Pollen pini-lotus leaf composition based on morchella fermentation as well as preparation method and application of pollen pini-lotus leaf composition

Abstract

The invention belongs to the technical field of food processing and biological fermentation, and particularly relates to a toadstool fermentation-based pine pollen-lotus leaf composition as well as a preparation method and application thereof, and the composition is prepared from a compound substrate of toadstool fermentation pine pollen and lotus leaf. The pine pollen and the lotus leaves are fermented by the morchella esculenta, so that the obtained composition has remarkable effects of losing weight and reducing blood fat.

Description

Technical Field

[0001] This invention relates to a pine pollen-lotus leaf composition based on morel fermentation, its preparation method and application, belonging to the field of food processing and bio-fermentation technology. Background Technology

[0002] Obesity has become a global public health problem. A positive energy balance caused by a high-fat diet can lead to abnormal accumulation of white fat, often accompanied by a series of metabolic problems such as dyslipidemia, hepatic steatosis, and low-grade inflammation.

[0003] In the field of natural product development, pine pollen and lotus leaves are two promising medicinal and edible raw materials. Pine pollen is rich in sterols, polysaccharides, and flavonoids. Studies have shown that pine pollen and its extracts can lower serum total cholesterol (TC) and triglycerides (TG). Lotus leaves are the dried leaves of the lotus plant (Nelumbo nucifera), which contain lotus leaf alkaloids, flavonoids, and polysaccharides. Studies have shown that their active ingredients have lipid-lowering, antioxidant, and fatty acid synthase-inhibiting functions, and can promote fatty acid β-oxidation and reduce liver fat deposition. However, in traditional applications, pine pollen and lotus leaves are often consumed by directly brewing or grinding them. This simple physical processing method is difficult to effectively release and transform their active ingredients, limiting their further application and efficacy.

[0004] Microbial fermentation technology is considered a potential means to improve the flavor and efficacy of medicinal and edible raw materials. This method can enhance the bioactivity of raw materials by disrupting plant cell walls, transforming glycosides, and increasing the content of total phenols and flavonoids. However, currently, there are no mature technical solutions reported on how to specifically apply microbial fermentation technology to the combination of pine pollen and lotus leaves. Summary of the Invention

[0005] In view of the above-mentioned technical problems in the prior art, the present invention provides a pine pollen-lotus leaf composition based on morel fermentation, its preparation method and application.

[0006] The technical solution of the present invention to solve the above-mentioned technical problems is as follows: One of the objectives of this invention is to provide a pine pollen-lotus leaf composition based on morel fermentation, which is prepared by fermenting a compound substrate of pine pollen and lotus leaf using morel fermentation, wherein the weight ratio of pine pollen to lotus leaf is 3 to 6:1.

[0007] The beneficial effects of the above-mentioned technical solution of the present invention are as follows: the present invention can effectively destroy the cell wall structure of pine pollen and lotus leaf after fermentation with morel mushrooms, promote the release and transformation of active ingredients such as flavonoids, improve the dissolution rate and bioavailability of active ingredients, so that the composition can be better absorbed and utilized after entering the human body, thereby enhancing its physiological activities such as lowering blood lipids and promoting fat metabolism.

[0008] Furthermore, the composition is a dry powder with a moisture content of ≤8%.

[0009] Furthermore, the weight ratio of pine pollen to lotus leaf is 5:1.

[0010] Furthermore, the total phenol content of the composition is ≥5.5 mg gallic acid equivalent (GAE) / g dry product, and the total flavonoid content is ≥4.5 mg rutin equivalent (RE) / g dry product.

[0011] The second objective of this invention is to provide a method for preparing the pine pollen-lotus leaf composition based on morel fermentation as described above, comprising the following steps: S1. Mix the broken-cell pine pollen with lotus leaf powder to obtain the compound raw material; S2. Add deionized water to the compound raw materials, then add glucose, and then sterilize and cool. S3. Inoculate the cooled system with morel spore suspension at a rate of 5% to 15% (v / v) and ferment to obtain fermentation broth. S4. The fermentation broth is inactivated, then centrifuged to obtain the supernatant. The supernatant is concentrated and dried to obtain the pine pollen-lotus leaf composition based on morel fermentation.

[0012] The beneficial effects of the above-mentioned technical solution of the present invention are as follows: the preparation method of the present invention achieves efficient fermentation and conversion of pine pollen and lotus leaf composite substrate; specifically, using broken-cell-wall pine pollen as raw material is beneficial to the utilization and conversion of nutrients by morel mushrooms, and adding glucose as a carbon source promotes the growth and metabolism of morel mushrooms; sterilization treatment eliminates contamination by miscellaneous bacteria; and by controlling the inoculum amount, good initial growth conditions are provided for morel mushrooms. The method of the present invention is simple to operate, the conditions are controllable, and it is suitable for industrial production.

[0013] Furthermore, in step S2, the ratio of the compound raw material to deionized water is 1:10 to 1:25 (w / v), and the final concentration of glucose is 15 g / L to 25 g / L.

[0014] Furthermore, in step S2, the ratio of the compound raw material to deionized water is 1:20 (w / v), and the final concentration of glucose is 20 g / L.

[0015] Furthermore, in step S2, the sterilization process is pasteurization at a temperature of 80℃~90℃ for 20 minutes to 40 minutes.

[0016] Furthermore, in step S2, the sterilization process is high-pressure sterilization at a temperature of 105℃ to 121℃ for 5 to 20 minutes.

[0017] Furthermore, in step S3, the fermentation conditions are: temperature of 26±2℃, stirring speed of 120 rpm~200 rpm, and time of 3 to 7 days.

[0018] Furthermore, the concentration of the morel spore suspension is 1×10⁻⁶. 6 ~5×10 6 Spores / mL.

[0019] Furthermore, the inoculation amount of the morel spore suspension is 10% (v / v).

[0020] A third objective of this invention is to provide the use of the above-mentioned morel-fermented pine pollen-lotus leaf composition in the preparation of functional foods or health foods with weight loss or lipid-lowering functions.

[0021] Furthermore, the functional food or health food is in the form of a solid beverage, compressed candy, or capsule.

[0022] Compared with the prior art, the present invention has the following significant advantages: This invention provides the first known composition obtained through a morel-specific fermentation system of pine pollen and lotus leaf, exhibiting high activity with total phenols ≥ 5.5 mg GAE / g and total flavonoids ≥ 4.5 mg RE / g. Animal experiments have confirmed that this composition possesses weight-loss (23.5% reduction in body weight and 44.7% reduction in body fat percentage) and lipid-lowering (significantly reducing serum total cholesterol) effects comparable to the positive control drug orlistat, demonstrating clear and significant efficacy.

[0023] The preparation method of this invention is key to obtaining the above-mentioned composition. The raw material ratios, fermentation strain (morel), fermentation conditions, and other parameters in this method are all optimized to maximize the conversion and enrichment of active ingredients. Comparative studies have demonstrated that changing the strain or process cannot yield a composition with equivalent efficacy indicators.

[0024] The preparation process parameters of this invention are clear, the conditions are mild and controllable, and it is easy to achieve large-scale production. The resulting composition can be directly applied to various food forms and has broad application prospects. Attached Figure Description

[0025] Figure 1 Final body weight of mice in a high-fat diet-induced obese mouse model experiment; Figure 2 Fat body ratio in mice used in a high-fat diet-induced obese mouse model experiment; Figure 3 Cholesterol levels in mice used in a high-fat diet-induced obese mouse model experiment; Figure 4 Triglyceride levels in mice used in a high-fat diet-induced obese mouse model experiment; Figure 5 The level of high-density lipoprotein in mice used in a high-fat diet-induced obese mouse model experiment; Figure 6 Low-density lipoprotein levels in mice used in a high-fat diet-induced obese mouse model experiment; Figure 7 A stained photograph of a mouse liver section used in an experiment to demonstrate a mouse model of obesity induced by a high-fat diet. Detailed Implementation

[0026] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0027] Example 1 The preparation of a pine pollen-lotus leaf composition based on morel fermentation includes the following steps: S1. Weigh 200g of broken-cell wall pine pollen and 40g of lotus leaf powder (weight ratio 5:1) and mix them evenly.

[0028] S2. Add 4.8 L of deionized water (solid-to-liquid ratio 1:20, w / v) and 96 g of glucose (final concentration 20 g / L). Pasteurize in an 85°C water bath for 30 minutes, then cool to 26°C.

[0029] S3, Inoculate 480 mL of morel mushrooms ( Morchella sp. Spore suspension (approximately 1×10⁻⁶) purchased from the China Industrial Microbial Culture Collection Center, accession number CICC 14033. 6 Spores / mL, inoculum size 10% v / v. Fermented for 5 days at 26°C and 150 rpm.

[0030] S4. Inactivate the fermentation broth at 85℃ for 20 minutes, centrifuge (8000 rpm, 15 min), collect the supernatant, concentrate and freeze dry to obtain the freeze-dried powder of the composition.

[0031] Test results: The total phenol content of the composition was 5.57±0.10 mg GAE / g, the total flavonoid content was 4.50±0.35 mg RE / g, its free radical scavenging ability (DPPH) was 8.54±0.71 μmol / g, and the moisture content was 5.05%.

[0032] Examples 2-5 Examples 2-5 adjust parameters such as raw material ratio and liquid-to-material ratio (see Table 1 below), while other parameters and preparation processes are the same as in Example 1.

[0033] Table 1 Raw materials and preparation parameters for Examples 2-5

[0034] Comparative Example 1 The raw materials and their amounts in this comparative example are the same as in Example 1. The difference from Example 1 is that this comparative example is not inoculated with morel mushrooms, so no fermentation treatment is performed. Instead, it is directly centrifuged and freeze-dried after being pasteurized in an 85°C water bath for 30 minutes.

[0035] Comparative Example 2 Unlike Example 1, this comparative example uses Kluyveromyces lactis instead of Morchella esculenta. Kluyveromyces lactis Purchased from the China Industrial Microbial Culture Collection Center (accession number CICC 1572), the spore suspension contained approximately 1×10⁻⁶ spores. 7 Spores / mL, inoculum 10% (v / v), fermentation conditions 30℃, 200rpm, fermentation for 5 days, other parameters, raw materials and their amounts are the same as in Example 1.

[0036] Comparative Example 3 Unlike Example 1, this comparative example uses Rhizopus oryzae instead of Morel mushrooms. Rhizopus oryzae Purchased from the China Industrial Microbial Culture Collection Center (accession number CICC 3010), the spore suspension contained approximately 1×10⁻⁶ spores. 6 Spores / mL, inoculum 10% (v / v), fermentation conditions 30℃, 200rpm, fermentation for 5 days, other parameters, raw materials and their amounts are the same as in Example 1.

[0037] Comparative Example 4 Unlike Example 1, this comparative example only used 240g of pine pollen and did not add lotus leaf powder. The other raw materials and preparation parameters were the same as in Example 1.

[0038] The detection data results of Examples 1 to 5 and Comparative Examples 1 to 4 are shown in Table 2.

[0039] Table 2 Detection data results of Examples 1-5

[0040] Comparing Example 1 and Comparative Example 1, it can be seen that although the total flavonoid content of the unfermented compound is higher, its total phenolic content and DPPH free radical scavenging ability are significantly lower than those of the product fermented with morel mushrooms. This indicates that morel mushroom fermentation not only changes the composition ratio of active ingredients, but more importantly, it increases the content of phenolic substances with stronger antioxidant activity through biotransformation, which may be one of the reasons for its superior lipid-lowering effect in vivo. Comparing Example 1 and Comparative Example 4, the effect of fermenting pine pollen alone is very poor, proving that the combination with lotus leaf is essential for producing a highly active composition, and the two have a synergistic effect.

[0041] 3T3-L1 cell lipid accumulation inhibition experiment 1. Experimental Principle 3T3-L1 cells are a cell line derived from mouse embryonic fibroblasts. Under specific induction conditions, they can differentiate into mature adipocytes, form lipid droplets, and accumulate triglycerides. They are a classic in vitro model for studying adipogenesis and lipid metabolism.

[0042] 2. Experimental Materials 2.1 Experimental Cells 3T3-L1 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0043] 2.2 Test Sample The pine pollen-lotus leaf composition based on morel fermentation prepared in Example 1, the pine pollen-lotus leaf composition prepared in Comparative Example 1, and the pine pollen-lotus leaf composition based on yeast fermentation prepared in Comparative Example 2.

[0044] 2.3 Reagents and Materials High glucose DMEM medium, fetal bovine serum (FBS), penicillin-streptomycin solution (P / S), 0.25% trypsin-EDTA digestion solution, 3-isobutyl-1-methylxanthine, dexamethasone and insulin were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0045] 4% paraformaldehyde fixative, 60% isopropanol differentiation solution, and phosphate buffered saline (PBS) were purchased from Anhui Baisha Biotechnology Co., Ltd.

[0046] The triglyceride quantitative assay kit was purchased from Nanjing Jiancheng Biotechnology Co., Ltd.

[0047] Other cell culture consumables, including T25 / T75 culture flasks, 6-well or 12-well cell culture plates, and 15mL and 50mL centrifuge tubes, were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0048] 3. Experimental Methods 3.1 3T3-L1 cell culture and induction of differentiation 3T3-L1 cells were cultured and proliferated in high-glucose DMEM medium supplemented with 10% FBS and 1% P / S at 37°C, 5% CO2, and under a humid atmosphere. Cell differentiation and adipogenesis were induced using the classic "cocktail method." Two days after cell confluence, 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM dexamethasone, and 10 μg / ml insulin were added to the complete culture medium and maintained for two days. The inducing agent was then replaced with 10 μg / ml insulin, and the medium was changed every two days until mature adipocytes accounted for more than 80% of the cells by days 8-12.

[0049] The lyophilized fermentation samples from Examples 1, 1, and 2 were reconstituted with PBS to prepare 40 mg / mL stock solutions, which were then sterilized by filtering through a 0.22 μm filter. The stock solutions were then diluted to 200 μg / mL and administered to cells from day four to day eight.

[0050] 3.2 Inhibition of Triglyceride (TG) Accumulation Cells induced for differentiation for 10 days in 6-well plates were collected, and the culture supernatant was discarded. The surface of the cells was gently washed twice with PBS, and the cells were removed by pipetting and centrifugation. Cells in each well were lysed with 100 μL of RIPA lysis buffer, and the TG content was determined using a TG assay kit.

[0051] 3.3 Statistical Methods Data are expressed as mean ± standard deviation, and all treatments were repeated six times. Excel 2019 was used for data analysis. P <0.05 indicates a significant difference.

[0052] 4. Experimental Results 4.1 Effects of different fermentation products on the inhibition of lipid accumulation in 3T3-L1 cells The inhibitory effects of different fermentation products on cellular lipid accumulation are shown in Table 3.

[0053] Table 3. Different fermentation products inhibit lipid accumulation (n=6)

[0054] Compared with the unfermented compound (Comparative Example 1), both fermented products significantly inhibited lipid accumulation. P <0.01). More importantly, the inhibitory effect of the morel ferment (Example 1) was significantly better than that of the yeast ferment (Comparative Example 2). P <0.01), demonstrating the unique advantages of morel fermentation at the cellular level.

[0055] High-fat diet-induced obese mouse model experiment 1. Experimental Principle This experiment used a high-fat diet to induce an obese mouse model to investigate the weight loss and lipid-lowering effects of the formulation on obese mice.

[0056] 2. Experimental Materials 2.1 Laboratory Animals Five-week-old male C57BL / 6J mice were purchased from Zhejiang Vital River Laboratory Animal Technology Co., Ltd. All mice were acclimatized to a 12-hour light / 12-hour dark cycle at 23±1℃ and 55%±5% relative humidity, with free access to food and water.

[0057] 2.2 Test Sample The pine pollen-lotus leaf composition based on morel fermentation prepared in Example 1 (referred to as Example Group), the pine pollen-lotus leaf composition based on yeast fermentation prepared in Comparative Example 2 (referred to as Comparative Group), and orlistat (referred to as Positive Control Group).

[0058] 2.3 Reagents and Materials 60% high-fat feed (TP23300, protein calories 19%, carbohydrates 21%, fat 60.0%). Control diet (TP23302, protein calories 19%, carbohydrates 71%, fat 10.0%). All the above feeds were purchased from Nantong Trofi Feed Technology Co., Ltd.

[0059] Orlistat was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0060] Paraformaldehyde (4%) was purchased from Anhui Baisha Biotechnology Co., Ltd.

[0061] Hematoxylin and eosin (H&E) staining solution was purchased from Beyotime Biotechnology Co., Ltd.

[0062] The total cholesterol (TG) assay kit, triglyceride (TC) assay kit, high-density lipoprotein cholesterol (HDL-C) assay kit, and low-density lipoprotein cholesterol (LDL-C) assay kit were all purchased from Nanjing Jiancheng Biotechnology Co., Ltd.

[0063] 3. Experimental Methods 3.1 Animal Grouping Five-week-old male C57BL / 6J mice were randomly and blinded, with eight mice in each group. There were five groups in total: normal control group (blank control group), model group, positive control group, example control group, and comparative control group.

[0064] 3.2 Animal Experiment Methods The normal group was fed a control diet, while the other groups were fed a diet containing 60% high-fat to establish a high-fat diet-induced obese mouse model.

[0065] Starting from the first day of feeding, medication was administered simultaneously. All samples were dissolved in physiological saline and administered via gavage at a rate of 0.2 mL once daily. The dosages were as follows: positive control group (60 mg / kg bw), example group (400 mg / kg bw), and comparative group (400 mg / kg bw). The normal control group (blank control group) and the model group received 0.2 mL of physiological saline via gavage daily. The experimental period was 13 weeks.

[0066] 3.3 Weight Mice weight was recorded weekly.

[0067] 3.4 Measurement of mouse serum markers Mouse serum was collected to measure the levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). The measurement methods were performed according to the kit instructions. Results are as follows: Figure 3-6 As shown.

[0068] 3.5 Histopathological Analysis Fresh mouse adipose tissue was washed with PBS and fixed with 4% paraformaldehyde. After dehydration, paraffin embedding, and sectioning, the morphology of the adipose tissue was observed by staining with hematoxylin and eosin (H&E). Results are as follows: Figure 7 As shown.

[0069] 3.6 Statistical Methods Data are expressed as mean ± standard deviation, and all treatments were repeated three times. Excel 2019 was used for data analysis, and GraphPad Prism 9.5.1 was used for plotting and significance analysis. P <0.05 indicates a significant difference.

[0070] 4. Experimental Results 4.1 Effects of morel-fermented pine pollen-lotus leaf composition on body weight in high-fat diet-induced obese mice See Figure 1 Compared with the normal group (blank control group), the body weight of mice in the high-fat diet-induced model group increased by about 38.3%. After treatment with the composition of Example 1 and the positive control drug (orlistat), the body weight of mice decreased significantly compared with the model group, by about 23.4% and 23.5% respectively. There was no significant difference between the positive control group and the example group.

[0071] See Figure 2 The fat body ratio of mice in the high-fat diet-induced model group was significantly increased compared with that of the normal group. Compared with the model group, the fat body ratio of mice in the example group and the positive control group was significantly decreased after intervention. This indicates that the composition of the present invention and the positive control drug (orlitol) have a good effect on improving the fat body ratio of mice induced by high-fat diet. Among them, the pine pollen-lotus leaf composition based on morel fermentation is comparable to the positive control drug, and there is no significant difference between them.

[0072] 4.2 Effects of morel-fermented pine pollen-lotus leaf composition on serum markers in high-fat diet-induced obese mice See Figure 3The results of high-fat diet-induced obese mice showed that the serum TC level in the model group was significantly higher than that in the normal group. P <0.05), compared to the model group, the serum TC content in obese mice was significantly reduced after intervention in both the positive control group and the example group. Specifically, the example group significantly reduced serum TC content in obese mice by approximately 24.85%, while the positive control group reduced it by approximately 16.11%. These results indicate that the composition used in the example group has a good effect on improving serum total cholesterol in obese mice.

[0073] See Figure 4-6 The results of serum triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) showed that the morel-based pine pollen-lotus leaf composition of the example improved serum TG, HDL-C and LDL-C in obese mice induced by a high-fat diet, but there was no significant difference compared with the positive control group.

[0074] 4.3 Effects of morel-fermented pine pollen-lotus leaf composition on liver tissue of high-fat diet-induced obese mice HE staining results of mouse liver tissue sections ( Figure 7 The results showed that the liver cells of the normal group mice were of relatively uniform size, with clearly visible nuclei, relatively uniform cytoplasm, clear outlines of liver lobules and sinusoids, and neatly arranged liver cords, indicating that the liver tissue structure was not damaged. The liver cells of the model group mice had large lipid droplets and vacuoles, indicating fatty degeneration. Compared with the model group, the livers of the positive control group and the example group (pine pollen + lotus leaf morel fermentation group) mice had only a few small lipid droplets, which had a good effect on improving liver fatty degeneration.

[0075] The above results indicate that the pine pollen and lotus leaf compound morel fermented extract prepared in Example 1 has a weight loss and lipid-lowering effect similar to orlistat, and is a health food composition that can assist in weight loss and lipid-lowering.

[0076] 4.4 Effects of fermentation complexes from different strains on key obesity indicators in high-fat diet-induced obese mice The test results of each key obesity indicator parameter are shown in Table 4.

[0077] Table 4 Comparison of key obesity indicators in mice (n=8)

[0078] Note: In Table 4, # indicates the comparison with the normal group. P <0.05; ## indicates the difference compared to the normal group P <0.01; ### indicates the ratio compared to the normal group P <0.001; Indicates the ratio to the model groupP <0.05; Indicates the ratio to the model group P <0.01; Indicates the ratio to the model group P <0.001.

[0079] Compared with Example 1 (Example Group) and Comparative Example 2 (Comparative Group), although yeast fermentation has advantages in increasing total flavonoids and in vitro antioxidant (DPPH), morel fermentation showed significantly better and unexpected effects in inhibiting cellular lipid accumulation and in the core efficacy indicators of overall animal weight loss and lipid reduction (body weight, fat body ratio, TC, TG). This indicates that different bacterial species have different metabolite profiles, and morel produces active substances that are more targeted to the obesity metabolic pathway.

[0080] This invention provides, for the first time, a composition with high levels of active ingredients (total phenols ≥ 5.5 mg GAE / g, total flavonoids ≥ 4.5 mg RE / g) obtained from morel fermented pine pollen and lotus leaves. Animal experiments have demonstrated that this composition has significant weight-loss and lipid-lowering effects. This invention also provides a method for preparing this composition and its uses, forming a complete technical solution with promising industrialization prospects.

[0081] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A pine pollen-lotus leaf composition based on morel fermentation, characterized in that, It is prepared by fermenting a compound substrate of pine pollen and lotus leaf using morel mushrooms, wherein the weight ratio of pine pollen to lotus leaf is 3 to 6:

1.

2. A method for preparing the pine pollen-lotus leaf composition based on morel fermentation as described in claim 1, characterized in that, Includes the following steps: S1. Mix the broken-cell pine pollen with lotus leaf powder to obtain the compound raw material; S2. Add deionized water to the compound raw materials, then add glucose, and then sterilize and cool. S3. Inoculate the cooled system with morel spore suspension at a rate of 5% to 15%, and ferment to obtain fermentation broth. S4. The fermentation broth is inactivated, then centrifuged to obtain the supernatant. The supernatant is concentrated and dried to obtain the pine pollen-lotus leaf composition based on morel fermentation.

3. The method for preparing the pine pollen-lotus leaf composition based on morel fermentation according to claim 2, characterized in that, In step S2, the ratio of the compound raw material to deionized water is 1:10 to 1:25, and the final concentration of glucose is 15g / L to 25g / L.

4. The method for preparing the pine pollen-lotus leaf composition based on morel fermentation according to claim 2, characterized in that, In step S2, the sterilization process is pasteurization at a temperature of 80℃~90℃ for 20 minutes to 40 minutes.

5. The method for preparing the pine pollen-lotus leaf composition based on morel fermentation according to claim 2, characterized in that, In step S2, the sterilization process is high-pressure sterilization at a temperature of 105℃ to 121℃ for 5 to 20 minutes.

6. The method for preparing the pine pollen-lotus leaf composition based on morel fermentation according to claim 2, characterized in that, In step S3, the fermentation conditions are: temperature 26±2℃, stirring speed 120 rpm~200 rpm, and time 3 to 7 days; the concentration of the morel spore suspension is 1×10⁻⁶. 6 ~5×10 6 Spores / mL.

7. The use of the morel-fermented pine pollen-lotus leaf composition according to claim 1 in the preparation of functional foods or health foods with weight loss or lipid-lowering functions.

8. The use according to claim 7, characterized in that: The functional food or health food is in the form of solid beverage, compressed candy, or capsule.

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