Ammonia assimilation arthrobacter agallinarum and application thereof in aquaculture water treatment
By using the ammonia-assimilating Arthrobacter aa01 strain for ammonia assimilation and denitrification under aerobic conditions, the problem of nitrogen pollution in aquaculture wastewater was solved, achieving efficient and low-cost denitrification and adapting to complex aquaculture environments.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HEILONGJIANG UNIV
- Filing Date
- 2026-03-23
- Publication Date
- 2026-05-12
AI Technical Summary
Existing aquaculture wastewater treatment technologies are ineffective at removing nitrogen pollution, especially in the presence of high salt and antibiotics where denitrification efficiency is unstable. Traditional microbial agents lack salt tolerance and antibiotic adaptability, leading to secondary pollution risks and high treatment costs.
The ammonia-assimilating Arthrobacter arilaitensis strain Aa01 was used to achieve efficient ammonia assimilation and denitrification by using NH4+-N, NO2--N and NO3--N as electron acceptors for respiration under aerobic conditions. It is adapted to high-salt and slightly alkaline environments, resistant to acid and alkali fluctuations, and can simultaneously remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen.
It significantly improves denitrification efficiency, shortens start-up cycle, reduces energy consumption, simplifies process flow, reduces costs, adapts to various carbon and nitrogen sources, has a wide range of applications, and is salt tolerant, making it suitable for the treatment of seawater and low-salinity aquaculture wastewater.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of wastewater treatment technology, and in particular to an ammonia-assimilating Arthrobacter bacillus and its application in aquaculture water treatment. Background Technology
[0002] my country's aquaculture industry has maintained a sustained growth trend in recent years, becoming one of the core industries for ensuring food security and the supply of high-quality protein. According to statistics, from January to November 2025, my country's total aquatic product output reached 66.4887 million tons, a year-on-year increase of 4.05%. Of this, marine aquaculture output was 24.7929 million tons, and freshwater aquaculture output was 31.7995 million tons, achieving year-on-year growth of 5.23% and 4.21%, respectively. Along with the expansion of aquaculture scale and the increase in intensification, the discharge of aquaculture wastewater has also shown a synchronous growth trend, necessitating strengthened treatment of aquaculture wastewater while ensuring the supply of aquatic products.
[0003] Aquaculture wastewater presents significant challenges, including complex pollutant composition, high nutrient concentrations, large salinity fluctuations, and antibiotic residues, with nitrogen pollution being particularly severe. Excessive nitrogen accumulation in water bodies not only disrupts the ecological balance and harms the health of aquatic animals but may also pose a serious threat to human health. Therefore, nitrogen removal from aquaculture wastewater has become a crucial task for ensuring the high-quality development of the aquaculture industry. During the aquaculture process, approximately 65% of the nitrogen in feed is not effectively utilized by farmed organisms and enters the water as waste, transforming into ammonia nitrogen. Furthermore, the nitrite produced from ammonia nitrogen conversion can combine with ammonia compounds to form carcinogens, which can enter the human body through the consumption of aquatic products, leading to health risks such as methemoglobinemia. Simultaneously, nitrogen compounds accelerate eutrophication, induce algal blooms, and further deteriorate the aquaculture ecological environment.
[0004] Currently, aquaculture wastewater treatment technologies mainly include physical treatment, chemical treatment, biological treatment, and combined processes, but all have significant limitations: physical treatment (such as filtration and sedimentation) can only remove suspended particulate matter and cannot effectively degrade dissolved ammonia nitrogen, and its operating costs are high; chemical treatment (such as adding oxidants and flocculants) is prone to secondary pollution, disrupting the microecological balance of the water body, and may also lead to excessive drug residues in aquatic products; traditional biological treatment technologies (such as constructed wetlands and anaerobic reactors) are significantly limited by environmental conditions such as hydraulic retention time and temperature, resulting in unstable nitrogen removal efficiency and poor adaptability to aquaculture wastewater with high salinity and antibiotic residues. Existing commercial microbial agents are mostly nitrifying-denitrifying bacteria, which lack sufficient tolerance to salinity and antibiotics in aquaculture environments, making it difficult to maintain effective activity in seawater aquaculture or ponds treated with drugs, leading to a significant reduction in nitrogen removal efficiency. Therefore, developing highly efficient denitrifying bacterial agents adapted to the complex environment of aquaculture has become a key requirement for technological breakthroughs in the industry. Screening ammonia-assimilating bacteria with salt and antibiotic resistance characteristics, which directly convert ammonia nitrogen into their own biomass through metabolism, and realize the resource utilization of pollutants, can not only avoid the secondary pollution risks of traditional technologies, but also adapt to the extreme environment of high-density aquaculture. This is the core breakthrough for solving the problem of water pollution in aquaculture and promoting the green transformation of the industry. Summary of the Invention
[0005] This invention provides an ammonia-assimilating Arthrobacter bacillus and its application in aquaculture water treatment.
[0006] The ammonia-assimilated Arthrobacter arilaitensis of this invention is Arthrobacter arilaitensis Aa01, deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with accession number CGMCC No. 36829 and deposit date of December 1, 2025.
[0007] Furthermore, the culture conditions for Arthrobacter arilaitensis Aa01 were: cultured at 28-32℃ in a constant temperature shaking bed at 120-160 r / min for 22-26 h.
[0008] The application of the ammonia-assimilating Arthrobacter spp. described in this invention in aquaculture water treatment.
[0009] Furthermore, the ammonia-assimilating Arthrobacter suspension was added to the aquaculture wastewater for treatment.
[0010] Furthermore, the OD600 value of the ammonia-assimilated Arthrobacter bacillus suspension is 0.9-1.1.
[0011] Furthermore, the treatment is carried out by stirring or oscillating at 30°C.
[0012] Furthermore, the ammonia-assimilating Arthrobacter bacillus suspension is added at a ratio of 2% to 3% of the volume of aquaculture wastewater.
[0013] The ammonia-assimilating Arthrobacter aa01 provided by this invention is a microbial strain with significant characteristics and application value. The most significant feature of this strain is its unique nitrogen transformation mechanism, which enables it to efficiently convert NH4+ under aerobic conditions. + -N, NO2 - -N and NO3 - -N acts as an electron acceptor in respiration, breaking through the dependence of traditional denitrification processes on anaerobic environments. Compared with conventional biological denitrification bacteria, the Arthrobacter arilaitensis Aa01 strain exhibits superior performance advantages: its rapid growth and metabolic rate allows it to quickly accumulate biomass using substrates such as ammonia nitrogen and organic matter; it rapidly enters the logarithmic growth phase after inoculation, significantly shortening the system start-up period; its fast substrate utilization rate and high metabolic activity enable it to quickly form a dominant bacterial community, improving system stability and shock resistance; this strain has strong environmental stress tolerance, adapting to high-salt and slightly alkaline environments, significantly reducing process control energy consumption; it is also resistant to acid-base fluctuations, eliminating the need for frequent pH adjustments; it can utilize multiple carbon and nitrogen sources; it has low substrate selectivity and wide applicability; under aerobic conditions, it can simultaneously remove ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen; denitrification can be completed in a single pond; the process is simple; and it can operate stably in neutral to slightly alkaline environments, adapting to the pH of fish and shrimp aquaculture water. Highly compatible with salt tolerance, it can be adapted to the treatment of marine aquaculture and low-salinity aquaculture wastewater, and has strong versatility across water areas. The greatest innovation of Arthrobacter aa01 provided by this invention is that it can achieve efficient denitrification through ammonia assimilation under high salinity and in the presence of antibiotics. This special metabolic pathway not only greatly simplifies the traditional multi-stage biological denitrification process, but also significantly reduces treatment costs and energy consumption. Attached Figure Description
[0014] Figure 1 This is a scanning electron microscope image of strain Aa01.
[0015] Figure 2 Phylogenetic tree of strain Aa01;
[0016] Figure 3 The image shows the removal effect of Arthrobacter arilaitensis Aa01 on ammonia nitrogen.
[0017] Figure 4 The effect of Arthrobacter arilaitensis Aa01 on ammonia nitrogen removal under different C / N ratios was investigated.
[0018] Figure 5 The effect of Arthrobacter arilaitensis Aa01 on ammonia nitrogen removal under different carbon sources;
[0019] Figure 6 The effect of Arthrobacter arilaitensis Aa01 on the removal of ammonia nitrogen at different concentrations was studied.
[0020] Figure 7 The denitrification effect of Arthrobacter arilaitensis Aa01 under different concentrations of NaCl;
[0021] Figure 8 The denitrification effect of Arthrobacter arilaitensis Aa01 under mixed nitrogen source;
[0022] Figure 9 The denitrification effect of Arthrobacter arilaitensis Aa01 under different concentrations of oxytetracycline;
[0023] Figure 10 The effect of Arthrobacter arilaitensis Aa01 on denitrification of actual aquaculture wastewater. Detailed Implementation
[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0025] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other.
[0026] Example 1: Screening of Arthrobacter oryzae that assimilates ammonia according to the present invention
[0027] 1. Culture medium
[0028] Nitrification medium: 1.5 g / L K2HPO4, 0.5 g / L KH2PO4, 1.0 g / L NaCl, 0.1 g / L MgSO4·7H2O, 0.365 g / L NH4Cl, 3.42 g / L CH3COONa and 0.2% (v / v) trace element solution, pH=7.
[0029] The trace element solution contained 0.5 g / L ZnSO4, 1.0 g / L CaCl2, 0.5 g / L MnCl2·4H2O, 0.5 g / L FeSO4·7H2O, and 0.2 g / L CoCl2·6H2O. The culture medium was sterilized at 121℃ and 0.11 MPa for 30 minutes.
[0030] LB medium: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, pH=7.
[0031] Oxytetracycline (OTC) was of analytical grade and purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Aquaculture sediment was collected from Harbin City, Heilongjiang Province.
[0032] 2. Screening Process
[0033] 2.1 Enrichment
[0034] Mix 100 ml of aquaculture sediment with 200 ml of nitrification medium (adjusted to 1% (v / v) by adding NaCl), place the mixture in a 0.5 L Erlenmeyer flask, and incubate at 20 °C and 140 rpm in a constant temperature shaker for 4 days.
[0035] 2.2 Domestication
[0036] After 4 days of enrichment culture, 100 ml of the mud-water mixture of bottom sediment and culture medium was transferred to a new 200 ml nitrification medium with the salt concentration adjusted to 3% (v / v) by adding NaCl, and placed in a constant temperature shaker (20℃, 140 rpm) for acclimatization culture. After 4 days of culture, 100 ml of the suspension was transferred to a new 0.5 L Erlenmeyer flask containing 0.2 L of newly prepared nitrification medium with the salt concentration adjusted to 5% (v / v) by adding NaCl, and placed in the same aerobic conditions for culture. Subsequent inoculations were then carried out in new, identical culture media, with no further changes in salinity.
[0037] 2.4 Screening
[0038] After 16 days of culture, the suspension was transferred, serially diluted, streaked on LB agar plates, and cultured in a constant temperature incubator (30℃). After the colonies grew, single colonies were picked up with an inoculation loop in a clean bench and placed on sterile enrichment medium LB agar plates for repeated streaking. After repeated streaking purification, single colonies were stored for later use.
[0039] 2.5 Detection of single colonies
[0040] 2.5.1 Scanning electron microscope image
[0041] The isolated and purified single bacterial strains were numbered. Strain Aa01 was inoculated into enrichment medium, activated, and cultured to the logarithmic growth phase. Fresh cells were collected and pretreated with PBS washing, glutaraldehyde fixation, ethanol gradient dehydration, isoamyl acetate replacement, and freeze-drying. Morphological observation of the strain was then performed using scanning electron microscopy (SEM). The results are as follows: Figure 1 As shown ( Figure 1 (Scanning electron micrograph of strain Aa01), from Figure 1 As can be seen from the data, the strain Aa01 obtained by screening in this invention is a bacillus.
[0042] 2.5.2 Phylogenetic tree was constructed based on 16S rRNA phylogenetic analysis. Figure 2 This is a phylogenetic tree for strain Aa01. Strain Aa01 was identified as *Arthrobacter arilaitensis*.
[0043] 2.5.3 Gram staining showed that strain Aa01 was a Gram-positive bacterium with a diameter of 400-600 nm and a length of 500-700 nm.
[0044] The strain Aa01 was named Arthrobacter arilaitensis Aa01 and was deposited on December 1, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36829.
[0045] Example 2: Identification of the ability of Arthrobacter albopictus to assimilate ammonia according to the present invention
[0046] Culture medium:
[0047] Nitrification medium: 1.5 g / L K2HPO4, 0.5 g / L KH2PO4, 1.0 g / L NaCl, 0.1 g / L MgSO4·7H2O, 0.365 g / L NH4Cl, 3.42 g / L CH3COONa and 0.2% (v / v) trace element solution, pH=7.
[0048] The trace element solution contained 0.5 g / L ZnSO4, 1.0 g / L CaCl2, 0.5 g / L MnCl2·4H2O, 0.5 g / L FeSO4·7H2O, and 0.2 g / L CoCl2·6H2O. The culture medium was sterilized at 121℃ and 0.11 MPa for 30 minutes.
[0049] LB medium: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, pH=7.
[0050] 1. Preparation of bacterial suspension
[0051] The strain Arthrobacter arilaitensis Aa01 was inoculated into 100 mL of sterilized LB medium using an inoculation loop and placed in a constant temperature shaking bed. It was cultured at 140 r / min and 30 °C for 24 h. The bacterial cells were collected by centrifugation, diluted with sterile water to an OD600 value of 1.0, and mixed well to obtain the bacterial solution for subsequent experiments.
[0052] 2. Ammonia nitrogen removal efficiency
[0053] The bacterial suspension was inoculated into nitrification medium at 2.5% (v / v), and the C / N ratio was controlled to be 15 by adjusting the amount of sodium acetate. The experiment was conducted in a constant-temperature shaker at 30℃ and 140rpm, with OD600 and NH4+ levels in the water measured periodically. + -N, NO3 - -N, NO2 - Changes in the concentration of water quality indicators such as -N. Figure 3 The image shows the removal effect of Arthrobacter arilaitensis Aa01 on ammonia nitrogen.
[0054] Indicates inorganic nitrogen. Indicates organic nitrogen, Indicates NH4 + , It means NO3 - , NO2 - , Indicates COD, Indicates OD600. From Figure 3 It can be seen that the average removal rate of ammonia nitrogen can reach 93.56%, and it is converted into organic nitrogen through assimilation.
[0055] 3. Ammonia nitrogen removal rate under different C / N ratios
[0056] The bacterial suspension was inoculated into nitrification medium at a concentration of 2.5% (v / v). The C / N ratio was controlled by varying the amount of sodium acetate at 1, 3, 5, 7, 10, and 15. The experiment was conducted in a constant-temperature shaker at 30℃ and 140 rpm, and the OD600 and NH4+ levels in the water were measured periodically. + Changes in the concentration of water quality indicators such as -N. Figure 4 The figure shows the removal efficiency of ammonia nitrogen by Arthrobacter arilaitensis Aa01 under different C / N ratios.
[0057] Indicates NH4 + (C / N=1) Indicates NH4 + (C / N=3) Indicates NH4 + (C / N=5) Indicates NH4 + (C / N=7) Indicates NH4 + (C / N=10) Indicates NH4 + (C / N=15); Indicates OD600 (C / N=1), Indicates OD600 (C / N=3), Indicates OD600 (C / N=5), Indicates OD600 (C / N=7), Indicates OD600 (C / N=10). This indicates OD600 (C / N=15); Indicates COD (C / N=1), Indicates COD (C / N=3), Indicates COD (C / N=5), This indicates COD (C / N=7). Indicates COD (C / N=10). This indicates COD (C / N=15). From Figure 4It can be seen that the higher the C / N ratio, the higher the ammonia nitrogen removal rate of Arthrobacter arilaitensis Aa01; the ammonia nitrogen removal rates reached 0.00%, 8.06%, 50.93%, 68.99%, and 74.08% when C / N = 1, 3, 5, 7, and 10, respectively. The average removal rate reached 93.56% when C / N = 15.
[0058] 4. Research on nitrogen removal from different carbon sources
[0059] The bacterial suspension was inoculated into nitrification medium at a concentration of 2.5%. Experiments were conducted by varying the carbon source, with the C / N ratio controlled to be 15 by adjusting the amount of carbon source. The experiments were carried out in a constant-temperature shaker at 30℃ and 140rpm, with OD600 and NH4+ levels in the water measured periodically. + Changes in the concentration of water quality indicators such as -N. Figure 5 The figure shows the removal efficiency of ammonia nitrogen by Arthrobacter arilaitensis Aa01 under different carbon sources.
[0060] Indicates NH4 + (sucrose), Indicates NH4 + (Sodium citrate) Indicates NH4 + (glucose), Indicates NH4 + (Potassium sodium tartrate) Indicates NH4 + (Sodium acetate); Indicates OD600 (sucrose). Indicates OD600 (sodium citrate), Indicates OD600 (glucose). Indicates OD600 (potassium sodium tartrate). This indicates OD600 (sodium acetate).
[0061] from Figure 5 It can be seen that the optimal carbon source for Arthrobacter arilaitensis Aa01 is sodium acetate, and when sodium acetate is used as the carbon source, the average removal rate of ammonia nitrogen can reach 93.56%.
[0062] 5. Denitrification capacity under different concentrations of ammonia nitrogen
[0063] The bacterial suspension was inoculated into the nitrification medium at a concentration of 2.5% (v / v). The ammonia nitrogen concentration was controlled by varying the amount of NH4Cl at 25, 50, 100, 200, and 300 mg / L. The experiment was conducted in a constant-temperature shaker at 30℃ and 140 rpm, and the OD600 and NH4Cl concentrations in the water were measured periodically. + Changes in the concentration of water quality indicators such as -N. Figure 6 The figure shows the removal efficiency of Arthrobacter arilaitensis Aa01 for different concentrations of ammonia nitrogen.
[0064] Indicates NH4 + (NH4) + =25mg / L), Indicates NH4 + (NH4) + =50mg / L), Indicates NH4 + (NH4) + =100mg / L), Indicates NH4 + (NH4) + =200mg / L), Indicates NH4 + (NH4) + =300mg / L); Indicates OD600 (NH4) + =25mg / L), Indicates OD600 (NH4) + =50mg / L), Indicates OD600 (NH4) + =100mg / L), Indicates OD600 (NH4) + =200mg / L), Indicates OD600 (NH4) + =300mg / L); It represents COD (NH4) + =25mg / L), It represents COD (NH4) + =50mg / L), It represents COD (NH4) + =100mg / L), It represents COD (NH4) + =200mg / L), It represents COD (NH4) + =300 mg / L). From Figure 6It can be seen that Arthrobacter arilaitensis Aa01 can efficiently remove ammonia nitrogen at a maximum concentration of 100 mg / L, with an average removal rate of 93.56%, which has potential advantages for the treatment of aquaculture wastewater.
[0065] 6. Denitrification capacity at different salt concentrations
[0066] The bacterial suspension was inoculated into the nitrification medium at a concentration of 2.5% (v / v). The C / N ratio was controlled at 15 by varying the amount of sodium acetate, and the NaCl concentration was controlled at 0%, 2.5%, 3.5%, 5%, and 10% by varying the amount of NaCl. The experiment was conducted in a constant temperature shaker at 30℃ and 140 rpm, and the OD600 and NH4+ levels in the water were measured periodically. + Changes in the concentrations of water quality indicators such as nitrogen and COD. Figure 7 The figure shows the denitrification effect of Arthrobacter arilaitensis Aa01 under different concentrations of NaCl.
[0067] Indicates NH4 + (0%) Indicates NH4 + (2.50%) Indicates NH4 + (3.50%) Indicates NH4 + (5%) Indicates NH4 + (10%) Indicates OD600 (0%) This indicates OD600 (2.50%). This indicates OD600 (3.50%). Indicates OD600 (5%) This indicates OD600 (10%). From Figure 7 It can be seen that Arthrobacter arilaitensis Aa01 can still perform ammonia assimilation under the influence of a salt concentration of 3.5%, with an ammonia nitrogen removal rate of up to 92.71%. The results indicate that Arthrobacter arilaitensis Aa01 can normally perform ammonia nitrogen assimilation under certain salt concentration conditions, and has potential advantages in the treatment of aquaculture wastewater.
[0068] 7. Denitrification capacity assessment of mixed nitrogen sources
[0069] The bacterial suspension was inoculated into nitrification medium at 2.5% (v / v). The concentrations of ammonia nitrogen and nitrate nitrogen were controlled to be 50 mg / L by varying the concentrations of NH4Cl and NaNO3. The C / N ratio was controlled to be 15 by adjusting the amount of sodium acetate. The experiment was conducted in a constant-temperature shaker at 30℃ and 140 rpm, and the OD600 and NH4Cl concentrations in the water were measured periodically. + Changes in the concentrations of water quality indicators such as nitrogen and COD. Figure 8 The figure shows the denitrification effect of Arthrobacter arilaitensis Aa01 under a mixed nitrogen source.
[0070] Indicates inorganic nitrogen. Indicates organic nitrogen, Indicates NH4 + , It means NO3 - , NO2 - , Indicates COD, Indicates OD600. From Figure 8 It can be seen that Arthrobacter alaitensis Aa01 can efficiently remove ammonia nitrogen and nitrate nitrogen from water, with a removal rate of 100% for both ammonia nitrogen and nitrate nitrogen.
[0071] 8. Identification of ammonia assimilation capacity at different concentrations of oxytetracycline
[0072] The bacterial suspension was inoculated into nitrification medium at 2.5% (v / v), and the concentration of oxytetracycline in the medium was successively adjusted to 0, 0.5, 2, 4, and 8 mg / L. The C / N ratio was controlled at 15 by varying the amount of sodium acetate. The experiment was conducted in a constant temperature shaker at 30℃ and 140 rpm, and the OD600 and NH4+ levels in the water were measured periodically. + Changes in the concentrations of water quality indicators such as nitrogen and COD. Figure 9 The denitrification effect of Arthrobacter arilaitensis Aa01 at different concentrations of oxytetracycline is shown in the figure. (a) 0 mg / L, (b) 0.5 mg / L, (c) 2 mg / L, (d) 4 mg / L, and (e) 10 mg / L.
[0073] Indicates inorganic nitrogen. Indicates organic nitrogen, Indicates NH4 + , It means NO3 - , NO2 - , Indicates COD, Indicates OD600. From Figure 9 It can be seen that Arthrobacter arilaitensis Aa01 can still perform ammonia assimilation under the influence of oxytetracycline concentrations of 0-8 mg / L, with ammonia nitrogen removal rate reaching up to 100%. The results indicate that Arthrobacter arilaitensis Aa01 can normally perform ammonia assimilation at certain concentrations of oxytetracycline, showing potential advantages in the treatment of aquaculture wastewater.
[0074] 9. Identification of ammonia assimilation capacity in actual aquaculture wastewater
[0075] The bacterial suspension was inoculated into aquaculture wastewater at a concentration of 2.5% (v / v). Initially, only ammonia nitrogen and COD were detected in the water, at 36.38 mg / L and 34.14 mg / L respectively; nitrate nitrogen and nitrite nitrogen were not detected. The experiment was conducted in a constant-temperature shaker at 30℃ and 140 rpm, and NH4+ levels in the water were measured periodically. + -N, NO3 - -N, NO2 - Changes in the concentration of water quality indicators such as -N.
[0076] Figure 10 The figure shows the nitrogen removal effect of Arthrobacter arilaitensis Aa01 in actual aquaculture wastewater, with (a) the Aa01-treated experimental group and (b) the blank control group.
[0077] Indicates NH4 + , It means NO3 - , NO2 - , Indicates COD. From Figure 10 It can be seen that after the addition of Arthrobacter arilaitensis Aa01, ammonia nitrogen in the water was rapidly assimilated and removed within 36 hours, resulting in a removal rate of 99.4% for ammonia nitrogen in aquaculture wastewater. The removal efficiency and overall removal rate were much greater than those of the control group, which greatly improved the treatment of actual aquaculture wastewater.
Claims
1. An ammonia-assimilating Arthrobacter bacillus, characterized in that, The ammonia-assimilating Arthrobacter arilaitensis is named Arthrobacter arilaitensis Aa01, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36829 and deposit date of December 1, 2025.
2. The ammonia-assimilating Arthrobacterium according to claim 1, characterized in that, Arthrobacter arilaitensis Aa01 was cultured at 28-32℃ in a constant temperature shaking bed at 120-160 rpm for 22-26 hours.
3. The application of the ammonia-assimilating Argentobacterium as described in claim 1 in aquaculture water treatment.
4. The application according to claim 3, characterized in that, Ammonia-assimilating Arthrobacter suspension was added to aquaculture wastewater for treatment.
5. The application according to claim 3, characterized in that, The OD600 value of the ammonia-assimilated Arthrobacter bacillus suspension was 0.9-1.
1.
6. The application according to claim 3, characterized in that, The treatment is carried out by stirring or shaking at 30°C.
7. The application according to claim 3, characterized in that, The ammonia-assimilating Arthrobacter bacillus suspension is added at a ratio of 2% to 3% of the volume of aquaculture wastewater.