A high-moisture replenishing repair composition, and a preparation method and application thereof

Abstract

The application discloses a high-moisture-retention, water-supplying and repairing composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics.The composition comprises an abroma zeylanicum extract, a dendrobium officinale extract, a opuntia tuna stem extract, a hydrolyzed oat and a common carrier for cosmetics.The opuntia tuna stem extract is used to actively supply water to provide sufficient water sources, the hydrolyzed oat is used to endogenously generate water to improve the water storage capacity of skin, the dendrobium officinale extract is used to physically lock water to prevent water loss, and the abroma zeylanicum extract is used to resist photoaging, improve skin color and whiten skin through antioxidation.The four components are combined to realize a complete skin care cycle from water supply to water locking, water generation and skin lightening.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a highly moisturizing and repairing composition, its preparation method, and its application. Background Technology

[0002] Skin hydration is fundamental to maintaining its health, elasticity, and barrier function. Traditional moisturizing techniques primarily rely on water-absorbing moisturizers (such as glycerin and hyaluronic acid) and occlusive moisturizers (such as mineral oil and petrolatum). However, these traditional strategies are often passive and fail to fully mimic the skin's natural dynamic moisturizing mechanism—a balanced system comprised of the active water-binding of the stratum corneum's natural moisturizing factor (NMF), water-locking by intercellular lipids, and the sealing of the sebum film. In recent years, utilizing plant-based active ingredients to simulate or enhance this natural mechanism has become a research hotspot.

[0003] Chinese patent CN107875089A discloses a skincare product containing Dendrobium officinale and its preparation method. This patent proposes a compounding of extracts from various plants, including Dendrobium officinale, oat kernels, Tremella fuciformis, and Opuntia ficus-indica. While this technical solution covers multiple plant sources with potential moisturizing effects, all components in this patent exist in the form of conventional solvent extracts or are simply physical mixtures. In particular, for the oat component, only conventional extraction processes are used to obtain oat polysaccharides or alkaloids, completely destroying the natural particulate skeleton structure of the filaggrin precursor in oat kernels. This treatment method causes the oat component to lose its function as a "slow-release reservoir of NMF precursors," only providing instantaneous surface moisturizing or anti-inflammatory effects, failing to achieve the continuous generation of endogenous NMFs. The compounding of its components is largely based on simple empirical combinations, lacking synergistic design at the microscopic level, resulting in moisturizing effects that often remain superficial, failing to address the fundamental problems of deep dehydration and barrier repair.

[0004] Chinese patent CN118557495A discloses a composition containing okra extract and dendrobium officinale extract. This technology primarily utilizes the flavonoids in okra extract to inhibit tyrosinase activity, thereby achieving a whitening effect, while the dendrobium officinale extract provides some moisturizing effect. However, the production of melanin in the skin is a complex biological process, directly regulated by tyrosinase and closely related to the inflammatory state of the skin microenvironment. This solution lacks potent anti-inflammatory and soothing components, making it difficult to block the induction of melanin production by inflammatory signaling pathways. Therefore, its effectiveness is often less than ideal when addressing whitening of sensitive skin or improving post-inflammatory hyperpigmentation.

[0005] Chinese patent CN102247306A discloses an oat extract, its preparation method, and its applications. This technology primarily uses enzymatic hydrolysis or solvent extraction to degrade large molecular proteins or polysaccharides in oats into smaller amino acids, peptides, or polysaccharide fragments, thereby improving their solubility and immediate absorption rate. While this technology reveals that enzymatic hydrolysis can improve the bioavailability of oat active ingredients, its teaching direction is "complete hydrolysis" and "complete extraction," aiming to obtain a clear extract. However, "complete hydrolysis" and "complete extraction" damage the intact particle skeleton structure and filaggrin precursors of oats, allowing for direct hydration but failing to achieve endogenous hydration and moisturizing.

[0006] Therefore, there is an urgent need to develop a new composition that can break through the limitations of existing simple compound formulations and construct a multi-dimensional moisturizing mechanism of "active hydration - endogenous water generation - physical water locking - anti-oxidation and whitening" through specific process control, so as to solve the technical problems in existing technologies such as difficulty in achieving both immediate and lasting moisturization, low efficiency of deep hydration, and single efficacy. Summary of the Invention

[0007] Therefore, the present invention provides a highly moisturizing and repairing composition, its preparation method and application, to solve the problems in the prior art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: According to a first aspect of the present invention, a highly moisturizing and repairing composition is provided, the composition comprising okra extract, dendrobium officinale extract, prickly pear stem extract, hydrolyzed oats, and a commonly used cosmetic carrier.

[0009] Furthermore, the composition comprises 1-5 parts of okra extract, 8-10 parts of dendrobium officinale extract, 2-8 parts of prickly pear stem extract, and 1-5 parts of hydrolyzed oats.

[0010] Opuntia ficus-indica stem extract: This active hydration layer activates the expression of the aquaporin AQP3 in keratinocytes, promoting active transmembrane water transport. It upgrades the process from "passive water absorption" to "active hydration," ensuring its moisturizing effect is unaffected by ambient humidity.

[0011] Hydrolyzed oats: This is an endogenous hydration layer. The granular skeletal structure gradually releases filaggrin precursors upon contact with water, which are then metabolized by the skin's own enzymes to generate natural moisturizing factors (NMF). This upgrades hydration from "exogenous hydration" to "endogenous hydration," fundamentally enhancing the skin's own moisturizing ability.

[0012] Dendrobium officinale extract: This acts as a physical moisture-locking layer. Dendrobium officinale polysaccharides form a uniform, breathable, and hydrophilic biomimetic membrane on the skin surface, instantly reducing transepidermal water loss. The biomimetic membrane structure is closer to the skin's natural barrier, allowing for breathability and preventing acne breakouts.

[0013] Okra extract: An antioxidant and whitening agent, rich in flavonoids, it scavenges free radicals, inhibits tyrosinase activity, and brightens skin tone. It provides antioxidant and whitening benefits while moisturizing, offering multiple synergistic effects.

[0014] Furthermore, the composition also includes trehalose and / or betaine.

[0015] Furthermore, the commonly used carrier in the cosmetic is selected from one or more of water, butylene glycol, glycerin, 1,2-pentanediol, and 1,2-hexanediol.

[0016] According to a second aspect of the present invention, a method for preparing a highly moisturizing and repairing composition includes: Step 1: Enzymatic hydrolysis and in-situ keyhole treatment Primary enzymatic hydrolysis: Mix finely powdered oats with buffer solution, add the first type of protease, and hydrolyze for 5-10 minutes at pH 6.0-7.0 and 30-35℃ until the degree of surface hydrolysis reaches 1%-2%; Secondary enzymatic hydrolysis: Adjust the pH to 7.0-8.0 and the temperature to 35-40℃, add the second protease or adjust the enzyme concentration, and continue enzymatic hydrolysis for 5-15 minutes until the overall degree of hydrolysis reaches 2%-5%; In-situ film formation: Before heating and inactivation, add 0.1%-0.5% of natural film-forming agent by weight of oat flour to allow it to self-assemble on the particle surface to form a nanoscale semi-permeable protective film. Inactivation: Heat the enzymatic hydrolysis system to 80-90℃ and maintain it for 5-15 minutes to inactivate the protease and obtain the inactivated enzymatic hydrolysate. Filter the enzymatic hydrolysate to obtain hydrolyzed oat wet flour filter cake. Step 2, Active Preloading The extract of Opuntia ficus-indica stem was uniformly loaded onto the wet filter cake of hydrolyzed oat flour in the form of spray, and then dried to a moisture content of ≤8% to obtain loaded gradient enzymatic hydrolyzed oat composite flour. Step 3, Mix Commonly used cosmetic carriers are mixed and heated to 30-40℃ and stirred to dissolve, resulting in a mixed matrix. The mixed matrix is ​​then cooled to 2-10℃, and under inert gas protection, extracts of Abelmoschus manihot, Dendrobium officinale, and supported gradient enzymatic hydrolyzed oat compound powder are added. The mixture is stirred at low speed until completely dispersed. The resulting liquid is homogenized 1-2 times under a pressure of 10-20 MPa, filtered, and discharged to obtain the final product.

[0017] Furthermore, both the first and second proteases are selected from bromelain and / or papain.

[0018] Furthermore, the natural film-forming agent is chitosan, with a mass ratio of 0.2-0.4:100 to oat flour.

[0019] Furthermore, in step three, the stirring speed is 20-50 rpm, the stirring time is 15-30 minutes, and the temperature is kept ≤10℃ throughout the process.

[0020] The degree of hydrolysis is strictly controlled at 2%-5%, which is between "unhydrolyzed" and "completely hydrolyzed". This preserves the particle skeleton structure while exposing enough enzyme cleavage sites to achieve the "pre-activated sustained release" function. The first-stage surface hydrolysis degree is 1%-2%, and the second-stage overall hydrolysis degree is 2%-5%. A "core-shell" hydrolysis degree gradient structure is constructed to achieve a biomimetic release curve that is fast at first, then slow, and then stable, perfectly simulating the skin's natural NMF generation process.

[0021] Chitosan self-assembles on the particle surface to form a nanoscale semi-permeable protective film (film thickness 10-100nm); it delays the rapid loss of macromolecules, achieves dual slow-release control of "skeleton + surface film", and extends the duration of effect to more than 8 hours.

[0022] Prickly pear cactus extract is spray-loaded onto the surface of wet oat powder, achieving microscopic spatial synergy between the "water source" (NMF precursor) and the "water pump" (AQP3 activator), allowing them to act simultaneously within the same microenvironment, with a synergistic efficiency increased by 3-5 times. Spray loading enriches the cactus extract on the surface of oat particles, creating a locally high-concentration microenvironment. When the particles swell upon contact with water, the cactus extract and filaggrin precursor are released synchronously, achieving a "spatiotemporally consistent" synergistic effect. This spatial proximity effect increases the local concentration of signaling molecules by 2-3 times, thereby improving AQP3 activation efficiency by 20%-30%, consistent with the typical range of "spatial co-localization of signaling molecules" in biology. Simultaneously, loading onto the particle surface reduces oxidative degradation of the extract during storage, further ensuring its biological activity.

[0023] The combination of okra flavonoids for antioxidant effects and Dendrobium officinale polysaccharides for film formation locks in moisture while combating free radicals and preventing oxidative damage to the skin barrier. Cactus activates AQP3, and oats generate NMF (Natural Moisturizing Factor), creating a synergistic effect between the "water source" and "water pump" for significantly enhanced hydration. Okra inhibits tyrosinase, and cactus activates water channels, allowing whitening ingredients to penetrate more easily in a well-hydrated environment, thus improving whitening effects. Dendrobium officinale polysaccharides form a film, while oat particles provide slow-release hydration, locking in moisture on the outer layer and generating moisture on the inner layer, creating a complete moisturizing cycle.

[0024] The application of a highly moisturizing and repairing composition provided by a third aspect of the present invention in the preparation of skin care products with moisturizing, hydrating, and skin barrier repairing effects.

[0025] Furthermore, the skincare product is a moisturizing toner, moisturizing serum, moisturizing lotion, moisturizing cream, moisturizing mask, or moisturizing spray.

[0026] The present invention has the following advantages: This invention utilizes prickly pear stem extract to actively replenish moisture and provide ample water, hydrolyzed oats to enhance the skin's own water retention capacity, Dendrobium officinale extract to physically lock in moisture and prevent water loss, and Abelmoschus manihot extract to anti-oxidize, whiten, improve skin tone, and resist photoaging. The four ingredients work together to achieve a complete skin care cycle from "replenishing moisture" to "locking in moisture" to "generating moisture" and then to "brightening".

[0027] The composition of this invention has a faster response speed; it does not depend on the user's own skin enzyme activity, and dry / aging skin (with low enzyme activity) can also achieve the same effect, making it more universal; the dual sustained-release mechanism ensures continuous moisturizing for more than 8 hours, and the sustained release is longer-lasting; at the same time, it achieves the triple effects of hydration, water generation, and water locking, making the effects more comprehensive.

[0028] This invention loads cactus extract in a wet powder state, utilizing the wet state of the particle surface to improve the uniformity of loading, and forms a stable adhesion after drying; adding okra and dendrobium officinale at 2-10℃ maximizes the preservation of the bioactivity of heat-sensitive active ingredients (flavonoids and polysaccharide structures are not destroyed). Attached Figure Description

[0029] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0030] The structures, proportions, sizes, etc. illustrated in this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed herein, and are not intended to limit the conditions under which the present invention can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size, without affecting the effects and objectives that the present invention can produce, should still fall within the scope of the technical content disclosed in the present invention.

[0031] Figure 1 A process flow diagram of a highly moisturizing and repairing composition provided in Embodiment 1 of the present invention; Figure 2 This is a four-dimensional three-dimensional moisturizing principle diagram of the high moisturizing and repairing composition provided in Example 1 of the present invention; Figure 3 This is a graph showing the effect of each sample on AQP3 expression provided in Experimental Example 2 of the present invention; Figure 4 This is a comparison chart of the moisturizing efficacy test results of various samples provided in Experimental Example 5 of the present invention. Detailed Implementation

[0032] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] Unless otherwise specified in the embodiments of this invention, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available products; different manufacturers and models of raw materials do not affect the implementation of the technical solution or the achievement of the technical effect of this invention.

[0034] Stem extract of Opuntia ficus-indica: Guangzhou Luojie Biotechnology Co., Ltd.

[0035] Bromelain: Source leaf, S10000.

[0036] Papain: Source leaf, S10011.

[0037] Arbutin extract: Xi'an Tianguangyuan Biotechnology Co., Ltd.; Dendrobium officinale extract: Shaanxi Yunhe Biotechnology Co., Ltd.

[0038] Example 1 A highly moisturizing and repairing composition, with the following formula by weight: 3 parts of okra extract, 9 parts of dendrobium officinale extract, 5 parts of prickly pear stem extract, 3 parts of hydrolyzed oats, 2 parts of trehalose, 1 part of betaine, 5 parts of glycerin, 4 parts of butylene glycol, 2 parts of 1,2-pentanediol, and water to make up to 100 parts.

[0039] Process flow diagram as follows Figure 1 As shown, the preparation method is as follows: Step 1: Enzymatic hydrolysis and in-situ keyhole treatment Primary enzymatic hydrolysis: Finely ground oat kernels (50 μm particle size) were mixed with pH 6.5 phosphate buffer at a mass ratio of 1:5. 0.3% bromelain by weight of the finely ground oat kernels was added, and the mixture was enzymatically hydrolyzed at 32°C for 8 minutes until the degree of surface hydrolysis reached 1.5%. Secondary enzymatic hydrolysis: Adjust the pH to 7.5 and the temperature to 38℃, add 0.2% papain by weight of fine oat kernel powder, and continue enzymatic hydrolysis for 10 minutes until the overall degree of hydrolysis reaches 3.5%; In-situ film formation: Before heating and inactivation, add 0.3% by weight of chitosan (pre-dissolved in 1% acetic acid solution) of oat flour and stir to allow the chitosan to self-assemble on the particle surface to form a nanoscale semi-permeable protective film. Inactivation: The enzymatic hydrolysis system is heated to 85°C and maintained for 10 minutes to inactivate the protease, resulting in inactivated hydrolysate. The hydrolysate is then filtered to obtain hydrolyzed oat wet powder filter cake (moisture content approximately 50%). Step 2, Active Preloading The extract of Opuntia ficus-indica stem was uniformly loaded onto the surface of hydrolyzed oat wet powder filter cake by spraying. During the spraying process, the wet powder was kept in a state of tumbling. Then, it was freeze-dried until the moisture content was ≤8% to obtain loaded gradient enzymatic hydrolyzed oat composite powder. Step 3, Mix Water, glycerin, butylene glycol, and 1,2-pentanediol were mixed, and trehalose and betaine were added. The mixture was heated to 35°C and stirred until dissolved to obtain a mixed matrix. The mixed matrix was cooled to 6°C, and under nitrogen protection, okra extract, dendrobium officinale extract, and the loaded gradient enzymatically hydrolyzed oat complex powder prepared in step two were added. The mixture was stirred at 30 rpm for 20 minutes until completely dispersed. The resulting liquid was homogenized twice under a pressure of 15 MPa and filtered through a 200-mesh filter to obtain the product. The four-dimensional moisturizing principle diagram is shown below. Figure 2 As shown.

[0040] Example 2 The weight proportions of each component in the formula were adjusted as follows: 1 part okra extract, 8 parts Dendrobium officinale extract, 2 parts Opuntia ficus-indica stem extract, and 1 part hydrolyzed oats. The preparation method is the same as in Example 1.

[0041] Example 3 The difference from Example 1 is that the weight parts of each component in the formula are adjusted as follows: 5 parts of okra extract, 10 parts of Dendrobium officinale extract, 8 parts of Opuntia ficus-indica stem extract, and 5 parts of hydrolyzed oats. The preparation method is the same as in Example 1.

[0042] Example 4 The difference from Example 1 is that bromelain was used for both the first and second proteases in the enzymatic hydrolysis process, and the two-stage enzymatic hydrolysis was achieved by adjusting the enzyme concentration. Specifically, 0.4% bromelain was added for the first-stage enzymatic hydrolysis, and 0.2% bromelain was added for the second-stage enzymatic hydrolysis. The rest was the same as in Example 1.

[0043] Example 5 The difference from Example 1 is that trehalose and betaine are not added. Otherwise, it is the same as Example 1.

[0044] Example 6 The difference from Example 1 is that the hydrolyzed oats are oat kernel powder and hydrolyzed oat protein (Xi'an Tianguangyuan Biotechnology Co., Ltd.) mixed in a 9:1 ratio. Step 1 is replaced by: mixing oat kernel powder and hydrolyzed oat protein with water and keeping the moisture content at 50% to obtain hydrolyzed oat wet powder filter cake; the rest is the same as in Example 1.

[0045] Example 7 The composition prepared in Example 1 was thickened by adding 0.1 parts of carbomer and 0.2 parts of sodium hyaluronate to adjust the viscosity, thus preparing a moisturizing essence. During preparation, the carbomer was first dispersed and swollen in water before the mixing in step three.

[0046] Examples 8-12 Different process parameters are shown in Table 1: Table 1 Different process parameters

[0047] Everything else is the same as in Example 1.

[0048] Comparative Example 1 The difference from Example 1 is that the okra extract was replaced with an equal amount of arbutin extract.

[0049] Comparative Example 2 The difference from Example 1 is that the Dendrobium officinale extract was replaced with an equal amount of hyaluronic acid.

[0050] Comparative Example 3 The difference from Example 1 is that the prickly pear cactus stem extract was replaced with an equal amount of tremella extract.

[0051] Comparative Example 4 The difference from Example 1 is that hydrolyzed oats were replaced with an equal amount of oat kernel extract.

[0052] Comparative Example 5 The difference from Example 1 is that hydrolyzed oats were replaced with an equal amount of finely ground oat kernels (without enzymatic hydrolysis).

[0053] Comparative Example 6 The difference from Example 1 is that the preparation method does not involve in-situ film formation (chitosan is not added in step one).

[0054] Comparative Example 7 The difference from Example 1 is that the preparation method does not involve preloading of the active ingredient (step two involves simply mixing the extract of Opuntia ficus-indica stem with hydrolyzed oats and then drying).

[0055] Comparative Example 8 The difference from Example 1 is that the mixing temperature in step three of the preparation method is 40°C (without low-temperature protection).

[0056] Comparative Example 9 The difference from Example 1 is that all raw materials are added at once using a traditional process, heated to 80°C and stirred for 30 minutes, and then directly cooled and discharged.

[0057] Comparative Example 10 The difference from Example 1 is that there are 6 parts of Abelmoschus moschatus extract, 11 parts of Dendrobium officinale extract, 9 parts of Opuntia streptacantha stem extract, and 6 parts of hydrolyzed oats. The rest is the same as in Example 1. Exceeding the dosage range defined in the present invention may bring a slight improvement in efficacy, but it will significantly increase the risk of skin irritation and disrupt the balance between safety and efficacy.

[0058] Experimental Example 1 Irritation experiments on Examples 1-12 and Comparative Examples 1-10: 1. The irritation of the product was evaluated by the chick embryo chorioallantoic membrane vascular assay (CAMVA). A certain amount of the test substance (40 μL) was directly contacted with the chorioallantoic membrane of the chick embryo. After acting for a period of time (30 min), the degree of vascular damage of the chorioallantoic membrane (such as ghost vessels, capillary congestion or bleeding) was observed, and different degrees of vascular damage were scored to judge the irritation of the test substance. The irritation of the test substance was compared according to the average score of the vascular damage degree (i.e., the NC value).

[0059] Evaluation criteria: (1) 0 < NC ≤ 2: It shows stress irritation, that is, non-damaging irritation. At this time, there is no bleeding damage to the capillaries.

[0060] (2) 2 < NC ≤ 3: It shows slight irritation, that is, slight damaging irritation has occurred at this time, and slight bleeding damage to the capillaries appears.

[0061] (3) 3 < NC ≤ 5: It shows moderate irritation, that is, relatively serious damaging irritation has occurred at this time, and relatively serious bleeding damage to the capillaries appears, which may be irreversible damage.

[0062] (4) NC > 5: It shows severe irritation, that is, extremely serious damaging irritation has occurred at this time, with large-area bleeding of the capillaries and a large amount of bleeding, which is irreversible damage.

[0063] (5) 10 chick embryos were taken for each sample for testing, and the results are shown in Table 2.

[0064] Table 2 Irritation test results

[0065] As can be seen from Table 2, the NC values of Examples 1-12 and Comparative Examples 1-9 of the present invention are all between 0 and 2, showing stress irritation and non-damaging irritation. It shows that the compositions of the present invention and each comparative example composition have good safety and no irritation to the skin. However, slight irritation occurred in Comparative Example 10, indicating that exceeding the range amount will cause slight irritation. In subsequent tests, Comparative Example 10 will no longer be tested.

[0066] 2. Subsequent Examples 1-12 and Comparative Examples 1-9 of the present invention showed no skin allergic reactions in guinea pig skin sensitization tests and multiple skin irritation tests in New Zealand rabbits; no skin phototoxicity was observed in guinea pig skin phototoxicity tests; and no toxicity was found in mice via oral toxicity grading.

[0067] Experiment Example 2 Hydration and moisturizing ability test: 1. AQP3 expression test of Examples 1-12 and Comparative Examples 1-9: Human skin keratinocytes (HaCaT) were used at a concentration of 5 × 10⁻⁶. 4 Cells were seeded in 24-well plates and cultured for 24 h. Then, 1% concentrations of the test samples (diluted with DMEM medium) were added, and the plates were cultured for another 24 h. AQP3 protein expression levels were detected by Western blotting, using β-actin as an internal control, and the relative expression levels were calculated (the blank control group was set at 100%).

[0068] 2. NMF generation tests of Examples 1-12 and Comparative Examples 1-9: Healthy subjects (10 per group) were selected. A test area was marked on the inner forearm of each subject, and the sample was applied twice daily for 7 consecutive days. Before and after use, keratinocytes were collected using an adhesive tape peeling method, and the total free amino acid content was measured using an amino acid analyzer to calculate the increase rate.

[0069] The results are shown in Table 3.

[0070] Table 3. Effects of each sample on AQP3 expression and NMF generation

[0071] From Table 3 and Figure 3It can be seen that the AQP3 expression level in Example 1 reached 275%, which was 175% higher than that in the blank control group (100%), proving that the prickly pear stem extract effectively activated the aquaporin AQP3, achieving active hydration. The AQP3 expression level in Comparative Example 3 was only 128%, significantly lower than that in Example 1, proving that the prickly pear stem extract is the key component for activating AQP3. The AQP3 expression level in Comparative Example 7 was 220%, lower than that in the active pre-loading group of Example 1, with a difference of about 55 percentage points, representing an increase in efficacy of about 25%. This difference scientifically and reasonably proves the core value of the "active pre-loading" technology: by directly anchoring the cactus extract to the surface of oat particles, the microscopic spatial synergy of the "water source" (NMF precursor) and the "water pump" (AQP3 activator) is achieved. The stratum corneum amino acid content of Example 1 increased by 42.5%, proving that hydrolyzed oats successfully metabolized into NMF in the skin, achieving endogenous hydration. The amino acid increase rate in Comparative Example 4 was only 12.5%, significantly lower than that in Example 1, demonstrating that hydrolyzed oats are a key component of endogenous water. The amino acid increase rate in Comparative Example 7 was 28.5%, lower than the 42.5% in Example 1, a difference of approximately 49%. This difference stems from the microscopic spatial synergistic effect achieved by the "active pre-loading" process—anchoring cactus extract to the surface of oat particles, allowing the "water source" (NMF precursor) and the "pump activator" (AQP3 signaling molecule) to be released synchronously in time and space, forming a localized high-concentration microenvironment, thereby promoting the metabolic activity of keratinocytes and indirectly improving the synthesis efficiency of NMF. The synergistic effect (49%) is higher than that of AQP3 (25%) because AQP3 expression is directly affected by local concentration, while NMF synthesis is a cumulative effect of downstream metabolic processes, making it more sensitive to process optimization, but still controlled within a biologically reasonable range. The amino acid increase rate of Comparative Example 6 was 28.5%, the same as that of Comparative Example 7, proving that the in-situ film formation technology mainly contributes to the sustained release effect rather than directly promotes synthesis. The two processes have different optimization directions, but their final contribution to NMF accumulation is similar.

[0072] Experimental Example 3 Whitening experiments of the compositions in Examples 1-6 and Comparative Examples 1-9: 1. Tyrosinase inhibition experiment: The compositions of Examples 1-6 and Comparative Examples 1-9 were diluted to a mass concentration of 1% in DMEM culture medium solution, sterilized by passing through a 0.22 μm filter membrane, and then used for later use.

[0073] B16-F10 cells were seeded at a density of 8000 cells / well in 96-well plates and incubated at 37°C, 5% CO2, and saturated humidity for 48 h. After 48 h, the 96-well plates were removed, and the test solution was added, with four replicates for each concentration. A blank control and a positive control of 0.05% kojic acid were also included, with three replicates per well. Incubation continued for another 24 h after drug administration. After 24 h, the plates were removed, the old culture medium was discarded, and each well was washed once with 100 μL of PBS. Then, 40 μL of cell lysis buffer containing 1 mM PMSF was added to each well, and the plates were incubated at 4°C for 30 min for lysis. After lysis, the plate was placed in a 37°C incubator for 5 min to rewarm. Then, 100 μL of 1 mg / mL L-DOPA was added to each well, and the plate was incubated at 37°C for 2 h. After incubation, the absorbance at 492 nm was measured using a microplate reader, and the tyrosinase inhibition rate was calculated according to the following formula. The results are shown in Table 4.

[0074] Tyrosinase inhibition rate = [(OD 空白组 -OD 实验组 ) / OD 空白组 ×100%.

[0075] 2. Melanin Inhibition Experiment B16-F10 mouse melanoma cells were used at a rate of 2×10 5 Cells were seeded in 6-well plates and cultured for 24 hours. Then, 1% concentrations of the test samples were added, and the cells were cultured for another 48 hours. Cells were lysed, and absorbance at 492 nm was measured to calculate the melanin inhibition rate.

[0076] Melanin inhibition rate (%) = (OD 空白组 -OD 实验组 ) / OD 空白组 ×100% Table 4. Experimental results of inhibition rates of tyrosinase and melanin.

[0077] As shown in Table 4, Example 1 achieved a tyrosinase inhibition rate of 54.5% and a melanin inhibition rate of 47.3%. Comparative Example 1 showed a tyrosinase inhibition rate of only 35.6% and a melanin inhibition rate of only 21.5%, demonstrating that Okra extract is the core component for the whitening effect of this invention, and its effect is significantly superior to the traditional whitening agent arbutin. Comparative Example 4 showed a tyrosinase inhibition rate of 46.2% and a melanin inhibition rate of 32.3%, which, although higher than Comparative Example 1, was significantly lower than Example 1, demonstrating that hydrolyzed oats significantly promote the whitening effect by providing a hydrating environment and promoting the penetration of active ingredients. Comparative Example 3 showed a tyrosinase inhibition rate of 42.5% and a melanin inhibition rate of 28.2%, lower than Example 1, demonstrating that cactus promotes water penetration by activating AQP3, providing a better environment for the whitening ingredients to function. Comparative Example 2 showed a tyrosinase inhibition rate of 40.2% and a melanin inhibition rate of 26.3%, lower than Example 1, demonstrating that the film-forming and penetration-enhancing effects of Dendrobium officinale polysaccharides contribute uniquely to the improved whitening effect. Comparative Example 5 showed a lower whitening effect than Example 6, demonstrating that the active ingredients released by enzymatic hydrolysis contribute to the whitening efficacy. Comparative Examples 6 and 7 both showed lower whitening effects than Example 1, demonstrating the importance of in-situ keyhole and active pre-loading processes in maintaining the stability and synergistic effect of active ingredients. Comparative Examples 8 and 9 also showed lower whitening effects than Example 1, demonstrating the protective effect of low-temperature processes on heat-sensitive whitening ingredients. Example 6 showed a tyrosinase inhibition rate of 47.5% and a melanin inhibition rate of 38.6%, lower than Example 1, but still significantly better than Comparative Examples 1, 2, and 4, demonstrating that even with partial substitution processes, the present invention can still maintain good whitening efficacy.

[0078] Experiment Example 4 Anti-allergic and soothing tests of the compositions and serums in Examples 1-6 and Comparative Examples 1-9: 1. Fifteen recombinant human epidermal cells were selected and cultured for 1 hour using equal amounts of the compositions from Examples 1-6 and Comparative Examples 1-9, respectively. An inflammatory cytokine combination containing interleukin (IL)-4 (10 ng / mL), IL-13 (10 ng / mL), tumor necrosis factor (TNF)-α (5 ng / mL), and poly(I:C) (10 μg / mL) was used. After 24 hours, the release of PGE2 (prostaglandin E2, an inflammatory mediator, a common clinical assessment parameter for sensitive skin) in the cell culture medium was measured using ELISA. The results are shown in Table 5.

[0079] 2. Serum: The serum of Example 7 of this invention, Examples 2-6 and Comparative Examples 1-9 are prepared by adding equal amounts of sodium hyaluronate (0.1 parts) and carbomer (0.2 parts) as thickening and stabilizing agents according to Example 7, and adjusting the viscosity to prepare the serum. Before step one, carbomer is first dispersed and swollen in water to obtain the serums of Examples 2-6 and Comparative Examples 1-9 to ensure that the viscosity of all test samples is consistent.

[0080] Seventy-five volunteers with facial redness were selected and divided into 15 groups. After cleansing their faces, the subjects applied the essence compositions of different embodiments and comparative examples of the present invention evenly to their faces and gently massaged them until absorbed. They used the mixture twice a day, morning and evening, for 7 consecutive days. The reduction of facial redness was analyzed using the VISIA-CR facial image analyzer. The results are shown in Table 5.

[0081] Table 5. PGE2 release levels

[0082] As shown in Table 5, the PGE2 release in Example 1 was only 128.3 pg / mL, significantly lower than the blank control (245.6 pg / mL). The red zone a value and red zone area percentage in Example 7 both decreased, demonstrating the excellent anti-inflammatory and soothing effects of this invention. The PGE2 release in Comparative Examples 1, 2, 3, and 4 was significantly higher than in Example 1, and their facial redness improvement effects were significantly inferior to Example 7, demonstrating a significant synergistic effect of the four components in anti-inflammatory and soothing aspects. The PGE2 release in Comparative Example 1 was 185.3 pg / mL, with a red zone area decrease of only 6.78%, demonstrating the important role of flavonoids in anti-inflammatory and soothing effects. The PGE2 release in Comparative Example 3 was 198.5 pg / mL, the highest among all comparative examples, with a red zone area decrease of only 5.12%, demonstrating the important role of cactus in soothing sensitivity by regulating skin hydration. The anti-inflammatory effect of Comparative Example 5 was lower than that of Examples 1 and 7, demonstrating that the active ingredient released by enzymatic hydrolysis contributes to anti-inflammation. The anti-inflammatory effects of Comparative Examples 6 and 7 were lower than those of Examples 1 and 7, demonstrating the importance of in-situ keyhole and active pre-loading processes in maintaining the stability and sustained-release effect of the active ingredient. The anti-inflammatory effects of Comparative Examples 8 and 9 were also lower than those of Examples 1 and 7, demonstrating the protective effect of low-temperature processes on heat-sensitive anti-inflammatory components. The PGE2 release of Example 6 was 158.3 pg / mL, with a 3.85% decrease in the red zone a value and a 20.15% decrease in the red zone area percentage. Although lower than that of Example 1, it was still significantly better than Comparative Examples 1-5, demonstrating that even with a partially substituted process, the present invention can still maintain a good anti-inflammatory and soothing effect.

[0083] Experimental Example 5 Tests on the moisturizing efficacy of serums in Examples 2-7 and Comparative Examples 1-9 The test was conducted in accordance with QB / T 4256-2011 "Guidelines for Evaluation of Moisturizing Efficacy of Cosmetics".

[0084] Test samples: Examples 2-7, Comparative Examples 1-9. Examples 2-6 and Comparative Examples 1-9 were prepared by adding equal amounts of sodium hyaluronate (0.1 parts) and carbomer (0.2 parts) as thickening and stabilizing agents as described in Example 7, adjusting the viscosity to prepare the serum. Before step one, the carbomer was dispersed and swollen in water to obtain the serums of Examples 2-6 and Comparative Examples 1-9, to ensure that the viscosity of all test samples was consistent. Testing instruments: Corneometer CM825 skin moisture meter (CK, Germany), Tewameter TM Hex transdermal moisture loss meter (CK, Germany).

[0085] Test method: Healthy subjects (30 per group, aged 25-55 years) were selected. A 3cm × 3cm test area was marked on the inner forearm of each subject, and the baseline value (T0) was measured. The test was administered at 2 mg / cm². 2 The standard dosage was applied to the sample, and the skin stratum corneum moisture content and transepidermal water loss rate (TEWL) were measured at 15 minutes (T15min), 2 hours (T2), 4 hours (T4), and 8 hours (T8) after application. The rate of change relative to the baseline value was calculated at each time point. The test environment temperature was 21±1℃ and the relative humidity was 50±5%RH.

[0086] Evaluation indicators: Change rate of stratum corneum moisture content (%) = (T) n - T0) / T0×100%; T n T0 represents the moisture content of the stratum corneum at different times after use; T0 represents the moisture content of the stratum corneum before use.

[0087] TEWL change rate (%) = (W0 - W) n ) / W0×100%; W n - Transdermal water loss (TEWL) values ​​of the test area after different usage times; W0 - Initial value of water loss in the test area.

[0088] The results are shown in Table 6.

[0089] Table 6. Results of Moisturizing Efficacy Tests for Each Sample

[0090] From Table 6 and Figure 4It can be seen that Example 7 (Essence) achieved a moisture replenishment rate of 36.5% at T15min, the highest among all samples, demonstrating its excellent immediate hydration ability; at T8h, it still maintained a high level of 30.2%, with a decrease of only 17%, while TEWL decreased by 29.5%, proving that it formed a highly efficient biomimetic water-locking film through the synergistic effect of sodium hyaluronate and carbomer, significantly delaying moisture loss. Comparative Examples 1-4 had T15min values ​​of only 15.5%-21.5% and T8h values ​​of only 10.0%-15.0%, with a decrease of 30%-35%, far higher than the 17% of Example 7, demonstrating the indispensable synergistic effect of the four components—Abelmoschus mandshuriensis provides antioxidant support, Dendrobium officinale forms a water-locking film, Prickly pear activates active hydration, and hydrolyzed oats achieve endogenous water production; only through the synergy of these four components can "rapid hydration + long-lasting water retention" be achieved. The T8h / T15min ratio in Example 7 reached 0.83 (30.2 / 36.5), while that in Comparative Example 4 was only 0.65 (10.0 / 15.5). This significant difference demonstrates that hydrolyzed oats, through continuous metabolism, generate NMF, effectively replenishing the cuticle moisture over an 8-hour timescale and offsetting some of the evaporation loss. This is a scientific manifestation of the "endogenous water generation" mechanism—not "moisture increasing against the trend," but rather "significantly slowing down the rate of loss." Comparative Example 5 (unhydrolyzed oats) had a T8h of only 11.2%, a decrease of 33%, proving that "controlled enzymatic hydrolysis" is a prerequisite for activating endogenous water. Comparative Examples 6-7 (no in-situ film formation / no active preloading) had T8h values ​​of 17.0% and 17.8% respectively, with decreases greater than in Example 7, demonstrating the key role of dual sustained release and spatial synergy in prolonging the moisturizing effect. Comparative Examples 8-9 (no low-temperature protection / traditional process) had T8h values ​​of 18.5% and 13.0% respectively, further confirming that the low-temperature stepwise process is crucial for the protection of active ingredients. Example 6 (partially alternative process) had a T15min of 31.5% and a T8h of 25.0%, a decrease of 21%, which, although lower than in Example 7, was still significantly better than all comparative examples, proving the robustness and broad applicability of the technical solution of the present invention.

[0091] Experimental Example 6 Tests of high moisturizing efficacy in Examples 2-7 and Comparative Examples 1-9: Screening for dry skin (TEWL ≥ 25g / m 2Eighty volunteers, aged 30-40 years, were randomly divided into 16 groups of 5 participants each. Data on facial moisture was collected using the Transdermal Water Loss (TEWL) and Evaporative Heat Loss (EHW) probes of the Probe Tewameter™ Hex (CK, Germany), which served as baseline skin values. Participants used the serum twice daily, morning and evening. Data was collected 15 minutes after morning application, and repeated after 8 weeks of continuous use. Changes in skin moisture content before and after sample use were evaluated to determine the moisturizing efficacy. (TEWL is highly sensitive to temperature and humidity; measurements were taken at 21±1℃ and 50±10%RH, with participants required to remain seated and balanced for at least 15-30 minutes in the testing environment.)

[0092] Transepidermal water loss change rate TEWL% = (W n -W0) / W0, the final result is the average value, a negative value indicates reduced loss, that is, good moisturizing barrier repair effect.

[0093] In the formula, W n - Transdermal water loss (TEWL) values ​​of the test area after 15 minutes or 8 consecutive weeks; W0 - Initial value of water loss in the test area. Results are shown in Table 7.

[0094] Table 7. Results of transdermal water loss rate test for each sample on dry skin.

[0095] As shown in Table 7, Example 7 exhibited the strongest immediate barrier repair ability (W15min -25.0%) and long-term repair effect (W8 weeks -42.0%) on dry skin, representing increases of 220% and 140% respectively compared to Comparative Example 4 (dehydrated hydrolyzed oats). This fully demonstrates the synergistic advantages of the composition of the present invention and the superiority of the serum formulation. The order of the other examples is consistent with the moisturizing efficacy test, and the comparative data validates the necessity of the innovations in each component and process.

[0096] Experimental Example 7 Heat resistance, cold resistance, and thermal cycling tests were conducted in accordance with QB / T 2660-2004.

[0097] Test method: Heat resistance test: Maintain at 40±1℃ for 24 hours, then observe after returning to room temperature; Cold resistance test: Maintain at 5±1℃ for 24 hours, then observe after returning to room temperature; Hot and cold cycling: 40±1℃ 24h → room temperature 24h → 5±1℃ 24h, cycle 3 times; The test results are shown in Table 8.

[0098] Table 8 Stability test results for each sample

[0099] As shown in Table 8, Examples 1-12 and Comparative Examples 1-8 all passed various stability tests. After heat resistance, cold resistance, and thermal cycling, there was no stratification, no floating oil, and the color remained stable, proving that the compositions of the present invention have excellent physicochemical stability under normal storage conditions. Comparative Example 8 showed slight turbidity after thermal cycling, while Examples 1-12 remained stable, demonstrating the important role of the low-temperature process in maintaining system stability. High temperatures may cause some active ingredients to aggregate or precipitate. Comparative Example 9 showed slight stratification and a small amount of floating oil after thermal cycling, while Examples 1-12 remained stable, proving the key role of the stepwise feeding process of "high-temperature swelling - medium-temperature dispersion - low-temperature activation" in the stability of the system, which can avoid the interaction and instability caused by the simultaneous addition of multiple components at high temperatures. Comparative Examples 6 and 7 both passed stability tests, but their efficacy data showed lower values ​​than Example 1, proving that in-situ film formation and pre-loading of active ingredients mainly contribute to sustained release and synergistic effects, rather than physical stability. The stability of Example 7 is comparable to that of Example 1, demonstrating that the physical stability of the composition of the present invention is not affected even with partial substitution processes, reflecting the robustness of the formulation.

[0100] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A highly moisturizing and repairing composition, characterized in that, The composition includes okra extract, dendrobium officinale extract, prickly pear stem extract, hydrolyzed oats, and a commonly used cosmetic carrier.

2. The highly moisturizing and repairing composition according to claim 1, characterized in that, The composition comprises 1-5 parts of okra extract, 8-10 parts of dendrobium officinale extract, 2-8 parts of prickly pear stem extract, and 1-5 parts of hydrolyzed oats.

3. The highly moisturizing and repairing composition according to claim 1, characterized in that, The composition also includes trehalose and / or betaine.

4. The highly moisturizing and repairing composition according to claim 1, characterized in that, The commonly used carriers in cosmetics are selected from one or more of water, butylene glycol, glycerin, 1,2-pentanediol, and 1,2-hexanediol.

5. A method for preparing a highly moisturizing and repairing composition, characterized in that, The method includes: Step 1: Enzymatic hydrolysis and in-situ keyhole treatment Primary enzymatic hydrolysis: Mix finely powdered oats with buffer solution, add the first type of protease, and hydrolyze for 5-10 minutes at pH 6.0-7.0 and 30-35℃ until the degree of surface hydrolysis reaches 1%-2%; Secondary enzymatic hydrolysis: Adjust the pH to 7.0-8.0 and the temperature to 35-40℃, add the second protease or adjust the enzyme concentration, and continue enzymatic hydrolysis for 5-15 minutes until the overall degree of hydrolysis reaches 2%-5%; In-situ film formation: Before heating and inactivation, add 0.1%-0.5% of natural film-forming agent by weight of oat flour to allow it to self-assemble on the particle surface to form a nanoscale semi-permeable protective film. Inactivation: Heat the enzymatic hydrolysis system to 80-90℃ and maintain it for 5-15 minutes to inactivate the protease and obtain the inactivated enzymatic hydrolysate. Filter the enzymatic hydrolysate to obtain hydrolyzed oat wet flour filter cake. Step 2, Active Preloading The extract of Opuntia ficus-indica stem was uniformly loaded onto the wet filter cake of hydrolyzed oat flour in the form of spray, and then dried to a moisture content of ≤8% to obtain loaded gradient enzymatic hydrolyzed oat composite flour. Step 3, Mix Commonly used cosmetic carriers are mixed and heated to 30-40℃ and stirred to dissolve, resulting in a mixed matrix. The mixed matrix is ​​then cooled to 2-10℃, and under inert gas protection, extracts of Abelmoschus manihot, Dendrobium officinale, and supported gradient enzymatic hydrolyzed oat compound powder are added. The mixture is stirred at low speed until completely dispersed. The resulting liquid is homogenized 1-2 times under a pressure of 10-20 MPa, filtered, and discharged to obtain the final product.

6. The preparation method according to claim 5, characterized in that, In step two, both the first protease and the second protease are selected from bromelain and / or papain.

7. The preparation method according to claim 5, characterized in that, In step one, the natural film-forming agent is chitosan, and its mass ratio with oat flour is 0.2-0.4:

100.

8. The preparation method according to claim 5, characterized in that, In step three, the stirring speed is 20-50 rpm, the stirring time is 15-30 minutes, and the temperature is kept ≤10℃ throughout the process.

9. The application of a highly moisturizing and repairing composition in the preparation of skin care products with moisturizing, hydrating, and skin barrier repairing effects.

10. The application according to claim 9, characterized in that, The skincare products mentioned are moisturizing water, moisturizing serum, moisturizing lotion, moisturizing cream, moisturizing mask, or moisturizing spray.

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