A method and agent for treating corn borer using a non-harmful physical method

Abstract

The present application relates to a kind of harmless physical method and reagent for treating corn borer.The reagent includes the following mass percentage components: colloid 0.2%-0.6%, diatomite 1%-2%, Bacillus thuringiensis 0.2%-0.3%, Beauveria bassiana 0.1%-0.15%, azadirachtin 0.03%-0.1%, and the balance is water.The present application uses the viscous colloid formed by colloid to effectively occupy corn bell mouth and stick to the corn borer that crawls out to feed, achieving physical control;At the same time, Bacillus thuringiensis and Beauveria bassiana biological components are used to infect and kill the corn borer, azadirachtin plays a role in repelling and growth inhibition, and diatomite can physically pierce the insect skin, enhancing the control effect, and through the synergistic effect of multiple components, biological control is achieved.The present application creatively uses harmless physical method combined with biological components to control corn borer, not only avoiding pesticide residues, but also being more environmentally friendly and safe, and synergistically enhancing the control effect, reducing the worm boring rate.

Description

Technical Field

[0001] This invention belongs to the field of crop pest and disease control technology, and relates to a method and reagent for harmless physical treatment of corn borers. Background Technology

[0002] The corn borer, also known as the corn stem borer, is a common pest in corn-producing areas worldwide, causing significant damage and leading to a substantial decrease in corn yield. Currently, the control of the corn borer mainly relies on chemical pesticides. However, the extensive use of chemical pesticides not only causes serious environmental pollution and disrupts the ecological balance, but also poses potential risks to human and animal health if corn containing chemical pesticide residues is consumed.

[0003] Existing control methods have many shortcomings. For example, some physical control methods are not long-lasting and are difficult to effectively stop the infestation of corn borers; some biological control methods, when used alone, are slow to take effect and cannot control the spread of pests in a timely manner. Therefore, developing a highly efficient, harmless, and environmentally friendly method for controlling corn borers and related reagents is of great practical significance. Summary of the Invention

[0004] To address the aforementioned technical problems, this invention provides a method and reagent for treating corn borers using a harmless physical method. This invention creatively combines a harmless physical method with biological components to control corn borers, not only avoiding environmental pollution and harm to human and animal health caused by chemical pesticides, making it more environmentally friendly and safer, but also synergistically enhancing the control effect and reducing the borer infestation rate.

[0005] The objective of this invention can be achieved through the following methods:

[0006] In a first aspect, the present invention provides a reagent for treating corn borers using a harmless physical method, comprising the following components by mass percentage: 0.2%-0.6% colloid, 1%-2% diatomaceous earth, 0.2%-0.3% Bacillus thuringiensis, 0.1%-0.15% Beauveria bassiana, 0.03%-0.1% azadirachtin, with the balance being water.

[0007] As one embodiment of the present invention, the colloid includes one or more of xanthan gum, guar gum, sodium carboxymethyl cellulose (CMC-Na), hydroxypropyl methyl cellulose (HPMC), and sodium alginate, and the mass percentage of the colloid in the reagent is 0.4%-0.6%.

[0008] As one embodiment of the present invention, the viable count of Bacillus thuringiensis is 10-20 billion CFU / g, and the viable count of Beauveria bassiana is 5-10 billion CFU / g.

[0009] As one embodiment of the present invention, the fineness of the diatomaceous earth is ≥300 mesh.

[0010] As one embodiment of the present invention, the reagent further includes a dispersing agent and a pH adjuster; the dispersing agent includes one or more of magnesium aluminum silicate and bentonite; the pH adjuster includes citric acid, adjusted to a pH of 6.0-6.5.

[0011] Furthermore, the dispersing agent has a mass percentage of 1%-3% in the reagent.

[0012] Secondly, the present invention provides a method for preparing a harmless physical method for treating corn borers, comprising the following steps: S1. Add the colloid to warm water and stir to mix; S2. Add dispersant, diatomaceous earth, Bacillus thuringiensis, and Beauveria bassiana in sequence, and stir to disperse. S3. Add azadirachtin and cold water, and add pH adjuster. Let it stand, and you will get the product.

[0013] The reagent used in this invention is a transparent viscous colloid.

[0014] In one embodiment of the present invention, in step S1 or S3, the temperature of the warm water is 40-50°C, and the temperature of the cold water is 0-4°C.

[0015] Thirdly, the present invention provides a method for treating corn borers using the reagent, comprising: spraying the reagent onto the core of corn at the whorl stage.

[0016] In one embodiment of the present invention, the spraying pressure is 15-25 bar. The amount of reagent used is 5-10 ml / plant.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention creatively uses a harmless physical method combined with biological components to control corn borers, which not only avoids the pollution of the environment and the harm to human and animal health caused by chemical pesticides, making it more environmentally friendly and safe, but also synergistically enhances the control effect and reduces the rate of insect infestation.

[0018] 2. The viscous colloid formed by the colloid in the reagent of this invention can effectively occupy the corn whorl opening, restrict the living space of the corn borer, prevent foreign corn borers from entering, prevent the corn borers hidden in the corn cob from crawling out to feed, and also cause the corn borers to suffocate, stick and starve to death through the action of the colloid.

[0019] 3. The diatomaceous earth in the reagent of this invention can physically pierce the insect skin, enhancing the control effect; biological components such as Bacillus thuringiensis and Beauveria bassiana can infect and kill borers; azadirachtin extract has repellent and growth-inhibiting effects; the synergistic effect of multiple components improves the effectiveness and persistence of control.

[0020] 4. The method of the present invention is simple to operate, and the preparation and application of reagents are easy to master, making it easy to promote and apply on a large scale. It can effectively reduce the damage caused by corn borers and improve corn yield and quality. Detailed Implementation

[0021] The present invention will now be described in detail with reference to specific embodiments. The following examples are implemented under the premise of the technical solution of the present invention, providing detailed implementation methods and specific operating procedures, which will help those skilled in the art to further understand the present invention. It should be noted that the scope of protection of the present invention is not limited to the following embodiments; any adjustments and improvements made under the concept of the present invention are all within the scope of protection of the present invention.

[0022] Materials list: Xanthan gum (food grade or industrial grade, purity ≥90%) Diatomaceous earth powder (300 mesh fineness); Bacillus thuringiensis powder (live bacteria count 100-200 billion CFU / g); Beauveria bassiana spore powder (50-100 billion CFU / g of live bacteria); Azadirachtin extract; Water (soft water); Dispersing agent (magnesium aluminum silicate); pH adjuster (citric acid).

[0023] Equipment list: Mixing bowl (with electric mixer, 1000 rpm); High-pressure sprayer (nozzle orifice diameter 1.2mm, anti-clogging design); Electronic scale (accurate to 0.1g).

[0024] Preparation of colloidal stock solution for reagents: a. Pour 70% of the total volume of warm water (40-50℃) into the mixing tank, start the stirrer (medium speed 800~1000 rpm), slowly sift in the xanthan gum (to avoid clumping), and continue stirring for 20-30 minutes until completely dissolved and the solution is transparent and viscous. b. Adjust the stirring speed to low (300-500 rpm), and add the dispersing agent, diatomaceous earth, Bacillus thuringiensis powder, and Beauveria bassiana spore powder in sequence. Stir for 2-5 minutes after each addition to ensure uniform particle dispersion. The dispersing agent should account for 1%-3% of the total mass of the reagent. c. Add azadirachtin extract and the remaining 30% cold water (0-4℃, to lower the overall temperature and protect microbial activity), and adjust the pH to 6.0-6.5 with a pH adjuster (if necessary to maintain the activity of the microbial agent). d. Stop stirring, let stand for 15 minutes to release air bubbles, check the viscosity, and use a stirring rod to lift the colloid. It should be able to form a continuous transparent string (viscosity meets the standard).

[0025] How to use: 1. Field application procedures Using a pressure sprayer, apply the pesticide after 4 PM on a sunny afternoon during the corn's trumpet-opening stage (when the heart leaves form a trumpet shape, approximately 6-8 leaves). The spray pressure should be 15-25 bar, and the nozzle should be directed vertically downwards towards the trumpet opening. The dosage per plant is 5-10 ml (until the heart leaves are fully filled and slightly overflowing). Maintain a constant walking speed and accurately inject the pesticide from a distance of 10-15 cm from the trumpet opening. The colloid should completely cover the inner wall and base of the heart leaves, forming a continuous viscous film (visually observed to be glossy, indicating it meets the standard).

[0026] 2. Post-application management The colloidal barrier lasts for 7-10 days (re-application is required if there is heavy rain); check the insect situation again after 7 days, and additional low-concentration Beauveria bassiana solution can be sprayed (to infect escaped larvae); stop using it 20 days before harvest.

[0027] Example 1 This embodiment provides a harmless physical method and reagents for treating corn borers, the reagents including: The mass percentages of the reagent components are as follows: xanthan gum 0.2%, diatomaceous earth 1%, Bacillus thuringiensis 0.2%, Beauveria bassiana 0.1%, azadirachtin 0.03%, magnesium aluminum silicate 1%, and the remainder is water.

[0028] Preparation of reagent colloid stock solution: a. Pour 70% of the total water volume into the mixing tank at 40℃, start the stirrer (medium speed 800 rpm), slowly sift in the xanthan gum, and continue stirring for 20 minutes until completely dissolved and the solution is transparent and viscous. b. Set the speed to low (300 rpm) and stir. Add magnesium aluminum silicate, diatomaceous earth, Bacillus thuringiensis powder, and Beauveria bassiana spore powder in sequence, stirring for 2 minutes after each addition. c. Add azadirachtin extract and the remaining 30% cold water (4°C), and adjust the pH to 6.0 with citric acid; d. Stop stirring, let stand for 15 minutes to release air bubbles, check the viscosity, and use a stirring rod to lift the colloid. If it forms a continuous transparent string, the viscosity meets the standard.

[0029] How to use: Field application procedure: Apply the pesticide on a sunny afternoon after 4 pm when the corn is in the trumpet opening stage (about 6 leaves). The spray pressure is 20 bar. The nozzle should be sprayed vertically from top to bottom towards the trumpet opening. The dosage per plant is 5 ml. Walk at a constant speed and spray the pesticide precisely 10 cm away from the trumpet opening. The colloid should completely cover the inner wall and base of the cotyledon, forming a continuous viscous film (visually glossy).

[0030] Post-application management: The colloidal barrier lasted for 7 days, during which no heavy rain occurred; after 7 days, a follow-up inspection of the insect situation was conducted, and no obvious insect situation was found, so no additional low-concentration Beauveria bassiana solution was sprayed; use was discontinued 20 days before harvest.

[0031] Example 2 This embodiment provides a harmless physical method and reagents for treating corn borers, including the following: The mass percentages of the reagent components are as follows: xanthan gum 0.4%, diatomaceous earth 1%, Bacillus thuringiensis 0.2%, Beauveria bassiana 0.1%, azadirachtin 0.03%, magnesium aluminum silicate 1%, and the remainder is water.

[0032] Preparation of reagent colloid stock solution: a. Pour 70% of the total water volume into the mixing tank at 40℃, start the stirrer (medium speed 800 rpm), slowly sift in the xanthan gum, and continue stirring for 20 minutes until completely dissolved and the solution is transparent and viscous. b. Adjust to low speed of 300 rpm and stir. Add magnesium aluminum silicate, diatomaceous earth, Bacillus thuringiensis powder and Beauveria bassiana spore powder in sequence, stirring for 5 minutes after each addition. c. Add azadirachtin extract and the remaining 30% cold water (4°C), and adjust the pH to 6.0 with citric acid; d. Stop stirring, let stand for 15 minutes to release air bubbles, check the viscosity, and use a stirring rod to lift the colloid. If it forms a continuous transparent string, the viscosity meets the standard.

[0033] How to use: Field application procedure: Apply the pesticide after 4 pm on a sunny day during the corn trumpet opening stage (approximately 6 leaves stage). The spray pressure is 15 bar. Spray the nozzle vertically from top to bottom towards the trumpet opening. The dosage per plant is 5 ml. Walk at a constant speed and spray the nozzle precisely 10 cm away from the trumpet opening. The colloid completely covers the inner wall and base of the cotyledon, forming a continuous viscous film (visually glossy).

[0034] Post-application management: The colloidal barrier lasted for 7 days, during which no heavy rain occurred; after 7 days, a follow-up inspection of the insect situation was conducted, and no obvious insect situation was found, so no additional low-concentration Beauveria bassiana solution was sprayed; use was discontinued 20 days before harvest.

[0035] Example 3 This embodiment provides a harmless physical method and reagents for treating corn borers, including: The mass percentages of the reagent components are as follows: xanthan gum 0.6%, diatomaceous earth 1%, Bacillus thuringiensis 0.2%, Beauveria bassiana 0.1%, azadirachtin 0.03%, magnesium aluminum silicate 1%, and the remainder is water.

[0036] Preparation of reagent colloid stock solution: a. Pour 70% of the total water volume into the mixing tank at 40℃, start the stirrer (medium speed 800 rpm), slowly sift in the xanthan gum, and continue stirring for 20 minutes until completely dissolved and the solution is transparent and viscous. b. Adjust to low speed of 300 rpm and stir. Add magnesium aluminum silicate, diatomaceous earth, Bacillus thuringiensis powder and Beauveria bassiana spore powder in sequence, stirring for 5 minutes after each addition. c. Add azadirachtin extract and the remaining 30% cold water (4°C), and adjust the pH to 6.0 with citric acid; d. Stop stirring, let stand for 15 minutes to release air bubbles, check the viscosity, and use a stirring rod to lift the colloid. If it forms a continuous transparent string, the viscosity meets the standard.

[0037] How to use: Field application procedure: Apply the pesticide after 4 pm on a sunny day during the corn trumpet opening stage (approximately 6 leaves stage). The spray pressure is 15 bar. Spray the nozzle vertically from top to bottom towards the trumpet opening. The dosage per plant is 5 ml. Walk at a constant speed and spray the nozzle precisely 10 cm away from the trumpet opening. The colloid completely covers the inner wall and base of the cotyledon, forming a continuous viscous film (visually glossy).

[0038] Post-application management: The colloidal barrier lasted for 7 days, during which no heavy rain occurred; after 7 days, a follow-up inspection of the insect situation was conducted, and no obvious insect situation was found, so no additional low-concentration Beauveria bassiana solution was sprayed; use was discontinued 20 days before harvest.

[0039] Comparative Example 1 This comparative example provides a method for treating corn borers, which involves spraying with a traditional chemical pesticide (10 ml / acre of 20% chlorantraniliprole suspension), following the instructions for use of conventional chemical pesticides.

[0040] Comparative Example 2 This comparative example provides a method for treating corn borers, using a xanthan gum-free reagent, with other components and treatment methods being the same as in Example 1.

[0041] Comparative Example 3 This comparative example provides a method for treating corn borers using xanthan gum colloid (0.60%) that does not contain diatomaceous earth, Bacillus thuringiensis, Beauveria bassiana, or azadirachtin extract. Other treatment methods are the same as in Example 1.

[0042] Effect verification: Examples 1-3 and Comparative Examples 1-3 were used for the control of corn stem borers. After a period of time, the borer infestation rate, yield, and pesticide residue levels of the corn were tested and compared. The specific testing methods are as follows: Insect infestation rate: The percentage of corn plants infested by corn borers out of the total number of plants.

[0043] Pesticide residue levels: Pesticide residues in corn are detected using professional testing equipment.

[0044] The test results for each example group and comparative example group are shown in Table 1.

[0045] Table 1

[0046] In Table 1, <LOQ indicates that no pesticide residues were detected.

[0047] According to the data in Table 1, as shown in Examples 1-3, the present invention uses a specific range of colloids (xanthan gum) that can reduce the rate of corn borer infestation, especially in the range of 0.4%-0.6%, where the rate of infestation is significantly reduced. Comparative Example 1 shows that traditional chemical pesticides have high pesticide residues, which are harmful to the environment and to human and animal health. Comparative Example 2 shows that without the use of xanthan gum, it is impossible to control corn borers through physical methods, leading to an increase in the rate of infestation. Comparative Example 3 shows that if xanthan gum is used alone, it is impossible to control corn borers through biological components, leading to a significant increase in the rate of infestation. Therefore, the present invention uses a harmless physical method combined with biological components to control corn borers, which avoids the environmental pollution and harm to human and animal health caused by chemical pesticides, avoids pesticide residues, and significantly improves the control effect, reducing the rate of infestation.

[0048] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A reagent for treating corn borers using a harmless physical method, characterized in that, It includes the following components by mass percentage: colloid 0.2%-0.6%, diatomaceous earth 1%-2%, Bacillus thuringiensis 0.2%-0.3%, Beauveria bassiana 0.1%-0.15%, azadirachtin 0.03%-0.1%, and the balance being water.

2. The reagent according to claim 1, characterized in that, The colloid includes one or more of xanthan gum, guar gum, sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, and sodium alginate, and the mass percentage of the colloid in the reagent is 0.4%-0.6%.

3. The reagent according to claim 1, characterized in that, The viable count of Bacillus thuringiensis is 10-20 billion CFU / g, and the viable count of Beauveria bassiana is 5-10 billion CFU / g; the fineness of the diatomaceous earth is ≥300 mesh.

4. The reagent according to claim 1, characterized in that, The reagent also includes a dispersing agent and a pH adjuster; the dispersing agent includes one or more of magnesium aluminum silicate and bentonite; the pH adjuster includes citric acid, adjusted to a pH of 6.0-6.

5.

5. The reagent according to claim 4, characterized in that, The dispersant has a mass percentage of 1%-3% in the reagent.

6. A method for preparing a reagent for treating corn borers using a harmless physical method as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Add the colloid to warm water and stir to mix; S2. Add dispersant, diatomaceous earth, Bacillus thuringiensis, and Beauveria bassiana in sequence, and stir to disperse. S3. Add azadirachtin and cold water, and add pH adjuster. Let it stand, and you will get the product.

7. The preparation method according to claim 6, characterized in that, In step S1 or S3, the temperature of the warm water is 40-50℃, and the temperature of the cold water is 0-4℃.

8. A method for treating corn borers using the reagent described in any one of claims 1-5, characterized in that, include: The reagent was sprayed onto the core of corn during the trumpet-opening stage.

9. The method according to claim 8, characterized in that, The pressure of the sprinkler is 15-25 bar.

10. The method according to claim 8, characterized in that, The amount of the reagent used is 5-10 ml per plant.

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