Use of rhamnolipids in surfactant systems

Abstract

The present invention relates to the use of rhamnolipids to impart a feeling of mildness on the hands to consumers in surfactant systems for hand washing detergents.

Description

[0001] This application is a divisional application of Chinese invention patent application filed on July 11, 2019, with application number 201980047655.5 and invention title "Use of rhamnolipin in surfactant systems". Technical Field

[0002] This invention relates to the use of rhamnolipids in surfactant systems for handwashing detergents. Background Technology

[0003] Hand wash detergents are detergents that involve consumers washing the substrate with their hands. Applications primarily include laundry (i.e., hand washing of clothes) and hand dishwashing (HDW) (i.e., hand washing of dishes, etc.). Hand wash detergents involve close contact between the detergent liquid and the hands during the washing process, whether in laundry or HDW. While formulation efficacy is an important attribute for restoring consumer experience, the sensory experience of the formulation and its use by the consumer during and after washing leave a lasting impression of whether the consumer has a good or bad experience with the product.

[0004] Sensory experiences can include, for example, a pleasant color or scent of the product itself or during use. Another important sensory experience for consumers using handwashing detergents is the lingering feeling on their hands. This is important because the handwashing process involves the consumer's hands coming into contact with the formulation and the resulting detergent solution, and the formulation may irritate the skin.

[0005] An improved handwashing detergent is needed that leaves an improved feeling on the hands. In particular, those that leave an improved feeling of gentleness on the hands during the washing process, and a lasting feeling of gentleness on the hands after the handwashing process is finished.

[0006] One way to address this is to include ingredients that enhance gentleness, such as polyethylene glycol. Summary of the Invention

[0007] We have found that the use of rhamnolipids in surfactant systems for handwashing detergents imparts a gentle feel to consumers' hands. By using rhamnolipids, the need for ingredients such as polyethylene glycol is reduced or eliminated.

[0008] The invention relates in a first aspect to the use of rhamnolipin in surfactant systems for handwashing detergents to impart a mild feeling to the hands of consumers.

[0009] Preferably, the gentle feeling on the hands persists after the hand-washing process is completed and the hands have dried.

[0010] Preferably, in terms of use, rhamnolipid exists in the surfactant system at a content of 5-100% by weight of the surfactant system, preferably 10-90% by weight, more preferably 15-80% by weight, and most preferably 15-50% by weight.

[0011] Preferably, the handwashing detergent is a fluid detergent composition, and more preferably an aqueous detergent composition.

[0012] Preferably, rhamnolipin is present in the composition at a content of 1-20% by weight, more preferably 1.25-15% by weight, more preferably 1.5-12.5% ​​by weight, and most preferably 2-10% by weight.

[0013] Preferably, the handwashing detergent is a manual dishwashing composition or a liquid laundry detergent composition for handwashing.

[0014] Preferably, the rhamnose glycolipid contains at least 50% by weight of dirhamnose glycolipid, more preferably at least 60% by weight of dirhamnose glycolipid, even more preferably 70% by weight of dirhamnose glycolipid, and most preferably at least 80% by weight of dirhamnose glycolipid.

[0015] Preferably, the rhamnose glycolipid is of formula Rha2C. 8-12 C 8-12 The two rhamnolipids.

[0016] Any preferred subject matter described herein may be combined with any other subject matter, in particular, two or more preferred subject matters.

[0017] This article also covers the following items: 1. The use of rhamnolipids in surfactant systems for handwashing detergents to impart a mild feeling to consumers' hands.

[0018] 2. As described in Project 1, the mild sensation on the hands persists after the hand-washing process has ended and the hands have dried.

[0019] 3. According to the use described in Project 1 or Project 2, wherein the rhamnolipin is present in the surfactant system at a content of 5-100% by weight, preferably 10-90% by weight, more preferably 15-80% by weight, and most preferably 15-50% by weight of the surfactant system.

[0020] 4. The use according to any one of the preceding items, wherein the hand-washing detergent is a fluid detergent composition, preferably an aqueous detergent composition.

[0021] 5. The use according to any one of the preceding items, wherein the hand washing detergent is a hand dishwashing composition or a liquid laundry detergent composition for hand washing.

[0022] 6. According to the use described in item 5, the rhamnose glycolipid is present in the composition in an amount of 1-20% by weight, preferably 1.25-15% by weight, more preferably 1.5-12.5% ​​by weight, and most preferably 2-10% by weight.

[0023] 7. The use according to any one of the foregoing items, wherein the rhamnose lipolipin comprises at least 50% by weight of dirhamnose lipolipin, more preferably at least 60% by weight of dirhamnose lipolipin, even more preferably 70% by weight of dirhamnose lipolipin, and most preferably at least 80% by weight of dirhamnose lipolipin.

[0024] 8. The use according to any one of the foregoing items, wherein the rhamnose glycolipid is of formula Rha2C 8-12 C 8-12 The two rhamnolipids. Detailed Implementation

[0025] Hand wash Handwashing detergents are detergents that involve consumers washing a substrate with their hands. Preferred handwashing uses primarily include laundry (i.e., hand washing of clothes) and handwashing (HDW) (i.e., hand washing of dishes, etc.). Handwashing detergents involve close contact between the detergent liquid and the hands during the washing process, whether it is during laundry or handwashing.

[0026] rhamnolipid Rhamnose glycolipids are a class of glycolipids. They are composed of rhamnose combined with β-hydroxy fatty acids. Rhamnose is a sugar. Fatty acids are ubiquitous in plants and animals.

[0027] Rhamnolipids are discussed in E. Deziel et al., Applied Microbiology and Biotechnology (2010) 86:1323-1336. Rhamnolipids are produced by Glycosurf, AGAE Technologies, and Urumqi UniteBio-Technology Co., Ltd. Rhamnolipids can be produced by strains of *Pseudomonas aeruginosa*. They can also be produced by recombinant cells of *Pseudomonas aeruginosa*, wherein the recombinant cells, compared to the wild-type cells, contain increased activity of at least one of enzymes a / P hydrolase, rhamnosyltransferase I, or rhamnosyltransferase II.

[0028] Rhamnose lipids are divided into two main categories: monorhamnose lipids and dirhamnose lipids.

[0029] Monorhamnolipids possess a single rhamnosine ring. A typical monorhamnolipid produced by *Pseudomonas aeruginosa* is L-rhamnosyl-β-hydroxydecyl-β-hydroxydecanoate (RhaC).10 C 10 It can be called Rha-C. 10 -C 10 It has the formula C 26 H 48 O9. Monorhamnolipids have a single rhamnosyl sugar ring.

[0030] The IUPAC name is 3-[3-[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylepoxyethylene-2-yl]oxydecanoyloxy]decanoic acid.

[0031] Dirhamnolipids have two rhamnosyl sugar rings. A typical dirhamnolipid is L-rhamnosyl-L-rhamnosyl-β-hydroxydecyl-β-hydroxydecanoate (Rha2C). 10 C 10 It can be called Rha-Rha-C- 10 -C- 10 , having formula C 32 H 58 O 13 .

[0032] The IUPAC name is 3-[3-[4,5-dihydroxy-6-methyl-3-(3,4,5-trihydroxy-6-methylepoxyethylene-2-yl)oxoepoxyethylene-2-yl]oxodecanoyloxy]decanoic acid.

[0033] In practice, depending on the carbon source and bacterial strain, a wide variety of other minor components with different combinations of alkyl chain lengths exist alongside the more common rhamnolipides described above. The ratio of monorhamnolipides to dirhamnolipides can be controlled by the production method. Some bacteria produce only monorhamnolipides (see US5767090: Example 1), and some enzymes can convert monorhamnolipides to dirhamnolipides.

[0034] In various published documents, monorhamnolipids are represented by the symbol Rha-, which can be abbreviated as Rh or RL2. Similarly, dirhamnolipids are represented by the symbols Rha-Rha or Rh-Rh- or RL1. For historical reasons, "rhamnolipid 2" refers to monorhamnolipids, and "rhamnolipid 1" refers to dirhamnolipids. This has led to some ambiguity in the usage of "RL1" and "RL2" in the literature.

[0035] In this patent specification, we use the terms monorhamnolipid and dirhamnolipid to avoid this potential confusion. However, if the abbreviations are used, R1 is monorhamnolipid and R2 is dirhamnolipid. For more information on the confusion of terminology in the prior art, please see the description of US 4814272.

[0036] The following rhamnolipids produced by the following bacteria have been detected: (C 12:1C 14:1 (This indicates a fatty acyl chain with a double bond).

[0037] Rhamnolipids (monorhamnetin) produced by Pseudomonas aeruginosa: Rha-C8''C 10 Rha-C 10 -C8、Rha-C 10 -C 10 Rha-C 10 -C 12 Rha-C 10 -C 12:1 Rha-C 12 -C 10 Rha-C 12:1 -C 10 Rhamnolipids (dirhamnolipids) produced by Pseudomonas aeruginosa: Rha-Rha-C8-C 10 Rha-Rha-C8-C 12:1 Rha-Rha-C 10 -C8、Rha-Rha-C 10 -C 10 Rha-Rha-C 10 -C 12:1 Rha-Rha-C 10 -C 12 Rha-Rha-C 12 -C 10 Rha-Rha-C 12:1 -C 12 Rha-Rha-C 10 -C 14:1 Rhamnolipids produced by *Pseudomonas aeruginosa* (not identified as mono- or di-rhamnolipids): C8-C8, C8-C 10 C 10 -C8、C8''C 12:1 C 12:1 -C8、C 10 -C 10 C 12 -C 10 C 12:1 -C 10 C 12 -C 12 C 12:1 -C 12 C 14 -C 10 C 14:1 -C 10C 14 -C 14 .

[0038] Rhamnolipids produced by *Pseudomonas aeruginosa* (monorhamnolipids only): Rha-C 10 -C8、Rha-C 10 -C 10 Rha-C 12 -C 10 Rha-C 12:1 -C 10 Rha-C 12 -C 12 Rha-C 12:1 -C 12 Rha-C 14 -C 10 Rha-C 14:1 -C 10 Rhamnolipids produced by Burkholderia melioides (dirhamnolipids only): Rha-Rha-C 14 -C 14 .

[0039] Burkholdera (Pseudomonas) plantarii The rhamnolipids produced (di-rhamnolipids only): Rha-Rha-C 14 -C 14 .

[0040] The American Type Culture Collection (ATCC) has over 100 strains of *Pseudomonas aeruginosa* documented. Several other strains are available only to manufacturers of commercial rhamnolipin. Furthermore, various research institutions worldwide have isolated potentially thousands of strains. Some work has progressed to grouping them. Each strain possesses distinct characteristics, including the amount of rhamnolipin produced, the type of rhamnolipin produced, its metabolism, and its growth conditions. Only a low percentage of strains have been extensively studied.

[0041] Through evaluation and screening, strains of *Pseudomonas aeruginosa* can be isolated to produce rhamnolipids at higher concentrations and more efficiently. Strains that produce fewer byproducts and metabolize different raw materials or contaminants can also be selected. This production is greatly influenced by the environment in which the bacteria grow.

[0042] A typical dirhamnolipid is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (formula C10) 32 H 58 O 13 Rha2C 10 C 10 ).

[0043] In practice, depending on the carbon source and bacterial strain, a wide variety of other minor components with different combinations of alkyl chain lengths exist alongside the more common rhamnolipides described above. The ratio of monorhamnolipides to dirhamnolipides can be controlled by the production method. Some bacteria produce only monorhamnolipides (see US5767090: Example 1), and some enzymes can convert monorhamnolipides to dirhamnolipides.

[0044] Preferably, rhamnolipin is present in the composition at a content of 1-20% by weight, more preferably 1.25-15% by weight, more preferably 1.5-12.5% ​​by weight, and most preferably 2-10% by weight.

[0045] Rhamnose lipids are preferably selected from: - Rhamnolipids (monorhamnetin) produced by Pseudomonas aeruginosa: Rha-C8-C 10 Rha-C 10 -C8、Rha-C 10 -C 10 Rha-C 10 -C 12 Rha-C 10 -C 12:1 Rha-C 12 -C 10 Rha-C 12:1 -C 10 - Rhamnolipids produced by *Pseudomonas aeruginosa* (monorhamnetin only): Rha-C 10 -C8、Rha-C 10 -C 10 Rha-C 12 -C 10 Rha-C 12:1 -C 10 Rha-C 12 -C 12 Rha-C 12:1 -C 12 Rha-C 14 -C 10 Rha-C 14:1 -C 10 - By introducing the rhlA and rhlB genes from *Pseudomonas aeruginosa*, monorhamnolipids produced by *Pseudomonas aeruginosa* can also be obtained [Cha et al., Bioresour Technol. 2008. 99(7):2192-9] - Rhamnolipids (dirhamnolipids) produced by Pseudomonas aeruginosa: Rha-Rha-C8-C 10 Rha-Rha-C8-C 12:1 Rha-Rha-C 10 -C8、Rha-Rha-C 10 -C 10 Rha-Rha-C 10 -C 12:1 Rha-Rha-C 10 -C 12 Rha-Rha-C 12 -C 10 Rha-Rha-C 12:1 -C 12 Rha-Rha-C 10 -C 14:1 - Rhamnolipids produced by Burkholderia melioides (dirhamnolipids only): Rha-Rha-C 14 -C 14 .

[0046] - Burkholdera (Pseudomonas) plantarii The rhamnolipids produced (di-rhamnolipids only): Rha-Rha-C 14 -C 14 .

[0047] - Rhamnolipids produced by Pseudomonas aeruginosa were initially not identified as monorhamnolipids or dirhamnolipids: C8-C8, C8-C 10 C 10 -C8、C8-C 12:1 C 12:1 -C8、C 10 -C 10 C 12 -C 10 C 12:1 -C 10 C 12 -C 12 C 12:1 -C 12 C 14 -C 10 C 14:1 -C 10 C 14 -C 14 .

[0048] Rhamnose lipolipide is preferably L-rhamnosyl-β-hydroxydecyl-β-hydroxydecanoate (formula C) 26 H 48 O9's RhaC 10 C10 ).

[0049] Preferably, the rhamnose glycolipid contains at least 50% by weight of dirhamnose glycolipid, more preferably at least 60% by weight of dirhamnose glycolipid, even more preferably 70% by weight of dirhamnose glycolipid, and most preferably at least 80% by weight of dirhamnose glycolipid.

[0050] Preferably, the rhamnose glycolipid is of formula Rha2C. 8-12 C 8-12 The dirhamnolipid. The preferred alkyl chain length is C8-C9. 12 Alkyl chains can be saturated or unsaturated.

[0051] The optimal dirhamnolipid is Rha2C. 8-12 C 8-12 An example of a rhamnolipin, referred to herein as rhamnolipin R2, is available from Evonik.

[0052] Hand wash detergent composition Handwash detergent compositions are cleaning compositions that can be used to clean one or more substrates.

[0053] The composition is preferably a fluid detergent composition, and more preferably an aqueous detergent composition.

[0054] Preferably, the handwashing detergent is a manual dishwashing composition or a liquid laundry detergent composition for handwashing.

[0055] Preferably, when the liquid laundry detergent is dissolved in demineralized water at 4 g / L, the pH of the hand-washing composition is 4-8, more preferably 4.5-7.5.

[0056] Preferably, when the liquid laundry detergent is dissolved in demineralized water at 4 g / L, the pH of the liquid laundry detergent composition is 6-11, more preferably 7-9.

[0057] Second anionic surfactant In the handwashing composition of the second aspect of the invention, the composition comprises a second different anionic surfactant.

[0058] The composition contains 1 to 20% by weight, preferably 1.5 to 17.5% by weight, more preferably 2 to 15% by weight, and most preferably 5 to 15% by weight of a second different anionic surfactant.

[0059] In the handwashing composition of the second aspect of the present invention, rhamnolipid is preferably present in the surfactant system at an amount of 10-90% by weight of the surfactant system, more preferably 15-80% by weight, and most preferably 15-50% by weight.

[0060] Preferably, in relation to the use of the first aspect of the invention, the composition comprises a second different anionic surfactant.

[0061] When using a second different anionic surfactant, rhamnolipid is preferably present in the surfactant system at a content of 10-90% by weight of the surfactant system, more preferably 15-80% by weight, and most preferably 15-50% by weight.

[0062] In all aspects of the invention, preferably, the second anionic surfactant is selected from C 10 To C 20 Linear alkylbenzene sulfonates, C 10 To C 20 Alkyl sulfates, C 10 To C 20 Alkyl ether sulfates and mixtures thereof. More preferably, the second different anionic surfactant is a mixture of the aforementioned anionic surfactants. More preferably, the second different anionic surfactant (b) is C 10 To C 20 linear alkylbenzene sulfonates and C 10 To C 20 A mixture of alkyl ether sulfates. Most preferably, C 10 To C 20 linear alkylbenzene sulfonates and C 10 To C 20 The mixture of alkyl ether sulfates is a mixture in a ratio of 10:90 to 90:10, preferably 20:80 to 80:20, and more preferably 30:70 to 70:30.

[0063] Additional surfactants Additional surfactants may be present in the composition.

[0064] Preferably, the cleaning composition contains 0-20% by weight, more preferably 0-10% by weight, of an additional surfactant.

[0065] These are preferably selected from other anionic, nonionic, and amphoteric surfactants.

[0066] Typically, the nonionic and anionic surfactants used in surfactant systems can be selected from the surfactants described in "Surface Active Agents," Volume 1, Schwartz & Perry, Interscience 1949; Volume 2, Schwartz, Perry & Berch, Interscience 1958; the current edition of "McCutcheon's Emulsifiers and Detergents" published by Manufacturing Confectioners Company; or "Tenside Taschenbuch," H. Stache, 2nd edition, Carl Hauser Verlag, 1981. Preferably, the surfactant used is saturated.

[0067] Preferred nonionic detergent compounds that can be used include compounds having hydrophobic groups and reactive hydrogen atoms, such as aliphatic alcohols, acids, and amides, as reaction products with alkyl oxides, particularly ethylene oxide alone or with propylene oxide. Specific nonionic detergent compounds are condensation products of aliphatic straight-chain or branched primary or secondary alcohols with ethylene oxide, typically ranging from 5 to 40 EO, preferably from 7 to 9 EO.

[0068] Preferred anionic detergent compounds that can be used are typically water-soluble alkali metal salts of organic sulfuric acid and sulfonic acid containing an alkyl group of about 8 to about 22 carbon atoms, the term alkyl being used for the alkyl moiety including a higher acyl group.

[0069] Suitable examples of synthetic anionic detergent compounds are sodium alkyl sulfate and potassium alkyl sulfate, especially those obtained by sulfation of alcohols, for example, derived from tallow or coconut oil, with alkyl C... 10 To C 20 Sodium and potassium benzenesulfonate, especially straight-chain secondary alkyl C 10 To C 15 Sodium benzenesulfonate; and sodium alkyl glycerol ether sulfate, particularly those ethers derived from higher alcohols derived from tallow or coconut oil and synthetic alcohols derived from petroleum. Preferred anionic detergent compounds are C... 11 -C 15 Sodium alkylbenzenesulfonate and C 12 -C 14 Sodium alkyl sulfate. Also applicable are surfactants such as those exhibiting resistance to salting out as described in EP-A-328 177 (Unilever), alkyl polyglycoside surfactants as described in EP-A-070 074, and alkyl monosaccharides.

[0070] Preferred surfactant systems are mixtures of anionic and nonionic detergent active materials, particularly the groups and examples of anionic and nonionic surfactants specified in EP-A-346 995 (Unilever). Especially preferred are surfactant systems consisting of the aforementioned anionic surfactant and C... 12 -C 16 A mixture of primary alcohols 3-7 EO ethoxylates.

[0071] Preferred amphoteric surfactants include cocamidopropyl betaine. The preferred content of the amphoteric surfactant is 0.1-5% by weight, more preferably 0.5-4% by weight.

[0072] Other ingredients Depending on whether the hand wash composition is a manual dishwashing composition or a liquid laundry detergent composition for hand washing, the hand wash composition may contain any of these further preferred ingredients.

[0073] Builders or complexing agents Detergent additives can be particularly useful in liquid laundry detergent compositions intended for hand washing.

[0074] The detergent building block material can be selected from 1) calcium chelating agent material, 2) deposition material, 3) calcium ion exchange material, and 4) mixtures thereof.

[0075] Examples of calcium chelating agent builder materials include alkali metal polyphosphates, such as sodium triphosphate, and organic chelating agents, such as ethylenediaminetetraacetic acid.

[0076] Examples of precipitating detergent materials include sodium orthophosphate and sodium carbonate.

[0077] Examples of calcium ion exchange detergent building materials include various types of water-insoluble crystalline or amorphous aluminosilicates, among which zeolites are the most well-known representatives, such as zeolite A, zeolite B (also known as zeolite P), zeolite C, zeolite X, zeolite Y, and type P zeolite described in EP-A-0,384,070.

[0078] The composition may also contain 0-65% by weight of a builder or complexing agent, such as ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, alkyl or alkenyl succinic acid, hypozinotriacetic acid, or other builder mentioned below. Many builder agents are also bleach stabilizers due to their ability to complex metal ions.

[0079] Zeolites and carbonates (including bicarbonates and sesquicarbonates) are preferred detergent builders.

[0080] The composition may contain crystalline aluminosilicates as a detergent builder, preferably alkali metal aluminosilicates, and more preferably sodium aluminosilicates. This is typically present in an amount of less than 15% by weight. Aluminosilicates are materials having the following general formula: 0.8-1.5 M2O·Al2O3·0.8-6 SiO2 Where M is a monovalent cation, preferably sodium. These materials contain some bound water and are required to have a calcium ion exchange capacity of at least 50 mg CaO / g. In the above formula, the preferred sodium aluminosilicate contains 1.5-3.5 SiO2 units. They can be readily prepared by a reaction between sodium silicate and sodium aluminate, as described in detail in the literature. The ratio of surfactant to aluminosilicate (in the presence of the surfactant) is preferably greater than 5:2, more preferably greater than 3:1.

[0081] Alternatively, phosphate-based builders may be used in addition to aluminosilicate builders. In this art, the term "phosphate" includes diphosphates, triphosphates, and phosphonates. Other forms of builders include silicates, such as soluble silicates, metasilicates, and layered silicates (e.g., SKS-6 from Hoechst).

[0082] When used in laundry compositions, preferably, the laundry detergent formulation is a non-phosphate-based laundry detergent formulation, i.e., containing less than 1% by weight of phosphate. Preferably, if a detergent builder is included, the laundry detergent formulation is a carbonate-based detergent builder.

[0083] fluorescent agent These materials can be particularly useful in liquid laundry detergent compositions for hand washing.

[0084] The composition preferably contains a fluorescent agent (optical brightener).

[0085] The total amount of one or more fluorescent agents used in the composition is typically 0.005-2% by weight, more preferably 0.01-0.1% by weight. Preferred fluorescent agent classes are: stilbene biphenyl compounds, such as Tinopal (trademark) CBS-X; diamine stilbene disulfonic acid compounds, such as Tinopal DMS pure Xtra and Blankophor (trademark) HRH; and pyrazoline compounds, such as Blankophor SN. Preferred fluorescent agents are: sodium 2(4-styryl-3-sulfonylphenyl)-2H-naphthol[1,2-d]triazole, sodium 4,4'-bis{[(4-anilino-6-(N-methyl-N-2-hydroxyethyl)amino-1,3,5-triazin-2-yl)]amino}stilbene-2,2'-disulfonate, sodium 4,4'-bis{[(4-anilino-6-morpholino-1,3,5-triazin-2-yl)]amino}stilbene-2,2'-disulfonate, and sodium 4,4'-bis(2-sulfonyl)biphenyl.

[0086] Preferably, the aqueous solution used in the method contains a fluorescent agent. When the fluorescent agent is present in the aqueous solution used in the method, it is preferably in the range of 0.0001 g / L to 0.1 g / L, more preferably 0.001 g / L to 0.02 g / L.

[0087] dye The composition preferably contains a dye. The dye is discussed in K. Hunger (ed.). Industrial Dyes: Chemistry, Properties, Applications (Weinheim: Wiley-VCH 2003). Organic dyes are listed in color index (Society of Dyers and Colourists and the American Association of Textile Chemists and Colorists).

[0088] Preferred dye chromophores are azo, azazine, anthraquinone, phthalocyanine, and triphenylmethane.

[0089] Azo, anthraquinone, phthalocyanine, and triphenylmethane dyes are preferably net anions with or without charge. Azazine dyes are preferably net anions or cationic charges.

[0090] The preferred non-toning dyes are selected from blue dyes, with anthraquinone dyes and triphenylmethane dyes containing sulfonate groups being most preferred. Preferred compounds are Acid Blue 80, Acid Blue 1, Acid Blue 3; Acid Blue 5, Acid Blue 7, Acid Blue 9, Acid Blue 11, Acid Blue 13, Acid Blue 15, Acid Blue 17, Acid Blue 24, Acid Blue 34, Acid Blue 38, Acid Blue 75, Acid Blue 83, Acid Blue 91, Acid Blue 97, Acid Blue 93, Acid Blue 93:1, Acid Blue 97, Acid Blue 100, Acid Blue 103, Acid Blue 104, Acid Blue 108, Acid Blue 109, Acid Blue 110, and Acid Blue 213. When dissolved, the particles containing the non-toning dyes provide an attractive color to the washing liquid.

[0091] Blue or purple tinting dyes are most preferred. The tinting dye is deposited onto the fabric during the washing or rinsing step of the washing process, providing the fabric with a visible hue. In this respect, the dye imparts blue or purple to white garments with a hue angle of 240 to 345, more preferably 260 to 320, and most preferably 270 to 300. The white garments used in this test were bleached, non-mercerized woven cotton sheets.

[0092] The tinting dyes are WO2005 / 003274, WO2006 / 032327 (Unilever), WO2006 / 032397 (Unilever), WO2006 / 045275 (Unilever), WO2006 / 027086 (Unilever), WO2008 / 017570 (Unilever), WO2008 / 141880 (Unilever), WO2009 / 132870 (Unilever), WO2 Discussed in 009 / 141173 (Unilever), WO2010 / 099997 (Unilever), WO2010 / 102861 (Unilever), WO2010 / 148624 (Unilever), WO2008 / 087497 (P&G), WO2011 / 011799 (P&G), WO2012 / 054820 (P&G), WO2013 / 142495 (P&G) and WO2013 / 151970 (P&G).

[0093] A mixture of color dyes can be used.

[0094] The chromophores of the tinting dyes are preferably selected from monoazo, diazo, anthraquinone, and azazine.

[0095] Monoazo dyes preferably contain heterocycles, and most preferably thiophene dyes. Monoazo dyes are preferably alkoxylated, and preferably uncharged or anionic at pH 7. Alkoxylated thiophene dyes are discussed in WO 2013 / 142495 and WO 2008 / 087497.

[0096] The preferred tinting dyes are selected from Direct Violet 9, Direct Violet 99, Direct Violet 35, Solvent Violet 13, and Disperse Violet 28, and have the following structures. , , , , .

[0097] spices Preferably, the composition comprises a fragrance. The fragrance is preferably present in the range of 0.001 to 3% by weight, most preferably 0.1 to 1% by weight. Examples of many suitable fragrances are provided in the CTFA (Cosmetic, Toiletry and Fragrance Association) 1992 International Buyers Guide published by CFTA Publications and the OPD 1993 Chemicals Buyers Directory, 1980 edition published by Schnell Publishing Co.

[0098] It is common for formulations to contain multiple flavoring components. In the compositions of the present invention, it is envisioned that there are four or more, preferably five or more, more preferably six or more, or even seven or more different flavoring components.

[0099] In the fragrance blend, preferably 15 to 25 percent by weight is a top note. Top notes are defined by Poucher (Journal of the Society of Cosmetic Chemists 6(2):80

[1955] ). Preferred top notes are selected from citrus oil, linalool, linalyl acetate, lavender, dihydromyrcenol, rose ether, and cis-3-hexanol.

[0100] Preferably, the laundry treatment composition does not contain peroxide bleach, such as sodium percarbonate, sodium perborate, and peracid.

[0101] polymer The composition may contain one or more other polymers. Examples are carboxymethyl cellulose, poly(ethylene glycol), poly(vinyl alcohol), polycarboxylic acid esters such as polyacrylates, maleic acid / acrylic acid copolymers, and lauryl methacrylate / acrylic acid copolymers. Polymers that prevent dye deposition, such as poly(vinylpyrrolidone), poly(vinylpyridine-N-oxide), and poly(vinylimidazole), may be present in the formulation.

[0102] It may contain thickening polymers such as anionic acrylic polymers, examples of which include Acusol 820.

[0103] enzymes These materials can be particularly useful in liquid laundry detergent compositions for hand washing.

[0104] When implementing the method of the present invention, one or more enzymes are preferably present in the cleaning composition of the present invention.

[0105] Preferably, the content of each enzyme in the laundry composition of the present invention is 0.0001%-0.1% protein by weight.

[0106] Enzymes specifically envisioned include proteases, α-amylases, cellulases, lipases, peroxidases / oxidases, pectic acid lyases, and mannanases, or mixtures thereof.

[0107] Suitable lipases include those of bacterial or fungal origin. This includes chemically modified or protein-engineered mutants. Examples of useful lipases include those from *Pythium spp.* (…). Humicola (Synonyms: thermophilic fungi) Thermomyces (e.g., from EP 258 068 and EP 305 216) H. lanuginosa ( T. lanuginosus (or as described in WO 96 / 13580) H. insolens ; Pseudomonas lipases, such as those from Alcaligenes ( P. alcaligenes ) or Pseudomonas alcaligenes ( P. pseudoalcaligenes (EP 218 272), Pseudomonas cepacia ( P. cepacia (EP 331 376), Pseudomonas stearothermia ( P. stutzeri (GB 1,372,034), Fluorescent Pseudomonas ( P. fluorescens ), Pseudomonas strains SD 705 (WO 95 / 06720 and WO 96 / 27002), P. wisconsinensis (WO 96 / 12012); Bacillus lipases, such as those from Bacillus subtilis ( B. subtilis (Dartois et al. (1993), Biochemica et Biophysica Acta, 1131, 253-360), Bacillus stearothermophilus ( B. stearothermophilus (JP 64 / 744992) or Bacillus pumilus ( B. pumilus (WO 91 / 16422).

[0108] Other examples are lipase variants, such as those described in WO 92 / 05249, WO 94 / 01541, EP 407 225, EP 260105, WO 95 / 35381, WO 96 / 00292, WO 95 / 30744, WO 94 / 25578, WO 95 / 14783, WO 95 / 22615, WO 97 / 04079, WO 97 / 07202, and WO 00 / 60063.

[0109] Preferred commercially available lipases include Lipolase™ and Lipolase Ultra™, Lipex™ and Lipoclean. TM(Novozymes A / S).

[0110] The method of the present invention can be carried out in the presence of phospholipases classified as EC 3.1.1.4 and / or EC 3.1.1.32. As used herein, the term phospholipase refers to an enzyme that is active in phospholipids.

[0111] Phospholipids, such as lecithin or phosphatidylcholine, consist of glycerol esterified with two fatty acids at the external (sn-1) and middle (sn-2) positions, and phosphorylated at the third position; the phosphate group can, in turn, be esterified to an amino alcohol. Phospholipases are enzymes involved in the hydrolysis of phospholipids. Several types of phospholipase activity can be distinguished, including phospholipases A1 and A2, which hydrolyze a fatty acyl group (at the sn-1 and sn-2 positions, respectively) to form lysophospholipids; and lysophospholipases (or phospholipase B), which can hydrolyze the remaining fatty acyl group in lysophospholipids.

[0112] Phospholipase C and phospholipase D (phosphodiesterase) release diacylglycerol or phosphatidic acid, respectively.

[0113] Enzymes and photobleaching agents can exhibit some interactions, and selection should be made to ensure that such interactions are not negative. Some negative interactions can be avoided by encapsulating one or more enzymes or photobleaching agents within the product and / or by using other barriers.

[0114] Suitable proteases include those of animal, plant, or microbial origin. Microbial origin is preferred. This includes chemically modified or protein-engineered mutants. The protease can be a serine protease or a metalloproteinase, preferably an alkaline microbial protease or a trypsin-like protease. Preferred commercially available proteases include Alcalase™, Savinase™, Primase™, Duralase™, Dyrazym™, Esperase™, Everlase™, Polarzyme™, and Kannase™ (Novozymes A / S), Maxatase™, Maxacal™, Maxapem™, Properase™, Purafect™, Purafect OxP™, FN2™, and FN3™ (Genencor International Inc.).

[0115] The method of the present invention can be carried out in the presence of a keratinase classified as EC 3.1.1.74. The keratinase used according to the present invention can be from any source.

[0116] Preferably, the keratinase has a microbial origin, particularly bacteria, fungi, or yeast.

[0117] Suitable amylases (α and / or β) include those of bacterial or fungal origin. This includes chemically modified or protein-engineered mutants. Amylases include, for example, α-amylases derived from Bacillus strains, such as specific strains of Bacillus licheniformis as described more in GB 1,296,839, or Bacillus strains disclosed in WO 95 / 026397 or WO 00 / 060060. Commercially available amylases are Duramyl™, Termamyl™, Termamyl Ultra™, Natalase™, Stainzyme™, Fungamyl™, and BAN™ (Novozymes A / S), Rapidase™, and Purastar™ (from Genencor International Inc.).

[0118] Suitable cellulases include those of bacterial or fungal origin. This includes chemically modified or protein-engineered mutants. Suitable cellulases include those derived from the following genera: *Bacillus*, *Pseudomonas*, *Pyrophyllus*, *Fusarium*, *Clostridium*, *Clostridium*, *Clostridium*, fungal cellulases, such as those produced by *Pyrophyllus*, *Clostridium thaliana*, *Thermophilus*, and *Fusarium* as disclosed in US 4,435,307, US 5,648,263, US 5,691,178, US 5,776,757, WO 89 / 09259, WO 96 / 029397, and WO 98 / 012307. Commercially available cellulases include Celluzyme™, Carezyme™, Cellluclean™, Endolase™, Renozyme™ (Novozymes A / S), Clazinase™, Puradax HA™ (Genencor International Inc.), and KAC-500(B)™ (Kao Corporation).

[0119] Suitable peroxidases / oxidases include those of plant, bacterial, or fungal origin. This includes chemically modified or protein-engineered mutants. Examples of useful peroxidases include peroxidases from the genus *Coprinus*, such as those from *Coprinus spp.*, and their variants, as described in WO 93 / 24618, WO 95 / 10602, and WO 98 / 15257. Commercially available peroxidases include Guardzyme™ and Novozym™ 51004 (Novozymes A / S).

[0120] Other applicable enzymes are discussed in WO2009 / 087524, WO2009 / 090576, WO2009 / 107091, WO2009 / 111258 and WO2009 / 148983.

[0121] Enzyme stabilizers Any enzyme present in the composition may be stabilized using conventional stabilizers, such as polyols, like propylene glycol or glycerol; sugars or sugar alcohols; lactic acid; boric acid or boric acid derivatives, such as aromatic borate esters, or phenyl boric acid derivatives, such as 4-formylphenylboronic acid, and the composition may be formulated as described, for example, in WO 92 / 19709 and WO 92 / 19708.

[0122] When the alkyl group is long enough to form a branched or cyclic chain, the alkyl group includes branched, cyclic, and straight-chain alkyl chains. The alkyl group is preferably straight-chain or branched, with straight-chain being the most preferred.

[0123] Unless otherwise stated, the indefinite article “a / an” and its corresponding definite article “the” as used herein refer to at least one / a kind, one / a kind, or more / a kind.

[0124] The invention will be further described through the following non-limiting embodiments.

[0125] Example The purpose of the expert panel evaluation is to identify and measure differences in specific sensory properties between the product and the control formulation. This is done through blind testing of the product. The study ensures that all panel members see and evaluate all prototype and control products in a randomized order. The panel members are trained members: these are members screened for sensory acuity and trained in analytical techniques, and there are 12-15 panel members in total.

[0126] The group members worked using a 0-10 scale, with two positioning points: 1 for not very mild and 9 for very mild.

[0127] After the washing process, the group members waited 10 minutes while their hands dripped dry. They then assessed the smoothness of their hands.

[0128] For the mildness attribute, the following sensory attributes are measured: The mildness of hands after drying refers to the creamy / layered impression felt on hands after drying; it is assessed by sliding fingers between the surface of the hand and feeling with the thumb on the other fingers, and is again measured on a rating scale between not mild at all and very mild.

[0129] The formulation used – control

[0130] The control formulations all had an acceptable viscosity of 1180 to 1610 centipoise (cP).

[0131] Viscosity was measured at 25°C using an Anton Paar ASC rheometer – settings were used with Bob and the result was reported at 23 s. -1 Viscosity measured at shear rate The formulation used—according to the present invention

[0132] Rhamnose lipolipid R2 is a dirhamnose lipolipid supplied by Evonik.

[0133] The formulations according to the invention all have an acceptable viscosity between 1350 and 1520 centipoise (cP).

[0134] Viscosity was measured at 25°C using an Anton Paar ASC rheometer – settings were used with Bob and the result was reported at 23 s. -1 Viscosity measured at shear rate.

[0135] Results table of long-lasting, mild properties 1. Long-term mildness compared to controls 1 to 3

[0136] Formulation 1 showed a statistically significant improvement in this property compared to controls 1 to 3 (Tukey's HSD test, 95% confidence interval).

[0137] 2. Long-term mildness compared to controls 1 to 3

[0138] Formulation 2 showed a statistically significant improvement in this property compared to controls 1 to 3 (Tukey's HSD test, 95% confidence interval).

[0139] 3. Long-term mildness compared to controls 1 to 3

[0140] Formulation 3 showed a statistically significant improvement in this property compared to controls 1 to 3 (Tukey's HSD test, 95% confidence interval).

[0141] 4. Long-term mildness compared to controls 1 to 3

[0142] Formulation 4 showed a statistically significant improvement in this property compared to controls 1 to 3 (Tukey's HSD test, 95% confidence interval).

[0143] 5. Long-term mildness compared to controls 1 to 3

[0144] Formulation 5 showed a statistically significant improvement in this property compared to controls 1 to 3 (Tukey's HSD test, 95% confidence interval).

[0145] This data shows that the use of rhamnolipids in surfactant systems for handwashing detergents imparts a gentle feeling to consumers' hands. This gentle feeling is particularly noticeable after the handwashing process, when the hands are subsequently dried, and the gentle sensation persists.

Claims

1. The use of rhamnolipin in surfactant systems for hand washing detergents to impart a mild feeling to the hands of consumers, wherein the rhamnolipin is present in the surfactant system at a content of 15-50% by weight of the surfactant system, wherein the use relates to the close contact of detergent liquid with the hands during washing, whether during laundry or hand washing dishes.

2. The use according to claim 1, wherein the mild sensation on the hands persists after the hand washing process is completed and the hands have subsequently dried.

3. The use according to any one of the preceding claims, wherein the handwashing detergent is a fluid detergent composition, preferably an aqueous detergent composition.

4. The use according to any one of the preceding claims, wherein the hand washing detergent is a hand dishwashing composition or a liquid laundry detergent composition for hand washing.

5. The use according to claim 4, wherein the rhamnose glycolipid is present in the composition in an amount of 1-20% by weight, preferably 1.25-15% by weight, more preferably 1.5-12.5% ​​by weight, and most preferably 2-10% by weight.

6. The use according to any one of the preceding claims, wherein the rhamnose lipolipin comprises at least 50% by weight of dirhamnose lipolipin, more preferably at least 60% by weight of dirhamnose lipolipin, even more preferably 70% by weight of dirhamnose lipolipin, and most preferably at least 80% by weight of dirhamnose lipolipin.

7. The use according to any one of the preceding claims, wherein the rhamnose glycolipid is of formula Rha2C 8-12 C 8-12 The two rhamnolipids.

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