A suspension containing prothioconazole, pyraclostrobin and glycine and a method for preparing the same

Abstract

The application relates to the technical field of pesticide compound preparations, and particularly relates to a suspension agent containing prothioconazole, pyraclostrobin and glycine and a preparation method thereof; and a pesticide composition, which comprises active ingredient A, active ingredient B and active ingredient C; the active ingredient A is prothioconazole; the active ingredient B is pyraclostrobin; and the active ingredient C is glycine; the mass concentration of the prothioconazole is 130-170 g / L; the mass concentration of the pyraclostrobin is 130-170 g / L; and the mass concentration of the glycine is 130-170 g / L; the prothioconazole, the pyraclostrobin and the glycine are compounded in the application to obtain a composite fungicide; the prothioconazole, the pyraclostrobin and the glycine play a complementary and synergistic role, show excellent inhibition and killing effects on wheat scab fungi, wheat stem base rot fungi fusarium graminearum and other disease fungi, the wheat is not damaged by the disease fungi in the growth process, and thus shows a good growth trend and the yield is also increased.

Description

Technical Field

[0001] This application relates to the technical field of pesticide compound formulations, and in particular to a suspension containing prothioconazole, pyraclostrobin and glycine and its preparation method. Background Technology

[0002] Fungal diseases in crops are a key factor restricting high-quality and high-yield agricultural production. With the development of intensive planting patterns, the frequency and severity of disease occurrence have increased, and the problem of fungal resistance is becoming increasingly prominent, placing higher demands on the control efficacy and sustainability of fungicides. Prothioconazole, a highly effective triazole thionate fungicide, has excellent control efficacy against various fungal diseases such as wheat sheath blight and corn leaf blight due to its strong systemic activity and broad spectrum of action. Pyraclostrobin, a methoxyacrylate fungicide, works by inhibiting the respiratory chain of pathogens, possessing protective, curative, and eradicative activities, and showing significant control effects against diseases such as downy mildew and anthracnose. However, when these two fungicides are used alone, pathogens easily develop resistance rapidly, leading to decreased efficacy and increased dosage. Furthermore, both fungicides have limited residual effects and insufficient synergistic effects on crop growth.

[0003] Glycine, as a natural amino acid, can not only enhance crop resistance and promote growth and development, but also improve the efficacy of fungicides by enhancing their adsorption and conduction efficiency on crop surfaces. However, when prothioconazole, pyraclostrobin and glycine are mixed, compatibility problems such as layering, precipitation and crystallization are easily caused by excessive differences in interfacial tension, making it difficult to form a stable formulation system.

[0004] Therefore, how to effectively solve the problems of poor compatibility and poor stability of formulations has become an urgent issue to be addressed. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this application provides a suspension containing prothioconazole, pyraclostrobin, and glycine, and a method for preparing the same.

[0006] In a first aspect, this application provides a pesticide composition, which adopts the following technical solution: A pesticide composition comprising active ingredient A, active ingredient B, and active ingredient C, wherein active ingredient A is prothioconazole; active ingredient B is pyraclostrobin; and active ingredient C is glycine.

[0007] Preferably, the mass concentration of prothioconazole is 130-170 g / L; the mass concentration of pyraclostrobin is 130-170 g / L; and the mass concentration of glycine is 130-170 g / L.

[0008] By adopting the above technical solution, the three active ingredients in this application have different mechanisms of action, and the combination achieves an unexpected synergistic effect. The combination of the three active ingredients shows good bactericidal performance and enhances crop immunity.

[0009] Secondly, this application provides a suspending agent, which adopts the following technical solution: A suspending agent comprising the following raw materials: A pesticide composition comprising 130-170 g / L of prothioconazole, 130-170 g / L of pyraclostrobin, and 130-170 g / L of glycine.

[0010] Coating agent 30-50 g / L; Emulsifier 30-50g / L; Dispersant 20-30 g / L; Thickener 1-5g / L; Defoamer 1-3g / L; Antifreeze 5-10g / L.

[0011] Preferably, the coating agent comprises the following raw materials in parts by weight: 0.5-1.2 parts of amino gum, 1-3 parts of attapulgite, 2-6 parts of γ-aminopropyltrimethoxysilane, 0.2-0.4 parts of hydroquinone, 1-3 parts of attapulgite, 2-6 parts of 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride, 0.2-0.4 parts of hydroquinone, 0.5-1.5 parts of acrylamide, 0.01-0.05 parts of ammonium persulfate, 10-15 parts of chitosan, 10-15 parts of glacial acetic acid, 5-10 parts of glycerol, 5-10 parts of gelatin, 3-7 parts of nano zinc oxide, and 0.3-3 parts of silane coupling agent.

[0012] Preferably, the amino gum comprises the following raw materials in parts by weight: 3-9 parts gum polysaccharide, 3-7 parts epichlorohydrin, 3-9 parts diethylenetriamine, and 10-20 parts tetrahydrofuran.

[0013] A preferred method for preparing amino gum includes the following steps: mixing gum polysaccharide, epichlorohydrin, and diethylenetriamine, adding tetrahydrofuran, mixing again, and filtering to obtain amino gum.

[0014] Preferably, the peach gum polysaccharide is pretreated by the following steps: mixing peach gum polysaccharide and water, adjusting the pH to 3-4, filtering, and obtaining the pretreated peach gum polysaccharide.

[0015] Preferably, the mass-to-volume ratio of the peach gum polysaccharide to water is (1-2) g: 100 mL.

[0016] Preferably, the preparation method of the coating agent includes the following steps: The first part of attapulgite and γ-aminopropyltrimethoxysilane were mixed, and the first part of hydroquinone was added and mixed to obtain the first mixture; The second part of attapulgite and 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride were mixed, and the second part of hydroquinone was added and mixed to obtain the second mixture; Acrylamide and amino gum are mixed, and then the first mixture, the second mixture, and ammonium persulfate are added and mixed to obtain the third mixture; Chitosan, glacial acetic acid, and glycerin were mixed, and then gelatin and nano zinc oxide were added and mixed to obtain a fourth mixture; The third mixture, the fourth mixture, and the silane coupling agent are mixed to obtain the coating agent.

[0017] By adopting the above technical solution, this application uses polyacrylamide, peach gum polysaccharide, and attapulgite as raw materials, and mixes them with chitosan, gelatin, and nano zinc oxide through a silane coupling agent to obtain a coating agent. The added coating agent adsorbs and loads the active ingredients, which can achieve long-term sustained release of the active ingredients. At the same time, the added coating agent effectively enhances the overall performance of the bactericide through the synergistic effect with other raw materials, and further improves the safety and stability of the suspension.

[0018] This application uses polyacrylamide, peach gum polysaccharide, and attapulgite as raw materials to prepare a carrier with adsorption properties, which adsorbs active ingredients and optimizes the release behavior and safety of active ingredients. The added attapulgite adsorbs active ingredients and effectively improves the loading rate. The long-chain molecular network of the added polyacrylamide further entangles and fixes the active ingredients, reducing the loss due to rainwater erosion. The added peach gum polysaccharide slowly swells upon contact with water and gradually releases the adsorbed active ingredients through molecular diffusion, achieving long-term sustained release of active ingredients.

[0019] This application uses chitosan, gelatin, and nano-zinc oxide as raw materials. The mixture prepared has a synergistic effect with the active ingredients, which effectively enhances the overall performance of the fungicide. The added chitosan complements the fungal inhibition mechanism of prothioconazole and pyraclostrobin, thus broadening the fungicidal spectrum. The added nano-zinc oxide directly oxidizes and destroys the cell structure, and synergistically enhances the inhibition rate of fungi with prothioconazole and pyraclostrobin. At the same time, the added gelatin forms a dense film with chitosan, which can protect the active ingredients from environmental degradation.

[0020] Preferably, the emulsifier is octylphenol polyoxyethylene ether (OP-13).

[0021] Preferably, the dispersant is FTRT®DS733.

[0022] Preferably, the thickener is xanthan gum.

[0023] Preferably, the defoamer is an organosilicone defoamer (polysiloxane).

[0024] Preferably, the antifreeze is ethylene glycol.

[0025] Thirdly, this application provides a method for preparing a suspending agent, which adopts the following technical solution: A method for preparing a suspending agent includes the following steps: Weigh out each ingredient according to the formula; After mixing the coating agent, emulsifier, dispersant, thickener, defoamer, and antifreeze, the mixture is dispersed evenly by high-speed shearing. Then, prothioconazole technical, pyraclostrobin technical, and glycine technical are added and mixed. Grinding zirconium beads are added and the mixture is ultra-finely pulverized for 2-3 hours. Finally, deionized water is added and mixed to obtain a suspension containing prothioconazole, pyraclostrobin, and glycine.

[0026] In summary, this application includes at least one of the following beneficial technical effects: This application combines prothioconazole, pyraclostrobin, and glycine to obtain a compound fungicide. Prothioconazole, pyraclostrobin, and glycine have a complementary and synergistic effect, exhibiting excellent inhibition and killing effects on pathogens such as wheat scab and wheat stem rot fungus Fusarium graminearum. It has the advantages of low application concentration and broad spectrum. The suspension prepared in this application has excellent control effects on wheat stem base rot and the wheat stem base rot fungus Fusarium graminearum, preventing wheat from being attacked by pathogens during its growth, thus resulting in good growth and increased yield. Detailed Implementation

[0027] The technical solutions of this application are further illustrated by specific embodiments below. These specific embodiments do not represent a limitation on the scope of protection of this application. Any non-essential modifications and adjustments made by others based on the concept of this application still fall within the scope of protection of this application.

[0028] All raw materials involved in this application are commercially available products, among which, Peach gum polysaccharide was purchased from Taoyuan, Lijia Town, Wujin District, Changzhou City; Attapulgite clay was purchased from Jiangsu Jiuchuan Nanomaterials Technology Co., Ltd.; 1-Vinyl-3-(triethoxysilylpropyl)imidazolium chloride was prepared according to Li W, Wang F, Zhang Z, et al. Synthesis and desulfurization performance of functional silica gel modified by polymeric 1-vinyl-3-ethylimidazolium tetrafluoroborate ionic liquids[J]. Industrial & Engineering Chemistry Research, 2014, 53(43): 16664-16671; Chitosan, degree of deacetylation ≥85%, Shanghai Maclean Biochemical Technology Co., Ltd. Gelatin, Shanghai Aladdin Biochemical Technology Co., Ltd.; Nano zinc oxide, with a particle size of (50±10) nm, Shanghai Aladdin Biochemical Technology Co., Ltd. Xanthan gum, CAS No.: 11138-66-2; The present application will be further described in detail below with reference to embodiments and comparative examples.

[0029] Preparation Example 1:

[0030] The preparation method of the coating agent is as follows: (1) Preparation of amino gum Peach gum polysaccharide and deionized water were mixed at a mass-volume ratio of 1.5g:100mL for 12 hours. Concentrated hydrochloric acid was added to adjust the pH to 3. The mixture was stirred at 85℃ for 2 hours and then filtered to obtain the pretreated peach gum polysaccharide.

[0031] Dissolve 6g of pretreated peach gum polysaccharide in 60mL of N,N-dimethylacetamide, add 5g of epichlorohydrin, mix at 80℃ for 1 hour, then add 6g of diethylenetriamine and mix for 1 hour, then add 15g of tetrahydrofuran and mix for 30 minutes, filter to obtain amino peach gum.

[0032] (2) Preparation of coating agent 2g of attapulgite was dispersed in 200mL of toluene, 4g of γ-aminopropyltrimethoxysilane and 0.3g of hydroquinone were added, and the mixture was mixed at 80℃ for 10 hours under nitrogen protection. After filtration, the first mixture was obtained. 2g of attapulgite was dispersed in 200mL of toluene, 4g of 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride and 0.3g of hydroquinone were added, and the mixture was mixed at 80°C for 10 hours under nitrogen protection. The mixture was then filtered to obtain the second mixture. Mix 1g of acrylamide, 1g of amino gum, and 30mL of deionized water for 1 hour. Add the first mixture and the second mixture and mix for 30 minutes. Then add 0.03g of ammonium persulfate and mix for 30 minutes to obtain the third mixture. Mix 12g of chitosan and 12g of glacial acetic acid, add 7g of glycerol, and mix well at 50℃ to obtain a chitosan solution. Mix 8g of gelatin and 100mL of water at 50℃ to obtain a gelatin solution; After mixing the chitosan solution and gelatin solution for 25 minutes, add 5g of nano zinc oxide and mix for another 25 minutes to obtain the fourth mixture. The third mixture, the fourth mixture, 1 g of silane coupling agent KH-550, and 50 mL of deionized water were mixed for 2 hours to obtain the coating agent.

[0033] Preparation Example 2:

[0034] The preparation method of the coating agent is as follows: (1) Preparation of amino gum Peach gum polysaccharide and deionized water were mixed at a mass-volume ratio of 1.5g:100mL for 12 hours. Concentrated hydrochloric acid was added to adjust the pH to 3. The mixture was stirred at 85℃ for 2 hours and then filtered to obtain the pretreated peach gum polysaccharide.

[0035] Dissolve 3g of pretreated peach gum polysaccharide in 60mL of N,N-dimethylacetamide, add 3g of epichlorohydrin, mix at 80℃ for 1 hour, then add 3g of diethylenetriamine and mix for 1 hour, add 10g of tetrahydrofuran and mix for 30 minutes, then filter to obtain amino peach gum.

[0036] (2) Preparation of coating agent 1 g of attapulgite was dispersed in 200 mL of toluene, 2 g of γ-aminopropyltrimethoxysilane and 0.2 g of hydroquinone were added, and the mixture was mixed at 80 °C for 10 hours under nitrogen protection. The mixture was then filtered to obtain the first mixture. 1 g of attapulgite was dispersed in 200 mL of toluene, 2 g of 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride and 0.2 g of hydroquinone were added, and the mixture was mixed at 80 °C for 10 hours under nitrogen protection. The mixture was then filtered to obtain the second mixture. Mix 0.5g of acrylamide, 0.5g of amino gum, and 30mL of deionized water for 1 hour. Add the first mixture and the second mixture and mix for 30 minutes. Then add 0.01g of ammonium persulfate and mix for 30 minutes to obtain the third mixture. Mix 10g of chitosan and 10g of glacial acetic acid, add 5g of glycerol, and mix well at 50℃ to obtain a chitosan solution. Mix 5g of gelatin and 100mL of water at 50℃ to obtain a gelatin solution; After mixing the chitosan solution and gelatin solution for 25 minutes, add 3g of nano zinc oxide and mix for another 25 minutes to obtain the fourth mixture. The third mixture, the fourth mixture, 0.3 g of silane coupling agent KH-550, and 50 mL of deionized water were mixed for 2 hours to obtain the coating agent.

[0037] Preparation Example 3:

[0038] The preparation method of the coating agent is as follows: (1) Preparation of amino gum Peach gum polysaccharide and deionized water were mixed at a mass-volume ratio of 1.5g:100mL for 12 hours. Concentrated hydrochloric acid was added to adjust the pH to 3. The mixture was stirred at 85℃ for 2 hours and then filtered to obtain the pretreated peach gum polysaccharide.

[0039] Dissolve 9g of pretreated peach gum polysaccharide in 60mL of N,N-dimethylacetamide, add 7g of epichlorohydrin, mix at 80℃ for 1 hour, then add 9g of diethylenetriamine and mix for 1 hour, add 20g of tetrahydrofuran and mix for 30 minutes, then filter to obtain amino peach gum.

[0040] (2) Preparation of coating agent 3g of attapulgite was dispersed in 200mL of toluene, 6g of γ-aminopropyltrimethoxysilane and 0.4g of hydroquinone were added, and the mixture was mixed at 80℃ for 10 hours under nitrogen protection. The mixture was then filtered to obtain the first mixture. 3g of attapulgite was dispersed in 200mL of toluene, 6g of 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride and 0.4g of hydroquinone were added, and the mixture was mixed at 80°C for 10 hours under nitrogen protection. The mixture was then filtered to obtain the second mixture. Mix 1.5g of acrylamide, 1.2g of amino gum, and 30mL of deionized water for 1 hour. Add the first mixture and the second mixture and mix for 30 minutes. Then add 0.05g of ammonium persulfate and mix for 30 minutes to obtain the third mixture. Mix 15g of chitosan and 15g of glacial acetic acid, add 10g of glycerol, and mix well at 50℃ to obtain a chitosan solution. Mix 10g of gelatin and 100mL of water at 50℃ to obtain a gelatin solution; After mixing the chitosan solution and gelatin solution for 25 minutes, add 7g of nano zinc oxide and mix for another 25 minutes to obtain the fourth mixture. The third mixture, the fourth mixture, 3g of silane coupling agent KH-550, and 50mL of deionized water were mixed for 2 hours to obtain the coating agent.

[0041] Example 1:

[0042] A suspension concentrate containing prothioconazole, pyraclostrobin, and glycine, comprising the following raw materials: 150g of prothioconazole technical, 150g of pyraclostrobin technical, 150g of glycine technical, 40g of coating agent, 40g of emulsifier, 25g of dispersant, 3g of thickener, 2g of defoamer, 8g of antifreeze, and deionized water to a final volume of 1L.

[0043] The coating agent was prepared in Preparation Example 1.

[0044] The emulsifier is octylphenol polyoxyethylene ether (OP-13). The dispersant is FTRT® DS733; The thickener is xanthan gum; The defoamer is an organosilicone defoamer (polysiloxane). The antifreeze is ethylene glycol; A method for preparing a suspension containing prothioconazole, pyraclostrobin, and glycine, comprising the following steps: Weigh out each ingredient according to the formula; The coating agent, emulsifier, dispersant, thickener, defoamer, and antifreeze are mixed and dispersed evenly by high-speed shearing. Then, the technical grade of prothioconazole, pyraclostrobin, and glycine are added and mixed for 30 minutes. Grinding zirconium beads are added and the mixture is ultra-finely pulverized for 2 hours. Finally, deionized water is added and mixed for 30 minutes to obtain a suspension containing prothioconazole, pyraclostrobin, and glycine.

[0045] Example 2:

[0046] A suspension concentrate containing prothioconazole, pyraclostrobin, and glycine, comprising the following raw materials: 130g of prothioconazole technical, 130g of pyraclostrobin technical, 130g of glycine technical, 30g of coating agent, 30g of emulsifier, 20g of dispersant, 1g of thickener, 1g of defoamer, 5g of antifreeze, and deionized water to make up to 1L.

[0047] The coating agent was prepared in Preparation Example 2.

[0048] The emulsifier is octylphenol polyoxyethylene ether (OP-13). The dispersant is FTRT® DS733; The thickener is xanthan gum; The defoamer is an organosilicone defoamer (polysiloxane). The antifreeze is ethylene glycol; A method for preparing a suspension containing prothioconazole, pyraclostrobin, and glycine, comprising the following steps: Weigh out each ingredient according to the formula; The coating agent, emulsifier, dispersant, thickener, defoamer, and antifreeze are mixed and dispersed evenly by high-speed shearing. Then, the technical grade of prothioconazole, pyraclostrobin, and glycine are added and mixed for 30 minutes. Grinding zirconium beads are added and the mixture is ultra-finely pulverized for 2 hours. Finally, deionized water is added and mixed for 30 minutes to obtain a suspension containing prothioconazole, pyraclostrobin, and glycine.

[0049] Example 3:

[0050] A suspension concentrate containing prothioconazole, pyraclostrobin, and glycine, comprising the following raw materials: 170g of prothioconazole technical, 170g of pyraclostrobin technical, 170g of glycine technical, 50g of coating agent, 50g of emulsifier, 30g of dispersant, 5g of thickener, 3g of defoamer, 10g of antifreeze, and deionized water to make up to 1L.

[0051] The coating agent was prepared in Preparation Example 3.

[0052] The emulsifier is octylphenol polyoxyethylene ether (OP-13). The dispersant is FTRT® DS733; The thickener is xanthan gum; The defoamer is an organosilicone defoamer (polysiloxane). The antifreeze is ethylene glycol; A method for preparing a suspension containing prothioconazole, pyraclostrobin, and glycine, comprising the following steps: Weigh out each ingredient according to the formula; The coating agent, emulsifier, dispersant, thickener, defoamer, and antifreeze are mixed and dispersed evenly by high-speed shearing. Then, the technical grade of prothioconazole, pyraclostrobin, and glycine are added and mixed for 30 minutes. Grinding zirconium beads are added and the mixture is ultra-finely pulverized for 2 hours. Finally, deionized water is added and mixed for 30 minutes to obtain a suspension containing prothioconazole, pyraclostrobin, and glycine.

[0053] Example 4:

[0054] The difference from Example 1 is that the coating agent was prepared in Preparation Example 2.

[0055] Example 5:

[0056] The difference from Example 1 is that the coating agent was prepared in Preparation Example 3.

[0057] Example 6:

[0058] The difference from Example 1 is that the amount of coating agent added is 30g.

[0059] Example 7:

[0060] The difference from Example 1 is that the amount of coating agent added is 50g.

[0061] Comparative Example 1:

[0062] The difference from Example 1 is that no coating agent is added.

[0063] Comparative Example 2:

[0064] The difference from Example 1 is that the amount of coating agent added is 29g.

[0065] Comparative Example 3:

[0066] The difference from Example 1 is that the amount of coating agent added is 51g.

[0067] Performance testing:

[0068] 1. Stability determination Low-temperature stability test method: The test was conducted according to GB / T 19137-2003 "Determination of Low-Temperature Stability of Pesticides".

[0069] Thermal storage stability test method: The test was conducted according to GB / T 19136-2021 "Test Method for Thermal Storage Stability of Pesticides".

[0070] Table 1 Stability Testing

[0071] As shown in Table 1, the suspending agents prepared in Examples 1-7 of this application have high suspension rates and small particle size growth after heat storage, indicating that the suspending agents prepared in this application have good heat storage stability and low temperature stability.

[0072] Based on the test results of Example 1 and Comparative Example 1, it can be seen that the test results of Example 1 are better than those of Comparative Example 1, indicating that the addition of film-forming agent effectively improves the thermal storage stability and low temperature stability of the suspension.

[0073] Based on Examples 1, 6, 7, Comparative Example 2, and Comparative Example 3, it can be seen that the amount of coating agent added affects the stability test results. Furthermore, when the amount of coating agent added to each 1L of suspension is 30g-50g, the prepared suspension exhibits the best thermal storage stability and low-temperature stability.

[0074] 2. Results of antibacterial rate test 2.1 Inhibition rate against Fusarium graminearum, the causal agent of wheat blight Accurately weigh 20 mg each of the sample to be tested and prothioconazole technical, and prepare 20 mg / L solutions respectively. In a clean bench, select mycelial cakes with a diameter of 8 mm, and add the above antibacterial solution to the culture dishes containing the mycelial cakes. Repeat three times, and set up a blank control group. Place the inoculated culture dishes in a 25℃ constant temperature biochemical incubator and incubate in the dark for 5 days. After the culture is completed, measure the diameter of each colony (unit: mm) using the cross-cross method. Measure the diameter of each colony twice in the vertical direction and take the average value.

[0075] The formula for calculating the inhibition rate is: Mycelial growth inhibition rate = (Diameter of control colony - Diameter of treated colony) / Diameter of control colony × 100%; Table 2. Inhibition rate against Fusarium graminearum, the causal agent of wheat blight.

[0076] 2.2 Inhibition rate against Fusarium graminearum, the pathogen causing wheat stem rot. The in vitro toxicity of samples against *Fusarium graminearum*, the causal agent of wheat stem rot, was determined using the mycelial cake method. Weigh the test samples and prothioconazole technical grade, and dilute different samples to 6 mg / kg with sterile water. Then, use a pipette to take 2 mL of the drug solution and add it to 18 mL of PDA medium. Mix well and pour into a petri dish. Each treatment is repeated 4 times, with normal PDA medium as a control. Use a punch to make mycelial cakes with a diameter of 6 mm. Place the punched mycelial cake in the center of each cooled and solidified plate, seal with plastic wrap, and label. Incubate at 25℃ for 5 days and measure the colony diameter. After incubation, measure the diameter of each colony using the cross-sectional method. Measure the diameter of each colony twice in the vertical direction and take the average value.

[0077] The raw materials for PDA medium are as follows: 200g potato, 20g glucose, 20g agar, and water to 1000mL.

[0078] The mycelial growth inhibition rate is calculated using the following formula: Mycelial growth inhibition rate = (Diameter of control colony - Diameter of treated colony) / Diameter of control colony × 100%.

[0079] Table 3. Inhibition rate against Fusarium graminearum, the pathogen causing wheat stem rot.

[0080] Based on the results in Tables 2 and 3, it can be seen that the suspensions prepared in Examples 1-7 of this application have good antibacterial rates against Fusarium graminearum, the causal agent of wheat stem rot, and Fusarium graminearum, the causal agent of wheat stem rot.

[0081] Based on the test results of Example 1 and Comparative Example 1, it can be seen that the test results of Example 1 are better than those of Comparative Example 1, indicating that the addition of film-forming agent has a synergistic effect on the active ingredients and effectively improves the inhibition rate of suspension against Fusarium graminearum and Fusarium graminearum, the causal agent of wheat stem rot.

[0082] Based on Examples 1, 6, 7, Comparative Example 2, and Comparative Example 3, it can be seen that the amount of coating agent added affects the stability test results, and the antibacterial rate of the prepared suspension is optimal when the amount of coating agent added in each 1L of suspension is 30g-50g.

[0083] Production increase rate test results Wheat seeds were coated with the suspension concentrate at a dosage of 10g of the concentrate per 10kg of seeds. Seeds coated with water served as a blank control. After coating, the seeds were dried and stored for later use. Fields with a history of wheat stem rot and wheat scab, consistent previous crop, soil pH of 6.0-7.0, uniform fertility, and level terrain were selected as test sites. A randomized block design was used: three replicates (three blocks), each block containing five treatments (randomized), with a plot area of ​​30m². 2 (5m×6m, to reduce marginal effects), row spacing 25cm, seeding rate 150kg / hm² (following local field standards). Wheat field cultivation management includes fertilization, irrigation, and pest and disease control, except for the target diseases, consistent with local field practices. The focus is on recording the control efficacy and yield indicators of each treatment against stem base rot and Fusarium head blight.

[0084] Yield measurement was conducted at harvest time: 5 points were sampled along the diagonal of each plot, and 1 m³ of actual yield was measured at each point. 2 All plants within the area were threshed and weighed to calculate the yield per hectare. The yield increase rate (%) was calculated using the formula: [(yield of treatment area - yield of control area) / yield of control area] × 100%.

[0085] Table 4 Results of Yield Increase Detection

[0086] As can be seen from Tables 2, 3 and 4, the suspensions prepared in Examples 1-7 of this application have good antibacterial effects against Fusarium graminearum and wheat stem rot fungus, and have excellent control effects against Fusarium graminearum and wheat stem rot fungus, so that wheat will not be attacked by pathogens during the growth process, thus showing good growth and increasing yield.

[0087] Based on the test results of Example 1 and Comparative Example 1, it can be seen that the test results of Example 1 are better than those of Comparative Example 1, indicating that the addition of the film-forming agent has excellent control effect on wheat scab and wheat stem rot fungus, and will not affect wheat yield.

[0088] Based on Examples 1, 6, 7, Comparative Example 2, and Comparative Example 3, it can be seen that the amount of coating agent added affects the stability test results, and the growth rate of wheat is best when the amount of coating agent added in each 1L of suspension is 30g-50g.

Claims

1. A pesticide composition, characterized in that: It includes active ingredient A, active ingredient B, and active ingredient C, wherein active ingredient A is prothioconazole; active ingredient B is pyraclostrobin; and active ingredient C is glycine.

2. The pesticide composition according to claim 1, characterized in that: The mass concentration of the prothioconazole is 130-170 g / L; the mass concentration of the pyraclostrobin is 130-170 g / L; and the mass concentration of the glycine is 130-170 g / L.

3. A suspending agent, characterized in that: Including the following raw materials: The pesticide composition according to claim 1 or 2; Coating agent 30-50 g / L; Emulsifier 30-50g / L; Dispersant 20-30 g / L; Thickener 1-5g / L; Defoamer 1-3g / L; Antifreeze 5-10g / L.

4. A suspending agent according to claim 3, characterized in that: The coating agent comprises the following raw materials in parts by weight: 0.5-1.2 parts of amino gum, 1-3 parts of attapulgite, 2-6 parts of γ-aminopropyltrimethoxysilane, 0.2-0.4 parts of hydroquinone, 1-3 parts of attapulgite, 2-6 parts of 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride, 0.2-0.4 parts of hydroquinone, 0.5-1.5 parts of acrylamide, 0.01-0.05 parts of ammonium persulfate, 10-15 parts of chitosan, 10-15 parts of glacial acetic acid, 5-10 parts of glycerol, 5-10 parts of gelatin, 3-7 parts of nano zinc oxide, and 0.3-3 parts of silane coupling agent.

5. A suspending agent according to claim 4, characterized in that: The amino gum comprises the following raw materials in parts by weight: 3-9 parts gum polysaccharide, 3-7 parts epichlorohydrin, 3-9 parts diethylenetriamine, and 10-20 parts tetrahydrofuran.

6. A suspending agent according to claim 5, characterized in that: The preparation method of amino gum is as follows: mix gum polysaccharide, epichlorohydrin, and diethylenetriamine, add tetrahydrofuran and mix, filter to obtain amino gum.

7. A suspending agent according to claim 5 or 6, characterized in that: The pretreatment of peach gum polysaccharide is carried out by the following steps: mix peach gum polysaccharide and water, adjust the pH to 3-4, filter, and obtain pretreated peach gum polysaccharide.

8. A suspending agent according to claim 7, characterized in that: The mass-to-volume ratio of the peach gum polysaccharide to water is (1-2) g: 100 mL.

9. A suspending agent according to claim 3, characterized in that: The preparation method of the coating agent includes the following steps: The first part of attapulgite and γ-aminopropyltrimethoxysilane were mixed, and the first part of hydroquinone was added and mixed to obtain the first mixture; The second part of attapulgite and 1-vinyl-3-(triethoxysilylpropyl)imidazolium chloride were mixed, and the second part of hydroquinone was added and mixed to obtain the second mixture; Acrylamide and amino gum are mixed, and then the first mixture, the second mixture, and ammonium persulfate are added and mixed to obtain the third mixture; Chitosan, glacial acetic acid, and glycerin were mixed, and then gelatin and nano zinc oxide were added and mixed to obtain a fourth mixture; The third mixture, the fourth mixture, and the silane coupling agent are mixed to obtain the coating agent.

10. A method for preparing a suspending agent, characterized in that: Includes the following steps: Weigh out each ingredient according to the formula; The coating agent, emulsifier, dispersant, thickener, defoamer, and antifreeze are mixed and sheared to disperse. Then, prothioconazole, pyraclostrobin, and glycine are added and mixed. Grinding is carried out by adding zirconium beads and then adding deionized water to obtain a suspension containing prothioconazole, pyraclostrobin, and glycine.

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