Plant composite extract and preparation method and application thereof

Abstract

The application discloses a plant composite extract and a preparation method and application thereof. The plant composite extract comprises a first plant extract and a second plant extract with a mass ratio of (15-25):1. The first plant extract is prepared by the following method: taking clove leaf powder, scute root powder and balsam flower powder with a mass ratio of (2-5):(1-4):(1-4), mixing with water, adding cellulase, pectinase and lipase for enzymolysis to obtain an enzymolysis liquid; adding water to the enzymolysis liquid, heating and extracting, filtering, enriching active substances in the filtrate with a macroporous resin D101, then desorbing to obtain the first plant extract. The second plant extract is prepared by the following method: taking ginger root powder, adding an alcohol solution to mix, placing the mixed solution in a water bath for ultrasonic-assisted extraction, then heating and extracting, cooling, and filtering to obtain the second plant extract. The plant composite extract provided by the application can relieve scalp inflammation, reduce sebum secretion, improve dandruff problems, and has a long-lasting oil control and dandruff removal effect.

Description

Technical Field

[0001] This application belongs to the field of personal care product technology, specifically relating to a plant compound extract, its preparation method, and its application. Background Technology

[0002] In recent years, the demand for scalp health care has continued to upgrade, especially with dandruff, itching, and sensitivity becoming the most prominent scalp problems. Research has found that dandruff is closely related to the excessive proliferation of Malassezia, excessive sebum secretion, abnormal scalp keratin metabolism, damaged scalp barrier, and inflammation. Currently, most oil-control and dandruff-removing products rely on traditional chemicals such as zinc pyrithione, selenium sulfide, glycyrrhizin, piroctone olamine, clomiphene, and salicylic acid, which are highly irritating. Long-term use can damage the scalp barrier, leading to recurring scalp health problems and insufficient safety and gentleness. Plant extracts, relying on traditional Chinese medicine and its theories, and the concept of food and medicine sharing the same origin, offer advantages such as gentleness, safety, and diverse bioactivity, making them a research direction for active ingredients in personal care products. Existing plant extract products mainly inhibit Malassezia and can no longer meet the needs of the scalp care market. Summary of the Invention

[0003] To address the aforementioned problems, this application provides a plant compound extract, its preparation method, and its application. The plant compound extract provided by this application can soothe scalp inflammation, reduce scalp sebum secretion, improve dandruff problems, and has a long-lasting oil control and dandruff removal effect, which is superior to the effects of any single plant extract.

[0004] This application provides a plant compound extract, comprising a first plant extract and a second plant extract, wherein the mass ratio of the first plant extract to the second plant extract is (15~25):1. The first plant extract is prepared as follows: clove leaf powder, scutellaria root powder, and balsam powder are mixed with water, and then cellulase, pectinase, and lipase are added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; water is added to the enzymatic hydrolysate, and the mixture is heated for extraction, filtered, and the filtrate is enriched with active substances using macroporous resin D101, followed by desorption with an alcohol solution to obtain the first plant extract; wherein the mass ratio of clove leaf powder, scutellaria root powder, and balsam powder is (2~5):(1~4):(1~4). The second plant extract is prepared as follows: ginger root powder is mixed with an alcohol solution, and the mixture is placed in a water bath for ultrasonic-assisted extraction, followed by heating extraction, cooling, and filtration to obtain the second plant extract.

[0005] According to the embodiments of this application, the plant compound extract of this application, through the synergistic effect of a first plant extract obtained from raw materials composed of clove leaves, scutellaria root, and balsam flowers, and a second plant extract obtained from ginger root, forms a multi-target synergistic system. This system can significantly inhibit LPS-induced inflammatory responses at the cellular level and significantly reduce TH. This invention modulates the secretion level of the inflammatory mediator PGE2 in cells and effectively reduces the excessive production of nitric oxide (NO) in RAW264.7 macrophages, thus blocking the scalp inflammation signaling pathway at its source and achieving scalp soothing and anti-inflammatory protection. Simultaneously, human testing data shows that this plant compound extract can significantly reduce scalp sebum secretion and dandruff production, thus possessing a triple effect of soothing and anti-inflammatory, oil control, and dandruff removal. Compared with single plant extracts, the plant compound extract of this application has higher efficacy and more significant effects. Furthermore, the plant compound extract of this application employs a combined process of enzymatic hydrolysis, macroporous resin purification, and ultrasound-assisted extraction, which can significantly improve the dissolution and purity of active ingredients. The extract has good stability, no cytotoxicity, and can be safely applied to various personal care products.

[0006] In some alternative embodiments, clove leaves are from the Oleaceae family ( Oleaeeae )clove( Syringa oblata The leaves of Scutellaria baicalensis, and the root of Scutellaria baicalensis (of the Lamiaceae family) Scutellaria baicalensis Georgi The root of the balsam plant (Impatiens balsamina) belongs to the Balsaminaceae family. Impatiens balsamina L. (The flower.)

[0007] Secondly, an embodiment of this application provides a method for preparing a plant compound extract, comprising: Preparation of the first plant extract: Take clove leaf powder, scutellaria root powder and balsam powder, mix with water, add cellulase, pectinase and lipase for enzymatic hydrolysis to obtain enzymatic hydrolysate; add water to the enzymatic hydrolysate, heat for extraction, filter, enrich the active substances in the filtrate with macroporous resin D101, and then add alcohol solution for desorption to obtain the first plant extract; wherein, the mass ratio of clove leaf powder, scutellaria root powder and balsam powder is (2~5):(1~4):(1~4).

[0008] Preparation of the second plant extract: Take ginger root powder, add alcohol solution and mix. Place the mixture in a water bath for ultrasonic-assisted extraction, then heat extraction, cool down, filter and obtain the second plant extract. Preparation of plant compound extract: Mix the first plant extract and the second plant extract at a mass ratio of (15~25):1, filter, and obtain the plant compound extract.

[0009] According to the embodiments of this application, the method provided by this application is controllable and reproducible. The prepared plant compound extract is clear and transparent, mild and non-cytotoxic, and can be stably used in various soothing, anti-inflammatory, oil-controlling and dandruff-removing personal care products, solving the defects of traditional extraction processes such as low activity, poor purity and single efficacy.

[0010] In some optional embodiments, in the step of preparing the first plant extract, the mass ratio of cellulase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.01 to 0.02. The mass ratio of pectinase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.001 to 0.002. The mass ratio of lipase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.01 to 0.02.

[0011] In some alternative embodiments, in the step of preparing the first plant extract, the mass of water added each time is 5 to 10 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0012] In some optional embodiments, in the step of preparing the first plant extract, the mass of macroporous resin D101 is 2 to 4 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder; the alcohol solution is selected from any one of an aqueous solution of 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol, or butanediol with a mass percentage of 70% to 90%; the mass of the alcohol solution is 10 to 12 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0013] In some optional embodiments, in the step of preparing the second plant extract, the alcohol solution is selected from any one of an aqueous solution of 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol or butanediol with a mass percentage of 70% to 90%; the mass of the alcohol solution is 5 to 8 times the mass of the ginger root powder.

[0014] Thirdly, the embodiments of this application provide the application of the plant compound extract of the first aspect or the plant compound extract prepared by the preparation method of the second aspect in the preparation of personal care products with soothing and anti-inflammatory, oil-controlling or dandruff-removing effects.

[0015] Fourthly, according to an embodiment of this application, a personal care product includes a plant compound extract from the first aspect or a plant compound extract prepared by the preparation method of the second aspect.

[0016] In some optional embodiments, the personal care product is selected from any one of toners, serums, creams, lotions, cleansers, and scalp care products; wherein the plant compound extract has a mass percentage of 0.01% to 5% in the personal care product. This addition amount is mild and non-cytotoxic, has good compatibility with various cosmetic formulation systems, does not affect the product's appearance, viscosity, stability, or user experience, and can be widely adapted to all categories of skincare and scalp care products; it can ensure clinically visible oil control, dandruff removal, and soothing improvement effects while meeting cosmetic safety regulations, possessing strong industrial feasibility and market application value. Attached Figure Description

[0017] Figure 1 This figure shows the effect of the plant compound extract of Example 1 and the extracts of Comparative Examples 1 and 2 on the expression level of PGE2 in THP-1 cells. The results are expressed as Mean ± SEM. Data were analyzed using t-test. ###p<0.001 indicates a highly significant difference between the NC group and the BC group; *p<0.05 indicates a significant difference between the sample group and the NC group; **p<0.01, ***p<0.001 indicate a highly significant difference between the sample group and the NC group.

[0018] Figure 2 This figure shows the effect of the plant compound extract from Example 2 and the extracts from Comparative Examples 3 and 4 on the expression level of PGE2 in THP-1 cells. The results are expressed as Mean ± SEM. Data were analyzed using t-test. ###p<0.001 indicates a highly significant difference between the NC group and the BC group; **p<0.01, ***p<0.001, and ****p<0.0001 indicate a highly significant difference between the sample groups and the NC group.

[0019] Figure 3 This figure shows the effect of the plant compound extract of Example 3 and the extracts of Comparative Examples 5 to 8 on the expression level of PGE2 in THP-1 cells. The results are expressed as Mean ± SEM. Data were analyzed using t-test, where ns indicates no significant difference, ###p<0.001 indicates a highly significant difference between the NC group and the BC group, and **p<0.01 and ***p<0.001 indicate a highly significant difference between the sample groups and the NC group.

[0020] Figure 4 This figure shows the effect of the plant compound extract of Example 1 and the extracts of Comparative Examples 1 and 2 on NO production by RAW264.7 macrophages. Results are expressed as Mean ± SEM. Data were obtained using... t-test statistical analysis, where #####p<0.0001 indicates a highly significant difference between the NC group and the BC group; *p<0.05 indicates a significant difference between the sample group and the NC group; and **p<0.01 indicates a highly significant difference between the sample group and the NC group.

[0021] Figure 5 This figure shows the effect of the plant compound extract from Example 2 and the extracts from Comparative Examples 3 and 4 on NO production by RAW264.7 macrophages. Results are presented as mean ± SEM. Data were obtained using... t - Statistical analysis was performed, where #####p<0.0001 indicates a highly significant difference between the NC group and the BC group; **p<0.01 and ***p<0.001 indicate a highly significant difference between the sample group and the NC group.

[0022] Figure 6 This is a graph showing the effect of the plant compound extract of Example 3 and the extracts of Comparative Examples 5 to 8 on NO production by RAW264.7 macrophages. Results are expressed as Mean ± SEM. Data were obtained using... t -test statistical analysis, where ns indicates no significant difference, ####p<0.0001 indicates a highly significant difference between the NC group and the BC group; **p<0.01, ***p<0.001 indicate a highly significant difference between the sample group and the NC group.

[0023] Figure 7 The results of the irritation test of the plant compound extract on the chorioallantoic membrane of chicken embryo in Example 3 are shown in the figure.

[0024] Figure 8 This is a dandruff rating chart.

[0025] Figure 9 This is a graph showing the results of oil content at various time points after using the scalp care essence and a blank sample.

[0026] Figure 10 This is a graph showing the rate of change in skin oil levels at various time points after using the scalp care essence for testing and a blank sample.

[0027] Figure 11 This is a statistical chart of dandruff severity scores at various time points after using the scalp care essence for testing and a blank sample.

[0028] Figure 12 This is a graph showing the rate of change in dandruff scores at various time points after using the scalp care essence for testing and a blank sample.

[0029] Figure 13 This is a comparison of scalp photos taken at various time points after using the scalp care essence for testing and a blank sample. Figure 13 The photo on the left shows the scalp care serum used for testing. Figure 13 The photo on the right is a blank sample. Detailed Implementation

[0030] To make the purpose, technical solution, and beneficial technical effects of this application clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the embodiments described in this specification are merely for explaining this application and are not intended to limit it.

[0031] For simplicity, this application only explicitly discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form a range not explicitly stated; and any lower limit can be combined with other lower limits to form a range not explicitly stated, just as any upper limit can be combined with any other upper limit to form a range not explicitly stated. Furthermore, although not explicitly stated, every point or individual value between the endpoints of the range is included within that range. Therefore, each point or individual value can be used as its own lower or upper limit and combined with any other point or individual value or with other lower or upper limits to form a range not explicitly stated.

[0032] In the description of this application, it should be noted that, unless otherwise stated, "above" and "below" include the stated number, and "multiple" in "one or more" means two or more.

[0033] The foregoing description of this application is not intended to describe every disclosed implementation or method. Instead, the following description provides more specific examples of exemplary embodiments. Throughout the application, guidance is provided through a series of embodiments that can be used in various combinations. The examples listed are representative only and should not be construed as exhaustive.

[0034] Based on the problems mentioned in the background art, the inventors, through in-depth research and extensive experiments, have provided a plant compound extract, its preparation method, and its application. The plant compound extract provided in this application can soothe scalp inflammation, reduce scalp oil secretion, improve dandruff problems, and has a long-lasting oil control and dandruff removal effect, which is superior to the effects of any single plant extract.

[0035] The plant compound extract of this application, its preparation method, and its application will be described below with reference to the embodiments of this application.

[0036] Plant compound extract This application provides a plant compound extract, which includes a first plant extract and a second plant extract, wherein the mass ratio of the first plant extract to the second plant extract is (15~25):1.

[0037] The first plant extract was prepared by the following method: clove leaf powder, scutellaria root powder and balsam powder were mixed with water, and cellulase, pectinase and lipase were added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; water was added to the enzymatic hydrolysate, and the mixture was heated for extraction, filtered, and the active substances in the filtrate were enriched with macroporous resin D101. Then, an alcohol solution was added for desorption to obtain the first plant extract; wherein the mass ratio of clove leaf powder, scutellaria root powder and balsam powder was (2~5):(1~4):(1~4).

[0038] The second plant extract was prepared by the following method: ginger root powder was mixed with an alcohol solution, the mixture was placed in a water bath for ultrasonic-assisted extraction, then heated for extraction, cooled, and filtered to obtain the second plant extract.

[0039] As an example, the mass ratio of the first plant extract to the second plant extract can be 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1 or any range of the above values.

[0040] As an example, the mass ratio of clove leaf powder, scutellaria root powder, and balsam powder can be 2:1:1, 5:4:4, 2:2:2, 2:1:4, 5:2:2, 5:1:4, or any range of the above values.

[0041] According to the embodiments of this application, the plant compound extract of this application, through the synergistic effect of a first plant extract obtained from raw materials composed of clove leaves, scutellaria root, and balsam flowers, and a second plant extract obtained from ginger root, forms a multi-target synergistic system. This system can significantly inhibit LPS-induced inflammatory responses at the cellular level and significantly reduce TH. This invention modulates the secretion level of the inflammatory mediator PGE2 in cells and effectively reduces the excessive production of nitric oxide (NO) in RAW264.7 macrophages, thus blocking the scalp inflammation signaling pathway at its source and achieving scalp soothing and anti-inflammatory protection. Simultaneously, human testing data shows that this plant compound extract can significantly reduce scalp sebum secretion and dandruff production, thus possessing triple effects of anti-inflammatory soothing, oil control, and dandruff removal. Compared with single plant extracts, the plant compound extract of this application has higher efficacy and more significant effects. Furthermore, the plant compound extract of this application employs a combined process of enzymatic hydrolysis, macroporous resin purification, and ultrasound-assisted extraction, which can significantly improve the dissolution and purity of active ingredients. The extract has good stability, no cytotoxicity, and can be safely applied to various personal care products.

[0042] In some embodiments, clove leaves are from the Oleaceae family ( Oleaeeae )clove( Syringa oblata The leaves of Scutellaria baicalensis, and the root of Scutellaria baicalensis (from the Lamiaceae family). Scutellaria baicalensis Georgi The dried root of *Impatiens balsamina*, a plant in the Balsamicaceae family. Impatiens balsamina L. (Dried flowers)

[0043] Preparation method of plant compound extract This application provides a method for preparing a plant compound extract, comprising: To prepare the first plant extract, clove leaf powder, scutellaria root powder, and balsam powder were mixed with water, and cellulase, pectinase, and lipase were added for enzymatic hydrolysis to obtain the hydrolysate. Water was added to the hydrolysate, and the mixture was heated for extraction. The mixture was filtered, and the active substances in the filtrate were enriched with macroporous resin D101. Then, an alcohol solution was added for desorption to obtain the first plant extract. The mass ratio of clove leaf powder, scutellaria root powder, and balsam powder was (2~5):(1~4):(1~4).

[0044] To prepare the second plant extract, ginger root powder was mixed with an alcohol solution, and the mixture was placed in a water bath for ultrasonic-assisted extraction. Then, it was heated for extraction, cooled, and filtered to obtain the second plant extract. To prepare a plant compound extract, the first plant extract and the second plant extract were mixed at a mass ratio of (15~25):1, filtered, and the resulting plant compound extract was obtained.

[0045] As an example, the mass ratio of the first plant extract to the second plant extract can be 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1 or any range of the above values.

[0046] As an example, the mass ratio of clove leaf powder, scutellaria root powder, and balsam powder can be 2:1:1, 5:4:4, 2:2:2, 2:1:4, 5:2:2, 5:1:4, or any range of the above values.

[0047] According to the embodiments of this application, the first plant extract employs a three-step enzymatic hydrolysis using cellulase, pectinase, and lipase to efficiently disrupt plant cell walls and significantly increase the dissolution rate of soothing, antibacterial, and oil-controlling active ingredients in clove leaves, scutellaria root, and impatiens. Combined with heating extraction and enrichment purification using D101 macroporous resin, impurities are effectively removed, and active substances are enriched, improving the purity and stability of the extract. The second plant extract uses a two-stage extraction method combining alcohol solution water bath ultrasonic-assisted extraction and heating extraction to fully release the soothing and antibacterial active ingredients in ginger root, achieving high extraction efficiency and mild conditions. After being compounded at a specific mass ratio, the composite extract can significantly inhibit LPS-induced TH at the cellular level. Excessive release of PGE2 from cells and NO production in RAW264.7 macrophages reduce scalp inflammation at its source, while achieving synergistic effects of scalp oil control and dandruff removal.

[0048] The method and process provided in this application are controllable and reproducible. The prepared extract is clear, transparent, mild and non-cytotoxic, and can be stably used in various soothing, oil-controlling and dandruff-removing personal care products, thus solving the defects of traditional extraction processes such as low activity, poor purity and single efficacy.

[0049] In some embodiments, in the step of preparing the first plant extract, the mass ratio of cellulase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.01 to 0.02. The mass ratio of pectinase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.001 to 0.002. The mass ratio of lipase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder is 0.01 to 0.02.

[0050] As an example, the ratio of the mass of cellulase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder can be 0.01, 0.012, 0.014, 0.016, 0.018, 0.02, or any of the above values.

[0051] As an example, the ratio of the mass of pectinase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder can be 0.001, 0.0012, 0.0014, 0.0016, 0.0018, 0.002, or any of the above values.

[0052] As an example, the ratio of the mass of lipase to the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder can be 0.01, 0.012, 0.014, 0.016, 0.018, 0.02, or any of the above values.

[0053] In the above embodiments, when the amounts of cellulase, pectinase, and lipase meet the requirements of this application, a synergistic, efficient, mild, and controllable complex enzymatic hydrolysis system can be formed. This system can precisely disrupt the cell walls and interstitial structures of clove leaves, scutellaria root, and impatiens, significantly improving the dissolution efficiency and yield of anti-inflammatory, antibacterial, and oil-controlling active ingredients such as flavonoids, polyphenols, and volatile oils. This reduces both incomplete cell wall disruption and insufficient release of active ingredients caused by insufficient enzyme dosage, and excessive enzyme dosage leading to degradation of active substances, increased system impurities, and increased pressure on subsequent purification. After optimized enzymatic hydrolysis, the purity and stability of the active ingredients in the first plant extract are higher.

[0054] In some embodiments, in the step of preparing the first plant extract, the mass of water added each time is 5 to 10 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder. This allows the clove leaf, scutellaria root, and balsam powder to be fully soaked and dispersed, improving the solid-liquid contact and mass transfer efficiency during enzymatic hydrolysis and heating extraction. This ensures thorough enzymatic hydrolysis of plant cell walls and complete dissolution of active ingredients, while reducing the risk of excessive dilution of active ingredients due to excessive solvent volume, and avoiding increased energy consumption and cost in subsequent purification.

[0055] As an example, the mass of water added each time can be 5, 6, 7, 8, 9, 10 times, or any combination of the above values, of the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0056] In some embodiments, in the step of preparing the first plant extract, the mass of macroporous resin D101 is 2 to 4 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder; the alcohol solution is selected from any one of an aqueous solution of 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol, or butanediol with a mass percentage of 70% to 90%; the mass of the alcohol solution is 10 to 12 times the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0057] As an example, the mass of macroporous resin D101 is 2, 2.5, 3, 3.5, 4 times, or any combination of the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0058] As an example, the mass percentage of alcohol in the alcohol solution can be 70%, 75%, 80%, 85%, 90%, or any of the above values.

[0059] As an example, the mass of the alcohol solution is 10, 10.5, 11, 11.5, 12 times, or any combination of the above values, of the sum of the masses of clove leaf powder, scutellaria root powder, and balsam powder.

[0060] In the above embodiments, when the step of preparing the first plant extract meets the requirements of this application, the macroporous resin can efficiently enrich flavonoids, polyphenols, terpenes, and other core active substances with anti-inflammatory, antibacterial, and oil-controlling properties, while effectively removing large molecular impurities such as polysaccharides, colloids, and pigments. This significantly improves the purity, clarity, and storage stability of the extract, reducing problems such as turbidity, irritation, or instability in formulation caused by impurities. Simultaneously, the alcoholysis adsorption system, under mild conditions, directionally and completely desorbs the enriched active ingredients, maximizing the preservation of the active substance's structure and biological activity without causing efficacy loss.

[0061] In some optional embodiments, in the step of preparing the second plant extract, the alcohol solution is selected from any one of the following aqueous solutions with a mass percentage of 70% to 90%: 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol, or butylene glycol; the mass of the alcohol solution is 5 to 8 times the mass of the ginger root powder. This extraction system and solid-liquid ratio can maximize the targeted extraction of volatile oils, gingerol, polyphenols, and other soothing, oil-controlling, and antibacterial active ingredients from ginger root, while reducing the dissolution of water-soluble impurities and starchy substances, thus improving the purity and stability of the extract.

[0062] As an example, the mass percentage of alcohol in the alcohol solution can be 70%, 75%, 80%, 85%, 90%, or any of the above values.

[0063] As an example, the mass of the alcohol solution can be 5 times, 5.5 times, 6 times, 6.5 times, 7 times, 7.5 times, 8 times, or any combination of the above values, of the mass of the ginger root powder.

[0064] This application provides an application of the above-mentioned plant compound extract or the plant compound extract prepared by the above preparation method in the preparation of personal care products with soothing, anti-inflammatory, oil-controlling or dandruff-removing effects.

[0065] Personal care products This application provides a personal care product, including a plant compound extract or a plant compound extract prepared by the above preparation method.

[0066] In some embodiments, the personal care product is selected from any one of toner, serum, cream, lotion, facial cleanser, and scalp care product; wherein the plant compound extract has a mass percentage content of 0.01% to 5% in the personal care product.

[0067] As an example, the percentage of plant compound extracts in personal care products can be 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, or any combination of the above values.

[0068] In the above embodiments, when the plant compound extract contains 0.01% to 5% by mass in the personal care product, the plant compound extract in the personal care product can fully exert the synergistic effect of multiple plants and significantly inhibit LPS-induced TH at the cellular level. Excessive secretion of PGE2 by cells and massive production of NO by RAW264.7 macrophages continuously soothe scalp inflammation. Simultaneously, it stably achieves the triple effects of oil control, dandruff removal, and soothing. Low dosage is sufficient to achieve ideal results, avoiding the risk of irritation from excessive concentrations and the insignificant efficacy from insufficient concentrations.

[0069] Meanwhile, this addition is mild and non-cytotoxic, has good compatibility with various cosmetic formulation systems, and does not affect the product's appearance, viscosity, stability, or user experience. It can be widely adapted to all categories of skincare and scalp care products. It can ensure clinically visible oil control, dandruff removal, and soothing improvement effects while meeting cosmetic safety regulations, and has strong industrial feasibility and market application value.

[0070] Example The following embodiments describe the contents of this invention in more detail. These embodiments are merely illustrative, as various modifications and variations will be apparent to those skilled in the art within the scope of this disclosure. Unless otherwise stated, all parts, percentages, and ratios reported in the following embodiments are based on mass, and all reagents used in the embodiments are commercially available or synthesized by conventional methods and can be used directly without further processing. The instruments used in the embodiments are also commercially available. In the embodiments and comparative examples of this application, clove leaves were purchased from Anguo Yaoyuan Trading Co., Ltd.; Scutellaria baicalensis root was purchased from Anhui Kangweifu Co., Ltd.; Impatiens balsamina was purchased from Anhui Kangweifu Co., Ltd.; and ginger root was purchased from Anhui Kangweifu Co., Ltd.

[0071] Example 1 A plant compound extract is prepared by the following method: Preparation of the first plant extract: Take 50g of clove leaf powder, 25g of Scutellaria baicalensis root powder, and 25g of Impatiens balsamina powder, add 500g of water and mix. Then add 2g of cellulase, 0.2g of pectinase, and 2g of lipase. After enzymatic hydrolysis at 45℃ for 1h, add 1000g of water, raise the temperature to 90℃, and extract for 3h. Filter and collect the filtrate and clarify it. Add 400g of macroporous resin D101 and adsorb at room temperature for 2h. Then collect the D101 resin and add 1200g of 75% butanediol aqueous solution for desorption. Keep warm and stir at 65℃ for 2h. After sedimentation, filter with a 5-1μm microfiltration membrane to obtain a deep yellow, clear, and transparent liquid, which is the first plant extract.

[0072] Preparation of the second plant extract: Take 10g of ginger root powder, add 80g of 75% butanediol aqueous solution, stir evenly, place the mixture in a 50℃ water bath, and extract with ultrasonic assistance for 1h. Then, perform a second extraction on the mixture, place the mixture in a 90℃ environment, and extract with constant temperature stirring for 1h. Cool down and filter to remove the residue, collect the liquid, and filter it with a 5-0.1μm microfiltration membrane to obtain a yellow filtrate, which is the second plant extract.

[0073] Preparation of plant compound extract: The first plant extract and the second plant extract prepared above are mixed at a mass ratio of 15:1, stirred evenly, and filtered twice through a 0.45μm ceramic membrane to obtain a deep yellow, clear and transparent liquid, which is the plant compound extract.

[0074] Example 2 A plant compound extract is prepared by the following method: Preparation of the first plant extract: Take 50g of clove leaf powder, 40g of Scutellaria baicalensis root powder, and 40g of Impatiens balsamina powder, add 900g of water and mix. Then add 1.3g of cellulase, 0.13g of pectinase, and 1.3g of lipase. Enzymatically hydrolyze at 45℃ for 1h. Add 1300g of water, raise the temperature to 90℃, and extract for 3h. Filter, collect the filtrate and clarify it. Add 360g of macroporous resin D101 and adsorb at room temperature for 2h. Collect the D101 resin and add 1300g of 80% hexanediol aqueous solution for desorption. Keep warm and stir at 65℃ for 2h. After sedimentation, filter with a 5-0.1μm microfiltration membrane to obtain a deep yellow, clear, and transparent liquid, which is the first plant extract.

[0075] Preparation of the second plant extract: Take 10g of ginger root powder, add 52g of 80% hexanediol aqueous solution, stir evenly, place the mixture in a 50℃ water bath, and extract with ultrasonic assistance for 1h. Then, perform a second extraction on the mixture, place the mixture in a 90℃ environment, and extract with constant temperature stirring for 1h. Cool down and filter to remove the residue, collect the liquid, and filter it with a 5μm-0.1μm microfiltration membrane to obtain a yellow filtrate, which is the second plant extract.

[0076] Preparation of plant compound extract: The first plant extract and the second plant extract prepared above are mixed at a mass ratio of 25:1, stirred evenly, and filtered twice through a 0.45μm ceramic membrane to obtain a deep yellow, clear and transparent liquid, which is the plant compound extract.

[0077] Example 3 A plant compound extract is prepared by the following method: Preparation of the first plant extract: Take 45g of clove leaf powder, 30g of Scutellaria baicalensis root powder, and 25g of Impatiens balsamina powder, add 800g of water and mix. Then add 1.5g of cellulase, 0.15g of pectinase, and 1.5g of lipase. Enzymatically hydrolyze at 45℃ for 1.5h. Add 1000g of water, raise the temperature to 90℃, and extract for 3h. Filter and collect the filtrate and clarify it. Add 200g of macroporous resin D101 and adsorb at room temperature for 2h. Collect the D101 resin and add 1000g of 85% 1,3-propanediol aqueous solution for desorption at 65℃. Keep warm and stir for 2h. After sedimentation, filter with a 5-0.1μm microfiltration membrane to obtain a deep yellow, clear, and transparent liquid, which is the first plant extract.

[0078] Preparation of the second plant extract: Take 10g of ginger root powder, add 50g of 85% 1,3-propanediol aqueous solution, stir evenly, place the mixture in a 50℃ water bath, and extract with ultrasonic assistance for 1h. Then, perform a second extraction on the mixture, place the mixture in a 90℃ environment, and extract with constant temperature stirring for 1h. Cool down and filter to remove the residue, collect the liquid, and filter with a 5-0.1μm microfiltration membrane to obtain a yellow filtrate, which is the second extract.

[0079] Preparation of plant compound extract: The first plant extract and the second plant extract prepared above are mixed at a mass ratio of 20:1, stirred evenly, and filtered twice through a 0.45μm ceramic membrane to obtain a deep yellow, clear and transparent liquid, which is the plant compound extract.

[0080] Comparative Example 1 The second plant extract (i.e. ginger root extract) prepared in Example 1 was used as the extract for Comparative Example 1, as detailed in Table 1.

[0081] Comparative Example 2 The first plant extract prepared in Example 1 (i.e., extracts of clove leaves, scutellaria root, and balsam flowers) was used as the extract for Comparative Example 2, as detailed in Table 1.

[0082] Comparative Example 3 The second plant extract (i.e. ginger root extract) prepared in Example 2 was used as the extract for Comparative Example 3, as detailed in Table 1.

[0083] Comparative Example 4 The first plant extract prepared in Example 2 (i.e., extracts of clove leaves, scutellaria root, and balsam flowers) was used as the extract for Comparative Example 4, as detailed in Table 1.

[0084] Comparative Example 5 The second plant extract (i.e. ginger root extract) prepared in Example 3 was used as the extract for Comparative Example 5, as detailed in Table 1.

[0085] Comparative Example 6 The first plant extract prepared in Example 3 (i.e., extracts of clove leaves, scutellaria root, and balsam flowers) was used as the extract for Comparative Example 6, as detailed in Table 1.

[0086] Comparative Example 7 A compound plant extract is prepared by referring to the method of Example 3 to prepare the first extract and the second extract. In the preparation process, the ratio of the amount of extraction solvent, enzyme, macroporous resin, etc. to the mass of the extraction raw materials is the same as in Example 3. The only difference from Example 3 is that the mass ratio of the first plant extract and the second plant extract in the compound plant extract is 1:1. See Table 1 for details.

[0087] Comparative Example 8 A compound plant extract is prepared by referring to the method of Example 3 to prepare the first extract and the second extract. In the preparation process, the ratio of the amount of extraction solvent, enzyme, macroporous resin, etc. to the mass of the extraction raw materials is the same as in Example 3. The only difference from Example 3 is that the mass ratio of the first plant extract and the second plant extract in the compound plant extract is 30:1. See Table 1 for details.

[0088] Table 1. Process parameters for Examples 1 to 3 and Comparative Examples 1 to 8

[0089] Note: In Table 1, “ / ” indicates that no corresponding substance was added during the preparation of the extract, that is, the amount of the corresponding substance used was 0 or there was no corresponding proportion.

[0090] Detection Example 1 The effects of the extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 8 on the expression level of PGE2 in THP-1 cell culture medium were tested, including the following steps: 1) Cell seeding: After cell resuscitation, when the confluence reaches approximately 80%, seed the cells at a rate of 8 × 10⁶ cells / year. 4 Cells were seeded into 24-well plates and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0091] 2) Experimental Groups: The experiment included a blank control group, a negative control group, and a sample group. In the sample group, the samples were diluted solutions obtained by diluting the extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 8 with pure water. The mass concentration of each extract in the sample was 0.2%, and 3 replicate wells were set up.

[0092] 3) Solution preparation: Prepare working solutions of different concentrations according to Table 2.

[0093] Table 2 Grouping of PGE2 Expression Level Tests in THP-1 Cell Culture Medium

[0094] 4) Drug administration: After 24 hours of incubation, administer the drug. For the blank control group, add 500 μL of complete culture medium (89% DMEM, 10% premium fetal bovine serum, 1% penicillin-streptomycin) to each well; for the negative control group, add 500 μL of complete culture medium containing 2 μg / mL LPS to each well; for the sample group, add 500 μL of complete culture medium containing 2 μg / mL LPS and the sample to be tested to each well. After drug administration, incubate the 24-well plate in a CO2 incubator (37℃, 5% CO2) for 24 hours.

[0095] 5) After the incubation period, collect the supernatant; centrifuge at 12,000 rpm for 10 min, and determine the PGE2 content according to the ELISA kit instructions.

[0096] The graph was plotted using GraghPad Prism, and the results are as follows: Figures 1 to 3 As shown, the plant compound extracts prepared in Examples 1 to 3 (the first plant extract extracted from clove leaves, scutellaria root, and balsam flowers and the second plant extract extracted from ginger root, mixed at a ratio of (15~25):1) can significantly reduce the secretion of PEG2 in THP-1 cells. This effect is superior to the combined effect of using the first plant extract (the first plant extract extracted from clove leaves, scutellaria root, and balsam flowers) and the second plant extract (the second plant extract extracted from ginger root) alone.

[0097] Detection Example 2 The effects of the extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 8 on the NO production of RAW264.7 macrophages were tested, including the following steps: 1) After cell seeding and resuscitation, when the confluence reaches approximately 80%, use 8 × 10⁶ cells / year. 4 Cells were seeded into 24-well plates and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0098] 2) Experimental grouping: The experiment included a blank control group, a negative control group, and a sample group. In the sample group, the samples were diluted solutions obtained by diluting the extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 8 with pure water. The mass concentration of each extract in the sample was 0.2%, and 3 replicate wells were set up.

[0099] 3) Prepare working solutions: Prepare working solutions of different concentrations according to Table 3.

[0100] Table 3. Grouping Table for RAW264.7 Macrophage NO Production Test

[0101] 4) Drug administration: After 24 hours of incubation, administer the drug. For the blank control group, add 500 μL of complete culture medium (89% DMEM, 10% premium fetal bovine serum, 1% penicillin-streptomycin) to each well; for the negative control group, add 500 μL of complete culture medium containing 2 μg / mL LPS to each well; for the sample group, add 500 μL of complete culture medium containing 2 μg / mL LPS and the sample to be tested to each well. After drug administration, incubate the 24-well plate in a CO2 incubator (37℃, 5% CO2) for 24 hours.

[0102] 5) After the incubation period, collect the supernatant; centrifuge at 12,000 rpm for 10 min, and determine the NO content according to the Griess Reagent System.

[0103] The graph was plotted using GraghPad Prism, and the results are as follows: Figures 4 to 6 As shown. The plant compound extracts prepared in Examples 1 to 3 (the first plant extract extracted from clove leaves, scutellaria root, and balsam flowers and the second plant extract extracted from ginger root, mixed at a ratio of (15~25):1) can significantly reduce NO secretion in RAW264.7 macrophages. This efficacy is superior to the combined effects of using the first plant extract (the first plant extract extracted from clove leaves, scutellaria root, and balsam flowers) and the second plant extract (the second plant extract extracted from ginger root) alone.

[0104] As known from Test Examples 1 and 2, the plant compound extracts prepared in Examples 1 to 3 have a synergistic effect, achieving remarkably significant anti-inflammatory and soothing effects even at low application concentrations. Excessive oil production and dandruff on the scalp or skin are often closely related to local micro-inflammatory states. Studies have shown that inflammatory factors can stimulate sebaceous gland cell proliferation and sebum synthesis, while simultaneously inducing excessive proliferation or abnormal shedding of keratinocytes, forming visible flakes. Therefore, by inhibiting the release of inflammatory mediators and soothing the skin's stress state, the problems of excessive sebum secretion and abnormal dandruff can be improved simultaneously from the pathophysiological source. Test Examples 1 and 2 have confirmed at the cellular level that the plant compound extracts prepared in this application have significant synergistic anti-inflammatory and soothing effects, which can reduce the pathological stimulation of sebaceous glands and keratinocytes from the source, thus providing a basis for achieving oil control and dandruff removal effects. Test Examples 3 and 4 use the plant compound extract prepared in Example 3 as an example to test its irritation to humans and its dandruff removal and oil control effects.

[0105] Detection Example 3 The irritation of the plant compound extract was evaluated using chicken embryo chorioallantoic membrane. Specific testing procedures followed the group standard T / SHRH 011-2018 "Cosmetic Eye Irritation Test: Chicken Embryo Chollioallantoic Membrane Test". The test sample was a diluted solution obtained by diluting the plant compound extract prepared in Example 3 with pure water. The mass concentration of the plant compound extract in the test sample was 5%, and three parallel experiments were performed.

[0106] See results Figure 7 After adding 0.1 mol / L NaOH for 5 minutes, significant vascular dissolution occurred in the chorioallantoic membrane of chicken embryos, indicating strong irritation. After adding 0.9% NaCl for 5 minutes, no vascular dissolution occurred in the chorioallantoic membrane of chicken embryos. After adding 5% of the plant compound extract prepared in Example 3 for 5 minutes, no vascular dissolution occurred in the chorioallantoic membrane of chicken embryos, and there was no irritation. This indicates that the plant compound extract prepared in Example 3 is non-irritating and safe for human use at a concentration of 5%.

[0107] Detection Example 4 The plant compound extract prepared in Example 3 was added to the blank matrix of scalp care essence at a concentration of 5%. The pH was adjusted to 5.5-6.5 using triethanolamine to obtain the scalp care essence for testing. The specific formula is detailed in Table 4.

[0108] Table 4. Ingredients of Scalp Care Essence

[0109] Clinical trials were conducted to evaluate the dandruff-reducing and oil-controlling effects of the aforementioned scalp care serums, referring to the dandruff scoring levels in the group standard "TZHCA019-2022 Test Method for Anti-dandruff Efficacy of Cosmetics". Figure 8 Five volunteers with a dandruff rating of level 4 were selected from 20 volunteers to participate in this test. A blank sample was also included in the test; the only difference between the blank sample and the test scalp care essence was that the blank sample did not contain plant compound extracts. The specific testing method is as follows: Apply the test scalp care essence (or blank sample) to the scalp and hairline, gently massage until absorbed, divide the scalp into two parts along the midline, apply the blank sample to the left side and the test scalp care essence to the right side, twice daily. The dandruff-reducing and oil-controlling effects were monitored using the VC 20 skin analysis system (Visioscan® VC 20plus) and the Sebumeter® SM815 device for a two-week period.

[0110] The test results of the 5 volunteers were basically consistent, with the test results of 2 volunteers being as follows: Figures 9-13 As shown.

[0111] like Figure 9 and Figure 10As shown, on the side using the scalp care essence for testing, scalp oil decreased by 29.02% after 7 days; after 14 days, scalp oil decreased by 47.05%, with significantly better oil improvement than the side using the blank sample. This indicates that the plant compound extract of this application has a good oil-controlling effect.

[0112] like Figure 11 , Figure 12 and Figure 13 As shown, on the side using the scalp care essence for testing, the scalp and dandruff score decreased by 38.9% after 7 days; after 14 days, the scalp and dandruff score decreased by 61.1%, which was 41% lower than the side using the blank sample. The significant decrease in dandruff and dandruff score indicates that the plant compound extract of this application has a good effect on improving dandruff.

[0113] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A plant compound extract, characterized in that, include: The first plant extract and the second plant extract have a mass ratio of (15~25):

1. The first plant extract is prepared by the following method: clove leaf powder, scutellaria root powder, and balsam powder are mixed with water, and cellulase, pectinase, and lipase are added for enzymatic hydrolysis to obtain an enzymatic hydrolysate; water is added to the enzymatic hydrolysate, and the mixture is heated for extraction, filtered, and the filtrate is enriched with macroporous resin D101 to collect active substances. Then, an alcohol solution is added for desorption to obtain the first plant extract; wherein the mass ratio of the clove leaf powder, the scutellaria root powder, and the balsam powder is (2~5):(1~4):(1~4); The second plant extract was prepared by the following method: ginger root powder was mixed with an alcohol solution, the mixture was placed in a water bath for ultrasonic-assisted extraction, then heated for extraction, cooled, and filtered to obtain the second plant extract.

2. The plant compound extract according to claim 1, characterized in that, The clove leaves are the leaves of the clove tree (Oleaceae family), the scutellaria root is the root of the scutellaria plant (Lamiaceae family), and the balsam flower is the flower of the balsam plant (Balsatianaceae family).

3. A method for preparing a plant compound extract, characterized in that, include: Preparation of the first plant extract: Take clove leaf powder, scutellaria root powder and balsam powder, mix with water, add cellulase, pectinase and lipase for enzymatic hydrolysis to obtain an enzymatic hydrolysate; add water to the enzymatic hydrolysate, heat to extract, filter, enrich the active substances in the filtrate with macroporous resin D101, and then add alcohol solution for desorption to obtain the first plant extract; wherein, the mass ratio of clove leaf powder, scutellaria root powder and balsam powder is (2~5):(1~4):(1~4); Preparation of the second plant extract: Take ginger root powder, add alcohol solution and mix, place the mixture in a water bath for ultrasonic-assisted extraction, then heat extraction, cool down, filter, and obtain the second plant extract; Preparation of plant compound extract: The first plant extract and the second plant extract are mixed at a mass ratio of (15~25):1, filtered, and the plant compound extract is obtained.

4. The method for preparing the plant compound extract according to claim 3, characterized in that, In the step of preparing the first plant extract The ratio of the mass of the cellulase to the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder is 0.01 to 0.

02. The ratio of the mass of the pectinase to the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder is 0.001 to 0.

002. The ratio of the mass of the lipase to the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder is 0.01 to 0.

02.

5. The method for preparing the plant compound extract according to claim 3, characterized in that, In the step of preparing the first plant extract, the mass of water added each time is 5 to 10 times the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder.

6. The method for preparing the plant compound extract according to claim 3, characterized in that, In the step of preparing the first plant extract, the mass of the macroporous resin D101 is 2 to 4 times the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder; the alcohol solution is selected from any one of the following aqueous solutions with a mass percentage of 70% to 90%: 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol, or butylene glycol; the mass of the alcohol solution is 10 to 12 times the sum of the masses of the clove leaf powder, the scutellaria root powder, and the balsam powder.

7. The method for preparing the plant compound extract according to claim 3, characterized in that, In the step of preparing the second plant extract, the alcohol solution is selected from any one of the following aqueous solutions with a mass percentage of 70% to 90%: 1,3-propanediol, glycerol, 1,2-propanediol, hexanediol, isopentylene glycol, or butanediol; the mass of the alcohol solution is 5 to 8 times the mass of the ginger root powder.

8. The use of the plant compound extract according to any one of claims 1 to 2 or the plant compound extract prepared by the preparation method according to any one of claims 3 to 7 in the preparation of personal care products with soothing and anti-inflammatory, oil-controlling or dandruff-removing effects.

9. A personal care product, characterized in that, The plant compound extract includes any one of claims 1 to 2 or the plant compound extract prepared by any one of claims 3 to 7.

10. The personal care product according to claim 9, characterized in that, The personal care product is selected from any one of toner, serum, cream, lotion, facial cleanser, and scalp care product; wherein the plant compound extract has a mass percentage content of 0.01% to 5% in the personal care product.

Images