Method for improving utilization efficiency of pelteobagrus fulvidraco feed by changing biological rhythm

By knocking out the rbmx gene in yellow catfish using CRISPR/Cas9 gene editing technology, designing specific sgRNAs, and screening homozygous mutants, the problem of improving feed utilization efficiency in traditional breeding methods has been solved, resulting in a significant improvement in feed utilization efficiency and green aquaculture for yellow catfish.

CN122104719APending Publication Date: 2026-05-29INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Application Number
CN202610106207.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-26
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies cannot quickly and effectively improve the feed utilization efficiency of yellow catfish through traditional breeding methods, and molecular marker-assisted selection is limited by natural genetic variation, making it difficult to achieve breakthrough improvements in traits.

Method used

By specifically knocking out the rbmx gene in yellow catfish using CRISPR/Cas9 gene editing technology, designing specific sgRNAs and modifying yellow catfish using a gene editing system, we obtained mutant alleles with loss of rbmx gene function and screened out homozygous yellow catfish mutants with high feed utilization efficiency.

Benefits of technology

It significantly improved the feed utilization efficiency of yellow catfish, increasing the number of female and male individuals by approximately 17.8% and 20.2% respectively, reducing aquaculture costs, decreasing aquaculture pollution, and promoting the green development of the industry.

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Abstract

The invention discloses a method for improving utilization efficiency of pelteobagrus fulvidraco feed by changing biological rhythm, and belongs to the technical field of fish genetic breeding. The invention provides a mutant allele for changing the biological rhythm of pelteobagrus fulvidraco, the mutant allele is obtained by performing targeted editing on a pelteobagrus fulvidraco rbmx gene coding region through a CRISPR / Cas9 technology, the sequence of the mutant allele is characterized in that a target region is subjected to frameshift mutation, so that protein translation is terminated in advance, and the mutant allele of the rbmx gene is specifically shown as SEQ ID NO: 2 or SEQ ID NO: 3. The homozygous pelteobagrus fulvidraco mutant containing the mutant allele provided by the invention shows that the feed utilization efficiency is remarkably improved, and female and male individuals are respectively improved by about 20.2% and 17.8% compared with wild individuals. The mutant allele and the pelteobagrus fulvidraco mutant can be used for breeding a new pelteobagrus fulvidraco strain with high feed utilization efficiency, and have important industrial application value.
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