Freeze tube with sealing cap

Abstract

The application provides a cryopreservation tube with a sealing cover, comprising a tube body, a plug body and a fixing member. The tube body has a cavity, an opening, first clamping teeth, second clamping teeth and a convex tooth part. The first clamping teeth and the second clamping teeth are arranged on the peripheral surface of one end of the tube body. The fixing member is tubular, arranged on one end of the tube body to fix the plug body in the opening, and has a first groove, a second groove, a third groove, a positioning part, a cover and a concave tooth part. The first groove and the second groove are located on the inner circumferential surface of the fixing member. In use, the plug body can be first arranged in the opening, and then the bottom opening of the fixing member is pressed towards the opening to make the inner circumferential surface of the fixing member slide along the peripheral surface of the tube body towards the other end of the tube body. Until the second groove slides through the first clamping teeth, and then the second clamping teeth are clamped with each other. In addition, a breakable part is arranged between the cover and the fixing member, so that the cover can be twisted off from the fixing member to expose the plug body.

Description

[0001] This application claims priority in a patent application filed on December 5, 2024, with application number 113147323, in Taiwan. Technical Field

[0002] This invention relates to the field of tube structures, and in particular to a cryopreservation tube for preserving cells or other biologically related materials. Background Technology

[0003] Biomedical research typically begins with cell experiments. Only after preliminary research results are obtained from cell experiments will animal experiments and clinical trials follow. Therefore, in vitro cultured cell lines are an indispensable research material in biomedical experiments, and cell preservation is of paramount importance.

[0004] In addition, cell therapy is an emerging treatment applied to cancer treatment and regenerative medicine. In cancer treatment, the number of a patient's immune cells can be increased by culturing cells in vitro, and then these immune cells are injected into the patient's body, ultimately relying on the increased immune cells to kill cancer cells. In regenerative medicine, the differentiation and proliferation properties of stem cells are utilized to repair a patient's organs or tissues. Because cell therapy involves injecting cells into the patient's body, the preservation of these cells requires even greater care.

[0005] Liquid nitrogen cryopreservation is currently the most commonly used cell cryopreservation technique, which involves placing healthy cells into cryovials and storing them in a liquid nitrogen tank.

[0006] It is important to note that the process of loading cells into cryovials or removing them from cryovials is when cells are most susceptible to contamination, and sterility must be maintained throughout. Therefore, users typically operate in a biosafety cabinet, and the process must be extremely careful to prevent microbial contamination of the cells.

[0007] Known cryovials consist of a tube body and a cap. The cap is typically fitted tightly into the opening of the tube body to ensure a complete seal of the tube's cavity and prevent cell contamination by microorganisms. Therefore, removing the cap from the tube body when loading or removing cells is quite difficult. Furthermore, after removing the cap, the tube's cavity is directly exposed to the external space. While this facilitates cell loading or removal, it also makes the cells more susceptible to microbial contamination.

[0008] Another known cryovial has been developed, comprising a tube body, a stopper, and a screw cap. During cell loading, the frozen culture medium containing the cells is first loaded into the chamber of the tube body. Then, the stopper seals the opening of the tube body, and finally, the screw cap secures the stopper to the opening. During cell retrieval, the screw cap is removed to expose the stopper. A needle is then inserted through the stopper into the chamber of the tube body to retrieve the cells. The stopper ensures that the chamber of the tube remains sealed and does not communicate with the outside environment, preventing microbial contamination of the cells. However, while this new cryovial solves the problem of susceptibility to microbial contamination, it introduces new problems during the assembly stage.

[0009] Most cryopreservation tube fixation caps utilize threaded cap structures. Threaded caps can be further divided into internal screw caps (with threads inside the tube) and external screw caps (with threads on the outside). Generally, internal screw caps offer a better seal than external screw caps because they directly plug the tube opening and can lock more tightly. External screw caps, on the other hand, have the advantage of having their bottom end outside the tube, preventing contact with cells as with internal screw caps. This effectively avoids microbial contamination and cell contact when the cap is placed on a table. Furthermore, regardless of the type, both types of caps must have a certain gap in their structure to allow for rotation, which could lead to seal failure.

[0010] Reducing the gap would increase the difficulty of manually locking the cap, potentially causing finger injuries to assemblers. Using a mechanical cap requires additional securing of the tube to prevent it from spinning freely, but this could easily damage the tube.

[0011] Furthermore, it is known that cryopreservation tubes are usually sealed with O-rings, which results in a less than ideal seal. If the tube opening is completely sealed with a cork, it may be difficult to remove for reuse, and it is also possible that the tube may fall into the body due to shrinkage during freezing.

[0012] Furthermore, before handling cells during transport or in a biosafety cabinet, users can determine whether the cell seal has been opened by checking the condition of the sealing sticker. If the sticker is intact, the seal is unopened and the cells are uncontaminated. If the sticker is damaged, the seal has been opened, and there is a risk of cell contamination. Users must manually remove the sticker before handling the cells. Clearly, while the sealing sticker serves as a preventative measure against cell contamination, it increases the user's workload, creating a dilemma for them.

[0013] In view of the shortcomings of known cryopreservation tubes, such as: insufficient sealing of screw-cap structure and cumbersome assembly conditions; easy contamination when the inner screw cap is placed on the table, and poor sealing effect of the outer screw cap; difficulty in achieving both sealing and easy removal of the stopper; and the increased operational burden due to the precautions to ensure that cells are not contaminated, the inventors of this case have been actively and continuously developing inventions that can solve the above problems. Summary of the Invention

[0014] In view of the aforementioned problems, the main objective of this invention is to provide a cryopreservation tube with a sealing cap, wherein the fixing component connects the tube body and fixes the plug body simultaneously through the sealing structure, replacing the screw cap fixing structure of known cryopreservation tubes, reducing assembly time and conditions, and thus accelerating the time for products to enter cryopreservation.

[0015] Another objective of this invention is to provide a cryopreservation tube with a sealing cap, which increases the connection strength between the plug and the fixing member by means of a raised structure, and uses a flange structure to prevent the plug from getting stuck in the tube body and being difficult to remove.

[0016] To achieve the aforementioned objectives, the present invention provides a cryopreservation tube with a sealing cap, comprising: a tube body, a plug, and a fixing member. One end of the tube body is a first end, and the opposite end is a second end. The tube body has: a chamber, an opening, a first locking tooth, a second locking tooth, and a protruding tooth. The chamber is formed inside the tube body; the opening is located at the first end connecting the chamber to the outside; the first locking tooth and the second locking tooth are located on the outer surface of the first end, with the second locking tooth being closer to the second end than the first locking tooth. The protruding tooth is located on the outer surface of the first end, closer to the opening than the first locking tooth. The outer surface of the tube body, from the first end to the second end, consists of the protruding tooth, the first locking tooth, and the second locking tooth in sequence. The plug is located at the first end of the tube body to seal the opening, and the axis of the plug is aligned with the axis of the tube body.

[0017] The fixing member is tubular and disposed at the first end, used to fix the plug to the first end; one end of the fixing member is the top end, and the other end is the bottom end. The inner diameter of the top end side of the fixing member is recessed to form a step, and it has: a first groove, a second groove, a third groove, a positioning part, a cap, and a toothed part. The first groove and the second groove are located on the inner circumferential surface of the bottom end of the fixing member, and the second groove is closer to the bottom end of the fixing member than the first groove; the third groove is located on the inner circumferential surface of the top end side of the fixing member where the inner diameter is recessed; the positioning part is the step plane of the fixing member.

[0018] The cap is disposed at the top of the fastener to close the opening; the concave tooth portion is disposed on the inner circumferential surface of the fastener, between the step and the first groove. The inner circumferential surface of the fastener, from the top to the bottom, consists of a third groove, a positioning portion, a concave tooth portion, a first groove, and a second groove in sequence.

[0019] In a preferred embodiment of the present invention, the first groove and the first locking tooth, and the second groove and the second locking tooth, are all shapes that can be correspondingly engaged with each other for combining the fixing member and the tube body. Simultaneously, the plug body is fixed at the first end to close the opening. The cap can be opened to expose the plug body, allowing the user to pierce the plug body with a needle to enter the chamber and extract frozen culture medium containing cells.

[0020] In detail, the plug can be first placed in the opening, and then pressed against the first end with the bottom opening of the fastener, causing the inner surface of the fastener to slide along the outer surface of the tube towards the second end. This continues until the second groove slides past the first and second locking teeth, which engage with each other. Simultaneously, the convex and concave teeth, as well as the first groove and the first locking tooth, also engage with each other. The convex and concave teeth prevent the fastener from rotating about the axis of the tube; the engagement of the first locking tooth with the first groove and the second locking tooth with the second groove ensures a secure connection between the fastener and the tube.

[0021] In a preferred embodiment of the present invention, the second locking tooth and the second groove together form a backstop structure to prevent the fastener from being easily removed from the tube body.

[0022] In a preferred embodiment of the present invention, the plug body has a top end and a bottom end, and includes an assembly protrusion, a flange, a puncture portion, a guide portion, a sealing portion, and a recess. The assembly protrusion is located on the outer peripheral surface of the top end side of the plug body; the flange protrudes from the outer peripheral surface of the top end side of the plug body and is closer to the bottom end than the assembly protrusion.

[0023] In a preferred embodiment of the present invention, the assembly protrusion corresponds to the third groove, and the outer diameter of the top end of the plug can be inserted into the inner diameter of the top end of the fixing member, so that the assembly protrusion and the third groove can engage with each other to connect the fixing member and the plug.

[0024] In a preferred embodiment of the present invention, when the fixing member is pressed onto the tube body to fix the plug, the flange abuts against the end face of the first end of the tube body on one side near the bottom end of the plug body, and against the positioning part of the fixing member on the other side. The flange can increase the sealing effect of the plug body, effectively increasing the sealing degree when the fixing member is pressed tightly. The flange can also prevent the plug body from being pressed into the cavity of the tube body by the fixing member by abutting against the end face of the first end of the tube body on one side, thus preventing the plug body from being stuck in the cavity.

[0025] In a preferred embodiment of the present invention, the puncture portion is disposed at the top end of the plug and is covered by a cap. The guide portion is disposed at the bottom end of the plug and is located inside the cavity when the plug and the tube are assembled. The outer diameter of the guide portion tapers towards the bottom end of the plug to facilitate insertion into the cavity. The sealing portion is disposed on the outer surface of the plug between the puncture portion and the guide portion, and is in close contact with the inner surface of the tube to seal the opening. The recessed portion extends sequentially from the bottom end of the plug through the interior of the guide portion and the interior of the sealing portion, and extends into the interior of the puncture portion but does not penetrate the puncture portion to form a blind hole.

[0026] Preferably, the inner diameter of the recess gradually increases from the inside of the puncture portion toward the bottom of the plug.

[0027] Preferably, the axis of the recess is aligned with the plug body.

[0028] In a preferred embodiment of the present invention, the thickness of the flange is 1.0 to 1.4 mm, preferably 1.2 mm.

[0029] Preferably, the plug is a polymeric elastic material.

[0030] In a preferred embodiment of the present invention, the cap is connected to the fixing member by a connecting portion and is provided with a breakable portion, the smallest diameter of which is defined as the breakable portion. Since the diameter of the breakable portion is smaller than the diameter of the rest of the connecting portion, the breakable portion will break due to stress concentration when the cap is rotated. Attached Figure Description

[0031] Figure 1 This is a three-dimensional schematic diagram of an embodiment of the present invention;

[0032] Figure 2 This is a side view of the tube body according to an embodiment of the present invention;

[0033] Figure 3 for Figure 1 A cross-sectional view of the fastener in the AA direction of an embodiment of the present invention;

[0034] Figure 4 for Figure 1 A cross-sectional view of the plug body in the AA direction of an embodiment of the present invention;

[0035] Figure 5 for Figure 1 A first assembly cross-sectional view in the AA direction of an embodiment of the present invention;

[0036] Figure 6 for Figure 1 A second assembly cross-sectional view in the AA direction of an embodiment of the present invention;

[0037] Figure 7 for Figure 1A third assembly cross-sectional view in the AA direction of an embodiment of the present invention;

[0038] Figure 8 This is a schematic diagram of the first application of an embodiment of the present invention.

[0039] Symbol explanation:

[0040] 10-tube body;

[0041] 101 - First end;

[0042] 102 - Second end;

[0043] 11-chamber;

[0044] 12-Opening;

[0045] 13-First locking tooth;

[0046] 14-Second locking tooth;

[0047] 15-convex toothed portion;

[0048] 20-Plug;

[0049] 21- Assemble the protrusion;

[0050] 22-Flange;

[0051] 23-Puncture site;

[0052] 24-Guiding section;

[0053] 25 - Sealing part;

[0054] 26 - Depression;

[0055] 30 - Fastener;

[0056] 31-First trench;

[0057] 32-Second trench;

[0058] 33 - Third trench;

[0059] 34 - Positioning section;

[0060] 341-Difference;

[0061] 35 - Cap;

[0062] 351 - Connecting part;

[0063] 352 - Easily broken section;

[0064] 36 - Concave tooth section. Detailed Implementation

[0065] To facilitate understanding of the present invention, a detailed description is provided below with reference to the accompanying drawings and embodiments. The drawings show some, but not all, embodiments of the invention. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without any inventive effort are within the scope of protection of the present invention.

[0066] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

[0067] Figures 1 to 7 This is a schematic diagram of an embodiment of the present invention, such as... Figure 1 As shown in the perspective schematic diagram of an embodiment of the present invention, the cryopreservation tube with a sealing cap of the present invention includes: a tube body 10, a plug body 20, and a fixing member 30. For example... Figure 2 A cross-sectional view of the tube body 10 according to an embodiment of the present invention is shown. One end of the tube body 10 is a first end 101, and the other end is a second end 102. The tube body 10 has: a chamber 11, an opening 12, a first locking tooth 13, a second locking tooth 14, and a protruding tooth portion 15. The chamber 11 is formed inside the tube body 10; the opening 12 is disposed at the first end 101, connecting the chamber 11 to the outside; the first locking tooth 13 and the second locking tooth 14 are disposed on the outer peripheral surface of the first end 101, with the second locking tooth 14 being closer to the second end 102 than the first locking tooth 13. The protruding tooth portion 15 is disposed on the outer peripheral surface of the opening 12. The outer peripheral surface of the tube body 10, from the first end 101 to the second end 102, consists sequentially of the protruding tooth portion 15, the first locking tooth 13, and the second locking tooth 14. The plug 20 is disposed at the first end 101 of the tube 10 to close the opening 12, and the axis of the plug 20 is aligned with the axis of the tube 10.

[0068] like Figure 3The cross-sectional view of the fixing member 30 in an embodiment of the present invention is shown. The fixing member 30 is tubular and disposed at the first end 101, used to fix the plug 20 to the first end 101 to seal the opening 12. One end of the fixing member 30 is the top end, and the other end is the bottom end. The inner diameter of the top end side of the fixing member 30 is reduced to form a step 341, and it has: a first groove 31, a second groove 32, a third groove 33, a positioning part 34, a cap 35, and a toothed part 36. The first groove 31 and the second groove 32 are located on the inner circumferential surface of the bottom end of the fixing member 30, and the second groove 32 is closer to the bottom end of the fixing member 30 than the first groove 31; the third groove 33 is located on the inner circumferential surface of the top end side of the fixing member 30 after the inner diameter is reduced; the positioning part 34 is the plane of the step 341 of the fixing member 30.

[0069] The cap 35 is disposed at the top of the fastener 30 to close the opening at the top of the fastener 30; the concave tooth portion 36 is disposed on the inner circumferential surface of the fastener 30, between the step 341 and the first groove 31. The inner circumferential surface of the fastener 30, from the top to the bottom, consists of the third groove 33, the positioning portion 34, the concave tooth portion 36, the first groove 31, and the second groove 32 in sequence.

[0070] In a preferred application, the first groove 31 and the first locking tooth 13, as well as the second groove 32 and the second locking tooth 14, are all shaped to engage with each other, for mounting the fixing member 30 to the tube body 10. When the fixing member 30 is assembled with the tube body 10, the fixing member 30 also simultaneously fixes the plug body 20 to the first end 101 to seal the opening 12. The user can open the cap 35 to expose the plug body 20 and pierce the plug body 20 with a needle to enter the chamber 11 to extract culture medium containing cells.

[0071] like Figure 4 As shown in the cross-sectional view of the plug body 20 in an embodiment of the present invention, in a preferred embodiment of the present invention, one end of the plug body 20 is a top end, and the other end is a bottom end, and it has: an assembly protrusion 21, a flange 22, a puncture portion 23, a guide portion 24, a sealing portion 25, and a recessed portion 26. The assembly protrusion 21 is located on the peripheral surface of the top end side of the plug body 20; the flange 22 protrudes from the peripheral surface of the top end side of the plug body 20 and is closer to the bottom end than the assembly protrusion 21.

[0072] The puncture portion 23 is located at the top of the plug body 20, and when the plug body 20 is fixed by the fixing member 30, the puncture portion 23 is covered by the cap 35. The guide portion 24 is located at the bottom of the plug body 20. When the plug body 20 is installed to the first end 101, the guide portion 24 is located inside the chamber 11. The outer diameter of the guide portion 24 tapers towards the bottom of the plug body 20 to facilitate insertion into the chamber 11. The sealing portion 25 is located on the outer surface of the plug body 20 between the puncture portion 23 and the guide portion 24. The sealing portion 25 is in close contact with the inner surface of the tube body 10 to seal the chamber 11. The recessed portion 26 sequentially penetrates the interior of the guide portion 24 and the interior of the sealing portion 25 from the bottom of the plug body 20, and extends into the interior of the puncture portion 23 but does not penetrate the puncture portion 23 to form a blind hole.

[0073] Preferably, the inner diameter of the recess 26 gradually increases from the inside of the puncture portion 23 toward the bottom of the plug 20.

[0074] Preferably, the axis of the recess 26 is aligned with the plug 20.

[0075] like Figure 5 As shown in the first assembly cross-sectional view of the embodiment of the present invention, the plug 20 is first installed on the first end 101 of the tube 10, and the bottom end of the plug 20 is inserted into the chamber 11 by the guide part 24; the sealing part 25 is in contact with the inner circumferential surface of the tube 10 to seal the opening 12; at the same time, the side of the flange 22 near the bottom end of the plug 20 abuts against the end face of the first end 101.

[0076] like Figure 6 As shown in the second assembly cross-sectional view of the embodiment of the present invention, the bottom opening of the fixing member 30 is pressed onto the first end 101, so that the bottom opening of the fixing member 30 slides along the outer surface of the tube body 10 toward the second end 102. This continues until the positioning part 34 abuts against the side of the flange 22 near the top of the plug body 20; simultaneously, the first groove 31 engages with the first locking tooth 13 and the second groove 32 engages with the second locking tooth 14, completing the assembly of the fixing member 30 and the tube body 10.

[0077] Specifically, the flange 22 can enhance the sealing effect of the plug 20 and prevent the plug 20 from being pressed into the chamber 11 by the fixing member 30. When the positioning part 34 presses against the tube 10, the flange 22 acts as a buffer between the fixing member 30 and the tube 10, and the sealing effect is enhanced due to the tightness of the pressing of the tube 10 by the fixing member 30. At the same time, because one side of the flange 22 abuts against the end face of the first end 101, the plug 20 is less likely to be pressed into the chamber 11 during the pressing process.

[0078] like Figure 6As shown, the second groove 32 and the second locking tooth 14 together form a backstop structure. One side of the second groove 32 near the bottom of the fixing member 30 is a vertical side, which is perpendicular to the outer surface of the tube body 10 in the radial direction along the axis of the tube body 10; the side of the second locking tooth 14 near the second end 102 corresponds to the vertical side of the second groove 32 to achieve backstop of the fixing member 30.

[0079] In a preferred embodiment of the present invention, the thickness of the flange 22 is 1.0 to 1.4 mm, preferably 1.2 mm.

[0080] A preferred embodiment of the present invention, such as Figure 6 As shown, when the fastener 30 and the tube body 10 are assembled, the assembly protrusion 21 also engages with the third groove 33 to connect the fastener 30 and the plug 20. The plug 20 can be removed along with the fastener 30.

[0081] like Figure 7 The third assembly cross-sectional view of the present invention shows that the fixing member 30 and the plug body 20 are assembled first by assembling the protrusion 21 and the third groove 33. When assembling cryopreservation tubes with sealing caps on the production line, the fixing member 30 and the plug body 20 can be directly installed onto the tube body 10 by manual labor or a robotic arm, without the need for multiple assembly steps to increase assembly efficiency.

[0082] like Figure 8 As shown in the first application diagram of the present invention, the cover 35 is connected to the fixing member 30 by a connecting portion 351 and is provided with a breakable portion 352. The smallest diameter of each connecting portion 351 is defined as the breakable portion 352. Because the diameter of the breakable portion 352 is smaller than the diameter of the rest of each connecting portion 351, the user only needs to apply a little force to rotate the cover 35, and the breakable portion 352 will be twisted off due to stress concentration.

[0083] Specifically, if the connector 351 remains connected to the retainer 30 during transport or before the cells are retrieved from the biosafety cabinet, it indicates that the retainer 30 has not been opened and the cells are not contaminated. If the connector 351 has been broken and the cap 35 has been removed during transport or before the cells are retrieved from the biosafety cabinet, it indicates that the retainer 30 has been opened and there is a risk of cell contamination.

[0084] The embodiments described above are merely preferred embodiments of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make various changes and modifications without departing from the concept of the present invention, and these all fall within the scope of protection of the present invention.

Claims

1. A cryopreservation tube with a sealing cap, characterized in that, include: A tube body has a chamber, an opening and a first locking tooth. The opening is located at a first end of the tube body and connects the chamber to the outside. The first locking tooth is located on the outer surface of the first end. A plug is disposed at the first end to seal the opening; as well as A fixing member, disposed at the first end and being tubular, is used to fix the plug body to the first end. The fixing member has a first groove and a cap, the cap closing one end opening of the fixing member, and the first groove being located on the inner circumferential surface of the other end opening of the fixing member. The first groove and the first locking tooth are in a corresponding and engaging shape to combine the fixing member and the tube body, and the cap can be opened to expose the plug.

2. The cryopreservation tube with a sealing cap according to claim 1, characterized in that, The tube body also has a second locking tooth located on the outer peripheral surface of the first end, and the fixing member also has a second groove located on the inner peripheral surface of the opening away from the sealing side. The second groove and the second locking tooth are in a mutually engaging shape, and the second groove and the second locking tooth together form a backstop structure.

3. The cryopreservation tube with a sealing cap according to claim 1, characterized in that, The outer surface of the first end is also provided with a protruding tooth, and the inner circumferential surface of the fastener near the sealing side is also provided with a concave tooth corresponding to the protruding tooth.

4. The cryopreservation tube with a sealing cap according to claim 1, characterized in that, The inner surface of the fastener near the end of the cap also has a third groove, and the plug has an assembly protrusion corresponding to the third groove for assembling the plug and the fastener.

5. The cryopreservation tube with a sealing cap according to claim 1, characterized in that, The plug has a flange, and the first groove of the fastener and the cap also have a positioning part, which is formed as a step that reduces the inner diameter of the fastener toward the cap side; The flange abuts against the end face of the first end on one side near the bottom end of the plug, and against the positioning part on the other side.

6. The cryopreservation tube with a sealing cap according to any one of claims 1 to 5, characterized in that, The fastener also has a connecting portion and a breakable portion, the cover is connected to the fastener by the connecting portion, and the breakable portion is defined at the smallest point of the diameter of the connecting portion.

7. The cryopreservation tube with a sealing cap according to claim 6, characterized in that, The axis of the plug is aligned with the axis of the tube, and it also has: A puncture site is located at the top of the plug and is covered by the cap; A guide portion is disposed at the bottom end of the plug body, located within the chamber, and the outer diameter of the guide portion tapers towards the bottom end; and A sealing part is disposed on the outer surface of the plug body between the puncture part and the guide part, and contacts the inner surface of the tube body to seal the opening.

8. The cryopreservation tube with a sealing cap according to claim 7, characterized in that, The plug also has a recessed portion that sequentially penetrates the interior of the guide portion and the interior of the sealing portion from the bottom end of the plug, and extends to the interior of the puncture portion.

9. The cryopreservation tube with a sealing cap according to claim 8, characterized in that, The inner diameter of the recess gradually increases from the inside of the puncture portion toward the bottom.

10. The cryopreservation tube with a sealing cap according to claim 9, characterized in that, The axis of the recess is aligned with the axis of the plug.

11. The cryopreservation tube with a sealing cap according to claim 5, characterized in that, The thickness of the flange is 1.0 to 1.4 mm.

12. The cryopreservation tube with a sealing cap according to claim 1, characterized in that, The plug is made of a high-molecular elastic material.

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