing samples or adjusting reagent dosages, thus improving the sophistication and reliability of the anti-cheating technology.

[0078] The anti-cheating pretreatment sampling bottle provided in this embodiment has the following working process and anti-cheating procedure:

[0079] Sampling personnel authenticate their identity through the identity recognition module 1; after successful authentication, the bottle cap 5 is unlocked, and the device enters the sampling state.

[0080] Connect the sampling tubing to begin sampling. The control unit automatically records data such as sample pH, conductivity, and volume. After sampling, cap 5 seals bottle 4, and the control unit adjusts the direction of multi-way valve 9 to automatically release the solvent from the reagent compartment. The main control unit records the reagent release status.

[0081] When bottle cap 5 is opened or closed for the first time, the main control unit automatically executes the following steps: It obtains the timestamp (T) and location coordinates (P) from the spatiotemporal information module, combines them with the unique ID of the sampling bottle and the operation log (L), such as: successful authentication, bottle cap 5 opened, bottle cap 5 closed, reagent released, and uses an encryption algorithm to generate a unique digital signature (S) for this data. The {ID,T,P,L,S} data is packaged into an encrypted data block, stored in the local chip, and can optionally be uploaded to the cloud monitoring platform via the communication module.

[0082] Upon receiving the samples, the laboratory or regulatory authority uses a reader to scan the sampling bottle and read the encrypted data block. The system verifies that the digital signature S is generated from the correct ID, T, P, and L. Any tampering with the time, location, or operation logs will cause verification to fail, thus immediately identifying cheating.

[0083] In a specific embodiment, the water quality parameter change data collected by the sensor module 11 is: a dynamic change dataset formed during the mixing process of the unsteady dynamic mode; the control unit records the dynamic change dataset as a process fingerprint characterizing this operation in the encrypted data block.

[0084] By using the dynamic change dataset collected by sensor module 11 as a process fingerprint and recording it in an encrypted data block, the precise reproduction and verification of the entire sampling and mixing process is achieved. The dynamic change dataset can completely record the real-time changes in parameters such as pH and conductivity of the sample at the moment of reagent injection, containing reliable information such as mixing rate and reaction kinetics, and possesses extremely high uniqueness and information density. Although the laboratory does not need to reproduce this dynamic process during verification, it serves as an important component of the encrypted data block, forming a complete chain of evidence along with other data. If the sample is switched or diluted, it will be impossible to compare subsequent physicochemical anchor values, thus ensuring multi-layered and multi-dimensional protection of the anti-cheating system.

[0085] The anti-cheating pretreatment sampling bottle provided in this embodiment integrates an identity recognition module 1, a fluid drive mechanism, a sensor module 11, an optical detection module 12, and a control unit to form an anti-cheating electronic evidence chain deeply integrated with the physical properties of the sample. The identity recognition module 1 ensures the traceability of the sampling operation, preventing unauthorized personnel from operating it; the fluid drive mechanism enables automatic quantitative addition of pretreatment reagents, avoiding the dangers and inaccuracies of manual operation and providing a standard basis for subsequent physical fingerprint generation; the sensor module 11 captures real-time water quality parameter changes during the mixing process, recording the dynamic process of sample-reagent interaction; the optical detection module 12 acquires the static optical characteristic data of the sample after mixing; the control unit encrypts the aforementioned identity authentication data, dynamic change data, and optical characteristic data to generate encrypted data blocks, achieving a strong binding between electronic data and the physical state of the sample. Through this design, each sample is given a unique digital identity corresponding to its own chemical composition, forming an unalterable evidence chain from the moment of collection. This solves the risk of forgery caused by the separation of electronic logs and physical samples in traditional technologies, improving the anti-cheating capability of the sampling process and the original representativeness of the sample.

[0086] The anti-cheating pretreatment sampling bottle provided in this embodiment achieves deep binding between electronic data and sample physical properties by constructing a multi-dimensional technical closed loop of unique ID, spatiotemporal lock, operation log, digital signature, and physicochemical characteristics. This forms an immutable, end-to-end evidence, fundamentally eliminating cheating in the sampling process. The fluid-driven mechanism enables automatic, quantitative, and sealed addition of pretreatment reagents, avoiding direct contact between operators and hazardous chemicals, simplifying on-site operations, and improving operational safety. Sampling information can be automatically collected and digitally managed throughout the entire process, seamlessly integrating with laboratory management systems to improve work efficiency while avoiding errors and omissions caused by manual recording.

[0087] The anti-cheating pretreatment sampling bottle provided in this embodiment has the following anti-cheating features:

[0088] 1. Start-up and baseline self-check: Before sampling, the system performs a self-check, records the initial volumes of reagent compartment A7 and reagent compartment B8, and stores them in the encrypted log as baseline data. This step provides a starting point for subsequent verification.

[0089] 2. Sampling and Process Fingerprint Generation: During precise mixing of samples and reagents, the system not only records volumes V1 and V2, but also controls and records a unique hybrid dynamics parameter, such as pulsed injection or stepwise mixing. Pulsed injection uses pulses of specific frequency and amplitude to drive the mixing, while stepwise mixing involves multiple, gradual mixing steps at different proportions. The resulting instantaneous data changes that can be captured by sensors are recorded, such as the dynamic change curve of the pH sensor reading at the moment of mixing, or the transition trajectory of conductivity. This dynamic change dataset is completely recorded as the process fingerprint of this operation.

[0090] 3. Creating a State Anchor Point: After mixing, the system immediately performs a rapid in-situ analysis of the mixture within the sample chamber. The built-in optical detection module 12 measures the absorbance ratio (R=A) of the mixed sample at a specific wavelength. 450 / A 650 This absorbance ratio is directly determined by the original sample concentration, the volume / concentration of the added reagent, and the hybrid dynamics. This ratio is used as a state anchor point and is encrypted and stored together with the aforementioned process fingerprint and operation log.

[0091] 4. Enhanced Anti-Fraud Verification: Traditional verification in laboratories involves checking electronic logs after receiving samples. This invention, however, incorporates unforgeable physicochemical steps:

[0092] Retesting state anchor point: The laboratory uses standard instruments to measure the absorbance ratio (R0) of the same sample at the same wavelength according to the procedure. V =A 450 / A 650The values ​​measured in the laboratory are compared with the state anchor point values ​​recorded on the sampling bottles.

[0093] If both are consistent within the error range, then it is triple confirmation:

[0094] ① The electronic logs were not tampered with;

[0095] ②The recorded volume ratio (V1 / V2) is accurate;

[0096] ③ The sample was not diluted or contaminated after sampling. If this is inconsistent, it proves that at least one link in the chain has been falsified.

[0097] In an exemplary embodiment, the preset encryption algorithm is as follows:

[0098] I. Data Collection. Collect the identification data of the sampling personnel, water quality parameters such as pH, conductivity, and temperature from the start to the end of sampling, and the dual-wavelength absorbance ratio R=A. 450 / A 650 .

[0099] 2. Generate the operation log string. Generate the string {L} from the above data according to a fixed format.

[0100] III. Calculate the hash value. The national standard SM3 hash algorithm is used to calculate the hash value of the string L, resulting in a fixed-length hash value S = SM3(L). The SM3 algorithm outputs 256 bits of binary data, which is usually represented as a 64-bit hexadecimal string.

[0101] IV. Generate encrypted data blocks. Generate a data block {D} from the device ID, sampling time, sampling location, physicochemical anchor point R, and data hash S, store it in the local chip, and simultaneously upload it to the cloud monitoring platform.

[0102] V. Laboratory Validation. Upon sample delivery, the validation personnel shall perform the following operations:

[0103] (1) Read and write the data block D stored in the sampling bottle to obtain the physicochemical anchor point R and the data hash S.

[0104] (2) Measure the current dual-wavelength absorbance ratio R of the sample using a standard spectrophotometer. V .

[0105] (3) If |R V If -R|>0.1, the sample state is determined to have changed, and the data is invalid; if |R|>0.1, the sample state is determined to have changed, and the data is invalid. V If -R|≤0.1, proceed to the next step.

[0106] (4) Extract information such as device ID, sampling time, and sampling location from the data block, and combine it with the identity information of the verification personnel to regenerate the string {L} in a fixed format. V}

[0107] (5) Calculate S using the same algorithm V =SM3(L V ).

[0108] (6) Compare H V If the value matches H in the data block, the data is considered complete and tamper-proof, and the sample is valid; otherwise, the data is considered tamper-proof, and the sample is invalid.

[0109] The reason for choosing 450nm and 650nm as detection wavelengths is that the pretreatment reagent will produce characteristic absorption at 450nm after reacting with common pollutants in the water. 650nm is used as a reference wavelength to eliminate interference from factors such as sample turbidity. Calculating the ratio of the two can effectively characterize the concentration of the target pollutant and improve detection stability.

[0110] According to an embodiment of the present invention, in another aspect, a sampling method for anti-cheating pretreatment sampling bottles is also provided, comprising the following steps:

[0111] Authorized start: The operator's identity is authenticated through the identity recognition module 1. After successful authentication, the control unit is unlocked and enters the sampling state.

[0112] Sampling and pretreatment: Water samples are collected and put into bottle 4, and pretreatment reagents are injected into the water samples in a preset mode through a fluid drive mechanism. The water quality parameter change data during the mixing process are collected in real time through sensor module 11 to generate process fingerprint;

[0113] In-situ detection: After the pretreatment reagent is injected, the mixed sample in the bottle is optically detected by the optical detection module 12 to obtain optical feature data as physicochemical anchor point value;

[0114] Data locking: The control unit encrypts the process fingerprint, physicochemical anchor value and sampling information, generates encrypted data blocks and stores them; the sampling information includes the identity authentication data generated by the identity recognition module 1, the sampling time and location information, and the volume and concentration data of the sample and pretreatment reagents.

[0115] This sampling method constructs a complete anti-cheating process from sampling to data generation through four steps: authorized initiation, sampling and pretreatment, in-situ detection, and data locking. Authorized initiation ensures that only certified personnel can operate the equipment; the sampling and pretreatment step automatically adds a protective agent while generating an unreproducible process fingerprint; the in-situ detection step transforms the final physical state of the sample into verifiable physicochemical anchor values; and the data locking step encrypts and stores all key information, forming a unique digital identity corresponding to the sample. This sampling method deeply integrates physical samples with electronic evidence, giving each sample an immutable electronic certificate from the moment of collection. Laboratories can quickly verify the authenticity and integrity of the sample simply by retesting the physicochemical anchor values, effectively preventing cheating throughout the entire sampling process, while simplifying the verification process and improving the overall credibility and efficiency of water environment monitoring.

[0116] Although embodiments of the invention have been described in conjunction with the accompanying drawings, those skilled in the art can make various modifications and variations without departing from the spirit and scope of the invention, and such modifications and variations all fall within the scope defined by the appended claims.

Claims

1. A cheat-proof pretreatment sampling bottle, characterized in that, include: The bottle body (4) has a sample compartment suitable for holding water samples; The bottle cap (5) is detachably and sealingly disposed on the bottle body (4); An identity recognition module (1) is installed on the bottle cap (5) to authorize and authenticate the operator and generate identity authentication data; At least one reagent compartment is disposed inside the bottle cap (5), the reagent compartment being used to store pretreatment reagents; A fluid drive mechanism is provided inside the bottle cap (5). The fluid drive mechanism includes a multi-way valve (9) and a micro pump (10). The multi-way valve (9) has multiple fluid ports to selectively fluidly connect to the reagent chamber, the inlet end of the micro pump (10), and the water sample inlet, respectively. The outlet end of the micro pump (10) is fluidly connected to the sample chamber. A sensor module (11) is installed on the bottle body (4) for real-time acquisition of water quality parameter changes during the injection of the pretreatment reagent; An optical detection module (12) is installed on the bottle body (4) and is used to perform optical detection on the mixed sample in the bottle after the pretreatment reagent is injected, and to obtain optical feature data. The control unit is located inside the bottle cap (5) and is electrically connected to the identity recognition module (1), the fluid drive mechanism, the sensor module (11) and the optical detection module (12), respectively. The control unit is configured to: record at least the identity authentication data, the water quality parameter change data and the optical feature data, and encrypt the recorded data using a preset encryption algorithm to generate an encrypted data block corresponding to the mixed sample in the bottle (4); The control unit controls the fluid drive mechanism to inject the pretreatment reagent into the water sample in an unsteady dynamic mode, the unsteady dynamic mode including pulse flow mode, step flow mode or a combination of the two; The water quality parameter change data collected by the sensor module (11) is: a dynamic change dataset formed during the mixing process of the unsteady dynamic mode; the control unit records the dynamic change dataset as a process fingerprint characterizing this operation in the encrypted data block.

2. The anti-cheating pretreatment sampling bottle according to claim 1, characterized in that, The control unit includes: Main control unit; The spatiotemporal information module is electrically connected to the main control unit and is used to acquire the location information of the sampling location and the time information of the sampling time. The data encryption and storage module is electrically connected to the main control unit and is used to encrypt the identity authentication data, the water quality parameter change data, the optical feature data, and the sampling time and location information obtained by the spatiotemporal information module, and to store the generated encrypted data block.

3. The anti-cheating pretreatment sampling bottle according to claim 2, characterized in that, The control unit also includes a communication module, which is electrically connected to the main control unit and is used to wirelessly transmit the encrypted data block to an external receiving device.

4. The anti-cheating pretreatment sampling bottle according to claim 1, characterized in that, The bottle cap (5) is provided with a main control circuit board compartment (6), and the control unit is installed in the main control circuit board compartment (6).

5. The anti-cheating pretreatment sampling bottle according to claim 1, characterized in that, The reagent compartment is provided in two places, and the multi-way valve (9) is set as a four-way valve. The multi-way valve (9) has four fluid ports, which are respectively connected to the water sample inlet, the two reagent compartments and the inlet of the micro pump (10).

6. The anti-cheating pretreatment sampling bottle according to claim 1, characterized in that, The bottle cap (5) is also provided with an exhaust port (3), which is connected to the sample chamber. The exhaust port (3) is used to maintain the internal and external air pressure balance during the sampling process.

7. The anti-cheating pretreatment sampling bottle according to claim 1, characterized in that, The bottle cap (5) is also provided with an LED status indicator (2), which is electrically connected to the control unit and is used to display at least one of the following: power status, sampling status, or signal strength.

8. A sampling method using anti-cheating pretreatment sampling bottles as described in any one of claims 1 to 7, characterized in that, Includes the following steps: Authorized start: The operator's identity is authenticated through the identity recognition module (1). After successful authentication, the control unit is unlocked and enters the sampling state. Sampling and pretreatment: Water samples are collected and put into the bottle (4), and the pretreatment reagent is injected into the water sample in a preset mode through the fluid drive mechanism. The water quality parameter change data during the mixing process is collected in real time through the sensor module (11) to generate process fingerprint; In-situ detection: After the pretreatment reagent is injected, the mixed sample in the bottle is optically detected by the optical detection module (12) to obtain optical feature data as physicochemical anchor point value; Data locking: The control unit encrypts the process fingerprint, the physicochemical anchor value and the sampling information, generates an encrypted data block and stores it; the sampling information includes the identity authentication data generated by the identity recognition module (1), the sampling time and location information, and the volume and concentration data of the sample and pretreatment reagent.

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