An integrated system and method for inducing oxygen poisoning modeling, anesthesia, and sample collection in rats.

Abstract

This invention relates to an integrated system and method for modeling, anesthesia, and material collection in mice using oxygen poisoning: Experimental preparation; Animal placement: Two mice are placed in cages within two separate modeling chambers, and all valves are closed; High-pressure environment establishment: The main oxygen supply electric valve and the first and second oxygen supply electric valves are opened, and the first and second pressure regulating valves are activated, allowing high-pressure oxygen to flow into the first and second modeling chambers to form a high-pressure oxygen environment; Modeling monitoring: The state of the mice in each modeling chamber is recorded from the start of modeling. If a mouse in any modeling chamber exhibits a high-pressure oxygen convulsion state, the high-pressure oxygen poisoning model is successfully established; Post-modeling anesthesia: After the mouse model is successfully established in the modeling chamber, the corresponding flow controller is activated to allow the anesthetic to flow into the modeling chamber, bringing the mouse to a state of deep anesthesia; Independent material collection preparation: The operator enters a human diving simulation training chamber and controls the environment to reach preset parameters; Material collection operation: The operator removes the deeply anesthetized mouse from the modeling chamber and places it on the material collection platform for material collection.

Description

Technical Field

[0001] This invention relates to the field of animal experimental equipment technology, and in particular to an integrated system for mouse oxygen poisoning modeling, anesthesia and material collection that is connected to a human diving simulation training chamber, as well as a method for mouse hyperbaric oxygen poisoning modeling, anesthesia and material collection, which is suitable for rodent experimental research under high pressure environment. Background Technology

[0002] In the field of diving medicine research, hyperbaric oxygen poisoning is a common symptom in hyperbaric environments, seriously threatening the lives of divers. To systematically explore its pathogenesis, pathological characteristics, and prevention strategies, a standardized oxygen convulsion model needs to be constructed through animal experiments. Rodents, due to their physiological homology with humans, are commonly used experimental subjects in this field. Current methods for establishing hyperbaric oxygen poisoning models in rodents mostly use independent hyperbaric chambers, which have four significant drawbacks: First, removing the rodents from the hyperbaric environment after modeling can cause sudden changes in environmental parameters, easily triggering stress responses and interfering with the stability of physiological indicators. Second, the timing of anesthesia and the sampling process are not well-coordinated; existing devices require removing the rodents from the chamber before anesthesia. Third, existing devices lack closed-environment monitoring mechanisms, making timely intervention impossible in case of experimental abnormalities, increasing animal mortality and experimental losses. Fourth, most devices are single-chamber designs, making it impossible to model multiple rodents simultaneously; some devices place multiple rodents in a single chamber simultaneously, and when modeling times are inconsistent, the sampling process interferes with each other, reducing experimental efficiency.

[0003] This invention aims to solve four core problems of existing devices: the experimental results are affected when animals leave the high-pressure environment after modeling and are taken out for material collection; anesthesia cannot be performed immediately after modeling; there is a lack of real-time monitoring and emergency treatment of environmental information; and multiple mice cannot be modeled and collected independently. Summary of the Invention

[0004] This invention addresses the problems and shortcomings of existing technologies by providing an integrated system and method for creating, anesthetizing, and collecting samples from rats using oxygen poisoning, which is connected to a human diving simulation training chamber.

[0005] The present invention solves the above-mentioned technical problems through the following technical solution:

[0006] This invention provides an integrated system for modeling, anesthesia, and tissue collection in rats using oxygen poisoning. Its key feature is that it comprises an integrated dual-independent modeling chamber placed within a human diving simulation training chamber. Each dual-independent modeling chamber includes a pressure-resistant chamber body. A vertical partition is fixed in the middle of the pressure-resistant chamber body to divide it into a first modeling chamber and a second modeling chamber. A perforated cage capable of supporting one rat is detachably fixed within both the first and second modeling chambers. The first and second modeling chambers are respectively connected to one end of a first oxygen supply branch pipe and one end of a second oxygen supply branch pipe. The other ends of both the first and second oxygen supply branch pipes are connected to one end of a main oxygen supply pipe. The other end of the main oxygen supply pipe is connected to a high-pressure oxygen cylinder. A main oxygen supply electric valve is installed on the main oxygen supply pipe. A first oxygen supply electric valve and a first pressure regulating valve are installed on the first oxygen supply branch pipe. A second oxygen supply electric valve and a second pressure regulating valve are installed on the second oxygen supply branch pipe. A small temperature regulator, a small humidity regulator, a pressure sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor, a temperature sensor, a humidity sensor, and a high-definition camera are installed on both the first and second modeling chambers. A control box is fixed to the side wall of the first or second modeling chamber. The control box integrates a main controller and has a display screen and an alarm embedded in it.

[0007] The first and second modeling chambers are each connected to a respiratory anesthesia device. Each respiratory anesthesia device includes an anesthetic vaporizer. A flow controller and an anesthetic concentration sensor are sequentially installed on the pipeline between the anesthetic vaporizer and the corresponding modeling chamber.

[0008] A material handling platform is installed on the top of the dual independent molding chambers;

[0009] The main controller is used to import preset environmental parameters corresponding to the target diving depth of the human diving simulation training chamber, including preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range, and preset humidity range. It controls the main oxygen supply electric valve, the first oxygen supply electric valve, and the second oxygen supply electric valve to open, and simultaneously starts the first pressure regulating valve and the second pressure regulating valve. High-pressure oxygen from the high-pressure oxygen cylinder flows into the first modeling chamber and the second modeling chamber respectively, so that the pressure detected by the pressure sensor in each modeling chamber reaches the preset pressure range, and the oxygen concentration detected by the oxygen concentration sensor reaches the preset oxygen concentration range, forming a high-pressure oxygen environment. At the same time, it controls the small temperature regulator to start to achieve temperature regulation, so that the temperature detected by the temperature sensor in each modeling chamber returns to the preset temperature range, and controls the small humidity regulator to start to achieve humidity regulation, so that the humidity detected by the humidity sensor in each modeling chamber returns to the preset humidity range.

[0010] The main controller is also used to receive the state of the mice in the corresponding modeling chamber recorded by each high-definition camera in real time from the start of modeling, analyze the state of the mice in each modeling chamber, and when a mouse in any modeling chamber exhibits hyperbaric oxygen convulsion, the modeling is completed due to hyperbaric oxygen poisoning, and the corresponding oxygen supply electric valve of the modeling chamber is closed. If mice in two modeling chambers exhibit hyperbaric oxygen convulsion and modeling is completed, all oxygen supply electric valves and the main oxygen supply electric valve are closed.

[0011] The main controller is also used to activate the corresponding flow controller after the mouse hyperbaric oxygen convulsion model is successfully established in the modeling chamber, so that the anesthetic in the anesthetic vaporizer flows into the modeling chamber to induce respiratory anesthesia in the mouse, monitor the anesthetic concentration fed back by the anesthetic concentration sensor, analyze the state of the mouse in the modeling chamber, and send a message to the controller and staff of the human diving simulation training chamber when the mouse reaches a state of deep anesthesia, so that the staff can enter the human diving simulation training chamber. The controller controls the human diving simulation training chamber to reach the preset environmental parameters, and then the staff removes the deeply anesthetized mouse from the modeling chamber and transfers it to the material collection platform for material collection.

[0012] This invention also provides a method for establishing, anesthetizing, and collecting samples from rats using hyperbaric oxygen poisoning, characterized in that it utilizes the aforementioned integrated system for establishing, anesthetizing, and collecting samples from rats using oxygen poisoning. The method includes:

[0013] Step 1, Experimental Preparation: Check the sealing performance of each chamber, calibrate the accuracy of all sensors, test the linkage function of each valve, breathing anesthesia device and high-definition camera, and import the preset environmental parameters corresponding to the target diving depth of the human diving simulation training chamber into the main controller, including preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range and preset humidity range.

[0014] Step 2, Animal Placement: Place the two rats into the cages in the first and second modeling chambers respectively, and close all valves;

[0015] Step 3: Establishing a High-Pressure Environment: The main controller controls the opening of the main oxygen supply electric valve, the first oxygen supply electric valve, and the second oxygen supply electric valve, and simultaneously starts the first pressure regulating valve and the second pressure regulating valve. High-pressure oxygen from the high-pressure oxygen cylinder flows into the first molding chamber and the second molding chamber respectively, so that the pressure detected by the pressure sensors in the first molding chamber and the second molding chamber reaches the preset pressure range, and the oxygen concentration detected by the oxygen concentration sensor reaches the preset oxygen concentration range, thus forming a high-pressure oxygen environment. At the same time, the small temperature regulator is controlled to start to achieve temperature regulation, so that the temperature detected by the temperature sensors in each molding chamber returns to the preset temperature range. The small humidity regulator is controlled to start to achieve humidity regulation, so that the humidity detected by the humidity sensors in each molding chamber returns to the preset humidity range.

[0016] Step 4, Modeling Monitoring: From the start of modeling, the main controller receives real-time data on the state of the mice in the corresponding modeling chamber recorded by each high-definition camera, analyzes the state of the mice in each modeling chamber, and if a mouse in any modeling chamber exhibits hyperbaric oxygen convulsion, the modeling is completed due to hyperbaric oxygen poisoning, and the corresponding oxygen supply electric valve for that modeling chamber is closed. If mice in two modeling chambers exhibit hyperbaric oxygen convulsion and modeling is completed, all oxygen supply electric valves and the main oxygen supply electric valve are closed.

[0017] Step 5, Anesthesia after Modeling: After the mouse hyperbaric oxygen convulsion model is successfully established in the modeling chamber, the main controller activates the corresponding flow controller to allow the anesthetic in the anesthetic vaporizer to flow into the modeling chamber to administer respiratory anesthesia to the mouse. The controller also monitors the anesthesia concentration fed back by the anesthesia concentration sensor, analyzes the state of the mouse in the modeling chamber, and sends a message to the controller and staff of the human diving simulation training chamber when the mouse reaches a state of deep anesthesia.

[0018] Step Six: Independent Material Preparation: Staff enter the human diving simulation training chamber, and the controller controls the human diving simulation training chamber to reach the preset environmental parameters;

[0019] Step 7, Material Collection: The staff removes the deeply anesthetized mouse from the modeling chamber and transfers it to the material collection table for material collection.

[0020] The positive and progressive effects of this invention are as follows:

[0021] 1. Material collection under high pressure: The dual independent modeling chambers are designed to withstand pressure conditions at a depth of 50 meters. Combined with the human diving simulation training chamber, material collection can be carried out under high pressure after successful mouse modeling, thus preserving the various pathological indicators of mice after oxygen convulsions to the maximum extent and significantly improving the reliability and translational value of the research results.

[0022] 2. Comprehensive monitoring and safety assurance: Real-time tracking of environmental parameters such as pressure, oxygen concentration, and carbon dioxide concentration in each modeling chamber, equipped with high-definition cameras to record the state of rats in the modeling chamber, and automatically triggering corresponding warnings when abnormalities occur, improving the controllability of the experimental process.

[0023] 3. Independent experiments with multiple rats: Two independent modeling chambers allow two rats to be modeled simultaneously, and the environmental parameters of each chamber are independent of each other. When the modeling time of the two rats is different, it can ensure that the material collection process does not affect each other, thus improving experimental efficiency.

[0024] 4. Precise and controllable anesthesia after modeling: Two independent rat respiratory anesthesia devices allow for anesthesia within their respective modeling chambers, simplifying the process. Precise anesthesia is achieved through dual-parameter control of concentration and flow rate, avoiding the transfer risks and depth fluctuations associated with external anesthesia, while also reducing interference from the anesthetic on the modeling environment. While rats often retain consciousness after successful oxygen seizure modeling, immediate decapitation is necessary for brain tissue harvesting. Direct anesthesia within the chamber before decapitation aligns better with animal ethics.

[0025] 5. Optimized equipment space: Placing the material collection workbench directly in the modeling chamber can save experimental space and realize an integrated process of modeling, anesthesia, and material collection. Attached Figure Description

[0026] Figure 1-2 This is a schematic diagram of the structure of the dual independent modeling chambers placed inside the human diving simulation training chamber, which is a preferred embodiment of the present invention.

[0027] Figure 3-7 This is a schematic diagram of the structure of the dual independent molding chambers according to a preferred embodiment of the present invention.

[0028] Figure 8-9 This is a schematic diagram of the hollow cage body according to a preferred embodiment of the present invention.

[0029] Figure 10 This is a schematic diagram of the integrated system for modeling, anesthesia, and sample collection of oxygen poisoning in rats, which is a preferred embodiment of the present invention.

[0030] Figure 11 The flowchart illustrates a preferred embodiment of the method for establishing, anesthetizing, and collecting samples from rats subjected to hyperbaric oxygen poisoning. Detailed Implementation

[0031] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] For ease of description, only the parts relevant to the present invention are shown in the accompanying drawings. The terms "first," "second," etc., used in this invention are merely for the convenience of describing the technical solutions of the invention and do not have a specific limiting effect; they are all general references and do not constitute a limitation on the technical solutions of the present invention. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. Terms indicating positional relationships, such as "middle," "horizontal," "vertical," "longitudinal," "front," "rear," "left," "right," "inner," and "outer," are based on the positional relationships shown in the illustrated drawings and do not imply that the components referred to must be presented in the described positional relationships, and do not constitute a limitation on the technical solutions of the present invention.

[0033] like Figure 1-10 As shown, this embodiment of the invention provides an integrated system for modeling, anesthesia, and material collection of oxygen poisoning in rats. It includes an integrated dual independent modeling chamber placed inside a human diving simulation training chamber 300. The dual independent modeling chamber includes a pressure-resistant chamber 100. A vertical partition 103 is fixed in the middle of the pressure-resistant chamber 100 to divide it into a first modeling chamber 101 and a second modeling chamber 102. Both the first modeling chamber 101 and the second modeling chamber 102 have transparent doors 104 on their front sides. The door of the human diving simulation training chamber 300 is also a transparent door (not shown in the figure). The transparent doors 104 of the first modeling chamber 101 and the second modeling chamber 102 face the transparent door of the human diving simulation training chamber 300. This allows personnel to observe the rats in the first modeling chamber 101 through the transparent doors of the human diving simulation training chamber 300 and the first modeling chamber 101, and to observe the rats in the second modeling chamber 102 through the transparent doors of the human diving simulation training chamber 300 and the second modeling chamber 102. A control box 105 is fixed to the side wall of the first modeling chamber 101 or the second modeling chamber 102. The control box 105 integrates a main controller and is equipped with a touch screen display 106 and an audible and visual alarm 107. The human diving simulation training chamber 300 is existing equipment. The human diving simulation training chamber 300 also has a corresponding controller and display screen. The human diving simulation training chamber 300 is pre-configured with preset environmental parameters corresponding to the target diving depth (such as 50 meters of water depth) stored in the controller. The preset environmental parameters include preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range and preset humidity range.

[0034] like Figure 2As shown, a hollowed-out cage 200 for holding a rat is detachably fixed in both the first molding chamber 101 and the second molding chamber 102. The cage is made of transparent acrylic. Positioning slots 207 are provided at the four corners of the bottom of each cage 200. Four positioning posts 108, corresponding one-to-one with the four positioning slots 207 of the corresponding cage, are fixed to the bottom of both the first molding chamber 101 and the second molding chamber 102. The four positioning slots 207 are respectively inserted into the four positioning posts 108, so that the two cages 200 are respectively fixed in the first molding chamber 101 and the second molding chamber 102.

[0035] The cage 200 specifically includes a perforated cage body 201. A perforated horizontal partition 202 is fixed to the lower part of the cage body 201, dividing it into an upper carrying chamber 203 for holding one rat and a lower collection chamber for collecting waste produced by the rat. A drawer-type collection box 204 is installed in the lower collection chamber. A perforated shell 205 with an open top is added to the outer side wall of the cage body 201. The outer side wall of the cage body 201 and the shell 205 form a carbon dioxide absorption chamber 206 for vertically stacking granular carbon dioxide absorbent. Neither the perforated holes 2011 on the side wall of the cage body 201 nor the perforated holes 2051 on the shell 205 can allow granular carbon dioxide absorbent to pass through. The perforated holes 2011 on the side wall of the cage body 201 correspond to the perforated holes 2051 on the shell 205. The granular carbon dioxide absorbent in the carbon dioxide absorption chamber 206 is used to absorb carbon dioxide in the corresponding modeling chamber.

[0036] In this embodiment, the upper support cavity 203 of the cage 201 is used to support an SD rat, and the drawer-type collection box 204 of the lower collection cavity is used to collect the waste generated by the rat that flows in through the porous structure of the porous horizontal partition 202.

[0037] The first molding chamber 101 and the second molding chamber 102 are respectively connected to one end of the first oxygen supply branch pipe 1 and one end of the second oxygen supply branch pipe 2. The other end of the first oxygen supply branch pipe 1 and the other end of the second oxygen supply branch pipe 2 are both connected to one end of the main oxygen supply pipe 3. The other end of the main oxygen supply pipe 3 is connected to the high-pressure oxygen cylinder 4. The main oxygen supply pipe 3 is equipped with a main oxygen supply electric valve 5. The first oxygen supply branch pipe 1 is equipped with a first oxygen supply electric valve 6 and a first pressure regulating valve 7. The second oxygen supply branch pipe 2 is equipped with a second oxygen supply electric valve 8 and a second pressure regulating valve 9. The first molding chamber 101 and the second molding chamber 102 are each equipped with a small temperature regulator 10, a small humidity regulator 11, a pressure sensor 12, an oxygen concentration sensor 13, a carbon dioxide concentration sensor 14, a temperature sensor 15, a humidity sensor 16, and a high-definition camera 17.

[0038] The rat respiratory anesthesia structure is as follows: the first modeling chamber 101 and the second modeling chamber 102 are each connected to a respiratory anesthesia device. Each respiratory anesthesia device includes an anesthetic vaporizer 18. A flow controller 19 and an anesthetic concentration sensor 20 are sequentially installed on the pipeline between the anesthetic vaporizer 18 and the corresponding modeling chamber.

[0039] In this embodiment, all electric valves, pressure regulating valves, temperature and humidity regulators, flow controllers, and various sensors are existing products.

[0040] Rat sampling structure: A sampling operating table 109 is installed on the top of the dual independent modeling chambers. The sampling operating table 109 is made of stainless steel, and its surface integrates experimental supplies storage slots, dissection fixation table, guillotine, dissection instrument box, and waste collection box.

[0041] In this embodiment, the dual independent modeling chambers are made of pressure-resistant material, capable of withstanding high-pressure conditions (≤0.6MPa) at water depths below 50 meters. Each modeling chamber is equipped with a detachable acrylic cage 200, which features a hollow structure design to ensure uniform gas flow within the chamber. Pressure sensors 12, oxygen concentration sensors 13, carbon dioxide concentration sensors 14, temperature sensors 15, and humidity sensors 16 are embedded in the side walls of each modeling chamber to achieve real-time acquisition of environmental parameters. A pressure regulating valve is connected in series on the connecting pipeline between each modeling chamber and the oxygen supply branch interface to dynamically balance the pressure gradient. Simultaneously, a high-definition camera 17 is installed in each modeling chamber for recording and observing rat behavior.

[0042] The process of establishing a rat hyperbaric oxygen poisoning model, anesthesia, and tissue sampling control in this embodiment is as follows:

[0043] The main controller is used to import the preset environmental parameters corresponding to the target diving depth through the controller of the human diving simulation training chamber 300, or to input the preset environmental parameters corresponding to the target diving depth in advance through the touch screen 106 on the dual independent modeling chambers.

[0044] The main controller is also used to control the opening of the main oxygen supply electric valve 5, the first oxygen supply electric valve 6, and the second oxygen supply electric valve 8, and to simultaneously start the first pressure regulating valve 7 and the second pressure regulating valve 9. High-pressure oxygen from the high-pressure oxygen cylinder 4 flows into the first modeling chamber 101 and the second modeling chamber 102, respectively, so that the pressure detected by the pressure sensor 12 in the first modeling chamber 101 and the second modeling chamber 102 reaches the preset pressure range, and the oxygen concentration detected by the oxygen concentration sensor 13 reaches the preset oxygen concentration range, forming a high-pressure oxygen environment. At the same time, it controls the two small temperature regulators 10 to start to achieve temperature regulation, so that the temperature detected by the temperature sensor 15 in the first modeling chamber 101 and the second modeling chamber 102 returns to the preset temperature range. It also controls the two small humidity regulators 11 to start to achieve humidity regulation, so that the humidity detected by the humidity sensor 16 in the first modeling chamber 101 and the second modeling chamber 102 returns to the preset humidity range.

[0045] The main controller is also used to receive the rat status in the corresponding modeling chamber recorded by each high-definition camera 17 in real time from the start of modeling, analyze the rat status in each modeling chamber, and when a rat in either modeling chamber (first modeling chamber 101 or second modeling chamber 102) exhibits hyperbaric oxygen convulsion, the rat is successfully modeled due to hyperbaric oxygen poisoning, and the corresponding oxygen supply electric valve for that modeling chamber is closed. If rats in both modeling chambers exhibit hyperbaric oxygen convulsion and the model is successfully modeled, all oxygen supply electric valves and the main oxygen supply electric valve 5 are closed.

[0046] For example, if a rat first exhibits hyperbaric oxygen convulsions in the first modeling chamber 101, the rat is successfully modeled for hyperbaric oxygen poisoning, and the first oxygen supply electric valve 6 corresponding to the first modeling chamber 101 is closed. Subsequently, if a rat exhibits hyperbaric oxygen convulsions in the second modeling chamber 102, the rat is successfully modeled for hyperbaric oxygen poisoning, and the second oxygen supply electric valve 8 and the main oxygen supply electric valve 5 corresponding to the second modeling chamber 102 are closed.

[0047] When rats simultaneously exhibit hyperbaric oxygen convulsions in the first modeling chamber 101 and the second modeling chamber 102, the hyperbaric oxygen convulsion model is successfully established in both chambers. In this case, the first oxygen supply electric valve 6, the second oxygen supply electric valve 8, and the main oxygen supply electric valve 5 are closed.

[0048] The main controller is also used to monitor the data detected by each pressure sensor 12, oxygen concentration sensor 13, carbon dioxide concentration sensor 14, temperature sensor 15 and humidity sensor 16 in real time during the molding process, and analyze whether the pressure deviates from the preset pressure range (e.g., the corresponding set value ±0.02MPa), temperature deviates from the preset temperature range (e.g., the corresponding set value ±2℃), humidity deviates from the preset humidity range (e.g., the corresponding set value ±5% RH), oxygen concentration deviates from the preset oxygen concentration range (e.g., the corresponding set value ±1%), or carbon dioxide concentration exceeds the preset carbon dioxide concentration value (e.g., 2%) within a certain period of time in any molding chamber. If so, the controller will control the audible and visual alarm 107 of the molding chamber to issue specific alarm information for that molding chamber.

[0049] The main controller is also used to activate the corresponding flow controller 19 after the rats have successfully undergone hyperbaric oxygen seizure modeling in the modeling chamber. This allows the anesthetic in the anesthetic vaporizer 18 to flow into the modeling chamber to administer respiratory anesthesia to the rats. It also monitors the anesthetic concentration fed back by the anesthetic concentration sensor 20, analyzes the rats' state within the modeling chamber, and sends a deep anesthesia signal to the controller and personnel of the human diving simulation training chamber 300 when the rats reach a deep anesthesia state. This allows personnel to enter the human diving simulation training chamber 300, and the controller maintains the preset environmental parameters within the chamber. The personnel then remove the deeply anesthetized rats from the modeling chamber and transfer them to the sampling platform 109 for sample collection. By first deeply anesthetizing the rats after hyperbaric oxygen seizure modeling, then bringing the same environmental parameters to the human diving simulation training chamber 300, and finally removing the deeply anesthetized rats from the chamber and transferring them to the sampling platform, a sudden change in environmental parameters that could trigger a stress response is avoided, ensuring the stability of the physiological indicators of the samples. In this embodiment, the timing of deep anesthesia of rats and the material collection process are smoothly connected, and rats are directly anesthetized in the modeling chamber after modeling.

[0050] This embodiment includes two independent respiratory anesthesia devices (anesthetic vaporizer, flow controller, and anesthetic concentration sensor), each supplying one of the two modeling chambers. This allows for direct inhalation anesthesia of the mice after successful modeling, avoiding the risk of transfer. The anesthetic concentration sensor monitors the concentration of anesthetic gas within the corresponding modeling chamber and is electrically connected to the main controller. Each modeling chamber has a sealed interface on its side wall for its respective tubing to pass through and connect to the external respiratory anesthesia device. A silicone sealing ring is used at the interface to achieve a pressure seal.

[0051] In this embodiment, an emergency pressure reduction structure is also designed: the first molding chamber 101 and the second molding chamber 102 are respectively connected to one end of the first pressure reduction branch pipe 21 and one end of the second pressure reduction branch pipe 22, and the other end of the first pressure reduction branch pipe 21 and the other end of the second pressure reduction branch pipe 22 are both connected to the pressure reduction main pipe 23. A vacuum pump 24 is installed on the pressure reduction main pipe 23, and a first pressure reduction electric valve 25 and a second pressure reduction electric valve 26 are respectively installed on the first pressure reduction branch pipe 21 and the second pressure reduction branch pipe 22. The main controller is also used to control the vacuum pump 24 to start and the corresponding pressure reduction electric valve of the molding chamber to open when the pressure in the molding chamber exceeds the preset pressure range, so as to realize emergency pressure reduction and bring the pressure detected by the pressure sensor 12 in the molding chamber back to the preset pressure range. When the pressure in the molding chamber is lower than the preset pressure range, the main oxygen supply electric valve 5 and the corresponding oxygen supply electric valve of the molding chamber are controlled to open to realize pressure increase and bring the pressure detected by the pressure sensor in the molding chamber back to the preset pressure range.

[0052] In this embodiment, an emergency exhaust structure is also designed: the first molding chamber 101 and the second molding chamber 102 are respectively connected to a first exhaust pipe 27 and a second exhaust pipe 28, and a first exhaust electric valve 29 and a second exhaust electric valve 30 are respectively installed on the first exhaust pipe 27 and the second exhaust pipe 28; the main controller is also used to control the exhaust electric valve corresponding to the molding chamber to open when the carbon dioxide concentration in the molding chamber exceeds the preset carbon dioxide concentration value, so as to realize the emergency discharge of excess carbon dioxide, and ensure that the carbon dioxide concentration detected by the carbon dioxide concentration sensor 14 in the molding chamber does not exceed the preset carbon dioxide concentration value.

[0053] In this embodiment, the main controller is also used to control the main oxygen supply electric valve 5 and the oxygen supply electric valve corresponding to the molding chamber to open when the pressure in the molding chamber is lower than the preset oxygen concentration range, so as to replenish oxygen and make the oxygen concentration detected by the oxygen concentration sensor 13 in the molding chamber return to the preset oxygen concentration range.

[0054] In this embodiment, the main controller is also used to control the small temperature regulator 10 to start when the temperature in the molding chamber deviates from the preset temperature range, so as to adjust the temperature detected by the temperature sensor 15 in the molding chamber back to the preset temperature range.

[0055] In this embodiment, the main controller is also used to control the small humidity regulator 11 to start when the humidity in the molding chamber deviates from the preset humidity range, so as to adjust the humidity and bring the humidity detected by the humidity sensor 16 in the molding chamber back to the preset humidity range.

[0056] like Figure 11 As shown, this embodiment of the invention also provides a method for establishing, anesthetizing, and collecting samples from rats using hyperbaric oxygen poisoning, which is implemented using the aforementioned integrated system for establishing, anesthetizing, and collecting samples from rats. The method includes:

[0057] Step 1, Experimental Preparation: Check the sealing performance of each chamber, calibrate the accuracy of all sensors, test the linkage function of each valve, breathing anesthesia device and high-definition camera, and import the preset environmental parameters corresponding to the target diving depth of the human diving simulation training chamber 300 into the main controller, including preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range and preset humidity range.

[0058] Step 2, Animal placement: Place the two rats into cages 200 in the first modeling chamber 101 and the second modeling chamber 102 respectively, and close all valves.

[0059] Step 3: Establishing a High-Pressure Environment: The main controller opens the main oxygen supply electric valve 5, the first oxygen supply electric valve 6, and the second oxygen supply electric valve 8, simultaneously activating the first pressure regulating valve 7 and the second pressure regulating valve 9. High-pressure oxygen from the high-pressure oxygen cylinder 4 flows into the first modeling chamber 101 and the second modeling chamber 102, ensuring that the pressure detected by the pressure sensor 12 in both chambers reaches the preset pressure range and the oxygen concentration detected by the oxygen concentration sensor 13 reaches the preset oxygen concentration range, thus forming a high-pressure oxygen environment. Simultaneously, the small temperature regulator 10 is activated to regulate the temperature, ensuring that the temperature detected by the temperature sensor 15 in both chambers reaches the preset temperature range. The small humidity regulator 11 is also activated to regulate the humidity, ensuring that the humidity detected by the humidity sensor 16 in both chambers reaches the preset humidity range.

[0060] Step 4, Modeling Monitoring: From the start of modeling, the main controller receives real-time data from each high-definition camera 17 recording the state of the rats in the corresponding modeling chamber, analyzes the state of the rats in each modeling chamber, and if a rat exhibits hyperbaric oxygen convulsion in either modeling chamber (first modeling chamber 101 or second modeling chamber 102), the hyperbaric oxygen poisoning model is successfully established, and the corresponding oxygen supply electric valve for that modeling chamber is closed. If both rats in the modeling chambers exhibit hyperbaric oxygen convulsion and the model is successfully established, all oxygen supply electric valves and the main oxygen supply electric valve 5 are closed.

[0061] Step 5: Post-modeling respiratory anesthesia: After the mouse hyperbaric oxygen convulsion model is successfully established in the modeling chamber, the main controller activates the corresponding flow controller 19 to allow the anesthetic in the anesthetic vaporizer 18 to flow into the modeling chamber to administer respiratory anesthesia to the mouse. The controller also monitors the anesthetic concentration fed back by the anesthetic concentration sensor 20, analyzes the state of the mouse in the modeling chamber, and sends a message to the controller and staff of the human diving simulation training chamber 300 when the mouse reaches a state of deep anesthesia.

[0062] Step Six: Independent Material Preparation: Staff enter the human diving simulation training chamber 300, and the controller controls the human diving simulation training chamber 300 to reach the preset environmental parameters.

[0063] Step 7, Material Collection: The staff removes the deeply anesthetized mouse from the modeling chamber and transfers it to the material collection operating table 109 for material collection.

[0064] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. An integrated system for inducing oxygen poisoning modeling, anesthesia, and tissue sampling in rats, characterized in that, It includes an integrated dual independent modeling chamber placed inside a human diving simulation training chamber. Each dual independent modeling chamber includes a pressure-resistant chamber body. A vertical partition is fixed in the middle of the pressure-resistant chamber body to divide it into a first modeling chamber and a second modeling chamber. A perforated cage capable of holding a rat is detachably fixed inside both the first and second modeling chambers. The first and second modeling chambers are respectively connected to one end of a first oxygen supply branch pipe and one end of a second oxygen supply branch pipe. The other ends of both the first and second oxygen supply branch pipes are connected to one end of a main oxygen supply pipe. The other end of the main oxygen supply pipe is connected to a high-pressure oxygen supply pipe. The bottles are connected. The main oxygen supply pipe is equipped with a main oxygen supply electric valve. The first oxygen supply branch pipe is equipped with a first oxygen supply electric valve and a first pressure regulating valve. The second oxygen supply branch pipe is equipped with a second oxygen supply electric valve and a second pressure regulating valve. The first and second modeling chambers are each equipped with a small temperature regulator, a small humidity regulator, a pressure sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor, a temperature sensor, a humidity sensor, and a high-definition camera. A control box is fixed to the side wall of the first or second modeling chamber. The control box integrates a main controller and has a display screen and an alarm embedded in it. The first and second modeling chambers are each connected to a respiratory anesthesia device. Each respiratory anesthesia device includes an anesthetic vaporizer. A flow controller and an anesthetic concentration sensor are sequentially installed on the pipeline between the anesthetic vaporizer and the corresponding modeling chamber. A material handling platform is installed on the top of the dual independent molding chambers; The main controller is used to import preset environmental parameters corresponding to the target diving depth of the human diving simulation training chamber, including preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range, and preset humidity range. It controls the main oxygen supply electric valve, the first oxygen supply electric valve, and the second oxygen supply electric valve to open, and simultaneously starts the first pressure regulating valve and the second pressure regulating valve. High-pressure oxygen from the high-pressure oxygen cylinder flows into the first modeling chamber and the second modeling chamber respectively, so that the pressure detected by the pressure sensor in each modeling chamber reaches the preset pressure range, and the oxygen concentration detected by the oxygen concentration sensor reaches the preset oxygen concentration range, forming a high-pressure oxygen environment. At the same time, it controls the small temperature regulator to start to achieve temperature regulation, so that the temperature detected by the temperature sensor in each modeling chamber returns to the preset temperature range, and controls the small humidity regulator to start to achieve humidity regulation, so that the humidity detected by the humidity sensor in each modeling chamber returns to the preset humidity range. The main controller is also used to receive the state of the mice in the corresponding modeling chamber recorded by each high-definition camera in real time from the start of modeling, analyze the state of the mice in each modeling chamber, and when a mouse in any modeling chamber exhibits hyperbaric oxygen convulsion, the modeling is completed due to hyperbaric oxygen poisoning, and the corresponding oxygen supply electric valve of the modeling chamber is closed. If mice in two modeling chambers exhibit hyperbaric oxygen convulsion and modeling is completed, all oxygen supply electric valves and the main oxygen supply electric valve are closed. The main controller is also used to activate the corresponding flow controller after the mouse hyperbaric oxygen convulsion model is successfully established in the modeling chamber, so that the anesthetic in the anesthetic vaporizer flows into the modeling chamber to induce respiratory anesthesia in the mouse, monitor the anesthetic concentration fed back by the anesthetic concentration sensor, analyze the state of the mouse in the modeling chamber, and send a message to the controller and staff of the human diving simulation training chamber when the mouse reaches a state of deep anesthesia, so that the staff can enter the human diving simulation training chamber. The controller controls the human diving simulation training chamber to reach the preset environmental parameters, and then the staff removes the deeply anesthetized mouse from the modeling chamber and transfers it to the material collection platform for material collection.

2. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The main controller is also used to monitor the data detected by each pressure sensor, oxygen concentration sensor, carbon dioxide concentration sensor, temperature sensor and humidity sensor in real time during the molding process, and analyze whether the pressure deviates from the preset pressure range, temperature deviates from the preset temperature range, humidity deviates from the preset humidity range, oxygen concentration deviates from the preset oxygen concentration range, or carbon dioxide concentration exceeds the preset carbon dioxide concentration value in any molding chamber within a certain period of time. If so, it controls the alarm of the molding chamber to issue specific alarm information for that molding chamber.

3. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The first molding chamber and the second molding chamber are respectively connected to one end of the first pressure reducing branch pipe and one end of the second pressure reducing branch pipe. The other end of the first pressure reducing branch pipe and the other end of the second pressure reducing branch pipe are both connected to the pressure reducing main pipe. A vacuum pump is installed on the pressure reducing main pipe. A first pressure reducing electric valve and a second pressure reducing electric valve are respectively installed on the first pressure reducing branch pipe and the second pressure reducing branch pipe. The main controller is also used to control the vacuum pump to start and the pressure reducing electric valve corresponding to the molding chamber to open when the pressure in the molding chamber exceeds the preset pressure range, so as to achieve emergency pressure reduction and bring the pressure detected by the pressure sensor in the molding chamber back to the preset pressure range. When the pressure in the molding chamber is lower than the preset pressure range, the controller controls the main oxygen supply electric valve and the oxygen supply electric valve corresponding to the molding chamber to open, so as to achieve pressurization and bring the pressure detected by the pressure sensor in the molding chamber back to the preset pressure range.

4. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The first molding chamber and the second molding chamber are respectively connected to a first discharge pipe and a second discharge pipe, and a first discharge electric valve and a second discharge electric valve are respectively installed on the first discharge pipe and the second discharge pipe. The main controller is also used to control the corresponding electric discharge valve of the molding chamber to open when the carbon dioxide concentration in the molding chamber exceeds the preset carbon dioxide concentration value, so as to discharge the excess carbon dioxide in an emergency and ensure that the carbon dioxide concentration detected by the carbon dioxide concentration sensor in the molding chamber does not exceed the preset carbon dioxide concentration value. The main controller is also used to control the main oxygen supply electric valve and the corresponding oxygen supply electric valve of the molding chamber to open when the pressure in the molding chamber is lower than the preset oxygen concentration range, so as to replenish oxygen and make the oxygen concentration detected by the oxygen concentration sensor in the molding chamber return to the preset oxygen concentration range.

5. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The main controller is also used to control a small temperature regulator to start when the temperature in the molding chamber deviates from the preset temperature range, so as to adjust the temperature detected by the temperature sensor in the molding chamber back to the preset temperature range. The main controller is also used to control a small humidity regulator to start when the humidity in the molding chamber deviates from the preset humidity range, so that the humidity detected by the humidity sensor in the molding chamber returns to the preset humidity range.

6. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The first and second modeling chambers are each equipped with a transparent door on their front sides. The door of the human diving simulation training chamber is also a transparent door. The transparent doors of the first and second modeling chambers face the transparent door of the human diving simulation training chamber.

7. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, The cage includes a perforated cage body, with a perforated horizontal partition fixed in the lower part of the cage body to divide the cage body into an upper carrying chamber for carrying a rat and a lower collection chamber for collecting the waste produced by the rat. A drawer-type collection box is provided in the lower collection chamber.

8. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 7, characterized in that, The cage has an additional open-top perforated shell on the outer side wall. The outer side wall of the cage and the shell form a carbon dioxide absorption chamber for vertically stacking granular carbon dioxide absorbent. The perforated holes on the side wall of the cage and the perforated holes on the shell cannot allow granular carbon dioxide absorbent to pass through. The perforated holes on the side wall of the cage correspond to the perforated holes on the shell.

9. The integrated system for establishing, anesthetizing, and harvesting oxygen poisoning models in rats as described in claim 1, characterized in that, Positioning slots are provided at the four corners of the bottom of both cages. The bottom of the first molding chamber and the second molding chamber are fixed with four positioning posts that correspond one-to-one with the four positioning slots of the corresponding cages. The four positioning slots are respectively inserted into the four positioning posts so that the two cages are respectively fixed in the first molding chamber and the second molding chamber.

10. A method for inducing, anesthetizing, and collecting samples from rats using hyperbaric oxygen poisoning, characterized in that, It is achieved using the integrated system for rat oxygen poisoning modeling, anesthesia, and tissue sampling as described in any one of claims 1-9, the method comprising: Step 1, Experimental Preparation: Check the sealing performance of each chamber, calibrate the accuracy of all sensors, test the linkage function of each valve, breathing anesthesia device and high-definition camera, and import the preset environmental parameters corresponding to the target diving depth of the human diving simulation training chamber into the main controller, including preset pressure range, preset oxygen concentration range, preset carbon dioxide concentration value, preset temperature range and preset humidity range. Step 2, Animal Placement: Place the two rats into the cages in the first and second modeling chambers respectively, and close all valves; Step 3: Establishing a High-Pressure Environment: The main controller controls the opening of the main oxygen supply electric valve, the first oxygen supply electric valve, and the second oxygen supply electric valve, and simultaneously starts the first pressure regulating valve and the second pressure regulating valve. High-pressure oxygen from the high-pressure oxygen cylinder flows into the first molding chamber and the second molding chamber respectively, so that the pressure detected by the pressure sensors in the first molding chamber and the second molding chamber reaches the preset pressure range, and the oxygen concentration detected by the oxygen concentration sensor reaches the preset oxygen concentration range, thus forming a high-pressure oxygen environment. At the same time, the small temperature regulator is controlled to start to achieve temperature regulation, so that the temperature detected by the temperature sensors in each molding chamber returns to the preset temperature range. The small humidity regulator is controlled to start to achieve humidity regulation, so that the humidity detected by the humidity sensors in each molding chamber returns to the preset humidity range. Step 4, Modeling Monitoring: From the start of modeling, the main controller receives real-time data on the state of the mice in the corresponding modeling chamber recorded by each high-definition camera, analyzes the state of the mice in each modeling chamber, and if a mouse in any modeling chamber exhibits hyperbaric oxygen convulsion, the modeling is completed due to hyperbaric oxygen poisoning, and the corresponding oxygen supply electric valve for that modeling chamber is closed. If mice in two modeling chambers exhibit hyperbaric oxygen convulsion and modeling is completed, all oxygen supply electric valves and the main oxygen supply electric valve are closed. Step 5, Anesthesia after Modeling: After the mouse hyperbaric oxygen convulsion model is successfully established in the modeling chamber, the main controller activates the corresponding flow controller to allow the anesthetic in the anesthetic vaporizer to flow into the modeling chamber to administer respiratory anesthesia to the mouse. The controller also monitors the anesthesia concentration fed back by the anesthesia concentration sensor, analyzes the state of the mouse in the modeling chamber, and sends a message to the controller and staff of the human diving simulation training chamber when the mouse reaches a state of deep anesthesia. Step Six: Independent Material Preparation: Staff enter the human diving simulation training chamber, and the controller controls the human diving simulation training chamber to reach the preset environmental parameters; Step 7, Material Collection: The staff removes the deeply anesthetized mouse from the modeling chamber and transfers it to the material collection table for material collection.

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