Lactobacillus brevis, lactobacillus brevis agent, composite biological deodorant, and preparation method and application thereof

Abstract

This invention provides *Lactobacillus brevis*, *Lactobacillus brevis* inoculum, a compound biological deodorant, its preparation method, and its application, relating to the field of microbiology. Specifically, the *Lactobacillus brevis* is *Lactobacillus brevis* (…). Lactobacillus brevis Lb-sj 001, with accession number CGMCC No. 36908. This invention provides a bacterial agent containing this *Lactobacillus brevis*, as well as the application of the strain and the bacterial agent in the preparation of deodorants, antibacterial agents, or acaricides. It also provides a composite biological deodorant containing *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj. *Lactobacillus brevis* Lb-sj 001 is a multifunctional deodorizing strain that, while exhibiting excellent deodorizing effects, also inhibits bacteria and kills mites, making it particularly suitable for the development and application of pet deodorants. This invention overcomes the shortcomings of current deodorizing strains, which typically have relatively limited functionality and practicality.

Description

Technical Field

[0001] This invention relates to the field of microbiology, specifically to a Lactobacillus brevis, a Lactobacillus brevis inoculum, a compound biological deodorant, and their preparation methods and applications. Background Technology

[0002] As more and more pets become family companions, the odors they produce (such as breath, skin, and excrement odors) not only affect the quality of life but may also contain harmful gases such as hydrogen sulfide and ammonia, which can affect human health. Currently, odor has become one of the core pain points for pet-owning families, and pet deodorizers are the core means to solve this problem.

[0003] There are many types of pet deodorizers on the market, but they still have problems such as poor effectiveness and insufficient safety of ingredients. Physical (such as activated carbon) and chemical deodorizers suffer from problems such as rapid adsorption saturation and low safety, making them unsuitable for long-term use. Furthermore, chemical deodorizers (such as ammonia and bleach) often employ a "masking" approach, covering odors with fragrances. While they can quickly eliminate odors, they cannot eradicate them completely and may even worsen irritation, damaging a pet's sense of smell or triggering allergies. Plant-derived deodorizers use natural ingredients to neutralize odors and inhibit bacteria, are non-toxic and harmless, and suitable for pet licking environments; however, their deodorizing efficiency and long-lasting effect are insufficient. Bio-enzyme deodorizers use complex bio-enzymes to decompose odor molecules, achieving "source deodorization," and are currently used to replace traditional chemical neutralization methods. Although these deodorizers show some effectiveness in treating certain malodorous gases, their function is limited, and they have no antibacterial effect on pathogenic microorganisms in the pet environment. Microbial deodorizers are a new type of deodorizer developed based on the deodorizing action of microorganisms. They are highly safe and do not pose a health hazard to pets, and have been extensively studied; however, their function is usually relatively limited, and their practicality needs improvement.

[0004] Therefore, there is an urgent need in this field to obtain a multifunctional microbial and multi-effect biological deodorizer with deodorization, antibacterial, and mite-killing properties. Summary of the Invention

[0005] Therefore, the purpose of this invention is to provide a multifunctional microorganism and multi-effect biological deodorizer with deodorization, antibacterial, and mite-killing properties.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] In a first aspect, the present invention provides a *Lactobacillus brevis*, specifically *Lactobacillus brevis* (…). Lactobacillus brevis Lb-sj 001, with accession number CGMCC No. 36908.

[0008] The present invention provides Lactobacillus brevis ( Lactobacillus brevisLb-sj 001 was deposited on December 5, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36908.

[0009] Secondly, the present invention provides a Lactobacillus brevis preparation containing Lactobacillus brevis with accession number CGMCC No. 36908. Lactobacillus brevis )Lb-sj 001.

[0010] Thirdly, the present invention provides the use of the aforementioned Lactobacillus brevis or the aforementioned Lactobacillus brevis agent in the preparation of deodorants, antibacterial agents or acaricides.

[0011] Furthermore, the deodorizer is used to remove ammonia and / or hydrogen sulfide.

[0012] Furthermore, the antibacterial agent is used to inhibit the growth and reproduction of at least one of Escherichia coli, Staphylococcus aureus, and Salmonella.

[0013] Furthermore, the Salmonella mentioned is Salmonella pullorum.

[0014] Fourthly, the present invention provides a compound biological deodorant containing *Lactobacillus brevis* and *Lactobacillus plantarum*, wherein the *Lactobacillus brevis* is specifically *Lactobacillus brevis* (… Lactobacillus brevis Lb-sj 001, with accession number CGMCC No. 36908, specifically refers to *Lactobacillus plantarum* (Lactobacillus plantarum). Lactobacillus plantarum )LP-sj, with accession number CGMCC No. 9513.

[0015] Lactobacillus brevis ( Lactobacillus brevis Lb-sj 001 was deposited on December 5, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36908.

[0016] Lactobacillus plantarum ( Lactobacillus plantarum LP-sj was deposited on August 15, 2014, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 9513. This strain has been published in Chinese patent document CN104357348A, published on February 18, 2015.

[0017] Furthermore, the ratio of viable bacteria of the *Lactobacillus brevis* to *Lactobacillus plantarum* is 1-3:1-2.

[0018] Furthermore, the ratio of viable bacteria of the *Lactobacillus brevis* and the *Lactobacillus plantarum* is 1:1, 2:1, 3:1, or 1:2.

[0019] Furthermore, the total number of viable bacteria in the composite biological deodorizer is not less than 2 × 10⁻⁶. 8 cfu / mL.

[0020] Furthermore, the compound biological deodorizer contains Lactobacillus brevis fermentation broth and Lactobacillus plantarum fermentation broth.

[0021] Furthermore, based on the mass percentage of the composite biological deodorizer, the composite biological deodorizer also contains at least one of the following components: 5%~15% humectant, 0.5%~1.5% surfactant, 0.02%~0.06% chelating agent, and 0%~5% flavoring agent, with the balance being deionized water.

[0022] Furthermore, based on the mass percentage of the composite biological deodorizer, the composite biological deodorizer also contains the following components: 5%~15% humectant, 0.5%~1.5% surfactant, 0.02%~0.06% chelating agent, and 0%~5% flavoring agent, with the balance being deionized water.

[0023] Furthermore, based on the mass percentage of the composite biological deodorizer, the composite biological deodorizer also contains the following components: 5%~10% humectant, 0.8%~1.2% surfactant, 0.03%~0.05% chelating agent, and 0%~2% flavoring agent, with the balance being deionized water.

[0024] Furthermore, based on the mass percentage of the composite biological deodorizer, the composite biological deodorizer also contains the following components: 5% humectant, 1.0% surfactant, 0.05% chelating agent, and 1% flavoring agent, with the balance being deionized water.

[0025] Furthermore, the moisturizer is glycerin and / or propylene glycol.

[0026] Furthermore, the surfactant is poloxamer.

[0027] Furthermore, the chelating agent is ethylenediaminetetraacetic acid.

[0028] Fifthly, the present invention provides a method for preparing the aforementioned composite biological deodorizer, comprising the following steps: S1. Preparation of seed culture: The *Lactobacillus brevis* is inoculated into MRS medium and cultured statically to obtain *Lactobacillus brevis* seed culture; the *Lactobacillus plantarum* is inoculated into MRS medium and cultured statically to obtain *Lactobacillus plantarum* seed culture. S2. Preparation of fermentation broth: The Lactobacillus brevis seed culture is inoculated into Lactobacillus brevis fermentation medium and fermented to obtain Lactobacillus brevis fermentation broth; The Lactobacillus plantarum seed culture is inoculated into Lactobacillus plantarum fermentation medium and fermented to obtain Lactobacillus plantarum fermentation broth; S3. Preparation of compound biological deodorant: The fermentation broth of Lactobacillus brevis and the fermentation broth of Lactobacillus plantarum are mixed evenly without the addition of excipients or with at least one of the following excipients: humectant, surfactant, chelating agent and flavor modifier, and the pH value of the mixture is adjusted to acidic to obtain the compound biological deodorant.

[0029] Further, in step S1, the static culture conditions for the Lactobacillus brevis are: static culture at 35~37 ℃ for 18~24 h.

[0030] Further, in step S1, the static culture conditions for the Lactobacillus brevis are: static culture at 37 °C for 18 h.

[0031] Further, in step S1, the static culture conditions for the *Lactobacillus plantarum* are: static culture at 35-37 ℃ for 18-24 h.

[0032] Furthermore, in step S1, the static culture conditions for the *Lactobacillus plantarum* are: static culture at 37 °C for 18 h.

[0033] Further, in step S2, the fermentation culture conditions for the *Lactobacillus brevis* are as follows: aeration and stirring at 35-37 ℃ and 100-150 rpm for 18-24 h, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 1×10⁻⁶. 9 ~3×10 9 cfu / mL.

[0034] Further, in step S2, the fermentation culture conditions for the *Lactobacillus brevis* are as follows: cultured at 35 ℃ and 150 rpm with aeration and stirring for 24 h, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 2 × 10⁻⁶. 9 cfu / mL.

[0035] Further, in step S2, the fermentation culture conditions for *Lactobacillus plantarum* are as follows: culture at 35-37 ℃ and 100-150 rpm with aeration and stirring for 18-24 h, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 1×10⁻⁶. 10 ~2×10 10 cfu / mL.

[0036] Further, in step S2, the fermentation culture conditions for *Lactobacillus plantarum* are as follows: cultured at 37 ℃ and 150 rpm with aeration and stirring for 24 h, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 1.8 × 10⁻⁶.10 cfu / mL.

[0037] Further, in step S2, the inoculation amount of the Lactobacillus brevis seed liquid is 5% to 10% of the volume of the Lactobacillus brevis fermentation culture medium, preferably 5%.

[0038] Further, in step S2, the inoculation amount of the Lactobacillus plantarum seed liquid is 5% to 10% of the volume of the Lactobacillus plantarum fermentation culture medium, preferably 5%.

[0039] Furthermore, in step S2, the amount of the Lactobacillus brevis fermentation medium accounts for 50% to 80% of the total volume of the fermenter, preferably 60%.

[0040] Furthermore, in step S2, the amount of the Lactobacillus plantarum fermentation culture medium accounts for 50% to 80% of the total volume of the fermenter, preferably 60%.

[0041] Further, in step S2, the Lactobacillus brevis fermentation medium comprises the following components by mass percentage: lactose 5%–10%, peptone 1%–2%, yeast extract 4%–8%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, and the remainder is water; the pH value of the Lactobacillus brevis fermentation medium is 5.5–6.0.

[0042] Further, in step S2, the Lactobacillus brevis fermentation medium comprises the following components by mass percentage: 5% lactose, 1% peptone, 4% yeast extract, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, and the remainder is water; the pH value of the Lactobacillus brevis fermentation medium is 5.5.

[0043] Further, in step S2, the *Lactobacillus plantarum* fermentation medium comprises the following components by mass percentage: 5%–10% glucose, 0.5%–1% beef meal, 0.5%–1% peptone, 2%–5% yeast powder, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, and the remainder is water; the pH value of the *Lactobacillus plantarum* fermentation medium is 5.5–6.0.

[0044] Further, in step S2, the *Lactobacillus plantarum* fermentation medium comprises the following components by mass percentage: 5% glucose, 0.5% beef meal, 0.5% peptone, 2% yeast powder, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, and the remainder is water; the pH value of the *Lactobacillus plantarum* fermentation medium is 5.5.

[0045] Further, in step S3, the pH value is adjusted to 2.5~4.0.

[0046] Further, in step S3, the pH value is adjusted to 3.6~3.7.

[0047] Sixthly, the present invention provides the application of the composite biological deodorizer or the composite biological deodorizer obtained by the preparation method described above in deodorization, antibacterial or mite-killing.

[0048] Furthermore, the deodorization is the removal of ammonia and / or hydrogen sulfide.

[0049] Furthermore, the antibacterial effect is to inhibit the growth and reproduction of at least one of Escherichia coli, Staphylococcus aureus, and Salmonella.

[0050] Furthermore, the Salmonella mentioned is Salmonella pullorum.

[0051] Furthermore, the composite biological deodorizer is used as a deodorizer for pet supplies, pet feces, pet odor, textile products, kitchen waste, home environment, vehicle interior environment, farm environment, or public toilet environment.

[0052] The technical solution of this invention has the following advantages: 1. The short lactobacillus provided by this invention ( Lactobacillus brevis Lb-sj 001 possesses multiple functions including deodorization, antibacterial, and mite elimination. It achieves a 95.36% ammonia removal rate within 1 hour, 18.7% higher than commercially available Lactobacillus brevis strains; an 80.0% hydrogen sulfide removal rate within 4 hours, 29% higher than commercially available Lactobacillus brevis strains; an 85.00% inhibition rate against Escherichia coli, 19% higher than commercially available Lactobacillus brevis strains; an 81.00% inhibition rate against Staphylococcus aureus, 12% higher than commercially available Lactobacillus brevis strains; an 83.00% inhibition rate against Salmonella, 16% higher than commercially available Lactobacillus brevis strains; and a mite elimination rate of 69.67%, 21.5% higher than commercially available Lactobacillus brevis strains. As this Lactobacillus brevis strain Lb-sj 001 is a multi-functional deodorizing strain, it not only provides excellent deodorization but also inhibits bacteria and eliminates mites, making it particularly suitable for the development and application of pet deodorants.

[0053] 2. The compound biological deodorizer provided by this invention contains Lactobacillus brevis (… Lactobacillus brevis Lb-sj001 and Lactobacillus plantarum ( Lactobacillus plantarumLP-sj, a compound of two bacterial strains, exhibits excellent rapid and long-lasting deodorization effects, while also possessing significant antibacterial and anti-mite properties. This compound biological deodorizer has a light fermented fragrance and shows significant odor removal effects after spraying in pet environments. Ammonia removal rate tests show a removal rate of ≥99% after 1 hour and ≥90% after 24 hours, achieving highly efficient ammonia removal. Hydrogen sulfide removal rate tests show a removal rate of ≥80% after 1 hour, ≥99% after 4 hours, and ≥90% after 24 hours, achieving highly efficient, long-term, and stable hydrogen sulfide removal. Antibacterial tests show an inhibition rate of ≥99% against Escherichia coli, Staphylococcus aureus, and Salmonella, achieving a significant antibacterial effect. Mite killing tests show a mite kill rate close to or exceeding 100%, achieving effective mite eradication. In addition, comparative experiments have demonstrated that the combination of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj exhibits a certain synergistic effect in deodorization, antibacterial activity, and mite control.

[0054] 3. The composite biological deodorizer provided by this invention also contains humectants, surfactants, chelating agents, and flavoring agents. After 1 hour of spraying, the ammonia removal rate of this composite biological deodorizer reaches over 99%, and after 24 hours, the ammonia removal rate still reaches 90%, demonstrating strong biological ammonia removal capability and long-lasting ammonia removal effect. After 1 hour of spraying, the hydrogen sulfide removal rate of this composite biological deodorizer is ≥80%, and after 4 hours, the hydrogen sulfide removal rate is ≥99%. The deodorizing effect of this composite biological deodorizer is significantly higher than that of commercially available bio-enzyme deodorizers, plant extract deodorizers, and chlorine-containing disinfectant deodorizers, making it suitable for large-scale promotion and application.

[0055] 4. The composite biological deodorizer provided by this invention has a wide range of applications: it can be used as a deodorizer for pet supplies, pet feces, pet body odor, textile products, kitchen waste, home environment, car interior environment, farm environment or public toilet environment. Attached Figure Description

[0056] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0057] Figure 1 This is a colony morphology diagram of Lactobacillus brevis Lb-sj 001 provided by the present invention; Figure 2 This is a microscopic image of Lactobacillus brevis Lb-sj 001 provided by the present invention. Figure 3 The results of the inhibition zone experiment of Lactobacillus brevis Lb-sj 001 and commercially available Lactobacillus brevis against Escherichia coli in Example 5 of this invention; Figure 4 The results of the inhibition zone experiment of Lactobacillus brevis Lb-sj 001 and commercially available Lactobacillus brevis against Salmonella in Example 5 of this invention are as follows.

[0058] The present invention provides Lactobacillus brevis ( Lactobacillus brevis Lb-sj 001 was deposited on December 5, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36908.

[0059] Lactobacillus plantarum ( Lactobacillus plantarum LP-sj was deposited on August 15, 2014, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 9513. Detailed Implementation

[0060] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0061] Where specific experimental steps or conditions are not specified in the embodiments, they can be performed according to the conventional experimental steps or conditions described in the literature in this field. All raw materials or instruments used are commercially available conventional products, including but not limited to those used in the embodiments of this application.

[0062] Example 1: Isolation process of the strain The inventor isolated and screened a Gram-positive bacterial strain from a cheese sample, named Lb-sj 001. The isolation process was as follows: A cheese sample was taken, ground with an appropriate amount of physiological saline, and the grinding solution was diluted to different concentrations. 10... -6 10 -7 10 -8 Gradient bacterial suspensions were spread on MRS agar medium and incubated at 37 °C for 48 h. Larger colonies were picked and streaked on MRS agar plates for isolation. The colonies were purified repeatedly. The purified colonies were subjected to Gram staining, microscopic examination and physiological and biochemical tests. Gram-positive strains that were non-spore-forming and catalase-negative were selected, and the cultures were expanded and preserved in glycerol tubes and lyophilized tubes.

[0063] Composition and preparation process of MRS agar medium: Culture medium composition: peptone 10.0 g / L, beef extract 10.0 g / L, yeast extract 5.0 g / L, glucose 20.0 g / L, agar 15.0 g / L, sodium acetate 5.0 g / L, triammonium citrate 2.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate (MgSO4·7H2O) 0.1 g / L, manganese sulfate (MnSO4·4H2O) 0.05 g / L, Tween-80 1.0 mL.

[0064] Preparation process: Accurately weigh 70.00~70.15 g of MRS agar powder, add 1000 mL of distilled water, stir and heat to boiling to completely dissolve the powder. After cooling to room temperature, adjust the pH value to 6.2±0.2. Dispense the solution into Erlenmeyer flasks and autoclave at 121°C for 15 min. After the culture medium cools to about 45°C, pour it into sterilized Petri dishes under aseptic conditions, about 15 mL per dish, and let it stand to solidify.

[0065] like Figure 1 As shown, when cultured on MRS agar medium, the colonies are round, white, opaque, raised, and have a smooth surface. Figure 2 As shown, the bacteria are Gram-positive, rod-shaped, with rounded ends. Under a microscope, they can be observed to be approximately (1.0~1.2) μm × (2.5~7.0) μm in size. Most are non-motile, and some strains have thick capsules.

[0066] Example 2: Physiological and biochemical identification of the strain The biochemical identification of strain Lb-sj 001 was performed using API 50 CH strips (bioMérieux, France), and the results are shown in Table 1. The physiological and biochemical identification of strain Lb-sj 001 was performed using a kit (Qingdao Haibo Biotechnology), and the results are shown in Table 2.

[0067] Table 1. Biochemical identification results of strain Lb-sj 001 using API 50 CH.

[0068] Table 2. Physiological and biochemical characteristics of strain Lb-sj 001

[0069] The results in Tables 1 and 2 are consistent with the physiological and biochemical metabolic characteristics of the genus *Lactobacillus brevis*.

[0070] Example 3: Identification of 16S rRNA in strains Using universal primers 27F (5'-GTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') for the bacterial 16S rRNA gene, a 1423 bp gene fragment was obtained. The primer sequences are shown in SEQ ID No. 1 and SEQ ID No. 2 of the sequence listing. Comparison with known model bacteria using the EzbioCloud website (http: / / www.ezbiocloud.net / eztaxon / ) revealed that strain Lb-sj 001 showed 100% similarity to known strains Lactobacillus brevis (gene for 16S rRNA, partial sequence, strain: JCM 7758), Lactobacillus brevis strain LMT1-73 (chromosome, complete genome), and Lactobacillus brevis strain BD-LB (chromosome, complete genome).

[0071] The 16S rRNA sequence is shown in SEQ ID No. 3 of the sequence listing.

[0072] The sequence of SEQ ID No. 3 is as follows: The strain Lb-sj 001 was identified as *Lactobacillus brevis*. Lactobacillus brevis This strain has been deposited at the China General Microbiological Culture Collection Center (CGMCC), the designated depository of the State Intellectual Property Office. The address of the depository is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is December 5, 2025, and the deposit number is CGMCCNo. 36908.

[0073] Example 4: Determination of the deodorization ability of the strain I. Experimental Objective The ammonia removal and hydrogen sulfide removal capabilities of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj were quantitatively determined, respectively.

[0074] II. Experimental Samples Lactobacillus brevis Lb-sj 001 bacterial solution, commercially available Lactobacillus brevis bacterial solution, Lactobacillus plantarum LP-sj bacterial solution, commercially available Lactobacillus plantarum bacterial solution.

[0075] The above four bacterial suspensions were prepared using the following method: the bacterial strains were inoculated into MRS agar medium and cultured in an anaerobic incubator at 37 ℃ for 24 h to achieve a bacterial count of 2 × 10⁴. 8 cfu / mL.

[0076] Commercially available Lactobacillus brevis: Lactobacillus brevis SMHCC D11087, purchased from Shanghai Preservation Microbiology Co., Ltd.; Commercially available Lactobacillus plantarum: Lactobacillus plantarum LP28, 200 billion CFU / g, purchased from Xinlaiwang Biotechnology Co., Ltd.

[0077] III. Experimental Methods 1. Ammonia removal capacity test Add 5.0 g of cat litter containing a certain concentration of ammonia to a bottle containing 5 mL of bacterial solution, seal the bottle, and use an ammonia detector (model ZYKYCX, Shenzhen Zhengyuan Innovation Technology Co., Ltd.) to periodically detect the ammonia concentration in the bottle, and calculate the ammonia removal amount and ammonia removal rate.

[0078] 2. Determination of hydrogen sulfide removal capacity Weigh 0.2 g of ferrous sulfide and add 1 mL of 50% concentrated hydrochloric acid to prepare hydrogen sulfide gas. Introduce the prepared hydrogen sulfide into a 1 L bottle, add 5 mL of bacterial solution to the bottle beforehand, seal the bottle, and periodically detect the concentration of hydrogen sulfide in the bottle. Calculate the amount of hydrogen sulfide removed and the hydrogen sulfide removal rate.

[0079] IV. Experimental Results 1. Ammonia removal capacity Table 3. Ammonia removal efficiency of Lactobacillus brevis bacterial solution

[0080] Table 4. Ammonia removal effect of Lactobacillus plantarum bacterial solution

[0081] As shown in Tables 3 and 4, the ammonia removal effects of *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj treatment groups were significant within 1 hour of treatment, reaching 95.36% and 89.40%, respectively. In contrast, the ammonia removal rates of the two commercially available strains in the control group were 76.66% and 74.64%, respectively. After 24 hours of treatment, the ammonia removal rates of the *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj treatment groups remained at 78.80% and 84.51%, respectively, while the ammonia removal rates of the control group decreased to 52.63% and 56.33%, respectively. This demonstrates that both *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj possess strong ammonia removal capabilities, exhibit rapid onset and long-lasting effects, and their ammonia removal efficiency is significantly superior to that of commercially available strains.

[0082] 2. Hydrogen sulfide removal capability Table 5 Hydrogen sulfide removal efficiency of Lactobacillus brevis bacterial culture

[0083] Table 6 Hydrogen sulfide removal effect of Lactobacillus plantarum bacterial solution

[0084] As shown in Tables 5 and 6, the hydrogen sulfide removal effects of the *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj treatment groups were significant within 1 hour of treatment, reaching 50.00% and 51.02%, respectively. In contrast, the hydrogen sulfide removal rates of the two commercially available strains in the control group were 33.33% and 27.37%, respectively. After 4 hours of treatment, the hydrogen sulfide removal rates of the *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj treatment groups reached 80.00% and 81.63%, respectively, while the hydrogen sulfide removal rates of the control group were 51.04% and 44.21%, respectively. This demonstrates that both *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj have strong hydrogen sulfide removal capabilities, with significantly better results than commercially available strains.

[0085] Example 5: Determination of the antibacterial ability of the strain I. Experimental Objective The antibacterial effects of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Salmonella CVCC1809 (S. pullorum) were quantitatively determined.

[0086] II. Experimental Samples The following bacterial solutions were prepared according to Example 4: Lactobacillus brevis Lb-sj 001, commercially available Lactobacillus brevis, Lactobacillus plantarum LP-sj, and commercially available Lactobacillus plantarum.

[0087] III. Experimental Methods 1. Antibacterial rate The evaluation of antibacterial and bacteriostatic effects was conducted in accordance with WS / T 650-2019. After 15 minutes of action on pathogens, the inhibition rate was calculated (≥50% is considered effective, and ≥90% is considered highly effective) to scientifically determine the inhibitory ability of each strain against microorganisms.

[0088] 2. Antibacterial zone The antibacterial effect of Lactobacillus brevis Lb-sj 001 against Escherichia coli and Salmonella was detected by the spread plating method. 1) Cool the sterilized culture medium to about 45 ℃ and pour it out to allow it to solidify completely; 2) Dilute the indicator bacteria to a suitable concentration and spread 100 μL of the bacterial solution onto the surface of the culture medium; 3) Take out the prepared drug sensitivity tablets with tweezers, place them on the surface of the culture medium, and gently press them to make the tablets completely adhere to the culture medium. Mark them, and make two parallel samples for each group. Then place them in an incubator at 37 ℃ for 16 h. After the culture is completed, observe and record the results.

[0089] IV. Experimental Results 1. Antibacterial rate Table 7. Antibacterial rates of Lactobacillus brevis and Lactobacillus plantarum

[0090] As shown in Table 7, *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj exhibited significant antibacterial activity within 15 minutes of treatment, with inhibition rates of 81.00%–85.00% and 79.00%–86.00%, respectively, demonstrating potent antibacterial activity. In contrast, the two commercially available control strains showed inhibition rates of 66.00%–69.00% and 62.00%–65.00%, respectively, indicating only effective antibacterial activity. This demonstrates that both *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj possess strong antibacterial capabilities, with significantly better effects than commercially available strains.

[0091] 2. Antibacterial zone like Figure 3 As shown, Lactobacillus brevis Lb-sj 001 has a certain inhibitory effect on the indicator bacterium Escherichia coli. The diameters of the inhibition zones of the two parallel samples reached 17 mm and 18 mm, respectively (see mark B in the figure). The inhibition zones of the two parallel samples of commercially available Lactobacillus brevis were 10 mm and 12 mm, respectively (see mark A in the figure).

[0092] like Figure 4As shown, Lactobacillus brevis Lb-sj 001 has a good inhibitory effect on the indicator bacterium Salmonella. The diameters of the inhibition zones of the two parallel samples reached 33 mm and 32 mm, respectively (see G in the figure). The inhibition zones of the two parallel samples of commercially available Lactobacillus brevis were 15 mm and 16 mm, respectively (see H in the figure).

[0093] Example 6: Determination of the mite-killing ability of the strain I. Experimental Objective The ability of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj to kill mites was quantitatively determined.

[0094] II. Experimental Samples The following bacterial solutions were prepared according to Example 4: Lactobacillus brevis Lb-sj 001, commercially available Lactobacillus brevis, Lactobacillus plantarum LP-sj, and commercially available Lactobacillus plantarum.

[0095] III. Experimental Methods The acaricide test was conducted according to NY / T 1151.2-2006 "Indoor Efficacy Test Methods and Evaluation of Pesticide Registration Sanitary Insecticides Part 2: Acaricides and Acaricides": Four petri dishes were prepared, with 5 mL of bacterial solution sample placed in three of them, and no sample placed in the fourth petri dish (as a blank group). A mixture of white oil and petrolatum was evenly coated on the upper edge of the inner wall of each petri dish. 200 surviving mites were placed in the center of each petri dish. After 30 min, 0.05 g of mite feed was placed in the center of the petri dish. The petri dishes were then placed in a water-jacketed incubator for incubation. After 48 h, the number of dead mites was checked and recorded, and the acaricide rate was calculated.

[0096] IV. Experimental Results Table 8. Acaricidal effects of Lactobacillus brevis and Lactobacillus plantarum

[0097] As shown in Table 8, *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj achieved mite eradication rates of 69.67% and 67.00%, respectively, within 48 hours of mite treatment, while the control group of commercially available strains achieved rates of 48.17% and 43.00%, respectively. This demonstrates that both *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj possess strong mite-killing abilities and are significantly more effective than commercially available strains.

[0098] Example 7 Preparation of Compound Fermentation Broth This embodiment provides a method for preparing a compound fermentation broth of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj, the specific steps of which are as follows: S1. Preparation of seed solution: Lactobacillus brevis Lb-sj 001 was inoculated into MRS medium and cultured statically at 37 ℃ for 18 h to obtain Lactobacillus brevis seed culture with an OD value of 8.2. Lactobacillus plantarum LP-sj was inoculated into MRS medium and cultured statically at 37 ℃ for 18 h to obtain Lactobacillus plantarum seed culture with an OD value of 4.3. S2. Preparation of fermentation broth: a. Preparation of fermentation medium: Culture medium for fermenter No. 1: lactose 5%, peptone 1%, yeast powder 4%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, balance water, pH 5.5; sterilized at 121 ℃ for 15 min; Culture medium for fermenter No. 2: 5% glucose, 0.5% beef meal, 0.5% peptone, 2% yeast powder, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, with the remainder being water, pH 5.5; sterilized at 121 ℃ for 15 min. b. Fermentation culture Fermentation medium, accounting for 60% of the total volume, was added to fermenter No. 1. At a medium temperature of 30 °C, 5% (by volume) of *Lactobacillus brevis* seed culture was inoculated. The mixture was then incubated at 37 °C and 150 rpm with aeration and stirring for 24 h to achieve a viable count of 2.0 × 10⁻⁶. 9 The cfu / mL concentration yielded the Lactobacillus brevis fermentation broth. Fermentation medium, accounting for 60% of the total volume, was added to fermenter No. 2. At a medium temperature of 35–37 °C, 5% (by volume) of *Lactobacillus plantarum* seed culture was inoculated. The mixture was then incubated at 37 °C and 150 rpm with aeration and stirring for 24 h to achieve a viable count of 1.8 × 10⁻⁶ *Lactobacillus plantarum*. 10 The cfu / mL concentration yielded the Lactobacillus plantarum fermentation broth. S3. Preparation of compound fermentation broth: Mix *Lactobacillus brevis* fermentation broth and *Lactobacillus plantarum* fermentation broth evenly at a viable cell ratio of 1:1, 2:1, 3:1, or 1:2, controlling the total viable cell count to be 2 × 10⁻⁶. 8 The concentration of cfu / mL was introduced into a stirred tank and mixed until the pH value was close to 3.7, thus preparing a compound fermentation broth, which was subsequently named compound fermentation broth 1, compound fermentation broth 2, compound fermentation broth 3, and compound fermentation broth 4.

[0099] Example 8: Deodorization capacity test of compound fermentation broth I. Experimental Objective The ammonia removal and hydrogen sulfide removal capabilities of the combined fermentation broth of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj were quantitatively determined.

[0100] II. Experimental Samples Fermentation broths of *Lactobacillus brevis*, *Lactobacillus plantarum*, compound fermentation broth 1, compound fermentation broth 2, compound fermentation broth 3, and compound fermentation broth 4 were prepared according to Example 7, ensuring that the total viable count of each sample was 2 × 10⁻⁶. 8 cfu / mL.

[0101] III. Experimental Methods The experimental method is the same as in Example 4.

[0102] IV. Experimental Results 1. Ammonia removal capacity Table 9 Ammonia removal effect of compound fermentation broth

[0103] Table 10 Ammonia removal efficiency of single-strain fermentation broth

[0104] As shown in Tables 9 and 10, the ammonia removal rate of the combined fermentation broth of *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj exceeded 99% after 1 hour of treatment, and remained above 90% after 168 hours. This demonstrates the strong biological ammonia removal capacity and long-lasting effect of the combined fermentation broth, showing a significant ammonia removal effect. The ammonia removal rate varied depending on the ratio of the combined fermentation broth, with a 1:1 ratio of viable bacteria being the optimal result.

[0105] Comparative results of Lactobacillus brevis fermentation broth, Lactobacillus plantarum fermentation broth, and compound fermentation broth show that single-strain fermentation broth has a certain removal effect on ammonia in feces and urine, while the compound fermentation broth's ammonia removal effect is significantly better than that of single-strain fermentation broth. In terms of degradation pathways, the compound probiotic colonization and reproduction, along with multiple synergistic pathways, can continuously decompose organic nitrogen, decompose organic matter, and absorb small molecule nutrients. Single-strain probiotics show rapid initial effects but are prone to decline, while compound probiotics can establish a stable microecological chain, continuously inhibit the regeneration of harmful bacteria, and maintain a low pH environment through metabolic products (such as lactic acid), inhibiting ammonia volatilization and resulting in a more lasting effect.

[0106] 2. Hydrogen sulfide removal capability Table 11 Hydrogen sulfide removal effect of compound fermentation broth

[0107] Table 12 Hydrogen sulfide removal efficiency of single-strain fermentation broth

[0108] As shown in Tables 11 and 12, the hydrogen sulfide removal rate of the combined fermentation broth of *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj exceeded 80% after 1 hour of treatment, and generally reached over 99% after 4 hours. Even after 168 hours, the hydrogen sulfide removal rate remained above 90%, demonstrating the strong biological hydrogen sulfide removal capability and long-lasting effect of the combined fermentation broth. The hydrogen sulfide removal rate varied depending on the ratio of the combined fermentation broth, with a 1:1 ratio of viable bacteria being the optimal result.

[0109] Similar to its ammonia removal effect, the combined fermentation broth is significantly better at removing hydrogen sulfide than the single-strain fermentation broth.

[0110] Example 9: Determination of the antibacterial ability of the compound fermentation broth I. Experimental Objective The inhibitory effects of the combined fermentation broth of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj on Escherichia coli ATCC25922, Staphylococcus aureus ATCC 6538 and Salmonella CVCC1809 (S. pullorum) were quantitatively determined.

[0111] II. Experimental Samples Fermentation broths of *Lactobacillus brevis*, *Lactobacillus plantarum*, compound fermentation broth 1, compound fermentation broth 2, compound fermentation broth 3, and compound fermentation broth 4 were prepared according to Example 7, ensuring that the total viable count of each sample was 2 × 10⁻⁶. 8 cfu / mL.

[0112] III. Experimental Methods The experimental method is the same as in Example 5.

[0113] IV. Experimental Results Table 13 Antibacterial rate of compound fermentation broth

[0114] As shown in Table 13, the single-strain fermentation broths of *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj used in this invention exhibit certain antibacterial effects. The combined fermentation broth showed a significant enhancement in antibacterial activity within 15 minutes of treatment, with inhibition rates exceeding 99%, demonstrating potent antibacterial effects. The antibacterial mechanism is speculated to be as follows: competitive exclusion: occupying ecological niches and preventing harmful bacteria from attaching; acid production inhibition: secreting lactic acid, acetic acid, etc., to lower the environmental pH; and production of antibacterial substances: such as hydrogen peroxide and bacteriocins, directly killing pathogens.

[0115] Example 10: Determination of the mite-killing ability of the compound fermentation broth I. Experimental Objective The ability of a combined fermentation broth of Lactobacillus brevis Lb-sj 001 and Lactobacillus plantarum LP-sj to kill mites was quantitatively determined.

[0116] II. Experimental Samples Fermentation broths of *Lactobacillus brevis*, *Lactobacillus plantarum*, compound fermentation broth 1, compound fermentation broth 2, compound fermentation broth 3, and compound fermentation broth 4 were prepared according to Example 7, ensuring that the total viable count of each sample was 2 × 10⁻⁶. 8 cfu / mL.

[0117] III. Experimental Methods Refer to the experimental method in Example 6.

[0118] IV. Experimental Results Table 14. Acaricide Effect of Compound Fermentation Broth

[0119] As shown in Table 14, the single-strain fermentation broths of *Lactobacillus brevis* Lb-sj 001 and *Lactobacillus plantarum* LP-sj used in this invention have a certain acaricidal effect. The combined fermentation broth achieved a 100.00% acaricidal rate within 48 hours of treating mites, demonstrating a strong acaricidal effect. The acaricidal mechanism is speculated to be as follows: Competitive repulsion: Probiotics colonize the surface of experimental containers, competing with mites for nutrients and attachment sites, thereby reducing the mite's food source; Metabolic product acaricidal effect: Secretion of organic acids, bacteriocins, and other substances alters the local pH value, disrupting the suitable living environment for mites.

[0120] Example 11 Preparation of Composite Biological Deodorant This embodiment provides a method for preparing a composite biological deodorant, the specific steps of which are as follows: S1. Preparation of seed solution: Lactobacillus brevis Lb-sj 001 was inoculated into MRS medium and cultured statically at 37 ℃ for 18 h to obtain Lactobacillus brevis seed culture with an OD value of 8.2. Lactobacillus plantarum LP-sj was inoculated into MRS medium and cultured statically at 37 ℃ for 18 h to obtain Lactobacillus plantarum seed culture with an OD value of 4.3. S2. Preparation of fermentation broth: a. Preparation of fermentation medium: Culture medium for fermenter No. 1: lactose 5%, peptone 1%, yeast powder 4%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, balance water, pH 5.5; sterilized at 121 ℃ for 15 min; Culture medium for fermenter No. 2: 5% glucose, 0.5% beef meal, 0.5% peptone, 2% yeast powder, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, with the remainder being water, pH 5.5; sterilized at 121 ℃ for 15 min. b. Fermentation culture Fermentation medium, accounting for 60% of the total volume, was added to fermenter No. 1. At a medium temperature of 30 °C, 5% (by volume) of *Lactobacillus brevis* seed culture was inoculated. The mixture was then incubated at 37 °C and 150 rpm with aeration and stirring for 24 h to achieve a viable count of 2.0 × 10⁻⁶. 9 cfu / mL; Fermentation medium, accounting for 60% of the total volume, was added to fermenter No. 2. At a medium temperature of 37 ℃, 5% of *Lactobacillus plantarum* seed culture was inoculated. The mixture was then incubated at 37 ℃ and 150 rpm with aeration and stirring for 24 h to achieve a viable count of 1.8 × 10⁻⁶ *Lactobacillus plantarum*. 10 cfu / mL; S3. Preparation of compound fermentation broth: Mix Lactobacillus brevis fermentation broth and Lactobacillus plantarum fermentation broth evenly at a live cell ratio of 1:1, and then pour the mixture into a stirring tank to mix evenly. The pH value should be close to 3.7 to prepare the compound fermentation broth. S4. Preparation of compound biological deodorant: Compound fermentation broth (controlling the total viable bacteria count of the deodorant to 2×10⁻⁶). 8 The mixture consists of 5% glycerol, 1% poloxamer 188, 0.05% EDTA and 1.0% flavoring agent, with the balance being deionized water. Mix thoroughly to obtain the final product.

[0121] Example 12 Determination of the deodorizing ability of the compound biological deodorizer I. Experimental Objective This embodiment aims to compare the deodorizing effect of the composite biological deodorizer prepared in Example 11 with several commercially available deodorizer products.

[0122] II. Experimental Samples The compound biological deodorizer, biological enzyme deodorizer (product name: biological enzyme deodorizer, Hebei Fusai Biotechnology Development Co., Ltd.), plant extract deodorizer (product name: plant deodorizer, Dongguan Sanmei Chemical Technology Co., Ltd.), and chlorine-containing disinfectant deodorizer (product name: 5% sodium hypochlorite disinfectant, Hangzhou Langsuo Medical Disinfectant Co., Ltd.) prepared in Example 11.

[0123] III. Experimental Methods The experimental method is the same as in Example 4.

[0124] IV. Experimental Results 1. Ammonia removal capacity Table 15 Comparison of ammonia removal effects of different products

[0125] As shown in Table 15, the composite biological deodorizer treatment group provided by the present invention achieved an ammonia removal rate of over 99% after 1 hour of treatment, and still achieved an ammonia removal rate of over 90% after 24 hours. This demonstrates the powerful biological ammonia removal capability and long-lasting ammonia removal effect of the composite biological deodorizer, and its ammonia removal effect is significantly better than other types of commercially available products.

[0126] 2. Hydrogen sulfide removal capability Table 16 Comparison of hydrogen sulfide removal effects of different products

[0127] As shown in Table 16, the hydrogen sulfide removal rate of the composite biological deodorizer treatment group provided by the present invention reached 84.20% after 1 hour of treatment, and 99.0% after 4 hours. The hydrogen sulfide removal effect is significantly better than other types of commercially available products.

[0128] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A short lactobacillus, characterized in that, The specific *Lactobacillus brevis* is *Lactobacillus brevis* (… Lactobacillus brevis Lb-sj 001, with accession number CGMCC No. 36908.

2. A Lactobacillus brevis inoculum, characterized in that, Contains Lactobacillus brevis with accession number CGMCC No. 36908 ( Lactobacillus brevis )Lb-sj 001.

3. The use of the *Lactobacillus brevis* of claim 1 or the *Lactobacillus brevis* agent of claim 2 in the preparation of deodorants, antibacterial agents or acaricides.

4. The application according to claim 3, characterized in that, The deodorant is used to remove ammonia and / or hydrogen sulfide; The antibacterial agent is used to inhibit the growth and reproduction of at least one of the following bacteria: Escherichia coli, Staphylococcus aureus, and Salmonella.

5. A composite biological deodorizer, characterized in that, It contains Lactobacillus brevis and Lactobacillus plantarum, wherein the Lactobacillus brevis is specifically Lactobacillus brevis ( Lactobacillus brevis Lb-sj 001, with accession number CGMCC No. 36908, specifically refers to *Lactobacillus plantarum* (L. plantarum). Lactobacillus plantarum LP-sj, with accession number CGMCC No.9513.

6. The composite biological deodorizer according to claim 5, characterized in that, The ratio of viable bacteria of the *Lactobacillus brevis* to *Lactobacillus plantarum* is 1-3:1-2; The total viable bacteria count in the composite biological deodorant is not less than 2 × 10⁻⁶. 8 cfu / mL; The compound biological deodorant contains Lactobacillus brevis fermentation broth and Lactobacillus plantarum fermentation broth; Based on the mass percentage of the composite biological deodorizer, the composite biological deodorizer further contains at least one of the following components: 5%~15% humectant, 0.5%~1.5% surfactant, 0.02%~0.06% chelating agent, and 0%~5% flavoring agent, with the balance being deionized water; The humectant is glycerin and / or propylene glycol; the surfactant is poloxamer; and the chelating agent is ethylenediaminetetraacetic acid.

7. The method for preparing the composite biological deodorizer according to claim 5 or 6, characterized in that, Includes the following steps: S1. Preparation of seed culture: The Lactobacillus brevis was inoculated into MRS medium and cultured statically to obtain Lactobacillus brevis seed culture; The *Lactobacillus plantarum* was inoculated into MRS medium and allowed to stand for culture to obtain *Lactobacillus plantarum* seed culture. S2. Preparation of fermentation broth: The Lactobacillus brevis seed culture is inoculated into Lactobacillus brevis fermentation medium and fermented to obtain Lactobacillus brevis fermentation broth; The Lactobacillus plantarum seed culture is inoculated into Lactobacillus plantarum fermentation medium and fermented to obtain Lactobacillus plantarum fermentation broth; S3. Preparation of compound biological deodorant: The fermentation broth of Lactobacillus brevis and the fermentation broth of Lactobacillus plantarum are mixed evenly without the addition of excipients or with at least one of the following excipients: humectant, surfactant, chelating agent and flavor modifier, and the pH value of the mixture is adjusted to acidic to obtain the compound biological deodorant.

8. The method for preparing the composite biological deodorizer according to claim 7, characterized in that, In step S1, The static culture conditions for the Lactobacillus brevis are: static culture at 35~37 ℃ for 18~24 h; The static culture conditions for the *Lactobacillus plantarum* were: static culture at 35-37 ℃ for 18-24 h; In step S2, The fermentation culture conditions for *Lactobacillus brevis* are as follows: culture at 35-37 ℃ and 100-150 rpm with aeration and stirring for 18-24 h, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 1×10⁻⁶. 9 ~3×10 9 cfu / mL; The fermentation culture conditions for *Lactobacillus plantarum* are as follows: culture at 35-37 ℃ and 100-150 rpm with aeration and stirring for 18-24 hours, so that the viable count of the *Lactobacillus brevis* fermentation broth reaches 1×10⁻⁶. 10 ~2×10 10 cfu / mL; The inoculation amount of the Lactobacillus brevis seed culture is 5% to 10% of the volume of the Lactobacillus brevis fermentation medium; The inoculation amount of the *Lactobacillus plantarum* seed culture is 5% to 10% of the volume of the *Lactobacillus plantarum* fermentation medium; The amount of Lactobacillus brevis fermentation medium is 50% to 80% of the total volume of the fermenter; The sample volume of the Lactobacillus plantarum fermentation medium accounts for 50% to 80% of the total volume of the fermenter; The Lactobacillus brevis fermentation medium comprises the following components by weight percentage: lactose 5%–10%, peptone 1%–2%, yeast extract 4%–8%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.05%, and the balance being water; the pH value of the Lactobacillus brevis fermentation medium is 5.5–6.

0. The *Lactobacillus plantarum* fermentation medium comprises the following components by weight percentage: 5%–10% glucose, 0.5%–1% beef meal, 0.5%–1% peptone, 2%–5% yeast extract, 0.05% magnesium sulfate, 0.05% potassium dihydrogen phosphate, and the balance being water; the pH value of the *Lactobacillus plantarum* fermentation medium is 5.5–6.

0. In step S3, adjust the pH value to 2.5~4.

0.

9. The application of the composite biological deodorizer according to claim 5 or 6 or the composite biological deodorizer obtained by the preparation method according to claim 7 or 8 in deodorization, antibacterial or mite-killing.

10. The application according to claim 9, characterized in that, The deodorization process involves removing ammonia and / or hydrogen sulfide. The antibacterial effect is to inhibit the growth and reproduction of at least one of the following bacteria: Escherichia coli, Staphylococcus aureus, and Salmonella. The compound biological deodorizer is used as a deodorizer for pet supplies, pet feces, pet odor, textile products, kitchen waste, home environment, vehicle environment, farm environment, or public toilet environment.

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