A gynecological gel for inhibiting bacteria, regulating microecological balance and tightening in vagina and a preparation method thereof
By combining raw materials such as carbomer, sodium hydroxide, and collagen, and by making our own collagen, we have solved the problem of poor antibacterial and microecological balance effects of existing gynecological gels, and achieved antibacterial, microecological balance regulation, and tightening effects in the vagina.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HUNAN MICRO PEPTIDE BIOMEDICAL CO LTD
- Filing Date
- 2026-04-20
- Publication Date
- 2026-06-05
AI Technical Summary
Existing gynecological gels suffer from limitations in their preparation methods and raw material selection, affecting their antibacterial and microecological balance effects. They also lack tightening effects, making them difficult to effectively treat highly recurrent vaginal inflammation.
Using raw materials such as carbomer, sodium hydroxide, collagen, sodium hyaluronate, and isomaltooligosaccharide, combined with collagen prepared from homemade buckwheat filtrate, the product enhances antibacterial and microecological balance effects through the synergistic effect of multiple components, and promotes collagen fiber synthesis and repair.
It achieves antibacterial, microecological balance regulation and tightening effects in the vagina, significantly reduces the risk of inflammation and infection, promotes tissue elasticity, and reduces the adhesion and invasion of pathogenic bacteria.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of daily chemical preparation technology, specifically relating to a gynecological gel for vaginal antibacterial, microecological balance regulation and tightening and its preparation method. Background Technology
[0002] Vaginal inflammation is the most common gynecological disease in women, with an incidence rate as high as 70%-80%. Its causes are diverse, mainly including bacterial vaginosis, vulvovaginal candidiasis (yeast infection), trichomonal vaginitis, as well as senile vaginitis, mixed vaginitis, etc. Vaginal inflammation has a high recurrence rate and requires long-term treatment. If it is not treated in time, it may lead to cervicitis, pelvic inflammatory disease, and even affect fertility.
[0003] Gynecological gels are commonly used to eliminate various vaginal inflammations. For example, Chinese patent CN 114903801 A describes a silk protein gynecological gel and its preparation method. The gynecological gel prepared by mixing a gelling agent, solubilizer, neutralizing agent, and deionized water exhibits good stability and antibacterial properties. After use, it can reduce the erosion area, promote the healing of cervical erosion, alleviate local inflammation, reduce increased vaginal discharge, and improve symptoms such as pain, bloody discharge, and purulent discharge. It is suitable for symptoms such as cervicitis, vaginitis, and cervical erosion. However, the preparation method and raw materials of gynecological gels can affect their effectiveness. If a simple, antibacterial, microecologically balanced, and firming gynecological gel could be provided, it would be beneficial in offering a scientific treatment method for a wider range of patients. Summary of the Invention
[0004] The purpose of this invention is to provide a gynecological gel for vaginal antibacterial, microecological balance regulation and tightening, and its preparation method. The preparation method is simple and has the effects of antibacterial, microecological balance regulation and tightening.
[0005] This invention provides a gynecological gel for vaginal antibacterial, microecological balance regulation, and vaginal tightening, comprising the following raw materials by weight: Carbomer 0.5-1 parts, sodium hydroxide 0.04-0.09 parts, collagen 1-3 parts, sodium hyaluronate 0.01-0.06 parts, isomaltooligosaccharide 0.5-1.5 parts, propylene glycol 2-5 parts, methylparaben 0.05-0.3 parts, ethylparaben 0.02-0.15 parts, hydrogenated lecithin 0.1-1.5 parts, 1,3-butanediol 1-6 parts, glycerin 3-5 parts, xanthan gum 0.5-1 parts, and water 70-90 parts.
[0006] Preferably, the ingredients, by weight, include the following raw materials: Carbomer 0.5 parts, sodium hydroxide 0.09 parts, collagen 2 parts, sodium hyaluronate 0.02 parts, isomaltooligosaccharide 0.5 parts, propylene glycol 2 parts, methylparaben 0.05 parts, ethylparaben 0.02 parts, hydrogenated lecithin 0.1 parts, 1,3-butanediol 1 part, glycerin 5 parts, xanthan gum 1 part, and water 87.72 parts.
[0007] Preferably, the method for preparing the collagen includes: S1. Soak the cod skin in ethanol solution and citric acid solution in turn to obtain soaked fish skin; S2. Fish skin, compound enzyme and buckwheat filtrate are mixed and enzymatically hydrolyzed, and after enzyme inactivation and solid-liquid separation, a crude collagen extract is obtained; the compound enzyme includes: papain and phospholipase C; S3. Mix the crude collagen liquid and ammonium sulfate, stir, let stand, and centrifuge to obtain a precipitate; S4. After mixing the precipitate with phosphate buffer, perform chromatography and centrifugation to obtain the supernatant; concentrate and freeze-dry the supernatant to obtain collagen.
[0008] Preferably, in step S1, the volume concentration of the ethanol solution is 70%-80%; The citric acid solution has a mass concentration of 0.03%-0.08%.
[0009] Preferably, in step S2, papain and phospholipase C are mixed at a mass ratio of 0.5-0.8:1 to obtain a composite enzyme.
[0010] Preferably, in step S2, the mass ratio of fish skin, compound enzyme and buckwheat filtrate is 10-15:5-8:75-90; The temperature for the mixed enzymatic hydrolysis is 50-60℃, and the time is 8-10h.
[0011] Preferably, the buckwheat filtrate is prepared by mixing buckwheat and water at a mass ratio of 1:4-8 at 40-45℃ and soaking for 10-12 hours, followed by filtration to obtain the buckwheat filtrate.
[0012] Preferably, in step S3, during the settling process, the mass ratio of crude collagen solution to ammonium sulfate is 100:1-1.5. The standing time is 2-4 hours, and the temperature is 25-30℃.
[0013] Preferably, in step S4, the mass-to-volume ratio of the precipitate to the phosphate buffer is 0.1-0.5 g: 20-30 mL; The phosphate buffer solution has a pH of 7.0-7.5 and a concentration of 150-200 mM.
[0014] This invention provides a method for preparing the gynecological gel described in the above technical solution, comprising the following steps: Carbomer, sodium hydroxide, collagen, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, methylparaben, ethylparaben, hydrogenated lecithin, 1,3-butanediol, glycerin, xanthan gum, and water are mixed evenly to obtain a gynecological gel.
[0015] Beneficial effects: This invention provides a gynecological gel for vaginal antibacterial, microecological balance regulation, and vaginal tightening, comprising the following raw materials by weight: 0.5-1 parts carbomer, 0.04-0.09 parts sodium hydroxide, 1-3 parts collagen, 0.01-0.06 parts sodium hyaluronate, 0.5-1.5 parts isomaltooligosaccharide, 2-5 parts propylene glycol, 0.05-0.3 parts methylparaben, 0.02-0.15 parts ethylparaben, 0.1-1.5 parts hydrogenated lecithin, 1-6 parts 1,3-butanediol, 3-5 parts glycerin, 0.5-1 parts xanthan gum, and 70-90 parts water.
[0016] This invention utilizes buckwheat filtrate to prepare collagen. The active ingredients in the buckwheat filtrate exhibit antibacterial activity, penetrating the cell membranes of pathogenic bacteria and disrupting their lipid bilayer. The collagen can repair the vaginal mucosal barrier, reducing the adhesion and invasion of pathogenic bacteria, thus indirectly lowering the risk of inflammatory infection. Combining the prepared collagen with carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, and other raw materials promotes synergistic effects of multiple components, thereby enhancing collagen fiber synthesis and repair, and improving tissue elasticity. Therefore, the gynecological gel prepared by this invention can be used for vaginal antibacterial activity, regulation of microecological balance, and vaginal tightening. Detailed Implementation
[0017] Unless otherwise specified, all raw materials, equipment and methods used in this invention are conventionally purchased.
[0018] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0019] Example 1 The steps for preparing a gynecological gel for vaginal antibacterial, microecological balance regulation, and vaginal tightening are as follows: (1) Preparation of collagen Cod (Pacific cod) Gadus macrocephalus Clean the fish skin, drain the water, and cut it into small pieces of 1cm×1cm to obtain the processed fish skin; At 28-30℃, the treated fish skin was completely immersed in a 70% ethanol solution for 4 hours. After that, the fish skin was rinsed with water. Then, the rinsed fish skin was completely immersed in a 0.08% citric acid solution for 4 hours. After rinsing with water, the soaked fish skin was obtained. At 40℃, buckwheat (specifically bitter buckwheat) is placed in a container. Fagopyrum tataricum (L.) Gaertn.) was mixed with water at a mass ratio of 1:8 and soaked for 10 hours. After filtration, buckwheat filtrate was obtained. Soaked fish skin, compound enzyme (the compound enzyme is obtained by mixing papain and phospholipase C at a mass ratio of 0.5:1) and buckwheat filtrate at a mass ratio of 10:8:90 are mixed and homogenized, and then enzymatically hydrolyzed at 60℃ for 8 hours. After enzyme inactivation and solid-liquid separation, crude collagen extract is obtained. Collagen crude solution and ammonium sulfate (food grade) were mixed at a mass ratio of 100:1 and stirred until homogeneous. The mixture was then allowed to stand at 28-30℃ for 4 hours and centrifuged to obtain a precipitate. The precipitate was then mixed with phosphate buffer (pH 7.0, concentration 150mM) at a mass-to-volume ratio of 0.5g:20mL. The mixture was then chromatographed using G-75, 100, 150 dextran chromatography. The protein eluate was collected and centrifuged to obtain a supernatant. The supernatant was concentrated at 50℃ and -0.08MPa until the solid content of the solution reached 10%. The solution was then freeze-dried to obtain collagen.
[0020] (2) Preparation of gynecological gel By weight, the gynecological gel comprises the following ingredients: 0.5 parts carbomer, 0.09 parts sodium hydroxide, 2 parts collagen, 0.02 parts sodium hyaluronate, 0.5 parts isomaltooligosaccharide, 2 parts propylene glycol, 0.05 parts methylparaben, 0.02 parts ethylparaben, 0.1 parts hydrogenated lecithin, 1 part 1,3-butanediol, 5 parts glycerin, 1 part xanthan gum, and 87.72 parts water; Carbomer, sodium hydroxide, collagen, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, methylparaben, ethylparaben, hydrogenated lecithin, 1,3-butanediol, glycerin, xanthan gum, and water are mixed evenly to obtain a gynecological gel.
[0021] Example 2 The steps for preparing a gynecological gel for vaginal antibacterial, microecological balance regulation, and vaginal tightening are as follows: (1) Preparation of collagen Cod (Pacific cod) Gadus macrocephalus Clean the fish skin, drain the water, and cut it into small pieces of 1cm×1cm to obtain the processed fish skin; At 28-30℃, the treated fish skin was completely immersed in an 80% ethanol solution for 4 hours. After that, the fish skin was rinsed with water. Then, the rinsed fish skin was completely immersed in a 0.05% citric acid solution for 4 hours. After rinsing with water, the soaked fish skin was obtained. At 40℃, buckwheat (specifically bitter buckwheat, Fagopyrum tataricum (L.) Gaertn.) was mixed with water at a mass ratio of 1:8 and soaked for 10 hours. After filtration, buckwheat filtrate was obtained. Soaked fish skin, compound enzyme (the compound enzyme is obtained by mixing papain and phospholipase C at a mass ratio of 0.8:1) and buckwheat filtrate at a mass ratio of 10:8:90 are mixed and homogenized, and then enzymatically hydrolyzed at 60℃ for 8 hours. After enzyme inactivation and solid-liquid separation, crude collagen extract is obtained. Collagen crude solution and ammonium sulfate (food grade) were mixed at a mass ratio of 100:1 and stirred until homogeneous. The mixture was then allowed to stand at 28-30℃ for 4 hours and centrifuged to obtain a precipitate. The precipitate was then mixed with phosphate buffer (pH 7.0, concentration 150mM) at a mass-to-volume ratio of 0.5g:20mL. The mixture was then chromatographed using G-75, 100, 150 dextran chromatography. The protein eluate was collected and centrifuged to obtain a supernatant. The supernatant was concentrated at 50℃ and -0.08MPa until the solid content of the solution reached 10%. The solution was then freeze-dried to obtain collagen.
[0022] (2) Preparation of gynecological gel By weight, the gynecological gel comprises the following ingredients: 0.5 parts carbomer, 0.09 parts sodium hydroxide, 3 parts collagen, 0.02 parts sodium hyaluronate, 0.5 parts isomaltooligosaccharide, 2 parts propylene glycol, 0.05 parts methylparaben, 0.02 parts ethylparaben, 0.1 parts hydrogenated lecithin, 1 part 1,3-butanediol, 5 parts glycerin, 1 part xanthan gum, and 86.72 parts water; Carbomer, sodium hydroxide, collagen, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, methylparaben, ethylparaben, hydrogenated lecithin, 1,3-butanediol, glycerin, xanthan gum, and water are mixed evenly to obtain a gynecological gel.
[0023] Comparative Example 1 The only difference from Example 1 is that it uses Nile tilapia ( Oreochromis aureus ♂ × Oreochromis niloticus♀ Replace the cod in Example 1.
[0024] Comparative Example 2 The only difference from Example 1 is that step (1) in Comparative Example 2 is as follows: (1) Preparation of collagen Cod (Pacific cod) Gadus macrocephalus Clean the fish skin, drain the water, and cut it into small pieces of 1cm×1cm to obtain the processed fish skin; At 28-30℃, the treated fish skin was completely immersed in a 70% ethanol solution for 4 hours. After that, the fish skin was rinsed with water. Then, the rinsed fish skin was completely immersed in a 0.08% citric acid solution for 4 hours. After rinsing with water, the soaked fish skin was obtained. Soaked fish skin, compound enzyme (the compound enzyme is obtained by mixing papain and phospholipase C at a mass ratio of 0.5:1) and water at a mass ratio of 10:8:90 are mixed and homogenized, and then enzymatically hydrolyzed at 60℃ for 8 hours. After enzyme inactivation and solid-liquid separation, crude collagen extract is obtained. Collagen crude solution and ammonium sulfate (food grade) were mixed at a mass ratio of 100:1 and stirred until homogeneous. The mixture was then allowed to stand at 28-30℃ for 4 hours and centrifuged to obtain a precipitate. The precipitate was then mixed with phosphate buffer (pH 7.0, concentration 150mM) at a mass-to-volume ratio of 0.5g:20mL. The mixture was then chromatographed using G-75, 100, 150 dextran chromatography. The protein eluate was collected and centrifuged to obtain a supernatant. The supernatant was concentrated at 50℃ and -0.08MPa until the solid content of the solution reached 10%. The solution was then freeze-dried to obtain collagen.
[0025] Comparative Example 3 The only difference from Example 1 is that step (1) in Comparative Example 3 is as follows: (1) Preparation of collagen Cod (Pacific cod) Gadus macrocephalus Clean the fish skin, drain the water, and cut it into small pieces of 1cm×1cm to obtain the processed fish skin; At 28-30℃, the treated fish skin was completely immersed in a 70% ethanol solution for 4 hours. After that, the fish skin was rinsed with water. Then, the rinsed fish skin was completely immersed in a 0.08% citric acid solution for 4 hours. After rinsing with water, the soaked fish skin was obtained. At 40℃, buckwheat (specifically bitter buckwheat) is placed in a container. Fagopyrum tataricum (L.) Gaertn.) was mixed with water at a mass ratio of 1:8 and soaked for 10 hours. After filtration, buckwheat filtrate was obtained. Soaked fish skin, papain and buckwheat filtrate were mixed and homogenized at a mass ratio of 10:8:90, and then enzymatically hydrolyzed at 60℃ for 8 hours. After enzyme inactivation and solid-liquid separation, crude collagen extract was obtained. Collagen crude liquid and ammonium sulfate (food grade) were mixed at a mass ratio of 100:1 and stirred until homogeneous. The mixture was then allowed to stand at 28-30℃ for 4 hours and centrifuged to obtain a precipitate. The precipitate was then mixed with phosphate buffer (pH 7.0, concentration 150mM) at a mass-to-volume ratio of 0.5g:20mL. The mixture was then chromatographed with G-75, 100, 150 dextran. The protein eluate was collected and centrifuged to obtain a supernatant. The supernatant was concentrated at 50℃ and -0.08MPa until the solid content of the solution was 10%. The solution was then freeze-dried to obtain collagen.
[0026] Comparative Example 4 The only difference from Example 1 is that step (1) in Comparative Example 4 is as follows: (1) Preparation of collagen Cod (Pacific cod) Gadus macrocephalus Clean the fish skin, drain the water, and cut it into small pieces of 1cm×1cm to obtain the processed fish skin; At 40℃, buckwheat (specifically bitter buckwheat) is placed in a container. Fagopyrum tataricum (L.) Gaertn.) was mixed with water at a mass ratio of 1:8 and soaked for 10 hours. After filtration, buckwheat filtrate was obtained. The treated fish skin, compound enzyme (the compound enzyme is obtained by mixing papain and phospholipase C at a mass ratio of 0.5:1) and buckwheat filtrate at a mass ratio of 10:8:90 were mixed and homogenized, and then enzymatically hydrolyzed at 60℃ for 8 hours. After enzyme inactivation and solid-liquid separation, crude collagen extract was obtained. Collagen crude liquid and ammonium sulfate (food grade) were mixed at a mass ratio of 100:1 and stirred until homogeneous. The mixture was then allowed to stand at 28-30℃ for 4 hours and centrifuged to obtain a precipitate. The precipitate was then mixed with phosphate buffer (pH 7.0, concentration 150mM) at a mass-to-volume ratio of 0.5g:20mL. The mixture was then chromatographed with G-75, 100, 150 dextran. The protein eluate was collected and centrifuged to obtain a supernatant. The supernatant was concentrated at 50℃ and -0.08MPa until the solid content of the solution was 10%. The solution was then freeze-dried to obtain collagen.
[0027] Comparative Example 5 The only difference from Example 1 is that step (2) in Comparative Example 5 is as follows: (2) Preparation of gynecological gel By weight, the gynecological gel comprises the following ingredients: 0.5 parts carbomer, 0.09 parts sodium hydroxide, 6 parts collagen, 0.02 parts sodium hyaluronate, 0.5 parts isomaltooligosaccharide, 2 parts propylene glycol, 0.05 parts methylparaben, 0.02 parts ethylparaben, 0.1 parts hydrogenated lecithin, 1 part 1,3-butanediol, 5 parts glycerin, 1 part xanthan gum, and 83.72 parts water; Carbomer, sodium hydroxide, collagen, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, methylparaben, ethylparaben, hydrogenated lecithin, 1,3-butanediol, glycerin, xanthan gum, and water are mixed evenly to obtain a gynecological gel.
[0028] Antibacterial effect verification According to GB15979 The method in Appendix C of the 2002 "Hygienic Standard for Disposable Sanitary Products" was used to determine the antibacterial effects of each gynecological gel against Escherichia coli, Candida albicans, and Staphylococcus aureus using the gynecological gels prepared in Examples 1-2 and Comparative Examples 1-5. The results are shown in Table 1.
[0029] Table 1. Antibacterial effects of different gynecological gels
[0030] As can be seen from the data in Table 1, compared with the comparison, the gynecological gels prepared in Examples 1 and 2 have the best antibacterial effect, with an antibacterial rate of 97%-98% against Escherichia coli, 95%-97% against Candida albicans, and 90%-97% against Staphylococcus aureus.
[0031] Based on the above antibacterial experiment results, the gynecological gels prepared in Examples 1 and 2 were selected for the following verification: A sterile cotton swab was used to collect secretions from the upper third of the vaginal wall of a healthy woman. The secretions were spread onto MRS solid culture medium, anaerobically cultured, and single colonies isolated. After liquid amplification, the culture was centrifuged, and the supernatant was collected and labeled as *Lactobacillus*. The *Lactobacillus* were then reacted with a 1×10⁻⁶ solution... 8 Staphylococcus aureus at a volume ratio of 2:1 was mixed to obtain a mixed bacterial solution.
[0032] 1 mL of mixed bacterial solution and 1 mL of gynecological gel prepared in Example 1 were simultaneously inoculated into 500 mL of liquid culture medium (referred to as Example 1). 1 mL of mixed bacterial solution and 1 mL of gynecological gel prepared in Example 2 were simultaneously inoculated into 500 mL of liquid culture medium (referred to as Example 2). 1 mL of mixed bacterial solution was inoculated into 500 mL of liquid culture medium (referred to as blank control). After culturing under the same conditions for 24 h, the number of bacteria in each treatment was counted. The results are shown in Table 2.
[0033] Table 2. Quantities of various bacterial groups after co-culturing Lactobacillus and Staphylococcus aureus
[0034] As shown in Table 2, when the gynecological gel prepared in the examples is cultured with a mixed bacterial solution containing Lactobacillus and Staphylococcus aureus, it not only inhibits the increase in the number of Staphylococcus aureus but also promotes the increase in the number of beneficial Lactobacillus strains. Therefore, the gynecological gel prepared in this invention is beneficial for regulating the vaginal microecological balance.
[0035] Zebrafish firming effect verification The gynecological gels prepared in Examples 1-2 and Comparative Examples 1-5 were used as test subjects, with a concentration of 0.1% in the working solution. A blank control group and a positive control (TGF-β1, working concentration 100 ng / mL) were set up. Each test subject was considered a treatment, and 10 zebrafish were selected for each treatment. 5 mL of working solution was added to each well. Each treatment was incubated in a 28.5±0.5℃ incubator for 24 h before sampling. Total RNA was extracted from the zebrafish in each group, reverse transcribed, and amplified by quantitative real-time PCR. β-actin gene was used as an internal reference gene. -△△Ct The relative expression levels of the elastin gene (elna) in each group were calculated, and the average relative expression level was calculated. The results are shown in Table 3.
[0036] Table 3. Relative expression levels of elastin genes in each group
[0037] As shown in Table 3, compared with the comparative example, the gynecological gel prepared in the examples can significantly promote the expression of elastin genes, thus helping to improve the tightness and elasticity of the vaginal mucosa, with the best tightening effect.
[0038] In summary, the combination of carbomer, sodium hydroxide, collagen, and sodium hyaluronate, among other ingredients, results in a synergistic effect that promotes collagen fiber synthesis and repair, enhancing tissue elasticity. Therefore, it can be used for vaginal antibacterial effects, regulation of the vaginal microecological balance, and vaginal tightening.
[0039] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.
Claims
1. A gynecological gel for vaginal antibacterial, microecological balance regulation, and vaginal tightening, characterized in that, By weight, it includes the following raw materials: Carbomer 0.5-1 parts, sodium hydroxide 0.04-0.09 parts, collagen 1-3 parts, sodium hyaluronate 0.01-0.06 parts, isomaltooligosaccharide 0.5-1.5 parts, propylene glycol 2-5 parts, methylparaben 0.05-0.3 parts, ethylparaben 0.02-0.15 parts, hydrogenated lecithin 0.1-1.5 parts, 1,3-butanediol 1-6 parts, glycerin 3-5 parts, xanthan gum 0.5-1 parts, and water 70-90 parts.
2. The gynecological gel according to claim 1, characterized in that, By weight, it includes the following raw materials: Carbomer 0.5 parts, sodium hydroxide 0.09 parts, collagen 2 parts, sodium hyaluronate 0.02 parts, isomaltooligosaccharide 0.5 parts, propylene glycol 2 parts, methylparaben 0.05 parts, ethylparaben 0.02 parts, hydrogenated lecithin 0.1 parts, 1,3-butanediol 1 part, glycerin 5 parts, xanthan gum 1 part, and water 87.72 parts.
3. The gynecological gel according to claim 1 or 2, characterized in that, The preparation method of the collagen includes: S1. Soak the cod skin in ethanol solution and citric acid solution in turn to obtain soaked fish skin; S2. Fish skin, compound enzyme and buckwheat filtrate are mixed and enzymatically hydrolyzed, and after enzyme inactivation and solid-liquid separation, a crude collagen extract is obtained; the compound enzyme includes: papain and phospholipase C; S3. Mix the crude collagen liquid and ammonium sulfate, stir, let stand, and centrifuge to obtain a precipitate; S4. After mixing the precipitate with phosphate buffer, perform chromatography and centrifugation to obtain the supernatant; concentrate and freeze-dry the supernatant to obtain collagen.
4. The gynecological gel according to claim 3, characterized in that, In step S1, the volume concentration of the ethanol solution is 70%-80%; The citric acid solution has a mass concentration of 0.03%-0.08%.
5. The gynecological gel according to claim 3, characterized in that, In step S2, papain and phospholipase C are mixed at a mass ratio of 0.5-0.8:1 to obtain a complex enzyme.
6. The gynecological gel according to claim 3, characterized in that, In step S2, the mass ratio of fish skin, compound enzyme and buckwheat filtrate is 10-15:5-8:75-90; The temperature for the mixed enzymatic hydrolysis is 50-60℃, and the time is 8-10h.
7. The gynecological gel according to claim 3 or 6, characterized in that, The buckwheat filtrate is prepared by mixing buckwheat and water at a mass ratio of 1:4-8 at 40-45℃ and soaking for 10-12 hours, followed by filtration to obtain the buckwheat filtrate.
8. The gynecological gel according to claim 3, characterized in that, In step S3, during the settling process, the mass ratio of crude collagen solution to ammonium sulfate is 100:1-1.
5. The standing time is 2-4 hours, and the temperature is 25-30℃.
9. The gynecological gel according to claim 3, characterized in that, In step S4, the mass-to-volume ratio of the precipitate to the phosphate buffer is 0.1-0.5 g: 20-30 mL; The phosphate buffer solution has a pH of 7.0-7.5 and a concentration of 150-200 mM.
10. A method for preparing the gynecological gel according to any one of claims 1-9, characterized in that, Includes the following steps: Carbomer, sodium hydroxide, collagen, sodium hyaluronate, isomaltooligosaccharide, propylene glycol, methylparaben, ethylparaben, hydrogenated lecithin, 1,3-butanediol, glycerin, xanthan gum, and water are mixed evenly to obtain a gynecological gel.