Nutrition regulator based on bioactive substances and preparation method thereof

Abstract

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

Description

Technical Field

[0001] This invention relates to the field of aquaculture water quality control technology, specifically to a method for preparing a nutrient regulator based on bioactive substances. This nutrient regulator is suitable for algae cultivation, water quality optimization, and pest control in marine and freshwater aquaculture bodies. Background Technology

[0002] In aquaculture, the stability of the aquatic environment directly affects the growth and survival rate of farmed animals. Traditional fertilization methods often rely on chemical fertilizers (such as urea and superphosphate) or single organic fertilizers, which have problems such as poor water solubility, short-lived fertilization effects, and algal imbalance.

[0003] Poor water solubility: Traditional fertilizers tend to settle, leading to the accumulation of organic matter at the bottom layer and causing deterioration of the substrate;

[0004] Short-lived fertilizer effect: Nutrients are released quickly but the effect is short-lived, requiring frequent fertilization and increasing breeding costs;

[0005] Algal imbalance: lack of directional regulation ability, which can easily lead to the outbreak of harmful algae such as blue-green algae and dinoflagellates. At the same time, the growth of green moss leads to a decrease in water light transmittance, which affects the growth of aquatic plants and beneficial algae.

[0006] Therefore, developing a nutrient regulator that is highly water-soluble, has a long-lasting fertilizer effect, and can selectively cultivate beneficial algae while inhibiting harmful organisms has become an urgent need in the aquaculture industry. Summary of the Invention

[0007] In view of this, the present invention proposes a method for preparing a nutrient regulator based on bioactive substances to solve the above problems.

[0008] The technical solution of this invention is implemented as follows:

[0009] A nutritional regulator based on bioactive substances comprises the following raw materials in parts by weight: 10-20 parts yeast polysaccharide, 15-25 parts fulvic acid, 20-30 parts highly active carbon source, 5-10 parts compound biological enzyme, 8-15 parts algal growth factor, 8-12 parts compound probiotics, 3-8 parts betaine, 15-25 parts compound mineral elements, and 5-10 parts compound trace elements;

[0010] Furthermore, the algal growth factor is composed of wakame ferment, pomegranate peel ferment, and seaweed ferment in a mass ratio of 4-6:3-5:1-2.

[0011] Furthermore, the highly active carbon source is composed of glucose and sucrose in a mass ratio of 1.0:1.0-1.5.

[0012] Furthermore, the complex bio-enzyme is composed of protease, amylase, and cellulase in a mass ratio of 5-7:2-4:1-2.

[0013] Furthermore, the protease is composed of neutral protease and alkaline protease in a mass ratio of 7-8:2-3.

[0014] Furthermore, the wakame fermented material is prepared by the following method: Select pollution-free and mold-free dried wakame and pulverize it to 60-80 mesh to obtain wakame powder; combine Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum in a mass ratio of 1.8-2.2:2.0-2.5:0.8-1.2 to obtain compound fermentation agent I; add water at 35-37℃ at a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL), then add 1.0-1.5 times the mass of compound fermentation agent I with a 4-6% brown sugar solution, and activate for 25-35 minutes to obtain activated compound fermented material I; mix wakame powder, brown sugar, yeast extract, potassium dihydrogen phosphate, and purified water in a mass ratio of 10-15:1-2:0.3-0.5:0.1:80-90, and stir evenly to obtain fermented material I; add the fermented material... Add 1-3% by volume of activated compound fermentation agent I to I, stir evenly, and ferment at 33-37℃ for 48-72 hours. For the first 12 hours, stir fermentation at 40-60 rpm, and then let it stand for fermentation with aeration for 10-15 minutes every 12 hours. After fermentation, raise the temperature to 80-85℃ and maintain it for 15-20 minutes. Then, perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 35-50% of the original volume at 50-60℃ to obtain concentrated liquid I. Then, add 1.5-2.0 times the mass of protective agent I to concentrated liquid I, stir and mix evenly, and then homogenize 2-3 times under a pressure of 30-40 MPa. Then, perform vacuum belt heating with a heating plate temperature of 65-75℃ and a vacuum degree of -0.06 to -0.08 MPa. After drying, pulverize and pass through a 60-100 mesh sieve to obtain wakame fermentation material.

[0015] Furthermore, Protective Agent I is composed of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 6.0-8.0:1.5-2.5:0.8-1.2.

[0016] Furthermore, the pomegranate peel ferment is prepared by the following method: Select pollution-free and mold-free dried pomegranate peels, pulverize them to 40-60 mesh to obtain pomegranate peel powder; combine Bacillus subtilis, Aspergillus niger, and Lactobacillus plantarum in a mass ratio of 2.5-3:0.9-1.1:0.8-1.0 to obtain compound fermentation agent II; add water at 30-35℃ in a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL); then add 1.0-1.5 times the mass of compound fermentation agent II in a 4-6% brown sugar solution, and activate for 40-50 minutes to obtain activated compound fermentation agent II; mix pomegranate peel powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 8-12:2-3:0.5-0.8:0.1:0.05:85-95, stir evenly, and obtain fermentation material II; Add 2-4% by volume of activated compound fermentation agent II to fermentation material II, stir evenly, and ferment at 29-31℃ for 96-120h. For the first 24h, stir fermentation at 40-60rpm and aerate for 4-6min every 6h. Then allow static fermentation. After fermentation, raise the temperature to 80-85℃ and maintain it for 15-20min. Then perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 30-40% of the original volume at 50-55℃ to obtain concentrated liquid II. Add 1.5-2.0 times the mass of protective agent II to concentrated liquid II, stir and mix evenly, and then homogenize 2-3 times under 30-40MPa pressure. Then perform vacuum belt heating with heating plate temperature of 60-70℃ and vacuum degree of -0.06~-0.08MPa. After drying, pulverize and pass through a 60-100 mesh sieve to obtain pomegranate peel fermentation material.

[0017] Furthermore, Protectant I is composed of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 5.0-7.0:2.0-3.0:0.8-1.2.

[0018] Furthermore, the *Ulva prolifera* fermented material is prepared by the following method: Dry, unrotten *Ulva prolifera* is selected and pulverized to 80-100 mesh to obtain *Ulva prolifera* powder; *Bacillus subtilis*, *Bacillus belye*, and *Candida utilis* are compounded at a mass ratio of 1.8-2.2:1.4-1.6:0.8-1.0 to obtain compound fermentation agent III; water at 30-32℃ is added at a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL); then, 1.0-1.5 times the mass of compound fermentation agent III is added to a 4-6% brown sugar solution, and the mixture is activated for 55-65 minutes to obtain activated compound fermentation agent III; *Ulva prolifera* powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate, and purified water are mixed at a mass ratio of 15-20:1.0-1.5:0.2:0.3-0.5:0.5-1.0:75-85 and stirred evenly to obtain fermentation material III; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Add 3-5% by volume of activated compound fermentation product III to product III, stir evenly, and ferment at 27-29℃ for 72-96 hours. For the first 48 hours, stir at 40-60 rpm and aerate for 20-30 minutes every 8 hours. For the next 12 hours, aerate for 8-12 minutes while stirring. After fermentation, raise the temperature to 80-85℃ and maintain for 15-20 minutes. Then, perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 40-50% of its original volume at 52-58℃ to obtain concentrated product III. Add 1.2-1.8 times its mass of protective agent III to concentrated product III, stir and mix evenly, and then homogenize 2-3 times under 30-40 MPa pressure. Then, perform vacuum belt heating with a heating plate temperature of 62-68℃ and a vacuum degree of -0.06 to -0.08 MPa. After drying, pulverize through a 60-100 mesh sieve to obtain the Ulva prolifera fermentation product.

[0019] Furthermore, the protective agent III is composed of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 7.0-8.0:1.0-1.5:1.0-1.5.

[0020] Furthermore, the compound probiotics consist of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 3.5-4.5:3.0-4.0:2.0-3.0.

[0021] Furthermore, the composite mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate, and sodium silicate in a mass ratio of 8-12:6-9:4-6:3-5.

[0022] Furthermore, the complex trace elements consist of sodium iron EDTA, manganese EDTA, zinc EDTA, and selenomethionine in a mass ratio of 4.5-5.5:2.0-3.0:1.5-2.5:0.1-0.3.

[0023] Furthermore, the method for preparing a nutrient regulator based on bioactive substances includes the following steps:

[0024] S1. Mix the composite mineral elements, composite trace elements, highly active carbon source, and fulvic acid evenly to obtain mixture I;

[0025] S2. Add yeast polysaccharide, compound biological enzyme, algae growth factor and betaine to mixture I, stir and mix evenly to obtain mixture II;

[0026] S3. Add compound probiotics to mixture II, stir and mix evenly, then package to obtain a nutritional regulator.

[0027] Furthermore, in S1, the stirring speed is 150-250 rpm and the stirring time is 15-25 min; in S2, the stirring speed is 80-150 rpm and the stirring time is 20-30 min; and in S3, the stirring speed is 60-100 rpm and the stirring time is 8-15 min.

[0028] Furthermore, the nutrient regulator is suitable for both marine and freshwater aquaculture waters.

[0029] Compared with the prior art, the beneficial effects of the present invention are:

[0030] 1. The nutrient regulator of this invention provides a safe and long-lasting ecological solution for the control of harmful organisms such as cyanobacteria, avoiding the side effects of chemical drugs. Through a compound algae growth regulator composed of wakame ferment, pomegranate peel ferment, and Ulva ferment, the three work synergistically. Wakame ferment promotes diatom growth, Ulva ferment promotes green algae growth, and pomegranate peel ferment inhibits the growth of harmful algae such as cyanobacteria, thereby increasing the proportion of beneficial algae such as diatoms and maintaining this proportion long-term. This results in a stable, efficient, and aquaculture-friendly high-quality water color, fundamentally optimizing the productivity structure of the pond.

[0031] 2. The nutrient regulator of this invention constructs a closed-loop system of "nutrient supply-biotransformation-slow-release utilization," overcoming the shortcomings of traditional fertilizers, such as short-term and fluctuating effects. The compound probiotics, compound enzymes, fulvic acid, and highly active carbon source in the nutrient regulator work synergistically. Specifically, the compound probiotics and compound enzymes continuously decompose organic waste (uneaten feed, feces) in the water and sediment, converting it into inorganic salts that algae can directly utilize, thus "turning waste into treasure." Simultaneously, fulvic acid integrates mineral and trace elements to form a slow-release nutrient reservoir; the highly active carbon source provides the energy source.

[0032] 3. The nutritional formula of this invention provides balanced and easily absorbed nutrients required for the growth of algae and probiotics, especially sufficient silicon to directly meet the growth needs of diatoms. Yeast polysaccharides can enhance the non-specific immunity of farmed animals; betaine, as a highly efficient osmotic pressure regulator, can significantly improve the survival rate of algae and aquatic animals under stresses such as sudden changes in salinity and temperature. Detailed Implementation

[0033] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0034] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0035] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0036] Example 1

[0037] A nutritional regulator based on bioactive substances comprises the following raw materials in parts by weight: 10 parts yeast polysaccharide, 15 parts fulvic acid, 20 parts highly active carbon source, 5 parts compound biological enzyme, 8 parts algal growth factor, 8 parts compound probiotics, 3 parts betaine, 15 parts compound mineral elements, and 5 parts compound trace elements. The high-activity carbon source consists of glucose and sucrose in a mass ratio of 1.0:1.0; the complex bio-enzyme consists of protease, amylase, and cellulase in a mass ratio of 5:2:1, with the protease consisting of neutral protease and alkaline protease in a mass ratio of 7:2; the algal growth factor consists of wakame ferment, pomegranate peel ferment, and seaweed ferment in a mass ratio of 4:3:1; the complex probiotic consists of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 3.5:3.0:2.0; the complex mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate, and sodium silicate in a mass ratio of 8:6:4:3; and the complex trace elements consist of EDTA iron sodium, EDTA manganese, EDTA zinc, and selenomethionine in a mass ratio of 4.5:2.0:1.5:0.1.

[0038] The wakame fermented material is prepared by the following method: Select pollution-free and mold-free dried wakame and pulverize it to 60 mesh to obtain wakame powder; Combine Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum in a mass ratio of 1.8:2.0:0.8 to obtain compound fermentation agent I; add water at 35℃ at a material-to-liquid ratio of 1:6 (material-to-liquid ratio in g / mL); then add 1.0 times the mass of compound fermentation agent I with a 4% brown sugar solution and activate for 35 minutes to obtain activated compound fermented material I; mix wakame powder, brown sugar, yeast extract, potassium dihydrogen phosphate, and purified water in a mass ratio of 10:1:0.3:0.1:80 and stir evenly to obtain fermented material I; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Activated compound fermenter I (1% by volume) was added to fermentation material I, stirred evenly, and fermented at 33℃ for 72 hours. For the first 12 hours, fermentation was carried out with stirring at 40 rpm, followed by static fermentation with aeration for 10 minutes every 12 hours. After fermentation, the temperature was raised to 80℃ and maintained for 20 minutes, then solid-liquid separation was performed. The filtrate was collected and concentrated to 50% of its original volume at 50℃ to obtain concentrated liquid I. Then, 1.5 times its weight of protective agent I was added to concentrated liquid I, stirred evenly, and homogenized three times under 30 MPa pressure. Vacuum belt heating was then performed at 65℃ and a vacuum degree of -0.06 MPa. After drying, the mixture was pulverized through a 60-mesh sieve to obtain the wakame seaweed fermented product. Protective agent I consisted of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 6.0:1.5:0.8.

[0039] The pomegranate peel ferment is prepared by the following method: Select pollution-free and mold-free dried pomegranate peels, pulverize them to 40 mesh to obtain pomegranate peel powder; combine Bacillus subtilis, Aspergillus niger, and Lactobacillus plantarum in a mass ratio of 2.5:0.9:0.8 to obtain compound fermentation agent II; add water at 30℃ in a material-to-liquid ratio of 1:6 (material-to-liquid ratio in g / mL); then add 1.0 times the mass of compound fermentation agent II in a 4% brown sugar solution, and activate for 50 minutes to obtain activated compound fermentation agent II; mix pomegranate peel powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 8:2:0.5:0.1:0.05:85, stir evenly, and obtain the fermented product. Material II; Add 2% by volume of activated compound fermentation agent II to fermentation material II, stir evenly, and ferment at 29℃ for 120h. For the first 24h, stir fermentation at 40rpm and aerate for 6min every 6h. Then allow static fermentation. After fermentation, raise the temperature to 80℃ and maintain for 20min, then perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 40% of the original volume at 50℃ to obtain concentrated liquid II. Add 1.5 times the mass of protective agent II to concentrated liquid II, stir and mix evenly, then homogenize 3 times at 30MPa pressure, then perform vacuum belt heating at 60℃ and vacuum degree -0.06MPa. After drying, pulverize and pass through a 60-mesh sieve to obtain pomegranate peel fermentation product. Among them, protective agent I is composed of maltodextrin, β-cyclodextrin and soluble starch in a mass ratio of 5.0:2.0:0.8.

[0040] The *Ulva prolifera* fermented material was prepared by the following method: Dry, unrotten *Ulva prolifera* was selected and pulverized to 80 mesh to obtain *Ulva prolifera* powder; *Bacillus subtilis*, *Bacillus belye*, and *Candida utilis* were compounded in a mass ratio of 1.8:1.4:0.8 to obtain compound fermentation agent III; water at 30℃ was added at a material-to-liquid ratio of 1:6 (material-to-liquid ratio in g / mL); then, 1.0 times the mass of compound fermentation agent III (4% brown sugar solution) was added, and the mixture was activated for 65 minutes to obtain activated compound fermentation agent III; *Ulva prolifera* powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate, and purified water were mixed in a mass ratio of 15:1.0:0.2:0.3:0.5:75 and stirred evenly to obtain fermentation material III; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Add 3% by volume of activated compound fermentation agent III to ingredient III, stir well, and ferment at 27℃ for 96 hours. For the first 48 hours, stir at 40 rpm and aerate for 30 minutes every 8 hours. For the subsequent 48 hours, aerate for 12 minutes every 12 hours while maintaining stirring. After fermentation, raise the temperature to 80℃ and hold for 20 minutes. Then, perform solid-liquid separation, collect the filtrate, and concentrate it to 50% of its original volume at 52℃ to obtain concentrated liquid III. Add 1.2 times its mass of protective agent III to concentrated liquid III, stir well, and then homogenize three times at 30 MPa. Then, perform vacuum belt heating at 62℃ and a vacuum of -0.06 MPa. After drying, pulverize and pass through a 60-mesh sieve to obtain the *Ulva prolifera* fermentation product. Protective agent III consists of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 7.0:1.0:1.0.

[0041] Example 2

[0042] A nutritional regulator based on bioactive substances comprises the following raw materials in parts by weight: 20 parts yeast polysaccharide, 25 parts fulvic acid, 30 parts highly active carbon source, 10 parts compound biological enzyme, 15 parts algal growth factor, 12 parts compound probiotics, 8 parts betaine, 25 parts compound mineral elements, and 10 parts compound trace elements. The high-activity carbon source consists of glucose and sucrose in a mass ratio of 1.0:1.5; the complex bio-enzyme consists of protease, amylase, and cellulase in a mass ratio of 7:4:2, with the protease consisting of neutral protease and alkaline protease in a mass ratio of 8:3; the algal growth factor consists of wakame ferment, pomegranate peel ferment, and seaweed ferment in a mass ratio of 6:5:2; the complex probiotic consists of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 4.5:4.0:3.0; the complex mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate, and sodium silicate in a mass ratio of 12:9:6:5; and the complex trace elements consist of EDTA iron sodium, EDTA manganese, EDTA zinc, and selenomethionine in a mass ratio of 5.5:3.0:2.5:0.3.

[0043] The wakame fermented material was prepared by the following method: Dried wakame seaweed free from pollution and mold was pulverized to 80 mesh to obtain wakame powder; Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum were compounded in a mass ratio of 2.2:2.5:1.2 to obtain compound fermentation agent I; water at 37℃ was added at a material-to-liquid ratio of 1:10 (material-to-liquid ratio in g / mL); then, 1.5 times the mass of compound fermentation agent I with a 6% brown sugar solution was added, and the mixture was activated for 25 minutes to obtain activated compound fermented material I; wakame powder, brown sugar, yeast extract, potassium dihydrogen phosphate, and purified water were mixed in a mass ratio of 15:2:0.5:0.1:90 and stirred evenly to obtain fermented material I; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Add 3% by volume of activated compound fermenting agent I to fermenting material I, stir well, and ferment at 37℃ for 48 hours. For the first 12 hours, stir at 60 rpm, then allow to stand and aerate for 10 minutes every 12 hours. After fermentation, raise the temperature to 85℃ and maintain for 15 minutes, then perform solid-liquid separation. Collect the filtrate and concentrate it to 35% of its original volume at 60℃ to obtain concentrated liquid I. Add 2.0 times its weight of protective agent I to concentrated liquid I, stir well, and then homogenize twice at 40 MPa. Then, perform vacuum belt heating at 75℃ and a vacuum of -0.08 MPa. After drying, pulverize and pass through a 100-mesh sieve to obtain wakame fermented material. Protective agent I consists of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 8.0:2.5:1.2.

[0044] The pomegranate peel ferment is prepared by the following method: Select pollution-free and mold-free dried pomegranate peels, pulverize them to 60 mesh to obtain pomegranate peel powder; combine Bacillus subtilis, Aspergillus niger, and Lactobacillus plantarum in a mass ratio of 3.0:1.1:0.8-1.0 to obtain compound fermentation agent II; add water at 35℃ in a material-to-liquid ratio of 1:10 (material-to-liquid ratio in g / mL); then add 1.5 times the mass of compound fermentation agent II in a 6% brown sugar solution, and activate for 40 minutes to obtain activated compound fermentation agent II; mix pomegranate peel powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 12:3:0.8:0.1:0.05:95, stir evenly, and obtain… Fermentation medium II: 4% (by volume) of activated compound fermentation agent II was added to fermentation medium II, stirred evenly, and fermented at 31℃ for 96 hours. For the first 24 hours, fermentation was carried out with stirring at 60 rpm and aeration for 4 minutes every 6 hours. Subsequent fermentation was allowed to stand. After fermentation, the temperature was raised to 85℃ and maintained for 15 minutes, followed by solid-liquid separation. The filtrate was collected and concentrated to 30% of its original volume at 55℃ to obtain concentrated liquid II. 2.0 times its mass of protective agent II was added to concentrated liquid II, stirred evenly, and then homogenized twice under 30 MPa pressure. Vacuum belt heating was then performed at 70℃ and a vacuum degree of -0.08 MPa. After drying, the mixture was pulverized through a 100-mesh sieve to obtain pomegranate peel fermentation product. Protective agent I consisted of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 7.0:3.0:1.2.

[0045] The *Ulva prolifera* fermented material was prepared by the following method: Dry, unrotten *Ulva prolifera* was selected and pulverized to 100 mesh to obtain *Ulva prolifera* powder; *Bacillus subtilis*, *Bacillus belye*, and *Candida utilis* were compounded in a mass ratio of 2.2:1.6:1.0 to obtain compound fermentation agent III; water at 32℃ was added at a material-to-liquid ratio of 1:10 (material-to-liquid ratio in g / mL); then, 1.5 times the mass of compound fermentation agent III (6% brown sugar solution) was added, and the mixture was activated for 55 minutes to obtain activated compound fermentation agent III; *Ulva prolifera* powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate, and purified water were mixed in a mass ratio of 20:1.5:0.2:0.5:1.0:85 and stirred evenly to obtain fermentation material III; fermentation... Add 5% by volume of activated compound fermentation agent III to material III, stir evenly, and ferment at 29℃ for 72 hours. For the first 48 hours, stir at 60 rpm and aerate for 20 minutes every 8 hours. For the subsequent 48 hours, aerate for 8 minutes every 12 hours while maintaining stirring. After fermentation, raise the temperature to 85℃ and hold for 15 minutes, then perform solid-liquid separation, collect the filtrate, and concentrate it to 40% of its original volume at 58℃ to obtain concentrated liquid III. Add 1.8 times its mass of protective agent III to concentrated liquid III, stir and mix evenly, then homogenize twice at 40 MPa pressure, followed by vacuum belt heating at 68℃ and a vacuum degree of -0.08 MPa. After drying, pulverize and pass through a 100-mesh sieve to obtain the *Ulva prolifera* fermented product. Protective agent III consists of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 8.0:1.5:1.5.

[0046] Example 3

[0047] A nutritional regulator based on bioactive substances comprises the following raw materials in parts by weight: 15 parts yeast polysaccharide, 20 parts fulvic acid, 25 parts highly active carbon source, 8 parts compound biological enzyme, 12 parts algal growth factor, 10 parts compound probiotics, 5 parts betaine, 10 parts compound mineral elements, and 8 parts compound trace elements. The high-activity carbon source consists of glucose and sucrose in a mass ratio of 1.0:1.2; the complex bio-enzyme consists of protease, amylase, and cellulase in a mass ratio of 6:3:1.5, with the protease consisting of neutral protease and alkaline protease in a mass ratio of 7.5:2.5; the algal growth factor consists of wakame ferment, pomegranate peel ferment, and seaweed ferment in a mass ratio of 5:4:1.5; the complex probiotic consists of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 4.0:3.5:2.5; the complex mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate, and sodium silicate in a mass ratio of 10:7.5:5:4; and the complex trace elements consist of EDTA iron sodium, EDTA manganese, EDTA zinc, and selenomethionine in a mass ratio of 5.0:2.5:2.0:0.2.

[0048] The wakame fermented material was prepared by the following method: Dried wakame seaweed free from pollution and mold was pulverized to 70 mesh to obtain wakame powder; Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum were compounded in a mass ratio of 2.0:2.21.0 to obtain compound fermentation agent I; water at 36℃ was added at a material-to-liquid ratio of 1:8 (material-to-liquid ratio in g / mL); then, 1.2 times the mass of compound fermentation agent I with a 5% brown sugar solution was added, and the mixture was activated for 30 minutes to obtain activated compound fermented material I; wakame powder, brown sugar, yeast extract, potassium dihydrogen phosphate, and purified water were mixed in a mass ratio of 12:1.5:0.4:0.1:85 and stirred evenly to obtain fermented material I; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Activated compound fermenter I (2% by volume) was added to fermentation material I, stirred evenly, and fermented at 35℃ for 60 hours. For the first 12 hours, fermentation was carried out with stirring at 50 rpm, followed by static fermentation with aeration for 12 minutes every 12 hours. After fermentation, the temperature was raised to 83℃ and maintained for 18 minutes, then solid-liquid separation was performed. The filtrate was collected and concentrated to 42% of its original volume at 55℃ to obtain concentrated liquid I. Then, 1.8 times its mass of protective agent I was added to concentrated liquid I, stirred evenly, and homogenized twice at 35 MPa. Vacuum belt heating was then performed at 70℃ and a vacuum degree of -0.07 MPa. After drying, the mixture was pulverized through an 80-mesh sieve to obtain the wakame seaweed fermented product. Protective agent I consisted of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 7.0:2.0:1.0.

[0049] The pomegranate peel fermented product is prepared by the following method: Select pollution-free and mold-free dried pomegranate peels, pulverize them to 50 mesh to obtain pomegranate peel powder; combine Bacillus subtilis, Aspergillus niger, and Lactobacillus plantarum in a mass ratio of 2.7:1.0:0.9 to obtain compound fermentation agent II; add water at 32℃ in a material-to-liquid ratio of 1:8 (material-to-liquid ratio in g / mL); then add 1.2 times the mass of compound fermentation agent II in a 5% brown sugar solution, and activate for 45 minutes to obtain activated compound fermentation product II; mix pomegranate peel powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 10:2.5:0.6:0.1:0.05:90, stir evenly, and obtain the fermented material. II; Add 3% by volume of activated compound fermenter II to fermentation material II, stir evenly, and ferment at 30℃ for 108h. For the first 24h, stir fermentation at 50rpm and aerate for 5min every 6h. Then allow static fermentation. After fermentation, raise the temperature to 83℃ and maintain for 18min, then perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 35% of the original volume at 53℃ to obtain concentrated liquid II. Add 1.5-2.0 times its mass of protective agent II to concentrated liquid II, stir and mix evenly, then homogenize twice at 35MPa pressure, then perform vacuum belt heating at 65℃ and vacuum degree -0.07MPa. After drying, pulverize and pass through an 80-mesh sieve to obtain pomegranate peel fermentation material. Among them, protective agent I is composed of maltodextrin, β-cyclodextrin and soluble starch in a mass ratio of 6.0:2.5:1.0.

[0050] The *Ulva prolifera* fermented material was prepared by the following method: Dry, unrotten *Ulva prolifera* was selected and pulverized to 90 mesh to obtain *Ulva prolifera* powder; *Bacillus subtilis*, *Bacillus belye*, and *Candida utilis* were compounded in a mass ratio of 2.0:1.5:0.9 to obtain compound fermentation agent III; water at 31℃ was added at a material-to-liquid ratio of 1:8 (material-to-liquid ratio in g / mL); then, 1.2 times the mass of compound fermentation agent III (5% brown sugar solution) was added, and the mixture was activated for 60 minutes to obtain activated compound fermentation agent III; *Ulva prolifera* powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate, and purified water were mixed in a mass ratio of 18:1.2:0.2:0.4:0.8:80 and stirred evenly to obtain fermentation material III; [The text abruptly ends here, likely due to an incomplete translation or missing information.] Add 4% by volume of activated compound fermentation agent III to ingredient III, stir well, and ferment at 28℃ for 84 hours. For the first 48 hours, stir at 50 rpm and aerate for 25 minutes every 8 hours. For the subsequent 48 hours, aerate for 10 minutes every 12 hours while maintaining stirring. After fermentation, raise the temperature to 83℃ and hold for 18 minutes, then perform solid-liquid separation, collect the filtrate, and concentrate it to 45% of its original volume at 55℃ to obtain concentrated liquid III. Add 1.5 times its mass of protective agent III to concentrated liquid III, stir well, and then homogenize twice at 35 MPa. Then, perform vacuum belt heating at 65℃ and a vacuum of -0.07 MPa. After drying, pulverize and pass through an 80-mesh sieve to obtain the *Ulva prolifera* fermentation product. Protective agent III consists of maltodextrin, β-cyclodextrin, and soluble starch in a mass ratio of 7.5:1.2:1.2.

[0051] The nutrient regulator based on bioactive substances described in Examples 1-3 is prepared by the following method, including the following steps:

[0052] S1. Mix the composite mineral elements, composite trace elements, highly active carbon source, and fulvic acid at 200 rpm for 20 minutes until homogeneous to obtain mixture I.

[0053] S2. Add yeast polysaccharide, compound biological enzyme, algae growth factor and betaine to mixture I, stir at 120 rpm for 25 min, mix evenly to obtain mixture II;

[0054] S3. Add compound probiotics to mixture II, stir at 80 rpm for 12 minutes to mix evenly, and then package to obtain the nutritional regulator.

[0055] Example 4

[0056] Compared with Example 3, the difference in this embodiment is that the nutritional regulator based on bioactive substances is prepared by the following method, including the following steps:

[0057] S1. Mix the composite mineral elements, composite trace elements, highly active carbon source, and fulvic acid at 150 rpm for 25 minutes until homogeneous to obtain mixture I.

[0058] S2. Add yeast polysaccharide, compound biological enzyme, algae growth factor and betaine to mixture I, stir at 80 rpm for 30 min, mix evenly to obtain mixture II;

[0059] S3. Add compound probiotics to mixture II, stir at 60 rpm for 15 minutes until well mixed, and then package to obtain the nutritional regulator.

[0060] Example 5

[0061] Compared with Example 3, the difference in this embodiment is that the nutritional regulator based on bioactive substances is prepared by the following method, including the following steps:

[0062] S1. Mix the composite mineral elements, composite trace elements, highly active carbon source, and fulvic acid at 250 rpm for 15 minutes until homogeneous to obtain mixture I.

[0063] S2. Add yeast polysaccharide, compound biological enzyme, algae growth factor and betaine to mixture I, stir at 150 rpm for 20 min, mix evenly to obtain mixture II;

[0064] S3. Add compound probiotics to mixture II, stir at 100 rpm for 8 minutes until well mixed, and then package to obtain the nutritional regulator.

[0065] Comparative Example 1

[0066] The difference between this comparative example and Example 3 is that the raw materials do not contain algal growth hormones.

[0067] Comparative Example 2

[0068] Compared with Example 3, this comparative example differs in that the algae growth factor does not contain wakame ferment, but is composed of pomegranate peel ferment and seaweed ferment in a mass ratio of 4:1.5.

[0069] Comparative Example 3

[0070] Compared with Example 3, this comparative example differs in that the algae growth factor does not contain pomegranate peel fermentation product, but is composed of wakame fermentation product and sea lettuce fermentation product in a mass ratio of 5:1.5.

[0071] Comparative Example 4

[0072] Compared with Example 3, the difference in this comparative example is that the algae growth factor does not contain Ulva lactuca ferment, but is composed of wakame ferment and pomegranate peel ferment in a mass ratio of 5:4.

[0073] Comparative Example 5

[0074] The difference between this comparative example and Example 3 is that a commercially available compound fertilizer (N-P2O5-K2O = 15-15-15) was used instead of the nutrient regulator.

[0075] Comparative Example 6

[0076] The difference between this comparative example and Example 3 is that copper sulfate was used instead of the nutrient regulator.

[0077] Experiment 1. Effect of fertilization at room temperature (water temperature 25-30℃)

[0078] Thirty similar marine aquaculture ponds (each with an area of ​​1 mu and a water depth of 1.5 meters) were selected in Chengmai County, Hainan Province. Nutrient regulators from Examples 1-5 and Comparative Examples 1-4, and commercially available compound fertilizer from Comparative Example 5 were applied to each pond. Each group had three replicates, and the experimental period was 21 days. Examples 1-5 and Comparative Examples 1-5 were applied at 1.5 kg / mu to the entire pond, while Comparative Example 5 was applied at 2.0 kg / mu. Algal biomass and algal composition were measured at the beginning of the experiment, on day 7, and at the end of the experiment. The average values ​​for each group were recorded in Table 1.

[0079] Table 1

[0080]

[0081] As shown in Table 1, the algal biomass of Comparative Example 5 peaked on day 7 and then rapidly declined, reaching a level comparable to the initial value by day 21. However, after applying the nutrient regulators of Examples 1-5 of this invention, the algal biomass remained at approximately 19.0 mg / L on day 21, significantly higher than the initial value and that of Comparative Example 5. Furthermore, Table 1 shows that after applying the nutrient regulators of this invention, the proportion of diatoms in Examples 1-5 significantly increased, becoming the dominant species. The wakame ferment in the nutrient regulators specifically promotes diatom proliferation, the pomegranate peel ferment inhibits the growth of harmful algae, and the Ulva ferment promotes the growth of green algae. The synergistic effect of these three components produces a better optimization of the algal community composition.

[0082] Experiment 2. Effect of controlling harmful algae (during cyanobacterial blooms)

[0083] Three similar freshwater aquaculture ponds, each 1 mu in area and 1.5 meters deep, located in Chengmai County, Hainan Province, were selected during a cyanobacterial bloom. The ponds were randomly divided into three groups, with one pond in each group. The nutrient regulator from Example 3 and copper sulfate from Comparative Example 6 were applied to each group, along with a blank control group receiving no treatment. The nutrient regulator was applied at a rate of 2.0 kg / mu to the entire pond, and copper sulfate at a rate of 0.5 kg / mu to the entire pond. Cyanobacterial density and the percentage of beneficial algae (diatoms + green algae) were recorded at the beginning of the experiment and on days 3, 7, and 14. The results are shown in Table 2.

[0084] Table 2

[0085]

[0086] As can be seen from Table 2, Example 3, which applied the nutrient regulator of the present invention, had a significant inhibitory effect on cyanobacteria, while promoting the growth of diatoms and green algae, thereby increasing the proportion of beneficial algae.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A nutrient regulator based on bioactive substances, characterized in that, The ingredients include the following parts by weight: 10-20 parts yeast polysaccharide, 15-25 parts fulvic acid, 20-30 parts highly active carbon source, 5-10 parts compound biological enzyme, 8-15 parts algae growth factor, 8-12 parts compound probiotics, 3-8 parts betaine, 15-25 parts compound mineral elements, and 5-10 parts compound trace elements. The algae growth factor is composed of fermented wakame seaweed, fermented pomegranate peel, and fermented seaweed in a mass ratio of 4-6:3-5:1-2.

2. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The highly active carbon source is composed of glucose and sucrose in a mass ratio of 1.0:1.0-1.

5.

3. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The complex bioenzyme is composed of protease, amylase, and cellulase in a mass ratio of 5-7:2-4:1-2, and the protease is composed of neutral protease and alkaline protease in a mass ratio of 7-8:2-3.

4. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The wakame fermented product is prepared by the following method: Select pollution-free and mold-free dried wakame and pulverize it to 60-80 mesh to obtain wakame powder; Combine Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum in a mass ratio of 1.8-2.2:2.0-2.5:0.8-1.2 to obtain compound fermentation agent I; add water at 35-37℃ at a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL); then add 1.0-1.5 times the mass of compound fermentation agent I with a 4-6% concentration of brown sugar solution, and activate for 25-35 minutes to obtain activated compound fermented product I; mix wakame powder, brown sugar, yeast extract, potassium dihydrogen phosphate, and purified water in a mass ratio of 10-15:1-2:0.3-0.5:0.1:80-90, and stir evenly to obtain fermented material I; add 1-3% by volume of activated compound fermented product I to fermented material I, stir evenly, and heat at 33℃... Ferment at -37℃ for 48-72 hours, stirring at 40-60 rpm for the first 12 hours, followed by static fermentation with aeration for 10-15 minutes every 12 hours. After fermentation, raise the temperature to 80-85℃ and maintain it for 15-20 minutes, then perform solid-liquid separation, collect the filtrate, and concentrate it to 35-50% of its original volume at 50-60℃ to obtain concentrate I. Then, add 1.5-2.0 times its weight of a protective agent to concentrate I. I. Stir and mix evenly, then homogenize 2-3 times under a pressure of 30-40 MPa, followed by vacuum belt heating with a heating plate temperature of 65-75℃ and a vacuum degree of -0.06 to -0.08 MPa. After drying, pulverize through a 60-100 mesh sieve to obtain the wakame fermented product; the protective agent I is composed of maltodextrin, β-cyclodextrin and soluble starch in a mass ratio of 6.0-8.0:1.5-2.5:0.8-1.

2.

5. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The pomegranate peel ferment is prepared by the following method: Select pollution-free and mold-free dried pomegranate peels, pulverize them to 40-60 mesh to obtain pomegranate peel powder; combine Bacillus subtilis, Aspergillus niger, and Lactobacillus plantarum in a mass ratio of 2.5-3:0.9-1.1:0.8-1.0 to obtain compound fermentation agent II; add water at 30-35℃ at a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL), and then add the compound fermentation agent II by mass... Activate with 1.0-1.5 times the concentration of 4-6% brown sugar solution for 40-50 minutes to obtain activated compound fermentation material II; mix pomegranate peel powder, brown sugar, yeast extract, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 8-12:2-3:0.5-0.8:0.1:0.05:85-95, and stir evenly to obtain fermentation material II; add 2-4% of its volume of activated compound fermentation material II to fermentation material II, stir evenly, and ferment at 29-31℃ for 96-120 hours. For the first 24 hours, stir fermentation at 40-60 rpm and aerate for 4-6 minutes every 6 hours. Then allow to stand for fermentation. After fermentation, raise the temperature to 80-85℃ and maintain it for 15-20 minutes, then perform solid-liquid separation, collect the filtrate, and concentrate the filtrate to 30-40% of its original volume at 50-55℃ to obtain concentrated liquid II; add 1.5-2.0 times its mass of protective agent to concentrated liquid II. Agent II is stirred and mixed evenly, then homogenized 2-3 times under a pressure of 30-40 MPa, followed by vacuum belt heating at a temperature of 60-70℃ and a vacuum degree of -0.06 to -0.08 MPa. After drying, it is pulverized through a 60-100 mesh sieve to obtain pomegranate peel fermentation product; the protective agent I is composed of maltodextrin, β-cyclodextrin and soluble starch in a mass ratio of 5.0-7.0:2.0-3.0:0.8-1.

2.

6. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The *Ulva prolifera* fermented product is prepared by the following method: Dry, unrotten *Ulva prolifera* is selected and pulverized to 80-100 mesh to obtain *Ulva prolifera* powder; *Bacillus subtilis*, *Bacillus belye*, and *Candida utilis* are compounded at a mass ratio of 1.8-2.2:1.4-1.6:0.8-1.0 to obtain compound fermentation agent III; water at a temperature of 30-32℃ is added at a material-to-liquid ratio of 1:6-10 (material-to-liquid ratio unit is g / mL); then, 1 part by mass of compound fermentation agent III is added. Activate with a 4-6% brown sugar solution (0.0-1.5 times the volume) for 55-65 minutes to obtain activated compound fermentation material III; mix seaweed powder, brown sugar, potassium dihydrogen phosphate, ammonium sulfate, and purified water in a mass ratio of 15-20:1.0-1.5:0.2:0.3-0.5:0.5-1.0:75-85, and stir evenly to obtain fermentation material III; add 3-5% by volume of activated compound fermentation material III to fermentation material III, stir evenly, and ferment at 27°C. Fermentation was carried out at -29℃ for 72-96 hours. For the first 48 hours, fermentation was carried out with stirring at 40-60 rpm and aeration for 20-30 minutes every 8 hours. Afterward, aeration was carried out for 8-12 minutes every 12 hours while maintaining stirring. After fermentation, the temperature was raised to 80-85℃ and maintained for 15-20 minutes. Solid-liquid separation was then performed, and the filtrate was collected. The filtrate was concentrated to 40-50% of its original volume at 52-58℃ to obtain concentrated solution III. 1.2-1% of the filtrate was added to concentrated solution III. The protective agent III was mixed at a ratio of 0.8:1.0-1.5:1.0-1.5 by stirring until homogeneous. Then, it was homogenized 2-3 times under a pressure of 30-40 MPa. After that, it was subjected to vacuum belt heating with a heating plate temperature of 62-68℃ and a vacuum degree of -0.06 to -0.08 MPa. After drying, it was pulverized through a 60-100 mesh sieve to obtain the fermented seaweed. The protective agent III was composed of maltodextrin, β-cyclodextrin and soluble starch in a mass ratio of 7.0-8.0:1.0-1.5:1.0-1.

5.

7. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The compound probiotics consist of Bacillus subtilis, Bacillus licheniformis, and photosynthetic bacteria in a mass ratio of 3.5-4.5:3.0-4.0:2.0-3.

0.

8. A nutritional regulator based on bioactive substances as described in claim 1, characterized in that, The composite mineral elements consist of urea, potassium dihydrogen phosphate, potassium sulfate, and sodium silicate in a mass ratio of 8-12:6-9:4-6:3-5, and the composite trace elements consist of EDTA iron sodium, EDTA manganese, EDTA zinc, and selenomethionine in a mass ratio of 4.5-5.5:2.0-3.0:1.5-2.5:0.1-0.

3.

9. The method for preparing a nutrient regulator based on bioactive substances as described in claim 1, characterized in that, Includes the following steps: S1. Mix the composite mineral elements, composite trace elements, highly active carbon source, and fulvic acid evenly to obtain mixture I; S2. Add yeast polysaccharide, compound biological enzyme, algae growth factor and betaine to mixture I, stir and mix evenly to obtain mixture II; S3. Add compound probiotics to mixture II, stir and mix evenly, then package to obtain a nutritional regulator.

10. The method for preparing a nutrient regulator based on bioactive substances as described in claim 9, characterized in that, In S1, the stirring speed is 150-250 rpm and the stirring time is 15-25 min; in S2, the stirring speed is 80-150 rpm and the stirring time is 20-30 min; and in S3, the stirring speed is 60-100 rpm and the stirring time is 8-15 min.

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