A fish minimally invasive intestinal flora sampling device

Abstract

The present application relates to the technical field of aquaculture detection, and provides a fish minimally invasive intestinal flora sampling device.The fish minimally invasive intestinal flora sampling device comprises a shell, a button, a push plate, a gas storage bag, a rotating shaft, a connecting frame, an anesthesia tube and a sampling tube;the top end of the shell is provided with the button, the inside of the shell is provided with the push plate, one side of the push plate is provided with the gas storage bag and a rebound spring, and the other side is connected with the button;one side of the shell is provided with the rotating shaft and the connecting frame, the connecting frame is provided with the anesthesia tube and the sampling tube at both ends;the shell is provided with an exhaust port and a gas release groove, the gas storage bag is magnetically sealed with the exhaust port, and the connecting frame is provided with a ventilation pipe and an exhaust pipe with a one-way valve.Thus, the problems of easy damage to the fish body, easy pollution of the sample, low sampling efficiency and inability to repeatedly sample of the existing device are solved, minimally invasive, efficient and non-polluting sampling is achieved, and the convenience and stability of the use of the device are further improved through a clear operation process.

Description

Technical Field

[0001] This invention relates to the field of aquaculture testing technology, specifically to a minimally invasive intestinal flora sampling device for fish. Background Technology

[0002] In aquaculture, aquatic biological research, and fish gut microbiota research, fish gut microbiota testing is a crucial indicator for assessing fish health, intestinal digestive function, and the aquatic ecological environment. Currently, the sampling method for fish gut microbiota mostly employs dissection sampling. Operators need to dissect the fish, open the intestines, and directly scrape the mucosa and microbiota samples from the intestinal wall. This method directly leads to fish death, making subsequent observation and rearing of live fish impossible. This is especially problematic for rare fry and research-grade tagged fish, resulting in sample loss and increased research and rearing costs.

[0003] Currently available non-invasive sampling tools mostly use ordinary soft tubes inserted directly into the intestine, relying on the adhesion of the tube wall for sampling. This results in problems such as insufficient sample volume, easy contamination of samples by bacteria on the body surface, and easy scratching of the intestinal mucosa by the hard tube opening. Some sampling tools that use negative pressure have no protective structure at the tube opening, which can easily scratch the intestinal wall during insertion, causing stress response in the fish and even causing intestinal damage, affecting the accuracy of sample test results, and also making it impossible to perform repeated sampling on the same fish.

[0004] Meanwhile, most existing sampling tools are of fixed size and cannot be adapted to fish of different body lengths and intestinal diameters, resulting in poor versatility. Furthermore, samples are prone to contamination of the tube wall and leakage after sampling, leading to low sample collection efficiency and making it difficult to meet the needs for efficient, minimally invasive, and pollution-free fish intestinal flora sampling. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a minimally invasive intestinal flora sampling device for fish, which solves the technical problems of easy damage to the fish body, easy sample contamination, low sampling efficiency, and inability to repeat sampling in existing technologies.

[0006] The technical solution adopted in this invention is a minimally invasive intestinal flora sampling device for fish, comprising a shell, a button, a push plate, an air reservoir, a rotating shaft, a connecting frame, an anesthesia component, and a sampling component; The button is mounted on the top of the housing, the push plate is slidably fitted inside the housing, and the air reservoir is located on the side of the push plate away from the button. The outer casing has an exhaust port and an air vent on the side away from the button. The rotating shaft is fixed to the side of the outer casing away from the button, and the connecting bracket is rotatably mounted on the end of the rotating shaft away from the outer casing. The anesthesia component and the sampling component are symmetrically arranged at both ends of the connecting frame on the side away from the rotation axis. The anesthesia tube and the sampling tube are both provided with limiting vent holes, and both are equipped with limiting vent frames.

[0007] Furthermore, one end of the button is fixedly connected to the center of one side of the push plate. Pressing the button can drive the push plate to move towards the side closer to the air bladder. A rebound spring is installed between the push plate and the outer shell. A positioning frame is fixed on the side of the push plate away from the button. The positioning frame wraps around the outside of the air bladder and limits and fixes it.

[0008] Furthermore, the output end of the air reservoir is provided with a cover, and a fixed magnetic bracket is fixed inside the exhaust port. The fixed magnetic bracket and the cover are sealed together by magnetic attraction. When the button is pressed, the fixed magnetic bracket can open the cover, allowing the compressed gas in the air reservoir to be discharged.

[0009] Furthermore, rotating the rotating shaft can adjust the tilt angle of the connecting frame so that the anesthesia tube or sampling tube is accurately aligned with the exhaust port; two ventilation tubes are fixed on the side of the connecting frame near the outer shell, and the two ventilation tubes are respectively connected to the anesthesia tube and the sampling tube.

[0010] Furthermore, both ventilation tubes are equipped with one-way valves, and exhaust pipes are fixed to the sides of the two ventilation tubes respectively. The diameter of the exhaust pipes is adapted to the width of the venting groove. When the anesthesia tube or sampling tube is aligned with the exhaust port, the exhaust pipe on the corresponding other side can be embedded in the venting groove to discharge excess compressed gas in the tube.

[0011] Furthermore, a first pushing ring is slidably fitted inside the end of the anesthesia tube near the connecting frame. The first pushing ring has a through hole in its center. A limiting plate is fixed to the center of the side of the first pushing ring near the connecting frame. The diameter of the limiting plate is larger than the diameter of the through hole in the center of the first pushing ring. An extension frame is fixed to the side of the limiting plate away from the first pushing ring. The end of the extension frame away from the limiting plate extends through the first pushing ring into the anesthesia tube, and the diameter of the extension frame is smaller than the diameter of the through hole in the center of the first pushing ring.

[0012] Furthermore, a needle tube is fixed inside the extension frame, and a pressure cone is slidably fitted inside the needle tube. A needle tip is fixed at the center of the end of the needle tube away from the extension frame. A sealing ring is fixed inside the end of the anesthesia tube away from the connecting frame. The end of the needle tip away from the needle tube extends through the sealing ring to the outside of the anesthesia tube. A first limiting ring is fixed on the side of the sealing ring near the needle tube. The inner diameter of the first limiting ring is the same as the diameter of the extension frame. A plurality of first return springs are evenly fixed between the first pushing ring and the first limiting ring.

[0013] Furthermore, a second pushing ring is slidably fitted inside the sampling tube near the connecting frame. A rotating screw is fixed at the center of the side of the second pushing ring away from the connecting frame. A sampling swab is threadedly connected to the end of the rotating screw away from the second pushing ring. A rotating frame is fixed inside the sampling tube near the sampling swab. An internal thread adapted to the rotating screw is opened on the inner wall of the rotating frame. A positioning and fixing frame is fixed at the end of the sampling tube away from the connecting frame.

[0014] Furthermore, a second limiting ring is fixed between the rotating frame and the second pushing ring, and a plurality of second return springs are uniformly fixed between the second limiting ring and the second pushing ring; a connecting thread is provided on the outer surface of the sampling swab near the rotating screw, and the connecting thread is adapted to the external thread of the rotating screw.

[0015] Furthermore, the limiting vent hole in the anesthesia tube is opened between the first limiting ring and the first pushing ring, and the limiting vent hole in the sampling tube is opened between the second limiting ring and the rotating frame; the limiting vent frame is fixed to the side of the first pushing ring near the needle tube and the side of the second pushing ring near the rotating frame, respectively, and a through hole is opened inside the limiting vent frame, the diameter of the through hole being the same as the diameter of the limiting vent hole.

[0016] As can be seen from the above technical solution, the beneficial technical effects of the present invention are as follows: 1. The present invention provides a minimally invasive intestinal flora sampling device for fish. By using an anesthesia tube and a sampling tube together, the fish are first anesthetized and then the sampling operation is performed. The protective structure of the anesthesia tube and the sampling tube avoids scratching the intestinal wall, thus achieving minimally invasive sampling. Live fish can be sampled repeatedly, reducing sample fish loss and lowering scientific research and breeding costs.

[0017] 2. The present invention provides a minimally invasive intestinal flora sampling device for fish. The angle of the connecting frame can be adjusted by rotating the shaft to adapt to the sampling needs of fish of different body lengths. The sampling swab can be quickly disassembled and replaced by threads to avoid cross-contamination of samples. The trumpet-shaped design and smooth transition structure of the positioning and fixing frame further improve the convenience of sampling and the minimally invasive effect. It is suitable for fish with different intestinal diameters and has strong versatility.

[0018] 3. The fish minimally invasive intestinal flora sampling device provided by the present invention realizes the automatic reset of each push ring through the rebound spring and return spring, which simplifies the operation process and improves the convenience of sampling. At the same time, the limiting vent hole and the limiting vent frame cooperate to balance the air pressure in the tube, avoid excessive air pressure causing intestinal damage or device malfunction, and further improve the stability and reliability of the device. Attached Figure Description

[0019] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0020] Figure 1 This is a schematic diagram of the structure of a minimally invasive intestinal flora sampling device for fish according to the present invention; Figure 2 This is a front view of a minimally invasive intestinal flora sampling device for fish according to the present invention; Figure 3 This is a cross-sectional structural diagram of a minimally invasive intestinal flora sampling device for fish according to the present invention; Figure 4 For the present invention Figure 3 The front view; Figure 5 This is a schematic diagram of the structure of the magnetic clamp and the cover of the present invention; Figure 6 This is a schematic diagram of the internal structure of the anesthesia tube of the present invention; Figure 7 For the present invention Figure 6 The front view; Figure 8 This is a schematic diagram of the sampling tube of the present invention; Figure 9 This is a cross-sectional view of the sampling tube of the present invention.

[0021] Figure label: 1-Outer shell, 101-Exhaust port, 102-Vent groove, 2-Button, 3-Push plate, 301-Positioning frame, 4-Rebound spring, 5-Air reservoir, 501-Fixing magnetic bracket, 502-Cap, 6-Rotating shaft, 7-Connecting frame, 701-Ventilation tube, 702-One-way valve, 703-Exhaust pipe, 8-Anesthetic tube, 801-First push ring, 802-Limiting plate, 803-Extension frame, 804-Needle, 805 - Pressure cone, 806 - Needle, 807 - First limiting ring, 808 - First return spring, 809 - Sealing ring, 9 - Sampling tube, 901 - Second pushing ring, 902 - Rotating screw, 903 - Second limiting ring, 904 - Second return spring, 905 - Rotating frame, 906 - Connecting thread, 907 - Sampling swab, 908 - Positioning and fixing frame, 10 - Limiting vent hole, 11 - Limiting vent frame, 1101 - Through hole. Detailed Implementation

[0022] The embodiments of the technical solution of the present invention will now be described in detail with reference to the accompanying drawings. These embodiments are merely illustrative of the technical solution of the present invention and are therefore intended to limit the scope of protection of the present invention.

[0023] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0024] like Figures 1-9 As shown in the figure, the fish minimally invasive intestinal flora sampling device provided in this embodiment is an integrated minimally invasive sampling structure, which is specially designed for fish intestinal flora sampling. It takes into account the four core requirements of minimally invasive, efficient, pollution-free and reusable sampling. Combined with a clear usage procedure, it solves the defects of traditional sampling devices that are easy to damage fish, easy to contaminate samples, poor versatility, inconvenient to operate and easy to be damaged. It is suitable for aquaculture, aquatic biological research and other scenarios.

[0025] In this embodiment, a minimally invasive intestinal flora sampling device for fish includes: a housing 1 and a rotating shaft 6 disposed on the housing 1, a pneumatic mechanism disposed inside the housing 1, a connecting frame 7 rotatably disposed on the rotating shaft 6, and anesthesia tubes 8 and sampling tubes 9 disposed at both ends of the connecting frame 7; wherein, the pneumatic mechanism serves as the power core, used to provide compressed gas to drive the anesthesia component and the sampling component to complete corresponding operations, and its specific structure includes a button 2, a push plate 3, an air reservoir 5 and a rebound spring 4, with each component working together to achieve power output.

[0026] Specifically, button 2 is fixed to the top of housing 1, and push plate 3 is slidably disposed inside housing 1. One end of button 2 is fixed to the center of one side of push plate 3. Pressing button 2 can drive push plate 3 to slide along the inside of housing 1 towards the side closer to air bladder 5. Air bladder 5 is fixed to the side of push plate 3 away from button 2. A positioning frame 301 is fixed to the side of push plate 3 away from button 2. The positioning frame 301 is set to wrap around air bladder 5 to fix air bladder 5 and prevent it from shifting. Spring 4 is fixed between push plate 3 and housing 1 for automatic reset of push plate 3. After button 2 is released, spring 4 can drive push plate 3 to reset.

[0027] The outer casing 1 provides a mounting carrier and protection for the entire device. The button 2 is used to control the sliding of the push plate 3, thereby controlling the compression and exhaust of the gas reservoir 5. The push plate 3 is used to compress the gas reservoir 5, delivering the gas inside the gas reservoir 5 to the ventilation tube 701. The gas reservoir 5 is used to store gas, providing power for the release of anesthetic and sampling operations. The rebound spring 4 realizes the automatic reset of the push plate 3, improving the ease of operation. The positioning frame 301 is a ring-shaped elastic wear-resistant structure with its inner wall tightly fitted to the gas reservoir 5. It can both fix the gas reservoir 5 and adapt to the expansion and contraction of the gas reservoir 5, protecting the gas reservoir 5 from damage.

[0028] The outer casing 1 has an exhaust port 101 and a venting groove 102 on the side away from the button 2 (i.e., the bottom). The exhaust port 101 is used for the air bladder 5 to vent and inflate, and the venting groove 102 is used for the exhaust pipe 703 to vent and adjust its position. The output end of the air bladder 5 is fixed with a cover 502, and a fixed magnetic bracket 501 is fixed inside the exhaust port 101. The fixed magnetic bracket 501 is magnetically connected to the cover 502. When the button 2 is pressed, the push plate 3 squeezes the air bladder 5, and the compressed gas in the air bladder 5 can push open the cover 502 which is magnetically connected to the fixed magnetic bracket 501, so that the gas can be discharged.

[0029] In another embodiment, the rotating shaft 6 is fixed at the bottom of the outer shell 1, between the exhaust port 101 and the venting groove 102. The connecting frame 7 is rotatably disposed at the end of the rotating shaft 6 away from the outer shell 1. The rotating shaft 6 can drive the connecting frame 7 to rotate, thereby adjusting the angle of the anesthesia tube 8 and the sampling tube 9 so that the anesthesia tube 8 or the sampling tube 9 is aligned with the exhaust port 101, adapting to the sampling needs of fish of different body lengths. The anesthesia tube 8 and the sampling tube 9 are respectively fixed at both ends of the connecting frame 7 on the side away from the rotating shaft 6. The anesthesia tube 8 is used to inject an anesthetic locally into the fish's intestine, and the sampling tube 9 is used to collect intestinal flora samples. The two work together to achieve minimally invasive sampling.

[0030] Two ventilation tubes 701 are fixed on the side of the connecting frame 7 near the outer shell 1. The two ventilation tubes 701 are connected to the anesthesia tube 8 and the sampling tube 9 respectively, and are used to deliver the compressed gas discharged from the gas reservoir 5 to the anesthesia tube 8 and the sampling tube 9. One-way valves 702 are fixed inside each of the two ventilation tubes 701. The one-way valves 702 are directed from the gas reservoir 5 to the ventilation tube 701, which prevents backflow of gas and ensures a stable delivery of gas from the gas reservoir 5 to the anesthesia tube 8 and the sampling tube 9. An exhaust pipe 703 is fixed to each side of the ventilation tube 701. The diameter of the exhaust pipe 703 is the same as the width of the venting groove 102. The exhaust pipe 703 can slide in the venting groove 102. When the anesthesia tube 8 or the sampling tube 9 is aligned with the exhaust port 101, the exhaust pipe 703 on the other side moves into the venting groove 102 to discharge excess gas in the ventilation tube 701 and the corresponding tube (anesthesia tube 8 or sampling tube 9), adjust the air pressure in the tube, and achieve component reset with the help of the return spring.

[0031] A first pushing ring 801 is slidably disposed inside the end of the anesthesia tube 8 near the connecting frame 7. The first pushing ring 801 has a hole in its center. A limiting plate 802 is fixed to the center of the side of the first pushing ring 801 near the connecting frame 7. The diameter of the limiting plate 802 is larger than the central hole of the first pushing ring 801, which can prevent the first pushing ring 801 from sliding excessively and ensure structural stability. An extension frame 803 is fixed to the side of the limiting plate 802 away from the first pushing ring 801. The end of the extension frame 803 away from the limiting plate 802 passes through the first pushing ring 801 and extends into the anesthesia tube 8. The diameter of the extension frame 803 is smaller than the diameter of the hole in the first pushing ring 801, which facilitates the sliding of the extension frame 803.

[0032] The first push ring 801 can slide along the inner wall of the anesthesia tube 8, driving the extension frame 803 to slide synchronously, thereby realizing the extension and retraction of the needle 806; the limiting plate 802 can limit the sliding stroke of the first push ring 801, preventing the first push ring 801 from leaving the inside of the anesthesia tube 8, improving structural stability. At the same time, when the extension frame 803 contacts the first limiting ring 807, it can limit the extension frame 803 from continuing to move, allowing the first push ring 801 to drive the needle tube 804 to move independently.

[0033] An extension frame 803 has a needle tube 804 fixed inside. A pressure cone 805 is slidably disposed inside the needle tube 804, sliding along the inner wall of the needle tube 804 to compress the anesthetic inside, achieving a quantitative release of the anesthetic. A needle tip 806 is fixed at the center of the end of the needle tube 804 furthest from the extension frame 803. The needle tip 806 is used to inject the anesthetic into the intestine. A sealing ring 809 is fixed inside the end of the anesthesia tube 8 furthest from the connecting frame 7. The end of the needle tip 806 furthest from the needle tube 804 extends through the sealing ring 809 to the outside of the anesthesia tube 8. The sealing ring 809 ensures the airtightness of the anesthesia tube 8, preventing anesthetic leakage. The side of the sealing ring 809 closest to the needle tube 804 is fixed... A first limiting ring 807 is fixed, the inner diameter of which is the same as the diameter of the extension frame 803. This ring is used to limit the sliding direction of the extension frame 803 and prevent it from deviating. At the same time, when the end of the extension frame 803 away from the limiting plate 802 contacts the first limiting ring 807, the limiting plate 802 remains fixed, and the first pushing ring 801 can continue to move and drive the needle tube 804 to move until the end of the needle tube 804 near the needle tip 806 contacts the sealing ring 809. Several first return springs 808 are evenly fixed between the first pushing ring 801 and the first limiting ring 807. The first return springs 808 can realize the automatic reset of the first pushing ring 801, which is convenient for repeated use.

[0034] A second pushing ring 901 is slidably disposed inside the sampling tube 9 near the connecting frame 7. A rotating screw 902 is fixed at the center of the side of the second pushing ring 901 away from the connecting frame 7. A sampling swab 907 is threadedly connected to the end of the rotating screw 902 away from the second pushing ring 901. The sampling swab 907 is used to scrape mucosal and bacterial samples from the intestinal wall. The threaded connection design facilitates quick disassembly and replacement, avoiding cross-contamination of samples. A rotating frame 905 is fixed inside the sampling tube 9 near the sampling swab 907. The inner wall of the rotating frame 905 is provided with an internal thread that matches the rotating screw 902. The rotating screw 902 cooperates with the rotating frame 905 to realize the extension and rotation of the sampling swab 907, which is convenient for deep intestinal sampling. The sampling tube 9 is fixed with a positioning and fixing frame 908 at the end away from the connecting frame 7. The positioning and fixing frame 908 has a trumpet-shaped structure, and its end away from the sampling tube 9 is smoothly transitioned, which can reduce the resistance when the sampling tube 9 is inserted into the intestine, avoid scratching the intestine, improve the minimally invasive effect, and at the same time facilitate the alignment with the fish's excretory opening.

[0035] In another embodiment, a second limiting ring 903 is fixed between the rotating frame 905 and the second pushing ring 901. The second limiting ring 903 is used to limit the sliding range of the second pushing ring 901 and prevent it from sliding excessively. A plurality of second return springs 904 are evenly fixed between the second limiting ring 903 and the second pushing ring 901. The second return springs 904 are used to realize the automatic reset of the second pushing ring 901. A connecting thread 906 is provided on the outer surface of the end of the sampling swab 907 near the rotating screw 902. The connecting thread 906 is adapted to the external thread of the rotating screw 902 to ensure that the sampling swab 907 is firmly connected to the rotating screw 902 and to prevent it from falling off during sampling. After sampling is completed, the sampling swab 907 can be rotated to disassemble it through the connecting thread 906 and a new sampling swab 907 can be replaced for the next sampling.

[0036] Both the anesthesia tube 8 and the sampling tube 9 have limiting vent holes 10 inside. The limiting vent hole 10 near the anesthesia tube 8 is located between the first limiting ring 807 and the first pushing ring 801, and the limiting vent hole 10 near the sampling tube 9 is located between the second limiting ring 903 and the rotating frame 905. Limiting vent frames 11 are fixed on the side of the first pushing ring 801 near the needle tube 804 and the side of the second pushing ring 901 near the second limiting ring 903. The limiting vent frame 11 has a through hole 1101 inside, and the diameter of the through hole 1101 is the same as the diameter of the limiting vent hole 10.

[0037] The limiting vent 10 and the limiting vent bracket 11 work together to balance the air pressure inside the pipe, preventing excessively high or low air pressure from affecting the operation of the components. At the same time, when the first push ring 801 or the second push ring 901 moves down to a certain distance, the through hole 1101 overlaps with the limiting vent 10, venting excess compressed gas and preventing the device from breaking or malfunctioning, further improving the safety and stability of the device.

[0038] In addition, the ventilation tube 701 and the air reservoir 5 are sealed together by a flexible tube to prevent gas leakage and facilitate the rotation of the connecting frame 7; the end of the sampling swab 907 away from the rotating screw 902 is a flexible brush structure to avoid scratching the intestinal wall and to fully scrape the sample, improving the sampling volume and sample integrity; the one-way valve 702 can prevent gas backflow and ensure stable airflow transmission; the elastic and wear-resistant material of the positioning frame 301 can protect the air reservoir 5 and extend the service life of the device.

[0039] The specific operating procedure for this minimally invasive intestinal flora sampling device for fish is as follows: 1. Anesthesia Operation: The operator holds the device, rotates the rotating shaft 6, aligns the end of the connecting frame 7 near the anesthesia tube 8 with the exhaust port 101, and aligns the needle 806 of the anesthesia tube 8 with the gills of the fish; presses button 2, which pushes the push plate 3 towards the air reservoir 5, causing the push plate 3 to compress the air reservoir 5. The cap 502 at the output end of the air reservoir 5 is opened by the fixed magnetic suction frame 501, and compressed gas enters the ventilation tube 701 from the air reservoir 5, and then enters the anesthesia tube 8 through the ventilation tube 701; the compressed gas pushes the first push ring 801 to move away from the connecting frame 7, from... The limiting plate 802, extension frame 803, syringe 804, and needle 806 move synchronously. When the end of the extension frame 803 away from the limiting plate 802 contacts the first limiting ring 807, the limiting plate 802 remains stationary. The compressed gas continues to push the first pushing ring 801, thereby driving the syringe 804 to move continuously away from the connecting frame 7. When the end of the syringe 804 near the needle 806 contacts the sealing ring 809, the compressed gas continues to be injected and pushes the pressure cone 805, injecting the anesthetic in the syringe 804 into the fish's body to complete the anesthesia.

[0040] 2. Reset of the anesthesia tube: After the anesthetic is injected, the needle 806 is removed from the fish, and the rotating shaft 6 is rotated to align the sampling tube 9 with the exhaust port 101. At this time, the exhaust pipe 703 on the side near the anesthesia tube 8 moves into the venting groove 102. The compressed gas inside the anesthesia tube 8 is slowly discharged from the exhaust pipe 703 under the elastic force of the first return spring 808, which in turn drives the first push ring 801, the limiting plate 802, the extension frame 803, the needle tube 804 and the needle 806 to reset, and the internal structure of the anesthesia tube 8 returns to its original position.

[0041] 3. Sampling Operation: Align the positioning and fixing frame 908 and the sampling swab 907 with the fish's excretory opening, press button 2 again, and the air bladder 5 releases compressed gas under the pressure of the push plate 3. The compressed gas enters the sampling tube 9 through the vent pipe 701. The compressed gas pushes the second push ring 901 to apply pressure to the sampling swab 907, causing the rotating screw 902 to move downward. At the same time, due to the presence of the rotating frame 905, the rotating screw 902 rotates continuously during the downward movement, thereby causing the sampling swab 907 to rotate slowly and extend into the fish's intestine for bacterial sampling. During this process, if the air pressure in the sampling tube 9 is too high, when the second push ring 901 moves down a certain distance, the through hole 1101 on the limiting exhaust frame 11 overlaps with the limiting exhaust hole 10, and the excess compressed gas is discharged to prevent the device from malfunctioning.

[0042] 4. Post-sampling processing: After sampling, pull the sampling swab 907 out of the fish's excretory opening, rotate the sampling swab 907, and use the connecting thread 906 to remove the sampling swab 907 from the rotating screw 902. Replace it with a new sampling swab 907 and prepare for sampling the next fish.

[0043] 5. Sampling tube reset: Similarly, when the rotating shaft 6 is rotated again and the anesthesia tube 8 is aligned with the exhaust port 101, the exhaust pipe 703 on the side near the sampling tube 9 will enter the venting groove 102. Under the elastic force of the second return spring 904, the compressed gas in the sampling tube 9 is discharged from the exhaust pipe 703, which drives the second push ring 901, the rotating screw 902 and the new sampling swab 907 to reset, and the internal structure of the sampling tube 9 returns to its original position.

[0044] It should be noted that the components involved in this invention, such as the air reservoir 5, the one-way valve 702, and the sampling swab 907, are all commercially available and mature components in the prior art, and are not improvements of this invention. Among them, the air reservoir 5 can be a medical-grade elastic air reservoir, the one-way valve 702 can be a miniature plastic one-way valve, and the sampling swab 907 can be a sterile flexible brush swab. Those skilled in the art can select and adapt the appropriate components according to actual usage requirements.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. A minimally invasive intestinal flora sampling device for fish, characterized in that, include: The outer casing (1), pneumatic mechanism, rotating shaft (6), connecting frame (7), anesthesia assembly and sampling assembly; The bottom of the outer shell (1) is provided with an exhaust port (101) and an air release groove (102), and the middle part of the outer shell (1) of the exhaust port (101) and the air release groove (102) is connected to the rotating shaft (6). One side of the connecting frame (7) is connected to the rotating shaft (6), and the other side is connected to the anesthesia component and the sampling component respectively; rotating the connecting frame (7) can make the anesthesia component or the sampling component communicate with the exhaust port (101) or the venting groove (102) respectively; The pneumatic mechanism is housed within the housing (1), and its outlet is connected to the exhaust port (101) to provide compressed gas to the exhaust port (101) when the pneumatic mechanism is activated, thereby driving the connected: The needle (806) of the anesthesia component is ejected; or The sampling swab (907) of the sampling component pops out.

2. The fish minimally invasive intestinal flora sampling device according to claim 1, characterized in that, The pneumatic mechanism includes a button (2), a push plate (3), an air reservoir (5), and a return spring (4); the button (2) is installed on the top of the housing (1), one end of which is fixedly connected to the center of one side of the push plate (3), the push plate (3) is slidably assembled inside the housing (1), and the return spring (4) is assembled between the push plate (3) and the housing (1) to reset the button (2); the air reservoir (5) is disposed inside the housing (1) below the push plate (3), and a positioning frame (301) for wrapping and fixing the air reservoir (5) is provided on the side of the push plate near the air reservoir (5), and the outlet end of the air reservoir (5) is connected to the exhaust port (101) through a switch assembly; pressing the button (2) can cause the switch assembly to open the outlet of the air reservoir (5).

3. The fish minimally invasive intestinal flora sampling device according to claim 2, characterized in that, The switch assembly includes a cover (502) and a fixed magnetic holder (501). The cover (502) is located at the output end of the gas storage bag (5), and the fixed magnetic holder (501) is located inside the exhaust port (101). The fixed magnetic holder (501) and the cover (502) are connected by magnetic attraction. When the button (2) is pressed, the fixed magnetic holder (501) can open the cover (502), so that the compressed gas in the gas storage bag (5) can be injected into the sampling tube (9) or the anesthesia tube (8) through the exhaust port (101).

4. The fish minimally invasive intestinal flora sampling device according to claim 2, characterized in that, The connecting frame (7) is fixedly provided with two ventilation tubes (701) on the side near the outer shell (1). The two ventilation tubes (701) are respectively connected to the anesthesia component and the sampling component, and both ventilation tubes (701) are equipped with a one-way valve (702) that allows gas to flow outward from the gas storage bag (5).

5. The fish minimally invasive intestinal flora sampling device according to claim 4, characterized in that, Two ventilation tubes (701) are respectively fixed with exhaust pipes (703) on their sides. The diameter of the exhaust pipe (703) is adapted to the width of the venting groove (102). When the anesthesia tube (8) or the sampling tube (9) is aligned with the exhaust port (101), the exhaust pipe (703) on the other side can be embedded in the venting groove (102) to discharge excess compressed gas in the anesthesia assembly or the sampling assembly.

6. The fish minimally invasive intestinal flora sampling device according to claim 1, characterized in that, The anesthesia assembly includes: an anesthesia tube (8) connected to the connecting frame (7), with a first pushing ring (801) slidably fitted inside the end of the anesthesia tube (8) near the connecting frame (7). The first pushing ring (801) has a through hole in its center, and a limiting plate (802) is fixed at the center of its side near the connecting frame (7). The diameter of the limiting plate (802) is larger than the diameter of the through hole of the first pushing ring (801) to prevent the limiting plate (802) from passing through the first pushing ring (801). The limiting plate (802) passes through the first... An extension frame (803) is provided in the through hole of the push ring (801) and is located inside the anesthesia tube (8); a first limiting ring (807) is fixed inside the anesthesia tube (8) at the end of the extension frame (803) away from the first push ring (801) to limit the sliding range of the extension frame (803) in the anesthesia tube (8); a plurality of first return springs (808) are uniformly fixed between the first push ring (801) and the first limiting ring (807) to reset the various components inside the anesthesia tube (8) after the drug injection is completed.

7. The fish minimally invasive intestinal flora sampling device according to claim 6, characterized in that, The extension frame (803) is equipped with a needle tube (804) inside. A needle tip (806) is provided at the center of the end of the needle tube (804) away from the extension frame (803). A pressure cone (805) is slidably fitted inside the needle tube (804) to push the medicine in the needle tube (804) through the needle tip (806) and inject it into the fish. A sealing ring (809) is fixed inside the end of the anesthesia tube (8) away from the connecting frame (7). The needle tip (806) passes through the sealing ring (809) and cooperates with the sealing ring (809) to seal the anesthesia tube (8).

8. The fish minimally invasive intestinal flora sampling device according to claim 1, characterized in that, The sampling assembly includes: a sampling tube (9) connected to the connecting frame (7), a second push ring (901) is slidably fitted inside the sampling tube (9) near the connecting frame (7), a rotating screw (902) is rotated on the side of the second push ring (901) away from the connecting frame (7), a sampling swab (907) is screwed to the end of the rotating screw (902) away from the second push ring (901), a connecting thread (906) is opened on one end of the sampling swab (907), and the connecting thread (906) is adapted to the external thread of the rotating screw (902); a rotating frame (905) is fixed inside the sampling tube (9) near the sampling swab (907). When compressed gas is injected and presses down the second push ring (901), the rotating frame (905) will drive the rotating screw (902) and the sampling swab (907) to rotate along the thread direction.

9. The fish minimally invasive intestinal flora sampling device according to claim 8, characterized in that, A second limiting ring (903) is fixed between the rotating frame (905) and the second pushing ring (901), and a plurality of second return springs (904) are evenly fixed between the second limiting ring (903) and the second pushing ring (901); a positioning fixing frame (908) for positioning the sampling tube (9) away from the connecting frame (7) is fixed at one end.

10. The fish minimally invasive intestinal flora sampling device according to claim 1, characterized in that, Both the anesthesia component and the sampling component have a limiting vent hole (10) inside, and both are equipped with a limiting vent frame (11); the limiting vent frame (11) has a through hole (1101) inside, and when the through hole (1101) and the limiting vent hole (10) are at the same horizontal line, excess compressed gas in the sampling component or the anesthesia component will be released.

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