Plant essence lotion for promoting white hair to black hair as well as preparation method and application of plant essence lotion

Abstract

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a plant essence lotion for promoting white-to-black hair as well as a preparation method and application of the plant essence lotion. The plant essence lotion is prepared from the following raw materials in parts by mass: 25 to 35 parts of a traditional Chinese medicine compound, 8 to 10 parts of a humectant, 0.5 to 1 part of an antioxidant, 1 to 3 parts of a solubilizer, 0.1 to 0.3 part of a preservative and 50 to 80 parts of water, the traditional Chinese medicine compound comprises the following raw materials: ganoderma atrum, polygonum multiflorum, ginger root, cacumen biotae, rhizoma anemarrhenae root, ginseng, cordyceps militaris, black mulberry, polygonatum kingianum and salvia miltiorrhiza. The raw materials are combined, so that the hair follicle nourishing composition can take liver and kidney nourishing, essence and blood benefiting, blood circulation promoting, collateral dredging, oxidation resisting and hair follicle nourishing as the core, and by activating / protecting melanin cells and melanin stem cells, improving the activity of key enzymes such as tyrosinase and the like, improving scalp blood supply, providing sufficient nutrition for hair follicles and scavenging free radicals, so that the hair follicle nourishing composition can be used for nourishing hair follicles. Multi-dimensional synergistic effects of delaying hair follicle aging, regulating qi and blood of the body and the internal environment and the like are achieved, and the white hair is efficiently promoted to turn black.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic preparation technology, specifically relating to a plant essence emulsion for promoting the reversal of gray hair to black, its preparation method, and its application. Background Technology

[0002] Hair turning white is caused by the decline, depletion, or abnormal migration of melanocyte stem cells in hair follicles, resulting in insufficient number of melanocytes and decreased tyrosinase activity. Combined with factors such as genetics, oxidative stress, mental stress, endocrine disorders, and aging, hair eventually cannot synthesize melanin and turns white.

[0003] How to turn gray hair black is a research hotspot in this field. Currently, chemical hair dyes are the main method, but their side effects are significant and detrimental to people's health. Existing technologies also include reports on using plant-based ingredients to promote the blackening of gray hair. For example, Chinese patent CN 102764221 A describes a plant-based hair conditioner for turning gray hair black. This conditioner is prepared by mixing 1-2 parts by weight of gallnut, 1-2 parts by weight of processed Polygonum multiflorum powder, 35-40 parts by weight of henna leaf juice, 12-15 parts by weight of aloe vera leaf juice, 18-22 parts by weight of black bean juice, 12-14 parts by weight of privet fruit juice, 3-5 parts by weight of conditioning agent, and 1-2 parts by weight of triethanolamine. It is said to penetrate deep into the hair shaft by adsorption to the surface, gradually turning gray hair black from the roots. However, the effects of plant-based ingredients vary depending on their preparation methods and the interactions between them. Therefore, by using specific preparation methods and raw material mixing, a plant essence lotion that can effectively promote the reversal of gray hair to black can be obtained, which is of great significance for expanding the market. Summary of the Invention

[0004] The purpose of this invention is to provide a plant essence emulsion for promoting the reversal of gray hair to black, its preparation method and application. Through specific raw materials and preparation methods, it can effectively promote the reversal of gray hair to black.

[0005] This invention provides a plant essence lotion for promoting the reversal of gray hair to black. By weight, the plant essence lotion comprises the following ingredients: 25-35 parts of traditional Chinese medicine complex, 8-10 parts of moisturizer, 0.5-1 part of antioxidant, 1-3 parts of solubilizer, 0.1-0.3 parts of preservative, and 50-80 parts of water. The herbal compound includes the following ingredients: black Ganoderma lucidum, Polygonum multiflorum, ginger root, Platycladus orientalis leaf, Anemarrhena asphodeloides root, ginseng, Cordyceps militaris, black mulberry fruit, Polygonatum sibiricum and Salvia miltiorrhiza.

[0006] Preferably, the traditional Chinese medicine complex comprises the following raw materials in parts by weight: 5-8 parts of black Ganoderma lucidum, 3-8 parts of Polygonum multiflorum, 2-6 parts of ginger root, 15-20 parts of Platycladus orientalis leaf, 6-8 parts of Anemarrhena asphodeloides root, 2-4 parts of ginseng, 1-4 parts of Cordyceps militaris, 10-15 parts of black mulberry fruit, 4-8 parts of Polygonatum sibiricum and 0.5-2 parts of Salvia miltiorrhiza.

[0007] Preferably, the moisturizer includes: glycerin and / or panthenol; The antioxidants include: tocopheryl acetate and / or tea polyphenols; The solubilizer includes: PEG-40 hydrogenated castor oil and / or Tween 80; The preservatives include potassium sorbate or sodium benzoate.

[0008] This invention provides a method for preparing the plant essence emulsion described in the above technical solution, comprising the following steps: S1. Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng and Salvia miltiorrhiza are mixed and pulverized to obtain the first pulverized material; the first pulverized material is mixed with water and cooked at low temperature, cooled and filtered to obtain the first filtrate; the first filtrate, trehalose and Lactobacillus rhamnosus are subjected to anaerobic fermentation, and then Aspergillus oryzae is added for aerobic fermentation, centrifuged to obtain a precipitate; the precipitate, water and hyaluronic acid are mixed evenly and freeze-dried to obtain the first mixture; S2. Mix and pulverize Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum to obtain a second pulverized material; mix the second pulverized material with an ethanol solution of 35%-45% by volume for a first alcohol extraction, filter, and obtain a first alcohol extract and a first filter residue; mix the first filter residue with an ethanol solution of 85%-95% by volume for a second alcohol extraction, filter, and obtain a second alcohol extract and a second filter residue; combine the first alcohol extract and the second alcohol extract, and concentrate to 50%-60% of the original volume to obtain a second mixture; S3. Fresh arborvitae leaves, fresh black mulberry fruit, citric acid and water are mixed and cell wall broken to obtain a cell wall broken liquid; the cell wall broken liquid is subjected to gradient centrifugation to obtain a supernatant; the supernatant is mixed with pullulan polysaccharide and centrifuged to obtain a precipitate; the precipitate is freeze-dried to obtain a third mixture; S4. Combine the first mixture, the second mixture, and the third mixture to obtain the traditional Chinese medicine complex; mix the traditional Chinese medicine complex, moisturizer, antioxidant, solubilizer, preservative, and water to obtain the plant essence emulsion.

[0009] Preferably, in step S1, during anaerobic fermentation, the mass ratio of the first filtrate, trehalose, and Lactobacillus rhamnosus is 100:5-10:0.5-1; the anaerobic fermentation temperature is 32-37℃, and the time is 24-48h. During aerobic fermentation, the mass ratio of the first filtrate to Aspergillus oryzae is 100:0.06-0.1; The aerobic fermentation is carried out at a temperature of 32-37℃ for 12-24 hours. The effective viable counts of *Lactobacillus rhamnosus* and *Aspergillus oryzae* were ≥1×10⁻⁶ respectively. 8 cfu / g.

[0010] Preferably, in step S1, when performing low-temperature cooking, the mass ratio of the first pulverized material to water is 1:10-15; the low-temperature cooking temperature is 50-60℃, and the time is 6-8h. The mass ratio of the precipitate, water, and hyaluronic acid is 1:3-5:0.1-0.5.

[0011] Preferably, in step S2, the mass-to-volume ratio of the liquid to the material during the first and second alcohol extractions is 1g:8-10mL, respectively. The temperature for the first and second alcohol extractions is 25-30℃, and the time is 24-48h, respectively. The volume ratio of the first alcohol extract to the second alcohol extract is 1:1-1.2.

[0012] Preferably, in step S3, when mixing and breaking down the cell walls, the mass ratio of arborvitae leaves, citric acid, and water is 1:0.2-0.5:10-20; The mass ratio of the supernatant to pullulan is 1:0.2-0.6.

[0013] Preferably, in step S3, the gradient centrifugation includes: a first centrifugation, a second centrifugation, and a third centrifugation; The first centrifugation was performed at a speed of 800-1000g for 10-15 minutes. The second centrifugation was performed at a speed of 1200-1800g for 5-10 minutes. The third centrifugation is performed at a speed of 2000-2500g for 10-15 minutes.

[0014] This invention provides the application of the plant essence emulsion described in the above technical solution in the preparation of shampoo products.

[0015] Beneficial effects: This invention provides a plant essence lotion for promoting the reversal of gray hair to black. By weight, the plant essence lotion comprises the following ingredients: 25-35 parts of a traditional Chinese medicine complex, 8-10 parts of a moisturizer, 0.5-1 part of an antioxidant, 1-3 parts of a solubilizer, 0.1-0.3 parts of a preservative, and 50-80 parts of water. The traditional Chinese medicine complex comprises the following ingredients: black Ganoderma lucidum, Polygonum multiflorum, ginger root, Platycladus orientalis leaf, Anemarrhena asphodeloides root, ginseng, Cordyceps militaris, black mulberry fruit, Polygonatum sibiricum, and Salvia miltiorrhiza.

[0016] This invention utilizes black Ganoderma lucidum (Ganoderma lucidum) Black Ganoderma Polygonum multiflorum (He Shou Wu) Polygonum multiflorum ), ginger root ( Ginger officinalis ), Platycladus orientalis leaves ( Oriental platycladus Anemarrhena asphodeloides root ( Anemarrhena asphodel ), Ginseng ( Panax ginseng ), Cordyceps militaris ( Cordyceps militaris ), black mulberry ( Mulberry black ), Polygonatum ( Siberian Polygonum ) and Danshen ( Salvia miltiorrhiza The compound is made by combining traditional Chinese medicines. It can nourish the liver and kidneys, replenish essence and blood, promote blood circulation and unblock collaterals, resist oxidation and nourish hair follicles. It works synergistically in multiple dimensions, such as activating / protecting melanocytes and melanocyte stem cells, increasing the activity of key enzymes such as tyrosinase, improving scalp blood supply, providing sufficient nutrition to hair follicles, clearing free radicals, delaying hair follicle aging, and regulating the body's qi and blood and internal environment, so as to effectively promote the regeneration of gray hair to black. Detailed Implementation

[0017] In this invention, unless otherwise specified, the equipment, reagents and methods used are all conventional selections.

[0018] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0019] Example 1 The steps for preparing a plant-based essence lotion to promote the reversal of gray hair to black are as follows: Take 8g of black Ganoderma lucidum, 3g of Polygonum multiflorum, 5g of ginger root, 20g of Platycladus orientalis leaf, 8g of Anemarrhena asphodeloides root, 2g of ginseng, 1g of Cordyceps militaris, 10g of black mulberry fruit, 4g of Polygonatum sibiricum and 0.5g of Salvia miltiorrhiza, wash them clean and dry them for later use. S1 Prepares the first mixture The dried Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng, and Salvia miltiorrhiza were mixed and pulverized to obtain the first pulverized material (particle size <0.1cm); the first pulverized material was mixed with water at a mass ratio of 1:10, and boiled at 50℃ for 6 hours. After cooling to room temperature, it was filtered to obtain the first filtrate; the first filtrate, trehalose, and Lactobacillus rhamnosus were then added to the mixture. Lactobacillus buckthorn The effective viable bacteria count is 1×10⁻⁶. 8 CFU / g) were mixed at a mass ratio of 100:5:1 and anaerobic fermented at 32℃ for 48 h to obtain the anaerobic fermentation broth; the first filtrate was then mixed with Aspergillus oryzae (CFU / g). Aspergillus oryzae The effective viable bacteria count is 1×10⁻⁶. 8 Add Aspergillus oryzae to the anaerobic fermentation broth at a mass ratio of 100:0.1 (cfu / g) and ferment aerobically at 32°C for 24 hours to obtain an aerobic fermentation broth. Centrifuge the aerobic fermentation broth to obtain a precipitate. Mix the precipitate, water and hyaluronic acid at a mass ratio of 1:5:0.5 until homogeneous, and then freeze-dry to obtain the first mixture. S2 prepares the second mixture The dried Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum were mixed and pulverized to obtain a second pulverized material (particle size <0.1cm). The second pulverized material was mixed with a 35% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, a first ethanol extract and a first filter residue were obtained. The first filter residue was mixed with a 95% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, a second ethanol extract and a second filter residue were obtained. The first and second ethanol extracts were mixed at a volume ratio of 1:1 and concentrated to 50% of the original volume to obtain a second mixture. S3 prepares the third mixture Freshly dried arborvitae leaves, freshly dried black mulberries, citric acid, and water were mixed and cell wall-breaking (the mass ratio of arborvitae leaves, citric acid, and water was 1:0.5:20) to obtain a cell wall-breaking liquid. The cell wall-breaking liquid was subjected to a first centrifugation (800g, 10min), a second centrifugation (1200g, 5min), and a third centrifugation (2000g, 10min) to obtain a supernatant. The supernatant was mixed with prunol at a mass ratio of 1:0.2 and then freeze-dried to obtain a third mixture. S4 combines the first mixture, the second mixture, and the third mixture to obtain a traditional Chinese medicine complex; Take 35g of the Chinese herbal compound, 10g of moisturizer (glycerin), 1g of antioxidant (tocopheryl acetate), 1g of solubilizer (Tween 80), 0.1g of preservative (sodium benzoate), and 52.9g of water, mix them evenly to obtain the plant essence emulsion.

[0020] Example 2 The steps for preparing a plant-based essence lotion to promote the reversal of gray hair to black are as follows: Take 5g of black Ganoderma lucidum, 3g of Polygonum multiflorum, 5g of ginger root, 20g of Platycladus orientalis leaf, 8g of Anemarrhena asphodeloides root, 2g of ginseng, 1g of Cordyceps militaris, 10g of black mulberry fruit, 4g of Polygonatum sibiricum and 2g of Salvia miltiorrhiza, wash them clean and dry them for later use. S1 Prepares the first mixture The dried Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng, and Salvia miltiorrhiza were mixed and pulverized to obtain the first pulverized material (particle size <0.1cm); the first pulverized material was mixed with water at a mass ratio of 1:10, and boiled at 50℃ for 6 hours. After cooling to room temperature, it was filtered to obtain the first filtrate; the first filtrate, trehalose, and Lactobacillus rhamnosus were then added to the mixture. Lactobacillus buckthorn The effective viable bacteria count is 1×10⁻⁶. 8 CFU / g) were mixed at a mass ratio of 100:5:1 and anaerobic fermented at 32℃ for 48 h to obtain the anaerobic fermentation broth; the first filtrate was then mixed with Aspergillus oryzae (CFU / g). Aspergillus oryzae The effective viable bacteria count is 1×10⁻⁶.8 Add Aspergillus oryzae to the anaerobic fermentation broth at a mass ratio of 100:0.1 (cfu / g) and ferment aerobically at 32°C for 24 hours to obtain an aerobic fermentation broth. Centrifuge the aerobic fermentation broth to obtain a precipitate. Mix the precipitate, water and hyaluronic acid at a mass ratio of 1:5:0.5 until homogeneous, and then freeze-dry to obtain the first mixture. S2 prepares the second mixture The dried Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum were mixed and pulverized to obtain a second pulverized material (particle size <0.1cm). The second pulverized material was mixed with a 45% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, a first ethanol extract and a first filter residue were obtained. The first filter residue was mixed with an 85% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, a second ethanol extract and a second filter residue were obtained. The first and second ethanol extracts were mixed at a volume ratio of 1:1 and concentrated to 50% of the original volume to obtain a second mixture. S3 prepares the third mixture Freshly dried arborvitae leaves, freshly dried black mulberries, citric acid, and water were mixed and cell wall-breaking (the mass ratio of arborvitae leaves, citric acid, and water was 1:0.5:20) to obtain a cell wall-breaking liquid. The cell wall-breaking liquid was subjected to a first centrifugation (800g, 10min), a second centrifugation (1200g, 5min), and a third centrifugation (2000g, 10min) to obtain a supernatant. The supernatant was mixed with prunol at a mass ratio of 1:0.2 and then freeze-dried to obtain a third mixture. S4 combines the first mixture, the second mixture, and the third mixture to obtain a traditional Chinese medicine complex; Take 25g of the Chinese herbal compound, 10g of moisturizer (glycerin and panthenol mixed in a 1:1 mass ratio), 1g of antioxidant (tocopheryl acetate), 1g of solubilizer (Tween 80), 0.1g of preservative (sodium benzoate), and 62.9g of water, mix them evenly to obtain the plant essence emulsion.

[0021] Comparative Example 1 The steps for preparing a plant-based essence lotion to promote the reversal of gray hair to black are as follows: Take 8g of black Ganoderma lucidum, 3g of Polygonum multiflorum, 5g of ginger root, 20g of Platycladus orientalis leaf, 8g of Anemarrhena asphodeloides root, 2g of ginseng, 1g of Cordyceps militaris, 10g of black mulberry fruit, 4g of Polygonatum sibiricum and 0.5g of Salvia miltiorrhiza, wash them clean and dry them for later use. The dried Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng, Salvia miltiorrhiza, black Ganoderma lucidum, Cordyceps militaris, Polygonatum sibiricum, fresh Platycladus orientalis leaves, and fresh black mulberry fruit were mixed and pulverized to obtain the first pulverized material (particle size <0.1cm); the first pulverized material was mixed with water at a mass ratio of 1:10, and boiled at 50℃ for 6 hours. After cooling to room temperature, it was filtered to obtain the first filtrate; the first filtrate, trehalose, and Lactobacillus rhamnosus (… Lactobacillus rhamnosus The effective viable bacteria count is 1×10⁻⁶. 8 The mixture of CFU / g and hyaluronic acid was prepared at a mass ratio of 100:5:1 and anaerobic fermented at 32℃ for 48 hours to obtain a fermentation broth. The fermentation broth was centrifuged to obtain a precipitate. The precipitate, water and hyaluronic acid were mixed evenly at a mass ratio of 1:5:0.5 and then freeze-dried to obtain a traditional Chinese medicine complex. Take 35g of the Chinese herbal compound, 10g of moisturizer (glycerin), 1g of antioxidant (tocopheryl acetate), 1g of solubilizer (Tween 80), 0.1g of preservative (sodium benzoate), and 52.9g of water, mix them evenly to obtain the plant essence emulsion.

[0022] Comparative Example 2 The steps for preparing a plant-based essence lotion to promote the reversal of gray hair to black are as follows: Take 8g of black Ganoderma lucidum, 3g of Polygonum multiflorum, 5g of ginger root, 20g of Platycladus orientalis leaf, 8g of Anemarrhena asphodeloides root, 2g of ginseng, 1g of Cordyceps militaris, 10g of black mulberry fruit, 4g of Polygonatum sibiricum and 0.5g of Salvia miltiorrhiza, wash them clean and dry them for later use. S1 Prepares the first mixture The dried Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng, Salvia miltiorrhiza, Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum were mixed and pulverized to obtain the first pulverized material (particle size <0.1cm). The first pulverized material was mixed with a 35% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, the first ethanol extract and the first filter residue were obtained. The first filter residue was mixed with a 95% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, the second ethanol extract and the second filter residue were obtained. The first ethanol extract and the second ethanol extract were mixed at a volume ratio of 1:1 and concentrated to 50% of the original volume to obtain the first mixture. S2 prepares the second mixture Dried fresh arborvitae leaves, dried fresh black mulberries, citric acid, and water were mixed and cell wall-breaking (the mass ratio of arborvitae leaves, citric acid, and water was 1:0.5:20) to obtain a cell wall-breaking liquid. The cell wall-breaking liquid was subjected to a first centrifugation (800g, 10min), a second centrifugation (1200g, 5min), and a third centrifugation (2000g, 10min) to obtain a supernatant. The supernatant was mixed with prunol at a mass ratio of 1:0.2 and then freeze-dried to obtain a second mixture. S3 combines the first and second mixtures to obtain a traditional Chinese medicine complex; Take 35g of the Chinese herbal compound, 10g of moisturizer (glycerin), 1g of antioxidant (tocopheryl acetate), 1g of solubilizer (Tween 80), 0.1g of preservative (sodium benzoate), and 52.9g of water, mix them evenly to obtain the plant essence emulsion.

[0023] Comparative Example 3 The only difference from Example 1 is that the process of step S1 in Comparative Example 3 is as follows: S1 Prepares the first mixture The dried Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng, and Salvia miltiorrhiza were mixed and pulverized to obtain the first pulverized material (particle size <0.1cm); the first pulverized material was mixed with water at a mass ratio of 1:10, and boiled at 50℃ for 6 hours. After cooling to room temperature, it was filtered to obtain the first filtrate; the first filtrate was mixed at a mass ratio of 100:5:1 and anaerobic fermented at 32℃ for 48 hours to obtain the anaerobic fermentation broth; the first filtrate was then mixed with Aspergillus oryzae (… Aspergillus oryzae The effective viable bacteria count is 1×10⁻⁶. 8 Add Aspergillus oryzae to the anaerobic fermentation broth at a mass ratio of 100:0.1 (cfu / g) and ferment aerobically at 32°C for 24 hours to obtain an aerobic fermentation broth. Centrifuge the aerobic fermentation broth, filter it, concentrate and dry the filtrate to obtain the first mixture.

[0024] Comparative Example 4 The only difference from Example 1 is that the process of step S2 in Comparative Example 4 is as follows: S2 prepares the second mixture The dried Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum were mixed and pulverized to obtain a second pulverized material (particle size <0.1cm). The second pulverized material was mixed with a 65% ethanol solution at a mass-to-volume ratio of 1g:10mL and extracted with alcohol at 30℃ for 24h. After centrifugation and filtration, a first ethanol extract and a first filter residue were obtained. The first ethanol extract was concentrated to 50% of its original volume to obtain a second mixture. Comparative Example 5 The only difference from Example 1 is that the steps in Comparative Example 5 are as follows: Take 8g of black Ganoderma lucidum, 3g of Polygonum multiflorum, 5g of ginger root, 20g of fresh Platycladus orientalis leaves, 8g of Anemarrhena asphodeloides root, 2g of ginseng, 1g of Cordyceps militaris, 10g of fresh black mulberry fruit, 4g of Polygonatum sibiricum and 0.5g of Salvia miltiorrhiza, wash them clean and dry them for later use. The above raw materials were mixed, dried at 55°C, and pulverized to obtain a traditional Chinese medicine complex (the particle size of the traditional Chinese medicine complex is ≤0.05cm). Take 35g of the Chinese herbal compound, 10g of moisturizer (glycerin), 1g of antioxidant (tocopheryl acetate), 1g of solubilizer (Tween 80), 0.1g of preservative (sodium benzoate), and 52.9g of water, mix them evenly to obtain the plant essence emulsion.

[0025] Verification Example The plant extract emulsions prepared in Examples 1-2 and Comparative Examples 1-5 were verified as follows; (1) Tyrosinase activation rate Using phosphate buffer as a solvent, an L-tyrosine solution with a concentration of 0.5 mg / mL and a mushroom tyrosinase solution with a concentration of 35 mg / mL were prepared. The above plant essence emulsion was diluted with propylene glycol as a solvent to obtain a test solution with a concentration of 1 mg / mL.

[0026] Prepare the solutions for each group as follows: A: 0.7 mL L-tyrosine solution + 0.7 mL pure propylene glycol + 0.6 mL phosphate buffer; B: 0.7 mL pure propylene glycol + 1.3 mL phosphate buffer; C: 0.7 mL L-tyrosine solution + 0.7 mL test solution + 0.6 mL phosphate buffer; D: 0.7 mL test solution + 0.6 mL phosphate buffer; In the validation example, the pH of the phosphate buffer was 6.5-6.8; At 37℃, the solution of the AD group was placed in a water bath for 10 min, and 0.3 mL of tyrosinase solution was added to each group. After reacting for 5 min, the absorbance value (OD value) at 475 nm was measured, and the tyrosinase activation rate was calculated. Tyrosinase activation rate = 100% × (OD C -OD D )-(OD A -OD B )) / (OD A -OD B Each test solution was tested in three parallel replicates, and the results are shown in Table 1.

[0027] (2) Melanin content The concentration is 1×10 5 A suspension of B16 melanoma cells per mL was seeded into 12-well plates (1 mL per well, ensuring a cell count of 5 × 10⁶ cells per well). 4 (each) was cultured at 37℃ and 5% CO2 for 24 hours, and then the culture medium was removed; Test group: Add 1 mL of culture medium containing 1.5 mg / mL of each plant essence emulsion to the test group; Add an equal amount of culture medium without the plant essence emulsion to the blank control group and the zeroing group, continue culturing for 12 h, and then remove the culture medium.

[0028] Add 1 mL of 1 μM α solution prepared with RPM I 1640 medium to each well of both the test group and the zeroing group. MSH solution, blank control group added 1 mL of solution without α The cells were cultured in MSH medium for 36 hours. After washing twice with PBS (1 mL), 100 μL of 1N NaOH aqueous solution containing 10 wt% DMSO was added to each well. The cells were then incubated at 80°C for 1 hour. The cell lysates were transferred to 96-well plates, and the absorbance (OD) of each well was measured at 475 nm using a microplate reader. The intracellular melanin content (Q) was calculated. The results are shown in Table 1.

[0029] Where Q=(OD) 待测组 -OD 调零组 ) / (OD 空白对照组 -OD 调零组 )×100%.

[0030] Table 1. Tyrosinase activation rate and melanin content in different treatments

[0031] As shown in Table 1, compared with the comparative examples, the plant extract emulsions prepared in Examples 1 and 2 have a higher tyrosinase activation rate, specifically 49.3%-50.4%; and a Q value of 19.9%-20.32%. Therefore, the plant extract emulsions prepared in these examples are beneficial for promoting melanin production.

[0032] (3) Animal verification Several healthy black-and-white guinea pigs (weighing 900-950g) were taken and acclimatized for one week. After no abnormalities were observed, the following verification was performed: Long hair was removed from the black area on the back of black-spotted guinea pigs (without damaging the skin); and the guinea pigs were randomly divided into experimental groups (specifically, Example 1, Example 2, Comparative Example 1, Comparative Example 2... Comparative Example 5), model control group, and normal control group, with 10 black-spotted guinea pigs in each group. The test area of ​​the guinea pigs was treated with 5% hydrogen peroxide for 40 days, twice a day, 2 mL each time, until the hair turned white.

[0033] After successful modeling, mice in the experimental group were treated with 2 mL of the aforementioned plant extract emulsion daily (application sites were marked during the experiment), while the model control group received the same amount of water daily. The normal control group received no treatment. The experimental period was 15 days. During the experimental period, all black-and-white guinea pigs were observed for any abnormalities (e.g., skin redness and swelling, loss of appetite, etc.). 24 hours after the last administration, three black-and-white guinea pigs from each treatment were randomly selected, and a 5 cm band was drawn around the application site. 2 The average black hair percentage (average black hair percentage (%) = sum of black hair percentages of 3 black-and-white guinea pigs / 3; black hair percentage (%) of a single black-and-white guinea pig = number of black hairs / total number of hairs × 100%) was statistically analyzed, and the results were rounded to one decimal place, as shown in Table 2.

[0034] Table 2. Average black hair rate and abnormalities in different treatments

[0035] As shown in Table 2, the average black hair rate in the examples was as high as 82.5%-83.6%. This indicates that applying the plant essence emulsion prepared in the examples to animal hair can effectively promote the reversion of gray hair caused by external oxidative factors to black.

[0036] In summary, this invention mixes 25-35 parts of a traditional Chinese medicine complex, 8-10 parts of a moisturizer, 0.5-1 part of an antioxidant, 1-3 parts of a solubilizer, 0.1-0.3 parts of a preservative, and 50-80 parts of water. The traditional Chinese medicine complex includes the following ingredients: black Ganoderma lucidum, Polygonum multiflorum, ginger root, Platycladus orientalis leaf, Anemarrhena asphodeloides root, ginseng, Cordyceps militaris, black mulberry fruit, Polygonatum sibiricum, and Salvia miltiorrhiza. These ingredients, with the core functions of tonifying the liver and kidneys, nourishing essence and blood, promoting blood circulation, anti-oxidation, and nourishing hair follicles, work synergistically across multiple dimensions, including activating / protecting melanocytes and melanocyte stem cells, increasing the activity of key enzymes such as tyrosinase, improving scalp blood supply, providing sufficient nutrition to hair follicles, scavenging free radicals, delaying hair follicle aging, and regulating the body's Qi and blood and internal environment, thus effectively promoting the reversion of gray hair to black.

[0037] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A plant-based essence lotion for promoting the reversal of gray hair to black, characterized in that, The plant essence emulsion comprises the following ingredients by weight: 25-35 parts of traditional Chinese medicine complex, 8-10 parts of moisturizer, 0.5-1 part of antioxidant, 1-3 parts of solubilizer, 0.1-0.3 parts of preservative and 50-80 parts of water; The herbal compound includes the following ingredients: black Ganoderma lucidum, Polygonum multiflorum, ginger root, Platycladus orientalis leaf, Anemarrhena asphodeloides root, ginseng, Cordyceps militaris, black mulberry fruit, Polygonatum sibiricum and Salvia miltiorrhiza.

2. The plant essence emulsion according to claim 1, characterized in that, The traditional Chinese medicine complex comprises the following raw materials by weight: 5-8 parts of black Ganoderma lucidum, 3-8 parts of Polygonum multiflorum, 2-6 parts of ginger root, 15-20 parts of Platycladus orientalis leaf, 6-8 parts of Anemarrhena asphodeloides root, 2-4 parts of ginseng, 1-4 parts of Cordyceps militaris, 10-15 parts of black mulberry fruit, 4-8 parts of Polygonatum sibiricum and 0.5-2 parts of Salvia miltiorrhiza.

3. The plant essence emulsion according to claim 1, characterized in that, The moisturizer includes: glycerin and / or panthenol; The antioxidants include: tocopheryl acetate and / or tea polyphenols; The solubilizer includes: PEG-40 hydrogenated castor oil and / or Tween 80; The preservatives include potassium sorbate or sodium benzoate.

4. The method for preparing the plant extract emulsion according to any one of claims 1-3, characterized in that, Includes the following steps: S1. Polygonum multiflorum, ginger root, Anemarrhena asphodeloides root, ginseng and Salvia miltiorrhiza are mixed and pulverized to obtain the first pulverized material; the first pulverized material is mixed with water and cooked at low temperature, cooled and filtered to obtain the first filtrate; the first filtrate, trehalose and Lactobacillus rhamnosus are mixed and anaerobic fermented, then Aspergillus oryzae is added for aerobic fermentation, centrifuged to obtain a precipitate; the precipitate, water and hyaluronic acid are mixed evenly and freeze-dried to obtain the first mixture; S2. Mix and pulverize Ganoderma lucidum, Cordyceps militaris, and Polygonatum sibiricum to obtain a second pulverized material; mix the second pulverized material with an ethanol solution of 35%-45% by volume for a first alcohol extraction, filter, and obtain a first alcohol extract and a first filter residue; mix the first filter residue with an ethanol solution of 85%-95% by volume for a second alcohol extraction, filter, and obtain a second alcohol extract and a second filter residue; combine the first alcohol extract and the second alcohol extract, and concentrate to 50%-60% of the original volume to obtain a second mixture; S3. Fresh arborvitae leaves, fresh black mulberry fruit, citric acid and water are mixed and cell wall broken to obtain a cell wall broken liquid; the cell wall broken liquid is subjected to gradient centrifugation to obtain a supernatant; the supernatant is mixed with pullulan polysaccharide and centrifuged to obtain a precipitate; the precipitate is freeze-dried to obtain a third mixture; S4. Combine the first mixture, the second mixture, and the third mixture to obtain the traditional Chinese medicine complex; mix the traditional Chinese medicine complex, moisturizer, antioxidant, solubilizer, preservative, and water to obtain the plant essence emulsion.

5. The preparation method according to claim 4, characterized in that, In step S1, during anaerobic fermentation, the mass ratio of the first filtrate, trehalose, and Lactobacillus rhamnosus is 100:5-10:0.5-1; the temperature for anaerobic fermentation is 32-37℃, and the time is 24-48h. During aerobic fermentation, the mass ratio of the first filtrate to Aspergillus oryzae is 100:0.06-0.1; The aerobic fermentation is carried out at a temperature of 32-37℃ for 12-24 hours. The effective viable counts of *Lactobacillus rhamnosus* and *Aspergillus oryzae* were ≥1×10⁻⁶ respectively. 8 cfu / g.

6. The preparation method according to claim 4, characterized in that, In step S1, during the low-temperature cooking, the mass ratio of the first pulverized material to water is 1:10-15; the low-temperature cooking temperature is 50-60℃, and the time is 6-8 hours. The mass ratio of the precipitate, water, and hyaluronic acid is 1:3-5:0.1-0.

5.

7. The preparation method according to claim 4, characterized in that, In step S2, the mass-to-volume ratio of the material to the liquid is 1g:8-10mL during the first and second alcohol extractions. The temperature for the first and second alcohol extractions is 25-30℃, and the time is 24-48h, respectively. The volume ratio of the first alcohol extract to the second alcohol extract is 1:1-1.

2.

8. The preparation method according to claim 4, characterized in that, In step S3, during the mixing and cell wall disruption, the mass ratio of arborvitae leaves, citric acid, and water is 1:0.2-0.5:10-20. The mass ratio of the supernatant to pullulan is 1:0.2-0.

6.

9. The preparation method according to claim 4, characterized in that, In step S3, the gradient centrifugation includes: a first centrifugation, a second centrifugation, and a third centrifugation; The first centrifugation was performed at a speed of 800-1000g for 10-15 minutes. The second centrifugation was performed at a speed of 1200-1800g for 5-10 minutes. The third centrifugation is performed at a speed of 2000-2500g for 10-15 minutes.

10. The use of the plant extract emulsion according to any one of claims 1-3 in the preparation of shampoo products.

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