Soil conditioner for continuous cropping of sugar beets, its preparation method and application
By preparing a complex microbial strain containing modified plant-derived extracts, modified carriers, and modified enhancers, a dual defense line was formed, solving the problem of soil quality degradation caused by continuous sugar beet cropping, and achieving improvements in soil health and the yield and quality of sugar beets.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
- Filing Date
- 2026-01-23
- Publication Date
- 2026-05-26
AI Technical Summary
The current continuous cropping of sugar beets has led to an imbalance in the soil microbial community, frequent occurrence of soil-borne diseases, and deterioration of soil physical and chemical properties. Existing regulators have limitations such as single bacterial species, difficulty in maintaining activity, and poor adaptability, making it difficult to form a large-scale management plan. Moreover, chemical agents are prone to increasing the drug resistance of pathogens and degrading the soil ecological function.
A soil conditioner for continuously cropped sugar beets was developed. By preparing modified plant-derived extracts, modified carriers, and modified enhancers, combined with compound microbial strains, a dual defense line of "plant-derived antibacterial + microbial antagonism" was formed to synergistically improve soil health.
It significantly inhibits soil-borne diseases, improves soil structure, enhances microbial activity, increases sugar beet yield and quality, and builds a sustainable industrial model of green planting, high-efficiency sugar production, and resource recycling.
Smart Images
Figure CN122080943A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of soil conditioner technology, specifically to a soil conditioner for continuously cropped sugar beets, its preparation method, and its application. Background Technology
[0002] Sugar beets are an important sugar crop in northern my country, with an annual planting area of approximately 4 million mu (667,000 hectares), mainly concentrated in Inner Mongolia and Xinjiang. Continuous cropping directly leads to problems such as soil microbial imbalance, frequent soil-borne diseases, and deterioration of soil physical and chemical properties. Specifically, this manifests as a 7.4% to 28.2% reduction in sugar beet yield, a 0.5 to 2.0 degree sugar content decrease, and diseases such as root rot and brown spot become more severe with increasing years of continuous cropping, seriously restricting the sustainable development of the sugar beet industry.
[0003] Currently, the control of continuous cropping obstacles and diseases in sugar beets mainly relies on biological agents and chemical agents, but these methods have significant limitations in practical application. While traditional chemical agents can suppress diseases in the short term, they easily lead to increased drug resistance in pathogens and degradation of soil ecological functions, and are not in line with the development direction of green agriculture. Existing biological agents on the market generally suffer from problems such as single strains, difficulty in maintaining activity, poor adaptability, and limited functions. In addition, existing regulators often ignore the synergistic effect between plant-derived antibacterial components, carriers, and nutrient components, resulting in unstable practical application effects and making it difficult to form large-scale control solutions.
[0004] Therefore, developing a multifunctional composite soil conditioner that integrates antibacterial, growth-promoting, soil structure-improving, and microbial activity-enhancing properties has become an urgent task. Such a conditioner needs to combine plant-derived antibacterial substances, functional microorganisms, and organic amendments to target and address soil quality degradation caused by continuous cropping through multi-component synergistic effects. Ultimately, this will reduce reliance on chemical pesticides, restore soil ecology, and improve sugar beet yield and quality, providing key technological support for building a sustainable industrial model of "green planting - high-efficiency sugar production - resource recycling" in sugar beet producing areas. Summary of the Invention
[0005] In view of the shortcomings of the prior art, the purpose of this invention is to provide a soil conditioner for continuous cropping of sugar beets, its preparation method and application.
[0006] To achieve the above objectives, the present invention provides the following technical solution: The preparation method of soil conditioner for continuous cropping of sugar beets includes the following preparation steps: S1. Inoculate the compound bacterial strains separately into liquid culture medium and culture them with shaking at 28-30℃ and 180-200rpm for 45-48h. After mixing, obtain a live bacterial solution. S2. By weight, add 5-8 parts of modified plant extract and 45-50 parts of modified carrier to 40-50 parts of modified reinforcing agent, and stir at 200-300 r / min for 15-20 min to obtain modified mixture; S3. Add the live bacteria solution obtained in step S1 to the modified mixture while stirring at a speed of 200-300 r / min, control the moisture content at 25-30%, then granulate using a disc granulator, and air dry at 38-40℃ for 6-8 hours to obtain the continuous cropping sugar beet soil conditioner. The preparation of modified plant-derived extracts includes the following steps: S11. By weight, mix 8-10 parts of Coptis chinensis powder and 8-10 parts of Sophora flavescens powder, add 180-200 parts of ethanol solution, and extract by stirring and reflux at 180-200 rpm for 1-2 hours under a water bath at 58-60℃. S12. Filter the solution obtained in step S11 using a Buchner funnel, add 130-150 parts of ethanol solution back to the filter residue, repeat the reflux extraction for 1-2 hours, and combine the filtrates obtained in step S11 and step S12. S13. Under conditions of 60℃ and -0.03MPa, the combined filtrates are concentrated to one-tenth of their original volume to obtain a thick extract; S14. Add 0.8-1 parts citric acid, 1.2-1.5 parts copper sulfate and 0.3-0.5 parts Tween-80 to the thick extract, and stir at 48-50℃ for 25-30 minutes to obtain the modified plant-derived extract.
[0007] Preferably, the preparation of the modified support includes the following steps: S21. By weight, mix 20-25 parts of diatomaceous earth and 25-28 parts of biochar, calcine in a muffle furnace at 450-500℃ for 1-2 hours, cool and pass through a 100-mesh sieve to obtain the basic carrier. S22. Dissolve 0.8-1 part of glacial acetic acid in 35-40 parts of deionized water, slowly add 1.5-2 parts of chitosan powder, stir at 250-300 rpm for 1-2 hours in a 40℃ water bath until completely dissolved, then add 0.8-1 part of calcium nitrate and continue stirring until dissolved; S23. Add the base carrier obtained in step S21 to the mixture obtained in step S22, stir at 450-500 rpm, let it fully soak, let it stand at room temperature for 1-2 hours, dry it at 100-105℃ for 3-4 hours, and pulverize it through an 80-mesh sieve to obtain the modified carrier.
[0008] Preferably, the preparation of the modified reinforcing agent includes the following steps: S31. By weight, mix 3-6 parts of polyglutamic acid with 5-8 parts of anhydrous ethanol and stir until dispersed to obtain a premix; S32. Add 15-18 parts of humic acid and 10-15 parts of sodium alginate to the premix, and stir at 30-50 rpm for 10-15 min to obtain the preliminary modifier; S33. Add 8-12 parts of potassium dihydrogen phosphate, 2-5 parts of zinc sulfate, and 4-7 parts of potassium humate to the preliminary modifier obtained in step S32 and stir at 30-50 rpm for 20-30 minutes to obtain the modified reinforcing agent.
[0009] Preferably, the compound microbial strain consists of 5-7 parts of Bacillus subtilis, 3-5 parts of Trichoderma viride, 2-4 parts of Arthrobacter spheroidae, and 2-3 parts of Bacillus brevis.
[0010] Preferably, the particle size of the granulators produced by the disc granulator in step S3 is controlled to be 2-4 mm.
[0011] Preferably, the ethanol solution in steps S11 and S12 has a mass concentration of 70%.
[0012] Preferably, the stirring speed in step S14 is 250-300 rpm.
[0013] Soil conditioner for continuously cropped sugar beets is prepared by the above-mentioned preparation method.
[0014] Application of soil conditioner for continuous cropping of sugar beets in sugar beet cultivation.
[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. The modified plant-derived extract of this invention can effectively inhibit bacteria and has strong antibacterial stability in soil. It works synergistically with live bacteria liquid to form a dual defense line of "plant-derived antibacterial + microbial antagonism", effectively inhibiting soil-borne pathogens and reducing the incidence of diseases.
[0016] 2. The modified carrier of this invention possesses excellent biocompatibility and ion exchange capacity, effectively adsorbing and slowly releasing live bacteria and plant-derived components, prolonging the action time, while simultaneously improving soil aggregate structure and enhancing water and fertilizer retention capacity. The modified carrier and the modifier synergistically enhance the stability of the microbial agent particles in the soil, providing the necessary nutrients for the growth of functional microorganisms, promoting their rapid colonization and reproduction, and simultaneously activating beneficial native soil microorganisms to build a healthy micro-ecology.
[0017] 3. The modified plant-derived extract, modified carrier, and modified enhancer of this invention work synergistically to form a three-in-one regulatory system of "antibacterial-growth-improvement", which significantly improves the health level of soil and crop yield in continuous sugar beet cropping. Attached Figure Description
[0018] Figure 1 This is a process flow diagram of the preparation process of the continuous cropping sugar beet soil conditioner of the present invention; Figure 2 This is a flow chart illustrating the preparation process of the modified plant-derived extract of the present invention. Figure 3 This is a flow chart of the preparation process of the modified carrier of the present invention; Figure 4 This is a flowchart illustrating the preparation process of the modified reinforcing agent of this invention. Detailed Implementation
[0019] The present invention will now be clearly and completely described in conjunction with embodiments thereof. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.
[0020] Please see Figure 1-4 The present invention provides a technical solution: Example 1 Preparation method of soil conditioner for continuous cropping of sugar beets: Before preparing the soil conditioner for continuously cropped sugar beets, the following steps are taken: Preparation of modified plant-derived extracts, modified carriers, and modified enhancers are performed: The preparation of modified plant-derived extracts includes the following steps: S11. Mix 8-g Coptis chinensis powder and 8-g Sophora flavescens powder, add 180g of 70% ethanol solution, and extract by stirring and reflux at 180rpm for 1h under a water bath at 58℃. S12. Filter the solution obtained in step S11 using a Buchner funnel, add 130g of 70% ethanol solution back to the filter residue, repeat the reflux extraction for 1h, and combine the filtrates obtained in steps S11 and S12. S13. Under conditions of 60℃ and -0.03MPa, the combined filtrates are concentrated to one-tenth of their original volume to obtain a thick extract; S14. Add 0.8g citric acid, 1.2g copper sulfate and 0.3g Tween-80 to the thick extract, and stir for 25 minutes at 48℃ and 250rpm to obtain the modified plant-derived extract. The preparation of the modified carrier includes the following steps: S21. Mix 20g of diatomaceous earth and 25g of biochar, calcine in a muffle furnace at 450℃ for 1h, cool and pass through a 100-mesh sieve to obtain the basic carrier; S22. Dissolve 0.8g of glacial acetic acid in 35g of deionized water, slowly add 1.5g of chitosan powder, stir at 250rpm for 1h in a 40℃ water bath until completely dissolved, then add 0.8g of calcium nitrate and continue stirring until dissolved; S23. Add the base carrier obtained in step S21 to the mixture obtained in step S22, stir at 450 rpm, let it fully soak, let it stand at room temperature for 1 hour, dry it at 100°C for 3 hours, and pulverize it through an 80-mesh sieve to obtain the modified carrier. The preparation of the modified reinforcing agent includes the following steps: S31. Mix 3g of polyglutamic acid with 5g of anhydrous ethanol and stir until dispersed to obtain a premix. S32. Add 15g of humic acid and 10g of sodium alginate to the premix, and stir at 30rpm for 10min to obtain the preliminary modifier; S33. Add 8g of potassium dihydrogen phosphate, 2g of zinc sulfate, and 4g of potassium humate to the preliminary modifier obtained in step S32 and stir at 30 rpm for 20 min to obtain the modified reinforcing agent; S1. Preparation of live bacterial solution: The compound bacterial strain (5g Bacillus subtilis, 3g Trichoderma viride, 2g Arthrobacter spheroidae, 2g Bacillus brevis) was inoculated into liquid culture medium and cultured with shaking at 28℃ and 180rpm for 45h. After mixing, the live bacterial solution was obtained. S2. Preparation of modified mixture: Add 5g of modified plant extract and 45g of modified carrier to 40g of modified reinforcing agent, and stir at 200r / min for 15min to obtain modified mixture; S3. Preparation of the regulator: Add the live bacteria solution obtained in step S1 to the modified mixture while stirring at a speed of 200 r / min. Control the moisture content at 25%, then granulate using a disc granulator to control the particle size at 2 mm. Air dry at 38℃ for 6 h to obtain the soil regulator for continuous cropping sugar beets.
[0021] Example 2 Preparation method of soil conditioner for continuous cropping of sugar beets: Before preparing the soil conditioner for continuously cropped sugar beets, the following steps are taken: Preparation of modified plant-derived extracts, modified carriers, and modified enhancers are performed: The preparation of modified plant-derived extracts includes the following steps: S11. Mix 10g of Coptis chinensis powder and 10g of Sophora flavescens powder, add 200g of 70% ethanol solution, and extract by stirring and reflux at 200rpm for 2h under a water bath at 60℃. S12. Filter the solution obtained in step S11 using a Buchner funnel, add 150g of 70% ethanol solution back to the filter residue, repeat the reflux extraction for 2h, and combine the filtrates obtained in steps S11 and S12. S13. Under conditions of 60℃ and -0.03MPa, the combined filtrates are concentrated to one-tenth of their original volume to obtain a thick extract; S14. Add 1g citric acid, 1.5g copper sulfate and 0.5g Tween-80 to the thick extract, and stir for 30min at 50℃ and 300rpm to obtain the modified plant-derived extract. The preparation of the modified carrier includes the following steps: S21. Mix 25g of diatomaceous earth and 28g of biochar, calcine in a muffle furnace at 500℃ for 2h, cool and pass through a 100-mesh sieve to obtain the basic carrier; S22. Dissolve 1g of glacial acetic acid in 40g of deionized water, slowly add 2g of chitosan powder, stir at 300rpm for 2 hours in a 40℃ water bath until completely dissolved, then add 1g of calcium nitrate and continue stirring until dissolved. S23. Add the base carrier obtained in step S21 to the mixture obtained in step S22, stir at 500 rpm, let it fully soak, let it stand and age at room temperature for 2 hours, dry it at 105℃ for 4 hours, and pulverize it through an 80-mesh sieve to obtain the modified carrier. The preparation of the modified reinforcing agent includes the following steps: S31. Mix 6g of polyglutamic acid with 8g of anhydrous ethanol and stir until dispersed to obtain a premix; S32. Add 18g of humic acid and 15g of sodium alginate to the premix, and stir at 50rpm for 15min to obtain the preliminary modifier; S33. Add 12g of potassium dihydrogen phosphate, 5g of zinc sulfate, and 7g of potassium humate to the preliminary modifier obtained in step S32 and stir at 50 rpm for 30 min to obtain the modified reinforcing agent. S1. Preparation of live bacterial solution: The compound bacterial strain (7g Bacillus subtilis, 5g Trichoderma viride, 4g Arthrobacter spheroidae, and 3g Bacillus brevis) was inoculated into liquid culture medium and cultured with shaking at 30℃ and 200rpm for 48h. After mixing, the live bacterial solution was obtained. S2. Preparation of modified mixture: Add 8g of modified plant extract and 50g of modified carrier to 50g of modified reinforcing agent, and stir at 300r / min for 20min to obtain modified mixture; S3. Preparation of the regulator: Add the live bacteria solution obtained in step S1 to the modified mixture while stirring at a speed of 300 r / min. Control the moisture content at 30%. Then granulate the mixture using a disc granulator to control the particle size at 4 mm. Air dry at 40℃ for 8 hours to obtain the soil regulator for continuous cropping sugar beets.
[0022] Example 3 Preparation method of soil conditioner for continuous cropping of sugar beets: Before preparing the soil conditioner for continuously cropped sugar beets, the following steps are taken: Preparation of modified plant-derived extracts, modified carriers, and modified enhancers are performed: The preparation of modified plant-derived extracts includes the following steps: S11. Mix 8.5g of Coptis chinensis powder and 8.5g of Sophora flavescens powder, add 185g of 70% ethanol solution, and extract by stirring and reflux at 185rpm for 1.5h in a 59℃ water bath. S12. Filter the solution obtained in step S11 using a Buchner funnel, add 135g of 70% ethanol solution back to the filter residue, and repeat the reflux extraction for 1.5h. Combine the filtrates obtained in steps S11 and S12. S13. Under conditions of 60℃ and -0.03MPa, the combined filtrates are concentrated to one-tenth of their original volume to obtain a thick extract; S14. Add 0.85g citric acid, 1.3g copper sulfate and 0.35g Tween-80 to the thick extract, and stir for 26min at 49℃ and 260rpm to obtain the modified plant-derived extract. The preparation of the modified carrier includes the following steps: S21. Mix 21g of diatomaceous earth and 26g of biochar, calcine in a muffle furnace at 460℃ for 1.5h, cool and pass through a 100-mesh sieve to obtain the basic carrier; S22. Dissolve 0.85g of glacial acetic acid in 36g of deionized water, slowly add 1.6g of chitosan powder, stir at 260rpm for 1.5h in a 40℃ water bath until completely dissolved, then add 0.85g of calcium nitrate and continue stirring until dissolved; S23. Add the base carrier obtained in step S21 to the mixture obtained in step S22, stir at 460 rpm, let it fully soak, let it stand and age at room temperature for 1.5 h, dry at 101 °C for 3.5 h, and pulverize it through an 80 mesh sieve to obtain the modified carrier. The preparation of the modified reinforcing agent includes the following steps: S31. Mix 4g of polyglutamic acid with 6g of anhydrous ethanol and stir until dispersed to obtain a premix. S32. Add 16g of humic acid and 11g of sodium alginate to the premix, and stir at 35rpm for 11min to obtain the preliminary modifier; S33. Add 9g of potassium dihydrogen phosphate, 3g of zinc sulfate, and 5g of potassium humate to the preliminary modifier obtained in step S32 and stir at 35 rpm for 21 min to obtain the modified reinforcing agent; S1. Preparation of live bacterial solution: The compound bacterial strain (5.5g Bacillus subtilis, 3.5g Trichoderma viride, 2.5g Arthrobacter spheroidae, and 2.2g Bacillus brevis) was inoculated into liquid culture medium and cultured with shaking at 29℃ and 185rpm for 46h. After mixing, the live bacterial solution was obtained. S2. Preparation of modified mixture: Add 6g of modified plant extract and 46g of modified carrier to 42g of modified reinforcing agent, and stir at 220r / min for 16min to obtain modified mixture; S3. Preparation of the regulator: Add the live bacteria solution obtained in step S1 to the modified mixture while stirring at a speed of 220 r / min. Control the moisture content at 26%. Then granulate using a disc granulator to control the particle size at 3 mm. Air dry at 39℃ for 6.5 h to obtain the soil regulator for continuous cropping sugar beets.
[0023] The bacterial strains used in Examples 1-3 above were all purchased from Zhongnong Lvkang (Beijing) Biotechnology Co., Ltd. The product models for *Trichoderma viride*, *Bacillus brevis*, *Bacillus subtilis*, and *Arthrobacter spheroidae* were bio-68043, bio-19298, bio-00028, and bio-19619, respectively.
[0024] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that no modified plant extract was added in this comparative example; the other steps are exactly the same in Comparative Example 1 and Example 1.
[0025] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that no modified carrier or modified reinforcing agent was added in this comparative example; the other steps are exactly the same in Comparative Example 2 and Example 1.
[0026] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that no modifier was added in this comparative example; the other steps are exactly the same in Comparative Example 3 and Example 1.
[0027] Performance testing: The inhibitory effect of the soil regulator on common soil-borne pathogens in continuously cropped sugar beet soil was evaluated using the plate inhibition zone method. 1g of the continuously cropped sugar beet soil regulator obtained in Examples 1-3 and Comparative Examples 1-3 was added to 10mL of sterile water, shaken for 30min, and centrifuged to obtain the supernatant as the extract (concentration 0.1g / mL). Fusarium and Rhizoctonia solani were inoculated into the center of PDA plates, and 6mm diameter filter paper discs were placed around the plates. 100μL of the extract was added to each plate, and the plates were incubated at 28℃ for 5 days. The diameter of the inhibition zone was measured. The results are shown in Table 1 below. Table 1 The average diameter of the inhibition zone of the continuously cropped sugar beet soil regulators obtained in Examples 1-3 was between 14.8-15.2 mm, with minimal variation (range 0.4 mm), indicating a stable preparation process and consistent antibacterial effect. The inhibition zone diameter of the comparative examples was significantly lower; the average diameter of the inhibition zone in the example group was approximately 92% higher than that in the comparative group. The inhibition zone diameter of the continuously cropped sugar beet soil regulators obtained in Examples 1-3 against the two pathogens was significantly larger than that in Comparative Examples 1-3, indicating that the synergistic effect of the modified plant-derived extract, modified carrier, and modified enhancer effectively enhanced the antibacterial ability, verifying the effectiveness of the dual defense line of "plant-derived antibacterial + microbial antagonism." The modified carrier and modified enhancer synergistically improved the stability of the inoculant particles in the soil, providing the nutrients needed for the growth of functional microorganisms, promoting their rapid colonization and reproduction, and simultaneously activating beneficial native soil microorganisms.
[0028] Soil sample analysis was used to evaluate the effects of soil regulators on soil physicochemical properties and microbial communities. Soil samples from the 0-20 cm layer were collected at the sugar beet harvest period, mixed, and various indicators were measured. Soil aggregate stability was determined using the wet sieving method to measure the proportion of water-stable aggregates >0.25 mm; field water holding capacity was determined using the ring sieving method; organic matter content was determined using the potassium dichromate oxidation method; and microbial community structure was analyzed using high-throughput sequencing (16S rRNA and ITS) to analyze bacterial and fungal diversity. Each treatment was repeated three times, and the average value was taken. The data are shown in Table 2 below. Table 2 The data in Table 2 show that the average values for aggregate stability, field water holding capacity, organic matter, bacterial index, and fungal index in Examples 1-3 were 46.0, 27.5, 25.9, 6.7, and 4.5, respectively, while the average values for the comparative examples were 34.6, 23.0, 21.5, 5.4, and 3.3, respectively. The soil aggregate stability, field water holding capacity, organic matter content, and microbial biomass carbon in Examples 1-3 were significantly higher than those in the comparative examples, indicating that the modified plant-derived extract, modified carrier, and modified enhancer synergistically improved soil structure and fertility, prolonged the effective duration of the active ingredients, provided nutrition, and synergistically promoted microbial reproduction and soil health. Microbial diversity analysis showed that the Shannon indices for bacteria and fungi were higher in the examples, indicating a richer and more balanced microbial community, an increased proportion of beneficial microorganisms (such as actinomycetes and Trichoderma viride), and a decreased proportion of pathogenic bacteria (such as Fusarium).
[0029] A sugar beet cultivation experiment was conducted to measure growth indicators, yield, and quality. Growth regulators were applied before sowing. Plant height, leaf area, and chlorophyll content (SPAD value) were measured during the mid-growth stage (75 days). Tuber yield and sugar content were measured at harvest (150 days) using a refractometer. The results are shown in Table 3 below. Table 3 Note: Uppercase letters in the table indicate significant differences at the 0.01 level, and lowercase letters indicate significant differences at the 0.05 level.
[0030] The average values of plant height, leaf area index, chlorophyll content, dry matter accumulation, tuber yield, and sugar content of the beet in the example were 75.77, 5.38, 52.20, 12459.05, 50508.58, and 16.07, respectively. The average values of the comparative examples were 69.37, 5.95, 44.83, 11055.97, 47293.75, and 15.27, respectively. The data in Table 3 were compared between the examples (n=3) and the comparative examples (n=3), and a one-way ANOVA analysis was performed using SPASS 19.0. Analysis showed that beet plant height, chlorophyll SPAD value, dry matter accumulation, tuber yield, and sugar content were highly significant between the examples and the comparative examples (p<0.01), and leaf area index was significant (p<0.05). This indicates that the differences between the examples and the comparative examples were statistically significant, further verifying the significant effect of the soil regulator of the present invention in promoting beet growth, increasing yield, and improving quality. It also shows that the synergistic effect of the modified plant-derived extract, modified carrier, and modified enhancer effectively promotes beet growth and sugar accumulation, and the synergistic system is crucial for promoting growth.
[0031] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A method for preparing a soil conditioner for continuously cropped sugar beets, characterized in that, The preparation steps include the following: S1. Inoculate the compound bacterial strains separately into liquid culture medium and culture them with shaking at 28-30℃ and 180-200rpm for 45-48h. After mixing, obtain a live bacterial solution. S2. By weight, add 5-8 parts of modified plant extract and 45-50 parts of modified carrier to 40-50 parts of modified reinforcing agent, and stir at 200-300 r / min for 15-20 min to obtain modified mixture; S3. Add the live bacteria solution obtained in step S1 to the modified mixture while stirring at a speed of 200-300 r / min, control the moisture content at 25-30%, then granulate using a disc granulator, and air dry at 38-40℃ for 6-8 hours to obtain the continuous cropping sugar beet soil conditioner. The preparation of the modified plant-derived extract includes the following steps: S11. By weight, mix 8-10 parts of Coptis chinensis powder and 8-10 parts of Sophora flavescens powder, add 180-200 parts of ethanol solution, and extract by stirring and reflux at 180-200 rpm for 1-2 hours under a water bath at 58-60℃. S12. Filter the solution obtained in step S11 using a Buchner funnel, add 130-150 parts of ethanol solution back to the filter residue, repeat the reflux extraction for 1-2 hours, and combine the filtrates obtained in step S11 and step S12. S13. Under conditions of 60℃ and -0.03MPa, the combined filtrates are concentrated to one-tenth of their original volume to obtain a thick extract; S14. Add 0.8-1 parts citric acid, 1.2-1.5 parts copper sulfate and 0.3-0.5 parts Tween-80 to the thick extract, and stir at 48-50℃ for 25-30 minutes to obtain the modified plant-derived extract.
2. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, The preparation of the modified carrier includes the following steps: S21. By weight, mix 20-25 parts of diatomaceous earth and 25-28 parts of biochar, calcine in a muffle furnace at 450-500℃ for 1-2 hours, cool and pass through a 100-mesh sieve to obtain the basic carrier. S22. Dissolve 0.8-1 part of glacial acetic acid in 35-40 parts of deionized water, slowly add 1.5-2 parts of chitosan powder, stir at 250-300 rpm for 1-2 hours in a 40℃ water bath until completely dissolved, then add 0.8-1 part of calcium nitrate and continue stirring until dissolved; S23. Add the base carrier obtained in step S21 to the mixture obtained in step S22, stir at 450-500 rpm, let it fully soak, let it stand at room temperature for 1-2 hours, dry it at 100-105℃ for 3-4 hours, and pulverize it through an 80-mesh sieve to obtain the modified carrier.
3. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, The preparation of the modified reinforcing agent includes the following steps: S31. By weight, mix 3-6 parts of polyglutamic acid with 5-8 parts of anhydrous ethanol and stir until dispersed to obtain a premix; S32. Add 15-18 parts of humic acid and 10-15 parts of sodium alginate to the premix, and stir at 30-50 rpm for 10-15 min to obtain the preliminary modifier. S33. Add 8-12 parts of potassium dihydrogen phosphate, 2-5 parts of zinc sulfate, and 4-7 parts of potassium humate to the preliminary modifier obtained in step S32 and stir at 30-50 rpm for 20-30 minutes to obtain the modified reinforcing agent.
4. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, The compound microbial strain consists of 5-7 parts of Bacillus subtilis, 3-5 parts of Trichoderma viride, 2-4 parts of Arthrobacter spheroidae, and 2-3 parts of Bacillus brevis.
5. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, In step S3, the particle size of the granulators produced by the disc granulator is controlled to be 2-4 mm.
6. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, The ethanol solution in steps S11 and S12 has a mass concentration of 70%.
7. The method for preparing the soil conditioner for continuously cropped sugar beets according to claim 1, characterized in that, The stirring speed in step S14 is 250-300 rpm.
8. A soil conditioner for continuously cropped sugar beets, characterized in that, The soil conditioner for continuous cropping of sugar beets is prepared by the preparation method described in any one of claims 1-7.
9. The application of the continuous cropping sugar beet soil conditioner according to claim 8 in sugar beet cultivation.