Method for inducing, culturing and amplifying human natural killer cells
By extracting PBMCs from peripheral blood of healthy individuals and activating, inducing, and expanding them using specific culture media and activating factors, the problem of low in vitro proliferation rate of human natural killer cells was solved, achieving highly efficient NK cell culture with significantly improved cell purity and activity.
CN122104584APending Publication Date: 2026-05-29HANGZHOU QINGDA KERUI BIOTECHNOLOGY CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HANGZHOU QINGDA KERUI BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-03-13
- Publication Date
- 2026-05-29
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Figure CN122104584A_ABST
Abstract
The present application belongs to the field of cell culture. The present application provides a method for inducing, culturing and amplifying human natural killer cells, comprising the following steps: extracting peripheral blood from a healthy person, and separating PBMC; coating a container with an NK activation solution; culturing with an NK cell proliferation medium, inoculating PBMC, and incubating at a constant temperature for 1-6 days; and proliferating with an NK cell amplification medium, and incubating at a constant temperature for 1-10 days. The present application effectively improves the in-vitro proliferation rate of human natural killer cells. Through flow cytometry analysis, the proportion of NK cells with phenotypes of CD3, CD16 and CD56 in the cell population is all above 80%, and the cell phenotype is CD56 + CD3 ‑ NK cell group, and the positive rate is 97.57%. The present application has a simple composition formula, ideal culture effect, and wide application prospect.
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