A lignocellulose disassembly reagent for modifying lignin in situ, a high-quality lignin and lignocellulose disassembly method and a preparation method of glucose
By using a synergistic pretreatment system of PTSA, FeCl3, and PEG, the problem of lignin structure changes during the decomposition of lignocellulose was solved, achieving efficient glucose conversion and high-quality lignin preparation, and improving enzymatic hydrolysis efficiency and lignin structure stability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SOUTH CHINA AGRICULTURAL UNIVERSITY
- Filing Date
- 2026-01-13
- Publication Date
- 2026-05-29
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Abstract
Description
Technical Field
[0001] This invention relates to the field of chemical engineering technology, and more specifically, to an in-situ modified lignin lignocellulose dissociation reagent, high-quality lignin and lignocellulose dissociation method, and a method for preparing glucose. Background Technology
[0002] The massive consumption of fossil resources has caused severe environmental pollution and an energy crisis, forcing people to turn to clean, renewable alternative energy sources. Lignocellulose is the most abundant renewable resource in nature, making it the most promising alternative to fossil resources. In the cell walls of lignocellulose, the three main components—cellulose, hemicellulose, and lignin—are highly intertwined spatially and chemically, forming a dense yet chemically stable resilient structure. This resilient structure significantly hinders the efficient conversion of carbohydrates and the acquisition of high-quality lignin. Therefore, developing an efficient and controllable method for deconstructing lignocellulose is crucial.
[0003] In existing research, acid decomposition is widely used for the pretreatment of lignocellulose due to its high efficiency on hemicellulose and its advantages such as fast reaction rate, mature technology, and ease of scale-up. However, while these methods improve the efficiency of enzymatic hydrolysis, they often result in significant changes to the lignin structure and the formation of pseudolignin, which adversely affects subsequent enzymatic hydrolysis and lignin utilization.
[0004] Prior art 202110306301.7 discloses an acidic eutectic solvent and its preparation and application in improving enzymatic hydrolysis efficiency in pretreatment of straw. The acidic eutectic solvent is a 1:1 molar mixture of benzyltrimethylammonium chloride and p-toluenesulfonic acid, used to pretreat straw. The straw lignin removal rate is 75-84%, and the enzymatic saccharification efficiency is 89.2% at 15 FPU / g. This method uses a high concentration of p-toluenesulfonic acid for separation, which can easily cause equipment corrosion at high concentrations. Prior art 202410769779.7 discloses a method for preparing fermentable sugars from lignocellulose using recyclable low-concentration p-toluenesulfonic acid pretreatment, which can increase glucose yield to 81.55% to some extent. Both of these methods have limited glucose conversion efficiency and do not address lignin structure regulation or effective recycling. Summary of the Invention
[0005] To overcome the defects of the prior art described above, the present invention provides an in-situ modified lignin lignocellulose dissociation reagent.
[0006] The present invention also provides a method for disassembling lignocellulose.
[0007] The present invention also provides a high-quality lignin.
[0008] The present invention also provides a method for preparing glucose.
[0009] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows: A lignocellulose dissociation agent for in-situ modified lignin, comprising p-toluenesulfonic acid (PTSA), ferric chloride (FeCl3), polyethylene glycol (PEG) and water.
[0010] Preferably, in the in-situ modified lignin lignocellulose dissociation reagent, the amount of PTSA is 1% to 10% (mass percentage) of the lignocellulose raw material, the concentration of FeCl3 in the dissociation reagent is 0.1 to 0.2 mol / L, and the amount of PEG is 100 to 200 mg of PEG per gram of lignocellulose raw material.
[0011] Preferably, in the in-situ modified lignin lignocellulose dissociation reagent, the amount of PTSA is 5.5%~10% (mass percentage) of the lignocellulose raw material, the concentration of FeCl3 in the dissociation reagent is 0.1~0.2 mol / L, and the amount of PEG is 100~200 mg of PEG per gram of lignocellulose raw material.
[0012] More preferably, in the in-situ modified lignin lignocellulose dissociation reagent, the amount of PTSA is 10% (mass percentage) of the lignocellulose raw material, the concentration of FeCl3 in the dissociation reagent is 0.1~0.2 mol / L, and the amount of PEG is 100~200 mg of PEG per gram of lignocellulose raw material.
[0013] Preferably, the average molecular weight of the PEG is 1800~8000 Da.
[0014] Preferably, the PEG includes one of PEG2000, PEG6000, and PEG8000.
[0015] More preferably, the PEG includes one of PEG6000 and PEG8000.
[0016] A method for dismantling lignocellulose involves reacting the lignocellulose raw material with the in-situ modified lignin lignocellulose dismantling reagent to achieve dismantling.
[0017] Preferably, the method for dismantling lignocellulose includes the following steps: placing the lignocellulose raw material in the in-situ modified lignin lignocellulose dismantling reagent for mixing and reaction, and performing solid-liquid separation after the reaction to obtain filtrate and solid portion, thus completing the dismantling.
[0018] Preferably, in the method for disassembling lignocellulose, the lignocellulose raw materials include coniferous wood, broadleaf wood, and grasses.
[0019] Preferably, the grasses include corn stalks and wheat stalks.
[0020] Preferably, in the method for dissolving lignocellulose, the ratio of lignocellulose raw material to lignocellulose dissolving reagent for in-situ modified lignin is 1g:10-15mL. More preferably, the ratio of lignocellulose raw material to lignocellulose dissolving reagent for in-situ modified lignin is 1g:10mL.
[0021] Preferably, in the method for disintegrating lignocellulose, the reaction conditions are a temperature of 110~130℃.
[0022] Preferably, in the method for disintegrating lignocellulose, the reaction conditions are 40-80 min.
[0023] Preferably, the lignocellulose raw material is pulverized and sieved to 40-60 mesh.
[0024] A method for preparing glucose involves reacting lignocellulose raw material with a lignocellulose dissociation reagent of in-situ modified lignin, and then enzymatically hydrolyzing the solid portion to obtain the glucose.
[0025] The enzymatic hydrolysis process includes the following steps: adding cellulase and buffer solution to the solid portion obtained by the disassembly method for reaction.
[0026] Preferably, in the enzymatic hydrolysis treatment, the amount of cellulase added is 4~12 FPU / g.
[0027] Preferably, in the enzymatic hydrolysis treatment, the amount of cellulase added is 4~8 FPU / g.
[0028] Preferably, in the enzymatic hydrolysis treatment, the amount of cellulase added is 4~6 FPU / g.
[0029] Preferably, the pH value of the buffer solution is 4 to 6.
[0030] Preferably, the pH value of the buffer solution is 4.8.
[0031] Preferably, the buffer solution includes a citrate buffer solution, which is a mixture of citric acid monohydrate and trisodium citrate dihydrate.
[0032] Preferably, the mass-to-volume ratio of the obtained solid portion to the buffer solution is 1 g: 40~60 mL.
[0033] Preferably, the mass-to-volume ratio of the obtained solid fraction to the buffer solution is 1 g: 50 mL.
[0034] Preferably, the enzymatic hydrolysis is performed at a temperature of 40-60°C for 68-76 hours.
[0035] Preferably, the enzymatic hydrolysis temperature is 50°C.
[0036] A high-quality lignin is obtained by reacting lignocellulose raw material with a lignocellulose disintegrating reagent of the in-situ modified lignin, obtaining a solid part, enzymatically hydrolyzing it, and then extracting and separating the reaction system after enzymatic hydrolysis.
[0037] Preferably, the high-quality lignin has a PEG grafted to the α site of the lignin β-O-4 bond to form an etherified structure.
[0038] Further, the extraction and separation includes the following steps: solid-liquid separation of the reaction system after enzymatic hydrolysis, collection of precipitate and washing to neutrality to obtain crude lignin; drying the crude lignin and mixing it with a dioxane / water mixed solvent, centrifuging to obtain the supernatant; concentrating the supernatant and adding an aqueous acetic acid solution, and further adding distilled water to induce precipitation, separating the precipitate and drying it to obtain high-quality lignin.
[0039] Preferably, the centrifugal speed is 8000-10000 rpm.
[0040] Preferably, the stirring includes stirring in the dark.
[0041] Preferably, the stirring speed is 300~500 rpm and the stirring time is 22~26 hours.
[0042] Preferably, in the extraction process, the operation of drying the crude lignin and mixing it with a dioxane / water mixed solvent can be performed once or more, preferably three times.
[0043] In this invention, the lignin obtained by the above preparation method is high-quality lignin. During the pretreatment and disassembly process, polyethylene glycol participates in the regulation of lignin structure, giving the obtained lignin the structural characteristics of PEG modification.
[0044] This invention proposes a ternary synergistic pretreatment technology based on PTSA, FeCl3, and PEG, which achieves efficient deconstruction of lignocellulose structure and in-situ regulation of lignin structure under mild and controllable conditions. This technology overcomes the technical limitation of traditional acid pretreatment methods, where "increasing sugar yield is often accompanied by lignin condensation," and achieves efficient deconstruction of lignocellulose biomass and in-situ modification of lignin while significantly improving enzymatic hydrolysis efficiency.
[0045] In this invention, a ternary synergistic pretreatment system of PTSA, FeCl3, and PEG is used, which can significantly improve the enzymatic conversion efficiency even with a low amount of cellulase added, with a glucose yield of up to 97.9%. This provides an efficient and controllable technical route for the resource utilization of lignocellulose biomass.
[0046] This invention addresses the reaction characteristics of the p-toluenesulfonic acid pretreatment system during the decomposition of lignocellulose. It proposes a core technical solution based on synergistic system regulation. Through comprehensive design of the PTSA acidic environment, the action of FeCl3 metal salt, and the participation of PEG polymers, it achieves effective decomposition while preventing lignin structure condensation. This solution utilizes the synergistic effects of each component in the p-toluenesulfonic acid system to comprehensively regulate hemicellulose removal, lignin structural stability, and surface properties. This results in improved cellulose hydrolysis and lignin structural integrity while maintaining efficient decomposition, achieving a treatment effect that balances high conversion efficiency with controllable lignin structure.
[0047] This invention modifies lignin in situ during the pretreatment stage, enabling it to maintain high structural stability during disintegration and enzymatic hydrolysis. This results in high-quality lignin with high structural integrity even after enzymatic hydrolysis. Compared to conventional techniques that prioritize lignin removal followed by recycling, this invention effectively preserves the lignin structure while achieving efficient conversion, demonstrating significant technical advantages.
[0048] Compared with the prior art, the beneficial effects of the technical solution of the present invention are: This invention achieves effective removal of hemicellulose while significantly improving glucose yield during enzymatic hydrolysis, and can obtain high-quality lignin with a high yield. Through the synergistic effect of PTSA, FeCl3, and PEG, high enzymatic hydrolysis efficiency can be obtained with a low amount of cellulase added. The glucose yield of this invention can reach up to 97.9%, and the low amount of cellulase added helps to reduce the cost of the enzymatic hydrolysis process. This technical solution shows significant advantages in balancing improved sugar yield and lignin structure regulation, and is suitable for the efficient and comprehensive utilization of lignocellulose. Attached Figure Description
[0049] Figure 1 The HSQC data graphs for lignin in Comparative Example 5 and Example 4 are shown.
[0050] Figure 2 This is a microscopic structural diagram of the raw material and the solid part after disassembly. Detailed Implementation
[0051] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.
[0052] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.
[0053] The corn stalks (grass) are sourced from Lianyungang City and are obtained through air drying, crushing, and sieving. The corn stalks contain 33.85% cellulose, 19.29% hemicellulose, 20.63% lignin, and 2.98% ash.
[0054] Pine (coniferous wood) has a cellulose content of 51.23%, a hemicellulose content of 10.47%, and a lignin content of 27.62%.
[0055] Poplar (broadleaf wood) has a cellulose content of 45.56%, a hemicellulose content of 14.98%, and a lignin content of 22.86%.
[0056] Wheat straw (a type of grass in the Poaceae family) contains 35.13% cellulose, 20.52% hemicellulose, and 20.16% lignin.
[0057] Analytical grade p-TsOH (PTSA) of p-toluenesulfonic acid was purchased from Tianjin Damao; analytical grade FeCl3 was purchased from Maclean's; chemically pure PEG8000 was purchased from Yuanye Biotechnology, with an average molecular weight of 8000; analytical grade PEG6000 was purchased from Maclean's, with an average molecular weight of 6000; experimental reagent PEG2000 was purchased from Tianjin Damao, with an average molecular weight of 1800-2200; analytical grade PEG200 was purchased from Maclean's, with an average molecular weight of 200; cellulase Cellic CTec 2 was purchased from Novozymes (Beijing, China), with a cellulase activity of 138 FPU / mL.
[0058] Example 1 Corn stalk dismantling: Crush the corn stalks and sieve them to 40-60 mesh to obtain crushed corn stalks. Mix the crushed corn stalks with the dismantling reagent at a solid-liquid ratio of 1 g: 10 mL, and keep it at 120℃ for 60 min in a high-pressure reactor. Then, filter the mixture using a nylon filter cloth (400 mesh) to obtain the filtrate and solid fraction. Wash the solid fraction with pure water until the pH is neutral (pH=6-8).
[0059] Enzymatic hydrolysis: Take 1 g of the solid part obtained from disassembly (based on oven-dry weight) and mix it with 50 mL of citrate buffer (pH 4.8), then add 5 FPU / g of cellulase (i.e., add 5 FPU of cellulase per g of solid part), and place the resulting mixture in a 50℃ incubator for enzymatic hydrolysis reaction for 72 hours.
[0060] Lignin Extraction: The solid fraction was subjected to two enzymatic hydrolysis treatments, each lasting 72 hours. The reaction system was then centrifuged at 6000 rpm to collect the precipitate. The precipitate was washed with water until the pH was neutral to obtain crude lignin. The crude lignin was vacuum dried and then mixed with a dioxane / water mixed solvent (dioxane to water volume ratio 96:4) at a solid-liquid ratio of 1:10 at room temperature. The mixture was stirred at 500 rpm for 24 hours in the dark, and the supernatant was collected by centrifugation at 10000 rpm. This mixing and stirring process was repeated three times. The supernatants from the three extractions were combined and concentrated by rotary evaporation. After the solvent was evaporated, the concentrate was added to a 90% acetic acid aqueous solution (concentrate to 90% acetic acid aqueous solution ratio of 1 g: 5 mL), and then distilled water was added to induce precipitation. The resulting precipitate was separated by centrifugation at 10000 rpm and freeze-dried to obtain high-quality lignin.
[0061] In this embodiment, the disintegration reagent is obtained by mixing PTSA, FeCl3, PEG8000 and water. The amount of PTSA is 1% (mass percentage) of the mass of the crushed corn stalks, the concentration of FeCl3 in the disintegration reagent is 0.2 mol / L, and the amount of PEG8000 is 100 mg / g (100 mg of PEG8000 per gram of crushed corn stalks).
[0062] Examples 2-6 The technical solutions of Examples 2 to 6 are similar to those of Example 1, except that the amounts of PTSA, FeCl3, and PEG8000 in the disintegration reagents are different, as detailed in Table 1.
[0063] Table 1
[0064] Example 7 The technical solution of Example 7 is similar to that of Example 5, except that PEG2000 is used instead of PEG8000 in the disintegration reagent.
[0065] Example 8 The technical solution of Example 8 is similar to that of Example 5, except that PEG6000 is used instead of PEG8000 in the disintegration reagent.
[0066] Example 9 The technical solution of Example 9 is similar to that of Example 1, except that the raw material used in this example is pine wood; Disassembly of pine wood: Pulverized pine wood (crushed and sieved to 40-60 mesh) is mixed with disassembly reagent at a solid-liquid ratio of 1g:15mL and kept at 110℃ for 80 min in a high-pressure reactor. Then, the mixture is filtered through nylon filter cloth (400 mesh) to obtain the filtrate and solid fraction. The solid fraction is then washed with pure water until the pH is neutral (pH=6-8).
[0067] Enzymatic hydrolysis: Take 1 g of the solid part obtained from disassembly (based on oven-dry weight) and mix it with 40 mL of citrate buffer (pH 6), then add cellulase 4 FPU / g. Place the resulting mixture in an incubator at 45 ℃ for enzymatic hydrolysis reaction for 76 hours.
[0068] Lignin Extraction: The solid fraction was subjected to two enzymatic hydrolysis treatments. The reaction system was centrifuged at 8000 rpm to collect the precipitate. The precipitate was washed with water until the pH was neutral to obtain crude lignin. The crude lignin was vacuum dried and then mixed with a dioxane / water mixed solvent (dioxane to water volume ratio 96:4) at a solid-liquid ratio of 1:10 at room temperature. The mixture was stirred at 300 rpm for 24 hours in the dark, and the supernatant was collected by centrifugation at 6000 rpm. The above mixing and stirring operation in the dark was repeated three times. The supernatants obtained from the three extractions were combined and concentrated by rotary evaporation. The concentrate was added to a 90% acetic acid aqueous solution (the ratio of concentrate to 90% acetic acid aqueous solution was 1 g: 5 mL), and then distilled water was added to induce precipitation. The precipitate was separated by centrifugation at 6000 rpm and freeze-dried to obtain high-quality lignin.
[0069] Example 10 The technical solution of Example 10 is similar to that of Example 1, except that poplar wood is used as the raw material in this example. Poplar wood dismantling: The crushed poplar wood (crushed and sieved to 40-60 mesh) is mixed with the dismantling reagent at a solid-liquid ratio of 1:15 and kept at 130℃ for 40 min in a high-pressure reactor. Then, the mixture is filtered through a nylon filter cloth (400 mesh) to obtain the filtrate and solid fraction. The solid fraction is then washed with pure water until the pH is neutral (pH=6-8).
[0070] Enzymatic hydrolysis: Take 1 g of the solid part obtained from disassembly (based on oven-dry weight) and mix it with 60 mL of citrate buffer (pH4), then add 8 FPU / g of cellulase. Place the resulting mixture in a 55 ℃ incubator for enzymatic hydrolysis for 76 hours.
[0071] Lignin Extraction: The solid fraction was subjected to two enzymatic hydrolysis treatments. The reaction system was centrifuged at 10,000 rpm to collect the precipitate. The precipitate was washed with water until the pH was neutral to obtain crude lignin. The crude lignin was vacuum dried and then mixed with a dioxane / water mixed solvent (dioxane to water volume ratio 96:4) at a solid-liquid ratio of 1:10 at room temperature. The mixture was stirred at 300 rpm for 24 hours in the dark, and the supernatant was collected by centrifugation at 8,000 rpm. The above mixing and stirring operation in the dark was repeated three times. The supernatants obtained from the three extractions were combined and concentrated by rotary evaporation. The concentrate was added to a 90% acetic acid aqueous solution (the ratio of concentrate to 90% acetic acid aqueous solution was 1 g: 5 mL), and then distilled water was added to induce precipitation. The precipitate was separated by centrifugation at 6,000 rpm and freeze-dried to obtain high-quality lignin.
[0072] Example 11 The technical solution of Example 11 is similar to that of Example 5, except that the raw material used in this example is wheat straw; the amount of cellulase added in the enzymatic hydrolysis treatment is different, with cellulase 12 FPU / g.
[0073] Comparative Examples 1-7 The technical solutions of Comparative Examples 1 to 7 are similar to those of Example 5, except that the amounts of PTSA, FeCl3, and PEG8000 in the disintegration reagents are different, as shown in Table 2.
[0074] Table 2
[0075] Comparative Example 8 The technical solution of Comparative Example 8 is similar to that of Example 5, except that PEG200 is used instead of PEG8000 in the disintegration reagent.
[0076] The surface morphology and microstructure characteristics of the samples were clearly observed using a scanning electron microscope (SEM; S4800, Hitachi, Tokyo).
[0077] The 2D-HSQC of different lignins was determined using a Bruker AVIII 600 MHz nuclear magnetic resonance spectrometer. 13 C / 1 H) spectrum was used to analyze the changes in the β-O-4 structure in lignin.
[0078] After enzymatic hydrolysis, the supernatant was collected, heated at 100°C for 5 minutes, and centrifuged at 10,000 rpm for further sugar analysis. The formula for calculating glucose yield is as follows:
[0079] N is the measured glucose concentration (g / L); V is the volume of the enzymatic hydrolysis system (L); Among them, 0.9 is the dehydration coefficient when glucose is converted into glucan.
[0080] The composition of all samples was measured according to the NREL method, and the products were determined by high performance liquid chromatography (HPLC). Glucose and xylose were prepared using a sugar column (SH1011, Shodex), and the flow rate of the mobile phase (5mM H2SO4) was 0.5 mL / min (50℃).
[0081] The results are shown in the table below: Table 3 Table 3. Data for Examples and Comparative Cases
[0082] Analysis and Explanation: Analysis of the results in Table 3 shows that, as indicated in Comparative Examples 1-3, the hemicellulose removal rate and glucose yield were low (glucose yield ≤ 40.60%) when the disintegrating reagent contained both PTSA and PEG. Comparative Examples 4-6 show that the hemicellulose removal rate increased when the disintegrating reagent contained both PTSA and FeCl3, with glucose yields ranging from 47.53% to 55.63%, indicating that the synergistic effect of PTSA and FeCl3 promotes hemicellulose removal. Comparative Example 7 shows that when the disintegrating reagent contained only PTSA, the hemicellulose removal was low, and the glucose yield was only 16.6%. The results of Comparative Examples 1-7 indicate that the overall treatment effect of disintegrating reagent systems containing PTSA or a binary combination of PTSA and FeCl3 is limited, resulting in low glucose yields.
[0083] The lignocellulose decomposition and conversion method provided by this invention achieves hemicellulose removal rates between 78.06% and 90.99%; glucose yield can reach up to 97.9% (Example 4); and lignin retention rates between 82.46% and 88.96%. Figure 1 It is evident that PEG8000 can form an etherified structure by grafting onto the α site of the β-O-4 bond in lignin, effectively inhibiting the condensation reaction between lignin molecules. In particular, the β-O-4 bond content in Example 4 is higher than that in the PTSA / FeCl3 pretreated group (Comparative Example 5), increasing from 15.31% (Comparative Example 5) to 23.14% (Example 4). The high-quality lignin obtained in this way not only maintains a high recovery rate but also has a high β-O-4 bond content. The modified site only has the α site of the β-O-4 bond grafted onto the lignin to form an etherified structure, which is beneficial for subsequent high-value utilization and also reduces its adverse effects on cellulase during enzymatic hydrolysis.
[0084] Depend on Figure 2As can be seen from SEM observation, after treatment with PTSA and FeCl3 disintegrating reagents (Comparative Example 5), a layered exfoliation structure appeared on the surface of the straw, accompanied by pseudolignin deposition, which enhances the non-productive adsorption sites of cellulase. The presence of PTSA and FeCl3... 3、 After treatment with PEG8000 disintegration reagent (Example 4), the deposition of pseudolignin was significantly inhibited. Unreacted PEG particles (with blurred boundaries) remained on the surface, effectively modifying the surface properties of lignin and restoring the surface of straw to smoothness.
[0085] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.
Claims
1. A lignocellulose dissociation reagent for in-situ modified lignin, characterized in that, Its components include p-toluenesulfonic acid, ferric chloride, polyethylene glycol, and water.
2. The lignocellulose dissociation reagent for in-situ modified lignin according to claim 1, characterized in that, The amount of p-toluenesulfonic acid used is 1% to 10% of the mass of the lignocellulose raw material, the concentration of FeCl3 in the disintegration reagent is 0.1 to 0.2 mol / L, and the amount of polyethylene glycol used is 100 to 200 mg of polyethylene glycol per gram of lignocellulose raw material.
3. The lignocellulose dissociation reagent for in-situ modified lignin according to claim 1, characterized in that, The average molecular weight of the polyethylene glycol is 1800~8000 Da.
4. A method for disassembling lignocellulose, characterized in that, The lignocellulose raw material is decomposed by reacting it with the lignocellulose disintegrating reagent of any one of claims 1 to 3.
5. The method for disassembling lignocellulose according to claim 4, characterized in that, The ratio of lignocellulose raw material to lignocellulose disintegration reagent for in-situ modified lignin is 1g:10~15mL.
6. The method for disassembling lignocellulose according to claim 4, characterized in that, The reaction conditions are a temperature of 110~130℃.
7. A method for preparing glucose, characterized in that, The product is prepared by reacting lignocellulose raw material with the lignocellulose dissociation reagent of in-situ modified lignin as described in any one of claims 1 to 3, and then enzymatically hydrolyzing the solid portion. The enzymatic hydrolysis process includes the following steps: adding cellulase and buffer solution to the solid portion obtained by the disassembly method for reaction.
8. The method for preparing glucose according to claim 7, characterized in that, The amount of cellulase added is 4~12 FPU / g.
9. The method for preparing glucose according to claim 7, characterized in that, The enzymatic hydrolysis is performed at a temperature of 40-60℃ for 68-76 hours.
10. A high-quality lignin, characterized in that, The lignocellulose raw material is reacted with the lignocellulose dissociation reagent of in-situ modified lignin as described in any one of claims 1 to 3, and the solid part is subjected to enzymatic hydrolysis. The reaction system after enzymatic hydrolysis is then extracted and separated to obtain the final product.