Nutrient composition for promoting hair growth and preparation method thereof

Abstract

The invention relates to the field of hair growth nutrients, and discloses a nutrient composition for promoting hair growth and a preparation method thereof, the composition comprises a basic hair growth nutrient group and an absorption-promoting synergistic group; the basic hair growth nutrition group comprises the following components in parts by weight: 0.3-0.8 part of ferrous lactate, 2-5 parts of zinc gluconate, 5-8 parts of magnesium stearate, 3-8 parts of a coating material and 17-25 parts of milk mineral salt; the absorption-promoting synergistic group comprises the following components in parts by weight: 0.2-1.5 parts of a black pepper extract, 2-10 parts of lecithin and 2-8 parts of casein phosphopeptides, the black pepper extract and the lecithin are firstly released, an intestinal epithelium channel is opened by utilizing a biological penetration promoting effect, and a pre-permeation state is established; the subsequently released CPP is complexed with divalent metal ions in intestinal juice to prevent precipitation, so that the CPP is synchronously and rapidly absorbed through a pre-opened channel, and the problems of competitive antagonism and difficult absorption of high-dose minerals are effectively solved.

Description

Technical Field

[0001] This invention relates to the field of hair growth nutrients, specifically to a nutrient composition that promotes hair growth and its preparation method. Background Technology

[0002] With the fast pace of modern life and increasing work pressure, hair loss and damaged hair have become common health concerns. Hair growth is a highly metabolic process that relies heavily on a sufficient and balanced supply of nutrients. Supplementing with protein, vitamins, and key minerals such as iron, zinc, and calcium orally is currently the mainstream method for improving the scalp microenvironment and promoting hair follicle regeneration. Most commercially available hair growth nutritional compositions attempt to meet hair growth needs by piling on multiple nutrients, typically using simple physical mixing processes.

[0003] However, existing simple compound products often fail to achieve the expected hair regrowth effect in practical applications, and their core technological bottlenecks are mainly concentrated in the following three aspects:

[0004] First, there is a contradiction between the chemical stability and solubility of minerals. After passing through the stomach and into the intestines, polyvalent metal ions such as iron, zinc, and calcium face a rapid pH change from acidic to slightly alkaline. Without specific protective mechanisms, these free metal ions readily hydrolyze in intestinal fluid or react chemically with coexisting phosphate and phytate ions, forming insoluble precipitates. This not only causes the active ingredients to be lost due to solidification before absorption but may also trigger gastrointestinal discomfort.

[0005] Second, there is the competitive antagonistic effect of ion absorption. In the traditional mixed-release mode, multiple divalent metal ions simultaneously reach the surface of intestinal epithelial cells. Because the human body has non-specificity to transporters of divalent metal ions (such as DMT1), a high concentration of a particular ion (such as calcium) will competitively occupy the transport channel, significantly inhibiting the uptake of other trace elements (such as iron and zinc). This antagonistic effect means that simply increasing the dosage does not translate into effective concentrations in the blood.

[0006] Third, the physical barrier of the intestinal epithelium limits absorption. The surface of intestinal absorptive epithelial cells is covered by a dense lipid bilayer, forming a natural hydrophobic barrier. Existing formulations lack effective bio-permeability enhancement strategies, resulting in a large number of highly hydrophilic bioactive peptides and water-soluble vitamins being unable to effectively penetrate this lipid barrier. Furthermore, due to the lack of mechanisms to actively open intercellular spaces or activate absorption channels, most nutrients can only rely on passive diffusion, resulting in extremely low bioavailability and making it difficult to accumulate in high concentrations at target sites such as scalp hair follicles, which are far from the administration site, via blood circulation. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a nutrient composition for promoting hair growth and its preparation method, which solves the problems of low absorption rate of minerals due to chemical precipitation and competitive antagonism in existing hair growth formulas, and poor bioavailability of hydrophilic nutrients due to difficulty in breaking through the intestinal lipid barrier.

[0008] To achieve the above objectives, the first aspect of the present invention provides a nutrient composition for promoting hair growth, comprising a basic hair growth nutrient group and an absorption-enhancing synergistic group.

[0009] The components and weight percentages of the basic hair growth nutrient group include: 7-10 parts vitamin C, 0.3-0.8 parts ferrous lactate, 4-8 parts compound vitamin B powder, 2-5 parts zinc gluconate, 2-5 parts grape seed extract, 12-18 parts γ-aminobutyric acid, 5-8 parts magnesium stearate, 3-8 parts coating material, and 17-25 parts milk mineral salts.

[0010] The components and weight proportions of the absorption-promoting synergistic group include: 0.2-1.5 parts of black pepper extract, 2-10 parts of lecithin, and 2-8 parts of casein phosphopeptide.

[0011] Preferably, the black pepper extract contains ≥95% piperine; the lecithin is selected from soybean lecithin or egg yolk lecithin, wherein the acetone-insoluble content is ≥95%; and the total nitrogen content of the casein phosphopeptide is 10%-15%.

[0012] Preferably, the content of proanthocyanidins in the grape seed extract is ≥95%.

[0013] Preferably, the purity of the γ-aminobutyric acid is ≥95%; and the calcium content of the milk mineral salt is ≥25%.

[0014] A second aspect of the present invention provides a method for preparing a nutrient composition that promotes hair growth, comprising the following steps:

[0015] Step S1, Raw material grouping: Black pepper extract and lecithin are separated and used as synergistic components; coating material and magnesium stearate are separated and used as base powder; the remaining raw materials are used as base powder.

[0016] Step S2, Crushing and Sieving: The base powder is crushed, sieved and mixed evenly to obtain premixed powder;

[0017] Step S3: Preparation of permeation-enhancing emulsifying adhesive: Dissolve and disperse black pepper extract and lecithin in an ethanol solution, and stir evenly to obtain permeation-enhancing emulsifying adhesive;

[0018] Step S4, Granulation and Drying: The penetration-enhancing emulsified adhesive is sprayed into the premixed powder as a wetting agent to form a soft material and granulate it, followed by drying.

[0019] Step S5, Granulation: Granulate the dried granules;

[0020] Step S6, Mixing: Mix the granulated particles with the magnesium stearate taken out in step S1;

[0021] Step S7, tableting: The mixed materials are compressed into tablets to obtain plain tablets;

[0022] Step S8, Coating: The coating material taken out in step S1 is prepared into a coating solution, and the tablets after compression are coated to obtain a nutrient composition.

[0023] Preferably, the sieve mesh size is 80-100 mesh.

[0024] Preferably, the concentration of the ethanol solution is 30%-40%, and the black pepper extract and lecithin form a uniform emulsion dispersion system in the ethanol.

[0025] Preferably, the environmental conditions for the drying process are controlled as follows: temperature 50℃-60℃, humidity 2%-4%; in step S5, the mesh size of the granulated particles is controlled at 16-18 mesh.

[0026] Preferably, the tableting is performed at a temperature of 18℃-24℃ and a relative humidity of 45%-55%, and the weight difference of the tablets after tableting is controlled within ±3%.

[0027] Preferably, in step S8, the coating process is carried out under the conditions of an inlet air temperature of 70℃-80℃, an outlet air temperature of 40℃-55℃, and a coating pan rotation speed of 3-5 revolutions / minute, and the coating weight gain is controlled within ±3%.

[0028] This invention provides a nutrient composition for promoting hair growth and a method for preparing the same. It has the following beneficial effects:

[0029] This invention enriches black pepper extract and lecithin on the surface of a composition. After the user takes it, the two are released first. The lipophilic modification of lecithin and the bio-permeability enhancement of piperine improve the intestinal microenvironment and open the absorption channels of epithelial cells, establishing a pre-permeable state. Subsequently, the released casein phosphopeptides rapidly capture divalent metal ions such as calcium, iron, and zinc in the weakly alkaline intestinal fluid, generating highly stable soluble complexes, which can be rapidly absorbed by the human body through the pre-opened channels. This solves the problem that high doses of compound minerals in hair growth formulas are difficult to absorb simultaneously due to competition, antagonism, and precipitation. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the preparation process of the present invention;

[0031] Figure 2 This is a schematic diagram showing the results of the apparent permeability coefficient determination of the key components in each experimental group in Test Example 1 of the present invention;

[0032] Figure 3 This is a statistical diagram of the hair growth index on the backs of mice in each experimental group in Test Example 2 of the present invention. Detailed Implementation

[0033] Now combined with the appendix Figure 1 -Appendix Figure 3 The present invention will be further described in detail below.

[0034] The main raw materials and reagents used in the following examples and comparative examples have the following sources and specifications. Reagents not specifically mentioned are all commercially available analytical grade or higher grade products.

[0035] Black pepper extract is a commercially available standardized plant extract. It appears as a pale yellow fine powder, with a piperine content of ≥95% and CAS number 94-62-2.

[0036] Soy lecithin is a commercially available food-grade soybean extract with an acetone-insoluble content of ≥95% and a hexane-insoluble content of ≤0.3%. Its CAS number is 8002-43-5.

[0037] Egg yolk lecithin: High-purity pharmaceutical grade, phosphatidylcholine (PC) content ≥80%, iodine value 60-70gI2 / 100g, peroxide value ≤3.0meq / kg;

[0038] Casein phosphopeptide (CPP) is a commercially available food-grade dairy derivative with a total nitrogen content ≥10%, peptide content ≥85%, calcium binding capacity ≥6000mg / 100g, and CAS number 691364-49-5.

[0039] The coating material is a premix of hydroxypropyl methylcellulose (HPMC) and polyethylene glycol (PEG), wherein HPMC has a viscosity of 5-15 mPa·s and is used for gastric-soluble film coating.

[0040] Microcrystalline cellulose: pharmaceutical excipient grade, model PH101, average particle size approximately 50μm, loss on drying ≤5.0%.

[0041] Example 1: This example provides a method for preparing a nutrient composition that promotes hair growth.

[0042] 1. Formula composition:

[0043] Basic hair growth nutrient group (by weight): Vitamin C 8 parts, ferrous lactate 0.5 parts, compound vitamin B powder 6 parts, zinc gluconate 3 parts, grape seed extract 4 parts, γ-aminobutyric acid 17 parts, magnesium stearate 6 parts, coating material 5 parts, milk mineral salts 21 parts.

[0044] Synergistic effect of absorption promotion group (parts by weight): 1.2 parts of black pepper extract (piperine content 95%), 6 parts of lecithin (soy lecithin), and 5 parts of casein phosphopeptide (total nitrogen content 12%).

[0045] 2. Preparation method:

[0046] Step 1, Raw Material Pretreatment: Accurately weigh each component. Separate the black pepper extract and lecithin as outer functional components for later use; separate the coating material and magnesium stearate for later use; and reserve the remaining solid raw materials, including milk mineral salts, vitamins, and peptides, as base powders for later use.

[0047] Step 2, Premixing: Sift the base powder and grind it to 90 mesh, then place it in a multi-directional motion mixer and mix it evenly to obtain premixed powder;

[0048] Step 3: Preparation of synergistic binder: Dissolve and disperse the black pepper extract and lecithin reserved in Step 1 in a 35% concentration of food-grade ethanol aqueous solution, and stir at high speed to prepare a uniform emulsified binder.

[0049] Step 4, Granulation and Drying: Using a wet granulation process, the emulsified binder is evenly sprayed into the premixed powder in the form of atomization to form a soft material and then granulated, so that the black pepper extract and lecithin are enriched on the surface of the particles; the obtained particles are dried in an environment with a drying temperature of 55°C and a moisture content controlled at 3%.

[0050] Step 5, Granulation: Granulate the dried granules, controlling the sieve mesh size to 17 mesh;

[0051] Step 6, General Mixing: Place the granulated particles and the magnesium stearate taken in Step 1 into a three-dimensional mixer and mix for 24 minutes;

[0052] Step 7, tableting: The mixed materials are tableted. The tableting temperature is controlled at 20℃, the relative humidity is 50%, and the tablet weight difference is controlled within ±3% to obtain unmixed tablets.

[0053] Step 8, Coating: Prepare a coating solution from the coating material taken in Step 1, and coat the tablets after compression. The inlet air temperature for coating is 75℃, the outlet air temperature is 50℃, the coating pan speed is 4 revolutions / minute, and the coating weight gain is controlled at 2%. Finally, the nutrient composition is obtained.

[0054] Example 2: This example provides a nutrient composition that promotes hair growth and its preparation method.

[0055] 1. Formula composition:

[0056] Basic hair growth nutrient group (by weight): Vitamin C 7 parts, ferrous lactate 0.3 parts, compound vitamin B powder 4 parts, zinc gluconate 2 parts, grape seed extract 2 parts, γ-aminobutyric acid 12 parts, magnesium stearate 5 parts, coating material 3 parts, milk mineral salts 17 parts.

[0057] Synergistic effect of absorption enhancement group (parts by weight): 0.2 parts black pepper extract, 2 parts lecithin (soy lecithin), 2 parts casein phosphopeptide;

[0058] 2. Preparation method:

[0059] Steps 1 to 2: Same as in Example 1, except that the base powder is pulverized to 80 mesh;

[0060] Step 3: Preparation of synergistic binder: Dissolve and disperse black pepper extract and lecithin in a 30% concentration of food-grade ethanol aqueous solution to prepare an emulsified binder;

[0061] Step 4, Granulation and Drying: Spray the emulsified binder into the premixed powder to granulate; set the drying temperature to 50℃ and control the particle moisture content at 2%;

[0062] Step 5, Granulation: The mesh size of the granules is controlled at 16 mesh;

[0063] Step 6, General Mixing: Mixing time is 20 minutes;

[0064] Step 7, tableting: The tableting environment temperature is 18℃, the relative humidity is 45%, and the tablet weight difference is controlled within ±3% to obtain unprocessed tablets;

[0065] Step 8, Coating: The inlet air temperature for coating is 70℃, the outlet air temperature is 40℃, the coating pan rotates at 3 revolutions per minute, the weight gain is 2%, and the final nutrient composition is obtained.

[0066] Example 3: This example provides a nutrient composition that promotes hair growth and its preparation method.

[0067] 1. Formula composition:

[0068] Basic hair growth nutrient group (by weight): Vitamin C 10 parts, ferrous lactate 0.8 parts, compound vitamin B powder 8 parts, zinc gluconate 5 parts, grape seed extract 5 parts, γ-aminobutyric acid 18 parts, magnesium stearate 8 parts, coating material 8 parts, milk mineral salt 25 parts.

[0069] Synergistic effect of absorption enhancement group (parts by weight): 1.5 parts black pepper extract, 10 parts lecithin (egg yolk lecithin), 8 parts casein phosphopeptide;

[0070] 2. Preparation method:

[0071] Steps 1 to 2: Same as in Example 1, except that the base powder is pulverized to 100 mesh;

[0072] Step 3: Preparation of synergistic binder: Dissolve and disperse black pepper extract and lecithin in a 40% concentration of food-grade ethanol aqueous solution, and stir evenly to prepare an emulsified binder;

[0073] Step 4, Granulation and Drying: Spray the emulsified binder into the premixed powder to granulate; set the drying temperature to 60℃ and control the particle moisture content at 4%;

[0074] Step 5, Granulation: The mesh size of the granules is controlled at 18 mesh;

[0075] Step 6, General Mixing: Mixing time is 30 minutes;

[0076] Step 7, tableting: The tableting environment temperature is 24℃, the relative humidity is 55%, and the tablet weight difference is controlled within ±3% to obtain unprocessed tablets;

[0077] Step 8, Coating: The inlet air temperature for coating is 80℃, the outlet air temperature is 55℃, the coating pan speed is 5 revolutions / minute, the weight gain is 2%, and the final nutrient composition is obtained.

[0078] Comparative Example 1:

[0079] Compared with Example 1, the differences are: no absorption-enhancing synergists are added (i.e., no black pepper extract, lecithin, and casein phosphopeptide are added); the missing weight parts are made up by microcrystalline cellulose; in step 3 of the preparation, a 35% concentration of food-grade ethanol solution is directly used as a binder; and the other raw materials and preparation steps are the same.

[0080] Comparative Example 2:

[0081] Compared with Example 1, the difference is that only the black pepper extract and lecithin in the absorption-promoting synergistic group are removed, while casein phosphopeptide (CPP) is retained; the missing weight parts are made up by microcrystalline cellulose; in step 3 of the preparation, a 35% concentration of food-grade ethanol solution is directly used as a binder; and the other raw materials and preparation steps are the same.

[0082] Comparative Example 3:

[0083] Compared with Example 1, the difference is that only casein phosphopeptide (CPP) in the absorption-promoting synergistic group is removed, while black pepper extract and lecithin are retained; the missing weight fractions are made up by microcrystalline cellulose, the original step position of casein phosphopeptide remains unchanged (i.e., it does not participate), and the other raw materials and preparation steps are the same.

[0084] Comparative Example 4:

[0085] Compared to Example 1, the difference lies in the order of raw material addition and the granulation method. Specifically:

[0086] In step 1, black pepper extract and lecithin are not separated, but are directly used together with other solid ingredients of the basic hair growth nutrient group as a base powder.

[0087] In step 3, instead of preparing an emulsified adhesive, a 35% concentration of food-grade ethanol solution is used directly as a common adhesive.

[0088] In step 4, a common binder is sprayed into a mixture of black pepper and lecithin powder for granulation. The rest is the same.

[0089] Comparative Example 5:

[0090] Compared with Example 1, the difference is that the lecithin in the absorption-promoting synergistic group is replaced with an equal part by weight of hydroxypropyl methylcellulose (HPMC); in step 3, black pepper extract and HPMC are dissolved and dispersed in an ethanol solution to prepare a colloidal binder (non-lipid emulsion system), and the remaining raw materials and preparation steps are the same.

[0091] Test Example 1:

[0092] 1. Experimental objective:

[0093] Using a human colon adenocarcinoma cell (Caco-2) monolayer model to simulate the human small intestinal epithelial cell environment, the apparent permeability coefficients (Papp) of key nutrients (marked by ferrous ions, calcium ions, and anthocyanins) in the compositions prepared in Examples 1-3 and Comparative Examples 1-5 were detected to evaluate the intestinal absorption efficiency and bioavailability of the compositions.

[0094] 2. Experimental Methods:

[0095] Cell culture and modeling: Caco-2 cells were seeded onto the polycarbonate membrane of a Transwell chamber at a density of 1 × 10⁻⁶ cells / mL. 5 pcs / cm 2 Incubate in a CO2 incubator for 21 days, changing the medium every other day, until the transmembrane resistivity (TEER) value is greater than 500 Ω·cm. 2 The construction of the dense monolayer cell model has been confirmed.

[0096] Preparation of test solutions: The tablets prepared in Examples 1-3 and Comparative Examples 1-5 were ground into powder and dispersed in HBSS (Black's Balanced Salt Solution, pH 7.4) buffer. Insoluble excipient precipitates were removed by centrifugation, and the concentrations of key components in each test solution were adjusted to be consistent, thus serving as the supply solutions.

[0097] Transport experiment:

[0098] Remove the original culture medium from the wells of the culture plate and wash the cells three times with preheated HBSS.

[0099] Add 0.5 mL of each test solution to the supply side (top, AP side) of the Transwell chamber, and add 1.5 mL of blank HBSS solution to the receiving side (base, BL side). Incubate in a 37°C constant temperature shaker. At 30 min, 60 min, 90 min, and 120 min, respectively, aspirate the sample solution from the receiving side and immediately add an isothermal volume of blank HBSS.

[0100] Detection and analysis:

[0101] Determination of ferrous ions (Fe2+) in the receiving solution using atomic absorption spectrometry (AAS) 2+ ) and calcium ions (Ca 2+ The concentration of ).

[0102] The concentration of anthocyanins in the receiving solution was determined using high-performance liquid chromatography (HPLC).

[0103] Data processing: The apparent permeability coefficient was calculated using the formula Papp = (dQ / dt) / (A × C0). Where dQ / dt is the drug transport rate per unit time (μg / s), and A is the membrane area (cm²). 2 C0 represents the initial concentration on the supply side (μg / mL).

[0104] 3. Experimental Data

[0105] Table 1: Results of Apparent Permeability Coefficient Measurement of Key Components in Each Experimental Group

[0106] Grouping <![CDATA[Ferrous ion Papp (×10 -6 cm / s)]]> <![CDATA[Calcium ion Papp (×10 -6 cm / s)]]> <![CDATA[Anthocyanin Papp (×10 -6 cm / s)]]> Example 1 8.42±0.35 13.15±0.62 4.87±0.28 Example 2 7.96±0.41 12.08±0.55 4.35±0.31 Example 3 8.61±0.29 13.44±0.48 5.02±0.24 Comparative Example 1 1.83±0.15 3.52±0.22 0.64±0.08 Comparative Example 2 4.12±0.26 9.87±0.43 0.95±0.11 Comparative Example 3 2.05±0.19 3.96±0.31 3.78±0.25 Comparative Example 4 5.86±0.33 10.22±0.51 2.94±0.18 Comparative Example 5 6.13±0.28 10.45±0.46 1.82±0.14

[0107] 4. Conclusion Analysis

[0108] According to Table 1 and Figure 2 It can be seen that the apparent permeability coefficients of various nutrients in Examples 1-3 are significantly higher than those in the comparative examples, indicating that there is a clear interaction between the basic hair growth nutrient group and the absorption-promoting synergistic group, which improves the transmembrane efficiency of active ingredients.

[0109] Analysis of the absorption data for metal ions showed that the transport efficiency of ferrous and calcium ions in the example group remained at a high level. In contrast, in control ratio 3 (lacking casein phosphopeptide CPP), the Papp values ​​of calcium and ferrous ions decreased significantly (3.96 × 10⁻⁶, respectively). -6 cm / s and 2.05×10 -6 (cm / s), close to the blank level of Comparative Example 1. This confirms the stabilizing effect of CPP on divalent metal ions in the weakly alkaline environment of the small intestine. By preventing the formation of insoluble phosphate precipitates, it maintains the dissolved state and free concentration of substances at the absorption interface. Otherwise, simply adding a permeation enhancer cannot solve the problem of absorption source loss caused by mineral precipitation.

[0110] Regarding the absorption data of proanthocyanidins, a high-molecular-weight polyphenol, the Papp value of proanthocyanidins in Comparative Example 2 (lacking black pepper extract and lecithin) was only 0.95 × 10⁻⁶. -6 cm / s, much lower than 4.87 × 10 cm / s in Example 1. -6 cm / s. This indicates that piperine in black pepper extract overcomes the limitation of large plant polyphenol molecules in penetrating the dual barriers of aqueous mucus layer and cell membrane by regulating the lipid fluidity of epithelial cell membranes and inhibiting the P-glycoprotein efflux pump, in conjunction with the emulsified micelle carrier system constructed from lecithin.

[0111] Furthermore, the influence of process parameters on absorption performance is clearly evident in the comparison between Example 1 and Comparative Example 4. Comparative Example 4 uses a common mixing process, and the Papp values ​​of its three components are all lower than those of Example 1. At the physical mechanism level, the specific granulation process used in Example 1 enriches the emulsion binder containing black pepper extract and lecithin on the outer layer of the particles. When the tablet disintegrates, the mucosal absorption-promoting components are preferentially released and form a high-concentration absorption microenvironment on the surface of local intestinal epithelial cells. Subsequently, the released core nutrients can pass through this pre-treated absorption channel rapidly. The uniform distribution in Comparative Example 4 results in insufficient concentration of the absorption-promoting agent at the local interface, failing to fully open the tight junctions between cells or saturate the efflux pumps.

[0112] Furthermore, in Comparative Example 5, the Papp value of anthocyanins decreased significantly after HPMC was used to replace lecithin. This indicates that lecithin is not merely a physical binder; its phospholipid bimolecular structure acts as an amphiphilic surfactant. In an ethanol-water system, it forms a liposome-like structure that encapsulates poorly soluble drugs. This structure exhibits higher biocompatibility with cell membranes, facilitating entry into cells via membrane fusion or pinocytosis. In contrast, general high molecular weight cellulose (HPMC) lacks such biomembrane affinity mechanisms.

[0113] Test Example 2:

[0114] 1. Experimental steps:

[0115] Six-week-old male C57BL / 6 mice, weighing 20±2g, were selected and placed in an SPF-grade animal room for 7 days of acclimatization feeding, during which they had free access to food and water. Because this strain of mice has good synchronicity of hair follicle cycles on the back and black fur, it is easy to observe hair growth and skin color changes (from pink to gray-black, indicating the start of the growth phase).

[0116] The back hair of mice was removed using an electronic hair trimmer, covering an area of ​​approximately 2cm × 3cm. A mixture of rosin and paraffin (1:1) was then applied, cooled, and peeled off. This mechanical stimulation induced the hair follicles on the back to simultaneously enter the resting phase. Individuals with red or broken skin were discarded, and mice with pink skin and no remaining hair were selected. The mice were then randomly divided into 9 groups of 10 mice each: a blank control group (administered an equal volume of saline), a positive control group (administered finasteride suspension, equivalent dose of 5mg / kg / day), Examples 1-3, and Comparative Examples 1-5. All groups were administered the medication by gavage, with the equivalent dose calculated based on the conversion factor between mouse and human surface areas. Administration was once daily for 30 consecutive days.

[0117] During the experiment, the color change of the back skin and the hair budding of the mice were observed daily. After 30 days of drug administration, the mice were sacrificed, and the newly grown hair in the drug administration area on the back was cut off and weighed. The back skin tissue was excised, fixed with 4% paraformaldehyde, embedded in paraffin, sectioned and stained with hematoxylin and eosin (HE), and the morphology of hair follicles was observed under a microscope and the number of hair follicles per unit field of view was calculated.

[0118] 2. Experimental Data

[0119] Table 2: Statistics on hair growth indicators on the backs of mice in each experimental group

[0120] Grouping Length of new hair growth (mm) <![CDATA[Hair follicle density (number / mm 2 )]]> Initial hair regrowth time (days) Example 1 9.42±0.83 36.15±2.62 9.2±1.1 Example 2 8.85±0.91 33.08±3.41 10.4±1.3 Example 3 9.68±0.77 37.44±2.18 8.8±0.9 Comparative Example 1 5.21±0.65 18.52±2.05 16.5±2.1 Comparative Example 2 6.88±0.72 24.87±2.84 13.2±1.8 Comparative Example 3 6.13±0.84 22.96±3.12 14.1±1.5 Comparative Example 4 7.94±0.79 29.22±2.55 11.5±1.6 Comparative Example 5 7.55±0.68 28.45±2.44 12.1±1.2 Positive control group 9.95±0.62 38.28±2.21 8.2±0.8

[0121] 3. Conclusion Analysis

[0122] According to Table 2 and Figure 3 It can be seen that the mice treated in Examples 1-3 showed better results than the blank control group and the comparative groups in terms of new hair length, hair follicle density, and hair regrowth initiation time. The hair follicle density in Example 1 reached 36.15 follicles / mm². 2 Compared with the positive drug finasteride group (38.28 / mm), 2 The levels are comparable, indicating that the nutrient composition of the present invention can produce clear pharmacological activity to promote hair growth after oral ingestion.

[0123] The data differences reflect the direct impact of the preparation process on the efficacy. Comparing Example 1 and Comparative Example 4 (ordinary mixing process), although the composition of the formulations is completely identical, the hair follicle density of Comparative Example 4 is 29.22 follicles / mm². 2The concentration was significantly lower than in Example 1. Combined with the conclusions of Example 1, this confirms that the conventional mixing process failed to effectively improve the intestinal absorption rate of nutrients, resulting in insufficient concentrations of effective ingredients entering the bloodstream and ultimately reaching the dermis, thus failing to maximize the activation of hair follicle cells. The sequential release structure ensures the in vivo utilization rate of trace elements and core active ingredients, thereby macroscopically manifesting as a superior hair growth effect.

[0124] The absence of certain components negatively impacted the final efficacy. Data from Comparative Example 2 (without penetration enhancer) and Comparative Example 3 (without anti-precipitation group) were significantly lower than those from the Example group, with Comparative Example 3 showing a hair length of only 6.13 mm. This indicates that the lack of CPP's stabilizing effect on minerals such as calcium and iron, or the lack of the black pepper / lecithin system's delivery of macromolecular plant extracts, leads to nutrient loss or metabolic depletion in the digestive tract, failing to meet the specific coenzyme factors required for hair protein synthesis and hair follicle cell division. Providing only a nutrient source without addressing the transport bottleneck (as in Comparative Example 2) limits its biological effects.

[0125] The data for Comparative Example 5 (HPMC replacing lecithin) fell between those of Comparative Example 4 and Example 1, indicating that while HPMC possesses certain adhesive and sustained-release functions, its ability to promote transmembrane transport of intestinal epithelial cells is weaker than that of lecithin. Furthermore, lecithin, as an endogenous phospholipid component, not only acts as a carrier but also participates in cell membrane repair and signal transduction.

[0126] Furthermore, in an expanded embodiment, in addition to the basic formula described above, the present invention can also add additional nutritional components to enhance efficacy; the specific amount added can be determined according to the actual product positioning. Specifically, based on specific embodiment 1, the following can also be added:

[0127] Mushroom powder contains abundant vitamin D (especially vitamin D2) and fungal polysaccharides. Vitamin D receptors play a key role in hair follicle biology, inducing hair follicle stem cell differentiation and regulating the hair growth cycle, thus promoting hair growth.

[0128] Grape powder contains highly active proanthocyanidins (OPC) and resveratrol, which, as powerful antioxidants, can eliminate free radicals on the scalp, protect hair follicle cells from oxidative stress damage, improve scalp microcirculation, and also promote hair growth.

[0129] Fish collagen peptides are rich in hydroxyproline and glycine, and have a small molecular weight that makes them easily absorbed. These amino acids are important precursors for the synthesis of hair keratin, which can increase hair diameter and toughness, repair the connective tissue around hair follicles, and promote hair growth.

[0130] Oyster peptides contain high concentrations of bio-organic zinc and taurine. Zinc is an activator of various metalloenzymes in the body and directly participates in the synthesis of hair keratin and cell division. Taurine has also been proven to prevent hair follicle fibrosis and promote hair growth.

[0131] Donkey-hide gelatin peptides contain abundant hematopoietic factors and small molecule collagen. According to the theory of traditional Chinese medicine that hair is the surplus of blood, donkey-hide gelatin peptides can nourish blood, improve the oxygen carrying capacity and nutrient delivery efficiency of blood in the scalp, and also promote hair growth.

[0132] Sea cucumber peptides contain abundant arginine and acidic mucopolysaccharides. Arginine is not only a raw material for protein synthesis, but it can also promote the production of nitric oxide, dilate scalp blood vessels, improve the nutritional supply to hair follicles, and promote hair growth.

[0133] Sorbitol has excellent moisturizing and penetration-regulating effects. It can regulate the hydration status of scalp cells, maintain the moist environment of hair follicles and surrounding skin, prevent hair loss caused by dry scalp, and also promote the optimization of the hair growth environment.

[0134] Maca powder, rich in unique bioactive substances such as macaene and macamides, has significant endocrine regulation and anti-fatigue effects. It can alleviate the prolonged resting phase of hair follicles caused by mental stress or hormonal fluctuations, while also improving the body's energy metabolism level, providing power support for the rapid division and proliferation of hair matrix cells, and also promoting hair growth.

[0135] Furthermore, ferrous gluconate can be used to provide divalent ferrous ions to achieve the iron-supplementing and hematopoietic effects of ferrous lactate. Since ferrous gluconate contains organic iron with high bioavailability and low gastrointestinal irritation, it can effectively participate in the synthesis of hemoglobin, enhance the oxygen-carrying capacity of blood, ensure that hair follicles receive sufficient oxygen supply, and prevent diffuse hair loss caused by iron deficiency anemia.

[0136] B-enriched yeast powder can also be used to provide B-complex vitamin nutrition to play a role in regulating the metabolism of B-complex vitamin powder. Because B-enriched yeast powder contains highly active natural B vitamins and yeast protein that have been transformed through biological fermentation, it can not only regulate scalp sebum secretion and improve the environment of seborrheic dermatitis, but also synergistically promote protein metabolism and provide necessary coenzyme support for the synthesis of hair keratin.

[0137] Zinc-enriched yeast powder can also be used to provide organic zinc to achieve the oil-controlling and hair-strengthening effect of zinc gluconate. Because zinc-enriched yeast powder contains bioavailable zinc that is bound to yeast protein and amino acids, it has excellent intestinal affinity and absorption rate, and can efficiently enter hair follicle cells to participate in the catalytic process of metalloenzymes, inhibit 5α-reductase activity and promote keratin cross-linking, thereby enhancing the toughness of hair roots.

[0138] Finally, turmeric powder can be used to provide a powerful antioxidant to achieve the anti-aging and anti-inflammatory effects of grape seed extract. Since turmeric powder contains a high concentration of curcumin, which is a polyphenol, its anti-inflammatory and free radical scavenging abilities can be utilized to reduce the micro-inflammatory response around the hair follicles, resist hair follicle cell apoptosis caused by oxidative stress, and thus maintain the growth activity of hair follicles.

[0139] In addition, other nutritional components may be added, including but not limited to:

[0140] Saw palmetto extract contains phytosterols (such as β-sitosterol) and fatty acids, which can competitively inhibit the activity of 5α-reductase, thereby reducing the conversion of testosterone to dihydrotestosterone (DHT), alleviating the attack and atrophy of hair follicles by DHT, and significantly improving androgenetic alopecia.

[0141] Biotin (vitamin B7), as a coenzyme of carboxylase, can directly participate in the metabolic processes of fats and proteins, especially the formation of keratin. Supplementing with biotin can significantly improve the problem of fragile and brittle hair, and increase hair shine and growth rate.

[0142] Dimethyl sulfone (MSM) is a natural organic sulfur donor. Sulfur is a key element in the formation of disulfide bonds in hair keratin. Sufficient sulfur can enhance the structural strength and elasticity of hair and prolong the duration of the hair growth phase.

[0143] Black sesame extract contains abundant melanin precursors, linoleic acid, and vitamin E, which can not only promote melanin production in hair and improve gray hair, but also moisturize the scalp, eliminate free radicals, and delay hair follicle aging.

Claims

1. A nutrient composition for promoting hair growth, characterized in that, Includes basic hair growth nutrients and absorption-enhancing synergistic nutrients; The components and weight percentages of the basic hair growth nutrient group include: 7-10 parts vitamin C, 0.3-0.8 parts ferrous lactate, 4-8 parts compound vitamin B powder, 2-5 parts zinc gluconate, 2-5 parts grape seed extract, 12-18 parts γ-aminobutyric acid, 5-8 parts magnesium stearate, 3-8 parts coating material, and 17-25 parts milk mineral salts. The components and weight proportions of the absorption-promoting synergistic group include: 0.2-1.5 parts of black pepper extract, 2-10 parts of lecithin, and 2-8 parts of casein phosphopeptide.

2. The nutrient composition for promoting hair growth according to claim 1, characterized in that, The black pepper extract contains ≥95% piperine; the lecithin is selected from soybean lecithin or egg yolk lecithin, wherein the acetone-insoluble content is ≥95%; and the total nitrogen content of the casein phosphopeptide is 10%-15%.

3. The nutrient composition for promoting hair growth according to claim 1, characterized in that, The grape seed extract contains ≥95% proanthocyanidins.

4. The nutrient composition for promoting hair growth according to claim 1, characterized in that, The purity of the γ-aminobutyric acid is ≥95%; the calcium content of the milk mineral salt is ≥25%.

5. A method for preparing a nutrient composition for promoting hair growth as described in any one of claims 1-4, characterized in that, Includes the following steps: Step S1, Raw material grouping: Black pepper extract and lecithin are separated and used as synergistic components; coating material and magnesium stearate are separated and used as base powder; the remaining raw materials are used as base powder. Step S2, Crushing and Sieving: The base powder is crushed, sieved and mixed evenly to obtain premixed powder; Step S3: Preparation of permeation-enhancing emulsifying adhesive: Dissolve and disperse black pepper extract and lecithin in an ethanol solution, and stir evenly to obtain permeation-enhancing emulsifying adhesive; Step S4, Granulation and Drying: The penetration-enhancing emulsified adhesive is sprayed into the premixed powder as a wetting agent to form a soft material and granulate it, followed by drying. Step S5, Granulation: Granulate the dried granules; Step S6, Mixing: Mix the granulated particles with the magnesium stearate taken out in step S1; Step S7, tableting: The mixed materials are compressed into tablets to obtain plain tablets; Step S8, Coating: The coating material taken out in step S1 is prepared into a coating solution, and the tablets after compression are coated to obtain a nutrient composition.

6. The preparation method according to claim 5, characterized in that, In step 2, the sieve mesh size is 80-100 mesh.

7. The preparation method according to claim 5, characterized in that, In step S3, the concentration of the ethanol solution is 30%-40%, and the black pepper extract and lecithin form a uniform emulsion dispersion system in the ethanol.

8. The preparation method according to claim 5, characterized in that, In step S4, the environmental conditions for the drying process are controlled as follows: temperature 50℃-60℃, humidity 2%-4%; in step S5, the mesh size of the granulated particles is controlled as 16-18 mesh.

9. The preparation method according to claim 5, characterized in that, In step S7, the tableting is carried out at a temperature of 18℃-24℃ and a relative humidity of 45%-55%, and the weight difference of the tablets after tableting is controlled within ±3%.

10. The preparation method according to claim 5, characterized in that, In step S8, the coating process is carried out under the conditions of an inlet air temperature of 70℃-80℃, an outlet air temperature of 40℃-55℃, and a coating pan rotation speed of 3-5 revolutions / minute, and the coating weight gain is controlled to be ±3%.

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