Retinol microcapsule bead anti-wrinkle tightening triple collagen essence cream and preparation method thereof

Abstract

The application relates to the cosmetic technical field, in particular to a retinol microcapsule condensation pearl anti-wrinkle firming triple collagen essence cream and a preparation method thereof. The essence cream provided in the application realizes the balance of retinol slow release stability, mild low stimulation, whole-layer collagen activation and barrier repair performance, and avoids the problems of easy oxidation failure of existing retinol skin care products, relatively high irritability of raw materials, weak transdermal absorption of ordinary collagen products, single anti-aging dimension of single active ingredient, low purification efficiency and insufficient activity of conventional plant extracts. The essence cream provided in the application can be used for facial skin anti-wrinkle firming and daily barrier repair and maintenance, and is suitable for the use requirements of long-acting anti-aging, mild stability maintenance and multi-dimensional skin repair of mature skin and sensitive skin.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and in particular to a retinol microcapsule anti-wrinkle and firming triple collagen essence cream and its preparation method. Background Technology

[0002] Anti-aging and repair, as a core development track in the cosmetics industry, has become a key direction for upgrading and innovation in the beauty and skincare field due to its advantages of a wide audience, inelastic demand, and rapid technological iteration. The global market for functional skincare continues its rapid growth, with consumers increasingly demanding anti-wrinkle, firming, and repairing effects. Anti-aging products that combine high performance, high temperature resistance, and high stability have become the mainstream choice in the market, leading to a continuous expansion of the high-end functional skincare market and a steady acceleration in product innovation and technological upgrades. Among these, retinol, recognized as the gold standard ingredient for anti-aging, combined with recombinant collagen to synergistically revitalize the skin's collagen structure, has become a mainstream research and development direction for anti-wrinkle and firming skincare products. The active ingredient compounding system and gentle delivery technology determine the efficacy release, skin feel, and long-term safety of skincare products, representing the core technologies of high-end anti-aging skincare products.

[0003] As consumers' skincare needs become increasingly refined and functional, anti-aging skincare products are gradually upgrading towards high activity, low irritation, strong penetration, and long-lasting effects. The increase in retinol content, the diversification of collagen types, and the combination of plant active ingredients have become mainstream trends, placing more stringent requirements on the stability of skincare product ingredients, skin compatibility, and synergistic effects. While existing retinol-based anti-aging products offer some anti-wrinkle effects, retinol itself is prone to oxidation and deactivation, has strong irritant properties, and is susceptible to incompatibility when combined with collagen ingredients, making stable coexistence difficult. Conventional collagen skincare products suffer from poor transdermal absorption, easy degradation of active ingredients, and the inability of single collagen to cover the entire anti-aging layer, making it difficult to simultaneously address the multiple needs of epidermal repair, basement membrane stabilization, and dermal collagen regeneration. Furthermore, conventional formulas generally suffer from uncontrollable release of active ingredients, insufficient soothing and repair capabilities, and a sticky texture, failing to meet the multiple compatibility requirements of high-end anti-aging skincare products, which include gentleness and non-irritation, high-efficiency collagen promotion, stable and easy absorption, and a refreshing feel. This severely restricts the efficacy upgrade and market penetration of high-end anti-wrinkle and firming skincare products.

[0004] To address the aforementioned technical shortcomings, there is an urgent need to improve existing technologies. Summary of the Invention

[0005] In view of this, this application provides a retinol microcapsule anti-wrinkle and firming triple collagen essence cream and its preparation method. This essence cream achieves a balance of gentleness and low irritation, activity stability, full-layer collagen production promotion, and anti-wrinkle and firming skin feel, while avoiding the problems of easy oxidation and irritation, poor transdermal absorption of reconstituted collagen, and difficulty in synergistic effects of multiple active ingredients in existing retinol skincare products. The essence cream provided by this application can be used for facial anti-wrinkle and firming skincare and barrier repair, meeting various skincare needs.

[0006] In the first aspect, this application discloses a retinol microcapsule anti-wrinkle and firming triple collagen essence cream, the technical solution of which is as follows:

[0007] A retinol microcapsule anti-wrinkle and firming triple collagen essence cream, by weight, is composed of the following ingredients: 0.15-0.20 parts of microcapsule-encapsulated retinol, 0.20-0.30 parts of Centella asiatica extract, 0.20-0.25 parts of sophorolipid, 0.10-0.15 parts of Aspergillus fermentation product, 0.10-0.15 parts of type I collagen, 0.10-0.15 parts of recombinant type III humanized collagen, 0.0001-0.0005 parts of recombinant type XVII collagen, 12-12.5 parts of moisturizer, 0.35-0.36 parts of thickening and stabilizing agent, 1.0-1.2 parts of preservative, 19-20 parts of oil phase, and 65-75 parts of deionized water.

[0008] Optionally, the preparation method of the Centella asiatica extract includes the following steps:

[0009] Centella asiatica whole herb powder and ethanol aqueous solution were mixed and subjected to ultrasonic extraction to obtain Centella asiatica extract.

[0010] The Centella asiatica extract, citric acid, and malic acid were degraded to obtain a degradation solution;

[0011] The degradation solution was subjected to centrifugation, adsorption, purification, concentration and freeze-drying in sequence to obtain the Centella asiatica extract.

[0012] Optionally, the mass-to-volume ratio of the whole Centella asiatica herb powder to the ethanol aqueous solution is 1g:(18~22)mL, and the mass fraction of the ethanol aqueous solution is 45%~55%.

[0013] Optionally, the ultrasonic extraction process is performed at a frequency of 20kHz to 30kHz, a power of 250W to 350W, an extraction temperature of 35℃ to 40℃, and an extraction time of 80min to 100min.

[0014] Optionally, the mass ratio of citric acid to malic acid is 2-3:1, and the total amount of citric acid and malic acid added is 0.5%-0.8% of the mass of Centella asiatica extract.

[0015] Optionally, the degradation treatment is carried out at a temperature of 45℃~50℃, a pH value of 3.0~3.5, and a degradation time of 80min~90min.

[0016] Optionally, the centrifugation speed is 7000 r / min to 9000 r / min, and the centrifugation time is 10 min to 20 min.

[0017] Optionally, the adsorption treatment temperature is 35℃~45℃, the adsorption time is 50min~90min, and the amount of adsorbent added is 1.5%~3.5% of the mass of the degradation liquid.

[0018] Optionally, the purification process uses D101 type macroporous resin and elutes with a gradient of ethanol at volume fractions of 20%~30%, 50%~60%, and 75%~85%.

[0019] Optionally, the concentration process is low-temperature vacuum concentration, with a concentration temperature of 45℃~55℃ and a vacuum degree of -0.08MPa~-0.1MPa.

[0020] Optionally, the freeze-drying temperature is -55℃ to -45℃, and the drying time is 20h to 28h.

[0021] Optionally, the method for preparing the adsorbent used in the adsorption treatment includes the following steps:

[0022] After mixing attapulgite clay, chitosan and food-grade activated carbon, the mixture is stirred to obtain a composite powder.

[0023] The composite powder is mixed with the activation liquid and then subjected to an immersion activation treatment to obtain an activated composite material.

[0024] The activated composite material was washed, dried, and pulverized in sequence to obtain the self-made composite adsorbent.

[0025] Optionally, the mass ratio of attapulgite, chitosan, and food-grade activated carbon is 10:5~7:3~5.

[0026] Optionally, the mass-to-volume ratio of the composite powder to the activation solution is 1 g: (25~35) mL, and the activation solution is a sodium bicarbonate aqueous solution with a mass fraction of 0.8%~1.2%.

[0027] Optionally, the temperature of the immersion activation treatment is 20℃~30℃, and the activation time is 25min~35min.

[0028] Optionally, the drying temperature is 100℃~110℃ and the time is 1.5h~2.5h.

[0029] Optionally, the mesh size of the pulverized powder is 80 to 120 mesh.

[0030] Optionally, the method for preparing the microencapsulated retinol includes the following steps:

[0031] Chitosan, gum arabic, and deionized water are mixed and stirred to dissolve, thus obtaining a composite wall material liquid.

[0032] Retinol was added to the composite wall material liquid, and shearing and homogenization were performed sequentially to obtain a microcapsule emulsion.

[0033] The microcapsule emulsion was spray-dried to obtain the microcapsule-encapsulated retinol.

[0034] Optionally, the mass ratio of chitosan to gum arabic is (0.8~1.2):(0.6~1.0).

[0035] Optionally, the mass-volume ratio of chitosan, gum arabic, and deionized water is (0.8~1.2) g : (0.6~1.0) g : (80~120) mL.

[0036] Optionally, the stirring and dissolving temperature is 35℃~45℃, the stirring speed is 200r / min~300r / min, and the time is 20min~40min.

[0037] Optionally, the mass-to-volume ratio of retinol to composite wall material liquid is 1g:(80~120)mL.

[0038] Optionally, the rotational speed of the shearing process is 2500 r / min to 3500 r / min, and the shearing time is 8 min to 12 min.

[0039] Optionally, the homogenization process is carried out at a pressure of 50 MPa to 70 MPa, and the homogenization is performed 2 to 4 times.

[0040] Optionally, the inlet air temperature of the spray drying process is 110℃~130℃, and the outlet air temperature is 55℃~65℃.

[0041] Optionally, the sophorolipid is selected from one or both of lactone-type sophorolipids and acid-type sophorolipids.

[0042] Optionally, the moisturizer is selected from one or more of glycerin, propylene glycol, sodium hyaluronate, panthenol, allantoin, or erythritol.

[0043] Optionally, the thickening stabilizer is selected from one or both of carbomer and triethanolamine.

[0044] Optionally, the preservative is selected from one or both of p-hydroxyacetophenone or 1,2-hexanediol.

[0045] Optionally, the oil phase component is selected from one or more of ethylhexyl stearate, ethylhexyl palmitate, behenol, arachidonic acid, ethylhexyl hydroxystearate, or stearic acid.

[0046] Secondly, this application discloses a method for preparing the retinol microcapsule anti-wrinkle and firming triple collagen essence cream described in the aforementioned scheme, including the following steps:

[0047] The humectant, thickener, stabilizer, preservative and deionized water are mixed, heated and stirred to obtain an aqueous phase material.

[0048] The oil phase components are heated and melted to obtain the oil phase material;

[0049] The oil phase material is added to the aqueous phase material, homogenized and emulsified, and then the pH value is adjusted to 6.0~6.5 to obtain an emulsified base.

[0050] The retinol microcapsule-encapsulated retinol, centella asiatica extract, sophorolipid, Aspergillus fermentation product, type I collagen, recombinant type III humanized collagen and recombinant type XVII collagen were added to the emulsion base and stirred to disperse, thus obtaining the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

[0051] Optionally, the heating and stirring temperature is 80~85℃, the rotation speed is 200r / min~300r / min, and the time is 20min~30min.

[0052] Optionally, the heating and melting temperature is 80~85℃, the rotation speed is 150r / min~200r / min, and the time is 15min~25min.

[0053] Optionally, the homogenization emulsification rotation speed is 3000 r / min to 3500 r / min, and the time is 5 min to 10 min.

[0054] Optionally, the stirring and dispersing speed is 200 r / min to 250 r / min, and the time is 25 min to 35 min.

[0055] The retinol microcapsule anti-wrinkle and firming triple collagen essence cream provided in this application is suitable for skincare needs that combine anti-wrinkle firming and barrier repair. It solves the problems of existing retinol skincare products, such as easy oxidation and inactivation, strong irritation, and difficulty in transdermal absorption of reconstituted collagen. It also avoids problems caused by formula and process defects, such as unstable activity, single efficacy, skin irritation, insufficient collagen regeneration, and weak barrier repair. Therefore, compared with existing technologies, the retinol microcapsule anti-wrinkle and firming triple collagen essence cream provided in this application has the advantages of stable and controllable activity, gentle and low irritation, full-layer collagen activation, highly effective anti-wrinkle and firming, strong barrier repair, and a refreshing and comfortable feel, meeting consumers' needs for highly effective anti-aging, gentle repair, long-lasting stabilization, and cost-effective skincare. Detailed Implementation

[0056] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. In addition, it should be understood that the specific implementation methods described herein are only for illustration and explanation of this application and are not intended to limit this application.

[0057] In this application, unless otherwise stated, the term "comprising" means "including but not limited to". The terms first, second, third, etc., are used merely as illustrative purposes and do not impose numerical requirements or establish an order.

[0058] In this application, "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural.

[0059] In this application, "at least one" means one or more, and "more than one" means two or more. "One or more", "at least one of the following", or similar expressions refer to any combination of these items, including any combination of single or multiple items. For example, "at least one of a, b, or c", or "at least one of a, b, and c" can both mean: a, b, c, a~b (i.e., a and b), a~c, b~c, or a~b~c, where a, b, and c can be single or multiple.

[0060] Various embodiments of this application may exist in the form of a range; it should be understood that the description in the form of a range is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of this application; therefore, it should be considered that the scope description of this application has specifically disclosed all possible sub-ranges and single numerical values ​​within that range. For example, it should be considered that the scope description from 1 to 6 has specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Furthermore, whenever a numerical range is referred to herein, it means including any referenced number (fraction or integer) within the referred range.

[0061] The present application will be specifically described below through specific embodiments. The following embodiments are only some embodiments of the present application and are not intended to limit the present application.

[0062] In the first aspect, this application discloses a retinol microcapsule anti-wrinkle and firming triple collagen essence cream, the technical solution of which is as follows:

[0063] A retinol microcapsule anti-wrinkle and firming triple collagen essence cream, by weight, is composed of the following ingredients: 0.15-0.20 parts of microcapsule-encapsulated retinol, 0.20-0.30 parts of Centella asiatica extract, 0.20-0.25 parts of sophorolipid, 0.10-0.15 parts of Aspergillus fermentation product, 0.10-0.15 parts of type I collagen, 0.10-0.15 parts of recombinant type III humanized collagen, 0.0001-0.0005 parts of recombinant type XVII collagen, 12-12.5 parts of moisturizer, 0.35-0.36 parts of thickening and stabilizing agent, 1.0-1.2 parts of preservative, 19-20 parts of oil phase, and 65-75 parts of deionized water.

[0064] The retinol microcapsule anti-wrinkle and firming triple collagen essence cream provided in this application uses retinol encapsulated in microcapsules as the core anti-aging component. Type I / recombinant type III / recombinant type XVII collagen constructs a full-layer collagen support network. Centella asiatica extract, sophorolipid, and Aspergillus fermentation products work together to soothe inflammation and provide endogenous antioxidant effects. Moisturizers, oil phase components, thickeners, stabilizers, and preservatives respectively ensure skin feel, stability, and gentleness. Through the combination and synergistic effect of each component, a balanced improvement in performance is achieved.

[0065] In this application, type I collagen, as structural collagen, provides skin support and firmness; recombinant type III humanized collagen can improve skin elasticity, repair damage, and improve roughness; recombinant type XVII collagen can stabilize the epidermal-dermal connection and delay skin sagging; the combination of the three can achieve full-layer anti-aging by repairing the epidermis, strengthening the basement membrane, and plumping the dermis, making up for the shortcomings of single collagen that only works on a single layer.

[0066] In this application, the Centella Asiatica extract quickly relieves skin irritation caused by retinol, reducing redness and stinging, while also promoting skin damage repair, assisting in enhancing collagen synthesis, and ensuring the gentleness of the formula.

[0067] In this application, the sophorolipid is selected from one or two of lactone-type sophorolipids and acid-type sophorolipids, and can exert natural anti-inflammatory, antibacterial, soothing, antipruritic, and emulsifying effects.

[0068] In this application, the Aspergillus fermentation product is an endogenous antioxidant active component that can activate the skin's antioxidant pathway, scavenge free radicals, inhibit oxidative stress, reduce collagen degradation, dull skin tone, and fine line formation caused by photoaging and free radicals, and work synergistically with retinol to combat photoaging, while improving skin yellowing and dullness, and enhancing brightening and anti-aging effects.

[0069] In this application, the moisturizer can achieve deep hydration, long-lasting moisture retention, and repair of the skin barrier.

[0070] In this application, the oil phase component can optimize the spreadability, moisturizing effect and skin feel of the cream, form a thin protective film on the epidermis, reduce moisture loss, and work synergistically with the moisturizer to build an internal and external water-locking system.

[0071] In this application, the thickening stabilizer can adjust the consistency, stability and skin feel of the paste, making the paste delicate and uniform, without layering or water separation, while ensuring that the active ingredients are stably dispersed in the system and avoiding aggregation and failure.

[0072] In this application, the preservative can achieve mild preservation and extend the product shelf life.

[0073] In this application, the deionized water serves as a dispersion medium, ensuring that all active ingredients, moisturizing components, oil phase components, etc., are fully mixed and dissolved to form a stable and uniform cream system. At the same time, it regulates the refreshingness of the cream, avoiding it from being too thick or too thin, making it suitable for daily use on facial skin.

[0074] In some embodiments, the method for preparing the Centella asiatica extract includes the following steps:

[0075] Centella asiatica whole herb powder and ethanol aqueous solution were mixed and subjected to ultrasonic extraction to obtain Centella asiatica extract.

[0076] The Centella asiatica extract, citric acid, and malic acid were degraded to obtain a degradation solution;

[0077] The degradation solution was subjected to centrifugation, adsorption, purification, concentration and freeze-drying in sequence to obtain the Centella asiatica extract.

[0078] In some embodiments, the mass-to-volume ratio of the whole Centella asiatica herb powder to the ethanol aqueous solution is 1 g: (18~22) mL, and the mass fraction of the ethanol aqueous solution is 45%~55%.

[0079] In some embodiments, the ultrasonic extraction process is performed at a frequency of 20kHz to 30kHz, a power of 250W to 350W, an extraction temperature of 35℃ to 40℃, and an extraction time of 80min to 100min.

[0080] In some embodiments, the mass ratio of citric acid to malic acid is 2-3:1, and the total amount of citric acid and malic acid added is 0.5%-0.8% of the mass of Centella asiatica extract.

[0081] In some embodiments, the degradation treatment temperature is 45℃~50℃, the system pH value is 3.0~3.5, and the degradation time is 80min~90min.

[0082] In some embodiments, the centrifugation speed is 7000 r / min to 9000 r / min, and the centrifugation time is 10 min to 20 min.

[0083] In some embodiments, the adsorption treatment temperature is 35℃~45℃, the adsorption time is 50min~90min, and the amount of adsorbent added is 1.5%~3.5% of the mass of the degradation liquid.

[0084] In some embodiments, the purification process uses D101 type macroporous resin and elutes with a gradient of ethanol at volume fractions of 20%~30%, 50%~60%, and 75%~85%.

[0085] In some embodiments, the concentration process is low-temperature vacuum concentration, with a concentration temperature of 45°C to 55°C and a vacuum degree of -0.08MPa to -0.1MPa.

[0086] In some embodiments, the freeze-drying temperature is -55℃ to -45℃, and the drying time is 20h to 28h.

[0087] In some embodiments, the preparation method of the self-made composite adsorbent used in the adsorption treatment includes the following steps:

[0088] After mixing attapulgite clay, chitosan and food-grade activated carbon, the mixture is stirred to obtain a composite powder.

[0089] The composite powder is mixed with the activation liquid and then subjected to an immersion activation treatment to obtain an activated composite material.

[0090] The activated composite material was washed, dried, and pulverized in sequence to obtain the self-made composite adsorbent.

[0091] In some embodiments, the mass ratio of attapulgite, chitosan, and food-grade activated carbon is 10:5~7:3~5.

[0092] In some embodiments, the mass-to-volume ratio of the composite powder to the activation solution is 1 g: (25~35) mL, and the activation solution is a sodium bicarbonate aqueous solution with a mass fraction of 0.8%~1.2%.

[0093] In some embodiments, the temperature of the immersion activation treatment is 20°C to 30°C, and the activation time is 25 min to 35 min.

[0094] In some embodiments, the drying temperature is 100℃~110℃ and the time is 1.5h~2.5h.

[0095] In some embodiments, the mesh size of the pulverized powder is 80 to 120 mesh.

[0096] In some embodiments, the method for preparing the microencapsulated retinol includes the following steps:

[0097] Chitosan, gum arabic, and deionized water are mixed and stirred to dissolve, thus obtaining a composite wall material liquid.

[0098] Retinol was added to the composite wall material liquid, and shearing and homogenization were performed sequentially to obtain a microcapsule emulsion.

[0099] The microcapsule emulsion was spray-dried to obtain the microcapsule-encapsulated retinol.

[0100] In some embodiments, the mass ratio of chitosan to gum arabic is (0.8~1.2):(0.6~1.0).

[0101] In some embodiments, the mass-to-volume ratio of chitosan, gum arabic, and deionized water is (0.8~1.2) g : (0.6~1.0) g : (80~120) mL.

[0102] In some embodiments, the stirring and dissolving temperature is 35℃~45℃, the stirring speed is 200r / min~300r / min, and the time is 20min~40min.

[0103] In some embodiments, the mass-to-volume ratio of retinol to composite wall material liquid is 1 g: (80~120) mL.

[0104] In some embodiments, the rotational speed of the shearing process is 2500 r / min to 3500 r / min, and the shearing time is 8 min to 12 min.

[0105] In some embodiments, the homogenization process is carried out at a pressure of 50 MPa to 70 MPa, and the number of homogenization cycles is 2 to 4.

[0106] In some embodiments, the inlet air temperature of the spray drying process is 110°C to 130°C, and the outlet air temperature is 55°C to 65°C.

[0107] In some embodiments, the sophorolipid is selected from one or both of lactone-type sophorolipids and acid-type sophorolipids.

[0108] In some embodiments, the moisturizer is selected from one or more of glycerin, propylene glycol, sodium hyaluronate, panthenol, allantoin, or erythritol.

[0109] In some embodiments, the thickening stabilizer is selected from one or both of carbomer and triethanolamine.

[0110] In some embodiments, the preservative is selected from one or both of p-hydroxyacetophenone or 1,2-hexanediol.

[0111] In some embodiments, the oil phase component is selected from one or more of ethylhexyl stearate, ethylhexyl palmitate, behenol, arachidonic acid, ethylhexyl hydroxystearate, or stearic acid.

[0112] Secondly, this application discloses a method for preparing the above-mentioned retinol microcapsule anti-wrinkle and firming triple collagen essence cream, including the following steps:

[0113] The humectant, thickener, stabilizer, preservative and deionized water are mixed, heated and stirred to obtain an aqueous phase material;

[0114] The oil phase components are heated and melted to obtain the oil phase material;

[0115] The oil phase material is added to the aqueous phase material, homogenized and emulsified, and then the pH value is adjusted to 6.0~6.5 to obtain an emulsified base.

[0116] Retinol, Centella asiatica extract, sophorolipid, Aspergillus fermentation product, type I collagen, recombinant type III humanized collagen and recombinant type XVII collagen were added to the emulsion base and stirred and dispersed to prepare the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

[0117] It should be noted that the preparation method of this application adopts a process of first preparing the aqueous phase and oil phase separately, then emulsifying to form a base, and finally adding the active ingredients, which can effectively avoid problems such as deactivation of active ingredients, layering of the paste, and uneven dispersion.

[0118] In some embodiments, the heating and stirring temperature is 80~85℃, the rotation speed is 200r / min~300r / min, and the time is 20min~30min.

[0119] In some embodiments, the heating and melting temperature is 80~85℃, the rotation speed is 150r / min~200r / min, and the time is 15min~25min.

[0120] In some embodiments, the homogenization emulsification rotation speed is 3000 r / min to 3500 r / min, and the time is 5 min to 10 min.

[0121] In some embodiments, the stirring and dispersion speed is 200 r / min to 250 r / min, and the time is 25 min to 35 min.

[0122] The present application will be specifically described below through specific embodiments. The following embodiments are only some embodiments of the present application and are not intended to limit the present application.

[0123] Example 1

[0124] This embodiment provides a method for preparing a retinol microcapsule anti-wrinkle and firming triple collagen essence cream, including the following steps:

[0125] Mix 12.2g of humectant, 0.355g of thickener and stabilizer, 1.1g of preservative with 70g of deionized water, and heat and stir at 82℃ and 250r / min for 25min to obtain an aqueous phase material.

[0126] 19.55g of ethylhexyl stearate was heated to 82℃ and 180r / min for 20min to obtain the oil phase material;

[0127] The oil phase material was added to the aqueous phase material and homogenized and emulsified at 3200 r / min for 8 min. After cooling, the pH value was adjusted to 6.0~6.5 to obtain the emulsified base.

[0128] 0.175g of microencapsulated retinol, 0.25g of Centella asiatica extract, 0.225g of sophorolipid, 0.125g of Aspergillus fermentation product, 0.125g of type I collagen, 0.125g of recombinant type III humanized collagen, and 0.0003g of recombinant type XVII collagen were added to an emulsion base and dispersed by stirring at 220r / min for 30min to obtain the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

[0129] The preparation method of the Centella asiatica extract includes the following steps:

[0130] Mix 10g of Centella asiatica whole herb powder with 200mL of 50% ethanol aqueous solution, and extract at 38℃ for 90min using low-temperature ultrasound at 25kHz and 300W to obtain Centella asiatica extract.

[0131] Citric acid and malic acid were added to the Centella asiatica extract at a mass ratio of 2.5:1, with a total addition of 0.65% of the extract mass. The extract was then degraded at 48℃ and pH 3.2 for 85 minutes to obtain the degradation solution.

[0132] The degradation solution was centrifuged at 8000 r / min for 15 min, and the supernatant was collected. 2.5% of the adsorbent by mass of the degradation solution was added, and adsorption was carried out at 40℃ for 70 min. After filtration, the solution was eluted with a gradient of 25%, 55%, and 80% ethanol by volume fraction using D101 macroporous resin. The eluent was concentrated under low temperature reduced pressure at 50℃ and -0.09 MPa, and then freeze-dried at -50℃ for 24 h to obtain Centella asiatica extract.

[0133] The method for preparing the adsorbent includes the following steps:

[0134] 10g of attapulgite, 6g of chitosan and 4g of food-grade activated carbon were mixed and stirred to obtain composite powder.

[0135] 10g of composite powder was mixed with 300mL of sodium bicarbonate activation solution with a mass fraction of 1.0%, and the mixture was soaked and activated at 25℃ for 30min to obtain activated composite material.

[0136] After washing the activated composite material to neutrality, it was dried at 105℃ for 2 hours, pulverized and passed through a 100-mesh sieve to obtain the self-made composite adsorbent.

[0137] The method for preparing the microencapsulated retinol includes the following steps:

[0138] Mix 1g chitosan, 0.8g gum arabic with 100mL deionized water, and stir at 40℃ and 250r / min for 30min to dissolve, thus obtaining the composite wall material liquid;

[0139] Add 1g of retinol to 100mL of composite wall material solution, shear at 3000r / min for 10min, and then homogenize at 60MPa for 3 times to obtain microcapsule emulsion.

[0140] Microencapsulated retinol was prepared by spray drying the microencapsulated emulsion at an inlet air temperature of 120°C and an outlet air temperature of 60°C.

[0141] Example 2

[0142] This embodiment provides a method for preparing a retinol microcapsule anti-wrinkle and firming triple collagen essence cream, including the following steps:

[0143] Mix 12.3g of humectant, 0.36g of thickener and stabilizer, 1.2g of preservative with 75g of deionized water, and heat and stir at 85℃ and 300r / min for 20min to obtain an aqueous phase material.

[0144] 20g of ethylhexyl palmitate was heated to 85℃ and 200r / min for 15min to obtain the oil phase material;

[0145] The oil phase material was added to the aqueous phase material and homogenized and emulsified at 3500 r / min for 5 min. After cooling, the pH value was adjusted to 6.0~6.5 to obtain the emulsion base.

[0146] 0.20g of microencapsulated retinol, 0.30g of Centella asiatica extract, 0.25g of sophorolipid, 0.15g of Aspergillus fermentation product, 0.15g of type I collagen, 0.15g of recombinant type III humanized collagen, and 0.0005g of recombinant type XVII collagen were added to an emulsion base and stirred and dispersed at 250r / min for 25min to obtain the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

[0147] The preparation method of the Centella asiatica extract includes the following steps:

[0148] Mix 10g of Centella asiatica whole herb powder with 220mL of 55% ethanol aqueous solution, and extract with low temperature ultrasound at 30kHz and 350W at 40℃ for 100min to obtain Centella asiatica extract.

[0149] Citric acid and malic acid were added to the Centella asiatica extract at a mass ratio of 3:1, with a total addition of 0.8% of the extract mass. The extract was then degraded at 50°C and pH 3.5 for 90 min to obtain the degradation solution.

[0150] The degradation solution was centrifuged at 9000 r / min for 10 min, and the supernatant was collected. 3.5% of the adsorbent by mass of the degradation solution was added, and adsorption was carried out at 45℃ for 50 min. After filtration, the solution was eluted with a gradient of 30%, 60%, and 85% ethanol by volume fraction using D101 macroporous resin. The eluent was concentrated under low temperature reduced pressure at 55℃ and -0.1 MPa, and then freeze-dried at -45℃ for 20 h to obtain Centella asiatica extract.

[0151] The method for preparing the adsorbent includes the following steps:

[0152] 10g of attapulgite, 7g of chitosan and 5g of food-grade activated carbon were mixed and stirred to obtain composite powder.

[0153] 10g of composite powder was mixed with 350mL of sodium bicarbonate activation solution with a mass fraction of 1.2%, and the mixture was soaked and activated at 30℃ for 25min to obtain activated composite material.

[0154] After washing the activated composite material to neutrality, it was dried at 110℃ for 1.5 hours and then pulverized through a 120-mesh sieve to obtain the self-made composite adsorbent.

[0155] The method for preparing the microencapsulated retinol includes the following steps:

[0156] Mix 1.2g chitosan, 1.0g gum arabic with 120mL deionized water, and stir at 45℃ and 300r / min for 20min to dissolve, thus obtaining the composite wall material liquid;

[0157] Add 1g of retinol to 120mL of composite wall material liquid, shear at 3500r / min for 8min, and then homogenize twice under high pressure of 70MPa to obtain microcapsule emulsion.

[0158] Microencapsulated retinol was prepared by spray drying the microencapsulated emulsion at an inlet air temperature of 130°C and an outlet air temperature of 65°C.

[0159] Example 3

[0160] This embodiment provides a method for preparing a retinol microcapsule anti-wrinkle and firming triple collagen essence cream, including the following steps:

[0161] Mix 12.1g of humectant, 0.35g of thickener and stabilizer, 1.0g of preservative with 65g of deionized water, and heat and stir at 80℃ and 200r / min for 30min to obtain an aqueous phase material.

[0162] 19.05 g of behenol was heated at 80 °C and 150 r / min for 25 min to melt, yielding an oil phase material;

[0163] The oil phase material was added to the aqueous phase material and homogenized and emulsified at 3000 r / min for 10 min. After cooling, the pH value was adjusted to 6.0~6.5 to obtain the emulsified base.

[0164] 0.15g of microencapsulated retinol, 0.20g of Centella asiatica extract, 0.20g of sophorolipid, 0.10g of Aspergillus fermentation product, 0.10g of type I collagen, 0.10g of recombinant type III humanized collagen, and 0.0001g of recombinant type XVII collagen were added to an emulsion base and stirred and dispersed at 200r / min for 35min to obtain the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

[0165] The preparation method of the Centella asiatica extract includes the following steps:

[0166] Mix 10g of Centella asiatica whole herb powder with 180mL of 45% ethanol aqueous solution and extract at 35℃ for 80min using low-temperature ultrasound at 20kHz and 250W to obtain Centella asiatica extract.

[0167] Citric acid and malic acid were added to the Centella asiatica extract at a mass ratio of 2:1, with a total addition of 0.5% of the extract mass. The extract was then degraded at 45℃ and pH 3.0 for 80 minutes to obtain the degradation solution.

[0168] The degradation solution was centrifuged at 7000 r / min for 20 min, and the supernatant was collected. 1.5% of the adsorbent by mass of the degradation solution was added, and adsorption was carried out at 35℃ for 90 min. After filtration, the solution was eluted with a gradient of 20%, 50%, and 75% ethanol by volume fraction using D101 macroporous resin. The eluent was concentrated under low temperature reduced pressure at 45℃ and -0.08 MPa, and then freeze-dried at -55℃ for 28 h to obtain Centella asiatica extract.

[0169] The method for preparing the adsorbent includes the following steps:

[0170] Mix 10g of attapulgite, 5g of chitosan and 3g of food-grade activated carbon, and stir to obtain composite powder.

[0171] 10g of composite powder was mixed with 250mL of sodium bicarbonate activation solution with a mass fraction of 0.8%, and the mixture was soaked and activated at 20℃ for 35min to obtain activated composite material.

[0172] After washing the activated composite material to neutrality, it was dried at 100℃ for 2.5 hours and then pulverized through an 80-mesh sieve to obtain the self-made composite adsorbent.

[0173] The method for preparing the microencapsulated retinol includes the following steps: mixing 0.8g chitosan, 0.6g gum arabic with 80mL deionized water, and stirring at 35℃ and 200r / min for 40min to dissolve, thereby obtaining a composite wall material liquid;

[0174] 1g of retinol was added to 80mL of composite wall material solution, and the mixture was sheared at 2500r / min for 12min, and then homogenized at 50MPa for 4 times to obtain microcapsule emulsion.

[0175] Microencapsulated retinol was prepared by spray drying the microencapsulated emulsion at an inlet air temperature of 110°C and an outlet air temperature of 55°C.

[0176] Comparative Example 1

[0177] This comparative example provides a method for preparing a retinol anti-wrinkle and firming essence cream, including the following steps:

[0178] Mix 12.02g of humectant, 0.355g of thickener and stabilizer, 1.1g of preservative with 70g of deionized water, and heat and stir at 82℃ and 250r / min for 25min to obtain an aqueous phase material.

[0179] 19.55g of ethylhexyl stearate was heated to 82℃ and 180r / min for 20min to obtain the oil phase material;

[0180] The oil phase material was added to the aqueous phase material and homogenized and emulsified at 3200 r / min for 8 min. After cooling, the pH value was adjusted to 6.0~6.5 to obtain the emulsified base.

[0181] 0.175g free retinol, 0.25g Centella asiatica extract, 0.225g sophorolipid, 0.125g Aspergillus fermentation product, 0.125g type I collagen, 0.125g recombinant type III humanized collagen, and 0.0003g recombinant type XVII collagen were added to the emulsion base and dispersed by stirring at 220r / min for 30min to obtain the control sample essence cream.

[0182] The preparation method of the Centella asiatica extract includes the following steps:

[0183] Mix 10g of Centella asiatica whole herb powder with 200mL of 50% ethanol aqueous solution, and extract at 38℃ for 90min using low-temperature ultrasound at 25kHz and 300W to obtain Centella asiatica extract.

[0184] Citric acid and malic acid were added to the Centella asiatica extract at a mass ratio of 2.5:1, with a total addition of 0.65% of the extract mass. The extract was then degraded at 48℃ and pH 3.2 for 85 minutes to obtain the degradation solution.

[0185] The degradation solution was centrifuged at 8000 r / min for 15 min, and the supernatant was collected. 2.5% of the adsorbent by mass of the degradation solution was added, and adsorption was carried out at 40℃ for 70 min. After filtration, the solution was eluted with a gradient of 25%, 55%, and 80% ethanol by volume fraction using D101 macroporous resin. The eluent was concentrated under low temperature reduced pressure at 50℃ and -0.09 MPa, and then freeze-dried at -50℃ for 24 h to obtain Centella asiatica extract.

[0186] The method for preparing the adsorbent includes the following steps:

[0187] 10g of attapulgite, 6g of chitosan and 4g of food-grade activated carbon were mixed and stirred to obtain composite powder.

[0188] 10g of composite powder was mixed with 300mL of sodium bicarbonate activation solution with a mass fraction of 1.0%, and the mixture was soaked and activated at 25℃ for 30min to obtain activated composite material.

[0189] After washing the activated composite material to neutrality, it was dried at 105℃ for 2 hours, pulverized and passed through a 100-mesh sieve to obtain the self-made composite adsorbent.

[0190] Comparative Example 2

[0191] This comparative example provides a method for preparing an anti-wrinkle and firming essence cream, including the following steps:

[0192] Mix 12.02g of humectant, 0.355g of thickener and stabilizer, 1.1g of preservative with 70g of deionized water, and heat and stir at 82℃ and 250r / min for 25min to obtain an aqueous phase material.

[0193] 19.55g of ethylhexyl stearate was heated to 82℃ and 180r / min for 20min to obtain the oil phase material;

[0194] The oil phase material was added to the aqueous phase material and homogenized and emulsified at 3200 r / min for 8 min. After cooling, the pH value was adjusted to 6.0~6.5 to obtain the emulsified base.

[0195] 0.175g of microencapsulated retinol, 0.25g of Centella asiatica extract, 0.225g of sophorolipid, and 0.125g of Aspergillus fermentation product were added to the emulsion base and stirred and dispersed at 220r / min for 30min to obtain the anti-wrinkle and firming essence cream.

[0196] The preparation method of the Centella asiatica extract includes the following steps:

[0197] Mix 10g of Centella asiatica whole herb powder with 150mL of 30% ethanol aqueous solution, and extract with ultrasound at 30℃ for 60min at 15kHz and 200W to obtain Centella asiatica extract.

[0198] Citric acid was added to the Centella asiatica extract at a concentration of 0.3% of the extract mass, and the mixture was degraded at 40°C and pH 2.5 for 70 minutes to obtain the degradation solution.

[0199] The degradation solution was centrifuged at 6000 r / min for 8 min, the supernatant was collected, activated carbon was added for adsorption, and the adsorption was carried out at 30℃ for 40 min. After concentration and drying, Centella asiatica extract was obtained.

[0200] The method for preparing the adsorbent includes the following steps:

[0201] 10g of attapulgite, 6g of chitosan and 4g of food-grade activated carbon were mixed and stirred to obtain composite powder.

[0202] 10g of composite powder was mixed with 300mL of sodium bicarbonate activation solution with a mass fraction of 1.0%, and the mixture was soaked and activated at 25℃ for 30min to obtain activated composite material.

[0203] After washing the activated composite material to neutrality, it was dried at 105℃ for 2 hours, pulverized and passed through a 100-mesh sieve to obtain the self-made composite adsorbent.

[0204] The method for preparing the microencapsulated retinol includes the following steps:

[0205] Mix 1g chitosan, 0.8g gum arabic with 100mL deionized water, and stir at 40℃ and 250r / min for 30min to dissolve, thus obtaining the composite wall material liquid;

[0206] Add 1g of retinol to 100mL of composite wall material solution, shear at 3000r / min for 10min, and then homogenize at 60MPa for 3 times to obtain microcapsule emulsion.

[0207] Microencapsulated retinol was prepared by spray drying the microencapsulated emulsion at an inlet air temperature of 120°C and an outlet air temperature of 60°C.

[0208] The efficacy and safety of the serums prepared in Examples 1-3 and Comparative Examples 1-2 were verified, and the test results are shown in Table 1.

[0209] One hundred healthy subjects aged 25-55 years (including 30 with sensitive skin, 40 with mature skin, and 30 with normal skin) were randomly divided into 5 groups of 20 each. They used the essence creams prepared in Examples 1-3 and Comparative Examples 1-2, respectively, and applied them to their faces after cleansing in the morning and evening for 28 consecutive days. The laboratory samples were sealed and stored in room temperature (25°C) and high temperature (45°C) environments for 3 months for stability testing.

[0210] Skin irritation test: After 48 hours of testing, skin irritation reactions such as erythema, edema, and itching were observed. The irritation level was divided into 0-4 (0 for no irritation and 4 for severe irritation).

[0211] Wrinkle improvement rate test: The area and depth of fine lines around the eyes of the subjects were measured using a skin image analysis system after 28 days of use, and the wrinkle improvement rate was calculated.

[0212] Skin firmness improvement rate: Facial skin elasticity parameters were tested using a skin elasticity meter after 28 days of use, and the firmness improvement rate was calculated.

[0213] Barrier repair capacity test: The skin TEWL value was measured before use and 28 days after use using a transdermal moisture loss tester, and the TEWL decline rate was calculated.

[0214] Retinol stability test: The retinol content in the essence cream after 3 months of high-temperature storage was determined by high performance liquid chromatography (HPLC), and the content retention rate was calculated.

[0215] Product storage stability test: Observe the product appearance (layering, water separation, discoloration) after 3 months of storage at room temperature, and test the pH value change of the paste after 3 months of storage at high temperature (acceptable range: 5.5-6.5).

[0216] Table 1 Performance Test Results

[0217] Test Project Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Skin irritation level (48h) Level 0 Level 0 Level 0 Level 2 Level 1 Wrinkle improvement rate (28 days, %) 22.5 25.3 21.8 18.6 10.2 Firmness improvement rate (28 days, %) 19.7 23.1 18.9 15.4 8.5 TEWL decline rate (28 days, %) 35.2 38.6 33.8 27.5 16.3 Retinol content retention rate (after 3 months of high-temperature storage, %) 92.3 90.7 93.1 65.8 88.5 Appearance after 3 months of storage at room temperature Uniform and delicate, without layering Uniform and delicate, without layering Uniform and delicate, without layering Slight water separation Local stratification pH value after 3 months of high temperature storage 6.2 6.1 6.3 5.3 5.1

[0218] The technical solutions provided by the embodiments of this application have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this application. The description of the above embodiments is only for the purpose of helping to understand the method and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A retinol microcapsule anti-wrinkle and firming triple collagen essence cream, characterized in that, By weight, it consists of the following raw materials: 0.15-0.20 parts microencapsulated retinol, 0.20-0.30 parts Centella asiatica extract, 0.20-0.25 parts sophorolipid, 0.10-0.15 parts Aspergillus fermentation product, 0.10-0.15 parts type I collagen, 0.10-0.15 parts recombinant type III humanized collagen, 0.0001-0.0005 parts recombinant type XVII collagen, 12-12.5 parts humectant, 0.35-0.36 parts thickener and stabilizer, 1.0-1.2 parts preservative, 19-20 parts oil phase, and 65-75 parts deionized water.

2. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 1, characterized in that, The preparation method of the Centella asiatica extract includes the following steps: Centella asiatica whole herb powder and ethanol aqueous solution were mixed and subjected to ultrasonic extraction to obtain Centella asiatica extract. The Centella asiatica extract, citric acid, and malic acid were degraded to obtain a degradation solution; The degradation solution was subjected to centrifugation, adsorption, purification, concentration and freeze-drying in sequence to obtain the Centella asiatica extract.

3. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 2, characterized in that, The mass-to-volume ratio of the whole Centella asiatica herb powder to the ethanol aqueous solution is 1 g: (18~22) mL, and the mass fraction of the ethanol aqueous solution is 45%~55%; and / or The ultrasonic extraction process is performed at a frequency of 20kHz~30kHz, a power of 250W~350W, an extraction temperature of 35℃~40℃, and an extraction time of 80min~100min; and / or The mass ratio of citric acid to malic acid is 2-3:1, and the total amount of citric acid and malic acid added is 0.5%-0.8% of the mass of Centella asiatica extract; and / or The degradation treatment is performed at a temperature of 45℃~50℃, a system pH of 3.0~3.5, and a degradation time of 80min~90min; and / or The centrifugation process is carried out at a speed of 7000 r / min to 9000 r / min for a centrifugation time of 10 min to 20 min; and / or The adsorption treatment temperature is 35℃~45℃, the adsorption time is 50min~90min, and the amount of adsorbent added is 1.5%~3.5% of the mass of the degradation solution; and / or The purification process uses D101 type macroporous resin, with elution using a gradient of ethanol at volume fractions of 20%~30%, 50%~60%, and 75%~85%; and / or The concentration process is a low-temperature vacuum concentration, with a concentration temperature of 45℃~55℃ and a vacuum degree of -0.08MPa~-0.1MPa; and / or The freeze-drying temperature is -55℃ to -45℃, and the drying time is 20h to 28h.

4. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 2 or 3, characterized in that, The method for preparing the adsorbent used in the adsorption treatment includes the following steps: After mixing attapulgite clay, chitosan and food-grade activated carbon, the mixture is stirred to obtain a composite powder. The composite powder is mixed with the activation liquid and then subjected to an immersion activation treatment to obtain an activated composite material. The activated composite material was washed, dried, and pulverized in sequence to obtain the self-made composite adsorbent.

5. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 4, characterized in that, The mass ratio of attapulgite, chitosan, and food-grade activated carbon is 10:5~7:3~5; and / or The mass-to-volume ratio of the composite powder to the activation solution is 1 g : (25~35) mL, and the activation solution is a sodium bicarbonate aqueous solution with a mass fraction of 0.8%~1.2%; and / or The immersion activation treatment is performed at a temperature of 20℃~30℃ for 25min~35min; and / or The drying temperature is 100℃~110℃, and the time is 1.5h~2.5h; and / or The powder after pulverization has a mesh size of 80 to 120 mesh.

6. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 1, characterized in that, The method for preparing the microencapsulated retinol includes the following steps: Chitosan, gum arabic, and deionized water are mixed and stirred to dissolve, thus obtaining a composite wall material liquid. Retinol was added to the composite wall material liquid, and shearing and homogenization were performed sequentially to obtain a microcapsule emulsion. The microcapsule emulsion was spray-dried to obtain the microcapsule-encapsulated retinol.

7. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 6, characterized in that, The mass ratio of chitosan to gum arabic is (0.8~1.2):(0.6~1.0); and / or The mass-to-volume ratio of chitosan, gum arabic, and deionized water is (0.8~1.2) g : (0.6~1.0) g : (80~120) mL; and / or The stirring and dissolving process is carried out at a temperature of 35℃~45℃, a rotation speed of 200r / min~300r / min, and a time of 20min~40min; and / or The mass-to-volume ratio of retinol to the composite wall material liquid is 1g:(80~120)mL; and / or The shearing rotation speed is 2500 r / min to 3500 r / min, and the shearing time is 8 min to 12 min; and / or The homogenization process is performed at a pressure of 50 MPa to 70 MPa, and the homogenization is repeated 2 to 4 times; and / or The inlet air temperature of the spray drying process is 110℃~130℃, and the outlet air temperature is 55℃~65℃.

8. The retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 1, characterized in that, The sophorolipid is selected from one or both of lactone-type sophorolipids and acid-type sophorolipids; and / or The moisturizer is selected from one or more of glycerin, propylene glycol, sodium hyaluronate, panthenol, allantoin, or erythritol; and / or The thickening stabilizer is selected from one or both of carbomer and triethanolamine; and / or The preservative is selected from one or both of p-hydroxyacetophenone and 1,2-hexanediol; and / or The oil phase component is selected from one or more of ethylhexyl stearate, ethylhexyl palmitate, behenol, arachidonic acid, ethylhexyl hydroxystearate, or stearic acid.

9. A method for preparing the retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to any one of claims 1 to 8, characterized in that, Includes the following steps: The humectant, thickener, stabilizer, preservative and deionized water are mixed, heated and stirred to obtain an aqueous phase material. The oil phase components are heated and melted to obtain the oil phase material; The oil phase material is added to the aqueous phase material, homogenized and emulsified, and then the pH value is adjusted to 6.0~6.5 to obtain an emulsified base. The retinol microcapsule-encapsulated retinol, centella asiatica extract, sophorolipid, Aspergillus fermentation product, type I collagen, recombinant type III humanized collagen and recombinant type XVII collagen were added to the emulsion base and stirred to disperse, thus obtaining the retinol microcapsule anti-wrinkle and firming triple collagen essence cream.

10. The preparation method of the retinol microcapsule anti-wrinkle and firming triple collagen essence cream according to claim 9, characterized in that, The heating and stirring temperature is 80~85℃, the rotation speed is 200r / min~300r / min, and the time is 20min~30min; and / or The heating and melting temperature is 80~85℃, the rotation speed is 150r / min~200r / min, and the time is 15min~25min; and / or The homogenization emulsification is performed at a rotation speed of 3000 r / min to 3500 r / min for a time of 5 min to 10 min; and / or The stirring and dispersing speed is 200 r / min to 250 r / min, and the time is 25 min to 35 min.