Genetically engineered escherichia coli plasmid expression culture medium and fermentation process thereof
By optimizing the E. coli culture medium and fermentation process, the problem of low plasmid DNA production efficiency was solved, resulting in a significant increase in plasmid DNA yield and a reduction in cost, thus meeting the needs of the nucleic acid vaccine industrialization.
CN122146499APending Publication Date: 2026-06-05SICHUAN BAINUOJI TECH CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SICHUAN BAINUOJI TECH CO LTD
- Filing Date
- 2026-03-23
- Publication Date
- 2026-06-05
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Figure CN122146499A_ABST
Abstract
The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.
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