Plant root system controllable uniform electromagnetic field platform and electromagnetic field exposure experiment method

Abstract

The invention relates to the technical field of microfluidics, and discloses a plant root system controllable uniform electromagnetic field platform and an electromagnetic field exposure experiment method.The platform comprises a Helmholtz coil assembly used for generating a uniform electromagnetic field in a central area; the micro-fluidic chip culture unit is arranged in the coil center working area, and the chip is provided with a seed inlet, a root system growth channel and a culture solution inlet and outlet; the current regulation and control unit is electrically connected with the coil and outputs controllable direct current; and the control unit is electrically connected with the current regulation and control unit, receives the electromagnetic field parameter instruction and converts the electromagnetic field parameter instruction into a current control signal. The method comprises the steps of seed inoculation and culture, placement of a chip into a coil workbench, parameter setting and current output, electromagnetic field exposure and in-situ observation and recording. Microfluidic visual culture and Helmholtz coil uniform magnetic field are integrated, the magnetic field intensity is digitally and accurately regulated and controlled, and the problems that a traditional device is uneven in field intensity and poor in repeatability are solved.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, specifically to a controllable uniform electromagnetic field platform for plant roots and a method for exposing plant roots to electromagnetic fields. Background Technology

[0002] Plant roots are the core organs for nutrient absorption, signal transduction, and growth and development regulation. The relationship between their physiological characteristics and the external physical environment is a research hotspot in plant biology and agricultural physical regulation. The application of electromagnetic fields to plants has a long history of research and remains an attractive research area for developing strategies to control plant growth. Accurately analyzing the effects of electromagnetic fields on root morphogenesis and physiological metabolism is a crucial foundation for developing novel electrostimulation technologies for plants and optimizing crop cultivation environments.

[0003] Traditional devices have uneven spatial distribution of field strength, resulting in large differences in the field received by different regions of the plant root system, leading to poor experimental repeatability and accuracy. Summary of the Invention

[0004] This invention provides a controllable uniform electromagnetic field platform for plant roots and a method for electromagnetic field exposure experiments to solve the above-mentioned problems.

[0005] In a first aspect, the present invention provides a controllable uniform electromagnetic field platform for plant roots, comprising:

[0006] A magnetic field generating unit, which employs a Helmholtz coil assembly to generate a uniformly distributed electromagnetic field in its central region;

[0007] A microfluidic chip culture unit is located in the central working area of ​​the Helmholtz coil assembly, which is used to accommodate plant roots and ensure that the root growth channels are completely covered by the uniform electromagnetic field.

[0008] A current regulation unit, the output terminal of which is electrically connected to the power input terminal of the Helmholtz coil assembly, is used to output controllable DC current to the Helmholtz coil assembly to adjust the intensity of the electromagnetic field.

[0009] The control unit has its signal output terminal electrically connected to the signal input terminal of the current regulation unit. It is used to receive externally set electromagnetic field parameter commands and convert them into corresponding current control signals to drive the current regulation unit to output a corresponding amount of DC current.

[0010] The platform provided by this invention achieves precise control and repeatability assurance for electromagnetic field exposure experiments on plant roots by precisely arranging microfluidic chip culture units within the uniform magnetic field region of a Helmholtz coil assembly and forming a digital closed-loop control link using a control unit and a current regulation unit. The inherent uniform magnetic field characteristics of the Helmholtz coil assembly ensure that roots at different spatial locations within the chip are subjected to an equal intensity of electromagnetic field, fundamentally solving the experimental deviation problem caused by uneven field strength distribution in traditional experimental devices.

[0011] In one alternative implementation, the Helmholtz coil assembly is a one-dimensional Helmholtz coil comprising two identical, coaxially parallel circular coils.

[0012] In one optional implementation, the microfluidic chip culture unit includes:

[0013] The chip body is provided with at least one seed inlet, a root growth channel communicating with the seed inlet, and a culture medium inlet and outlet;

[0014] The extension path of the root growth channel lies within the projection range of the central working area of ​​the Helmholtz coil assembly.

[0015] In one optional embodiment, the microfluidic chip culture unit further includes a culture flask, the chip body is sealed inside the culture flask, and the culture flask is placed on the central worktable of the Helmholtz coil assembly.

[0016] In one optional implementation, the control unit includes:

[0017] The human-computer interaction module is used to receive electromagnetic field parameters input by the user;

[0018] The microcontroller control core is communicatively connected to the human-machine interaction module and is used to convert the electromagnetic field parameters into current control signals.

[0019] A communication interface is used to realize data transmission between the human-computer interaction module and the microcontroller control core.

[0020] In one optional implementation, the human-computer interaction module is a computer, and the communication interface is a serial port or a USB interface.

[0021] In one alternative implementation, the current regulation unit is a programmable DC power supply or a digital current source.

[0022] In one alternative embodiment, the platform further includes a climate chamber, in which the microfluidic chip culture unit is placed, the climate chamber being used to provide the temperature, humidity, and light environment for plant root growth.

[0023] Secondly, the present invention also provides a method for an experiment on electromagnetic field exposure of plant roots, comprising the following steps:

[0024] Plant seeds are placed into the seed inlet of a microfluidic chip, culture medium is introduced, and the chip is placed in a climate chamber to cultivate until the seeds germinate and the roots extend into the growth channel.

[0025] The microfluidic chip culture unit containing germinating seeds is placed on the central worktable of the Helmholtz coil assembly;

[0026] The target electromagnetic field parameters are input into the control unit, which converts the parameters into a current control signal, driving the current regulation unit to output a corresponding amount of DC current to the Helmholtz coil assembly.

[0027] The Helmholtz coil assembly generates a uniform electromagnetic field in the central region, which acts on the plant roots within the microfluidic chip;

[0028] During the electromagnetic field process, the plant root system is observed and recorded in situ using a microfluidic chip.

[0029] In one optional implementation, in the step of inputting the target electromagnetic field parameters through the control unit, converting the parameters into a current control signal, and driving the current regulation unit to output a corresponding magnitude of DC current to the Helmholtz coil assembly, the electromagnetic field parameters include one or more of magnetic field strength and application duration.

[0030] In the step of observing and recording plant roots in situ using a microfluidic chip during the electromagnetic field process, a control group is set up simultaneously to observe and record the growth status of plant roots under conditions without electromagnetic field, and to compare and analyze the results with the experimental group. Attached Figure Description

[0031] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0032] Figure 1 This is a schematic diagram of the overall structure of a controllable uniform electromagnetic field platform for plant roots according to an embodiment of the present invention.

[0033] Figure 2 This is a structural diagram of a magnetic field generating unit in a plant root controllable uniform electromagnetic field platform according to an embodiment of the present invention;

[0034] Figure 3This is a structural diagram of the chip body in a plant root controllable uniform electromagnetic field platform according to an embodiment of the present invention.

[0035] Explanation of reference numerals in the attached figures:

[0036] 1. Magnetic field generating unit; 11. One-dimensional Helmholtz coil; 12. Central worktable;

[0037] 2. Microfluidic chip culture unit; 21. Chip body; 211. Seed inlet; 212. Culture medium inlet and outlet;

[0038] 3. Current regulation unit;

[0039] 4. Control unit. Detailed Implementation

[0040] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] Plant roots are the core organs for nutrient absorption, signal transduction, and growth and development regulation. The relationship between their physiological characteristics and the external physical environment is a research hotspot in plant biology and agricultural physical regulation. The application of electromagnetic fields to plants has a long history of research and remains an attractive research area for developing strategies to control plant growth. Accurately analyzing the effects of electromagnetic fields on root morphogenesis and physiological metabolism is a crucial foundation for developing novel electrostimulation technologies for plants and optimizing crop cultivation environments.

[0042] Traditional devices have uneven spatial distribution of field strength, resulting in large differences in the field received by different regions of the plant root system, leading to poor experimental repeatability and accuracy.

[0043] The following is combined Figures 1 to 3 The following describes embodiments of the present invention.

[0044] According to an embodiment of the present invention, in a first aspect, a controllable uniform electromagnetic field platform for plant roots is provided, comprising a magnetic field generating unit 1, a microfluidic chip culture unit 2, a current regulating unit 3, and a control unit 4. The magnetic field generating unit 1 employs a Helmholtz coil assembly to generate a uniformly distributed electromagnetic field in its central region; the microfluidic chip culture unit 2 is disposed in the central working area of ​​the Helmholtz coil assembly, used to accommodate plant roots and ensure that the root growth channels are completely covered by the uniform electromagnetic field; the output terminal of the current regulating unit 3 is electrically connected to the power input terminal of the Helmholtz coil assembly, used to output controllable direct current to the Helmholtz coil assembly to adjust the intensity of the electromagnetic field; the signal output terminal of the control unit 4 is electrically connected to the signal input terminal of the current regulating unit 3, used to receive externally set electromagnetic field parameter commands and convert them into corresponding current control signals to drive the current regulating unit 3 to output a corresponding magnitude of direct current.

[0045] The magnetic field generating unit 1 employs a Helmholtz coil assembly. The Helmholtz coil assembly consists of at least one pair of coaxially arranged coils, configured such that when a direct current is applied to the coils, a highly uniformly distributed electromagnetic field is generated in the central region between the two coils. The central region of this Helmholtz coil assembly forms a central worktable 12 for placing experimental samples.

[0046] The microfluidic chip culture unit 2 is positioned on the central stage 12 of the Helmholtz coil assembly. The microfluidic chip culture unit 2 contains at least one microfluidic chip, which has internal channel structures for plant root growth. The entire chip is positioned on the central stage 12 of the Helmholtz coil, ensuring that the root growth channels within the chip are completely within the coverage area of ​​the uniform electromagnetic field generated by the coil.

[0047] The output terminal of the current regulating unit 3 is electrically connected to the power input terminal of the Helmholtz coil assembly. The current regulating unit 3 is used to respond to external control signals and output DC current with a set current value to the Helmholtz coil assembly, thereby continuously adjusting the electromagnetic field strength in the central region of the coil by changing the magnitude of the output current.

[0048] The signal output terminal of the control unit 4 is electrically connected to the signal input terminal of the current regulation unit 3. The control unit 4 has the functions of parameter receiving and control signal conversion: on the one hand, the control unit 4 receives electromagnetic field parameter commands (such as magnetic field strength, application duration, etc.) set by the user through its input interface; on the other hand, the control unit 4 converts the parameter commands into corresponding current control signals and sends the control signals to the current regulation unit 3 in real time, thereby driving the current regulation unit 3 to output a corresponding amount of DC current.

[0049] The platform provided in this embodiment achieves precise control and repeatability assurance for plant root electromagnetic field exposure experiments by precisely arranging the microfluidic chip culture unit 2 within the uniform magnetic field region of the Helmholtz coil assembly and forming a digital closed-loop control link using the control unit 4 and the current control unit 3. The inherent uniform magnetic field characteristics of the Helmholtz coil assembly ensure that roots at different spatial locations within the chip are subjected to an equal intensity of electromagnetic field, fundamentally solving the experimental deviation problem caused by uneven field strength distribution in traditional experimental devices.

[0050] A typical operating mode of the platform is as follows: First, the microfluidic chip with germinated and growing plant roots is placed into the central stage 12 of the Helmholtz coil assembly; then, the operator inputs the desired electromagnetic field parameters through the human-machine interface of the control unit 4; the control unit 4 automatically converts the parameters into current commands and transmits them to the current control unit 3; the current control unit 3 then outputs a matching DC power to supply the Helmholtz coil assembly, and a stable and uniform electromagnetic field is generated in the central region of the coil and acts on the roots within the chip; during the application of the electromagnetic field, external imaging equipment such as stereomicroscopes and confocal microscopes can be used to observe the phenotypes of plant roots, such as elongation, branching, and geotropism, in situ and in real time through the transparent structure of the microfluidic chip.

[0051] In one embodiment, the Helmholtz coil assembly is a one-dimensional Helmholtz coil 11 comprising two identical, coaxially parallel circular coils.

[0052] The first and second circular coils have the same geometric parameters, including the same coil radius, the same number of turns, the same wire diameter, and the same resistance value. The two circular coils are arranged coaxially, with their axes coinciding. The planes containing the two circular coils are parallel to each other, and the axial distance between the two planes is equal to the coil radius.

[0053] The two circular coils are wound in the same direction to ensure that when the current control unit 3 supplies power to the two coils, the current flows in the same direction in the two coils, so that the magnetic fields generated by the two coils are superimposed and enhanced in the axial direction.

[0054] In one embodiment, the microfluidic chip culture unit 2 includes a chip body 21, on which at least one seed inlet 211, a root growth channel communicating with the seed inlet 211, and a culture medium inlet and outlet 212 are provided; the extension path of the root growth channel is located within the projection range of the central working area of ​​the Helmholtz coil assembly.

[0055] In this embodiment, the microfluidic chip culture unit 2 includes a chip body 21. The chip body 21 is made of a light-transmitting polymer material, such as polydimethylsiloxane, polymethyl methacrylate, or cyclic olefin copolymer, to ensure good visibility inside the chip and facilitate in-situ optical observation of plant roots during experiments.

[0056] The chip body 21 has at least one seed inlet 211, and a root growth channel is also provided inside the chip body 21. The root growth channel is a microchannel, one end of which is connected to the bottom of the seed inlet 211. The root growth channel is constructed to provide space for the extension and growth of the taproot and lateral roots after the plant seed germinates. The root growth channel can be arranged in a straight line, serpentine, or radial pattern to meet the requirements of different experimental purposes for root space arrangement. The chip body 21 also has a culture medium inlet and outlet 212, which are in fluid communication with the root growth channel and are used to introduce sterile culture medium, nutrient solution, or buffer solution into the chip, and to discharge waste liquid. Multiple culture medium inlets and outlets 212 can be set and distributed at different positions in the root growth channel to form a continuous perfusion culture mode, ensuring nutrient supply and metabolic waste removal in the root growth area.

[0057] The projection of the root growth channel's extension path onto the horizontal plane lies entirely within the projection range of the central working area of ​​the Helmholtz coil assembly. This embodiment ensures that the entire area of ​​the root growth channel is covered by the uniform electromagnetic field generated by the Helmholtz coil by precisely aligning and fixing the chip body 21 on the central worktable 12.

[0058] In one embodiment, the microfluidic chip culture unit 2 further includes a culture flask, with the chip body 21 sealed inside the culture flask and the culture flask placed on the central stage 12 of the Helmholtz coil assembly.

[0059] The culture flask is a transparent container made of optical-grade glass or transparent polymer materials, such as borosilicate glass, polystyrene, or polycarbonate. The flask has sufficient transparency to meet the needs of optical observation of the chip and plant roots inside during experiments.

[0060] This embodiment achieves physical isolation between the microfluidic chip body 21 and the external environment by using a culture flask as an external container. The culture flask provides a microenvironment for the chip body 21, avoiding the problems of evaporation, contamination, and temperature and humidity fluctuations faced by open-cell chips during long-term experiments.

[0061] In one embodiment, the control unit 4 includes a human-machine interaction module, a microcontroller control core, and a communication interface. The human-machine interaction module is used to receive electromagnetic field parameters input by the user. The microcontroller control core is communicatively connected to the human-machine interaction module and is used to convert the electromagnetic field parameters into current control signals. The communication interface is used to realize data transmission between the human-machine interaction module and the microcontroller control core.

[0062] The human-computer interaction module receives electromagnetic field parameters input by the user. This module has a parameter input interface, which can be displayed on a physical panel or a virtual graphical interface. Through this interface, the user sets the desired electromagnetic field conditions to be applied to the plant roots, including but not limited to magnetic field strength, application duration, application mode (e.g., continuous application, intermittent application, stepped application), experiment number, and remarks. The human-computer interaction module converts the user-input parameters into a transmittable digital command format.

[0063] The microcontroller control core and the human-machine interface module communicate via a communication interface. The microcontroller control core has a built-in microprocessor and memory unit, which contains a pre-defined algorithm for converting electromagnetic field parameters to current control signals. The microcontroller control core receives electromagnetic field parameter commands from the human-machine interface module, parses the commands according to a preset conversion relationship, and maps them into corresponding current control signals. These current control signals are either analog voltage signals or digital pulse signals, and their amplitude, frequency, or duty cycle have a deterministic functional relationship with the target current value.

[0064] The communication interface is used to realize data transmission between the human-computer interaction module and the microcontroller control core.

[0065] In one embodiment, the human-computer interaction module is a computer, and the communication interface is a serial port or a USB interface.

[0066] In one embodiment, the current regulation unit 3 is a programmable DC power supply or a digital current source.

[0067] In this embodiment, the current regulation unit 3 uses a programmable DC power supply or a digital current source. The current regulation unit 3 has a digital control interface, which can receive analog or digital control signals from the control unit 4 and precisely adjust the DC current value of its output port according to the control signals.

[0068] In one embodiment, the platform further includes a climate chamber, in which the microfluidic chip culture unit 2 is placed, and the climate chamber is used to provide the temperature, humidity and light environment for plant root growth.

[0069] For example, the climate chamber may contain a refrigeration unit and a heating unit, such as a compressor refrigeration system and a PTC ceramic heater, working in conjunction with an internal circulating fan and temperature sensors (such as platinum resistance thermometers or thermocouples) to form a closed-loop temperature control system. Alternatively, the climate chamber may contain a humidification unit and a dehumidification unit. The humidification unit can use an ultrasonic atomizing humidifier or an electrode-type steam humidifier, while the dehumidification unit can use condensation dehumidification or rotary dehumidification. An illumination module may be installed on the top wall or below the shelves inside the climate chamber. The illumination module uses a full-spectrum LED light source, including multiple light quality chips such as red, blue, far-red, and white light, and the quantum flux density of each color can be independently adjusted.

[0070] Secondly, this embodiment also provides a method for an electromagnetic field exposure experiment on plant roots, comprising the following steps:

[0071] Plant seeds are placed into the seed inlet 211 of the microfluidic chip, culture medium is introduced, and the chip is placed in a climate chamber to cultivate until the seeds germinate and the roots extend into the growth channel.

[0072] Take the plant seeds to be tested, selected from model plants or crops such as Arabidopsis thaliana, rice, tobacco, maize, or wheat. Place the microfluidic chip in a sterile operating table and use a micropipette or a special inoculation needle to insert a single seed through the seed inlet 211 of the chip body 21. Introduce sterile culture medium into the chip through the culture medium inlet and outlet 212 on the chip body 21. The culture medium is selected according to the plant species and experimental purpose. Place the seed-inoculated and culture-injected microfluidic chip into a culture flask and seal the flask. Transfer the sealed culture flask to a climate chamber. The climate chamber is preset with environmental conditions suitable for plant seed germination and seedling growth. Under these conditions, the seed is incubated statically, and the germination status is observed daily. Continue incubation until the seed germinates, the radicle emerges, and extends along the root growth channel.

[0073] The microfluidic chip culture unit 2 containing germinating seeds is placed on the central stage 12 of the Helmholtz coil assembly;

[0074] The obtained microfluidic chip culture unit 2 containing germinated seeds was removed from the climate chamber and transferred as a whole to the central stage 12 of the Helmholtz coil assembly. The central stage 12 is located in the central region between the two circular coils, which is the spatial position with optimal magnetic field uniformity.

[0075] The operator places the culture flask stably on the support surface of the central worktable 12 and adjusts the position of the culture flask so that its central axis roughly coincides with the axis of the Helmholtz coil. After placement, the operator checks the orientation of the microfluidic chip body 21 inside the culture flask to ensure that the bottom surface of the chip is horizontal and the extension direction of the root growth channel is compatible with the direction of the observation optical path.

[0076] The target electromagnetic field parameters are input through the control unit 4, which converts the parameters into a current control signal to drive the current regulation unit 3 to output a corresponding amount of DC current to the Helmholtz coil assembly.

[0077] The Helmholtz coil assembly generates a uniform electromagnetic field in the central region, which acts on the plant roots within the microfluidic chip;

[0078] During the electromagnetic field process, the plant root system is observed and recorded in situ using a microfluidic chip.

[0079] The method described in this embodiment organically integrates microfluidic chip visualization cultivation technology with Helmholtz coil uniform magnetic field generation technology to form a standardized experimental procedure for plant electromagnetic field exposure. This method fully leverages the synergistic advantages of microfluidic chips (allowing for in-situ observation), Helmholtz coils (providing uniform magnetic fields), and digitally programmable control, enabling researchers to accurately analyze the regulatory effects of electromagnetic fields on plant root growth and development under well-defined, controllable, and repeatable physical field boundary conditions.

[0080] In one embodiment, in the step of inputting target electromagnetic field parameters through control unit 4, converting the parameters into current control signals, and driving current regulation unit 3 to output a corresponding amount of DC current to Helmholtz coil assembly, the electromagnetic field parameters include one or more of magnetic field strength and application duration.

[0081] During the process of electromagnetic field application, in the step of in-situ observation and recording of plant roots using a microfluidic chip, a control group was simultaneously set up to observe and record the growth status of plant roots under conditions without electromagnetic field, and to compare and analyze the results with the experimental group.

[0082] Although embodiments of the invention have been described in conjunction with the accompanying drawings, those skilled in the art can make various modifications and variations without departing from the spirit and scope of the invention, and such modifications and variations all fall within the scope defined by the appended claims.

Claims

1. A controllable uniform electromagnetic field platform for plant roots, characterized in that, include: A magnetic field generating unit (1) is provided, which employs a Helmholtz coil assembly to generate a uniformly distributed electromagnetic field in its central region. Microfluidic chip culture unit (2), the microfluidic chip culture unit (2) is set in the central working area of ​​the Helmholtz coil assembly, and is used to accommodate plant roots and make the root growth channel completely covered by the uniform electromagnetic field; The current regulation unit (3) is electrically connected to the power input terminal of the Helmholtz coil assembly and is used to output controllable DC power to the Helmholtz coil assembly to adjust the intensity of the electromagnetic field. The control unit (4) is electrically connected to the signal input terminal of the current regulation unit (3) to receive externally set electromagnetic field parameter instructions and convert them into corresponding current control signals to drive the current regulation unit (3) to output a corresponding amount of DC current.

2. The plant root controllable uniform electromagnetic field platform according to claim 1, characterized in that, The Helmholtz coil assembly is a one-dimensional Helmholtz coil (11), comprising two identical circular coils arranged coaxially and parallel to each other.

3. The plant root controllable uniform electromagnetic field platform according to claim 1 or 2, characterized in that, The microfluidic chip culture unit (2) includes: The chip body (21) is provided with at least one seed inlet (211), a root growth channel connected to the seed inlet (211), and a culture medium inlet and outlet (212). The extension path of the root growth channel lies within the projection range of the central working area of ​​the Helmholtz coil assembly.

4. The plant root controllable uniform electromagnetic field platform according to claim 3, characterized in that, The microfluidic chip culture unit (2) also includes a culture bottle, the chip body (21) is sealed inside the culture bottle, and the culture bottle is placed on the central worktable (12) of the Helmholtz coil assembly.

5. The controllable uniform electromagnetic field platform for plant roots according to claim 1, characterized in that, The control unit (4) includes: The human-computer interaction module is used to receive electromagnetic field parameters input by the user; The microcontroller control core is communicatively connected to the human-machine interaction module and is used to convert the electromagnetic field parameters into current control signals. A communication interface is used to realize data transmission between the human-computer interaction module and the microcontroller control core.

6. The plant root controllable uniform electromagnetic field platform according to claim 5, characterized in that, The human-computer interaction module is a computer, and the communication interface is a serial port or a USB interface.

7. The plant root controllable uniform electromagnetic field platform according to claim 1, characterized in that, The current regulation unit (3) is a programmable DC power supply or a digital current source.

8. The plant root controllable uniform electromagnetic field platform according to claim 1, characterized in that, The platform also includes a climate chamber, in which the microfluidic chip culture unit (2) is placed. The climate chamber is used to provide the temperature, humidity and light environment for plant root growth.

9. A method for an electromagnetic field exposure experiment on plant roots, characterized in that, Includes the following steps: Plant seeds are placed into the seed inlet (211) of the microfluidic chip, and culture medium is introduced. The chip is placed in a climate chamber and cultured until the seeds germinate and the roots extend into the growth channel. The microfluidic chip culture unit (2) containing germinating seeds is placed on the central worktable (12) of the Helmholtz coil assembly; The target electromagnetic field parameters are input through the control unit (4), and the control unit (4) converts the parameters into a current control signal, driving the current regulation unit (3) to output a corresponding amount of DC current to the Helmholtz coil assembly; The Helmholtz coil assembly generates a uniform electromagnetic field in the central region, which acts on the plant roots within the microfluidic chip; During the electromagnetic field process, the plant root system is observed and recorded in situ using a microfluidic chip.

10. The method for plant root electromagnetic field exposure experiment according to claim 9, characterized in that: In the step of inputting the target electromagnetic field parameters through the control unit (4), converting the parameters into a current control signal, and driving the current regulation unit (3) to output a corresponding amount of DC current to the Helmholtz coil assembly, the electromagnetic field parameters include one or more of magnetic field strength and application duration; In the step of observing and recording plant roots in situ using a microfluidic chip during the electromagnetic field process, a control group is set up simultaneously to observe and record the growth status of plant roots under conditions without electromagnetic field, and to compare and analyze the results with the experimental group.

Images