Composition with function of preventing hair breakage as well as preparation method and application of composition
By combining plant extracts with complex peptides, this product improves the scalp microenvironment, activates hair follicle function, and strengthens hair structure, overcoming the limitations of existing anti-breakage products and achieving significant anti-breakage effects and safety.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGDONG FADI COSMETICS TECH CO LTD
- Filing Date
- 2026-02-02
- Publication Date
- 2026-05-12
AI Technical Summary
Existing anti-hair breakage products cannot systematically address issues such as scalp microenvironment disorder, insufficient nutrient supply to hair follicles, and brittle internal hair structure. They also pose a risk of scalp sensitivity or inflammatory reactions due to chemical components and lack a multi-target synergistic mechanism, resulting in short-lived efficacy.
The product utilizes a combination of plant extracts and complex peptides, including Panax notoginseng root extract, Cnidium monnieri fruit extract, coral algae extract, and tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3. By improving the scalp microenvironment, activating hair follicle function, and enhancing hair structure, it provides a multi-target synergistic effect.
It achieves significant anti-breakage effects, enhances hair's tensile strength, is gentle on the scalp and does not irritate it, builds a basic barrier against breakage, and promotes hair follicle activation and hair strengthening.
Abstract
Description
Technical Field
[0001] This invention relates to the field of daily cosmetics, specifically to a composition with anti-hair breakage function, its preparation method, and its application. Background Technology
[0002] As consumers place increasing emphasis on hair health, anti-breakage technology has become a key research area in personal care. Traditional anti-breakage products often focus on improving the surface condition of hair strands through methods such as silicone oil coating, oil lubrication, or physical pulling, but these methods have significant limitations: Firstly, these products primarily target the surface layer of the hair strand, failing to systematically address deeper issues such as scalp microenvironment imbalance, insufficient nutrient supply to hair follicles, and brittleness of the internal hair structure. Secondly, some formulas contain irritating chemical components (such as sulfate surfactants and high-concentration preservatives), which can easily trigger scalp sensitivity or inflammatory reactions, potentially exacerbating hair damage with long-term use. Furthermore, existing technologies often rely on single active ingredients (such as keratin peptides and plant oils), lacking a multi-target synergistic mechanism, resulting in short-lived effects and difficulty in sustainably improving hair quality. Especially for core issues such as insufficient hair supply due to hair follicle degeneration and follicular inflammation caused by scalp oxidative stress, current technologies have not yet formed effective comprehensive solutions. A breakthrough in a systematic anti-breakage technology that integrates scalp microecological regulation, hair follicle function activation, and hair strengthening is urgently needed. Summary of the Invention
[0003] The first technical problem to be solved by the present invention is to provide a composition with significant efficacy and no side effects that prevents hair breakage, in order to address the shortcomings of the existing technology.
[0004] The second technical problem to be solved by the present invention is to provide a composition with anti-hair breakage function for use in shampoo and hair care products, in view of the shortcomings of the existing technology.
[0005] The third technical problem to be solved by the present invention is to provide a hair care essence with anti-breakage effect and its preparation method, in view of the shortcomings of the existing technology.
[0006] To solve the first technical problem mentioned above, the technical solution of the present invention is as follows: This invention provides a composition with anti-hair breakage effect, the composition comprising the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and Coral algae extract, with a mass ratio of 1:(3-5):(1-3); the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:(0.5-0.7):(0.2-0.4); the mass ratio of the plant extracts to the complex polypeptides is 1:(0.01-0.5).
[0007] Preferably, the composition comprises the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and Coral algae extract, with a mass ratio of 1:(3.5-4.5):(1.5-2.5); the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:(0.6-0.7):(0.2-0.3); and the mass ratio of the plant extracts to the complex polypeptides is 1:(0.05-0.1).
[0008] More preferably, the composition comprises the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract, with a mass ratio of 1:4:2; the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:0.6:0.3; and the mass ratio of the plant extracts to the complex polypeptides is 1:0.1.
[0009] To solve the second technical problem mentioned above, the present invention provides an application of the above-mentioned composition with anti-hair breakage effect in shampoo and hair care products.
[0010] Preferably, the shampoo and hair care products include any one of shampoo, conditioner, hair serum, and hair mask.
[0011] To solve the third technical problem mentioned above, the present invention provides a hair care essence with anti-breakage effect, the hair care essence comprising the above-mentioned composition with anti-breakage effect.
[0012] Preferably, the hair care essence further includes oils, polyols, deionized water, pH adjusters, thickeners, preservatives, chelating agents, and fragrances.
[0013] Preferably, the oils in the hair care essence include at least one of jojoba seed oil, grape seed oil, meadowfoam seed oil, babassu seed oil, macadamia seed oil, tea seed oil, and plantain seed oil.
[0014] Preferably, the polyol in the hair care essence includes at least one of glycerin, 1,2-hexanediol, caprylyl glycol, ethylhexylglycerin, propylene glycol, glyceryl glucoside, pentaerythritol, and inositol.
[0015] Preferably, the thickener in the hair care essence includes at least one of hydroxyethyl cellulose, xanthan gum, hydroxypropyl methylcellulose, and carbomer; Preferably, the preservative in the hair care essence includes at least one of sodium benzoate, methylparaben, and potassium sorbate; Preferably, the chelating agent in the hair care essence includes at least one of EDTA-disodium, sodium tripolyphosphate, and capryloyl hydrovalerate; Preferably, the pH adjuster in the hair care essence includes at least one of citric acid and lactic acid; Preferably, the preparation method of the hair care essence includes the following steps: S1: Heat polyol, chelating agent, composition with anti-hair breakage effect, and deionized water to 60-70℃, stir evenly, and keep warm for 60-80 minutes to obtain phase A; S2: Mix the oil and fat evenly to obtain phase B; S3: After phase A cools down to 40-50℃, add it to phase B and stir evenly to obtain mixed phase C; S4: After the mixed phase C is cooled to 30-35℃, add the thickener, preservative and fragrance to the mixed phase C, stir evenly to obtain the mixed phase D; S5: Use a pH adjuster to adjust the pH of the mixed phase D to 5.5-6.5, stir evenly, and let stand for 30-60 minutes; then discharge.
[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention innovatively provides a composition with anti-breakage effects, comprising plant extracts and complex peptides; wherein the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract; the complex peptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3; the various raw materials in the anti-breakage composition obtained by this invention work synergistically to achieve significant anti-breakage effects, enhance hair tensile strength, and are gentle and non-irritating to the scalp. The principle may be as follows: 1. Improves the scalp microenvironment and builds a basic barrier against hair breakage. The saponins contained in Panax notoginseng root extract can reduce oxidative stress or damage to hair follicles by inhibiting free radical activity and downregulating the expression of inflammatory factors. Osthol in Cnidium monnieri fruit extract has a broad-spectrum anti-inflammatory effect. When used in combination with Panax notoginseng root extract, it can synergistically reduce scalp inflammation by inhibiting different inflammatory pathways. In addition, the natural mildness of Cnidium monnieri fruit extract can enhance the non-irritating properties of the combination.
[0017] 2. Activates hair follicle function, promoting stronger hair at the source. Coral algae extract is rich in collagen peptides and various trace elements, which can act as a cell activator to provide essential nutrients for hair follicles and provide the raw material basis for hair growth.
[0018] The complex peptides can activate the proliferation and migration of dermal papilla cells, promote the hair follicles from the resting phase to the growth phase, and enhance the activity of hair follicles. The six ingredients mentioned above work synergistically across multiple targets—scalp microenvironment repair, hair follicle function activation, and hair strand structure strengthening—to enhance hair's tensile strength and prevent breakage. Simultaneously, the combination of natural plant extracts and gentle active peptides ensures a non-irritating effect on the scalp. This synergistic mechanism not only covers the entire chain of hair breakage prevention (from scalp to hair follicles to hair strands) but also prioritizes safety, making it a highly effective and gentle solution for preventing hair breakage.
[0019] The present invention will be further illustrated below with reference to specific compositions, embodiments, and applications. It should be understood that these compositions, embodiments, and applications are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.
[0020] The raw materials in the composition of this invention are sourced from the following sources: Panax notoginseng root extract was purchased from Nanjing Dausif Biotechnology Co., Ltd. Cnidium monnieri fruit extract, purchased from Shaanxi Gerun Biotechnology Co., Ltd.; Coral algae extract, purchased from Wuhan Kemic Biomedical Technology Co., Ltd.; Tripeptide-2 was purchased from Xi'an Puris Biotechnology Co., Ltd. Acetyl tetrapeptide-3, purchased from Hubei Xinyuhong Biomedical Technology Co., Ltd.; Pentapeptide-3 was purchased from Hubei Xinjiecheng Chemical Technology Co., Ltd. Unless otherwise specified, all other materials and reagents used in the experiments are commercially available.
[0021] A composition with anti-hair breakage function, wherein the raw materials, mass ratios, and preparation method of the composition are as follows: Composition 1: The mass ratio of plant extract to complex polypeptide is 1:0.1; The plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract, wherein the mass ratio of Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract is 1:4:2. The complex polypeptide includes tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, wherein the mass ratio of tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 is 1:0.6:0.3; Preparation method of composition 1: S1: Weigh each component of the plant extract precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase A; S2: Weigh each component of the complex polypeptide precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase B; S3: Mix phase A and phase B at 25°C according to the above formula ratio to obtain a composition with anti-hair breakage function.
[0022] Composition 2: The mass ratio of plant extract to complex polypeptide is 1:0.01; The plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract, with the mass ratio of Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract being 1:3:1. The complex polypeptide includes tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, wherein the mass ratio of tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 is 1:0.5:0.2. Preparation method of composition 2: S1: Weigh each component of the plant extract precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase A; S2: Weigh each component of the complex polypeptide precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase B; S3: Mix phase A and phase B at 25°C according to the above formula ratio to obtain a composition with anti-hair breakage function.
[0023] Composition 3: The mass ratio of plant extract to complex polypeptide is 1:0.5; The plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract, with the mass ratio of Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract being 1:5:3. The complex polypeptide includes tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, wherein the mass ratio of tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 is 1:0.7:0.4. Preparation method of composition 3: S1: Weigh each component of the plant extract precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase A; S2: Weigh each component of the complex polypeptide precisely according to the above formula ratio, mix them evenly at 25℃ to obtain phase B; S3: Mix phase A and phase B at 25°C according to the above formula ratio to obtain a composition with anti-hair breakage function.
[0024] Composition ①: Unlike composition 1, this composition lacks a complex polypeptide, and the missing component is made up by an equal mass of plant extract, wherein the raw materials and mass ratio of the plant extract are the same as those in composition 1; the remaining components and mass ratios are the same as those in composition 1.
[0025] Preparation method of composition ①: S1: Weigh each component of the plant extract precisely according to the above formula ratio, mix them evenly at 25°C to obtain a composition with anti-hair breakage function.
[0026] Composition ②: Unlike composition 1, this composition lacks plant extracts, and the missing components are made up by an equal mass of complex polypeptides, wherein the raw materials and mass ratios of the complex polypeptides are the same as those in composition 1; the remaining components and mass ratios are the same as those in composition 1.
[0027] Preparation method of composition ②: S1: Weigh each component of the compound polypeptide precisely according to the above formula ratio, mix them evenly at 25°C to obtain a composition with anti-hair breakage function.
[0028] Composition ③: Unlike composition 1, the mass ratio of plant extract to complex polypeptide is 1:1; the raw materials and mass ratio of plant extract and complex polypeptide are the same as those in composition 1. Composition ③ is prepared in the same way as composition 1.
[0029] Composition ④: Unlike Composition 1, the plant extract lacks Panax notoginseng root extract. The missing mass fraction is made up by Cnidium monnieri extract and coral algae extract in a mass ratio of 4:2. The other raw materials and mass ratios are the same as those in Composition 1. Composition ④ is prepared in the same way as composition 1.
[0030] Composition ⑤: Unlike composition 1, the plant extract lacks Cnidium monnieri extract. The missing mass is made up by Panax notoginseng root extract and coral algae extract in a mass ratio of 1:2. The other raw materials and mass ratios are the same as those in composition 1. Composition ⑤ is prepared in the same way as composition 1.
[0031] Composition ⑥: Unlike composition 1, the plant extract lacks coral algae extract. The missing mass is made up by Panax notoginseng root extract and Cnidium monnieri fruit extract in a mass ratio of 1:4. The other raw materials and mass ratios are the same as those in composition 1. Composition ⑥ is prepared in the same way as composition 1.
[0032] Composition ⑦: Unlike composition 1, the plant extract is composed of Panax notoginseng root extract, Cnidium monnieri fruit extract and Cnidium monnieri fruit extract in a mass ratio of 1:1:4, and the remaining raw materials and mass ratios are the same as those in composition 1. Composition ⑦ is prepared in the same way as composition 1.
[0033] Composition ⑧: Unlike composition 1, the complex polypeptide lacks tripeptide-2. The missing mass fraction is made up by acetyl tetrapeptide-3 and pentapeptide-3 in a mass ratio of 0.6:0.3. The other raw materials and mass ratios are the same as those in composition 1. Composition ⑧ is prepared in the same way as composition 1.
[0034] Composition 9: Unlike composition 1, the complex polypeptide lacks acetyl tetrapeptide-3. The missing mass is made up by tripeptide-2 and pentapeptide-3 in a mass ratio of 1:0.3. The other raw materials and mass ratios are the same as those in composition 1. Composition 9 is prepared in the same way as composition 1.
[0035] Composition ⑩: Unlike composition 1, the complex polypeptide lacks pentapeptide-3. The missing mass fraction is made up by tripeptide-2 and acetyl tetrapeptide-3 in a mass ratio of 1:0.6. The other raw materials and mass ratios are the same as those in composition 1. The preparation method of composition ⑩ is the same as that of composition 1.
[0036] Composition ⑪: Unlike composition 1, the complex polypeptide is composed of tripeptide-2, acetyl tetrapeptide-3 and pentapeptide-3 in a mass ratio of 1:0.3:0.6, and the remaining raw materials and mass ratios are the same as those in composition 1. The preparation method of composition ⑪ is the same as that of composition 1.
[0037] A hair care essence with anti-breakage effect, wherein the raw material mass percentage and preparation method of the hair care essence are as follows: Hair serum 1: Composition 1:5wt% Polyols: 20wt% Chelating agent: 1 wt% Oils: 10wt% Thickener: 0.5 wt%; Preservative: 0.1 wt%; Fragrance: 0.08 wt% pH adjuster: appropriate amount; Deionized water to 100 wt%; The polyol is glycerol; the chelating agent is disodium EDTA; the oil is grape seed oil; the thickener is carbomer; the preservative is potassium sorbate; and the pH adjuster is citric acid.
[0038] The preparation method of the hair care essence 1 includes the following steps: S1: Heat the polyol, chelating agent, composition, and deionized water to 65°C, stir until homogeneous, and keep warm for 70 minutes to obtain phase A; S2: Mix the oil and fat evenly to obtain phase B; S3: After phase A cools down to 45°C, add it to phase B and stir evenly to obtain mixed phase C; S4: After the mixed phase C is cooled to 35℃, add the thickener, preservative and flavoring to the mixed phase C, stir evenly to obtain the mixed phase D; S5: Use a pH adjuster to adjust the pH of the mixed phase D to 6.0, stir evenly, and let stand for 40 minutes; then discharge.
[0039] Hair serum 2: Unlike hair essence 1, composition 2 is used to replace composition 1 in an equal amount, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0040] Hair serum 3: Unlike hair essence 1, composition 3 is used to replace composition 1 in an equal amount, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0041] Hair care essence ①: Unlike hair essence 1, composition ① is used to replace composition 1 in an equal amount, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0042] Hair care essence ②: Unlike hair essence 1, composition 2 is used to replace composition 1 in an equal amount, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0043] Hair care essence ③: Unlike hair essence 1, composition 3 is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0044] Hair care essence ④: Unlike hair essence 1, composition 4 is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0045] Hair care essence ⑤: Unlike hair essence 1, composition 5 is used to replace composition 1 in an equal amount, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0046] Hair care essence ⑥: Unlike hair essence 1, composition 6 is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0047] Hair care essence ⑦: Unlike hair essence 1, composition 7 is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0048] Hair serum ⑧: Unlike hair essence 1, composition ⑧ is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0049] Hair care essence 9: Unlike hair essence 1, composition 9 is used to replace composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0050] Hair serum ⑩: Unlike hair essence 1, composition 1 is replaced by composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0051] Hair care essence ⑪: Unlike hair essence 1, composition 1 is replaced by composition 1 in equal amounts, while the remaining raw materials, mass percentages, and preparation methods are the same as those for hair essence 1.
[0052] Hair serum blank: Polyols: 20wt% Chelating agent: 1 wt% Oils: 10wt% Thickener: 0.5 wt%; Preservative: 0.1 wt%; Fragrance: 0.08 wt% pH adjuster: appropriate amount; Deionized water to 100 wt%; The polyol is glycerol; the chelating agent is disodium EDTA; the oil is grape seed oil; the thickener is carbomer; the preservative is potassium sorbate; and the pH adjuster is citric acid.
[0053] The method for preparing the blank of the hair care essence includes the following steps: S1: Heat polyol, chelating agent, and deionized water to 65°C, stir evenly, and keep warm for 70 minutes to obtain phase A; S2: Mix the oil and fat evenly to obtain phase B; S3: After phase A cools down to 45°C, add it to phase B and stir evenly to obtain mixed phase C; S4: After the mixed phase C is cooled to 35℃, add the thickener, preservative and flavoring to the mixed phase C, stir evenly to obtain the mixed phase D; S5: Use a pH adjuster to adjust the pH of the mixed phase D to 6.0, stir evenly, and let stand for 40 minutes; then discharge.
[0054] Efficacy test: Experiment 1: Effect of the composition on the viability of human dermal papilla cells Test samples: Compositions 1-3 and ①-⑪ with a concentration of 1 mg / mL, in DMEM (FBS-free, antibiotic-free) medium.
[0055] Experimental Methods: Human dermal papilla cells (purchased from Shanghai Lianmai Biotechnology Co., Ltd., catalog number LM-H005) nearing the logarithmic growth phase were selected (cell confluence reached 70%-80%). The supernatant was discarded using a disposable dropper, and the cells were washed 2-3 times with PBS. Approximately 2 mL of trypsin was added (for plating), and the cells were digested at 37°C for 3-5 minutes. Cells were pipetted, and immediately after pipetting, complete culture medium (DMEM++, containing FBS and antibiotics) was added to terminate the reaction. The cells were collected into 15 mL sterile centrifuge tubes and centrifuged at 1000 rpm for 5 minutes. The supernatant was slowly aspirated and discarded, and an appropriate amount of DMEM++ was added to resuspend the cells. Cell counts were performed, and the cells were collected at a rate of 1.5 × 10⁻⁶ cells / mL. 5 Inoculate at a density of 1 mL / well into 24-well plates and incubate at 37°C in a 5% CO2 incubator for 24 h.
[0056] Remove DMEM++ medium and wash twice with phosphate-buffered saline (PBS), aspirating any residual liquid during washing. For the experimental group, add 100 μL of the prepared test sample to each well and mix. For the control group, use an equal volume of DMEM (FBS-free, antibiotic-free) medium instead of the test sample. Both experimental and control groups were repeated three times. Incubate at 37℃ in a 5% CO2 incubator for 24 h. After 24 h of cell culture, carefully aspirate the culture medium and wash twice with PBS. Add 100 μL / well of 0.5 mg / mL MTT solution. Continue culturing for 4 h, then aspirate the MTT solution. Add 150 μL / well of DMSO and shake slowly for 10 min using a microplate reader to fully dissolve any crystals. Measure OD490 using a microplate reader. Calculate the cell proliferation rate of human dermal papilla cells using the following formula: Cell proliferation rate (%) = [(Experimental group absorbance value / Control group absorbance value) - 1] × 100%.
[0057] Table 1 Effects of the composition on human dermal papilla cells Group Cell proliferation rate / % Composition 1 87.42 Composition 2 79.37 Composition 3 89.94 Composition ① 45.31 Composition ② 69.26 Composition ③ 61.42 Composition ④ 53.29 Composition ⑤ 51.31 Composition ⑥ 55.42 Composition ⑦ 70.33 Composition ⑧ 50.22 Composition 9 53.94 Composition ⑩ 51.05 Composition ⑪ 60.37 As shown in Table 1, compositions 1-3 and ①-⑪ all exhibit certain effects in promoting the proliferation rate of human dermal papilla cells. Comparing the results of composition 1 with those of compositions ①-②, the compound polypeptide defined in this invention shows significantly better efficacy in promoting the proliferation rate of human dermal papilla cells than the plant extracts. However, the combined effect of both, using the mass ratio defined in this invention, is more significant, indicating a significant synergistic effect between the plant extracts and the compound polypeptide. Comparing the results of composition 1 with those of compositions ③-⑥, the extracts of Panax notoginseng root, Cnidium monnieri fruit, and Coral Algae in the compositions of this invention exhibit a significant synergistic effect with the compound polypeptide, and their combined use can significantly enhance the effect of the compositions on human dermal papilla cell proliferation. Nipple proliferation rate; Comparing the results of composition 1 and composition 7, it can be seen that the extracts of Panax notoginseng root, Cnidium monnieri fruit, and Coral algae have the best efficacy in promoting the proliferation rate of human dermal papilla cells within the mass ratio range specified in this invention; Comparing the results of composition 1 and compositions 8-10, it can be seen that tripeptide-2, acetyl tetrapeptide-3, pentapeptide-3 in the compound polypeptide of this invention have a significant synergistic effect with the plant extracts, and their combined use can significantly improve the proliferation rate of human dermal papilla cells; Comparing the results of composition 1 and composition 11, it can be seen that tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 have the strongest synergistic effect with the plant extracts within the mass ratio range specified in this invention, and have the best effect in promoting the proliferation rate of human dermal papilla cells.
[0058] Experiment 2: Inhibitory effect of the composition on DPPH free radicals Sample solution preparation: Prepare sample solutions of compositions 1-3 and compositions ①-⑪ to a concentration of 1 mg / mL using deionized water, and prepare fresh each time.
[0059] Weigh 12 mg of DPPH powder and dissolve it in 100 ml of anhydrous ethanol. Sonicate the solution for 30 min to prepare the DPPH free radical working solution. DPPH free radicals are photosensitive and easily affected by environmental oxidation; therefore, the DPPH working solution should be prepared fresh before use. Add 100 μL of sample solution to a 96-well plate, add 100 μL of DPPH working solution, and react at room temperature for 30 min. Measure the absorbance AX at 517 nm using a microplate reader. Measure the absorbance A0 of the solution after mixing 100 μL of DPPH powder with 100 μL of anhydrous ethanol. Measure the absorbance A1 of the solution after mixing 100 μL of deionized water with 100 μL of DPPH working solution. Each experiment should be performed in triplicate. Calculate the DPPH scavenging rate using the following formula.
[0060] DPPH clearance rate % = [1 - (AX - A0) / A1] × 100% AX: Absorbance of 100 μL sample + 100 μL DPPH; A0: Absorbance of 100 μL sample + 100 μL anhydrous ethanol; A1: Absorbance of 100 μL deionized water + 100 μL DPPH.
[0061] Table 2. Determination of the antioxidant efficacy of the composition Group DPPH removal rate / % Composition 1 65.72 Composition 2 67.44 Composition 3 59.31 Composition ① 55.26 Composition ② 19.31 Composition ③ 45.33 Composition ④ 32.42 Composition ⑤ 42.56 Composition ⑥ 40.15 Composition ⑦ 50.31 Composition ⑧ 45.22 Composition 9 47.63 Composition ⑩ 46.24 Composition ⑪ 55.34 As shown in Table 2, compositions 1-3 and compositions ①-⑪ all possess certain antioxidant effects. Comparing the results of composition 1 with those of compositions ①-②, the antioxidant effects of the plant extracts specified in this invention are significantly superior to those of the complex peptides. However, the combined antioxidant effect achieved by using both in the mass ratio specified in this invention is more significant, indicating a significant synergistic effect between the plant extracts and the complex peptides. Comparing the results of composition 1 with those of compositions ③-⑥, the extracts of Panax notoginseng root, Cnidium monnieri fruit, and Coral Algae in the compositions of this invention exhibit a significant synergistic effect with the complex peptides, and their combined use can significantly enhance the antioxidant effect of the compositions. The antioxidant capacity of the extracts was compared with that of composition 7. The results showed that the extracts of Panax notoginseng root, Cnidium monnieri fruit, and Coral Algae exhibited the best antioxidant efficacy within the mass ratio range specified in this invention. The results of composition 1 with compositions 8-10 showed that tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 in the compound polypeptide of this invention had a significant synergistic effect with the plant extracts, and their combined use significantly enhanced the antioxidant capacity of the composition. The results of composition 1 with composition 11 showed that the synergistic effect of tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 with the plant extracts was the strongest and the antioxidant efficacy was the best within the mass ratio range specified in this invention.
[0062] Test 3: Safety test of the composition and hair care essence Test samples: Compositions 1-3, ①-⑪; hair essence 1-3, ①-⑪, blank hair essence; wherein the compositions were prepared into a 30wt% mixture using deionized water.
[0063] Patch test: Apply the test sample (0.02 g / cm²) to the back skin and leave it on for 48 hours. Observe for reactions such as erythema and edema. Use continuously for 28 days, and assess irritation symptoms daily (according to ICDRG scoring criteria). The final statistics showed that the stimulation index of all samples was ≤0.3 (no stimulation), which meets the ISO 10993-10 standard.
[0064] Experiment 4: The effect of hair serum on hair resilience Test samples: hair essence 1-3, ①-⑪, blank hair essence.
[0065] Volunteers aged 35-50 were randomly divided into 15 groups of 10 volunteers each, with 5 males and 5 females. All 15 groups shampooed their hair every two days using the same shampoo and dosage. Each group applied the corresponding test sample, left it evenly for 1.5 minutes, rinsed for 1 minute, and then dried. During the first shampoo, detached hair strands were collected, and their tensile strength A0 was tested. After the 30th shampoo, after applying hair care products and drying, detached hair strands were collected, and their tensile strength A30 was tested. The rate of change in tensile strength δ / % was calculated as follows: δ / % = 100% × (A30 - A0) / A0.
[0066] The tensile strength test method is as follows: Select hair strands and use the Fibra.one multi-functional hair tester to test the tensile strength of 10 hair strands. Record the tensile strength values and take the average value. The higher the measured value, the better the tensile strength.
[0067] Table 3. Effects of hair serums on hair resilience. Group A0 / gf A30 / gf δ / % Hair serum 1 97.41 121.58 24.81 Hair serum 2 98.36 119.37 21.36 Hair serum 3 97.05 119.31 22.94 Hair serum ① 97.21 107.27 10.35 Hair care essence ② 97.44 114.43 17.44 Hair serum ③ 98.01 114.74 17.07 Hair care essence ④ 98.26 114.32 16.34 Hair care essence ⑤ 98.35 113.32 15.22 Hair care essence ⑥ 97.42 113.06 16.05 Hair serum ⑦ 97.09 113.95 17.37 Hair serum ⑧ 97.31 106.48 9.42 Hair care essence 9 98.52 109.39 11.03 Hair serum 10 98.33 108.35 10.19 Hair care essence ⑪ 96.54 111.43 15.42 Blank Hair Essence 97.35 99.53 2.24 As shown in Table 3, hair care essences 1-3 and ①-⑪ all have a certain effect on improving hair strength compared to the blank hair care essence; comparing the results of hair care essence 1 with hair care essences ①-②, it can be seen that the plant extracts and complex peptides in the composition of the present invention have a significant synergistic effect; comparing the results of hair care essence 1 with hair care essences ③-⑥, it can be seen that the plant extracts of the present invention, including Panax notoginseng root extract, Cnidium monnieri extract, and coral algae extract, have a significant synergistic effect with the complex peptides, and their combined use can significantly improve the tensile strength of hair; comparing the results of hair care essence 1 with hair care essence ⑦, it can be seen that Panax notoginseng root extract... Within the mass ratio range defined in this invention, the extracts of *Cnidium monnieri*, *Cnidium monnieri*, and *Coral algae* exhibit the best efficacy in enhancing hair's tensile strength. A comparison of hair care essence 1 and hair care essences ⑧-⑩ shows that the tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 in the compound polypeptide of this invention have a significant synergistic effect with the plant extracts, and their combined use can significantly enhance hair's tensile strength. A comparison of hair care essence 1 and hair care essence ⑪ shows that within the mass ratio range defined in this invention, the tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3 exhibit the strongest synergistic effect with the plant extracts, resulting in the best efficacy in enhancing hair's tensile strength.
[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.
Claims
1. A composition having the effect of preventing hair breakage, characterized in that, The composition comprises the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and Coral algae extract, with a mass ratio of 1:(3-5):(1-3); the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:(0.5-0.7):(0.2-0.4); the mass ratio of the plant extracts to the complex polypeptides is 1:(0.01-0.5).
2. The composition according to claim 1, characterized in that, The composition comprises the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and Coral algae extract, with a mass ratio of 1:(3.5-4.5):(1.5-2.5); the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:(0.6-0.7):(0.2-0.3); the mass ratio of the plant extracts to the complex polypeptides is 1:(0.05-0.1).
3. The composition according to claim 1, characterized in that, The composition comprises the following raw materials: plant extracts and complex polypeptides; the plant extracts include Panax notoginseng root extract, Cnidium monnieri fruit extract, and coral algae extract, with a mass ratio of 1:4:2; the complex polypeptides include tripeptide-2, acetyl tetrapeptide-3, and pentapeptide-3, with a mass ratio of 1:0.6:0.3; and the mass ratio of the plant extracts to the complex polypeptides is 1:0.
1.
4. The use of a composition having anti-breakage effect as described in any one of claims 1-3 in the preparation of shampoo and hair care products having anti-breakage effect.
5. The application according to claim 4, characterized in that, The hair care products include any one of shampoo, conditioner, hair serum, and hair mask.
6. A hair care essence with anti-breakage effect, characterized in that, The hair care essence includes the composition with anti-breakage effect as described in any one of claims 1-3.
7. The hair care essence according to claim 6, characterized in that, The hair care essence also includes oils, polyols, deionized water, pH adjusters, thickeners, preservatives, chelating agents, and fragrances.
8. The hair care essence according to claim 6, characterized in that, The oils include at least one of jojoba seed oil, grape seed oil, meadowfoam seed oil, babassu seed oil, macadamia seed oil, tea seed oil, and plantain seed oil.
9. The hair care essence according to claim 6, characterized in that, The polyol includes at least one of glycerol, 1,2-hexanediol, caprylyl glycol, ethylhexylglycerin, propylene glycol, glyceryl glucoside, pentaerythritol, and inositol; the preservative includes at least one of sodium benzoate, methylparaben, and potassium sorbate; the thickener includes at least one of hydroxyethyl cellulose, xanthan gum, hydroxypropyl methylcellulose, and carbomer; the chelating agent includes at least one of disodium EDTA, sodium tripolyphosphate, and capryloyl hydroxyvalerate; and the pH adjuster includes at least one of citric acid and lactic acid.
10. The hair care essence according to claim 6, characterized in that, The preparation method of the hair care essence includes the following steps: S1: Heat polyol, chelating agent, composition with anti-hair breakage effect, and deionized water to 60-70℃, stir evenly, and keep warm for 60-80 minutes to obtain phase A; S2: Mix the oil and fat evenly to obtain phase B; S3: After phase A cools down to 40-50℃, add it to phase B and stir evenly to obtain mixed phase C; S4: After the mixed phase C is cooled to 30-35℃, add the thickener, preservative and fragrance to the mixed phase C, stir evenly to obtain the mixed phase D; S5: Use a pH adjuster to adjust the pH of the mixed phase D to 5.5-6.5, stir evenly, and let stand for 30-60 minutes; then discharge.