A homogeneous chemiluminescent kit for detecting platelet-monocyte aggregates and a method for detecting the same
By using homogeneous chemiluminescence to detect PMA, and utilizing the formation of ortho-complexes between CD41 and CD14 antibodies and DNA conjugates, the complexity and high cost of PMA detection in existing technologies are solved, enabling rapid and accurate PMA detection that is suitable for the clinical diagnosis of sepsis.
CN122109516AActive Publication Date: 2026-05-29NANJING POCLIGHT BIOTECHNOLOGY CO LTD +1
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- NANJING POCLIGHT BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-04-28
- Publication Date
- 2026-05-29
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Figure CN122109516A_ABST
Abstract
The application belongs to the field of biological medicine, and particularly relates to a homogeneous chemiluminescence kit for detecting platelet-monocyte aggregates and a detection method thereof. The kit comprises: R1 reagent: CD41 antibody-DNA1 conjugate; R2 reagent: CD14 antibody-DNA2 conjugate; R3 reagent: DNA3 labeled with acridinium ester; and R4 reagent: antioxidant combined with graphene oxide. The application realizes direct whole blood detection of platelet-monocyte aggregates in 5-10 minutes through homogeneous chemiluminescence+orthogonal DNA hybridization+special sample stabilizer, greatly simplifies the process, reduces the cost, and enables the sample to be stable at room temperature for 6 hours. The detection result is highly correlated with flow cytometry, and has good diagnostic efficiency for sepsis.
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