Hair follicle nourishing composition containing tripeptide-1 and preparation method thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses a hair follicle nourishing composition containing tripeptide-1 and a preparation method of the hair follicle nourishing composition. Comprising the following components in parts by mass: 36.5-47.3 parts of a Korean ginseng extract, 24-30 parts of a green alga extract, 20-28 parts of lactobacillus / soybean extract fermentation product filtrate, 1-5 parts of sodium polyglutamate, 1-4 parts of hydrogenated vegetable oil glyceride, 0.5-1.5 parts of sodium lactate and 0.2-1 part of tripeptide-1. On the basis that sodium polyglutamate, hydrogenated vegetable oil glycerides, sodium lactate and tripeptide-1 are added, through the synergistic interaction of the Korean ginseng extract, the green alga extract and the lactobacillus / soybean extract fermentation product filtrate, the composition capable of effectively inhibiting the activity of malassezia furfur, relieving or eliminating scalp inflammation, preventing and treating scalp inflammation and the like is obtained. Human hair papilla cell proliferation is promoted, and hair follicles are effectively nourished.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a hair follicle nourishing composition containing tripeptide-1 and its preparation method. Background Technology

[0002] As the foundation of hair growth, the health of hair follicles directly determines the growth, thickness, and resilience of hair. Abnormal hair follicle function can easily lead to problems such as hair loss, dry hair, itchy scalp, and increased dandruff, seriously affecting consumers' appearance and quality of life.

[0003] In recent years, due to factors such as increased life pressure, irregular work and rest, exposure to environmental pollutants, and improper scalp care, the incidence of hair follicle-related problems has been rising year by year and is showing a trend towards affecting younger people. Therefore, the development of compositions and related products that can effectively nourish hair follicles and improve the scalp microenvironment has become a research hotspot and market demand focus in the daily chemical industry. Summary of the Invention

[0004] This invention provides a hair follicle nourishing composition containing tripeptide-1 and its preparation method. The composition described in this application can effectively inhibit Malassezia furfur activity, relieve or eliminate scalp inflammation, promote the proliferation of human hair papilla cells, and effectively nourish hair follicles.

[0005] The present invention solves its technical problem by adopting the following technical solution: A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 36.5-47.3 parts Korean ginseng extract, 24-30 parts green algae extract, 20-28 parts Lactobacillus / soybean extract fermentation product filtrate, 1-5 parts sodium polyglutamate, 1-4 parts hydrogenated vegetable oil glycerides, 0.5-1.5 parts sodium lactate, and 0.2-1 parts tripeptide-1.

[0006] Among them, ginseng extract can disrupt the ergosterol synthesis pathway of fungal cell membranes, reduce cell membrane permeability, and inhibit fungal adhesion and reproduction; at the same time, it can downregulate the activity of lipase secreted by fungi, reduce the free fatty acids produced by the decomposition of scalp sebum (this substance is a key factor inducing scalp itching and inflammation), and it can also upregulate the expression of hair follicle growth-related factors (such as vascular endothelial growth factor VEGF and insulin-like growth factor IGF-1), prolonging the hair follicle growth phase.

[0007] Among them, the green algae extract can bind to the chitin in the fungal cell wall through electrostatic interaction, disrupting the integrity of the cell wall structure, inhibiting fungal DNA replication and protein synthesis, and enhancing the antibacterial effect; at the same time, it can scavenge reactive oxygen species produced by fungal metabolism, reducing oxidative stress damage to the scalp. Furthermore, the green algae extract can be directly used as a nutrient substrate to supplement the amino acids required for cell proliferation; it can inhibit apoptosis of human dermal papilla cells, improve cell survival rate, regulate the redox balance of the hair follicle microenvironment, and maintain the normal physiological function of human dermal papilla cells.

[0008] The fermentation product filtrate of the Lactobacillus / soybean extract can lower the pH value of the scalp microenvironment, which is unfavorable to the growth and reproduction of alkaliphilic Malassezia furfur. Simultaneously, the fermentation product can regulate the scalp flora structure, promote the colonization of beneficial bacteria, and inhibit the excessive proliferation of harmful bacteria. It also promotes the secretion of VEGF by human dermal papilla cells, improving blood circulation around hair follicles.

[0009] This application combines the above-mentioned raw materials. The composition, with the addition of sodium polyglutamate, hydrogenated vegetable oil glycerides, sodium lactate, and tripeptide-1, and through the synergistic effect of ginseng extract, green algae extract, and lactobacillus / soybean extract fermentation product filtrate, effectively inhibits Malassezia furfur activity, relieves or eliminates scalp inflammation, promotes the proliferation of human hair papilla cells, and effectively nourishes hair follicles.

[0010] As a preferred embodiment of the present invention, the components include the following parts by weight: 40-42.8 parts of Korean ginseng extract, 25-28 parts of green algae extract, 24-26 parts of lactobacillus / soybean extract fermentation product filtrate, 2-4 parts of sodium polyglutamate, 2-3 parts of hydrogenated vegetable oil glycerides, 0.8-1.2 parts of sodium lactate, and 0.4-0.8 parts of tripeptide-1.

[0011] As a preferred embodiment of the present invention, the components include the following parts by weight: 40 parts Korean ginseng extract, 25 parts green algae extract, 26 parts Lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0012] As a preferred embodiment of the present invention, the preparation method of the green algae extract is as follows: (1) Wash, dry and crush the green algae to obtain green algae powder; (2) The green algae powder was subjected to supercritical extraction to obtain the extract; (3) Add hemicellulase and trypsin to the extract, and perform enzymatic hydrolysis to obtain the hydrolysate; (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

[0013] In a preferred embodiment of the present invention, the supercritical extraction uses CO2 as the extractant and 50-70 wt% ethanol as the entrainer.

[0014] In a preferred embodiment of the present invention, the CO2 flow rate is 60~65 mL / min, the extraction temperature is 35~40℃, the extraction pressure is 20~25 MPa, and the extraction time is 40~80 min.

[0015] In a preferred embodiment of the present invention, the mass ratio of hemicellulase, trypsin, and extract is (0.005~0.01):(0.005~0.01):1.

[0016] In a preferred embodiment of the present invention, the enzymatic hydrolysis temperature is 45~52℃ and the time is 0.5~3h.

[0017] In a preferred embodiment of the present invention, the particle size of the green algae powder is 50-500 mesh.

[0018] As a preferred embodiment of the present invention, the amount of the hair follicle nourishing composition containing tripeptide-1 added to the cosmetic is 0.1~10%.

[0019] As a preferred embodiment of the present invention, the amount of the hair follicle nourishing composition containing tripeptide-1 added to the cosmetic is 0.2-2%.

[0020] As a preferred embodiment of the present invention, the amount of the hair follicle nourishing composition containing tripeptide-1 added to the cosmetic is 0.5-1%.

[0021] The present invention also provides a method for preparing a hair follicle nourishing composition containing tripeptide-1, comprising the following steps: weighing raw materials according to the ratio, mixing the raw materials evenly to obtain a hair follicle nourishing composition containing tripeptide-1.

[0022] The beneficial effects of the present invention are as follows: Based on the addition of sodium polyglutamate, hydrogenated vegetable oil glycerides, sodium lactate, and tripeptide-1, the composition described in this application, through the synergistic effect of ginseng extract, green algae extract, and fermentation product filtrate of lactobacillus / soybean extract, effectively inhibits Malassezia furfur activity, relieves or eliminates scalp inflammation, promotes the proliferation of human hair papilla cells, and effectively nourishes hair follicles. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] In this invention, the technical features described in an open-ended manner include both closed-ended technical solutions composed of the listed features and open-ended technical solutions that include the listed features.

[0025] In this invention, numerical ranges are involved. Unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe features or characteristics, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are included.

[0026] In this invention, there are no particular limitations on the specific dispersion and stirring methods.

[0027] Unless otherwise specified, all reagents or instruments used in this invention are commercially available conventional products. Unless otherwise specified, the raw materials used in each comparative example and the parallel experiments of each embodiment are the same commercially available products.

[0028] The ginseng extracts used in the embodiments and comparative examples of this invention were purchased from Xi'an Best Biotechnology Co., Ltd. The Lactobacillus / soybean extract fermentation product filtrate was purchased from Guangzhou Xiangtu Import & Export Trading Co., Ltd.

[0029] Sodium polyglutamate was purchased from Guangzhou Xufan Trading Co., Ltd.

[0030] Hydrogenated vegetable oil glycerides were purchased from BASF.

[0031] Tripeptide-1 was purchased from Shandong Jitai Biotechnology Co., Ltd.

[0032] Example 1 A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 40 parts Korean ginseng extract, 25 parts green algae extract, 26 parts Lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0033] The preparation method of the green algae extract is as follows: (1) Wash, dry and pulverize the green algae to 200 mesh to obtain green algae powder; (2) Add the green algae powder to the extraction vessel for supercritical extraction to obtain the extract; the supercritical extraction uses CO2 as the extractant and 65wt% ethanol as the entrainer, the CO2 flow rate is 65mL / min, the extraction temperature is 38℃, the extraction pressure is 24MPa, and the extraction time is 60min.

[0034] (3) Add hemicellulase and trypsin to the extract and hydrolyze at 48°C for 1 h to obtain the hydrolysate; the mass ratio of hemicellulase, trypsin and extract is 0.008:0.008:1.

[0035] (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

[0036] The preparation method of the hair follicle nourishing composition containing tripeptide-1 includes the following steps: weighing the raw materials according to the ratio, mixing the raw materials evenly, and obtaining the hair follicle nourishing composition containing tripeptide-1.

[0037] Example 2 A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 42.8 parts Korean ginseng extract, 28 parts green algae extract, 24 parts Lactobacillus / soybean extract fermentation product filtrate, 2 parts sodium polyglutamate, 2 parts hydrogenated vegetable oil glycerides, 0.8 parts sodium lactate, and 0.4 parts tripeptide-1.

[0038] The preparation method of the green algae extract is as follows: (1) Wash, dry and pulverize the green algae to 200 mesh to obtain green algae powder; (2) Add the green algae powder to the extraction vessel for supercritical extraction to obtain the extract; the supercritical extraction uses CO2 as the extractant and 65wt% ethanol as the entrainer, the CO2 flow rate is 65mL / min, the extraction temperature is 38℃, the extraction pressure is 24MPa, and the extraction time is 60min.

[0039] (3) Add hemicellulase and trypsin to the extract and hydrolyze at 48°C for 1 h to obtain the hydrolysate; the mass ratio of hemicellulase, trypsin and extract is 0.008:0.008:1.

[0040] (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

[0041] The preparation method of the hair follicle nourishing composition containing tripeptide-1 includes the following steps: weighing the raw materials according to the ratio, mixing the raw materials evenly, and obtaining the hair follicle nourishing composition containing tripeptide-1.

[0042] Example 3 A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 47.3 parts Korean ginseng extract, 30 parts green algae extract, 20 parts Lactobacillus / soybean extract fermentation product filtrate, 1 part sodium polyglutamate, 1 part hydrogenated vegetable oil glycerides, 0.5 parts sodium lactate, and 0.2 parts tripeptide-1.

[0043] The preparation method of the green algae extract is as follows: (1) Wash, dry and pulverize the green algae to 200 mesh to obtain green algae powder; (2) Add the green algae powder to the extraction vessel for supercritical extraction to obtain the extract; the supercritical extraction uses CO2 as the extractant and 65wt% ethanol as the entrainer, the CO2 flow rate is 65mL / min, the extraction temperature is 38℃, the extraction pressure is 24MPa, and the extraction time is 60min.

[0044] (3) Add hemicellulase and trypsin to the extract and hydrolyze at 48°C for 1 h to obtain the hydrolysate; the mass ratio of hemicellulase, trypsin and extract is 0.008:0.008:1.

[0045] (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

[0046] The preparation method of the hair follicle nourishing composition containing tripeptide-1 includes the following steps: weighing the raw materials according to the ratio, mixing the raw materials evenly, and obtaining the hair follicle nourishing composition containing tripeptide-1.

[0047] Example 4 A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 36.5 parts Korean ginseng extract, 24 parts green algae extract, 28 parts Lactobacillus / soybean extract fermentation product filtrate, 5 parts sodium polyglutamate, 4 parts hydrogenated vegetable oil glycerides, 1.5 parts sodium lactate, and 1 part tripeptide-1.

[0048] The preparation method of the green algae extract is as follows: (1) Wash, dry and pulverize the green algae to 200 mesh to obtain green algae powder; (2) Add the green algae powder to the extraction vessel for supercritical extraction to obtain the extract; the supercritical extraction uses CO2 as the extractant and 65wt% ethanol as the entrainer, the CO2 flow rate is 65mL / min, the extraction temperature is 38℃, the extraction pressure is 24MPa, and the extraction time is 60min.

[0049] (3) Add hemicellulase and trypsin to the extract and hydrolyze at 48°C for 1 h to obtain the hydrolysate; the mass ratio of hemicellulase, trypsin and extract is 0.008:0.008:1.

[0050] (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

[0051] The preparation method of the hair follicle nourishing composition containing tripeptide-1 includes the following steps: weighing the raw materials according to the ratio, mixing the raw materials evenly, and obtaining the hair follicle nourishing composition containing tripeptide-1.

[0052] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that the amounts of each raw material are different.

[0053] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 48.7 parts Korean ginseng extract, 35 parts green algae extract, 15 parts Lactobacillus / soybean extract fermentation product filtrate, 0.5 parts sodium polyglutamate, 0.5 parts hydrogenated vegetable oil glycerides, 0.2 parts sodium lactate, and 0.1 parts tripeptide-1.

[0054] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that the amounts of each raw material are different.

[0055] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 32.5 parts Korean ginseng extract, 20 parts green algae extract, 32 parts Lactobacillus / soybean extract fermentation product filtrate, 6 parts sodium polyglutamate, 6 parts hydrogenated vegetable oil glycerides, 2 parts sodium lactate, and 1.5 parts tripeptide-1.

[0056] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 does not contain ginseng extract (it is replaced by an equal amount of green algae extract and lactobacillus / soybean extract fermentation product filtrate).

[0057] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 45 parts green algae extract, 46 parts lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0058] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 does not contain green algae extract (it is replaced by an equal amount of Korean ginseng extract and lactobacillus / soybean extract fermentation product filtrate).

[0059] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 52.5 parts Korean ginseng extract, 38.5 parts Lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0060] Comparative Example 5 The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 does not contain Lactobacillus / soybean extract fermentation product filtrate (it is replaced with equal amounts of green algae extract and Korean ginseng extract).

[0061] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 53 parts Korean ginseng extract, 38 parts green algae extract, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0062] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that an equal amount of Ligusticum chuanxiong extract was used to replace the ginseng extract, and an equal amount of frankincense extract was used to replace the green algae extract.

[0063] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 40 parts Ligusticum chuanxiong extract, 25 parts frankincense extract, 26 parts Lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0064] Comparative Example 7 The difference between Comparative Example 7 and Example 1 is that an equal amount of Ligusticum chuanxiong extract was used to replace the ginseng extract, and an equal amount of Salvia miltiorrhiza extract was used to replace the Lactobacillus / soybean extract fermentation product filtrate.

[0065] A hair follicle nourishing composition containing tripeptide-1 comprises the following components in parts by weight: 40 parts Ligusticum chuanxiong extract, 25 parts Chlorella extract, 26 parts Salvia miltiorrhiza extract, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

[0066] Test case 1. The Malassezia furfur-containing hair follicle-nourishing composition containing tripeptide-1 was subjected to an inhibition test against Malassezia furfur (ATCC44344) in accordance with the "QBT2738-2012(7.3)-Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products".

[0067] 2. The CCK-8 assay was used to evaluate the promoting effect of the samples on the proliferation of human dermal papilla cells. The specific steps are as follows: human dermal papilla cells were divided into groups of 1×10⁻⁶ cells. 6 Human dermal papilla cells were seeded at a density of [number] cells / well in 96-well plates (referred to as the experimental group) and incubated overnight at 37°C with CO2. When the cell deposition rate reached 50%, 100 μL of DMEM high-glucose medium containing a hair follicle nourishing composition containing tripeptide-1 was added to each well. DMEM high-glucose medium without the tripeptide-1 nourishing composition was used as the control group. Each group had at least three replicates. Cells were incubated at 37°C for 48 hours. The relative viability (%) of human dermal papilla cells was determined using the CCK-8 assay. Relative viability (%) = 100%. (OD value of experimental group - OD value of control group) / OD value of control group.

[0068] Table 1

[0069] As can be seen from Table 1, the composition described in this application can effectively inhibit the activity of Malassezia furfur, relieve or eliminate scalp inflammation, promote the proliferation of human hair papilla cells, and effectively nourish hair follicles.

[0070] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A hair follicle nourishing composition containing tripeptide-1, characterized in that, The product comprises the following components in parts by weight: 36.5–47.3 parts Korean ginseng extract, 24–30 parts green algae extract, 20–28 parts Lactobacillus / soybean extract fermentation product filtrate, 1–5 parts sodium polyglutamate, 1–4 parts hydrogenated vegetable oil glycerides, 0.5–1.5 parts sodium lactate, and 0.2–1 part tripeptide-1.

2. The hair follicle nourishing composition containing tripeptide-1 according to claim 1, characterized in that, The product comprises the following components in parts by weight: 40-42.8 parts Korean ginseng extract, 25-28 parts green algae extract, 24-26 parts Lactobacillus / soybean extract fermentation product filtrate, 2-4 parts sodium polyglutamate, 2-3 parts hydrogenated vegetable oil glycerides, 0.8-1.2 parts sodium lactate, and 0.4-0.8 parts tripeptide-1.

3. The hair follicle nourishing composition containing tripeptide-1 according to claim 1, characterized in that, The product comprises the following components by weight: 40 parts Korean ginseng extract, 25 parts green algae extract, 26 parts Lactobacillus / soybean extract fermentation product filtrate, 4 parts sodium polyglutamate, 3 parts hydrogenated vegetable oil glycerides, 1.2 parts sodium lactate, and 0.8 parts tripeptide-1.

4. The hair follicle nourishing composition containing tripeptide-1 according to claim 1, characterized in that, The preparation method of the green algae extract is as follows: (1) Wash, dry and crush the green algae to obtain green algae powder; (2) The green algae powder was subjected to supercritical extraction to obtain the extract; (3) Add hemicellulase and trypsin to the extract, and perform enzymatic hydrolysis to obtain the hydrolysate; (4) The enzymatic hydrolysate was first ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 500 Da, and then ultrafiltered with an ultrafiltration membrane with a molecular weight cutoff of 5000 Da. After drying, the green algae extract was obtained.

5. The hair follicle nourishing composition containing tripeptide-1 according to claim 4, characterized in that, The supercritical extraction uses CO2 as the extractant and 50-70 wt% ethanol as the entrainer.

6. The hair follicle nourishing composition containing tripeptide-1 according to claim 5, characterized in that, The CO2 flow rate is 60~65 mL / min, the extraction temperature is 35~40℃, the extraction pressure is 20~25 MPa, and the extraction time is 40~80 min.

7. The hair follicle nourishing composition containing tripeptide-1 according to claim 5, characterized in that, The mass ratio of hemicellulase, trypsin, and extract is (0.005~0.01):(0.005~0.01):

1.

8. The hair follicle nourishing composition containing tripeptide-1 according to claim 5, characterized in that, The enzymatic hydrolysis is performed at a temperature of 45-52℃ for 0.5-3 hours.

9. The hair follicle nourishing composition containing tripeptide-1 according to claim 5, characterized in that, The particle size of the green algae powder is 50-500 mesh.

10. A method for preparing the hair follicle nourishing composition containing tripeptide-1 according to any one of claims 1 to 9, characterized in that, The process includes the following steps: weighing the raw materials according to the specified ratio, mixing the raw materials evenly to obtain a hair follicle nourishing composition containing tripeptide-1.

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